Molecular Horticulture最新文献

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Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes. 多组学分析揭示了控制四种不同西瓜果肉颜色表型的双重遗传位点。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-14 DOI: 10.1186/s43897-025-00166-y
Na Li, Shilai Xing, Gaofei Sun, Jianli Shang, Jia-Long Yao, Nannan Li, Dan Zhou, Yu Wang, Yuan Lu, Jinpeng Bi, Jiming Wang, Hongfeng Lu, Shuangwu Ma
{"title":"Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes.","authors":"Na Li, Shilai Xing, Gaofei Sun, Jianli Shang, Jia-Long Yao, Nannan Li, Dan Zhou, Yu Wang, Yuan Lu, Jinpeng Bi, Jiming Wang, Hongfeng Lu, Shuangwu Ma","doi":"10.1186/s43897-025-00166-y","DOIUrl":"10.1186/s43897-025-00166-y","url":null,"abstract":"<p><p>Watermelon fruit flesh displays various colors. Although genetic loci underlying these variations are identified, the molecular mechanism remains elusive. Here, we assembled a chromosome-scale reference genome of an elite watermelon and developed integrated genetic maps using single nucleotide polymorphism (SNP) and structural variation markers. Several key genetic varients for fruit shape and flesh color were identified. Two variants associated with flesh color were further studied, including one copy number variant (CNV, a triplicate of 1.2 kb DNA) in the promoter region of REDUCED CHLOROPLAST COVERAGE 2 (ClREC2) and one SNP in Lycopene β-Cyclase (ClLCYB) coding region. These two variants together explained 99.7% of the flesh color variations in 314 watermelon accessions. The SNP in ClLCYB was the same as previously reported, disrupting ClLCYB function. The CNV could strongly enhance ClREC2 expression, consequently increasing the expression of carotenoid biosynthesis genes, the number of plastoglobules within chromoplasts, and carotenoid level in mature fruit flesh. Finally, we proposed a \"two-switch\" genetic model by integrating two major causative loci, which can explain the formation of the four main flesh colors in different watermelon accessions. These results provide new insights into the regulation of carotenoid biosynthesis and color formation in plants.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"46"},"PeriodicalIF":10.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new climate for genomic and epigenomic innovation in grapevine. 葡萄基因组学和表观基因组学创新的新气象。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-12 DOI: 10.1186/s43897-025-00171-1
Maximilian Schmidt, Timo Strack, Haylie Andrews, Lee T Hickey, Peter A Crisp, Kai P Voss-Fels
{"title":"A new climate for genomic and epigenomic innovation in grapevine.","authors":"Maximilian Schmidt, Timo Strack, Haylie Andrews, Lee T Hickey, Peter A Crisp, Kai P Voss-Fels","doi":"10.1186/s43897-025-00171-1","DOIUrl":"https://doi.org/10.1186/s43897-025-00171-1","url":null,"abstract":"","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"44"},"PeriodicalIF":10.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more. 橙家族蛋白:多功能伴侣塑造植物类胡萝卜素水平,质体发育,抗逆性等。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-09 DOI: 10.1186/s43897-025-00169-9
Emalee Wrightstone, Lilin Xu, Sombir Rao, Abhijit Hazra, Li Li
{"title":"ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more.","authors":"Emalee Wrightstone, Lilin Xu, Sombir Rao, Abhijit Hazra, Li Li","doi":"10.1186/s43897-025-00169-9","DOIUrl":"https://doi.org/10.1186/s43897-025-00169-9","url":null,"abstract":"<p><p>ORANGE (OR) family proteins are DnaJE1 molecular chaperones ubiquitous and highly conserved in all plant species, indicating their important roles in plant growth and development. OR proteins have been found to exert multiple functions in regulating carotenoid and chlorophyll biosynthesis, plastid development, and stress tolerance, with additional functions expected to be discovered. As molecular chaperones, OR proteins directly influence the stability of their target proteins via their holdase activity and may perform other molecular roles through