Journal of Equine Science最新文献_第5页

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests. 基于微流控定量pcr的马基因兴奋剂检测标准物质的设计与储存稳定性。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.125

Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Kanichi Kusano, Shun-Ichi Nagata

{"title":"Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests.","authors":"Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Kanichi Kusano, Shun-Ichi Nagata","doi":"10.1294/jes.32.125","DOIUrl":"https://doi.org/10.1294/jes.32.125","url":null,"abstract":"One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/a1/jes-32-125.PMC8731687.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equine nonneoplastic abnormal ovary in a draft mare with high serum anti-Müllerian hormone: a case study. 马非肿瘤性卵巢异常与高血清抗<s:1>勒氏激素:一个案例研究。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.147

Akira Goto, Masaaki Tagami, Fumiki Kato, Tsukasa Suzuki, Takashi Yamaga, Harutaka Murase, Fumio Sato, Munkhtuul Tsogtgerel, Tadamasa Niikura, Tomoe Moriyama, Akiko Chiba, Ken-Ichi Watanabe, Nao Tsuzuki, Yasuo Nambo

引用次数: 2

Effect of prostaglandin F2_α administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses. 前列腺素F2α对日本重驮马子宫多形核中性粒细胞计数的影响。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.117

Tadamasa Niikura, Munkhtuul Tsogtgerel, Chizuru Niikura, Haruna Wada, Seiya Kanzawa, Takashi Fujiwara, Yasuo Nambo

{"title":"Effect of prostaglandin F2α administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses.","authors":"Tadamasa Niikura, Munkhtuul Tsogtgerel, Chizuru Niikura, Haruna Wada, Seiya Kanzawa, Takashi Fujiwara, Yasuo Nambo","doi":"10.1294/jes.32.117","DOIUrl":"https://doi.org/10.1294/jes.32.117","url":null,"abstract":"The objective of this study was to examine the effect of prostaglandin F2α (dinoprost) and oxytocin administration on uterine polymorphonuclear neutrophil counts in the Japanese heavy draft mare. To compare polymorphonuclear neutrophil (PMN) counts in the endometrium, a total of 162 samples were collected from 54 estruses of 47 mares (before ovulation, day 0, and day 2) using a double-guarded cytology brush. Dinoprost (PG; 5 mg) was administered intramuscularly (i.m.) only once, on day 0, whereas oxytocin (OT; 20 U i.m.) was administered three times at 12-hr intervals starting on day 0. The plasma progesterone concentrations from days 0 to 14 were not different between the non-treatment (CON), PG treatment, and OT treatment groups. The PMN counts increased in all the groups from before administration to day 0 (CON, 0.90 to 3.55; PG, 1.20 to 8.45; and OT, 0.70 to 1.70; P=0.0014, 0.0046, and 0.0073, respectively). There was a significant decrease in PMNs from day 0 to day 2 only in the PG group (P=0.0073). The pregnancy rate was not different among the CON (12/18), PG (14/18), and OT (10/18) groups. The results of this study indicate that dinoprost can reduce uterine polymorphonuclear neutrophil counts.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/73/jes-32-117.PMC8731690.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum cortisol in mules intended for agricultural work. 用于农业工作的骡子血清中的皮质醇。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.153

Lady C Calixto, José R Martinez

引用次数: 0

Ultrasound-guided injections of the equine head and neck: review and expert opinion. 超声波引导下的马头和颈部注射：综述和专家意见。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.103

