Food Chemistry Molecular Sciences最新文献

{"title":"Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link","authors":"Bárbara Verônica Cardoso de Souza ,&nbsp;Mariana de Morais Sousa ,&nbsp;José Augusto Gasparotto Sattler ,&nbsp;Ana Cristina Sousa Gramoza Vilarinho Santana ,&nbsp;Rusbene Bruno Fonseca de Carvalho ,&nbsp;José de Sousa Lima Neto ,&nbsp;Fernando de Matos Borges ,&nbsp;Iramaia Angelica Neri Numa ,&nbsp;Alessandra Braga Ribeiro ,&nbsp;Lívio César Cunha Nunes","doi":"10.1016/j.fochms.2022.100144","DOIUrl":"https://doi.org/10.1016/j.fochms.2022.100144","url":null,"abstract":"<div><p><em>Bauhinia forficata</em> Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of <em>B. forficata</em> Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MS<em>ⁿ</em>, evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (&lt;0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated <em>in vitro</em> digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of <em>B. forficata</em> is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666566222000727/pdfft?md5=ea7e040625b3cef91f0d5ae7eafc7dd9&pid=1-s2.0-S2666566222000727-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91590622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mistletoe infested Moringa oleifera and Terminalia catappa leaves supplemented diet enhances antioxidant and insulin-like peptide mRNA levels in Drosophila melanogaster","authors":"Olubukola H. Oyeniran ,&nbsp;Ganiyu Oboh ,&nbsp;Adedayo O. Ademiluyi ,&nbsp;Haruna I. Umar","doi":"10.1016/j.fochms.2022.100124","DOIUrl":"https://doi.org/10.1016/j.fochms.2022.100124","url":null,"abstract":"<div><p>Moringa and Almond are common plants of medicinal and economic value which are often infested with mistletoe. Host plants’ infestation could result in major differences in their phytoconstituents and biological activities. Thus, effects of mistletoe infestation on Moringa and Almond host plants supplemented diets on mRNA expression levels of <em>Drosophila</em> insulin-like peptide-2 (<em>Dilp2</em>), heat shock protein-70 (<em>Hsp70</em>) and superoxide dismutase (<em>Sod</em>) in diabetic-like flies were evaluated using quantitative real-time PCR system. Mistletoe infestation on host leaves caused significant upregulation of <em>Sod</em> and significant downregulation of <em>Hsp70</em> and <em>Dilp2</em> genes. Hence, we opined that infestation of Moringa and Almond trees with mistletoe resulted in improved expression level of antioxidant and insulin-like peptide genes. This may be the mechanism by which host plants caused enhanced regulation of circulating glucose and oxidative stress. Therefore, consumption of mistletoe infested Moringa and Almond host leaves could possibly offer better antioxidant and hypoglycemic effects.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666566222000521/pdfft?md5=e2f284a843859f2f3bace3207157d46f&pid=1-s2.0-S2666566222000521-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89988649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loop-mediated isothermal amplification (LAMP) for rapid and easy identification of Omphalotus japonicus","authors":"Yohei Sugano ,&nbsp;Kozue Sakata ,&nbsp;Kosuke Nakamura ,&nbsp;Aoi Hosokawa ,&nbsp;Hirokazu Kouguchi ,&nbsp;Tomohiro Suzuki ,&nbsp;Kazunari Kondo","doi":"10.1016/j.fochms.2022.100115","DOIUrl":"https://doi.org/10.1016/j.fochms.2022.100115","url":null,"abstract":"<div><p><em>Omphalotus japonicus</em> is a major toxic mushroom in Japan. When food poisoning caused by <em>O. japonicus</em> occurs, quick and accurate identification using a method that does not rely on morphological discrimination is required. Because the loop-mediated isothermal amplification (LAMP) method meets these requirements, we developed a LAMP method for detecting <em>O. japonicus</em>. Amplification occurred within 60 min, and the presence or absence of <em>O. japonicus</em> was confirmed within 2 h, including the DNA extraction protocol. The LAMP method did not show cross-reactivity with 13 species of edible mushrooms, had high specificity toward <em>O. japonicus</em>, and had sufficient detection sensitivity even in a mixed mushroom sample containing 1% <em>O. japonicus</em>. Additionally, <em>O. japonicus</em> could be detected in simulated food poisoning samples of heated and digested mushrooms, and in actual food poisoning residual samples.