{"title":"Effect of prostaglandin F2<sub>α</sub> administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses.","authors":"Tadamasa Niikura, Munkhtuul Tsogtgerel, Chizuru Niikura, Haruna Wada, Seiya Kanzawa, Takashi Fujiwara, Yasuo Nambo","doi":"10.1294/jes.32.117","DOIUrl":"https://doi.org/10.1294/jes.32.117","url":null,"abstract":"<p><p>The objective of this study was to examine the effect of prostaglandin F2<sub>α</sub> (dinoprost) and oxytocin administration on uterine polymorphonuclear neutrophil counts in the Japanese heavy draft mare. To compare polymorphonuclear neutrophil (PMN) counts in the endometrium, a total of 162 samples were collected from 54 estruses of 47 mares (before ovulation, day 0, and day 2) using a double-guarded cytology brush. Dinoprost (PG; 5 mg) was administered intramuscularly (i.m.) only once, on day 0, whereas oxytocin (OT; 20 U i.m.) was administered three times at 12-hr intervals starting on day 0. The plasma progesterone concentrations from days 0 to 14 were not different between the non-treatment (CON), PG treatment, and OT treatment groups. The PMN counts increased in all the groups from before administration to day 0 (CON, 0.90 to 3.55; PG, 1.20 to 8.45; and OT, 0.70 to 1.70; P=0.0014, 0.0046, and 0.0073, respectively). There was a significant decrease in PMNs from day 0 to day 2 only in the PG group (P=0.0073). The pregnancy rate was not different among the CON (12/18), PG (14/18), and OT (10/18) groups. The results of this study indicate that dinoprost can reduce uterine polymorphonuclear neutrophil counts.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"117-124"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/73/jes-32-117.PMC8731690.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serum cortisol in mules intended for agricultural work.","authors":"Lady C Calixto, José R Martinez","doi":"10.1294/jes.32.153","DOIUrl":"https://doi.org/10.1294/jes.32.153","url":null,"abstract":"<p><p>Cortisol is a hormone secreted by the adrenal glands that is stimulated by physiological and pathological factors and has been studied widely in equids, but not in mules. The objectives of this study were to obtain a reference value for serum cortisol in mules destined for agricultural activities and to identify age- and gender-related differences in the value. The concentration was obtained in blood samples using a commercial sandwich ELISA specific for cortisol. The concentration was 96.3 ± 40.6 ng/ml and similar between age groups and genders.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"153-155"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/af/d2/jes-32-153.PMC8731688.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39693222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jessica P Johnson, Tatiana Vinardell, Florent David
{"title":"Ultrasound-guided injections of the equine head and neck: review and expert opinion.","authors":"Jessica P Johnson, Tatiana Vinardell, Florent David","doi":"10.1294/jes.32.103","DOIUrl":"10.1294/jes.32.103","url":null,"abstract":"<p><p>Ultrasound-guided injections can be used for a wide variety of conditions in the horse, including both diagnostic and therapeutic applications. Benefits of ultrasound guidance include more accurate deposition of injectate compared with blind approaches. Improved identification of vital structures, including nerves and blood vessels, allows their avoidance and thus reduces procedure-associated complications. Validation of such ultrasound-guided techniques has shown that they can be easily learnt by inexperienced veterinarians, assuming a proper knowledge of the sonographic anatomy. In many cases they can be employed in the field with a high level of accuracy, using widely available equipment, and with complete adherence to the sterility principles. Many ultrasound-guided injection techniques of the axial skeleton in the horse have been described in past years, enabling the equine veterinarian to perform more accurate treatments of specific anatomical areas. The goal of this review is to discuss diagnostic and therapeutic ultrasound-guided injection techniques of the skull and cervical spine in the horse, including those for the retrobulbar space, maxillary and inferior alveolar nerves, atlanto-occipital and atlanto-axial junctions, and cervical articular process joints, as well as the 1st cervical nerve, the C2 and C3 nerve plexus, and the 6th, 7th, and 8th cervical nerve roots.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"103-115"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/9c/jes-32-103.PMC8731684.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jackson Schade, Anderson Fernando de Souza, Lorenzo Costa Vincensi, Joandes Henrique Fonteque
