白藜芦醇及其类似物对马间充质干/基质细胞细胞周期的影响。

Q3 Veterinary
Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI:10.1294/jes.34.67
Norihisa Tamura, Neda Heidari, Richard G A Faragher, Roger K W Smith, Jayesh Dudhia
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引用次数: 1

摘要

白藜芦醇(RSV;反式-3,5,4’-三羟基二苯乙烯)强烈激活sirtuin 1,其及其类似物V29增强间充质干细胞/基质细胞(MSCs)的增殖。尽管含有5-氮杂胞苷和呼吸道合胞病毒的培养基抑制了从患有代谢综合征的马分离的脂肪组织来源的间充质干细胞的衰老,但很少有研究报道呼吸道合胞菌对从没有代谢综合征马分离的马骨髓来源的间质干细胞(eBMMSC)的影响。本研究的目的是研究RSV和V29对eBMMSCs细胞周期的影响。在用5µM RSV或10µM V29处理后,通过EdU(5-乙炔基-2'-脱氧尿苷)和Ki-67抗体测定来评估源自七匹马的eBMMSC的细胞增殖能力。通过荧光显微镜记录细胞的Brightfield图像和EdU、Ki-67和DAPI染色的免疫荧光图像,并通过P
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.

Resveratrol (RSV; trans-3,5,4'-trihydroxystilbene) strongly activates sirtuin 1, and it and its analogue V29 enhance the proliferation of mesenchymal stem/stromal cells (MSCs).Although culture medium containing 5-azacytydine and RSV inhibits senescence of adipose tissue-derived MSCs isolated from horses with metabolic syndrome, few studies have reported the effects of RSV on equine bone marrow-derived MSCs (eBMMSCs) isolated from horses without metabolic syndrome. The aim of this study was to investigate the effects of RSV and V29 on the cell cycle of eBMMSCs. Following treatment with 5 µM RSV or 10 µM V29, the cell proliferation capacity of eBMMSCs derived from seven horses was evaluated by EdU (5-ethynyl-2'-deoxyuridine) and Ki-67 antibody assays. Brightfield images of cells and immunofluorescent images of EdU, Ki-67, and DAPI staining were recorded by fluorescence microscopy, and the number of cells positive for each was quantified and compared by Friedman's test at P<0.05. The growth fraction of eBMMSCs was significantly increased by RSV and V29 as measured by the EdU assay (control 28.1% ± 13.8%, V29 31.8% ± 14.6%, RSV 32.0% ± 10.8%; mean ± SD; P<0.05) but not as measured by the Ki-67 antibody assay (control 27.0% ± 11.2%, V29 27.4% ± 10.8%, RSV 27.7% ± 6.8%). RSV and V29 promoted progression of the cell cycle of eBMMSCs into the S phase and may be useful for eBMMSC expansion.

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来源期刊
Journal of Equine Science
Journal of Equine Science Veterinary-Equine
CiteScore
1.60
自引率
0.00%
发文量
9
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