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666566222000430/pdfft?md5=21bb3a97132cfc22a2b562f603b963b7&pid=1-s2.0-S2666566222000430-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89988716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New evidence for dietary fatty acids in the neutrophil traffic between the bone marrow and the peripheral blood","authors":"Almudena Ortega-Gomez ,&nbsp;Sergio Lopez ,&nbsp;Lourdes M. Varela ,&nbsp;Sara Jaramillo ,&nbsp;Francisco J.G. Muriana ,&nbsp;Rocio Abia","doi":"10.1016/j.fochms.2022.100133","DOIUrl":"10.1016/j.fochms.2022.100133","url":null,"abstract":"<div><p>Chronic administration of a high-fat diet in mice has been established to influence the generation and trafficking of immune cells such as neutrophils in the bone marrow, the dysregulation of which may contribute to a wide range of diseases. However, no studies have tested the hypothesis that a short-term, high-fat diet could early modulate the neutrophil release from bone marrow at fasting and at postprandial in response to a high-fat meal challenge, and that the predominant type of fatty acids in dietary fats could play a role in both context conditions. Based on these premises, we aimed to establish the effects of different fats [butter, enriched in saturated fatty acids (SFAs), olive oil, enriched in monounsaturated fatty acids (MUFAs), and olive oil supplemented with eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids] on neutrophil navigation from bone marrow to blood in mice. The analysis of cellular models for mechanistic understanding and of postprandial blood samples from healthy volunteers for translational purposes was assessed. The results revealed a powerful effect of dietary SFAs in promotion the neutrophil traffic from bone marrow to blood via the CXCL2-CXCR2 axis. Dietary SFAs, but not MUFAs or EPA and DHA, were also associated with increased neutrophil apoptosis and bone marrow inflammation. Similar dietary fatty-acid-induced postprandial neutrophilia was observed in otherwise healthy humans. Therefore, dietary MUFAs might preserve bone marrow health and proper migration of bone marrow neutrophils early in the course of high-fat diets even after the intake of high-fat meals.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/61/main.PMC9467871.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40361471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the pathways influencing the berry color and firmness of grapevine cv. Flame Seedless treated with bioregulators using biochemical and RNA-Seq analysis under semi-arid subtropics","authors":"Vishal B. Mhetre ,&nbsp;V.B. Patel ,&nbsp;S.K. Singh ,&nbsp;Gyan P. Mishra ,&nbsp;M.K. Verma ,&nbsp;Chavlesh Kumar ,&nbsp;Anil Dahuja ,&nbsp;Sanjeev Kumar ,&nbsp;Rakesh Singh ,&nbsp;M. Wasim Siddiqui","doi":"10.1016/j.fochms.2022.100116","DOIUrl":"10.1016/j.fochms.2022.100116","url":null,"abstract":"<div><p>Plant bioregulators (PBRs) regulate developmental and physiological processes in plants. In this study, biochemical and transcriptomic analyses were conducted to evaluate the influence of PBRs [abscisic acid (ABA), benzothiadiazole (BTH), ethephon, and prohexadione-calcium (Pro-Ca)] on the grapevine cv. Flame Seedless under semi-arid subtropics. This study aims to see the effect of exogenous application of PBRs on overall berry quality, including uniformity of berry color. Uniform colored berries, the maximum total soluble solids (TSS) and total antioxidant activity (TAoA), and the highest total phenolics (TPC) and flavonoids (TFC) contents were obtained with the treatments, namely, 400 mg L^-1 ethephon and 400 mg L^-1 ABA. Further, RNA-Seq analysis has also identified some key DEGs like UFGT (<em>VIT_16s0039g02230</em>), GST (<em>VIT_04s0079g00690</em>), and chalcone synthase (CHS) (<em>VIT_05s0136g00260)</em> which were part of the anthocyanin biosynthesis pathway controlling grape berries color. Thus, ethephon (400 mg L^-1) and ABA (400 mg L^-1) were found promising for attaining greater uniformity in berry color development because of increased total anthocyanins content. In addition, they were also found associated with enhanced TAoA, TPC, and TFC. Hence, ethephon and ABA can be recommended for improving the berry quality.