{"title":"The influence of the metacarpophalangeal joint angle on the transversal area and mean echogenicity of the superficial digital flexor tendon and suspensory ligament in gaited horses.","authors":"Jackson Schade, Anderson Fernando de Souza, Lorenzo Costa Vincensi, Joandes Henrique Fonteque","doi":"10.1294/jes.32.135","DOIUrl":"https://doi.org/10.1294/jes.32.135","url":null,"abstract":"<p><p>The objective of this study was to assess the influence of the metacarpophalangeal (MCP) joint angle on the transversal area (TA) and mean echogenicity (ME) of the superficial digital flexor tendon (SDFT) and suspensory ligament (SL) in gaited horses. Ultrasound images were obtained from 50 healthy adult horses of the Mangalarga Marchador (MM; n=25) and Campeiro (n=25) breeds. Static and dynamic angles of the MCP joint were measured from photographs and video recordings. Higher ME values were evinced for the SL only in the group with the smaller dynamic angles of the MCP joint in the MM horses, in addition to weak negative correlation between the dynamic angle and ME. Moreover, weak negative correlation was also observed between the static angle and TA of the lateral branch of the SL and between the static angle and the ME of the SDFT. However, the difference observed in the group of MM horses, as well as the weak correlation, was not considered sufficient to support the hypothesis that a smaller angle of the MCP joint (greater extension) is associated with larger TA and ME values for the structures. The results also suggest that the static and dynamic angles of the MCP joint do not influence the TA and ME values of the SDFT and SL in gaited horses.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"135-141"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7e/1f/jes-32-135.PMC8731686.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of a novel rice fermented extract on gastric ulcers in horses.","authors":"Naoki Sasaki, Yasunobu Nishi, Yumi Fujiwara, Tetsuya Takeyama, Hayata Kumagai, Samantha Senarathna, Shigeto Ushiya, Takashi Tokuyama, Takahito Tokuyama, Takaaki Tokuyama, Tomohiro Mii, Satomi Ayaki, Kosuke Matsuno, Yuki Nakagawa, Yoshitaka Nishihara, Yasuho Taura","doi":"10.1294/jes.32.27","DOIUrl":"https://doi.org/10.1294/jes.32.27","url":null,"abstract":"<p><p>Gastric ulcers cause appetite loss, poor body condition, and colic in horses. This study investigated the protective effect of a rice fermented extract on the gastric mucosa in 17 healthy Thoroughbreds. For one month, horses in the rice fermented extract (nine horses) and control (eight horses) groups were orally administered a rice fermented extract (100%; 0.2 ml/kg, SID) and tap water (0.2 ml/kg), respectively. Gastric endoscopic images were obtained before and one month after rice fermented extract administration. The gastric ulcer score was lower after administration (median, 1; maximum, 2; minimum, 1) than before administration (median, 4; maximum, 4; minimum, 3) in the rice fermented extract group (P<0.05). In conclusion, the administration of a rice fermented extract for one month improves gastric mucosal lesions in Thoroughbreds with gastric ulcers.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 2","pages":"27-30"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/fe/jes-32-027.PMC8240527.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of exercise and emotional stresses on secretion of prolactin and growth hormone in Thoroughbred horses.","authors":"Tomoki Kitaura, Fumio Sato, Testuro Hada, Mutsuki Ishimaru, Rumi Kodama, Yasuo Nambo, Gen Watanabe, Kazuyoshi Taya","doi":"10.1294/jes.32.49","DOIUrl":"https://doi.org/10.1294/jes.32.49","url":null,"abstract":"<p><p>The secretion of prolactin and growth hormone in response to exercise and emotional stresses was investigated in Thoroughbreds. Two experiments were performed: one with loading of only exercise stress and one with simultaneous loading of exercise and emotional stresses. Exercise stress was loaded in 4 steps using a treadmill for horses: pre-exercise period (5 min), walking period (6.5 min), galloping period (3 min), and cooling down period (10 min). Emotional stress was loaded by showing a loud video of an audience at a racetrack during the walking period. The results clearly demonstrated that exercise stress rapidly increased the secretion of prolactin and growth hormone and that secretion of them persisted for a specific period after the exercise. In addition, emotional stress promoted prolactin secretion.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 2","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/9f/jes-32-049.PMC8240521.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads.","authors":"Pramod Dhakal, Nobuo Tsunoda, Yasuo Nambo, Hiroyuki Taniyama, Kentaro Nagaoka, Gen Watanabe, Kazuyoshi Taya","doi":"10.1294/jes.32.39","DOIUrl":"https://doi.org/10.1294/jes.32.39","url":null,"abstract":"<p><p>Although equine gestation is unique from the standpoint of fetal gonadal enlargement and regression, the activator of this process is still unknown. The present study aimed to show a possible role of activin during equine gestation. In the first experiment, weekly plasma samples from six pregnant mares were used to measure activin A. In the second experiment, eight pregnant mares carrying female (gestational days 110, 140, 180, and 270) and male fetuses (gestational days 120, 180, 225, and 314) were used for immunohistochemistry of activin receptors (IA, IB, IIA, IIB), and their intracellular mediators (Smad2, Smad3, Smad4). Activin A levels in maternal circulation remained low until fourth weeks of gestation, thereafter, started to increase, and peaked first at 11 weeks of gestation. The second significant peak was observed on the day of parturition. Activin receptors type IA, IB, IIA, and IIB were immunostained in interstitial and germ cells of fetal ovaries and testes along with utero-placental tissues. Smad2, Smad3, and Smad4 were also immunolocalized in all these organs. These results demonstrated the activin-producing capacity of utero-placental tissues, and also evidenced the existence of activin receptors and functional signaling molecules in these organs. The first increment in circulating activin A in maternal circulation coinciding with the timing of initiation of fetal gonadal enlargement suggests that activin from the utero-placental tissues may have a stimulatory role in fetal gonad enlargement and utero-placental development in mares, whereas the second peak could be important to follicular development in the maternal ovary for foal heat.