Undetectable vitamin D3 in equine skin irradiated with ultraviolet light.

Q3 Veterinary
Journal of Equine Science Pub Date : 2022-09-01 Epub Date: 2022-09-21 DOI:10.1294/jes.33.45
Sara Azarpeykan, Erica K Gee, Keith G Thompson, Keren E Dittmer
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引用次数: 1

Abstract

Vitamin D requirements for most animals are expected to be fulfilled through daily exposure of the skin to solar ultraviolet B radiation. The synthesis of vitamin D3 in skin depends on different factors including melanin pigmentation, the amount of UVB radiation reaching the skin, type of clothing/hair coat, latitude and altitude, season, and time of day. Alternatively vitamin D2 may be obtained from UVB irradiated pasture species. Recent studies have shown that in unsupplemented grazing horses 25-hydroxyvitamin D2 is the predominant form of vitamin D in plasma, and that 25OHD3 is undetectable suggesting horses may rely on diet to obtain vitamin D. In order to mimic the natural environment of skin to sunlight exposure, five equine and two ovine devitalized skin samples were irradiated with 5 J/cm2 of UVB light followed by measurement of 7-dehydrocholesterol (7-DHC) and vitamin D3 concentrations using reverse-phase high pressure liquid chromatography (HPLC). HPLC revealed the presence of 7-DHC in the skin of both horses and sheep. Vitamin D3 was undetectable in both ovine and equine skin prior to irradiation, but after irradiation with UVB light, ovine skin showed an increase in vitamin D3 concentration (mean 0.16 ± 0.07 µg/g), whereas vitamin D3 was undetectable in equine skin. These results provide additional evidence that horses make negligible quantities of vitamin D3 in their skin after exposure to UVB light and may therefore rely on their diet as a primary source of vitamin D.

Abstract Image

紫外线照射的马皮肤中检测不到维生素D3。
大多数动物对维生素D的需求可以通过每天将皮肤暴露在太阳紫外线B辐射下来满足。皮肤中维生素D3的合成取决于不同的因素,包括黑色素沉着、到达皮肤的UVB辐射量、衣服/毛发的类型、纬度和海拔、季节和一天中的时间。另外,维生素D2也可以从UVB照射的牧草中获得。最近的研究表明,在未补充的放牧马中,25-羟基维生素D2是血浆中维生素D的主要形式,而25OHD3则无法检测到,这表明马可能依赖饮食获得维生素D。用5 J/cm2的UVB光照射5只马和2只羊的皮肤样品,用反相高压液相色谱法测定7-脱氢胆固醇(7-DHC)和维生素D3的浓度。高效液相色谱法显示马和羊的皮肤中都存在7-DHC。在UVB光照射前,绵羊和马皮肤中均未检测到维生素D3,但在UVB光照射后,绵羊皮肤中维生素D3浓度升高(平均0.16±0.07µg/g),而马皮肤中未检测到维生素D3。这些结果提供了额外的证据,表明马在暴露于UVB光后,皮肤中产生的维生素D3可以忽略不计,因此可能依赖于它们的饮食作为维生素D的主要来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Equine Science
Journal of Equine Science Veterinary-Equine
CiteScore
1.60
自引率
0.00%
发文量
9
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