Food Chemistry Molecular Sciences最新文献

{"title":"Quantitative transcriptomic and metabolomic analyses reveal the changes in Tibetan pork during refrigerated storage","authors":"Yuru Shui ,&nbsp;Yunxiao Xie ,&nbsp;Wanlin Cai ,&nbsp;Xuedong Gu ,&nbsp;Lei Liu ,&nbsp;Jinqiu Wang ,&nbsp;Fang Geng","doi":"10.1016/j.fochms.2025.100329","DOIUrl":"10.1016/j.fochms.2025.100329","url":null,"abstract":"<div><div>During refrigeration, postmortem quality changes of Tibetan pork are driven by coordinated transcriptional reprogramming that induces a metabolic shift from aerobic to anaerobic metabolism and activates the key signaling pathways regulating proteolysis and cell membrane integrity. To validate this hypothesis, pork samples were stored at 4 °C for 5 days and analyzed using multi-omics approaches. Metabolomic results revealed a 52% increase in total metabolite abundance after 5 days, accompanied by substantial accumulation of lysophospholipids, indicating severe membrane structural damage. Continuous protein degradation resulted in the release of free amino acids, which subsequently promoted the accumulation of two key biogenic amines, 1,5-diaminopentane (cadaverine) and tyramine. Transcriptomic analysis identified 5062 differentially expressed genes and revealed significant activation of several pathways, including PI3K-Akt and calcium signaling, in postmortem pork. These pathways collectively mediated extensive degradation of myofibrillar and extracellular matrix proteins. Overall, these findings systematically elucidate the molecular mechanisms underlying the development of tenderness and flavor in Tibetan pork, providing a theoretical basis for improving meat quality control under cold-chain conditions.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100329"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous proline regulates the antioxidation system and fruit quality of cherry tomatoes under chilling storage: Integrated physiological, metabolomic, and transcriptomic study","authors":"Suihua Huang ,&nbsp;Quanqing Deng ,&nbsp;Kaixin Bao ,&nbsp;Jianneng Chen ,&nbsp;Xuqiao Deng ,&nbsp;Xu Wang","doi":"10.1016/j.fochms.2025.100311","DOIUrl":"10.1016/j.fochms.2025.100311","url":null,"abstract":"<div><div>Cherry tomatoes fruits suffer from chilling stress during storage. Proline (Pro) is a vital amino acid to alleviate the stress. However, the biochemical mechanisms remained unclear. A field experiment was conducted with spraying distilled water (T0) or Pro solution during the growth period (T1); mature fruits were sampled at the date of harvest (D0) and stored at 4  °C for 20 d after harvest (D1). Results showed that the alanine content was increased by 1.7 %–8.0 % while the arginine content was reduced by 12.7 %–61.5 % under D1T1 compared with D1T0. The sugar acid ratio in D0T1 vs D1T1 was reduced to 65.9 % lower than that in D0T0 vs D1T0, although Pro treatment decreased the sugar acid ratio. Multivariate analysis revealed that PC1 accounted for 93.1 % in principal component analysis. The enzymatic activity of superoxide dismutase, ascorbate peroxidase, the amino acid content of glutamate and γ-aminobutyric acid were positively related to Pro content. Integrated metabolomic and transcriptomic analysis revealed that differentially expressed metabolites and genes were enriched in phenylalanine metabolism and lignin biosynthesis pathways. <em>CCoAOMT</em> was a putative gene responsible for the changes. Applications of Pro regulated the level of relevant amino acids and antioxidants, preserving the flavor quality after chilling storage in cherry tomatoes.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100311"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association studies for identification of QTLs and key candidate genes to improve grain quality in rice (Oryza sativa L.)","authors":"Supriya Sachdeva ,&nbsp;Rakesh Singh ,&nbsp;Harshita Singh ,&nbsp;Rakesh Bharadwaj ,&nbsp;Antil Jain ,&nbsp;Vikas K. Singh ,&nbsp;Uma Maheshwar Singh ,&nbsp;Arvind Kumar ,&nbsp;Gyanendra Pratap Singh","doi":"10.1016/j.fochms.2025.100313","DOIUrl":"10.1016/j.fochms.2025.100313","url":null,"abstract":"<div><div>Grain quality is the key concern for rice breeders and is paramount to consumer acceptability. We characterized a diverse subset of 198 rice accessions of 3 K Rice Genome Project (RGP) for grain quality attributes, specifically glycemic index %, total dietary fibre, oil %, protein, amylose, moisture %, phytate, phenol, and starch content. A set of 5,53,229 single nucleotide polymorphism (SNP) markers obtained from the 3 K RG 1 M filtered SNP dataset used for genome wide association studies (GWAS). Consequently, we discovered 200 Quantitative trait nucleotides (QTNs) associated with the traits mentioned above distributed across the genome. These QTNs were grouped into 26 Quantitative Trait Loci (QTL) clusters, of which 20 clusters validated with at least three GWAS methods were considered reliable. Furthermore, 869 putative candidate genes were identified, many of which overlapped between quality traits. Integrating the GWAS, RNA-seq and qRT-PCR results, we finally identified two important genes (<em>LOC_Os11g303700</em> and <em>LOC_Os11g30500</em>) associated with rice quality, and they may affect the grain quality by regulating the textural properties, appearance and eating quality. The findings of our study highlighted the role of molecular machinery in future rice breeding.