Jessica P Johnson, Tatiana Vinardell, Florent David

{"title":"Ultrasound-guided injections of the equine head and neck: review and expert opinion.","authors":"Jessica P Johnson, Tatiana Vinardell, Florent David","doi":"10.1294/jes.32.103","DOIUrl":"10.1294/jes.32.103","url":null,"abstract":"Ultrasound-guided injections can be used for a wide variety of conditions in the horse, including both diagnostic and therapeutic applications. Benefits of ultrasound guidance include more accurate deposition of injectate compared with blind approaches. Improved identification of vital structures, including nerves and blood vessels, allows their avoidance and thus reduces procedure-associated complications. Validation of such ultrasound-guided techniques has shown that they can be easily learnt by inexperienced veterinarians, assuming a proper knowledge of the sonographic anatomy. In many cases they can be employed in the field with a high level of accuracy, using widely available equipment, and with complete adherence to the sterility principles. Many ultrasound-guided injection techniques of the axial skeleton in the horse have been described in past years, enabling the equine veterinarian to perform more accurate treatments of specific anatomical areas. The goal of this review is to discuss diagnostic and therapeutic ultrasound-guided injection techniques of the skull and cervical spine in the horse, including those for the retrobulbar space, maxillary and inferior alveolar nerves, atlanto-occipital and atlanto-axial junctions, and cervical articular process joints, as well as the 1st cervical nerve, the C2 and C3 nerve plexus, and the 6th, 7th, and 8th cervical nerve roots.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/9c/jes-32-103.PMC8731684.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of the metacarpophalangeal joint angle on the transversal area and mean echogenicity of the superficial digital flexor tendon and suspensory ligament in gaited horses. 掌指关节角度对马指浅屈肌腱和悬韧带横截面积和平均回声的影响。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.135

Jackson Schade, Anderson Fernando de Souza, Lorenzo Costa Vincensi, Joandes Henrique Fonteque

{"title":"The influence of the metacarpophalangeal joint angle on the transversal area and mean echogenicity of the superficial digital flexor tendon and suspensory ligament in gaited horses.","authors":"Jackson Schade, Anderson Fernando de Souza, Lorenzo Costa Vincensi, Joandes Henrique Fonteque","doi":"10.1294/jes.32.135","DOIUrl":"https://doi.org/10.1294/jes.32.135","url":null,"abstract":"The objective of this study was to assess the influence of the metacarpophalangeal (MCP) joint angle on the transversal area (TA) and mean echogenicity (ME) of the superficial digital flexor tendon (SDFT) and suspensory ligament (SL) in gaited horses. Ultrasound images were obtained from 50 healthy adult horses of the Mangalarga Marchador (MM; n=25) and Campeiro (n=25) breeds. Static and dynamic angles of the MCP joint were measured from photographs and video recordings. Higher ME values were evinced for the SL only in the group with the smaller dynamic angles of the MCP joint in the MM horses, in addition to weak negative correlation between the dynamic angle and ME. Moreover, weak negative correlation was also observed between the static angle and TA of the lateral branch of the SL and between the static angle and the ME of the SDFT. However, the difference observed in the group of MM horses, as well as the weak correlation, was not considered sufficient to support the hypothesis that a smaller angle of the MCP joint (greater extension) is associated with larger TA and ME values for the structures. The results also suggest that the static and dynamic angles of the MCP joint do not influence the TA and ME values of the SDFT and SL in gaited horses.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7e/1f/jes-32-135.PMC8731686.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of a novel rice fermented extract on gastric ulcers in horses. 一种新型大米发酵提取物对马胃溃疡的影响。

Journal of Equine Science Pub Date : 2021-06-01 Epub Date: 2021-06-18 DOI: 10.1294/jes.32.27

Naoki Sasaki, Yasunobu Nishi, Yumi Fujiwara, Tetsuya Takeyama, Hayata Kumagai, Samantha Senarathna, Shigeto Ushiya, Takashi Tokuyama, Takahito Tokuyama, Takaaki Tokuyama, Tomohiro Mii, Satomi Ayaki, Kosuke Matsuno, Yuki Nakagawa, Yoshitaka Nishihara, Yasuho Taura

