Journal of Equine Science最新文献_第2页

Genetic characterization of Japanese native horse breeds by genotyping variants that are associated with phenotypic traits. 通过对与表型特征相关的变体进行基因分型，确定日本本土马匹品种的遗传特征。

Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.115

Ibuki Saito, Kotono Nakamura, Teruaki Tozaki, Kazuki Hano, Masaki Takasu

引用次数: 0

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells. 白藜芦醇及其类似物对马间充质干/基质细胞细胞周期的影响。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.67

Norihisa Tamura, Neda Heidari, Richard G A Faragher, Roger K W Smith, Jayesh Dudhia

{"title":"Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.","authors":"Norihisa Tamura, Neda Heidari, Richard G A Faragher, Roger K W Smith, Jayesh Dudhia","doi":"10.1294/jes.34.67","DOIUrl":"https://doi.org/10.1294/jes.34.67","url":null,"abstract":"Resveratrol (RSV; trans-3,5,4'-trihydroxystilbene) strongly activates sirtuin 1, and it and its analogue V29 enhance the proliferation of mesenchymal stem/stromal cells (MSCs).Although culture medium containing 5-azacytydine and RSV inhibits senescence of adipose tissue-derived MSCs isolated from horses with metabolic syndrome, few studies have reported the effects of RSV on equine bone marrow-derived MSCs (eBMMSCs) isolated from horses without metabolic syndrome. The aim of this study was to investigate the effects of RSV and V29 on the cell cycle of eBMMSCs. Following treatment with 5 µM RSV or 10 µM V29, the cell proliferation capacity of eBMMSCs derived from seven horses was evaluated by EdU (5-ethynyl-2'-deoxyuridine) and Ki-67 antibody assays. Brightfield images of cells and immunofluorescent images of EdU, Ki-67, and DAPI staining were recorded by fluorescence microscopy, and the number of cells positive for each was quantified and compared by Friedman's test at P<0.05. The growth fraction of eBMMSCs was significantly increased by RSV and V29 as measured by the EdU assay (control 28.1% ± 13.8%, V29 31.8% ± 14.6%, RSV 32.0% ± 10.8%; mean ± SD; P<0.05) but not as measured by the Ki-67 antibody assay (control 27.0% ± 11.2%, V29 27.4% ± 10.8%, RSV 27.7% ± 6.8%). RSV and V29 promoted progression of the cell cycle of eBMMSCs into the S phase and may be useful for eBMMSC expansion.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0d/8c/jes-34-067.PMC10534064.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Construction of an individual identification panel for horses using insertion and deletion markers. 使用插入和删除标记构建马的个体识别面板。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-08-09 DOI: 10.1294/jes.34.83

Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Shun-Ichi Nagata

{"title":"Construction of an individual identification panel for horses using insertion and deletion markers.","authors":"Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Shun-Ichi Nagata","doi":"10.1294/jes.34.83","DOIUrl":"https://doi.org/10.1294/jes.34.83","url":null,"abstract":"Individual identification and paternity testing are important for avoiding inbreeding in the management of small populations of wild and domestic animals. In horse racing industries, they are extremely important for identifying and registering individuals and doping control to ensure fair competition. In this study, we constructed an individual identification panel for horses by using insertion and deletion (INDEL) markers. The panel included 39 INDEL markers selected from a whole-genome INDEL database. Genotyping of 89 Thoroughbreds showed polymorphisms with minor allele frequencies (MAFs) of 0.180-0.489 in all markers. The total probability of exclusion for paternity testing, power of discrimination, and probability of identity were 0.9994271269, >0.9999999999, and 0.9999999987, respectively. The panel was applied to 13 trios (sires, dams, and foals), and no contradictions were observed in genetic inheritance among the trios. When this panel was applied to the trios (52 trios) containing false fathers, an average of 7.3 markers excluded parentage relationships. In addition, genomic DNA extracted from the urine of six horses was partially genotyped for 39 markers, and 6-28 markers were successfully genotyped. The newly constructed panel has two advantages: a low marker mutation rate compared with short tandem repeats and a genotyping procedure that is as simple as short tandem repeat typing compared with single nucleotide variant typing. This panel can be applied for individual identification, paternity determination, and urine-sample identification in Thoroughbred horses.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/3b/jes-34-083.PMC10534061.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41172379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements. 马绞痛的严重程度与氧化应激生物标志物、急性期蛋白质和某些微量元素的关系。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.73

