{"title":"Case report: The case of a 17 kg ovarian granulosa cell tumor in a Breton draft mare.","authors":"Munkhtuul Tsogtgerel, Masaaki Tagami, Kenichi Watanabe, Harutaka Murase, Yuko Hirosawa, Yoshiyasu Kobayashi, Yasuo Nambo","doi":"10.1294/jes.32.67","DOIUrl":"https://doi.org/10.1294/jes.32.67","url":null,"abstract":"<p><p>Granulosa cell tumor (GCT) is a benign tumor which affects the mare's ovaries. In this report, a case of unilateral GCT in an ovary, which weighed 17.04 kg, of a 9-year-old Breton draft mare is described. A transrectal ultrasonography exam revealed a unilateral multi-cystic enlarged ovary. Laparoscopic ovariectomy was difficult due to enlargement of blood vessels in the ovarian broad ligament. The mare was necropsied, and the pathological changes in the GCT-affected ovary and unaffected ovary were evaluated. The ovarian mass in the GCT-affected ovary had a cribriform pattern and was positive for anti-Müllerian hormone (AMH) and its receptor (AMHR2). The contralateral ovary showed no follicular development and was negative for AMH. AMHR2 was positively expressed in stromal cells. The AMH concentration in plasma was 4,210 ng/ml. This is the first report showing the presence of AMH (2,210 ng/ml) in ascites fluid, and it also shows that laparoscopic ovariectomy might not be suitable for larger ovaries affected by a GCT. Ultrasonographic, endocrine, and histopathological analyses were helpful for making a definitive diagnosis of GCT in this mare.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 2","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/34/fc/jes-32-067.PMC8240524.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigation of erythrocyte antigen frequencies in draft horse populations in Japan to assess blood donor suitability.","authors":"Hironaga Kakoi, Mio Kikuchi, Taichiro Ishige, Shun-Ichi Nagata, Yuko Hirosawa, Shoko Tanaka, Takumi Kishinami","doi":"10.1294/jes.32.17","DOIUrl":"https://doi.org/10.1294/jes.32.17","url":null,"abstract":"<p><p>Erythrocyte alloantigen frequencies of draft horses in Japan were investigated to assess blood donor suitability for transfusion. Here, 148 Japanese draft, 69 Percheron, and 65 Breton horses were blood-typed and subjected to an indirect antiglobulin test. Regarding the major immunogenic factors, the rates of Aa- and Qa-negative horses ranged from 0.35 to 0.49 and from 0.82 to 1.00, respectively. The rate of alloantibody-positive horses ranged from 0.12 to 0.35. Although the prevalence of alloantibodies in these horses was higher than that expected naturally, the rates of Aa- and Qa-negative horses were higher than those of some breeds reported previously. The current draft horse population could provide potential candidates for donors, and the obtained information may contribute to the selection of a safe donor for transfusion.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 1","pages":"17-19"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/e8/jes-32-017.PMC7984914.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25524132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Equine rotavirus infection.","authors":"Manabu Nemoto, Tomio Matsumura","doi":"10.1294/jes.32.1","DOIUrl":"https://doi.org/10.1294/jes.32.1","url":null,"abstract":"<p><p>This review briefly describes the virus classification, clinical signs, epidemiology, diagnosis, disinfection, and vaccines related equine group A rotavirus (RVA) infection. Equine RVA is one of the most important pathogens causing diarrhoea in foals. The main transmission route is faecal-oral, and the clinical signs are diarrhoea, fever, lethargy, and anorexia (decreased suckling). Some human RVA rapid antigen detection kits based on the principles of the immunochromatographic assay are useful for the diagnosis of equine RVA infection. The kits are used in daily clinical practice because of their rapidity and ease of handling. Equine RVA is a non-enveloped virus and is more resistant to disinfectants than enveloped viruses such as equine influenza virus and equine herpesvirus. Although amphoteric soaps and quaternary ammonium compounds are commonly used in veterinary hygiene, they are generally ineffective against equine RVA. Alcohol products, aldehydes, and chlorine- and iodine-based compounds are effective against equine RVA. Inactivated vaccines have been used for equine RVA infection in some countries. Pregnant mares are intramuscularly inoculated with a vaccine, and thus their colostrum has abundant antibodies against RVA at the time of birth. According to G and P classification defined in accordance with the VP7 and VP4 genes, respectively, the predominant equine RVAs circulating in horse populations globally are G3P[12] and G14P[12] equine RVAs, but the vaccines contain only the G3P[12] equine RVA strain. Ideally, a G14P[12] equine RVA should be added as a vaccine strain to obtain a better vaccine effect.