Molecular Horticulture最新文献

Apoplastic proteomic reveals Colletotrichum fructicola effector CfXyn11A recognized by tobacco and suppressed by pear in the apoplast. 外质体蛋白质组学揭示了烟草识别、梨抑制的炭疽菌外质体效应因子CfXyn11A。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-07 DOI: 10.1186/s43897-025-00161-3

Chenyang Han, Shutian Tao, Zhihua Xie, Fengquan Liu, Shaoling Zhang

{"title":"Apoplastic proteomic reveals Colletotrichum fructicola effector CfXyn11A recognized by tobacco and suppressed by pear in the apoplast.","authors":"Chenyang Han, Shutian Tao, Zhihua Xie, Fengquan Liu, Shaoling Zhang","doi":"10.1186/s43897-025-00161-3","DOIUrl":"10.1186/s43897-025-00161-3","url":null,"abstract":"Colletotrichum fructicola is a hemibiotrophic fungal plant pathogen that transitions from biotrophic growth on living host tissue to necrotrophic tissue destruction. During the hemibiotrophic phase, numerous proteins are secreted into the apoplast, mediating host‒pathogen interactions. In this study, we employed apoplastic proteomics and RNA-seq to analyse the proteins secreted during the interaction between C. fructicola and pear. A secreted xylanase, CfXyn11A, was identified as a dual-function effector. In the nonhost Nicotiana benthamiana, it triggered immune responses, including reactive oxygen species production and programmed cell death. However, CfXyn11A evades detection in the host pear, enabling its role in cell wall degradation and nutrient acquisition. Genetic and biochemical assays confirmed that the immune-triggering function of CfXyn11A relies on its apoplastic localization and is independent of enzymatic activity. Additionally, we identified an aspartic protease-like protein, PbXIP1, in the pear apoplast, which binds CfXyn11A to suppress its enzymatic activity and virulence. This study highlights the role of apoplastic proteomics in elucidating the molecular mechanisms underlying plant immunity and pathogen virulence and emphasizes the contrasting outcomes of CfXyn11A in different host contexts. The findings provide new insights into the interplay between extracellular effectors and plant defense proteins during fungal infection.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"42"},"PeriodicalIF":10.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PeachMD: a multi-omics database for peach. PeachMD：桃子的多组学数据库。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-04 DOI: 10.1186/s43897-025-00157-z

Ang Li, Shihang Sun, Hongmei Wang, Akhi Badrunnesa, Junren Meng, Xiongwei Li, Yuan Gao, Liang Niu, Lei Pan, Wenyi Duan, Guochao Cui, Zhiqiang Wang, Wenfang Zeng

引用次数: 0

A subgroup I bZIP transcription factor PpbZIP18 plays an important role in sucrose accumulation in peach. bZIP转录因子PpbZIP18亚群在桃的蔗糖积累中起重要作用。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-03 DOI: 10.1186/s43897-025-00156-0

Xian Zhang, Wen Xiao, Yudi Liu, Yunpeng Cao, Ruo-Xi Zhang, Yuepeng Han

引用次数: 0

Breeding a novel cauliflower with exceptional fragrance. 培育出香味独特的新型花椰菜。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-02 DOI: 10.1186/s43897-025-00178-8

Xiaoli Zhang, Xiaoxu Li, Long Chen, Zhenghua Wen, Fengqing Han, Daping Gong, Minmin Xie, Zhe Zhao, Yu Zhao, Wei Zhang, Mingli Chen, Zhiyuan Li

引用次数: 0

Metabolomic and evolutionary integration unveils medicinal potential in six Corydalis species. 代谢组学和进化整合揭示了六种延胡索属植物的药用潜力。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-01 DOI: 10.1186/s43897-025-00162-2

Yun Gao, Xiangyu Zhou, Mengxiao Yan, Zhengwei Wang, Xin Zhong, Xiaochen Li, Junjie Zhu, Yu Kong, Wanrong Zhu, Ruolin Geng, Yaping Zhou, Qing Zhao, Yonghong Hu, Ping Xu

