Food Chemistry Molecular Sciences最新文献

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Sprouts as probiotic carriers: A new trend to improve consumer nutrition 芽菜作为益生菌载体:改善消费者营养的新趋势
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-11-24 DOI: 10.1016/j.fochms.2023.100185
Stephany Nefertari Chávez García , Raúl Rodríguez-Herrera , Sendar Nery Flores , Sonia Yesenia Silva-Belmares , Sandra Cecilia Esparza-González , Juan A. Ascacio-Valdés , Adriana C. Flores-Gallegos
{"title":"Sprouts as probiotic carriers: A new trend to improve consumer nutrition","authors":"Stephany Nefertari Chávez García ,&nbsp;Raúl Rodríguez-Herrera ,&nbsp;Sendar Nery Flores ,&nbsp;Sonia Yesenia Silva-Belmares ,&nbsp;Sandra Cecilia Esparza-González ,&nbsp;Juan A. Ascacio-Valdés ,&nbsp;Adriana C. Flores-Gallegos","doi":"10.1016/j.fochms.2023.100185","DOIUrl":"https://doi.org/10.1016/j.fochms.2023.100185","url":null,"abstract":"<div><p>Over the past few decades, efforts to eradicate hunger in the world have led to the generation of sustainable development goals to reduce poverty and inequality. It is estimated that the current coronavirus pandemic could add between 83 and 132 million to the total number of undernourished people in the world by 2021. Food insecurity is a contributing factor to the increase in malnutrition, overweight and obesity due to the quality of diets to which people have access. It is therefore necessary to develop functional foods that meet the needs of the population, such as the incorporation of sprouts in their formulation to enhance nutritional quality. Germination of grains and seeds can be used as a low-cost bioprocessing technique that provides higher nutritional value and better bioavailability of nutrients. Consequently, the manuscript describes relevant information about the germination process in different seeds, the changes caused in their nutritional value and the use of techniques within the imbibition phase to modify the metabolic profiles within the sprouts such as inoculation with lactic acid bacteria and yeasts, to generate a functional symbiotic food.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"7 ","pages":"Article 100185"},"PeriodicalIF":3.3,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666566223000254/pdfft?md5=11efc6027d05e0715b2f07c868fda242&pid=1-s2.0-S2666566223000254-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138467036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Special Issue: Polyphenols in the development of functional foods and impact on health and nutrition in South and Central Americas’ 特刊:功能性食品开发中的多酚及其对南美洲和中美洲健康和营养的影响
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-10-03 DOI: 10.1016/j.fochms.2023.100184
Cinthia Baú Betim Cazarin, Lilian Regina Barros Mariutti
{"title":"Special Issue: Polyphenols in the development of functional foods and impact on health and nutrition in South and Central Americas’","authors":"Cinthia Baú Betim Cazarin,&nbsp;Lilian Regina Barros Mariutti","doi":"10.1016/j.fochms.2023.100184","DOIUrl":"10.1016/j.fochms.2023.100184","url":null,"abstract":"","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"7 ","pages":"Article 100184"},"PeriodicalIF":3.3,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/49/ac/main.PMC10563040.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41214765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid detection methods for foodborne pathogens based on nucleic acid amplification: Recent advances, remaining challenges, and possible opportunities 基于核酸扩增的食源性病原体快速检测方法:最新进展、剩余挑战和可能的机遇
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-09-18 DOI: 10.1016/j.fochms.2023.100183
Nodali Ndraha , Hung-Yun Lin , Chen-Yow Wang , Hsin-I Hsiao , Han-Jia Lin
