Serum proteins and electrophoretic profile in horses undergoing crotalid venom hyperimmunization.

Q3 Veterinary
Journal of Equine Science Pub Date : 2022-09-01 Epub Date: 2022-09-21 DOI:10.1294/jes.33.31
Monique Gonçalves Dos Santos Novelino Ramos, Sabrina Destri Emmerick Campos, Marcelo Abrahão Strauch, Leila Cardozo Ott, Daniel de Barros Macieira, Nayro Xavier de Alencar, Daniel Augusto Barroso Lessa
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引用次数: 1

Abstract

Indisputably, the use of antivenoms for the treatment of snakebite envenoming is beneficial for the victims. However, there are few studies addressing the effect of long-term hyperimmunization in inoculated horses. It is known that the injection of snake venoms and adjuvants leads to local and systemic reactions in horses, but little is known about the response of inflammatory proteins. The aim of this study was to evaluate serum proteins and the electrophoretic profile of horses undergoing crotalid venom hyperimmunization. Twenty horses were divided into two groups: an inoculated group, comprising ten horses that were already being used for production of a Crotalus sp. antivenom, and a control group, comprising ten animals that had never been used for hyperimmunization. All animals were clinically healthy and without laboratory abnormalities. Total protein and albumin concentrations were measured in serum. Serum globulins were obtained by calculation. Plasma fibrinogen estimates were determined by the heat precipitation method. Serum proteinograms were obtained using agarose gel electrophoresis. The results revealed a significant increase in the concentrations of total serum proteins, globulins, and β-globulins in the inoculated group, exceeding the reference values. There were slight increases in the α-1- and α-2-globulin subfractions in serum-producing horses, with no statistical significance. We also observed that horses used to produce hyperimmune plasma developed hypoalbuminemia, although the decrease in albumin production was not statistically significant. Our findings suggest that the continuous use of horses to produce crotalid antivenom may lead to a chronic inflammatory stimulus, with changes in plasma levels of inflammatory proteins.

马接受蛇毒过度免疫的血清蛋白和电泳谱。
毋庸置疑,使用抗蛇毒血清治疗蛇咬伤对受害者是有益的。然而,很少有研究解决长期过度免疫对接种马的影响。众所周知,注射蛇毒和佐剂会导致马的局部和全身反应,但对炎症蛋白的反应知之甚少。本研究的目的是评估接受蛇毒过度免疫的马的血清蛋白和电泳谱。20匹马被分为两组:一组接种了疫苗,包括10匹已经被用于生产Crotalus sp.抗蛇毒血清的马;另一组是对照组,包括10匹从未被用于过度免疫的动物。所有动物临床健康,无实验室异常。测定血清中总蛋白和白蛋白浓度。计算血清球蛋白。血浆纤维蛋白原用热沉淀法测定。琼脂糖凝胶电泳获得血清蛋白图。结果显示,接种组血清总蛋白、球蛋白和β-球蛋白浓度显著升高,超过参考值。产血清马α-1-和α-2球蛋白亚组分略有升高,但差异无统计学意义。我们还观察到,用于产生高免疫血浆的马出现了低白蛋白血症,尽管白蛋白产生的减少没有统计学意义。我们的研究结果表明,持续使用马生产crotalid抗蛇毒血清可能导致慢性炎症刺激,血浆炎症蛋白水平发生变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Equine Science
Journal of Equine Science Veterinary-Equine
CiteScore
1.60
自引率
0.00%
发文量
9
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