{"title":"Effect of a novel rice fermented extract on gastric ulcers in horses.","authors":"Naoki Sasaki, Yasunobu Nishi, Yumi Fujiwara, Tetsuya Takeyama, Hayata Kumagai, Samantha Senarathna, Shigeto Ushiya, Takashi Tokuyama, Takahito Tokuyama, Takaaki Tokuyama, Tomohiro Mii, Satomi Ayaki, Kosuke Matsuno, Yuki Nakagawa, Yoshitaka Nishihara, Yasuho Taura","doi":"10.1294/jes.32.27","DOIUrl":"https://doi.org/10.1294/jes.32.27","url":null,"abstract":"Gastric ulcers cause appetite loss, poor body condition, and colic in horses. This study investigated the protective effect of a rice fermented extract on the gastric mucosa in 17 healthy Thoroughbreds. For one month, horses in the rice fermented extract (nine horses) and control (eight horses) groups were orally administered a rice fermented extract (100%; 0.2 ml/kg, SID) and tap water (0.2 ml/kg), respectively. Gastric endoscopic images were obtained before and one month after rice fermented extract administration. The gastric ulcer score was lower after administration (median, 1; maximum, 2; minimum, 1) than before administration (median, 4; maximum, 4; minimum, 3) in the rice fermented extract group (P<0.05). In conclusion, the administration of a rice fermented extract for one month improves gastric mucosal lesions in Thoroughbreds with gastric ulcers.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/fe/jes-32-027.PMC8240527.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Influence of exercise and emotional stresses on secretion of prolactin and growth hormone in Thoroughbred horses. 运动和情绪应激对纯种马催乳素和生长激素分泌的影响。

Journal of Equine Science Pub Date : 2021-06-01 Epub Date: 2021-06-18 DOI: 10.1294/jes.32.49

Tomoki Kitaura, Fumio Sato, Testuro Hada, Mutsuki Ishimaru, Rumi Kodama, Yasuo Nambo, Gen Watanabe, Kazuyoshi Taya

引用次数: 3

Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads. 马妊娠期循环激活素A及其受体系统在子宫胎盘组织和胎儿性腺中的免疫定位。

Journal of Equine Science Pub Date : 2021-06-01 Epub Date: 2021-06-18 DOI: 10.1294/jes.32.39

Pramod Dhakal, Nobuo Tsunoda, Yasuo Nambo, Hiroyuki Taniyama, Kentaro Nagaoka, Gen Watanabe, Kazuyoshi Taya

{"title":"Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads.","authors":"Pramod Dhakal, Nobuo Tsunoda, Yasuo Nambo, Hiroyuki Taniyama, Kentaro Nagaoka, Gen Watanabe, Kazuyoshi Taya","doi":"10.1294/jes.32.39","DOIUrl":"https://doi.org/10.1294/jes.32.39","url":null,"abstract":"Although equine gestation is unique from the standpoint of fetal gonadal enlargement and regression, the activator of this process is still unknown. The present study aimed to show a possible role of activin during equine gestation. In the first experiment, weekly plasma samples from six pregnant mares were used to measure activin A. In the second experiment, eight pregnant mares carrying female (gestational days 110, 140, 180, and 270) and male fetuses (gestational days 120, 180, 225, and 314) were used for immunohistochemistry of activin receptors (IA, IB, IIA, IIB), and their intracellular mediators (Smad2, Smad3, Smad4). Activin A levels in maternal circulation remained low until fourth weeks of gestation, thereafter, started to increase, and peaked first at 11 weeks of gestation. The second significant peak was observed on the day of parturition. Activin receptors type IA, IB, IIA, and IIB were immunostained in interstitial and germ cells of fetal ovaries and testes along with utero-placental tissues. Smad2, Smad3, and Smad4 were also immunolocalized in all these organs. These results demonstrated the activin-producing capacity of utero-placental tissues, and also evidenced the existence of activin receptors and functional signaling molecules in these organs. The first increment in circulating activin A in maternal circulation coinciding with the timing of initiation of fetal gonadal enlargement suggests that activin from the utero-placental tissues may have a stimulatory role in fetal gonad enlargement and utero-placental development in mares, whereas the second peak could be important to follicular development in the maternal ovary for foal heat.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8a/e1/jes-32-039.PMC8240525.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Modified Z-bar shoe eliminates occasional frog bruising accompanying Z-bar shoeing for navicular syndrome management in underrun-heeled horses. 改良Z-bar鞋消除了偶尔的青蛙瘀伤伴随Z-bar鞋的舟骨综合征管理在后跟不足的马。

Journal of Equine Science Pub Date : 2021-06-01 Epub Date: 2021-06-18 DOI: 10.1294/jes.32.55

Metha Chanda, Chanikarn Puangthong, Watcharapol Pathomsakulwong, Tawanhathai Apichaimongkonkun, Juthamas Leklub

引用次数: 1