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 2","pages":"39-48"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8a/e1/jes-32-039.PMC8240525.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modified Z-bar shoe eliminates occasional frog bruising accompanying Z-bar shoeing for navicular syndrome management in underrun-heeled horses.","authors":"Metha Chanda, Chanikarn Puangthong, Watcharapol Pathomsakulwong, Tawanhathai Apichaimongkonkun, Juthamas Leklub","doi":"10.1294/jes.32.55","DOIUrl":"https://doi.org/10.1294/jes.32.55","url":null,"abstract":"<p><p>Two horses diagnosed with underrun heels leading to navicular syndrome were fitted with Z-bar shoes. They occasionally showed moderate lameness on the affected legs after the sixth and tenth consecutive farrier adjustments. The affected hooves were sensitive to compression of the frog and sole in their palmar regions. Modified Z-bar shoes were created and shod on the lame legs based on previous with regional anaesthesia and recent clinical examination. The lameness scores were slightly improved at the first shoeing with the modified Z-bar shoes but were markedly improved at four and eight weeks aftershoeing, without medication. The modified Z-bar shoes in this report could potentially protect the palmar structure damaged by usual Z-bar shoes and contribute to reducing pathogenic impacts deriving from underrun heels and navicular syndrome.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 2","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7c/63/jes-32-055.PMC8240522.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aesha A Othman, Murad A Hiblu, Mohamed Salah Abbassi, Yousef M Abouzeed, Mohamed O Ahmed
{"title":"Nasal colonization and antibiotic resistance patterns of <i>Staphylococcus</i> species isolated from healthy horses in Tripoli, Libya.","authors":"Aesha A Othman, Murad A Hiblu, Mohamed Salah Abbassi, Yousef M Abouzeed, Mohamed O Ahmed","doi":"10.1294/jes.32.61","DOIUrl":"https://doi.org/10.1294/jes.32.61","url":null,"abstract":"<p><p>The present study investigated the colonization rates and antimicrobial susceptibility of Staphylococcus species isolated from the nostrils of healthy horses. A nonselective laboratory approach was applied, followed by confirmation using a Phoenix automated microbiological system. Among the 92 horses included in the study, 48.9% (45/92) carried Staphylococcus species of mostly the coagulase-negative staphylococci (CoNS) type yielding 70 Staphylococcus strains. Of these strains, 37.1% (26/70; 24 CoNS and 2 coagulase-positive staphylococci; CoPS) were identified as methicillin-resistant staphylococci (MRS) expressing significant resistance to important antimicrobial classes represented mainly by subspecies of CoNS. This is the first study reporting a high prevalence of various Staphylococcus species, particularly strains of CoNS expressing multidrug resistance patterns of public health concern, colonizing healthy horses in Libya.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 2","pages":"61-65"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5b/f5/jes-32-061.PMC8240523.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeffrey A Fawcett, Hideki Innan, Takeru Tsuchiya, Fumio Sato
{"title":"Effect of advancing age on the reproductive performance of Japanese Thoroughbred broodmares.","authors":"Jeffrey A Fawcett, Hideki Innan, Takeru Tsuchiya, Fumio Sato","doi":"10.1294/jes.32.31","DOIUrl":"https://doi.org/10.1294/jes.32.31","url":null,"abstract":"<p><p>Many studies have reported that advancing age in broodmares has a negative impact on the reproductive performance of horses. However, although the ages at first and last mating vary among broodmares, it is unknown how this variation affects the correlation between age and reproductive performance in mares. Here, in order to examine the effects of the ages at first and last mating, we analyzed all recorded mating events for Thoroughbreds in Japan from 1997 to 2017. We found that the live foal birth rate of mares with a younger age at first mating indeed declined at an earlier age than those with an older age at first mating and that the number of years since the first mating also contributes to the decline in the birth rate. We also found that the live foal birth rate and mean earnings of the produced foals are much higher for mares with an older age at last mating compared with mares with a younger age at last mating. Our results should aid breeders in assessing the value of broodmares and designing breeding strategies.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 2","pages":"31-37"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4c/d9/jes-32-031.PMC8240526.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}