Journal of Equine Science最新文献

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Radiographic texture of the trabecular bone of the proximal phalanx in horses with metacarpophalangeal osteoarthritis. 患有掌指关节骨性关节炎的马匹近节指骨骨小梁的放射纹理。
Journal of Equine Science Pub Date : 2024-07-01 Epub Date: 2024-07-03 DOI: 10.1294/jes.35.21
Lorena de Oliveira Pereira, Anderson Fernando DE Souza, Julio David Spagnolo, Ana Lúcia Miluzzi Yamada, Daniela Miranda Richarte de Andrade Salgado, André Luis do Valle DE Zoppa
{"title":"Radiographic texture of the trabecular bone of the proximal phalanx in horses with metacarpophalangeal osteoarthritis.","authors":"Lorena de Oliveira Pereira, Anderson Fernando DE Souza, Julio David Spagnolo, Ana Lúcia Miluzzi Yamada, Daniela Miranda Richarte de Andrade Salgado, André Luis do Valle DE Zoppa","doi":"10.1294/jes.35.21","DOIUrl":"10.1294/jes.35.21","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a prevalent condition in horses, leading to changes in trabecular bone structure and radiographic texture. Although fractal dimension (FD) and lacunarity have been applied to quantify these changes in humans, their application in horses remains nascent. This study evaluated the use of FD, bone area fraction (BA/TA), and lacunarity in quantifying trabecular bone differences in the proximal phalanx (P1) in 50 radiographic examinations of equine metacarpophalangeal joints with varying OA degrees. In the dorsopalmar view, regions of interest were defined in the trabecular bone of the proximal epiphysis, medial and lateral to the sagittal groove of P1. Lower BA/TA values were observed medially in horses with severe OA (P=0.003). No significant differences in FD and lacunarity were found across OA degrees (P>0.1). FD, BA/TA, and lacunarity were not effective in identifying radiographic texture changes in the P1 trabecular bone in horses with different metacarpophalangeal OA degrees.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Can Nigerian horse owners effectively estimate body condition and cresty neck scores? 尼日利亚马主能否有效估计马匹的身体状况和嵴椎评分?
Journal of Equine Science Pub Date : 2024-03-01 Epub Date: 2024-03-19 DOI: 10.1294/jes.35.9
Olumide Odunayo Akinniyi, Philip Wayuta Mshelia, Richard Emmanuel Edeh
{"title":"Can Nigerian horse owners effectively estimate body condition and cresty neck scores?","authors":"Olumide Odunayo Akinniyi, Philip Wayuta Mshelia, Richard Emmanuel Edeh","doi":"10.1294/jes.35.9","DOIUrl":"10.1294/jes.35.9","url":null,"abstract":"<p><p>A horse's body condition impacts every aspect of its life, including reproduction, performance, and health. Obesity and equine metabolic syndrome (EMS) are common in Nigeria; hence, early identification of a horse's obesity status by the owner is key to avoiding associated health issues such as EMS. Our study aimed to determine whether horse owners could effectively estimate their horses' body condition scores (BCSs) and cresty neck scores (CNSs). A total of 50 adult sedentary West African Barb horses owned by 50 different people were enrolled in the study. Body condition scores (BCSs) and cresty neck scores (CNSs) were assessed on scales of 1-9 and 0-5, respectively, by an experienced veterinarian and owners. The latter had no veterinary background, received no prior education, and assessed their horses based on provided visual aids and descriptions. The BCS estimates of the experienced veterinarian and owners were slightly in agreement (k=0.209), while there was moderate agreement (k=0.547) between the CNS estimates of the experienced veterinarian and the owners. The proportion of obesity based on the experienced veterinarian's estimation (32%) was higher than that of the owners (18%). The proportion of nuchal crest adiposity based on the experienced veterinarian's (38%) estimation was slightly lower than that of the owners (42%). A larger proportion of the owners underestimated their horses' BCSs and CNSs. We conclude that owners cannot effectively estimate their horses' BCSs and CNSs. It is imperative that they are informed about the health issues associated with obesity and are taught how to effectively estimate BCS and CNS.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-suppurative and necrotizing testicular arteritis in the male reproductive organs of a heavy draft horse. 一匹重型草马的雄性生殖器官中出现非化脓性坏死性睾丸动脉炎。
