Molecular Horticulture最新文献

The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening. 肉质果实成熟过程中显性激素信号产生的触发机制。

IF 10.6

Molecular Horticulture Pub Date : 2025-06-06 DOI: 10.1186/s43897-025-00155-1

Jinyao Ouyang, Bing He, Ya Zeng, Changsheng Zhai, Yating Li, Jie Li, Pingyin Guan, Wensuo Jia

{"title":"The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening.","authors":"Jinyao Ouyang, Bing He, Ya Zeng, Changsheng Zhai, Yating Li, Jie Li, Pingyin Guan, Wensuo Jia","doi":"10.1186/s43897-025-00155-1","DOIUrl":"10.1186/s43897-025-00155-1","url":null,"abstract":"Fleshy Fruit (FF) ripening is regulated by multiple hormones, which can be categorized into two groups, i.e., the positive signals acting to promote FF ripening and the negative signals acting to suppress FF ripening. Ethylene (ET) and abscisic acid (ABA) are two predominant positive signals respectively controlling climacteric (CL) and non-climacteric (NC) FF ripening, whereas auxin (IAA) is the predominant negative signal controlling both FF growth and ripening. Functioning of these hormones is initiated by an alteration of the hormonal levels, which is referred to as the process of Hormonal Signal Production (HSP) in FF development and ripening. While the hormonal regulation of FF ripening has been extensively studied and reviewed, knowledge of HSP has never been summarized and discussed. The purpose of this review is to summarize and discuss the triggering mechanism of HSP. We first summarize the physiological, biochemical and molecular bases of HSP for three crucial hormones, ET, ABA, and IAA, including hormonal metabolism, transport and reciprocal regulation of HSP among different hormones, we then summarize and discuss the recent discoveries on the mechanism of cellular signal transduction of HSP. Finally, we propose several viewpoints to facilitate comprehension of the future research endeavors.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"35"},"PeriodicalIF":10.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smi-miRmTERF regulates organelle development, retrograde signaling, secondary metabolism and immunity via targeting a subset of SmmTERFs in Salvia miltiorrhiza. Smi-miRmTERF通过靶向丹参中smmterf的一个亚群调节细胞器发育、逆行信号、次生代谢和免疫。

IF 10.6

Molecular Horticulture Pub Date : 2025-06-05 DOI: 10.1186/s43897-025-00153-3

Xiaoxiao Qiu, Hong Zhou, Jiang Li, Miaomiao Liu, Xian Pan, Butuo Zhu, Sixuan Zhang, Fanqi Meng, Caili Li, Shanfa Lu

{"title":"Smi-miRmTERF regulates organelle development, retrograde signaling, secondary metabolism and immunity via targeting a subset of SmmTERFs in Salvia miltiorrhiza.","authors":"Xiaoxiao Qiu, Hong Zhou, Jiang Li, Miaomiao Liu, Xian Pan, Butuo Zhu, Sixuan Zhang, Fanqi Meng, Caili Li, Shanfa Lu","doi":"10.1186/s43897-025-00153-3","DOIUrl":"10.1186/s43897-025-00153-3","url":null,"abstract":"MicroRNAs are a class of endogenous small non-coding RNAs, some of which can trigger phased secondary small interfering RNA (phasiRNA) production from target genes. Mitochondrial transcription termination factors (mTERFs), mainly localized in chloroplasts and/or mitochondria, play critical roles in plant development and stress responses. We report here the identification of 63 mTERFs and a 22 nt novel miRNA (smi-miRmTERF), which directly cleave SmmTERF33 and SmmTERF45 transcripts to trigger phasiRNA biogenesis. The generated phasiRNAs could further trigger phasiRNA biogenesis from SmmTERF26 and regulated a subset of lineage-specific SmmTERFs. MIRmTERF widely existed in Nepetoideae plants and SmmTERF33 and SmmTERF45 proteins were localized in chloroplasts, mitochondria, and the cytoplasm. Smi-miRmTERF overexpression (MIRMTERF#OE) resulted in a dwarfing phenotype with severe defects in chloroplast and mitochondrial morphogenesis. Transcriptomic analysis showed up-regulation of defense-related and down-regulation of photosynthesis-related genes in MIRMTERF#OE plants. Under norflurazon and lincomycin treatments, MIRMTERF#OE plants displayed a gun phenotype, indicating the role of smi-miRmTERF in retrograde signaling. Furthermore, MIRMTERF#OE plants showed increased contents of phenolic acids, monoterpenoids, and sesquiterpenoids and reduced susceptibility to pathogenic bacteria Pst DC3000. The results suggest that smi-miRmTERF is a significant regulator of chloroplast and mitochondrial development, retrograde signaling, secondary metabolism, and immunity in S. miltiorrhiza.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"34"},"PeriodicalIF":10.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family. 山毛榉栎家族花、果发育及NLR基因的进化。