{"title":"Rapid detection methods for foodborne pathogens based on nucleic acid amplification: Recent advances, remaining challenges, and possible opportunities","authors":"Nodali Ndraha ,&nbsp;Hung-Yun Lin ,&nbsp;Chen-Yow Wang ,&nbsp;Hsin-I Hsiao ,&nbsp;Han-Jia Lin","doi":"10.1016/j.fochms.2023.100183","DOIUrl":"10.1016/j.fochms.2023.100183","url":null,"abstract":"<div><p>This article presents a review of recent advancements in the utilization of NAA-based techniques for detecting foodborne pathogens in food products, focusing on studies conducted within the past five years. This review revealed that recent research efforts have primarily aimed at enhancing sensitivity and specificity by improving sample pre-treatment/preparation, DNA isolation, and readout methods. Isothermal-based amplification methods, such as LAMP, RPA, RAA, and RCA, have emerged as promising approaches, providing rapid results within one h and often demonstrating comparable or superior sensitivity to conventional or qPCR methods. However, the attention paid to specific pathogens varies, with <em>Salmonella</em> spp., <em>Listeria</em> spp., <em>E. coli</em>, and <em>V. parahaemolyticus</em> receiving more focus than norovirus and other similar pathogens. NAA-based methods have the potential to significantly contribute to food safety and public health protection. However, further advancements are necessary to fully realize their benefits.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"7 ","pages":"Article 100183"},"PeriodicalIF":3.3,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41138600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Reproducibility of next-generation-sequencing-based analysis of a CRISPR/Cas9 genome edited oil seed rape 基于CRISPR/Cas9基因组编辑的油菜籽下一代测序分析的可重复性
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-09-11 DOI: 10.1016/j.fochms.2023.100182
Steffen Pallarz , Stefan Fiedler , Daniela Wahler , Jörn Lämke , Lutz Grohmann
{"title":"Reproducibility of next-generation-sequencing-based analysis of a CRISPR/Cas9 genome edited oil seed rape","authors":"Steffen Pallarz ,&nbsp;Stefan Fiedler ,&nbsp;Daniela Wahler ,&nbsp;Jörn Lämke ,&nbsp;Lutz Grohmann","doi":"10.1016/j.fochms.2023.100182","DOIUrl":"10.1016/j.fochms.2023.100182","url":null,"abstract":"<div><p>Next-generation-sequencing (NGS) becomes increasingly important for laboratories tasked with the detection of genetically modified organisms (GMOs) in food, feed and seeds. Its implementation into standardized workflows demands reliable intra- and inter-laboratory reproducibility. Here, we analyze the reproducibility of short- and long-read targeted NGS and long-read whole genome sequencing (WGS) data between three independent laboratories. Replicate samples were submitted for sequencing and comparatively analyzed. The targeted-NGS-samples consisted of oil seed rape (OSR) sampled from a commodity shipment spiked with a genome edited (GE) OSR and the WGS-samples consisted of leaf material from the GMOs’ parental line. All laboratories delivered highly reproducible high-quality targeted NGS data with little variation. The detection of GMO-related sequences works well regardless of the facility, while the mapping to the complex genome is superior using long read data. Long read WGS is currently not suitable for routine use in enforcement laboratories, due to a large inter-laboratory variation.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"7 ","pages":"Article 100182"},"PeriodicalIF":3.3,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/bd/main.PMC10562171.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41214764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device 在微阵列芯片PCR导向的微流控横向流动条带装置上精确分子识别不同的肉类掺假而不携带污染物
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-08-12 DOI: 10.1016/j.fochms.2023.100180
Hanling Wang , Xianzhuo Meng , Li Yao , Qian Wu , Bangben Yao , Zhaoran Chen , Jianguo Xu , Wei Chen