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/47/jes-32-001.PMC7984913.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25524130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of seasons and sex on the physical, hematological, and blood biochemical parameters of Noma horses.","authors":"Tetsushi Ono, Yoichi Inoue, Keiichi Hisaeda, Yutaka Yamada, Akihisa Hata, Takako Shimokawa Miyama, Kenichi Shibano, Hitoshi Kitagawa, Emi Ohzawa, Eri Iwata","doi":"10.1294/jes.32.21","DOIUrl":"https://doi.org/10.1294/jes.32.21","url":null,"abstract":"<p><p>This study aimed to evaluate the influence of seasons and sex on body size and hematological and biochemistry parameters of Noma horses, a native Japanese breed. Body size was larger in winter than in summer. Laboratory testing variables, including erythrocytic parameters and urea nitrogen, total cholesterol, and creatinine kinase levels, were higher in winter, while the eosinophil count was higher in summer. These seasonal differences may be related to increased energy consumption of horses due to heat stress. The higher eosinophil counts may have been related to the dermatitis observed in summer. Stallions tended to have smaller bodies compared with mares. Future studies are necessary to investigate the effect of stress in seasonal and sex-based groups.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 1","pages":"21-25"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a5/68/jes-32-021.PMC7984912.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25524133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yohei Minamijima, Hidekazu Niwa, Eri Uchida, Kazuo Yamamoto
{"title":"Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics.","authors":"Yohei Minamijima, Hidekazu Niwa, Eri Uchida, Kazuo Yamamoto","doi":"10.1294/jes.32.11","DOIUrl":"https://doi.org/10.1294/jes.32.11","url":null,"abstract":"<p><p>In the past decade, mass spectrometry has become an important technology for protein identification. Recent developments in mass spectrometry allow a large number of identifications in samples; therefore, mass-spectrometry-based techniques have been applied to the discovery of biomarkers. Here, we conducted a proteomic study to compare the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport (RDT). We found that four proteins, apolipoprotein F, lipopolysaccharide binding protein, lysozyme and protein S100-A8, were upregulated, while keratin 1 was downregulated in the RDT group. It is assumed that inflammation and immune response are involved in the changes of these proteins. The findings suggested that these proteins are potentially useful for elucidating the mechanism of development of RDT.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/a3/jes-32-011.PMC7984915.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25524131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Body measurement of riding horses with a versatile tablet-type 3D scanning device.","authors":"Akihiro Matsuura, Maiko Dan, Aiko Hirano, Yoshio Kiku, Suzuka Torii, Shigeru Morita","doi":"10.1294/jes.32.73","DOIUrl":"https://doi.org/10.1294/jes.32.73","url":null,"abstract":"<p><p>The measurement of various body dimensions of horses plays a significant role in quality improvement, genetic breeding, health, and soundness. There has been significant advancement in the technology for acquiring stereoscopic images with a three-dimensional (3D) scanner. This study aimed to validate the accuracy of body measurements obtained from stereoscopic images taken with a 3D scanner. We manually took the following body measurements for 8 riding horses: height at the withers, height at the back, height at the croup, chest depth, width of the chest, width of the croup, width of the waist, girth circumference, cannon circumference, and body length. Using a versatile tablet-type 3D scanning device, we captured a 3D image of each horse. Relative errors varied from -1.37% to 6.25%. The correlation coefficient between manual and 3D measurements was significant for all body measurements (P<0.01) except for width of the waist and cannon circumference. The low accuracy of cannon circumference (r=0.248) was due to effect of hair. A simple regression analysis of all body measurements revealed a strong correlation (P<0.001, R<sup>2</sup>=0.9994, root-mean-square error [RMSE]=1.522). Notable advantages of this methodology include high accuracy, good operability, non-contact, high versatility, and low cost. Further studies are required for the establishment of an accurate measurement methodology that can scan the whole body in a shorter time.