Eri Uchida-Fujii, Hidekazu Niwa, Yuta Kinoshita, Yoshinari Katayama, Toshio Nukada
{"title":"Molecular phylogenetic and matrix-assisted laser desorption ionization time-of-flight mass spectrometry identification of isolates from horses identified as <i>Enterobacter cloacae</i> by biochemical identification.","authors":"Eri Uchida-Fujii, Hidekazu Niwa, Yuta Kinoshita, Yoshinari Katayama, Toshio Nukada","doi":"10.1294/jes.31.49","DOIUrl":"10.1294/jes.31.49","url":null,"abstract":"<p><p>Enterobacter cloacae is an opportunistic pathogen of horses. Thirty isolates obtained from horses and their environments and identified as Enterobacter cloacae by biochemical methods were reidentified by taxonomic identification based on multilocus sequence analysis (MLSA) and by a commercial identification system based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). MLSA identified the 30 equine isolates as E. ludwigii (9/30), E. asburiae (1/30), or E. cloacae (1/30); 19 isolates were not identified. The MALDI-TOF MS system could not clearly distinguish isolates to the species level, and the limited numbers of reference spectra for Enterobacter species might have contributed to the poor identification.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"31 3","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/97/7f/jes-31-049.PMC7538261.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38492620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Everest O Atadiose, Junaidu Kabir, Shuaibu G Adamu, Jarlath U Umoh
{"title":"Serosurvey of West Nile virus in horses and detection of West Nile virus antigen in mosquitoes in Kaduna State, Nigeria.","authors":"Everest O Atadiose, Junaidu Kabir, Shuaibu G Adamu, Jarlath U Umoh","doi":"10.1294/jes.31.61","DOIUrl":"https://doi.org/10.1294/jes.31.61","url":null,"abstract":"<p><p>West Nile virus (WNV) causes a mosquito-borne zoonotic disease of public health importance. The aim of this study was to determine the state of WNV infection in horses and detect the virus antigen in mosquitoes trapped in stables in Kaduna State Nigeria. The study was carried out in Kaduna State, Nigeria, and 368 horses were screened for the presence of antibodies against WNV using an IgG competitive enzyme-linked immunosorbent assay. Of the 368 samples tested, 331 (89.9%) were positive for WNV antibodies. Mosquitoes from the stables were tested for WNV antigen using a VectorTest kit, and of the 31 pools of adult mosquitoes tested, only 5 (16.1%) pools tested positive for WNV antigen. This finding showed that WNV infection has occurred in horses and that there is evidence of circulation of the virus by mosquitoes in Kaduna State, Nigeria.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"31 3","pages":"61-66"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/66/89/jes-31-061.PMC7538258.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38492622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ivana Louise Santos Silva, Glebb Strauss Borges Junqueira, Chiara Albano de Araújo Oliveira, Raphael Bermal Costa, Gregório Miguel Ferreira DE Camargo
{"title":"Inconsistencies in horse coat color registration: A case study.","authors":"Ivana Louise Santos Silva, Glebb Strauss Borges Junqueira, Chiara Albano de Araújo Oliveira, Raphael Bermal Costa, Gregório Miguel Ferreira DE Camargo","doi":"10.1294/jes.31.57","DOIUrl":"https://doi.org/10.1294/jes.31.57","url":null,"abstract":"<p><p>Grullo is a dun dilution on a black coat that is common in the Campolina horse: an autochthonous Brazilian breed. The aims of this case study were to evaluate inconsistencies in grullo coat color registration and to explain their possible causes. A total of 3,270 grullo Campolina horses were evaluated. To confirm the genetic possibility of having grullo animals, the coat color genotypes of parents were inferred by phenotype and compared with those of progeny. A total of 242 horses that were registered as grullos could not have this coat based on their parents' information. Possible explanations for incorrect registration are errors of paternity and in coat color identification. We suggest maintaining obligatory paternity testing and enhancing training in coat color identification.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"31 3","pages":"57-60"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/8a/jes-31-057.PMC7538257.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38492621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Riccardo Rinnovati, Socrate Xenos, Andrea Beltrame, Andrea Marigo, Giulia Forni, Maria Morini
{"title":"Subcutaneous neurofibroma as a cause of lameness in a warmblood horse: Neurofibroma in a horse.","authors":"Riccardo Rinnovati, Socrate Xenos, Andrea Beltrame, Andrea Marigo, Giulia Forni, Maria Morini","doi":"10.1294/jes.31.45","DOIUrl":"https://doi.org/10.1294/jes.31.45","url":null,"abstract":"<p><p>A neurofibroma was excised from the subcutis on the medial side of the left thigh of a 15-year-old Warmblood gelding, which had shown lameness of the left hind limb. No other source of lameness was found. Two weeks after surgery, the horse was sound at a lameness examination.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"31 3","pages":"45-48"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/4b/jes-31-045.PMC7538260.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38492619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Equine Sarcoid","authors":"Beatrice Funiciello, P. Roccabianca","doi":"10.5772/intechopen.91013","DOIUrl":"https://doi.org/10.5772/intechopen.91013","url":null,"abstract":"","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78856350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Equine Reproduction: Seasonality, Endometritis, and Twinning in the Mare","authors":"D. Trundell","doi":"10.5772/intechopen.92999","DOIUrl":"https://doi.org/10.5772/intechopen.92999","url":null,"abstract":"This chapter reviews the seasonality and effect of photoperiod in mares and how, as clinicians, we can shorten the vernal transition period and improve our efficiency in getting mares in foal. Different protocols have been utilized to shorten the vernal transition, and each will be discussed. We will also examine endometritis in the mare. The role of biofilms in causing endometritis in our equine patients, and potential treatment plans, in particular breeding the dirty mare, will be reviewed. Finally, we will examine the effect of twin pregnancies in the mare, the most common cause of noninfectious abortion, and offer two management therapies for dealing with twinning in the mare.