unknown mechanisms. Exploration of OR has uncovered novel mechanisms underlying core plant metabolism pathways and expanded our understanding of processes linked to plastid development. Continued investigation of OR family proteins will not only reveal new functions of molecular chaperones but also provide pioneering tools for crop improvement. Thus, OR family proteins offer a distinctive opportunity to comprehend molecular chaperones in modulating various metabolic and developmental processes and exemplify the importance of chaperones in crop development and adaptability. This review briefly details the history of OR family proteins, highlights recent advancements in understanding their myriad of functions, and discusses the prospects of this fascinating group of chaperones towards generating innovative, more nutritious, and resilient crops alongside other agronomically important traits.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"43"},"PeriodicalIF":10.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
C2H2-type zinc finger protein transcription factor MdZAT1 plays a negative role in anthocyanin biosynthesis in apple. c2h2型锌指蛋白转录因子MdZAT1在苹果花青素生物合成中起负向作用。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-08 DOI: 10.1186/s43897-025-00150-6
Yanxue Ren, Wenping Huo, Zhongkang Wang, Shasha Liu, Yizhou Chen, Xiaolong Xu, Hongmin Hou, Chaohua Dong, Jihua Xu, Min Chen, Yugang Zhang, Shenghui Jiang
{"title":"C2H2-type zinc finger protein transcription factor MdZAT1 plays a negative role in anthocyanin biosynthesis in apple.","authors":"Yanxue Ren, Wenping Huo, Zhongkang Wang, Shasha Liu, Yizhou Chen, Xiaolong Xu, Hongmin Hou, Chaohua Dong, Jihua Xu, Min Chen, Yugang Zhang, Shenghui Jiang","doi":"10.1186/s43897-025-00150-6","DOIUrl":"https://doi.org/10.1186/s43897-025-00150-6","url":null,"abstract":"","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"28"},"PeriodicalIF":10.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryptochrome-mediated blue light regulates cell lignification via PbbHLH195 activation of the PbNSC in pear fruits. 隐色素介导的蓝光通过PbbHLH195激活梨果实PbNSC调控细胞木质化。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-07 DOI: 10.1186/s43897-025-00149-z
Qi Wang, Xinyi Wu, Mei Ren, Fanghang Zhang, Yang Zhang, Yueyang Wang, Wen Li, Zhihua Xie, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Yingying Niu, Shutian Tao
{"title":"Cryptochrome-mediated blue light regulates cell lignification via PbbHLH195 activation of the PbNSC in pear fruits.","authors":"Qi Wang, Xinyi Wu, Mei Ren, Fanghang Zhang, Yang Zhang, Yueyang Wang, Wen Li, Zhihua Xie, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Yingying Niu, Shutian Tao","doi":"10.1186/s43897-025-00149-z","DOIUrl":"https://doi.org/10.1186/s43897-025-00149-z","url":null,"abstract":"<p><p>The presence of stone cells in pear fruit, caused by lignified secondary cell walls (SCWs), leads to a grainy texture in the fruit flesh, thereby compromising its overall quality. Lignification is influenced by various environmental signals, including light, however the underlying mechanism are poorly understood. This study reveals that SCW thickening and lignin accumulation in stone cells were regulated by a blue light signal, mediated through the activation of PbNSC by PbbHLH195. The results revealed that the stone cell formation was prompted by supplementary with blue light, with lignin accumulation linked to the upregulation of the NAC STONE CELL PROMOTING FACTOR (PbNSC). PbbHLH195 was identified as a novel molecular hub connecting lignification to blue light signal through its physical interaction with PbCRY1a. The biochemical and functional analysis indicates that PbbHLH195 contributes to stone cell lignification by activating the promoter of PbNSC. Our findings offer novel insights into the mechanisms of lignin biosynthesis in response to blue light, identifying valuable genetic targets for enhancing the fruit quality of pear.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"27"},"PeriodicalIF":10.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histone deacetylases repress the accumulation of licochalcone A by inhibiting the expression of flavonoid biosynthetic pathway-related genes in licorice (Glycyrrhiza inflata). 组蛋白去乙酰化酶通过抑制甘草类黄酮生物合成途径相关基因的表达来抑制甘草查尔酮A的积累。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-06 DOI: 10.1186/s43897-025-00144-4
