Fundamental Research最新文献

{"title":"Transcranial direct current stimulation and lesions hierarchically reorganize brain network dynamics with biological annotations","authors":"Jie Chen,&nbsp;Feixue Wang,&nbsp;Lei Zhao,&nbsp;Huijuan Zhang,&nbsp;Zhiyan Wang,&nbsp;Yilan Tang,&nbsp;Xiangyu Chang,&nbsp;Weiwei Ma,&nbsp;Yi Qiu,&nbsp;Yangyang Yi,&nbsp;Fanzhe Fu,&nbsp;Yipeng Yao,&nbsp;Fangyuan Cui,&nbsp;Yihuai Zou,&nbsp;Jin Cao,&nbsp;Yiheng Tu","doi":"10.1016/j.fmre.2025.02.017","DOIUrl":"10.1016/j.fmre.2025.02.017","url":null,"abstract":"<div><div>Mapping human brain function based on neuromodulation and lesions could reorganize brain network dynamics and uncover causal relationships with behaviors or symptoms. However, the principles of reorganization, neural mechanisms, and clinical implications underlying brain network dynamics in response to such modulations remain unclear. Here, we leveraged large-scale fMRI data and perturbation approaches to modulate brain network dynamics, investigating their associations with multi-level micro-architectural profiles including neurotransmitter receptors and transporters, cell types, laminar differentiation, microstructure, cortical expansion, and metabolism. We found that neuromodulation and lesions modulating the prefrontal and motor cortices led to distinct and opposing reorganizations in the topographic distribution of network dynamics along the sensorimotor-association hierarchy. Moreover, we observed a predominant co-localization between the reorganizations and micro-architectural properties, particularly neurotransmitter profiles. Behavioral analyses showed that the network dynamics of bilateral motor regions were associated with the motor functions of patients with lesions. These findings offer insights into the hierarchical reorganizational principle, cortical plasticity, and neurobiological basis underlying network dynamics, and potentially guide the development of alternative treatment approaches for complex brain disorders.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 3519-3528"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiotherapy followed by metformin neoadjuvant therapy drives superior pancreatic cancer ferroptosis and augments anti-tumor immunity","authors":"Haitao Zhu,&nbsp;Yi Gong,&nbsp;Xin Wang,&nbsp;Zining Zhang,&nbsp;Bo Wang,&nbsp;Wenjin Xu,&nbsp;Yuting Su,&nbsp;John E. Eriksson,&nbsp;Dongqing Wang,&nbsp;Shijie Wei,&nbsp;Yanfang Liu,&nbsp;Xiang Liao","doi":"10.1016/j.fmre.2025.12.010","DOIUrl":"10.1016/j.fmre.2025.12.010","url":null,"abstract":"<div><div>Although neoadjuvant therapy has achieved higher levels of resectability and improved the survival outcomes of pancreatic carcinoma patients, cancer cells' resistance to conventional cell death has limited its clinical efficiency. Thus, developing an optimal sequence of novel neoadjuvant therapy modalities to boost cancer cell death is urgently needed. The current work addresses this issue by developing a combination of radiotherapy with metformin as a novel neoadjuvant therapy and investigating its optimal sequencing treatment to boost cancer cell ferroptosis. Of the three treatment regimens investigated, the sequential treatment with RT followed by metformin (i.e. as opposed to metformin followed by RT, or simultaneous treatment with both RT and metformin) was most effective in eliciting ferroptosis in vitro and in animal models. Mechanically, RT followed by metformin is dependent on the inhibition of the AMPK-GOT1 axis, which further leads to the arrest of cancer cells in G1/S phase, and ultimately ferroptosis. Moreover, using a combination of patient samples and mouse models, RT followed by metformin enhanced the anti-tumor immune cells (IFNγ⁺CD8⁺T cells, DC cells, and M1 macrophage) infiltration and cytokines (IFN-γ and IL-2) level, inhibited the pro-tumor immune cells (PD-1⁺TIM3⁺ CD8⁺T cells and M2 macrophage) infiltration and cytokines (TGF-β and IL-10) level. Overall, the study developed an optimal sequencing of neoadjuvant therapy modality that induced superior cancer cell ferroptosis and boosted anti-tumor immunity, which presented a promising therapeutic approach for refractory tumors.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 3021-3033"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147902749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acoustic telemetry for marine animal and environmental monitoring: Technologies, challenges, and emerging trends","authors":"Abdelrahman Samy Ismail,&nbsp;Zhenting Ye,&nbsp;Amar N. Alsheavi,&nbsp;Yaxiaer Yalikun,&nbsp;Yang Yang,&nbsp;Chenjing Shang","doi":"10.1016/j.fmre.2026.05.019","DOIUrl":"10.1016/j.fmre.2026.05.019","url":null,"abstract":"<div><div>Acoustic telemetry is a fundamental tool for tracking marine animals, providing long-term data that support fisheries management, conservation, and ecosystem-based monitoring. Advances in acoustic tags, receiver arrays, and sensor-integrated