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83858699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ADAMTS Proteases: Potential Biomarkers and Novel Therapeutic Targets for Cartilage Health","authors":"S. Kandir","doi":"10.5772/intechopen.93046","DOIUrl":"https://doi.org/10.5772/intechopen.93046","url":null,"abstract":"The equine locomotor system’s health plays a key role on athletic performance. Bone and joint diseases are the major causes of lameness. Poor performance and diseases lead to great economic loss to equestrian sports and horse breeders. Therefore, prevention, early diagnosis, and therapy of joint diseases are important. A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteinase family plays an important role in many physiological processes such as tissue reorganization, coagulation, and angiogenesis. Aggrecan proteinases ADAMTS-4 and ADAMTS-5 are physiologically responsible for the restructuring with enzymatic cleavage of the cartilage, specific biomarkers in the synovium or body fluids for early diagnosis, and potential specific therapeutic targets in order to their role on degenerative joint diseases physiopathology in humans and various animals.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"185 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74080195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Zakirova, Kovac Milomir, M. Zhuravleva, Catrin Rutland, A. Rizvanov
{"title":"Gene Therapy as a Modern Method of Treating Naturally Occurring Tendinitis and Desmitis in Horses","authors":"E. Zakirova, Kovac Milomir, M. Zhuravleva, Catrin Rutland, A. Rizvanov","doi":"10.5772/intechopen.92352","DOIUrl":"https://doi.org/10.5772/intechopen.92352","url":null,"abstract":"Tendon and ligament injuries have always been complex to treat, with recovery often taking many months, if successful at all. This chapter looks at recent work undertaken using regenerative medicine, specifically gene therapy and the advances that have been made in equine therapy. It looks at the process from plasmid construction, in vitro testing through to trialing the equine-specific plasmid construct in horses with superficial digital flexor tendon (tendinitis) and suspensory ligament branch injuries. It also looks at the rationale for utilizing vascular endothelial growth factor (VEGF164) and a basic fibroblast growth factor (FGF2) for these trials and the cellular effects and potential mechanisms of actions.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"145 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77103271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. W. Levings, Andrew D. Smith, P. Levings, G. Palmer, Anthony Dacanay, P. Colahan, S. Ghivizzani
{"title":"Gene Therapy for the Treatment of Equine Osteoarthritis","authors":"R. W. Levings, Andrew D. Smith, P. Levings, G. Palmer, Anthony Dacanay, P. Colahan, S. Ghivizzani","doi":"10.5772/intechopen.93000","DOIUrl":"https://doi.org/10.5772/intechopen.93000","url":null,"abstract":"Osteoarthritis (OA) is the predominant cause of lameness in horses. As in humans, the clinical symptoms of equine OA are persistent pain and dysfunction of the affected joint. Its pathology is similarly marked by progressive deterioration of the articular cartilage, subchondral bone sclerosis, marginal osteophytes, soft tissue inflammation and joint effusion. Disease pathogenesis is mediated by elevated levels of inflammatory cytokines and proteolytic enzymes in the articular tissues and synovial fluid. Existing pharmacologic agents can alleviate OA joint pain; none are able to inhibit erosive disease progression. As several gene-based treatments for human disease have received approval by the Food and Drug Administration (FDA), the transition to veterinary medicine will almost certainly follow. Several viral vector systems have demonstrated highly efficient gene transfer to the equine joint, enabling expression of therapeutic transgenes at efficacious levels for well over a year. Because of its large size, the equine joint is well suited to studies of gene-based therapies for arthritic disease. The forelimb joints are vulnerable to OA onset, and treatment and diagnostic modalities are the same in humans and horses. Here, we discuss the various gene-transfer approaches under investigation and the current progress toward the development an effective gene therapy for equine OA.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89820153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Colostral and foal serum immunoglobulin G levels and associations with perinatal abnormalities in heavy draft horses in Japan.","authors":"Takahiro Aoki, Akiko Chiba, Megumi Itoh, Yasuo Nambo, Norio Yamagishi, Ken-Ichi Shibano, Soon Hon Cheong","doi":"10.1294/jes.31.29","DOIUrl":"https://doi.org/10.1294/jes.31.29","url":null,"abstract":"<p><p>The purpose of this study was to elucidate the colostral and foal serum immunoglobulin G (IgG) concentration values in heavy draft horses in Japan and to examine the effects of peripartum mare condition on colostral immunity. Colostrum was obtained 1 hr after foaling (pre-suckling; n=178). Blood was collected from the jugular vein of the foals (n=147) at 24 to 48 hr after birth. The foaling statuses of 73 mares were recorded. The average colostral IgG concentration was 10,540 ± 3,190 mg/dl (median=10,928; range 1,434-17,514 mg/dl). The average serum IgG concentration obtained from neonatal foals 24 to 48 hr after birth was 1,750 ± 919 mg/dl (median=1,890; range 0-3,510 mg/dl). Although colostral IgG did not differ between the normal foaling mare (n=59) and dystocial mare (n=14), foal serum IgG was lower in foals born in dystocia than in foals in normal foaling (P<0.05). This study demonstrates reference values for colostral and foal serum IgG specific to heavy draft horses in Japan and suggests that dystocia may interfere with the acquisition of colostral immunity in neonatal foals.</p>","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"31 2","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b8/e4/jes-31-029.PMC7316699.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38118159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}