Journal of Equine Science最新文献_第5页

Concentration of cephalothin in body fluids and tissue samples of Thoroughbred horses. 纯种马体液及组织样本中头孢菌素的浓度。

Journal of Equine Science Pub Date : 2022-09-01 Epub Date: 2022-09-21 DOI: 10.1294/jes.33.51

Taisuke Kuroda, Yohei Minamijima, Hidekazu Niwa, Hiroshi Mita, Norihisa Tamura, Kentaro Fukuda, Atsutoshi Kuwano, Fumio Sato

引用次数: 1

Videodermoscopic examination of the skin and its products in purebred Arabian horses in the summer season. 夏季纯种阿拉伯马皮肤及其制品的电视皮肤镜检查。

Journal of Equine Science Pub Date : 2022-09-01 Epub Date: 2022-09-21 DOI: 10.1294/jes.33.37

Agnieszka Pomorska-Zniszczyńska, Marcin Szczepanik, Nikola Adamczyk, Magdalena Tarach

{"title":"Videodermoscopic examination of the skin and its products in purebred Arabian horses in the summer season.","authors":"Agnieszka Pomorska-Zniszczyńska, Marcin Szczepanik, Nikola Adamczyk, Magdalena Tarach","doi":"10.1294/jes.33.37","DOIUrl":"https://doi.org/10.1294/jes.33.37","url":null,"abstract":"Videodermoscopy is a method that enables the examination of many parameters of the skin and its structures. The aim of this study was to assess specific dermoscopic parameters in purebred Arabian horses during the summer. The study involved 21 clinically healthy purebred Arabian horses (18 mares and three stallions) that had not been used for breeding and were 1 to 25 years old. The videoderoscopic evaluation was performed on seven selected areas of the body: forehead, mane, neck, chest, flank, rump, and tail. The tests were carried out with Vidix and Olympus cellSens specialised software. Videodermoscopy revealed that the skin was pigmented in all of the bay horses, in one of the seal brown horses, and in the 10 grey horses. Only one grey horse and one chestnut horse had unpigmented skin. Hair thickness ranged from 44.82 µm (neck) to 75 µm (mane). Regarding the amount of hair in the field of view, the highest and lowest numbers of hairs were found on the neck (3,004) and mane (990), respectively. A valuable insight obtained from our research is that it is possible to use digital image analysis for precise quantitative and qualitative evaluations of the skin and its structures.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 3","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/56/jes-33-037.PMC9522622.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33486989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationships between the age and blood test results or body sizes in Noma horses. 诺玛马的年龄与血液测试结果或体型的关系。

Journal of Equine Science Pub Date : 2022-07-01 Epub Date: 2022-07-06 DOI: 10.1294/jes.33.27

Yoichi Inoue, Tetsushi Ono, Keiichi Hisaeda, Yutaka Yamada, Akihisa Hata, Takako Shimokawa, Miyama, Kenichi Shibano, Emi Ohzawa, Hitoshi Kitagawa, Eri Iwata

引用次数: 1

The combination of BMP12 and KY02111 enhances tendon differentiation in bone marrow-derived equine mesenchymal stromal cells (BM-eMSCs). BMP12和KY02111联合使用可增强马骨髓间充质基质细胞(BM-eMSCs)的肌腱分化。

Journal of Equine Science Pub Date : 2022-07-01 Epub Date: 2022-07-06 DOI: 10.1294/jes.33.19

Aungkura Supokawej, Wasamon Korchunjit, Tuempong Wongtawan

{"title":"The combination of BMP12 and KY02111 enhances tendon differentiation in bone marrow-derived equine mesenchymal stromal cells (BM-eMSCs).","authors":"Aungkura Supokawej, Wasamon Korchunjit, Tuempong Wongtawan","doi":"10.1294/jes.33.19","DOIUrl":"https://doi.org/10.1294/jes.33.19","url":null,"abstract":"The Wingless and Int-1 (WNT) and bone morphogenic protein/growth differentiation factor (BMP/GDF) signalling pathways contribute significantly to the development of the musculoskeletal system. The mechanism by which they contribute is as follows: BMP/GDF signalling usually promotes tendon differentiation, whereas WNT signalling inhibits it. We hypothesised that inhibiting WNT and subsequently stimulating BMP signalling may enhance the tenogenic differentiation of stem cells. The objective of this study was to determine whether a combination of WNT inhibitor (KY02111) and BMP12/GDF7 protein could enhance the differentiation of bone marrow-derived equine mesenchymal stromal cells (BM-eMSCs) into tenocytes. Cells were cultured in five treatments: control, BMP12, and three different combinations of BMP12 and KY02111. The results indicated that a 1-day treatment with KY02111 followed by a 13-day treatment with BMP12 resulted in the highest tenogenic differentiation score in this experiment. The effect of KY02111 is dependent on the incubation time, with 1 day being better than 3 or 5 days. This combination increased tenogenic gene marker expression, including SCX, TNMD, DCN, and TNC, as well as COL1 protein expression. In conclusion, we propose that a combination of BMP12 and KY02111 can enhance the in vitro tenogenic differentiation of BM-eMSCs more than BMP12 alone. The findings of this study might be useful for improving tendon differentiation protocols for stem cell transplantation and application to tendon regeneration.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 2","pages":"19-26"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8f/38/jes-33-019.PMC9260033.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40603283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Epidemiological survey, general blood biochemistry, and histological examination of slaughtered heavy horse breeds with hemorrhage in the adipose tissue in the crest of the neck 屠宰的颈嵴脂肪组织出血重马品种的流行病学调查、一般血液生化和组织学检查