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 3","pages":"73-80"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c1/9b/jes-32-073.PMC8437753.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39429114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Equine spinal kinematics derived from different riding positions during asymmetrical bareback riding.","authors":"Nuttawut Nuchprayoon, Pattama Ritruechai, Krisana Watchararat, Weerawat Limroongruengrat, Tuempong Wongtawan, Nlin Arya","doi":"10.1294/jes.32.81","DOIUrl":"https://doi.org/10.1294/jes.32.81","url":null,"abstract":"<p><p>Hippotherapy in patients with neuromuscular dysfunction creates high focal pressure on the pony's back due to bareback riding and an asymmetrical riding position. This study aimed to investigate the acute effect of asymmetrical bareback riding on the pony's spinal kinematics, blood lactate, serum creatine kinase, heart rate, and temperament score. Eight ponies were selected, and they were walked on a treadmill for 45 min on each experimental day, including warm-up (5 min), weight-loading by mannequin (30 min), and cool-down (10 min) sessions. During the weight-loading session, three different weight distributions on the pony's back were applied between the left and right side: 50:50 (treatment M), 70:30 (treatment L), and 30:70 (treatment R) on the first, second, and third day of the experiment, respectively. The spinal kinematics at the end of the weight-loading session revealed a slight reduction in range of motion in both flexion-extension and lateral bending during treatment R. Stride length and stride duration showed no differences between treatments. The levels of blood lactate and serum creatine kinase and results of a back examination were normal. Heart rates and temperament scores revealed that all ponies were calm throughout loading of the mannequin. This information suggests that asymmetrical bareback riding did not cause acute or serious back injury, which indicates good equine welfare in ponies used for hippotherapy.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 3","pages":"81-89"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c8/57/jes-32-081.PMC8437752.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39429115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Persistence of virus-neutralizing antibodies in horses inoculated with two doses of a live equine herpesvirus type 1 vaccine with different vaccination intervals.","authors":"Hiroshi Bannai, Yoshinori Kambayashi, Koji Tsujimura, Tsuyoshi Nagashima, Naoya Takebe, Masataka Tominari, Manabu Nemoto, Minoru Ohta","doi":"10.1294/jes.32.99","DOIUrl":"https://doi.org/10.1294/jes.32.99","url":null,"abstract":"<p><p>The antibody response in horses inoculated with 2 doses of a live equine herpesvirus type 1 vaccine with different vaccination intervals (1 to 3 months) was evaluated with regard to the persistence of virus-neutralizing (VN) antibodies. The durations for which the geometric mean VN titers were maintained significantly higher than those before the first vaccination (P<0.05) were up to 5 months in horses that received the vaccination with a 1-month interval (n=17) and 7 months for those that received it with a 2-month (n=17) or 3-month interval (n=14 or 17). The vaccination program with the 2-month interval was the most effective in maintaining VN antibodies for a long duration with the smallest gap of antibody decline between the first and second vaccinations.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 3","pages":"99-102"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/e7/jes-32-099.PMC8437755.