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100313"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological, biochemical and transcriptomic analyses revealed key genes regulating ascorbic acid biosynthesis during sweet potato development","authors":"Qian Zhou ,&nbsp;Yajie Wang ,&nbsp;Letan Luo ,&nbsp;Yongxin Li ,&nbsp;Wanying Ge ,&nbsp;Huqing Yang","doi":"10.1016/j.fochms.2025.100310","DOIUrl":"10.1016/j.fochms.2025.100310","url":null,"abstract":"<div><div>Sweet potato (<em>Ipomoea batatas</em> Lam.) is a globally important food crop with higher ascorbic acid (AsA) content than most staple crops. However, the regulatory mechanisms of AsA accumulation in sweet potato remain unclear. In this study, we systematically examined the regulatory network of AsA metabolism by integrating physiological and biochemical analyses with transcriptome sequencing in four representative varieties. The results revealed significant variation in AsA accumulation during root development, ranging from 10.96 to 36.45 mg/100 g FW. Metabolic pathway analysis showed that AsA biosynthesis is mainly regulated through the L-galactose pathway, involving the genes <em>HK</em>, <em>PMI</em>, <em>PMM</em>, <em>GME</em>, and <em>GalLDH</em>, and the myo-inositol pathway represented by <em>MIOX</em>. Weighted gene co-expression network analysis (WGCNA) further identified <em>MIOX</em> as a highly connected hub gene within the AsA-associated network module. These findings provide important insights into the molecular basis of AsA metabolism in sweet potato and highlight promising candidate genes for future functional validation and molecular breeding efforts aimed at enhancing nutritional quality.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100310"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterising flavour differences in Mandarin fish muscle under live prey and formulated feed diets using flavouromics and metabolomics","authors":"Yang Cheng ,&nbsp;Junjian Dong ,&nbsp;Hetong Zhang ,&nbsp;Yuan Zhang ,&nbsp;Fengying Gao ,&nbsp;Fubao Wang ,&nbsp;Ziyang Wang ,&nbsp;Xing Ye ,&nbsp;Chengbin Wu ,&nbsp;Chengfei Sun","doi":"10.1016/j.fochms.2025.100287","DOIUrl":"10.1016/j.fochms.2025.100287","url":null,"abstract":"<div><div>Substituting formulated feed for trash fish in aquaculture has the potential to reduce farming costs and decrease dependence on natural resources. However, the impact of such feed substitution on muscle flavour remains unclear. This study is the first to utilise two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC × GC-TOF MS) and liquid chromatography–mass spectrometry (LC-MS) to analyse differences in muscle flavour between mandarin fish (<em>Siniperca chuatsi</em>) fed live prey and those fed formulated feed. The results identified 65 differential flavour compounds, with four key contributors: 2,3-butanedione, (E)-2-nonenal, furan, 2-pentyl-, and (E)-2-octenal. Metabolomic analysis revealed 139 differential metabolites, with significant upregulation of amino acid metabolites such as threonine and L-homoserine in the muscle of fish fed formulated feed. KEGG pathway enrichment highlighted glycine, serine and threonine metabolism, and aminoacyl-tRNA biosynthesis as critical pathways. Correlation analysis demonstrated significant associations between precursors like dihydroxyacetone phosphate and O-phosphoserine and key flavour compounds. The findings indicate that mandarin fish fed live prey exhibited a more pronounced nutty, buttery, ham-like aroma alongside a fresh, grassy scent, whereas those fed formulated feed showed a diminished earthy off-flavour. Dihydroxyacetone phosphate, L-homoserine, threonine, and linoleic acid were identified as important flavour precursors. Overall, these findings provide a theoretical basis for optimising the muscle flavour of mandarin fish in feed-based aquaculture.