Enhanced detection of Taylorella equigenitalis by qPCR using 'Dry' swabs.

Q3 Veterinary
Ian Mawhinney, Anne Bollard
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引用次数: 0

Abstract

Detection of Taylorella equigenitalis (CEMO) in the horse uses genital swabs. These swabs traditionally have been put in Amies charcoal transport medium for detection by culture but are also used for PCR. We determined the suitability of swabs without transport medium (Dry swabs) for CEMO PCR compared to swabs in Amies charcoal transport medium. The experiment was a factorial design using swab type and dilution of organism in culture suspensions, done in two parts. Simulated genital swabs were prepared in the laboratory by dipping in pairs into culture suspensions containing T. equigenitalis with or without other organisms, and then inserting them into a sleeve either with or without transport medium. In study 1, the difference in Ct value for the two swab types was compared. In study 2 genital swab material was then also added to culture suspensions and the swab types again compared. The swabs were tested by a validated quantitative PCR method. The Ct value of the PCR test was used as the measure for comparison, and the effect of variables assessed with linear regression. There was an 7.7% (6.5-8.9) higher mean Ct value of TM versus Dry swabs (P<0.001) overall. The Ct difference was more marked at higher dilutions. Addition of genital swab material had no effect on the Ct value. Dry swabs perform at least as well for PCR as swabs in Amies charcoal transport medium, especially when relatively low numbers of organism are present, and are advantageous for routine sampling when culture is not being used.

Abstract Image

Abstract Image

用“干”拭子增强qPCR检测均匀泰勒菌。
使用生殖器拭子检测马的均匀泰勒菌(CEMO)。这些拭子传统上放在Amies木炭运输培养基中进行培养检测,但也用于PCR。我们确定了没有运输介质的拭子(干拭子)与在Amies木炭运输介质中的拭子相比对CEMO PCR的适用性。实验是一个因子设计,使用拭子类型和培养悬浮液中生物体的稀释,分两部分进行。模拟生殖器拭子是在实验室中制备的,将其成对地浸入含有或不含其他生物的相等螺旋体的培养悬液中,然后将其插入有或不含运输介质的套筒中。在研究1中,比较两种拭子类型Ct值的差异。在研究2中,将生殖器拭子材料也加入培养悬浮液中,并再次比较拭子类型。用经过验证的定量PCR方法对拭子进行检测。采用PCR检验的Ct值作为比较指标,采用线性回归评估变量的影响。与干拭子相比,TM的平均Ct值高出7.7% (6.5-8.9)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Equine Science
Journal of Equine Science Veterinary-Equine
CiteScore
1.60
自引率
0.00%
发文量
9
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