{"title":"Metabolomic and evolutionary integration unveils medicinal potential in six Corydalis species.","authors":"Yun Gao, Xiangyu Zhou, Mengxiao Yan, Zhengwei Wang, Xin Zhong, Xiaochen Li, Junjie Zhu, Yu Kong, Wanrong Zhu, Ruolin Geng, Yaping Zhou, Qing Zhao, Yonghong Hu, Ping Xu","doi":"10.1186/s43897-025-00162-2","DOIUrl":"10.1186/s43897-025-00162-2","url":null,"abstract":"The Corydalis genus, one of the largest within the Papaveraceae family, holds a rich diversity of medicinal resources, particularly among its tuberous species. Benzylisoquinoline alkaloids (BIAs) are primarily responsible for the medicinal properties observed in Corydalis plants. In this study, we conducted an integrated evolutionary analysis by combining whole-genome resequencing with comprehensive metabolite profiling across various Corydalis species. Guided by these initial findings, supported by local cultivation practices and an extensive literature review, we further investigated six tuberous Corydalis species: C. yanhusuo, C. decumbens, C. schanginii, C. ledebouriana, C. solida, and the newly identified C. nanchuanensis. Our results revealed conserved alkaloid profiles across these species but highlighted significant variations in key bioactive compounds. Notably, C. nanchuanensis exhibited considerably higher levels of tetrahydropalmatine compared to the commonly used medicinal species C. yanhusuo, while C. solida, originally sourced from the Netherlands, displayed elevated concentrations of corydaline, palmatine, and dehydrocorydaline. Additionally, transcriptome-metabolome correlation analyses pinpointed several critical genes involved in protopine biosynthesis, particularly emphasizing the TNMT gene family. These discoveries significantly enhance our understanding of metabolic diversity in tuberous Corydalis, providing essential insights for the exploration of novel medicinal resources and facilitating targeted genetic improvements for therapeutic use.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"38"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12211431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening. 肉质果实成熟过程中显性激素信号产生的触发机制。

IF 10.6

Molecular Horticulture Pub Date : 2025-06-06 DOI: 10.1186/s43897-025-00155-1

Jinyao Ouyang, Bing He, Ya Zeng, Changsheng Zhai, Yating Li, Jie Li, Pingyin Guan, Wensuo Jia

{"title":"The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening.","authors":"Jinyao Ouyang, Bing He, Ya Zeng, Changsheng Zhai, Yating Li, Jie Li, Pingyin Guan, Wensuo Jia","doi":"10.1186/s43897-025-00155-1","DOIUrl":"10.1186/s43897-025-00155-1","url":null,"abstract":"Fleshy Fruit (FF) ripening is regulated by multiple hormones, which can be categorized into two groups, i.e., the positive signals acting to promote FF ripening and the negative signals acting to suppress FF ripening. Ethylene (ET) and abscisic acid (ABA) are two predominant positive signals respectively controlling climacteric (CL) and non-climacteric (NC) FF ripening, whereas auxin (IAA) is the predominant negative signal controlling both FF growth and ripening. Functioning of these hormones is initiated by an alteration of the hormonal levels, which is referred to as the process of Hormonal Signal Production (HSP) in FF development and ripening. While the hormonal regulation of FF ripening has been extensively studied and reviewed, knowledge of HSP has never been summarized and discussed. The purpose of this review is to summarize and discuss the triggering mechanism of HSP. We first summarize the physiological, biochemical and molecular bases of HSP for three crucial hormones, ET, ABA, and IAA, including hormonal metabolism, transport and reciprocal regulation of HSP among different hormones, we then summarize and discuss the recent discoveries on the mechanism of cellular signal transduction of HSP. Finally, we propose several viewpoints to facilitate comprehension of the future research endeavors.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"35"},"PeriodicalIF":10.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smi-miRmTERF regulates organelle development, retrograde signaling, secondary metabolism and immunity via targeting a subset of SmmTERFs in Salvia miltiorrhiza. Smi-miRmTERF通过靶向丹参中smmterf的一个亚群调节细胞器发育、逆行信号、次生代谢和免疫。