Ali Hajimohammadi, Mohsen Ghane, Masoud Ghari Tehrani, Benyamin Paravar, Ahmadreza Mirzaei, SeyedAmin Razavi, Mohammad Nikzad

{"title":"Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements.","authors":"Ali Hajimohammadi, Mohsen Ghane, Masoud Ghari Tehrani, Benyamin Paravar, Ahmadreza Mirzaei, SeyedAmin Razavi, Mohammad Nikzad","doi":"10.1294/jes.34.73","DOIUrl":"10.1294/jes.34.73","url":null,"abstract":"Sixty-one horses were enrolled in this study and divided into 3 different groups according to their severity of colic (heart rate, oral mucous membrane color, and abdominal distention): a strangulating colic (SC) group (n=21), non-strangulating colic (NC) group (n=20), and control group (n=20) consisting of randomly selected normal horses without signs of colic. The serum concentrations of haptoglobin, tumor necrosis factor-α (TNFα), nitric oxide (NO), malondialdehyde (MDA), zinc, iron, and copper were evaluated in all horses. The average concentration of TNFα in the SC group was higher than that in the control group (P<0.001). The TNFα concentration was higher in the NC group compared with the control group (P<0.001). Furthermore, the average concentration of TNFα tended to be higher in the SC group compared with the NC group (P=0.052). The average concentration of haptoglobin in the SC group was higher than that in the control group (P<0.001). The average concentration of NO was higher in the SC group compared with the NC group. (P=0.016) The average concentration of MDA was higher in the SC group compared with the control group (P=0.042). Furthermore, the concentration of MDA was higher in the SC group compared with the NC group (P=0.048). TNFα in horses with signs of colic may be a reliable indicator of prognosis and the severity of clinical signs. The haptoglobin concentration may be a useful marker in cases where animals are referred to clinicians a few days after the onset of colic. The concentrations of MDA and NO should be interpreted with caution.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8e/50/jes-34-073.PMC10534060.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41152614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence of surgical site infection after internal fixation of the first phalangeal bone and the third metacarpal/metatarsal bone fractures in Thoroughbred racehorses. 纯种赛马第一指骨和第三掌骨/跖骨骨折内固定后手术部位感染的发生率。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.61

Hiroshi Mita, Taisuke Kuroda, Hidekazu Niwa, Norihisa Tamura, Kentaro Fukuda, Minoru Ohta

{"title":"Incidence of surgical site infection after internal fixation of the first phalangeal bone and the third metacarpal/metatarsal bone fractures in Thoroughbred racehorses.","authors":"Hiroshi Mita, Taisuke Kuroda, Hidekazu Niwa, Norihisa Tamura, Kentaro Fukuda, Minoru Ohta","doi":"10.1294/jes.34.61","DOIUrl":"10.1294/jes.34.61","url":null,"abstract":"Surgical site infection (SSI) is one of the major complications of equine fracture surgery. The purpose of this study was to investigate the incidence of and risk factors for SSI after internal fixation of the first phalangeal bone (P1) and the third metacarpal/metatarsal bone (MC3/MT3) fractures in Thoroughbred racehorses. Between 2011 and 2020, 451 cases underwent surgery with screws or a locking compression plate (LCP) for sagittal fractures of P1 or condylar fractures of MC3/MT3. Overall, 2.9% (13/451) of the cases developed an SSI. The incidence was significantly higher in plate fixation (21.4%) than in screw fixation (2.3%). There was no significant association with other variables, such as sex, age, number of screws, experience of surgeon, or prophylactic antimicrobials. The median duration of hospitalization for screw fixation was 14 days without an SSI and 20 days with an SSI, and those for plate fixation were 26 and 25-88 days, respectively, indicating that the development of SSI prolongs the duration of hospitalization. On the other hand, there were no significant differences in discharge and race resumption rates between cases with and without an SSI. These data indicate that the incidence of SSI in this study was low and that it was higher following plate fixation than screw fixation.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/91/02/jes-34-061.PMC10534062.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41118457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of equine piroplasmosis in the Tokyo 2020 Olympic Games. 2020年东京奥运会上的一例马梨状体病。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.93