transmitters have expanded the ability to quantify animal movement, habitat use, and physiological responses across diverse marine environments. However, the long-term deployment of telemetry systems remains constrained by limited power supply, device size, detection performance in complex habitats, and environmental interference. This review provides an in-depth analysis of the fundamental principles and system architectures of acoustic telemetry, with emphasis on tag design, sensing capabilities, positioning approaches, and data workflows. It further highlights major marine monitoring applications, including pollution impact assessment, climate change response, marine protected area (MPA) design, and combating illegal, unreported, and unregulated (IUU) fishing. To address persistent energy and durability limitations, the review synthesizes emerging advances in sustainable power strategies, including flexible, self-healing, stretchable, and biodegradable energy-harvesting and storage materials, as well as ocean-wave energy-harvesting technologies for autonomous, long-term deployment. By linking recent progress in acoustic telemetry with sustainable energy solutions, this paper identifies current challenges and future directions for resilient, environmentally responsible, next-generation marine observation platforms.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 3214-3240"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism-directed design and synthesis of a dinuclear copper porphyrin for efficient electrocatalytic hydrogen evolution reaction","authors":"Zhuangzhuang Chang,&nbsp;Xinyang Peng,&nbsp;Mengchun Zhang,&nbsp;Yuhan Xu,&nbsp;Haonan Qin,&nbsp;Qian-Cheng Luo,&nbsp;Xue-Peng Zhang,&nbsp;Wei Zhang,&nbsp;Yan-Zhen Zheng,&nbsp;Ulf-Peter Apfel,&nbsp;Rui Cao","doi":"10.1016/j.fmre.2025.08.002","DOIUrl":"10.1016/j.fmre.2025.08.002","url":null,"abstract":"<div><div>Developing efficient catalysts for the hydrogen evolution reaction (HER) is vital for a H₂-based energy system. Designing catalysts based on the HER mechanisms is required but has been rarely achieved. Herein, we report on a dinuclear Cu porphyrin for HER. We recently identified an intermolecular electron transfer mechanism in the HER with Cu porphyrins. We consider realizing intramolecular electron transfer between Cu porphyrins to improve HER. By linking two porphyrin units through an alkynyl group, we synthesized dinuclear Cu porphyrin <strong>1</strong> with two conjugated porphyrin units. Electrochemical studies confirm the effective interaction between the two Cu porphyrin units. Importantly, for HER electrocatalysis, the <em>i</em>_cat/<em>i</em>_p value with <strong>1</strong> (204) is seven times larger than that with its mononuclear analogue (29). Experimental and theoretical studies confirm intramolecular electron transfer in the HER with <strong>1</strong>. This work is therefore significant in presenting a mechanism-directed design of <strong>1</strong> as a highly active HER catalyst.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 2967-2973"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabricating polarization materials with subnanowires","authors":"Jiangxiao Li,&nbsp;Jiatao Zhang,&nbsp;Simin Zhang","doi":"10.1016/j.fmre.2025.03.021","DOIUrl":"10.1016/j.fmre.2025.03.021","url":null,"abstract":"<div><div>Polarization materials have widespread and crucial applications in sensing, display and optical devices. Subnanowires (SNWs) with polymer-analogue properties and unique self-assembly characteristics have become a potential candidate for fabricating freestanding and flexible polarization materials. Based on the intrinsic order, high anisotropy and inherent chirality, SNWs can be easily processed into well-organized and oriented assemblies through facile methods, which is the fabrication principle of SNW polarization materials. Through the Langmuir-Blodgett (LB) technique, wet-spinning method, directional coating method and evaporation-induced self-assembly, polarization materials of pure SNWs or SNWs incorporating with quantum dot (QD), quantum rod (QR), aggregation-induced emission luminogen (AIEgen) or liquid crystal (LC) can be successfully prepared. There are both critical challenges and opportunities for SNW-based polarization materials. Until now, developing new SNWs as building blocks to extend functions, exploiting more effective assembly approaches to enhance production efficiency and polarization performance, and further realizing precise regulation of the structures remain the main issues for developing SNW-based polarization materials. We hope this review will offer crucial insights into the fabrication of SNW-based polarization materials, shedding light on the development of high-performance polarization materials for advanced optical applications.