{"title":"Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device","authors":"Hanling Wang ,&nbsp;Xianzhuo Meng ,&nbsp;Li Yao ,&nbsp;Qian Wu ,&nbsp;Bangben Yao ,&nbsp;Zhaoran Chen ,&nbsp;Jianguo Xu ,&nbsp;Wei Chen","doi":"10.1016/j.fochms.2023.100180","DOIUrl":"10.1016/j.fochms.2023.100180","url":null,"abstract":"<div><p>Meat adulteration-based food fraud has recently become one of the global major economical, illegal, religious, and public health concerns. In this work, we developed a microarray chip polymerase chain reaction (PCR)-directed microfluidic lateral flow strip (LFS) device that facilitates the accurate and simultaneous identification of beef adulterated with chicken, duck, and pork, especially in processed beef products. To realize this goal, four pairs of amplification primers were designed and applied for specifically amplifying genomic DNA extracted from mixed meat powders in microarray chip. With the prominent advantage of this device lies in the flexible combination and integration of sample loading, detection, and reporting in microstructures, all the DNA amplicons can be individually visualized on the LFS unit, leading to the appearance of test lines (T<sup>C</sup> line, T<sup>D</sup> line, T<sup>P</sup> line, or T<sup>B</sup> line) as well as the control line (C line) for the species identification and quantification in beef products. Based on this new method, the adulterants were successfully distinguished and identified in mixtures down to 0.01% (wt.%) while the carryover aerogel contamination in routine molecular diagnostic laboratories was effectively avoided. The practicability, accuracy, and reliability of the device were further confirmed by using real-time PCR as a gold standard control on the successful identification of 50 processed ground meat samples sourced from local markets. The method and device proposed herein could be a useful tool for on-site identification of food authentication.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"7 ","pages":"Article 100180"},"PeriodicalIF":3.3,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10471925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10506592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Validating duplex-PCR targeting ND2 for bovine and porcine detection in meat products 验证针对ND2的双链PCR在肉制品中检测牛和猪
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-08-12 DOI: 10.1016/j.fochms.2023.100181
Farouq Heidar Barido , Desti Desti , Ahmad Pramono , Zakaria Husein Abdurrahman , Slamet Diah Volkandari , Muhammad Cahyadi
{"title":"Validating duplex-PCR targeting ND2 for bovine and porcine detection in meat products","authors":"Farouq Heidar Barido ,&nbsp;Desti Desti ,&nbsp;Ahmad Pramono ,&nbsp;Zakaria Husein Abdurrahman ,&nbsp;Slamet Diah Volkandari ,&nbsp;Muhammad Cahyadi","doi":"10.1016/j.fochms.2023.100181","DOIUrl":"https://doi.org/10.1016/j.fochms.2023.100181","url":null,"abstract":"<div><p>Food authentication is a mandatory effort to assure the fair-trade. This study developed a duplex polymerase chain reaction (PCR) from the NADH dehydrogenase subunit 2 (<em>ND2</em>) gene to amplify specific segments of a cattle and porcine DNA. A universal forward primer composed of nineteen base pairs (bp) (3′-CCAAACACAACTCCGAAAA-5′) and species-specific reverse primers composed of twenty (3′-CCAAACACAACTCCGAAAA-5′) and twenty-one (3′-TGGCAAGAATTAGGACGGTTA-5′) bp were used to limit the amplified DNA segment for porcine and cattle. The PCR reaction would generate a product with a profile of 168 and 227 bp, respectively. To investigate the accuracy and limit of detection, an <em>in vitro</em> experiment was conducted using simplex and duplex PCR on commercial meatballs randomly purchased from a commercial market in Surakarta, Indonesia. The findings of this study indicated that <em>ND2</em> could be used as an alternative genetic marker for the identification of porcine and beef species in meat-derived products.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"7 ","pages":"Article 100181"},"PeriodicalIF":3.3,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50170568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preharvest 24-epibrassinolide treatment prolongs harvest duration and shelf life in sweet corn 收获前24表油菜素内酯处理延长甜玉米收获期和保质期
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-08-07 DOI: 10.1016/j.fochms.2023.100179
Ruiqiu Fang , Guihua Lv , Xianwen Zhang , Jianjian Chen , Xiaolong Chen , Bin Wang