Jiangyi Zeng, Xiaoling Ma, Yuping Li, Lijun Zhou, Jingxian Fu, Hongxia Wang, Yongliang Liu, Ling Yuan, Ying Wang, Yongqing Li
{"title":"Histone deacetylases repress the accumulation of licochalcone A by inhibiting the expression of flavonoid biosynthetic pathway-related genes in licorice (Glycyrrhiza inflata).","authors":"Jiangyi Zeng, Xiaoling Ma, Yuping Li, Lijun Zhou, Jingxian Fu, Hongxia Wang, Yongliang Liu, Ling Yuan, Ying Wang, Yongqing Li","doi":"10.1186/s43897-025-00144-4","DOIUrl":"https://doi.org/10.1186/s43897-025-00144-4","url":null,"abstract":"<p><p>Histone deacetylases (HDACs) play a crucial role in regulating plant growth, stress responses, and specialized metabolism. Licorice, utilized as both food and herbal medicine for millennia, includes Glycyrrhiza inflata as one of its primary medicinal species used globally. This study investigated the regulatory function of HDAC-mediated histone deacetylation in flavonoid biosynthesis in licorice. The research identified nineteen HDACs in the G. inflata genome. Abiotic stresses and plant hormones were found to influence flavonoid compound accumulation, correlating with altered expression patterns of HDAC genes and global histone H3 acetylation (H3ac) levels. Notably, several HDAC inhibitors enhanced flavonoid accumulation in G. inflata. Subsequent RNA-seq analysis revealed that the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA) activated the expression of multiple genes related to flavonoid biosynthesis. ChIP-qPCR demonstrated that SAHA treatment increased the H3ac levels of flavonoid synthesis-related genes. Furthermore, overexpression of GiHDA2b, an HDAC member, decreased, while RNAi of GiHDA2b increased, the levels of expression and H3K18 acetylation of licochalcone A (LCA) biosynthetic genes indicating its negative role in flavonoid biosynthesis. This research provides valuable insights into the regulatory roles of GiHDACs and histone deacetylation in flavonoid biosynthesis in licorice, potentially contributing to improved bioactive compound production in medicinal plants.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"32"},"PeriodicalIF":10.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Haplotype-resolved genome reveals haplotypic variation and the biosynthesis of medicinal ingredients in Areca catechu L. 单倍型解析基因组揭示槟榔的单倍型变异和药用成分的生物合成。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-02 DOI: 10.1186/s43897-025-00146-2
Chao Wang, Lei Tan, Zhonghui Zhang, Xianggui Li, Linghao Xia, Peng Cao, Haiyang Tong, Xumin Ou, Shixuan Li, Jianing Zhang, Chun Li, Jun Yang, Wen-Biao Jiao, Shouchuang Wang
{"title":"Haplotype-resolved genome reveals haplotypic variation and the biosynthesis of medicinal ingredients in Areca catechu L.","authors":"Chao Wang, Lei Tan, Zhonghui Zhang, Xianggui Li, Linghao Xia, Peng Cao, Haiyang Tong, Xumin Ou, Shixuan Li, Jianing Zhang, Chun Li, Jun Yang, Wen-Biao Jiao, Shouchuang Wang","doi":"10.1186/s43897-025-00146-2","DOIUrl":"https://doi.org/10.1186/s43897-025-00146-2","url":null,"abstract":"<p><p>Areca catechu, as a traditional Chinese medicine, contains a high concentration of therapeutic compounds. However, the biosynthesis of these compounds is largely unexplored. We present a haplotype-resolved genome assembly and annotation for A. catechu, with chromosome-level genome sizes of 2.45 Gb (Ac. Hap1) and 2.49 Gb (Ac. Hap2). A comparative analysis of the haplotypes revealed significant divergence, including multiple Mb-level large inversions. Furthermore, A. catechu