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 2933-2941"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent research progress on perylene diimide-based photocatalytic materials","authors":"Huiyan Xu,&nbsp;Jian Zhang,&nbsp;Xiran Qiao,&nbsp;Longhua Ding,&nbsp;Aizhu Wang,&nbsp;Hong Liu,&nbsp;Xin Yu","doi":"10.1016/j.fmre.2025.06.005","DOIUrl":"10.1016/j.fmre.2025.06.005","url":null,"abstract":"<div><div>Perylene diimide (PDI) is an organic semiconductor with outstanding optical and electronic properties, including a rigid π-conjugated structure, high electron affinity, and chemical stability, which have garnered significant attention in the field of photocatalysis. This review provides a comprehensive overview of recent advancements in PDI-based photocatalytic materials, focusing on their structural characteristics and photocatalytic mechanisms. Despite their promise, PDI-based materials face challenges in practical applications, such as poor light absorption and high charge recombination rates. To address these issues, several strategies have been developed to enhance their photocatalytic performance, including the molecular design of various PDI polymer systems, the incorporation of co-catalysts to boost activity, the construction of heterostructures, synergistic Fenton reactions, and carbon-coupled PDI supramolecular photocatalysts. These approaches have led to significant improvements in the stability and efficiency of PDI-based photocatalysts. These approaches have led to significant improvements in the stability and efficiency of PDI-based photocatalysts. In practical applications, PDI-based materials show great potential in critical areas such as photocatalytic sterilization, environmental pollutant degradation, water splitting for hydrogen production, CO₂ reduction to renewable fuels, nitrogen fixation, and cancer treatment. These applications highlight the vast potential of PDI-based materials in both environmental remediation and renewable energy development. Finally, the review discusses future research directions, emphasizing the need for rational molecular design, optimization of heterostructure, and the development of novel composite materials as key strategies to further enhance performance. With continued research and development, PDI-based photocatalysts are poised to become a key technology in tackling global challenges such as energy shortages and environmental pollution.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 3271-3290"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryogen-free superconducting magnetic resonance imaging system: A review","authors":"Yaohui Wang,&nbsp;Pengfei Wu,&nbsp;Wenchen Wang,&nbsp;Hui Wang,&nbsp;Shunzhong Chen,&nbsp;Jinshui Sun,&nbsp;Feng Liu","doi":"10.1016/j.fmre.2025.05.006","DOIUrl":"10.1016/j.fmre.2025.05.006","url":null,"abstract":"<div><div>The development of cryogen-free superconducting magnetic resonance imaging (MRI) systems represents a significant milestone in MRI technology. By eliminating the need for liquid helium bath cooling, these magnet systems reduce the volume and weight of MRI machines. This advancement in superconducting magnet technology mitigates the safety risks associated with magnet quenching and lowers operational costs, offering substantial economic benefits. Cryogen-free MRI systems provide considerable convenience for clinical applications and scientific research, representing a mainstream trend in MRI technology advancement. Many research institutions worldwide are advancing various aspects of cryogen-free MRI projects, each tailored to specific application needs. This paper reviews recent progress in the research and development of cryogen-free MRI technology, including design schemes, technical features, and performance results. Furthermore, it presents a perspective on the challenges and prospects of cryogen-free MRI, offering guidance for future research and development in this promising field.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 3147-3155"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solar-driven butanedione-based disinfection: A by-product-free approach to pathogen control","authors":"Zewen Ji,&nbsp;Wenchang Zhang,&nbsp;Jianghua Yang,&nbsp;Peng Peng,&nbsp;Haoran Chen,&nbsp;Shujuan Zhang","doi":"10.1016/j.fmre.2025.03.003","DOIUrl":"10.1016/j.fmre.2025.03.003","url":null,"abstract":"<div><div>The imperative for environmentally friendly water disinfection methods has spurred the exploration of innovative solutions to mitigate microbial threats in water while simultaneously controlling the formation of harmful disinfection by-products (DBPs). This study introduces the combination of butanedione (BD) and solar irradiation as an effective method to inactivate pathogenic bacteria, including Gram-negative <em>Escherichia coli</em> and Gram-positive <em>Staphylococcus aureus</em>. Acetyl radicals produced by solar activation of BD homolytic cleavage combined with dissolved oxygen to form acetyl peroxyl radicals, which were the main reactive species in the