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100287"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and chemical analysis of olive oil produced by Greek olive cultivars: Linking genetic profiles with fatty acid composition and phenolic stability","authors":"Annia Tsolakou ,&nbsp;Kostas Ioannidis ,&nbsp;Sofia Lymperopoulou ,&nbsp;Panagiotis Diamantakos ,&nbsp;Georgios Kostelenos ,&nbsp;Eleni Melliou ,&nbsp;Prokopios Magiatis","doi":"10.1016/j.fochms.2025.100292","DOIUrl":"10.1016/j.fochms.2025.100292","url":null,"abstract":"<div><div>This pilot study explores the relationship between the genetic profiles of olive cultivars and monounsaturated fatty acid (MUFA) content of their oils, with emphasis on oxidative stability and phenolic integrity. Our working hypothesis was that cultivar-specific genetic variation in MUFA content, directly affects the oxidative stability of key phenolics, particularly oleocanthal and oleacein. To examine the association between genetic clustering and oleic acid content, eighty Greek olive cultivars cultivated under controlled nursery conditions were genotyped using eleven genomic simple sequence repeat (SSR) markers, and their fatty acid composition was determined by ¹H NMR. Phenolic stability was tested using oils with contrasting MUFA levels. Genetic analysis identified three clusters. Chemical cluster analysis, by dividing cultivars into three MUFA classes, revealed significant differences among divisions. Linking genetic and lipid profile groups highlighted notable overlap. This study reveals a clear experimental association between MUFA abundance in the olive matrix and its capacity to preserve phenolic integrity. By confirming the role of MUFA content in phenolic stability, our results provide a baseline reference for early-stage cultivar selection and for future breeding programs targeting enhanced olive oil quality.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100292"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zn2+ supplementation-mediated IZH4 enhances acid tolerance in Saccharomyces cerevisiae via membrane lipid remodeling: A multi-omics study","authors":"Tiantian Tian ,&nbsp;Chao Yang ,&nbsp;Jian-Lin Wu ,&nbsp;Shaoquan Liu","doi":"10.1016/j.fochms.2025.100324","DOIUrl":"10.1016/j.fochms.2025.100324","url":null,"abstract":"<div><div>Zinc is an essential micronutrient that serves as a vital cofactor in multiple metabolic pathways, including the mechanism mediated by <em>IZH</em> (Implicated in Zinc Homeostasis) genes and lipid biosynthesis. Previous studies revealed that <em>Saccharomyces cerevisiae</em> ET008-c54 (WT) exhibited excellent fermentation performance at low pH for acidic fruit wines, with <em>IZH4</em> identified as a key gene. This study investigated the specific role of <em>IZH4</em> mediated under Zn²⁺ supplementation in low-pH tolerance, using greengage plum wine as a model. Based on fermentation time and sensory evaluation, the optimal Zn²⁺ supplementation level was preliminarily determined to be 2 mg/L. Further metabolomics and flavoromics confirmed that Zn²⁺ supplementation at 2 mg/L was an effective strategy for enhancing the quality of greengage plum wine. On this basis, the effects of <em>IZH4</em> deletion on the growth behavior and membrane composition of the WT strain under Zn²⁺ supplementation were evaluated. The experimental findings revealed that the knockout strain WT-ΔIzh4 showed reduced growth at pH 2.5. Regarding the cellular lipid composition, WT-ΔIzh4 exhibited notably reduced levels of unsaturated fatty acids and ergosterol compared to WT, which resulted in less fluid membranes. Zn²⁺ supplementation increased the growth rate by 20.1 % and elevated ergosterol content by 15.2 % compared with WT-ΔIzh4, and further adjusted the ratio of unsaturated to saturated fatty acids to a level comparable to that of the WT strain. These results demonstrated the significant role of <em>IZH4</em> mediated under Zn²⁺ supplementation in the low pH tolerance of yeast by remodeling the cell membrane structure. This investigation offers fresh insights into the biological function of <em>IZH4</em> against low pH stress, and allows a better-defined target for subsequent genetic improvement of yeast.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100324"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145465458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the metabolic patterns of cashew apple ripening process: A comprehensive non-targeted metabolomics analysis","authors":"Haijie Huang ,&nbsp;Li Zhao ,&nbsp;Weijian Huang ,&nbsp;Xuejie Feng ,&nbsp;Fuchu Hu ,&nbsp;Ya Zhao ,&nbsp;Huiliang Li ,&nbsp;Yunlu Peng ,&nbsp;Yuhan Wang ,&nbsp;Zhongrun Zhang ,&nbsp;Yijun Liu","doi":"10.1016/j.fochms.2025.100296","DOIUrl":"10.1016/j.fochms.2025.100296","url":null,"abstract":"<div><div>Metabolite changes during the ripening process of cashew apples are crucial for their quality development. A total of 2379 metabolites were isolated and identified from fresh cashew apples at four different ripening stages using UHPLC-MS. Metabolite set enrichment analysis (MSEA) revealed that the differential metabolites in CA2_vs_CA1, CA3_vs_CA2, and CA4_vs_CA3 comparisons were mainly enriched in amino acids and peptides, steroids, pyrimidines, and fatty acids and conjugates, etc. Volcano plot analysis identified 631, 384, and 392 upregulated metabolites, and 625, 923, and 392 downregulated metabolites in CA2_vs_CA1, CA3_vs_CA2, and CA4_vs_CA3 comparisons, respectively. KEGG pathway enrichment analysis demonstrated that these differential metabolites were primarily involved in aminoacyl-tRNA biosynthesis, purine metabolism, and glycine, etc. Notably, the differential metabolites in CA4_vs_CA3 showed the highest enrichment in <span>d</span>-glutamine and D-glutamate metabolism, as well as phenylalanine. The metabolic profile of cashew apples revealed stage-specific patterns during ripening, offering key insights for optimizing harvest, storage, and processing.