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39429117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship between lactational performance and metabolic parameters of Mongolian native grazing mares.","authors":"Badrakh Sandagdorj, Tserenpil Baigalmaa, Sedhuu Burenjargal, Motohiro Horiuchi, Munkhbat Enkhdalai, Davaakhuu Bayanbat, Dashdorj Janchiv, Ooyo Jamyandorj, Purevdorj Ulzii-Orshikh, Purevdorj Nyam-Osor","doi":"10.1294/jes.32.91","DOIUrl":"https://doi.org/10.1294/jes.32.91","url":null,"abstract":"<p><p>Information regarding the lactational performance of mares in relation to metabolic parameters can help practitioners to manipulate animal rearing management for sustainable mare milk production. The aim of this study was to characterize the lactational performance of Mongolian native mares grazing on natural pastureland by revealing the seasonal effects on metabolic parameters. In this study, 8 multiparous mares were used. Milk yield and composition and serum metabolic parameters, such as alanine aminotransferase, aspartate aminotransferase (AST), glucose (GLU), triacylglycerol, total cholesterol (TCH), non-esterified fatty acid (NEFA), albumin, urea, total protein, cortisol (Cort), and insulin, were determined at 30, 60, 90, 120, 150, 180, 210, 240, and 270 days of lactation. During the lactation period, milk yield peaked at around the 90th day and declined sharply in the following period. While the milk fat and protein contents decreased gradually from the early stages of lactation to the late stages, the lactose content was highest at mid-lactation and stayed constant until the end of the lactation period. Meanwhile, changes were observed between the stages of lactation, and the differences in metabolic parameters were significant (P<0.05), except for AST and GLU. The strongest correlation was found with NEFA (P<0.01), followed by the Cort (P<0.05) concentration, with both parameters showing negative correlation, and strong positive correlation was detected between the milk yield and TCH (P<0.05) concentration.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"32 3","pages":"91-98"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e6/42/jes-32-091.PMC8437754.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39429116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Trung B Nguyen, Ripon C Paul, Yu Okuda, Thu N A LE, Phuong T K Pham, Kushaliye J Kaissar, Akhmedenov Kazhmurat, Sarsenova Bibigul, Meirat Bakhtin, Polat Kazymbet, Suleimenov Zh Maratbek, Alikhan Meldebekov, Masahide Nishibori, Takayuki Ibi, Takehito Tsuji, Tetsuo Kunieda
{"title":"Genetic characterization of Kushum horses in Kazakhstan based on haplotypes of mtDNA and Y chromosome, and genes associated with important traits of the horses.","authors":"Trung B Nguyen, Ripon C Paul, Yu Okuda, Thu N A LE, Phuong T K Pham, Kushaliye J Kaissar, Akhmedenov Kazhmurat, Sarsenova Bibigul, Meirat Bakhtin, Polat Kazymbet, Suleimenov Zh Maratbek, Alikhan Meldebekov, Masahide Nishibori, Takayuki Ibi, Takehito Tsuji, Tetsuo Kunieda","doi":"10.1294/jes.31.35","DOIUrl":"https://doi.org/10.1294/jes.31.35","url":null,"abstract":"<p><p>The Kushum is a relatively new breed of horses in Kazakhstan that was established in the middle of the 20th century through a cross between mares of Kazakhstan local horses and stallions of Thoroughbred, Trotter, and Russian Don breeds to supply military horses. To reveal the genetic characteristics of this breed, we investigated haplotypes of mitochondrial DNA (mtDNA) and single-nucleotide polymorphisms of the Y chromosome, as well as genotypes of five functional genes associated with coat color, body composition, and locomotion traits. We detected 10 mtDNA haplotypes that fell into 8 of the 17 major haplogroups of horse mtDNA, indicating a unique haplotype composition with high genetic diversity. We also found two Y-chromosomal haplotypes in Kushum horses, which likely originated from Trotter and/or Don breeds. The findings regarding the mtDNA and Y-chromosomal haplotypes are concordant with the documented maternal and paternal origins of the Kushum horses. The allele frequencies of ASIP, MC1R, and MATP associated with coat color were consistent with the coat color variations of Kushum horses. The allele frequencies of MSTN associated with endurance performance and those of DMRT3 associated with gait suggested that the observed allele frequencies of these genes were the result of selective breeding for these traits. As a result of this study, we were able to obtain useful information for a better understanding of the origin and breeding history of the Kushum horse breed using molecular markers.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"31 3","pages":"35-43"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6e/96/jes-31-035.PMC7538259.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38494261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}