Journal of Equine Science最新文献_第6页

The combination of BMP12 and KY02111 enhances tendon differentiation in bone marrow-derived equine mesenchymal stromal cells (BM-eMSCs). BMP12和KY02111联合使用可增强马骨髓间充质基质细胞(BM-eMSCs)的肌腱分化。

Journal of Equine Science Pub Date : 2022-07-01 Epub Date: 2022-07-06 DOI: 10.1294/jes.33.19

Aungkura Supokawej, Wasamon Korchunjit, Tuempong Wongtawan

{"title":"The combination of BMP12 and KY02111 enhances tendon differentiation in bone marrow-derived equine mesenchymal stromal cells (BM-eMSCs).","authors":"Aungkura Supokawej, Wasamon Korchunjit, Tuempong Wongtawan","doi":"10.1294/jes.33.19","DOIUrl":"https://doi.org/10.1294/jes.33.19","url":null,"abstract":"The Wingless and Int-1 (WNT) and bone morphogenic protein/growth differentiation factor (BMP/GDF) signalling pathways contribute significantly to the development of the musculoskeletal system. The mechanism by which they contribute is as follows: BMP/GDF signalling usually promotes tendon differentiation, whereas WNT signalling inhibits it. We hypothesised that inhibiting WNT and subsequently stimulating BMP signalling may enhance the tenogenic differentiation of stem cells. The objective of this study was to determine whether a combination of WNT inhibitor (KY02111) and BMP12/GDF7 protein could enhance the differentiation of bone marrow-derived equine mesenchymal stromal cells (BM-eMSCs) into tenocytes. Cells were cultured in five treatments: control, BMP12, and three different combinations of BMP12 and KY02111. The results indicated that a 1-day treatment with KY02111 followed by a 13-day treatment with BMP12 resulted in the highest tenogenic differentiation score in this experiment. The effect of KY02111 is dependent on the incubation time, with 1 day being better than 3 or 5 days. This combination increased tenogenic gene marker expression, including SCX, TNMD, DCN, and TNC, as well as COL1 protein expression. In conclusion, we propose that a combination of BMP12 and KY02111 can enhance the in vitro tenogenic differentiation of BM-eMSCs more than BMP12 alone. The findings of this study might be useful for improving tendon differentiation protocols for stem cell transplantation and application to tendon regeneration.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 2","pages":"19-26"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8f/38/jes-33-019.PMC9260033.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40603283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Epidemiological survey, general blood biochemistry, and histological examination of slaughtered heavy horse breeds with hemorrhage in the adipose tissue in the crest of the neck 屠宰的颈嵴脂肪组织出血重马品种的流行病学调查、一般血液生化和组织学检查

Journal of Equine Science Pub Date : 2022-04-01 DOI: 10.1294/jes.33.7

K. Harada, Saori Kanemitsu, Kohei Akioka, Kazunari Fujita, Y. Nishi, Y. Taura, N. Sasaki

引用次数: 1

Effect of short-term artificial light and transvaginal progesterone device on first ovulation in late transitional mares 短期人工光照和经阴道黄体酮装置对晚期母马首次排卵的影响

Journal of Equine Science Pub Date : 2022-04-01 DOI: 10.1294/jes.33.1

C. Bianchi, S. Bruno, I. Videla Dorna, Edgardo Rodríguez, M. Aba

{"title":"Effect of short-term artificial light and transvaginal progesterone device on first ovulation in late transitional mares","authors":"C. Bianchi, S. Bruno, I. Videla Dorna, Edgardo Rodríguez, M. Aba","doi":"10.1294/jes.33.1","DOIUrl":"https://doi.org/10.1294/jes.33.1","url":null,"abstract":"In study I, plasma progesterone concentrations were evaluated in anoestrous mares that received an intravaginal progesterone release device (IPRD) for 10 days. Mares were divided into 3 groups based on the dosage of progesterone (0 g, n=3; 1.38 g, n=5; and 1.9 g, n=5). No statistical differences were found in plasma progesterone concentrations between the two doses tested. In study II, the effects of a protocol based on a short program of artificial light combined with an IPRD containing 1.38 g of progesterone on oestrous behaviour and onset of ovulation were evaluated. IPRDs were inserted into 31 late transitional mares (10 days of treatment). The mares were divided into a control group (n=9, IPRD with 0 g of progesterone) and two treatment groups (T1, n=10, IPRD with 0 g of progesterone and artificial light; T2, n=12, IPRD with 1.38 g of progesterone and artificial light). The percentages of mares in heat within the first 14 days after treatment were 100%, 70%, and 100% in the control, T1, and T2 groups, respectively (P=0.097), and their ovulation rates were 44%, 60%, and 100%, respectively (P≤0.01). In conclusion, a protocol based on artificial light and an IPRD containing 1.38 g of progesterone for 10 days could be considered to advance the first ovulation of the year in late transitional mares, as it ensures a higher rate of ovulation within the first 14 days after treatment.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"33 1","pages":"1 - 6"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47856652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of restraint inside the transport vehicle on heart rate and heart rate variability in Thoroughbred horses 运输车内约束对纯种马心率和心率变异性的影响

Journal of Equine Science Pub Date : 2022-04-01 DOI: 10.1294/jes.33.13

H. Ohmura, A. Hiraga

引用次数: 2

Arthroscopic guided repair of a slab fracture of the fourth carpal bone in an 8-month-old thoroughbred horse. 关节镜引导下修复一匹8个月大的纯种马第四腕骨板骨折。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.143

