{"title":"Erratum: Genetic diversity analysis and parentage verification of Taishu horses using 31 microsatellites.","authors":"","doi":"10.1294/jes.34.19","DOIUrl":"https://doi.org/10.1294/jes.34.19","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1294/jes.33.63.].</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 1","pages":"19"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a1/22/jes-34-019.PMC10122985.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiroko AIDA, Jonathan H. FOREMAN, Akihiro OCHI, Yoshimasa TAKIZAWA, Takashi YAMANAKA
{"title":"A case of equine piroplasmosis in the Tokyo 2020 Olympic Games","authors":"Hiroko AIDA, Jonathan H. FOREMAN, Akihiro OCHI, Yoshimasa TAKIZAWA, Takashi YAMANAKA","doi":"10.1294/jes.32.93","DOIUrl":"https://doi.org/10.1294/jes.32.93","url":null,"abstract":"","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"191 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134988166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hidekazu Niwa, Toru Higuchi, Seiichi Fujii, Yuta Kinoshita, Eri Uchida-Fujii, Masuo Sueyoshi, Toshio Nukada, Takanori Ueno
{"title":"Prevalence of equine proliferative enteropathy in Hidaka district, Hokkaido, over five seasons.","authors":"Hidekazu Niwa, Toru Higuchi, Seiichi Fujii, Yuta Kinoshita, Eri Uchida-Fujii, Masuo Sueyoshi, Toshio Nukada, Takanori Ueno","doi":"10.1294/jes.33.71","DOIUrl":"https://doi.org/10.1294/jes.33.71","url":null,"abstract":"<p><p>Equine proliferative enteropathy (EPE) is an equine infectious disease that can lead to severe weight loss and hyperplasia of the intestinal mucosa due to infection with Lawsonia intracellularis. In this study, we investigated the prevalence of EPE in a major Thoroughbred breeding area: Hidaka district, Hokkaido, Japan. Of the 252 symptomatic horses that we tested, 192 EPE cases (76.2%), including 8 fatal cases, were confirmed from April 2015 to March 2020 by etiological and/or serological investigation. Most of the EPE cases were observed in foals (88.5%), with fewer cases in yearlings (7.3%) and adults (4.2%). Asymptomatic infection was observed in 62.9% of the horses kept with affected horses. These results suggest that EPE is an enzootic disease in Hidaka district.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 4","pages":"71-74"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5a/52/jes-33-071.PMC9843160.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of packing on the diurnal rhythms of respiratory and heart rates in donkeys during the hot-dry season.","authors":"Ayodele Stephen Ake, Joseph Olusegun Ayo","doi":"10.1294/jes.33.55","DOIUrl":"https://doi.org/10.1294/jes.33.55","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of packing (load carrying) on the diurnal rhythms of respiratory and heart rates in donkeys during the hot-dry season. Twenty 2- to 3-years-old donkeys weighing 93 ± 2.7 kg were divided into two groups to serve as subjects: Group 1 was subjected to packing + trekking, while group 2 was subjected to trekking only. All donkeys trekked 20 km on each experimental day. The procedure was repeated three times at one-day intervals. Thermal environmental parameters were recorded. Respiratory rate (RR) and heart rate (HR) were measured before and immediately (15 min) after the packing and/or trekking period. Continuous recordings of parameters were performed for 27 hr at 3-hr intervals beginning 16 hr after the last packing and/or trekking period. The RR rose significantly (P<0.05) immediately after packing + trekking and trekking only, from 30.15 ± 0.5 and 27.52 ± 0.5 cycles/min before packing + trekking and trekking only, respectively, to 43.78 ± 3.0 and 46.30 ± 1.8 cycles/min after them, respectively. The HR (76.63 ± 4.5 beats/min) in the packing + trekking donkeys was higher (P<0.05) than that of the trekking-only donkeys (62.33 ± 2.7 beats/min). The acrophase and bathyphase of the HR in both groups were recorded during the photophase and scotophase, respectively, with higher values (P<0.05) in the packing + trekking donkeys than in the trekking-only donkeys. The circadian rhythms of RR and HR did not differ between the groups of donkeys. In conclusion, packing + trekking was more stressful to the donkeys, significantly increasing the HR of donkeys subjected to packing + trekking, compared with trekking only.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 4","pages":"55-62"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/33/84/jes-33-055.PMC9843161.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic diversity analysis and parentage verification of Taishu horses using 31 microsatellites.","authors":"Tomoko Yoshihara, Misaki Kikuchi, Yuki Akiba, Masaharu Horiguchi, Masaki Takasu, Teruaki Tozaki","doi":"10.1294/jes.33.63","DOIUrl":"https://doi.org/10.1294/jes.33.63","url":null,"abstract":"<p><p>The Taishu horse in Tsushima is one of eight Japanese native breeds. The breed is on the verge of extinction due to a rapid decrease in numbers since the 1960s owing to motorization in Japan. In this study, we aimed to confirm the pedigree information of 52 horses by genotyping 31 microsatellites in order to avoid inbreeding. Parentage verification failed to identify genetic contradictions among trios (sires, dams, and foals) registered with the Japan Equine Affairs Association (JEAA). Pedigree information registered at the JEAA was obtained and adequately understood. Additionally, the