Journal of Equine Science Pub Date : 2024-03-01 Epub Date: 2024-03-19 DOI: 10.1294/jes.35.15
Yusuke Tanaka, Kenichi Watanabe, Akiko Takeyama, Masaaki Tagami, Hayato Hamano, Natsuko Fukumoto, Yasuo Nambo, Yoshiyasu Kobayashi
{"title":"Non-suppurative and necrotizing testicular arteritis in the male reproductive organs of a heavy draft horse.","authors":"Yusuke Tanaka, Kenichi Watanabe, Akiko Takeyama, Masaaki Tagami, Hayato Hamano, Natsuko Fukumoto, Yasuo Nambo, Yoshiyasu Kobayashi","doi":"10.1294/jes.35.15","DOIUrl":"10.1294/jes.35.15","url":null,"abstract":"<p><p>Equine testicular arteritis commonly occurs as a consequence of the migration of nematode larvae or equine arteritis virus (EAV) infection. However, testicular arteritis without evidence of these infections has been reported, and the underlying pathogenesis remains unclear. We encountered testicular arteritis without evidence of nematode or EAV infection in a 3-year-old male heavy draft horse with scrotal enlargement. Grossly, the volume of the pampiniform plexus was markedly increased due to edema. Histologically, non-suppurative and necrotizing testicular arteritis, characterized by lymphocyte infiltration and fibrinoid necrosis of the arterial walls, was diffusely observed in the spermatic cord, pampiniform plexus (most severe), testis, and epididymis. We were unable to identify the cause of arteritis, such as a viral infection or autoimmune abnormality.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
3D imaging and body measurement of riding horses using four scanners simultaneously. 同时使用四台扫描仪对骑马进行三维成像和身体测量。
Journal of Equine Science Pub Date : 2024-03-01 Epub Date: 2024-03-19 DOI: 10.1294/jes.35.1
Akihiro Matsuura, Suzuka Torii, Yuki Ojima, Yoshio Kiku
{"title":"3D imaging and body measurement of riding horses using four scanners simultaneously.","authors":"Akihiro Matsuura, Suzuka Torii, Yuki Ojima, Yoshio Kiku","doi":"10.1294/jes.35.1","DOIUrl":"10.1294/jes.35.1","url":null,"abstract":"<p><p>Although there have been advances in the technology for measuring horse body size with stereoscopic three-dimensional (3D) scanners, previously reported methods with a single scanner still face a significant challenge: the time necessary for scanning is too long for the horses to remain stationary. This study attempted to scan the horse simultaneously from four directions using four scanners in order to complete the scans in a short amount of time and then combine the images from the four scans on a computer into one whole image of each horse. This study also compared body measurements from the combined 3D images with those taken from conventional manual measurements. Nine riding horses were used to construct stereoscopic composite images, and the following 10 measurements were taken: height at the withers, back, and croup; chest depth; width of the chest (WCh), croup, and waist; girth circumference, cannon circumference (CaC), and body length. The same 10 measurements were taken by conventional manual methods. Relative errors ranged from -1.89% to 7.05%. The correlation coefficient between manual and 3D measurements was significant for all body measurements (P<0.01) except for WCh and CaC. A simple regression analysis of all body measurements revealed a strong correlation (P<0.001, R<sup>2</sup>=0.9994, root-mean-square error=1.612). Simultaneous scanning with four devices from four directions reduced the scanning time from 60 sec with one device to 15 sec. This made it possible to perform non-contact body measurements even on incompletely trained horses who could not remain stationary for long periods of time.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serum amino acid profiles in clinically normal Noma horses. 临床正常的坏疽性口炎马的血清氨基酸谱。