shared two whole genome duplications with other palm plants and its genome size had increased due to the insertion of transposons within the last 2.5 million years. By integrating transcriptomics and metabolomics, two tandem genes (AcGNMT1 and AcGNMT2) were negatively associated with guvacine and trigonelline in gene-metabolite interaction network. AcGNMT1, AcGNMT2 and their three homologous genes were involved in the conversion of guvacine to arecoline. Further analyses tested the function of AcUGT71CE15, AcUGT74CJ38, AcUGT87EE5 and AcUGT83S982 as glucosyltransferases, and AcUGT78AP14 was identified as a rhamnosyltransferase involved in flavonol glycosylation. Our study provides a high-quality genome of A. catechu, characterizes the arecoline biosynthetic pathway and expands the understanding of the diversity of UDP-glucosyltransferase and UDP-rhamnosyltransferase, offering insights into the potential of A. catechu for the biosynthesis of bioactive compounds.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"24"},"PeriodicalIF":10.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering the heterogeneous glucosinolates composition in leaves and seeds: strategies for developing Brassica napus genotypes with low seed glucosinolates content but high leaf glucosinolates content. 破译叶片和种子中硫代葡萄糖苷的异质组成:开发种子硫代葡萄糖苷含量低而叶片硫代葡萄糖苷含量高的甘蓝型油菜的策略。
IF 10.6
Molecular Horticulture Pub Date : 2025-05-01 DOI: 10.1186/s43897-025-00147-1
Mengxin Tu, Wenxuan Guan, Antony Maodzeka, Hongyu Zhou, Zi Zhang, Tao Yan, Shuijin Hua, Lixi Jiang
{"title":"Deciphering the heterogeneous glucosinolates composition in leaves and seeds: strategies for developing Brassica napus genotypes with low seed glucosinolates content but high leaf glucosinolates content.","authors":"Mengxin Tu, Wenxuan Guan, Antony Maodzeka, Hongyu Zhou, Zi Zhang, Tao Yan, Shuijin Hua, Lixi Jiang","doi":"10.1186/s43897-025-00147-1","DOIUrl":"https://doi.org/10.1186/s43897-025-00147-1","url":null,"abstract":"<p><p>Rapeseed cakes with low glucosinolates content (GC) possess high feeding value. However, the pursuit of low-GC seeds has inadvertently resulted in a reduction of GC in leaves, making plants more susceptible to stress and lowering their nutritional quality. Therefore, it is imperative to disrupt the tight association between GC in these two tissues and ultimately develop genotypes with low-GC seeds but high-GC leaves. The distinct mechanisms underlying glucosinolate (GSL) synthesis in these two tissues remain unclear. Here, we discovered that aliphatic and aromatic GSLs, rather than indole GSLs, contribute to the positive correlation between GC in seeds and leaves. We performed selective-sweep analyses and identified the genomic footprints left after decades of intense selection for low-GC seeds. By conducting genome-wide association studies and analyzing differentially expressed genes in high- and low-GC seeds and leaves, we compiled lists of distinct genes involved in GSL synthesis in leaves and seeds separately. In particular, BnMYB28 plays a key role in regulating GC in both seeds and leaves. Selection and manipulation of BnaC09.MYB28 would affect GC in both tissues. However, downregulation of BnaA02.MYB28 and/or BnaC02.MYB28 would likely reduce GC in seeds without causing a concurrent reduction in GC in leaves.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"23"},"PeriodicalIF":10.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulation of transcriptional homeostasis by DNA methylation upon genome duplication in pak choi. 小白菜基因组复制时DNA甲基化对转录稳态的调控。
IF 10.6
Molecular Horticulture Pub Date : 2025-04-05 DOI: 10.1186/s43897-025-00145-3
Min Ma, Yuanda Wang, Zhenfei Sun, Ranze Zhao, Honghua Li, Xiaoxuan Li, Hongfang Zhu, Xuedong Yang, Changwei Zhang, Yuda Fang