genetic diversity of the Taishu horses was evaluated and compared with those of other Japanese native breeds. The average values for the number of alleles, observed heterozygosity, expected heterozygosity, and inbreeding coefficient were 4.7, 0.643, 0.632, and -0.02, respectively. Using the Structure software, the 52 horses were classified into three subgroups based on the individuals with more than 50% of specific genetic components. The phylogenetic trees created based on neighbor-joining classification tended to be consistent among the stallions. The effective population size was 27.5 and lower than that required for maintaining 90% genetic variation in the source population over a period of 100 years (47.5). Compared with the other Japanese breeds, the Taishu horse population included in the current study exhibited moderate genetic diversity. Our study will contribute to reconsideration of the breeding strategy of Taishu horses.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 4","pages":"63-69"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5d/22/jes-33-063.PMC9843162.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9409865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sara Azarpeykan, Erica K Gee, Keith G Thompson, Keren E Dittmer
{"title":"Undetectable vitamin D<sub>3</sub> in equine skin irradiated with ultraviolet light.","authors":"Sara Azarpeykan, Erica K Gee, Keith G Thompson, Keren E Dittmer","doi":"10.1294/jes.33.45","DOIUrl":"https://doi.org/10.1294/jes.33.45","url":null,"abstract":"<p><p>Vitamin D requirements for most animals are expected to be fulfilled through daily exposure of the skin to solar ultraviolet B radiation. The synthesis of vitamin D<sub>3</sub> in skin depends on different factors including melanin pigmentation, the amount of UVB radiation reaching the skin, type of clothing/hair coat, latitude and altitude, season, and time of day. Alternatively vitamin D<sub>2</sub> may be obtained from UVB irradiated pasture species. Recent studies have shown that in unsupplemented grazing horses 25-hydroxyvitamin D<sub>2</sub> is the predominant form of vitamin D in plasma, and that 25OHD<sub>3</sub> is undetectable suggesting horses may rely on diet to obtain vitamin D. In order to mimic the natural environment of skin to sunlight exposure, five equine and two ovine devitalized skin samples were irradiated with 5 J/cm<sup>2</sup> of UVB light followed by measurement of 7-dehydrocholesterol (7-DHC) and vitamin D<sub>3</sub> concentrations using reverse-phase high pressure liquid chromatography (HPLC). HPLC revealed the presence of 7-DHC in the skin of both horses and sheep. Vitamin D<sub>3</sub> was undetectable in both ovine and equine skin prior to irradiation, but after irradiation with UVB light, ovine skin showed an increase in vitamin D<sub>3</sub> concentration (mean 0.16 ± 0.07 µg/g), whereas vitamin D<sub>3</sub> was undetectable in equine skin. These results provide additional evidence that horses make negligible quantities of vitamin D<sub>3</sub> in their skin after exposure to UVB light and may therefore rely on their diet as a primary source of vitamin D.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 3","pages":"45-49"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/da/26/jes-33-045.PMC9522623.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33486986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monique Gonçalves Dos Santos Novelino Ramos, Sabrina Destri Emmerick Campos, Marcelo Abrahão Strauch, Leila Cardozo Ott, Daniel de Barros Macieira, Nayro Xavier de Alencar, Daniel Augusto Barroso Lessa
{"title":"Serum proteins and electrophoretic profile in horses undergoing crotalid venom hyperimmunization.","authors":"Monique Gonçalves Dos Santos Novelino Ramos, Sabrina Destri Emmerick Campos, Marcelo Abrahão Strauch, Leila Cardozo Ott, Daniel de Barros Macieira, Nayro Xavier de Alencar, Daniel Augusto Barroso Lessa","doi":"10.1294/jes.33.31","DOIUrl":"https://doi.org/10.1294/jes.33.31","url":null,"abstract":"<p><p>Indisputably, the use of antivenoms for the treatment of snakebite envenoming is beneficial for the victims. However, there are few studies addressing the effect of long-term hyperimmunization in inoculated horses. It is known that the injection of snake venoms and adjuvants leads to local and systemic reactions in horses, but little is known about the response of inflammatory proteins. The aim of this study was to evaluate serum proteins and the electrophoretic profile of horses undergoing crotalid venom hyperimmunization. Twenty horses were divided into two groups: an inoculated group, comprising ten horses that were already being used for production of a Crotalus sp. antivenom, and a control group, comprising ten animals that had never been used for hyperimmunization. All animals were clinically healthy and without laboratory abnormalities. Total protein and albumin concentrations were measured in serum. Serum globulins were obtained by calculation. Plasma fibrinogen estimates were determined by the heat precipitation method. Serum proteinograms were obtained using agarose gel electrophoresis. The results revealed a significant increase in the concentrations of total serum proteins, globulins, and β-globulins in the inoculated group, exceeding the reference values. There were slight increases in the α-1- and α-2-globulin subfractions in serum-producing horses, with no statistical significance. We also observed that horses used to produce hyperimmune plasma developed hypoalbuminemia, although the decrease in albumin production was not statistically significant. Our findings suggest that the continuous use of horses to produce crotalid antivenom may lead to a chronic inflammatory stimulus, with changes in plasma levels of inflammatory proteins.