Daisuke Miyakoshi, Akiko Watanabe, Hiroki Ikeda, Yuya Mizuguchi

引用次数: 0

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests. 基于微流控定量pcr的马基因兴奋剂检测标准物质的设计与储存稳定性。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.125

Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Kanichi Kusano, Shun-Ichi Nagata

{"title":"Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests.","authors":"Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Kanichi Kusano, Shun-Ichi Nagata","doi":"10.1294/jes.32.125","DOIUrl":"https://doi.org/10.1294/jes.32.125","url":null,"abstract":"One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"125-134"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/a1/jes-32-125.PMC8731687.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equine nonneoplastic abnormal ovary in a draft mare with high serum anti-Müllerian hormone: a case study. 马非肿瘤性卵巢异常与高血清抗<s:1>勒氏激素:一个案例研究。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.147

Akira Goto, Masaaki Tagami, Fumiki Kato, Tsukasa Suzuki, Takashi Yamaga, Harutaka Murase, Fumio Sato, Munkhtuul Tsogtgerel, Tadamasa Niikura, Tomoe Moriyama, Akiko Chiba, Ken-Ichi Watanabe, Nao Tsuzuki, Yasuo Nambo

引用次数: 2

Effect of prostaglandin F2_α administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses. 前列腺素F2α对日本重驮马子宫多形核中性粒细胞计数的影响。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.117

Tadamasa Niikura, Munkhtuul Tsogtgerel, Chizuru Niikura, Haruna Wada, Seiya Kanzawa, Takashi Fujiwara, Yasuo Nambo

{"title":"Effect of prostaglandin F2α administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses.","authors":"Tadamasa Niikura, Munkhtuul Tsogtgerel, Chizuru Niikura, Haruna Wada, Seiya Kanzawa, Takashi Fujiwara, Yasuo Nambo","doi":"10.1294/jes.32.117","DOIUrl":"https://doi.org/10.1294/jes.32.117","url":null,"abstract":"The objective of this study was to examine the effect of prostaglandin F2α (dinoprost) and oxytocin administration on uterine polymorphonuclear neutrophil counts in the Japanese heavy draft mare. To compare polymorphonuclear neutrophil (PMN) counts in the endometrium, a total of 162 samples were collected from 54 estruses of 47 mares (before ovulation, day 0, and day 2) using a double-guarded cytology brush. Dinoprost (PG; 5 mg) was administered intramuscularly (i.m.) only once, on day 0, whereas oxytocin (OT; 20 U i.m.) was administered three times at 12-hr intervals starting on day 0. The plasma progesterone concentrations from days 0 to 14 were not different between the non-treatment (CON), PG treatment, and OT treatment groups. The PMN counts increased in all the groups from before administration to day 0 (CON, 0.90 to 3.55; PG, 1.20 to 8.45; and OT, 0.70 to 1.70; P=0.0014, 0.0046, and 0.0073, respectively). There was a significant decrease in PMNs from day 0 to day 2 only in the PG group (P=0.0073). The pregnancy rate was not different among the CON (12/18), PG (14/18), and OT (10/18) groups. The results of this study indicate that dinoprost can reduce uterine polymorphonuclear neutrophil counts.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"117-124"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/73/jes-32-117.PMC8731690.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum cortisol in mules intended for agricultural work. 用于农业工作的骡子血清中的皮质醇。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.153

Lady C Calixto, José R Martinez

引用次数: 0

Ultrasound-guided injections of the equine head and neck: review and expert opinion. 超声波引导下的马头和颈部注射：综述和专家意见。

Journal of Equine Science Pub Date : 2021-12-01 Epub Date: 2021-12-28 DOI: 10.1294/jes.32.103

Jessica P Johnson, Tatiana Vinardell, Florent David

{"title":"Ultrasound-guided injections of the equine head and neck: review and expert opinion.","authors":"Jessica P Johnson, Tatiana Vinardell, Florent David","doi":"10.1294/jes.32.103","DOIUrl":"10.1294/jes.32.103","url":null,"abstract":"Ultrasound-guided injections can be used for a wide variety of conditions in the horse, including both diagnostic and therapeutic applications. Benefits of ultrasound guidance include more accurate deposition of injectate compared with blind approaches. Improved identification of vital structures, including nerves and blood vessels, allows their avoidance and thus reduces procedure-associated complications. Validation of such ultrasound-guided techniques has shown that they can be easily learnt by inexperienced veterinarians, assuming a proper knowledge of the sonographic anatomy. In many cases they can be employed in the field with a high level of accuracy, using widely available equipment, and with complete adherence to the sterility principles. Many ultrasound-guided injection techniques of the axial skeleton in the horse have been described in past years, enabling the equine veterinarian to perform more accurate treatments of specific anatomical areas. The goal of this review is to discuss diagnostic and therapeutic ultrasound-guided injection techniques of the skull and cervical spine in the horse, including those for the retrobulbar space, maxillary and inferior alveolar nerves, atlanto-occipital and atlanto-axial junctions, and cervical articular process joints, as well as the 1st cervical nerve, the C2 and C3 nerve plexus, and the 6th, 7th, and 8th cervical nerve roots.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 4","pages":"103-115"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/9c/jes-32-103.PMC8731684.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0