{"title":"Preharvest 24-epibrassinolide treatment prolongs harvest duration and shelf life in sweet corn","authors":"Ruiqiu Fang ,&nbsp;Guihua Lv ,&nbsp;Xianwen Zhang ,&nbsp;Jianjian Chen ,&nbsp;Xiaolong Chen ,&nbsp;Bin Wang","doi":"10.1016/j.fochms.2023.100179","DOIUrl":"10.1016/j.fochms.2023.100179","url":null,"abstract":"<div><p>Sweet corn is perishable and have limited harvest duration and shelf life due to their quality deterioration. Reactive oxygen species (ROS) are one of the most predominant factors for maintaining quality of sweet corn during and after harvest. Brassinosteroids (BRs) can enhance the activity of antioxidant enzymes and decrease the ROS level in plants. In this study, we found that a bioactive BR (24-epibrassinolide, EBR) treatment before harvest markedly inhibited change of quality indicators (MDA content, weight loss rate, and soluble sugar content) during and after harvest. Further analysis revealed that EBR promoted the activity and transcriptions of antioxidant enzymes, maintaining lower ROS level in kernels. Meanwhile, exogenous EBR increased the expression level of genes controlling sucrose transport in sweet corn kernels. Bioinformatics and binding analysis identified that BR transcription factor ZmBES1/ZmBZR1-10 might potentially bind to and upregulate transcriptions of antioxidant enzyme genes including <em>SOD</em> and <em>POD</em> genes, and sucrose transport-related genes including <em>SUT</em> and <em>SWEET</em> genes. These results indicated that exogenous application of EBR ameliorates quality during and after harvest by improving the antioxidant capacity and photosynthetic assimilates accumulation rate of sweet corn, thus prolonging harvest duration and shelf life in sweet corn.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"7 ","pages":"Article 100179"},"PeriodicalIF":3.3,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cb/48/main.PMC10423688.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10003967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4–MyD88 expression in macrophages 白鳕水溶性蛋白通过降低巨噬细胞中TLR4-MyD88的表达来抑制脂多糖诱导的炎症
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-07-30 DOI: 10.1016/j.fochms.2023.100165
Masataka Ono , Satomi Watari , Mizuho Nishizawa-Higashi , Tatsuya Konishi , Yoshinori Takahashi , Hiroki Saeki , Ga-Hyun Joe
{"title":"Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4–MyD88 expression in macrophages","authors":"Masataka Ono ,&nbsp;Satomi Watari ,&nbsp;Mizuho Nishizawa-Higashi ,&nbsp;Tatsuya Konishi ,&nbsp;Yoshinori Takahashi ,&nbsp;Hiroki Saeki ,&nbsp;Ga-Hyun Joe","doi":"10.1016/j.fochms.2023.100165","DOIUrl":"https://doi.org/10.1016/j.fochms.2023.100165","url":null,"abstract":"<div><p>Water-soluble protein (WSP) from fish meat is abundant in the waste effluent generated via the surimi manufacturing process. This study investigated the anti-inflammatory effects and mechanisms of fish WSP using primary macrophages (MΦ) and animal ingestion. MΦ were treated with digested-WSP (d-WSP, 500 µg/mL) with or without lipopolysaccharide (LPS) stimulation. For the ingestion study, male ICR mice (5 weeks old) were fed 4% WSP for 14 days following LPS administration (4 mg/kg body weight). d-WSP decreased the expression of <em>Tlr4</em>, an LPS receptor. Additionally, d-WSP significantly suppressed the secretion of inflammatory cytokines, phagocytic ability, and <em>Myd88</em> and <em>Il1b</em> expressions of LPS-stimulated macrophages. Furthermore, the ingestion of 4% WSP attenuated not only LPS-induced IL-1β secretion in the blood but also <em>Myd88</em> and <em>Il1b</em> expressions in the liver. Thus, fish WSP decreases the expressions of the genes involved in the TLR4–MyD88 pathway in MΦ and the liver, thereby suppressing inflammation.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"6 ","pages":"Article 100165"},"PeriodicalIF":3.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49856158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of metabolic and transcription levels provides insights into the interactions of plant hormones and crosstalk with MAPKs in the early signaling response of cherry tomato fruit induced by the yeast cell wall 分析酵母细胞壁诱导的樱桃番茄果实早期信号反应中植物激素和串扰与MAPKs的相互作用,提供了代谢和转录水平的见解