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 3","pages":"31-35"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3a/74/jes-33-031.PMC9522620.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33486988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Concentration of cephalothin in body fluids and tissue samples of Thoroughbred horses.","authors":"Taisuke Kuroda, Yohei Minamijima, Hidekazu Niwa, Hiroshi Mita, Norihisa Tamura, Kentaro Fukuda, Atsutoshi Kuwano, Fumio Sato","doi":"10.1294/jes.33.51","DOIUrl":"https://doi.org/10.1294/jes.33.51","url":null,"abstract":"<p><p>Cephalothin (CET) concentrations in body fluids (plasma, synovial fluid, pleural fluid, peritoneal fluid, and aqueous humor) and tissue samples (bone, lung, jejunum, hoof, and subcutaneous tissue) were investigated to consider the treatment of infectious diseases in horses. CET 22 mg/kg body weight was intravenously administered to 12 horses. Samples were collected from four different horses at 1, 3, and 5 hr after administration. The CET concentration in body fluids other than aqueous humor was maintained above the MIC<sub>90</sub> values of Streptococcus zooepidemicus and Staphylococcus aureus until 5 hr, but it was not maintained above that of S. aureus in bone. CET (22 mg/kg twice a day) is effective for septic arthritis, pleuritis, and peritonitis caused by gram-positive bacteria but ineffective for osteomyelitis.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 3","pages":"51-54"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/55/jes-33-051.PMC9522621.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33486987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agnieszka Pomorska-Zniszczyńska, Marcin Szczepanik, Nikola Adamczyk, Magdalena Tarach
{"title":"Videodermoscopic examination of the skin and its products in purebred Arabian horses in the summer season.","authors":"Agnieszka Pomorska-Zniszczyńska, Marcin Szczepanik, Nikola Adamczyk, Magdalena Tarach","doi":"10.1294/jes.33.37","DOIUrl":"https://doi.org/10.1294/jes.33.37","url":null,"abstract":"Videodermoscopy is a method that enables the examination of many parameters of the skin and its structures. The aim of this study was to assess specific dermoscopic parameters in purebred Arabian horses during the summer. The study involved 21 clinically healthy purebred Arabian horses (18 mares and three stallions) that had not been used for breeding and were 1 to 25 years old. The videoderoscopic evaluation was performed on seven selected areas of the body: forehead, mane, neck, chest, flank, rump, and tail. The tests were carried out with Vidix and Olympus cellSens specialised software. Videodermoscopy revealed that the skin was pigmented in all of the bay horses, in one of the seal brown horses, and in the 10 grey horses. Only one grey horse and one chestnut horse had unpigmented skin. Hair thickness ranged from 44.82 µm (neck) to 75 µm (mane). Regarding the amount of hair in the field of view, the highest and lowest numbers of hairs were found on the neck (3,004) and mane (990), respectively. A valuable insight obtained from our research is that it is possible to use digital image analysis for precise quantitative and qualitative evaluations of the skin and its structures.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 3","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/56/jes-33-037.PMC9522622.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33486989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationships between the age and blood test results or body sizes in Noma horses.","authors":"Yoichi Inoue, Tetsushi Ono, Keiichi Hisaeda, Yutaka Yamada, Akihisa Hata, Takako Shimokawa, Miyama, Kenichi Shibano, Emi Ohzawa, Hitoshi Kitagawa, Eri Iwata","doi":"10.1294/jes.33.27","DOIUrl":"https://doi.org/10.1294/jes.33.27","url":null,"abstract":"<p><p>The objective of this study is to analyze the relationships between the age and blood test results or body sizes in Noma horses by using the results of periodical health examination. Out of 45 hematological or physical items examined, statistically significant, but loose correlations were observed in 14 items. Red blood cell count, activities of aspartate aminotransferase, alkaline phosphatase, and creatinine kinase, concentrations of calcium and inorganic phosphorus decreased with aging. Conversely, mean corpuscular volume, mean corpuscular hemoglobin, lipase activity, γ-globulin and chloride concentrations, body height, chest circumference and cannon bone circumference increased with aging. The changes in a few items seemed unique to Noma horse. However, most age-related changes found in this study might be considered as a common trend in horse breeds rather than distinctive characteristic in Noma horse.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 2","pages":"27-30"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b5/d3/jes-33-027.PMC9260032.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40603282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}