IF 10.6

Molecular Horticulture Pub Date : 2025-06-05 DOI: 10.1186/s43897-025-00153-3

Xiaoxiao Qiu, Hong Zhou, Jiang Li, Miaomiao Liu, Xian Pan, Butuo Zhu, Sixuan Zhang, Fanqi Meng, Caili Li, Shanfa Lu

{"title":"Smi-miRmTERF regulates organelle development, retrograde signaling, secondary metabolism and immunity via targeting a subset of SmmTERFs in Salvia miltiorrhiza.","authors":"Xiaoxiao Qiu, Hong Zhou, Jiang Li, Miaomiao Liu, Xian Pan, Butuo Zhu, Sixuan Zhang, Fanqi Meng, Caili Li, Shanfa Lu","doi":"10.1186/s43897-025-00153-3","DOIUrl":"10.1186/s43897-025-00153-3","url":null,"abstract":"MicroRNAs are a class of endogenous small non-coding RNAs, some of which can trigger phased secondary small interfering RNA (phasiRNA) production from target genes. Mitochondrial transcription termination factors (mTERFs), mainly localized in chloroplasts and/or mitochondria, play critical roles in plant development and stress responses. We report here the identification of 63 mTERFs and a 22 nt novel miRNA (smi-miRmTERF), which directly cleave SmmTERF33 and SmmTERF45 transcripts to trigger phasiRNA biogenesis. The generated phasiRNAs could further trigger phasiRNA biogenesis from SmmTERF26 and regulated a subset of lineage-specific SmmTERFs. MIRmTERF widely existed in Nepetoideae plants and SmmTERF33 and SmmTERF45 proteins were localized in chloroplasts, mitochondria, and the cytoplasm. Smi-miRmTERF overexpression (MIRMTERF#OE) resulted in a dwarfing phenotype with severe defects in chloroplast and mitochondrial morphogenesis. Transcriptomic analysis showed up-regulation of defense-related and down-regulation of photosynthesis-related genes in MIRMTERF#OE plants. Under norflurazon and lincomycin treatments, MIRMTERF#OE plants displayed a gun phenotype, indicating the role of smi-miRmTERF in retrograde signaling. Furthermore, MIRMTERF#OE plants showed increased contents of phenolic acids, monoterpenoids, and sesquiterpenoids and reduced susceptibility to pathogenic bacteria Pst DC3000. The results suggest that smi-miRmTERF is a significant regulator of chloroplast and mitochondrial development, retrograde signaling, secondary metabolism, and immunity in S. miltiorrhiza.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"34"},"PeriodicalIF":10.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family. 山毛榉栎家族花、果发育及NLR基因的进化。

IF 10.6

Molecular Horticulture Pub Date : 2025-06-04 DOI: 10.1186/s43897-025-00152-4

Xiong-De Tu, Wen-Jun Lin, Ya-Xuan Xin, Hou-Hua Fu, Cheng-Yuan Zhou, Yi-Zhe Lin, Jun Shen, Shuai Chen, Hui Lian, Shu-Zhen Jiang, Bin Liu, Yu Li, Zi Wang, Ding-Kun Liu, Zhi-Wen Wang, Siren Lan, Ming-He Li, Zhong-Jian Liu, Shi-Pin Chen