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/08/42/main.PMC9270244.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40607885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NMR and HPLC profiling of bee pollen products from different countries","authors":"Peng Lu ,&nbsp;Saki Takiguchi ,&nbsp;Yuka Honda ,&nbsp;Yi Lu ,&nbsp;Taichi Mitsui ,&nbsp;Shingo Kato ,&nbsp;Rina Kodera ,&nbsp;Kazuo Furihata ,&nbsp;Mimin Zhang ,&nbsp;Ken Okamoto ,&nbsp;Hideaki Itoh ,&nbsp;Michio Suzuki ,&nbsp;Hiroyuki Kono ,&nbsp;Koji Nagata","doi":"10.1016/j.fochms.2022.100119","DOIUrl":"10.1016/j.fochms.2022.100119","url":null,"abstract":"<div><p>Bee pollen, a beehive product collected from flowers by honeybees, contains over 250 biological substances, and has attracted increasing attention as a functional food. However, commercial bee pollen products are often multifloral, and samples from different countries vary significantly. There is no universal standard for objective quality assessment of bee pollen based on its chemical composition. Here, we report metabolomic analysis of 11 bee pollen samples from Spain, China, and Australia for quality control. The characteristics of the samples depend on the sucrose, nucleoside, amino acid, and flavanol concentrations. Bee pollen samples from Spain and Australia had higher sucrose and adenosine concentrations, whereas those from China had higher trigonelline, uridine, and cytidine concentrations. Interestingly, acetic acid was only detected in samples from China. These components can be used to identify the country of origin. The obtained profiles of the samples will contribute to universal standard development for bee pollen products.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40530329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2S albumin g13 polypeptide, less related to Fag e 2, can be eliminated in common buckwheat (Fagopyrum esculentum Moench) seeds","authors":"Fakhrul Islam Monshi ,&nbsp;Tomoyuki Katsube-Tanaka","doi":"10.1016/j.fochms.2022.100138","DOIUrl":"https://doi.org/10.1016/j.fochms.2022.100138","url":null,"abstract":"<div><p>2S albumin (g11, g13, g14, and g28) is an important allergen in common buckwheat (<em>Fagopyrum esculentum</em>). g13 is hydrophobic, rare in seeds, and may show distinct allergenicity from the others; therefore, we tried to eliminate this protein. Phylogenetic and property distance analyses indicated g13 is less related to g14 (Fag e 2) than g11/g28 is related to g14, particularly in the second domain containing the II and III α-helices. A null allele with a 531 bp insertion in the coding region was found for <em>g13</em> at an allele frequency of 2 % in natural populations of common buckwheat. The <em>g13_null</em> allele homozygote accumulated no g13 protein. A BLAST search for the 531 bp insertion suggested the insert-like sequence resided frequently in the buckwheat genome, including the self-incompatibility responsible gene <em>ELF3</em> in <em>Fagopyrum tataricum</em>. The <em>g13_null</em> insert-like sequence could, therefore, help in producing hypoallergenic cultivars, and expand the genetic diversity of buckwheat.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666566222000661/pdfft?md5=c82b1a190c5d757272005e4466af0fc5&pid=1-s2.0-S2666566222000661-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91590629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multipin cold plasma electric discharge on hydration properties of kodo millet flour: Modelling and optimization using response surface methodology and artificial neural network – Genetic algorithm","authors":"Samuel Jaddu,&nbsp;S. Abdullah,&nbsp;Madhuresh Dwivedi,&nbsp;Rama Chandra Pradhan","doi":"10.1016/j.fochms.2022.100132","DOIUrl":"10.1016/j.fochms.2022.100132","url":null,"abstract":"<div><p>The effect on functional properties of kodo millet flour was studied using multipin cold plasma electric reactor. The analysis was carried out at various levels of voltage (10–20 kV) and treatment time (10–30 min) for four different parameters such as water absorption capacity (WAC), oil absorption capacity (OAC), solubility index (SI) and swelling capacity (SC). Response surface methodology (RSM) and artificial neural network – genetic algorithm (ANN – GA) were adopted for modelling and optimization of process variables. The optimized values obtained from RSM were 20 kV and 17.9 min. On the contrary, 17.5 kV and 23.3 min were the optimized values obtained from ANN – GA. The RSM optimal values of WAC, OAC, SI and SC were 1.51 g/g, 1.40 g/g, 0.06 g/g and 3.68 g/g whereas optimized ANN – GA values were 1.51 g/g, 1.50 g/g, 0.06 g/g and 4.39 g/g, respectively. Infrared spectra, peak temperature, diffractograms and micrographs of both optimized values were analyzed and showed significant differences. ANN showed a higher value of R² and lesser values of other statistical parameters compared to RSM. Therefore, ANN – GA was treated as the best model for optimization and modelling of cold plasma treated kodo millet flour. Hence, the ANN – GA optimized values of cold plasma treated flour could be utilized for practical applications in food processing industries.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a6/14/main.PMC9465321.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40359205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two commercial methods for smooth-shelled mussels (Mytilus spp.) species identification","authors":"Cynthia M. Asorey ,&nbsp;Felipe Jilberto ,&nbsp;Ilka Haase ,&nbsp;Rainer Schubbert ,&nbsp;María Angélica Larraín ,&nbsp;Cristián Araneda","doi":"10.1016/j.fochms.2022.100121","DOIUrl":"10.1016/j.fochms.2022.100121","url":null,"abstract":"<div><p>Seafood international trade has increased the labeling requirements in standards and regulations to include product information that enable traders and consumers to make informed choices. The European Union (EU) Regulation No. 1379/2013 imposes the declaration of an official commercial designation and scientific names for all the fishery and aquaculture products to be offered for sale to the final consumers. DNA analyses are used to enforce this regulation and to test authenticity in processed foods. We compared the performance of two mono-locus approaches for species identification (SI) in 61 <em>Mytilus</em> mussels: the high-resolution melting analysis of the <em>polyphenolic adhesive protein</em> gene and the partial sequencing of the <em>histone H1C</em> gene. The <em>H1C</em> sequences were analyzed with five different methods. Both approaches show discrepancies in the identification of putative hybrids (0.0 &lt; κ &lt; 0.687 and 0.0 &lt; MCC &lt; 0.724). Excluding putative hybrids, methods show substantial to perfect agreement (0.772 &lt; κ &lt; 1.0 and 0.783 &lt; MCC &lt; 1.0). This study highlights the need to use standardized molecular tools, as well as to use multi-locus methods for SI of <em>Mytilus</em> mussels in testing laboratories.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40529377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cysteine residues are responsible for the sulfurous off-flavor formed in heated whey protein solutions","authors":"Chengkang Li ,&nbsp;Peter A. Paulsen ,&nbsp;Halise Gül Akıllıoğlu ,&nbsp;Søren B. Nielsen ,&nbsp;Kasper Engholm-Keller ,&nbsp;Marianne N. Lund","doi":"10.1016/j.fochms.2022.100120","DOIUrl":"10.1016/j.fochms.2022.100120","url":null,"abstract":"<div><p>Odor-active volatile sulfur compounds are formed in heated food protein systems. In the present study, hydrogen sulfide (H₂S) was found to be the most abundant sulfur volatile in whey protein solutions (whey protein isolate [WPI], a whey model system and single whey proteins) by gas chromatography-flame photometric detector (GC-FPD) analysis after heat treatments (60–90 °C for 10 min, 90 °C for 120 min and UHT-like treatment). H₂S was detected in WPI after heating at 90 °C for 10 min, and was significantly increased at higher heat load (90 °C for 120 min and the UHT-like treatment). Site-specific LC-MS/MS-based proteomic analysis was conducted, monitoring desulfurization reactions in these protein systems to investigate the mechanism of H₂S formation in heated WPI. Cysteine residues from beta-lactoglobulin were found to be responsible for the formation of H₂S in heated WPI, presumably via beta-elimination.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40529378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}