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-07-30 DOI: 10.1016/j.fochms.2022.100160
Keyu Sun , Xue Zhang , Ze Wei , Ziwuzhen Wang , Jifeng Liu , Jian Liu , Jianhua Gao , Jun Guo , Xin Zhao
{"title":"Analysis of metabolic and transcription levels provides insights into the interactions of plant hormones and crosstalk with MAPKs in the early signaling response of cherry tomato fruit induced by the yeast cell wall","authors":"Keyu Sun ,&nbsp;Xue Zhang ,&nbsp;Ze Wei ,&nbsp;Ziwuzhen Wang ,&nbsp;Jifeng Liu ,&nbsp;Jian Liu ,&nbsp;Jianhua Gao ,&nbsp;Jun Guo ,&nbsp;Xin Zhao","doi":"10.1016/j.fochms.2022.100160","DOIUrl":"10.1016/j.fochms.2022.100160","url":null,"abstract":"<div><p>Yeast cell walls (YCW) are promising bio-based elicitors for controlling post-harvest fruit decay. In this study, 1% YCW induction increased the resistance of cherry tomato fruits, reducing disease incidence by 66%. This study aimed to explore the interaction of hormones and crosstalk with MAPKs (mitogen-activated protein kinases) in the early response of resistance regulation in cherry tomato fruits treated with YCW and U0126. We analyzed the temporal changes in hormone content, the expression of critical genes involved in phytohormone biosynthesis, and signal transduction in cherry tomato fruits response to the induction. Results revealed that jasmonic acid (JA) and brassinosteroids (BR) significantly regulated early resistance response in fruit induced by 1% YCW. The salicylic acid (SA) pathway is inhibited by the activation of the JA pathway. JA and SA signaling pathway crosstalk with the MAPK3 pathway. BR plays an essential role in the regulation of fruit resistance. The BR pathway may function independently when JA/SA and MAPK3 pathways are inhibited.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"6 ","pages":"Article 100160"},"PeriodicalIF":3.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/07/9a/main.PMC9816665.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10564911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Insertion of ten amino acids into 13S globulin zero-repeat subunit improves trypsin digestibility in common buckwheat (Fagopyrum esculentum Moench) seeds 在13S球蛋白零重复亚基中插入10个氨基酸可提高普通荞麦种子胰蛋白酶的消化率
IF 3.3
Food Chemistry Molecular Sciences Pub Date : 2023-07-30 DOI: 10.1016/j.fochms.2022.100159
Takeyuki Okada , Fakhrul Islam Monshi , Syuto Kudo , Tomoyuki Katsube-Tanaka
{"title":"Insertion of ten amino acids into 13S globulin zero-repeat subunit improves trypsin digestibility in common buckwheat (Fagopyrum esculentum Moench) seeds","authors":"Takeyuki Okada ,&nbsp;Fakhrul Islam Monshi ,&nbsp;Syuto Kudo ,&nbsp;Tomoyuki Katsube-Tanaka","doi":"10.1016/j.fochms.2022.100159","DOIUrl":"https://doi.org/10.1016/j.fochms.2022.100159","url":null,"abstract":"<div><p>The 13S globulin zero-repeat subunit is resistant to trypsin and may have higher allergenicity than the 1–6 tandem repeat subunits in common buckwheat (<em>Fagopyrum esculentum</em> Moench). To explore alleles useful for lowering allergenicity, amplicon deep sequencing targeting the zero-repeat subunit gene was conducted in bulked genomic DNA from eight cultivars and landraces. The analysis identified a unique allele encoding a zero-repeat subunit with 10 amino acid insertion (10aa) at a position equivalent to the tandem repeat insertion. Prediction of its 3-D structure suggested that 10aa changes the β-hairpin structure in the non-10aa (native) subunit to a random coil, which is also found in 1- and 3- repeat subunits. Homozygotes of the 10aa allele were developed and showed that the 10aa subunit was more digestible than the native subunit. However, the 10aa subunit was still less digestible than the 1–6 repeat subunits, suggesting needs to explore unfunctional alleles.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"6 ","pages":"Article 100159"},"PeriodicalIF":3.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49856152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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