{"title":"Regulation of transcriptional homeostasis by DNA methylation upon genome duplication in pak choi.","authors":"Min Ma, Yuanda Wang, Zhenfei Sun, Ranze Zhao, Honghua Li, Xiaoxuan Li, Hongfang Zhu, Xuedong Yang, Changwei Zhang, Yuda Fang","doi":"10.1186/s43897-025-00145-3","DOIUrl":"10.1186/s43897-025-00145-3","url":null,"abstract":"<p><p>Polyploidy occurs frequently in plants and is an important force in plant evolution and crop breeding. New polyploids face various challenges due to genome duplication and subsequent changes in epigenetic modifications, nucleus/cell size and gene expression. How polyploids produce evolutionary novelty remains to be understood. In this study, a transcriptome comparison between 21-day-old diploid and autotetraploid pak choi seedlings revealed that there are few differentially expressed genes (DEGs), with a greater proportion of DEGs downregulated in response to genome duplication. Genome-wide DNA methylation analysis indicated that the level of DNA methylation is obviously increased, especially in transposable elements (TEs) and 1 kb flanking regions, upon genome doubling. The differentially methylated regions between diploid and autotetraploid pak choi were related to 12,857 differentially hypermethylated genes and 8,451 hypomethylated genes, and the DEGs were negatively correlated with the differential methylation in the regions across the DEGs. Notably, TE methylation increases significantly in regions flanking neighboring non-DEGs rather than those flanking DEGs. These results shed light on the role of DNA methylation in the transcriptional regulation of genes in polyploids and the mechanism of coping with \"genome shock\" due to genome doubling in cruciferous plants.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"22"},"PeriodicalIF":10.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
AcNAC10, regulated by AcTGA07, enhances kiwifruit resistance to Pseudomonas syringae pv. actinidiae via inhibiting jasmonic acid pathway. AcNAC10由AcTGA07调控,可增强猕猴桃对丁香假单胞菌的抗性。猕猴桃通过抑制茉莉酸途径。
IF 10.6
Molecular Horticulture Pub Date : 2025-04-04 DOI: 10.1186/s43897-024-00143-x
Chao Zhao, Wei Liu, Chenxiao Yao, Yali Zhang, Xiaofei Du, Chao Ma, Rui Li, Hua Wang, Lili Huang
{"title":"AcNAC10, regulated by AcTGA07, enhances kiwifruit resistance to Pseudomonas syringae pv. actinidiae via inhibiting jasmonic acid pathway.","authors":"Chao Zhao, Wei Liu, Chenxiao Yao, Yali Zhang, Xiaofei Du, Chao Ma, Rui Li, Hua Wang, Lili Huang","doi":"10.1186/s43897-024-00143-x","DOIUrl":"10.1186/s43897-024-00143-x","url":null,"abstract":"<p><p>Kiwifruit bacterial canker is a devastating disease caused by Pseudomonas syringae pv. actinidiae (Psa). NAC transcription factors play a significant role in host immunity. However, the potential molecular mechanism of resistance to semi-biotrophic Psa mediated by NAC transcription factors in kiwifruit remains unclear. In this study, we identified a typical NAC transcription factor, AcNAC10, which is involved in the jasmonic acid (JA) pathway and is highly expressed in resistant variety RH12 responsing to Psa. By overexpression and silencing of AcNAC10 in kiwifruit, it plays a positive role in enhancing kiwifruit resistance. Likewise, heterologous expression of AcNAC10 in transgenic Arabidopsis and tomato enhanced resistance to P. syringae. By directly binding to the promoter of AcLOX3, AcNAC10 inhibited its expression as a transcriptional suppressor. Using a yeast one-hybrid screening library, electrophoretic mobility shift assay (EMSA), and dual-luciferase reporter assays, it showed that AcTGA07 can activate the expression of AcNAC10. Moreover, we demonstrated that AcTGA07 decreased JA accumulation independently of the AcNAC10-AcLOX3 pathway. Our study elucidated the transcriptional cascade regulatory network of AcTGA07-AcNAC10-AcLOX3, which enhanced the disease resistance of kiwifruit to Psa by inhibiting JA synthesis.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"21"},"PeriodicalIF":10.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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