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100296"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic changes associated with health benefit properties in fresh and preserved mustard greens","authors":"Charanya Saekampang ,&nbsp;Vipawan Pimpak ,&nbsp;Poowadol Promwat ,&nbsp;Pongsakorn Kruaweangmol ,&nbsp;Narumon Phaonakrop ,&nbsp;Sittiruk Roytrakul ,&nbsp;Ulaiwan Withayagiat ,&nbsp;Paiboon Tunsagool","doi":"10.1016/j.fochms.2025.100295","DOIUrl":"10.1016/j.fochms.2025.100295","url":null,"abstract":"<div><h3>Background</h3><div>Mustard greens are gaining global popularity, but preservation methods like salting and pickling raise specific concerns related to high sodium content and microbial safety. This study conducted a proteomics analysis to identify health-beneficial proteins in mustard greens.</div></div><div><h3>Objective</h3><div>To explore protein profiles associated with health benefits in fresh and preserved mustard greens.</div></div><div><h3>Methods</h3><div>Shotgun proteomics combined with LC-MS/MS was applied to identify proteins, followed by statistical analysis using Welch's <em>t</em>-test with Benjamini–Hochberg false discovery rate correction. Unique proteins were visualized with a Venn diagram, annotated using the Gene Ontology (GO) database, and mapped to functional categories with Sankey diagrams. In silico validation of protein stability and gastrointestinal digestion was performed to assess the release of bioactive peptides.</div></div><div><h3>Results</h3><div>A total of 118 high-confidence proteins were identified, including 27 unique to fresh, 23 to salted, and 22 to pickled mustard greens. Unique proteins in fresh mustard greens were associated with carbohydrate, amino acid, and nucleotide metabolism. Pickled mustard greens had proteins linked to carbohydrate, lipid, glycerol, vitamin, and cofactor metabolism, while salted mustard greens had proteins involved in amino acid, nucleotide, lipid, and glycerol metabolism. In silico digestion revealed that approximately 19–20 % of peptide fragments contained bioactive motifs, predominantly angiotensin-converting enzyme (ACE) and dipeptidyl peptidase-IV (DPP-IV) inhibitory sequences.</div></div><div><h3>Conclusion</h3><div>Preserved mustard greens contain unique proteins associated with health benefits based on GO annotations, distinguishing their protein profiles from fresh mustard greens. This study provides insights into their functional properties, addressing concerns related to their consumption.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100295"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory role of calcium sulfide in ripening delay of postharvest bananas","authors":"Hong Xu ,&nbsp;Xueting Bi ,&nbsp;Junjie Xing ,&nbsp;Mengqian Guo ,&nbsp;Haoran Zhang ,&nbsp;Xuejie Zhang ,&nbsp;Wei Li ,&nbsp;Bingfu Lei","doi":"10.1016/j.fochms.2025.100297","DOIUrl":"10.1016/j.fochms.2025.100297","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S) has been demonstrated to delay ripening and senescence in various fruits, offering great capability for postharvest preservation. However, existing application methods face several limitations, such as unstable release, difficulty in dosage control, and safety concerns, and its regulatory mechanisms in fruit systems remain unclear. In this study, calcium sulfide (CaS) was used as a slow-release H₂S donor that gradually releases H₂S through reactions with airborne moisture and carbon dioxide to treat bananas. CaS treatment significantly downregulated key ethylene biosynthetic genes and corresponding enzymes (ACO and ACS), thereby reducing ethylene production. The expression of starch-degrading and cell wall-modifying genes was also suppressed, delaying starch breakdown and cell wall disassembly. Enzyme assays and transcriptomic analyses confirmed that CaS delays banana ripening through coordinated regulation at both transcriptional and biochemical levels. As a result, CaS treatment effectively extended shelf life and maintained fruit quality of bananas. These findings reveal the potential of CaS as a novel H₂S-releasing agent for postharvest preservation.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"11 ","pages":"Article 100297"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}