{"title":"Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family.","authors":"Xiong-De Tu, Wen-Jun Lin, Ya-Xuan Xin, Hou-Hua Fu, Cheng-Yuan Zhou, Yi-Zhe Lin, Jun Shen, Shuai Chen, Hui Lian, Shu-Zhen Jiang, Bin Liu, Yu Li, Zi Wang, Ding-Kun Liu, Zhi-Wen Wang, Siren Lan, Ming-He Li, Zhong-Jian Liu, Shi-Pin Chen","doi":"10.1186/s43897-025-00152-4","DOIUrl":"10.1186/s43897-025-00152-4","url":null,"abstract":"The Fagaceae family, comprising over 900 species, is an essential component of Northern Hemisphere forest ecosystems. However, genomic data for tropical and subtropical genera Castanopsis and Castanea remain limited compared to the well-studied oak. Here, we present chromosome-level genome assemblies of Castanopsis carlesii and Castanea henryi, with assembled genome sizes of 927.24 Mb (N50 = 1.57 Mb) and 780.10 Mb (N50 = 1.07 Mb), respectively, and repetitive sequence contents of 45.79% and 44.88%. Comparative genomic analysis revealed that the estimated divergence time between Castanopsis and Castanea was determined to be 48.3 Mya and provided evidence that both genera experienced only one of the ancient whole genome triplication event (γ event) shared with most eudicots. The development of C. carlesii flower bracts and cupules was controlled by A- and E-class genes, suggesting that the cupules may originate from the bracts. Additionally, genes involved in sucrose and starch metabolism genes played distinct roles during C. carlesii fruit development. The amplification of the nucleotide-binding leucine-rich repeat (NLR) gene family in Fagaceae exhibited similarities, indicating that this expansion may be an adaptation to similar environmental pressures. This study provides valuable genomic resources for Asian Fagaceae and enhances our understanding of Fagaceae evolution.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"33"},"PeriodicalIF":10.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12135283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A native visual screening reporter-assisted CRISPR/Cas9 system for high-efficient genome editing in strawberry. 一种用于草莓高效基因组编辑的原生目视筛选报告器辅助CRISPR/Cas9系统

IF 10.6

Molecular Horticulture Pub Date : 2025-06-03 DOI: 10.1186/s43897-025-00151-5

Xianyan Han, Xia Liang, Dongdong Li, Miaoying Song, Zhimin Ma, Ruixia Li, Han Meng, Yue Cai, Bailong Song, Zhongchi Liu, Houcheng Zhou, Junhui Zhou

引用次数: 0

Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes. 多组学分析揭示了控制四种不同西瓜果肉颜色表型的双重遗传位点。

IF 10.6

Molecular Horticulture Pub Date : 2025-05-14 DOI: 10.1186/s43897-025-00166-y

Na Li, Shilai Xing, Gaofei Sun, Jianli Shang, Jia-Long Yao, Nannan Li, Dan Zhou, Yu Wang, Yuan Lu, Jinpeng Bi, Jiming Wang, Hongfeng Lu, Shuangwu Ma

{"title":"Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes.","authors":"Na Li, Shilai Xing, Gaofei Sun, Jianli Shang, Jia-Long Yao, Nannan Li, Dan Zhou, Yu Wang, Yuan Lu, Jinpeng Bi, Jiming Wang, Hongfeng Lu, Shuangwu Ma","doi":"10.1186/s43897-025-00166-y","DOIUrl":"10.1186/s43897-025-00166-y","url":null,"abstract":"Watermelon fruit flesh displays various colors. Although genetic loci underlying these variations are identified, the molecular mechanism remains elusive. Here, we assembled a chromosome-scale reference genome of an elite watermelon and developed integrated genetic maps using single nucleotide polymorphism (SNP) and structural variation markers. Several key genetic varients for fruit shape and flesh color were identified. Two variants associated with flesh color were further studied, including one copy number variant (CNV, a triplicate of 1.2 kb DNA) in the promoter region of REDUCED CHLOROPLAST COVERAGE 2 (ClREC2) and one SNP in Lycopene β-Cyclase (ClLCYB) coding region. These two variants together explained 99.7% of the flesh color variations in 314 watermelon accessions. The SNP in ClLCYB was the same as previously reported, disrupting ClLCYB function. The CNV could strongly enhance ClREC2 expression, consequently increasing the expression of carotenoid biosynthesis genes, the number of plastoglobules within chromoplasts, and carotenoid level in mature fruit flesh. Finally, we proposed a \"two-switch\" genetic model by integrating two major causative loci, which can explain the formation of the four main flesh colors in different watermelon accessions. These results provide new insights into the regulation of carotenoid biosynthesis and color formation in plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"46"},"PeriodicalIF":10.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0