Journal of Equine Science Pub Date : 2024-01-01 Epub Date: 2024-07-03 DOI: 10.1294/jes.35.29
Keiichi Hisaeda, Tetsushi Ono, Sho Kadekaru, Akihisa Hata, Takako Shimokawa Miyama, Kenji Kutara, Keisuke Sugimoto, Yasuharu Hiasa, Emi Ohzawa, Tetsuo Kunieda, Eri Iwata, Hitoshi Kitagawa
{"title":"Serum amino acid profiles in clinically normal Noma horses.","authors":"Keiichi Hisaeda, Tetsushi Ono, Sho Kadekaru, Akihisa Hata, Takako Shimokawa Miyama, Kenji Kutara, Keisuke Sugimoto, Yasuharu Hiasa, Emi Ohzawa, Tetsuo Kunieda, Eri Iwata, Hitoshi Kitagawa","doi":"10.1294/jes.35.29","DOIUrl":"10.1294/jes.35.29","url":null,"abstract":"<p><p>Plasma or serum amino acids are used to evaluate nutritional status and metabolic disorders. In this study, we aimed to set reference values of serum amino acid concentrations in the Noma horse, a Japanese native horse. Thirty-one horses were classified into six age groups: neonatal foal (0-4 days), foal (0.5-1 years), youth (5 years), middle age (10 years), old (15 years), and extra-old (>20 years). Horses >5 years of age were analyzed together as the adult group. In the adult horses, there were no significant differences among the serum amino acid concentrations of each age group. The foal group had higher concentrations of alanine, aspartic acid, glutamic acid, α-aminoadipic acid, and 3-methyl-histidine than the adult group. The neonatal foal group had higher serum concentrations of phenylalanine, lysine, alanine, proline, aspartic acid, glutamic acid, β-alanine, and β-amino-iso-butyric acid and lower tryptophan concentrations and Fischer's ratios than the adult group. The neonatal foal group had higher β-amino-iso-butyric acid concentrations and lower tryptophan and 3-methyl-histidine concentrations than the foal group. Therefore, reference values might be set separately in neonatal foals, foals, and adult horses. The data for the serum amino acid concentrations can be used for health care through physiological and pathological evaluations in Noma horses.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pharmacokinetic/pharmacodynamic analysis of cephalothin after intramuscular administration in Thoroughbred horses. 头孢噻吩在纯血马肌肉注射后的药代动力学/药效学分析。
Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.111
Taisuke Kuroda, Yohei Minamijima, Hidekazu Niwa, Hiroshi Mita, Norihisa Tamura, Kentaro Fukuda, Minoru Ohta
{"title":"Pharmacokinetic/pharmacodynamic analysis of cephalothin after intramuscular administration in Thoroughbred horses.","authors":"Taisuke Kuroda, Yohei Minamijima, Hidekazu Niwa, Hiroshi Mita, Norihisa Tamura, Kentaro Fukuda, Minoru Ohta","doi":"10.1294/jes.34.111","DOIUrl":"10.1294/jes.34.111","url":null,"abstract":"<p><p>A pharmacokinetic/pharmacodynamic (PK/PD) approach was used to determine a dosage regimen of cephalothin (CET) after intramuscular (IM) administration in horses. CET plasma concentrations were measured in eight horses after a single IM administration of 11 mg/kg bwt of CET. The data were modeled using a nonlinear mixed-effect model, and the probability of target attainment (PTA) of the PK/PD target was calculated for 5,000 horses generated by Monte Carlo simulations. IM administrations of CET at 11 mg/kg bwt q 8 hr and q 6 hr achieved a PTA of 90% against the MIC<sub>90</sub> of S. zooepidemicus and S. aureus, respectively, and were considered to be effective dosage regimens. The total dose for the IM administration recommended in this study was lower than that for intravenous (IV) administration in previous studies.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10806359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139564980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of concentrate levels on intestinal fermentation and the microbial profile in Japanese draft horses. 精料水平对日本草食马肠道发酵和微生物概况的影响。
Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.101
Rintaro Yano, Tomoe Moriyama, Miho Fujimori, Takehiro Nishida, Masaaki Hanada, Naoki Fukuma
{"title":"Effects of concentrate levels on intestinal fermentation and the microbial profile in Japanese draft horses.","authors":"Rintaro Yano, Tomoe Moriyama, Miho Fujimori, Takehiro Nishida, Masaaki Hanada, Naoki Fukuma","doi":"10.1294/jes.34.101","DOIUrl":"10.1294/jes.34.101","url":null,"abstract":"<p><p>In racehorses, feeding a high-concentrate diet could cause abnormal fermentation in the hindgut. This feeding management regime is not suitable for the nutritional physiology of horses. However, studies on the hindgut environment have yet to be reported in Japanese draft horses, so feeding management needs to be investigated in these horses. Therefore, the objective of this study was to investigate the effects of a high-concentrate diet on hindgut fermentation in Japanese draft horses. Feces were collected from 20 male Japanese draft horses managed by two stables with different feeding designs (65% weight ratio of concentrate feed, HC; 50% weight ratio of concentrate, MC), and fecal metabolic characteristics and the microbiome were analyzed. Higher lactate concentrations and lower fecal pH levels were observed in the HC group (P=0.0011, P=0.0192, respectively). Fecal microbiome analysis revealed a decrease in microbial diversity (P=0.0360) and an increase in the relative abundance of Streptococcus lutetiensis/equinus/infantarius (P=0.0011) in the HC group. On the other hand, fibrolytic bacteria in the MC group had similarities with Clostridium sacchalolyticum and Ruminococcus albus. This study revealed that overfeeding of concentrates induced abnormal fermentation in the hindgut of Japanese draft horses. This suggests that the establishment of a feeding design based on not only the chemical compositions of feeds but also microbial dynamics is needed.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10806360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139564924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Postmortem magnetic resonance imaging findings of tricuspid atresia with ventricular and atrial septal defects and subvalvular pulmonic stenosis in a Japanese native Noma horse. 一匹日本原产野马的三尖瓣闭锁伴心室和房间隔缺损以及瓣下肺动脉狭窄的尸检磁共振成像结果。
Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.121
Kenji Kutara, Sho Kadekaru, Keiichi Hisaeda, Keisuke Sugimoto, Tetsushi Ono, Yoichi Inoue, Shinichi Nakamura, Ryohei Yoshitake, Emi Ohzawa, Akira Goto, Eri Iwata, Kenichi Shibano, Yumi Une, Hitoshi Kitagawa
{"title":"Postmortem magnetic resonance imaging findings of tricuspid atresia with ventricular and atrial septal defects and subvalvular pulmonic stenosis in a Japanese native Noma horse.","authors":"Kenji Kutara, Sho Kadekaru, Keiichi Hisaeda, Keisuke Sugimoto, Tetsushi Ono, Yoichi Inoue, Shinichi Nakamura, Ryohei Yoshitake, Emi Ohzawa, Akira Goto, Eri Iwata, Kenichi Shibano, Yumi Une, Hitoshi Kitagawa","doi":"10.1294/jes.34.121","DOIUrl":"10.1294/jes.34.121","url":null,"abstract":"<p><p>The necropsy of a 2-day-old Noma horse that died of weakness showed an enlarged cardiac base and a narrow cardiac apex, suggesting cardiac malformation. The excised heart underwent imaging to investigate its luminal structure. On three-dimensional magnetic resonance imaging, the right atrium and right ventricle were discontinuous. The right atrium communicated with the left atrium and the left ventricle communicated with the right ventricle. The lumen narrowed near the pulmonary artery valve. Since the same findings were observed on gross examination, the foal was diagnosed with tricuspid atresia with ventricular and atrial septal defects, along with subvalvular pulmonic stenosis.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10806361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139564981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic characterization of Japanese native horse breeds by genotyping variants that are associated with phenotypic traits. 通过对与表型特征相关的变体进行基因分型,确定日本本土马匹品种的遗传特征。
Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.115
Ibuki Saito, Kotono Nakamura, Teruaki Tozaki, Kazuki Hano, Masaki Takasu
{"title":"Genetic characterization of Japanese native horse breeds by genotyping variants that are associated with phenotypic traits.","authors":"Ibuki Saito, Kotono Nakamura, Teruaki Tozaki, Kazuki Hano, Masaki Takasu","doi":"10.1294/jes.34.115","DOIUrl":"10.1294/jes.34.115","url":null,"abstract":"<p><p>Concerns have been raised about the loss of genetic diversity in Japanese native horses because of their declining populations. In this study, we investigated the genetic variation of four genes, myostatin (MSTN), ligand-dependent nuclear receptor corepressor like (LCORL), doublesex and mab-3 related transcription factor 3 (DMRT3), and 5-hydroxytryptamine receptor 1A (HTR1A), which are associated with horse phenotypic traits, in six Japanese horse breeds (Hokkaido, Kiso, Noma, Misaki, Tokara, and Yonaguni). MSTN, LCORL, DMRT3, and HTR1A showed polymorphisms in the Kiso; Hokkaido and Noma; Hokkaido; and Kiso, Tokara, and Yonaguni breeds, respectively. The Misaki did not show polymorphisms in any of the genes. This study may serve as a basis for developing future breeding strategies focusing on traits in Japanese native horses.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10806362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139564957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells. 白藜芦醇及其类似物对马间充质干/基质细胞细胞周期的影响。
Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.67
Norihisa Tamura, Neda Heidari, Richard G A Faragher, Roger K W Smith, Jayesh Dudhia
{"title":"Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.","authors":"Norihisa Tamura,&nbsp;Neda Heidari,&nbsp;Richard G A Faragher,&nbsp;Roger K W Smith,&nbsp;Jayesh Dudhia","doi":"10.1294/jes.34.67","DOIUrl":"https://doi.org/10.1294/jes.34.67","url":null,"abstract":"<p><p>Resveratrol (RSV; trans-3,5,4'-trihydroxystilbene) strongly activates sirtuin 1, and it and its analogue V29 enhance the proliferation of mesenchymal stem/stromal cells (MSCs).Although culture medium containing 5-azacytydine and RSV inhibits senescence of adipose tissue-derived MSCs isolated from horses with metabolic syndrome, few studies have reported the effects of RSV on equine bone marrow-derived MSCs (eBMMSCs) isolated from horses without metabolic syndrome. The aim of this study was to investigate the effects of RSV and V29 on the cell cycle of eBMMSCs. Following treatment with 5 µM RSV or 10 µM V29, the cell proliferation capacity of eBMMSCs derived from seven horses was evaluated by EdU (5-ethynyl-2'-deoxyuridine) and Ki-67 antibody assays. Brightfield images of cells and immunofluorescent images of EdU, Ki-67, and DAPI staining were recorded by fluorescence microscopy, and the number of cells positive for each was quantified and compared by Friedman's test at P<0.05. The growth fraction of eBMMSCs was significantly increased by RSV and V29 as measured by the EdU assay (control 28.1% ± 13.8%, V29 31.8% ± 14.6%, RSV 32.0% ± 10.8%; mean ± SD; P<0.05) but not as measured by the Ki-67 antibody assay (control 27.0% ± 11.2%, V29 27.4% ± 10.8%, RSV 27.7% ± 6.8%). RSV and V29 promoted progression of the cell cycle of eBMMSCs into the S phase and may be useful for eBMMSC expansion.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0d/8c/jes-34-067.PMC10534064.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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