Journal of Equine Science Pub Date : 2022-04-01 DOI: 10.1294/jes.33.7

K. Harada, Saori Kanemitsu, Kohei Akioka, Kazunari Fujita, Y. Nishi, Y. Taura, N. Sasaki

引用次数: 1

Effect of short-term artificial light and transvaginal progesterone device on first ovulation in late transitional mares 短期人工光照和经阴道黄体酮装置对晚期母马首次排卵的影响

Journal of Equine Science Pub Date : 2022-04-01 DOI: 10.1294/jes.33.1

C. Bianchi, S. Bruno, I. Videla Dorna, Edgardo Rodríguez, M. Aba

{"title":"Effect of short-term artificial light and transvaginal progesterone device on first ovulation in late transitional mares","authors":"C. Bianchi, S. Bruno, I. Videla Dorna, Edgardo Rodríguez, M. Aba","doi":"10.1294/jes.33.1","DOIUrl":"https://doi.org/10.1294/jes.33.1","url":null,"abstract":"In study I, plasma progesterone concentrations were evaluated in anoestrous mares that received an intravaginal progesterone release device (IPRD) for 10 days. Mares were divided into 3 groups based on the dosage of progesterone (0 g, n=3; 1.38 g, n=5; and 1.9 g, n=5). No statistical differences were found in plasma progesterone concentrations between the two doses tested. In study II, the effects of a protocol based on a short program of artificial light combined with an IPRD containing 1.38 g of progesterone on oestrous behaviour and onset of ovulation were evaluated. IPRDs were inserted into 31 late transitional mares (10 days of treatment). The mares were divided into a control group (n=9, IPRD with 0 g of progesterone) and two treatment groups (T1, n=10, IPRD with 0 g of progesterone and artificial light; T2, n=12, IPRD with 1.38 g of progesterone and artificial light). The percentages of mares in heat within the first 14 days after treatment were 100%, 70%, and 100% in the control, T1, and T2 groups, respectively (P=0.097), and their ovulation rates were 44%, 60%, and 100%, respectively (P≤0.01). In conclusion, a protocol based on artificial light and an IPRD containing 1.38 g of progesterone for 10 days could be considered to advance the first ovulation of the year in late transitional mares, as it ensures a higher rate of ovulation within the first 14 days after treatment.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 1","pages":"1 - 6"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47856652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of restraint inside the transport vehicle on heart rate and heart rate variability in Thoroughbred horses 运输车内约束对纯种马心率和心率变异性的影响

Journal of Equine Science Pub Date : 2022-04-01 DOI: 10.1294/jes.33.13

H. Ohmura, A. Hiraga

引用次数: 2

Arthroscopic guided repair of a slab fracture of the fourth carpal bone in an 8-month-old thoroughbred horse. 关节镜引导下修复一匹8个月大的纯种马第四腕骨板骨折。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.143

Daisuke Miyakoshi, Akiko Watanabe, Hiroki Ikeda, Yuya Mizuguchi

引用次数: 0

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests. 基于微流控定量pcr的马基因兴奋剂检测标准物质的设计与储存稳定性。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.125

Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Kanichi Kusano, Shun-Ichi Nagata

{"title":"Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests.","authors":"Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Kanichi Kusano, Shun-Ichi Nagata","doi":"10.1294/jes.32.125","DOIUrl":"https://doi.org/10.1294/jes.32.125","url":null,"abstract":"One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"125-134"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/a1/jes-32-125.PMC8731687.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equine nonneoplastic abnormal ovary in a draft mare with high serum anti-Müllerian hormone: a case study. 马非肿瘤性卵巢异常与高血清抗<s:1>勒氏激素:一个案例研究。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.147

Akira Goto, Masaaki Tagami, Fumiki Kato, Tsukasa Suzuki, Takashi Yamaga, Harutaka Murase, Fumio Sato, Munkhtuul Tsogtgerel, Tadamasa Niikura, Tomoe Moriyama, Akiko Chiba, Ken-Ichi Watanabe, Nao Tsuzuki, Yasuo Nambo

引用次数: 2