disinfection process. These acetyl peroxyl radicals attack bacterial cell membranes, escalate intracellular reactive oxygen species, and induce oxidative degradation of vital intracellular components such as enzymes and nucleic acids. Remarkably, the BD/Solar system demonstrated exceptional disinfection efficacy across a range of water quality parameters, achieving rate constants that were 5.2–98.1 times greater than those of other advanced disinfection processes that utilize H₂O₂, peroxydisulfate, or periodate in combination with solar irradiation. Notably, there was no evidence of bacterial regrowth and no formation of typical halogenated DBPs after treatment. These findings highlight the potential of this novel solar-powered system as a green approach for controlling environmental pathogens in a variety of waters, especially in sunlit swimming pools.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 2950-2958"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Passive decoy state quantum secure direct communication","authors":"Jia-Wei Ying,&nbsp;Jin-Yu Wang,&nbsp;Yu-Xiang Xiao,&nbsp;Shi-Pu Gu,&nbsp;Xing-Fu Wang,&nbsp;Lan Zhou,&nbsp;Yu-Bo Sheng","doi":"10.1016/j.fmre.2025.12.008","DOIUrl":"10.1016/j.fmre.2025.12.008","url":null,"abstract":"<div><div>The decoy state method is an effective approach for addressing the source imperfections in quantum communication. By employing pulses of varying intensities to estimate error rates and yields, this method requires active modulation of the source intensity. However, the active modulation operation may introduce side-channel vulnerabilities, potentially allowing an eavesdropper to execute Trojan horse attacks. The passive decoy state method circumvents this issue by eliminating the need for active intensity modulation. In this paper, we propose a passive decoy state quantum secure direct communication (QSDC) protocol that generates weak coherent states with random intensities using two phase-randomized coherent pulses. Leveraging linear optics, our protocol facilitates straightforward implementation and is robust against eavesdropping side-channel attacks targeting source intensity modulators.We establish a system model for the protocol and derive its secrecy message capacity. Additionally, we optimize the input intensities of the protocol and obtain the maximum secrecy message capacity for each channel attenuation value. Compared with the previous protocol, our protocol exhibits an approximately 25% enhancement in the secrecy message transmission rate within a channel attenuation range of 6 dB. We also compare the performance of the three-decoy-state protocol with that of the infinite-decoy-state protocol. Within a channel attenuation range of 6.8 dB, the secrecy message capacity of the three-decoy-state protocol achieves approximately 94%–96% of that of the infinite-decoy-state.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 2878-2890"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of early-life tactile experience enrichment on autistic-like behaviors and synaptic alterations in Shank3 mutant mice","authors":"Mingyue Liu,&nbsp;Guaiguai Ma,&nbsp;Yangming Zhang,&nbsp;Kezi Li,&nbsp;Fei Li,&nbsp;Tao Peng,&nbsp;Congcong Qin,&nbsp;Violeta G López-Huerta,&nbsp;Mario A Arias-Garcia,&nbsp;Wenting Wang,&nbsp;Dayun Feng,&nbsp;Shengxi Wu,&nbsp;Baolin Guo","doi":"10.1016/j.fmre.2025.09.011","DOIUrl":"10.1016/j.fmre.2025.09.011","url":null,"abstract":"<div><div>Autism spectrum disorder (ASD) presents a significant therapeutic challenge due to limited treatment options. While non-pharmacological approaches based on sensory stimulation have shown promise in clinical practice, the underlying mechanisms remain poorly understood. This study explored the therapeutic potential of tactile experience enrichment (TEE) during specific developmental windows in <em>Shank3</em> KO mice, a model of ASD. We found that pre-weaning TEE robustly rescued core behavioral deficits, including social interaction and repetitive grooming, whereas juvenile TEE only improved social behavior. RNA sequencing of the anterior cingulate cortex (ACC) and dorsolateral striatum (DLS) revealed TEE-induced alterations in synaptic gene expression, including the upregulation of the Polo-like kinase 1 (PLK-1) pathway, which may modulate synaptic maturation. Furthermore, pre-weaning TEE restored dendritic spine density and morphology, along with synaptic transmission, in both the ACC and DLS. These findings indicate that early tactile enrichment, particularly during the pre-weaning period, can effectively alleviate core ASD-related behaviors in <em>Shank3</em> KO mice by modulating synaptic structure and function, highlighting its potential as a therapeutic intervention for ASD.</div></div>","PeriodicalId":34602,"journal":{"name":"Fundamental Research","volume":"6 5","pages":"Pages 3457-3470"},"PeriodicalIF":6.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}