Hiroko Aida, Jonathan H Foreman, Akihiro Ochi, Yoshimasa Takizawa, Takashi Yamanaka

引用次数: 0

Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests. 适合基因兴奋剂检测的血浆样品的最佳储存和使用程序的研究。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.21

Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Yuji Takahashi, Shun-Ichi Nagata

{"title":"Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests.","authors":"Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Yuji Takahashi, Shun-Ichi Nagata","doi":"10.1294/jes.34.21","DOIUrl":"https://doi.org/10.1294/jes.34.21","url":null,"abstract":"Gene doping, which is prohibited in horseracing and equestrian sports, can be performed by introducing exogenous genes, known as transgenes, into the bodies of postnatal animals. To detect exogenous genes, a method utilizing quantitative polymerase chain reaction (qPCR) with a hydrolysis probe was developed to test whole blood and plasma samples, thereby protecting the fairness of competition and the rights of stakeholders in horseracing and equestrian sports. Therefore, we aimed to develop sample storage methods suitable for A and B samples in gene doping tests using blood. For sample A, sufficient qPCR detection was demonstrated after refrigeration for 1 to 2 weeks post collection. For sample B, the following procedures were confirmed to be suitable for storage: 1) centrifugation after sample receipt, 2) frozen storage, 3) natural thawing at room temperature, and 4) centrifugation without mixing blood cell components. Our results indicated that long-term cryopreservation yielded good plasma components from frozen blood samples even though it destroyed blood cells, indicating its applicability to the gene doping test using sample B, which can be stored for later use. Sample storage procedures are as important as detection methods in doping tests. Therefore, the series of procedures that we evaluated in this study will contribute to the efficient performance of gene doping tests through qPCR using blood samples.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e3/c6/jes-34-021.PMC10315635.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunohistochemical markers for equine granulosa cell tumors: a pilot study. 马颗粒细胞肿瘤的免疫组织化学标记物:一项初步研究。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.37

Aurelia Dolin, Peter Schweiger, Martin Waselau, Monika Egerbacher, Ingrid Walter

引用次数: 0

Efficiency of round bale feeders: comparison of Tombstone versus Hay Saver. 圆捆喂料机的效率:墓碑与干草节省器的比较。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.51

Kendall A Hyde, Annabelle Altman, Robin Banasek, Melba O Gastal, Eduardo L Gastal

引用次数: 0

Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses. 输血供马抗红细胞抗体阳性转化的监测。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.47

Hironaga Kakoi, Mio Kikuchi, Taichiro Ishige, Yuko Hirosawa, Shoko Tanaka, Shun-Ichi Nagata

{"title":"Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses.","authors":"Hironaga Kakoi, Mio Kikuchi, Taichiro Ishige, Yuko Hirosawa, Shoko Tanaka, Shun-Ichi Nagata","doi":"10.1294/jes.34.47","DOIUrl":"https://doi.org/10.1294/jes.34.47","url":null,"abstract":"To confirm the positive conversion of antibodies against erythrocyte antigens in horses, possible blood transfusion donor horses selected from draft horse populations were periodically monitored with an indirect antiglobulin (Coombs) test for approximately 3 years. In this study, 19 horses (16 females and 3 males) were investigated, and five mares showed alloantibodies during the monitoring period. Four mares were typically pregnant when positive conversion was detected, whereas no particular cause of conversion could be observed for one mare based on its clinical records. In the analyzed horses, most positive conversions were possibly due to pregnancy, as conversion occurred more often during this period than after parturition. Pregnancy is considered a key event for positive conversion. Additionally, in cases in which unknown causative sensitization is confirmed, continuous monitoring with a test to detect antibodies should be performed, even if the possible donor is selected and maintained.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/91/cf/jes-34-047.PMC10315640.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0