IF 10.6

Molecular Horticulture Pub Date : 2025-06-04 DOI: 10.1186/s43897-025-00152-4

Xiong-De Tu, Wen-Jun Lin, Ya-Xuan Xin, Hou-Hua Fu, Cheng-Yuan Zhou, Yi-Zhe Lin, Jun Shen, Shuai Chen, Hui Lian, Shu-Zhen Jiang, Bin Liu, Yu Li, Zi Wang, Ding-Kun Liu, Zhi-Wen Wang, Siren Lan, Ming-He Li, Zhong-Jian Liu, Shi-Pin Chen

{"title":"Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family.","authors":"Xiong-De Tu, Wen-Jun Lin, Ya-Xuan Xin, Hou-Hua Fu, Cheng-Yuan Zhou, Yi-Zhe Lin, Jun Shen, Shuai Chen, Hui Lian, Shu-Zhen Jiang, Bin Liu, Yu Li, Zi Wang, Ding-Kun Liu, Zhi-Wen Wang, Siren Lan, Ming-He Li, Zhong-Jian Liu, Shi-Pin Chen","doi":"10.1186/s43897-025-00152-4","DOIUrl":"10.1186/s43897-025-00152-4","url":null,"abstract":"The Fagaceae family, comprising over 900 species, is an essential component of Northern Hemisphere forest ecosystems. However, genomic data for tropical and subtropical genera Castanopsis and Castanea remain limited compared to the well-studied oak. Here, we present chromosome-level genome assemblies of Castanopsis carlesii and Castanea henryi, with assembled genome sizes of 927.24 Mb (N50 = 1.57 Mb) and 780.10 Mb (N50 = 1.07 Mb), respectively, and repetitive sequence contents of 45.79% and 44.88%. Comparative genomic analysis revealed that the estimated divergence time between Castanopsis and Castanea was determined to be 48.3 Mya and provided evidence that both genera experienced only one of the ancient whole genome triplication event (γ event) shared with most eudicots. The development of C. carlesii flower bracts and cupules was controlled by A- and E-class genes, suggesting that the cupules may originate from the bracts. Additionally, genes involved in sucrose and starch metabolism genes played distinct roles during C. carlesii fruit development. The amplification of the nucleotide-binding leucine-rich repeat (NLR) gene family in Fagaceae exhibited similarities, indicating that this expansion may be an adaptation to similar environmental pressures. This study provides valuable genomic resources for Asian Fagaceae and enhances our understanding of Fagaceae evolution.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"33"},"PeriodicalIF":10.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12135283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A native visual screening reporter-assisted CRISPR/Cas9 system for high-efficient genome editing in strawberry. 一种用于草莓高效基因组编辑的原生目视筛选报告器辅助CRISPR/Cas9系统

IF 10.6

Molecular Horticulture Pub Date : 2025-06-03 DOI: 10.1186/s43897-025-00151-5

Xianyan Han, Xia Liang, Dongdong Li, Miaoying Song, Zhimin Ma, Ruixia Li, Han Meng, Yue Cai, Bailong Song, Zhongchi Liu, Houcheng Zhou, Junhui Zhou

引用次数: 0

Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes. 多组学分析揭示了控制四种不同西瓜果肉颜色表型的双重遗传位点。

IF 10.6

Molecular Horticulture Pub Date : 2025-05-14 DOI: 10.1186/s43897-025-00166-y

Na Li, Shilai Xing, Gaofei Sun, Jianli Shang, Jia-Long Yao, Nannan Li, Dan Zhou, Yu Wang, Yuan Lu, Jinpeng Bi, Jiming Wang, Hongfeng Lu, Shuangwu Ma

{"title":"Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes.","authors":"Na Li, Shilai Xing, Gaofei Sun, Jianli Shang, Jia-Long Yao, Nannan Li, Dan Zhou, Yu Wang, Yuan Lu, Jinpeng Bi, Jiming Wang, Hongfeng Lu, Shuangwu Ma","doi":"10.1186/s43897-025-00166-y","DOIUrl":"10.1186/s43897-025-00166-y","url":null,"abstract":"Watermelon fruit flesh displays various colors. Although genetic loci underlying these variations are identified, the molecular mechanism remains elusive. Here, we assembled a chromosome-scale reference genome of an elite watermelon and developed integrated genetic maps using single nucleotide polymorphism (SNP) and structural variation markers. Several key genetic varients for fruit shape and flesh color were identified. Two variants associated with flesh color were further studied, including one copy number variant (CNV, a triplicate of 1.2 kb DNA) in the promoter region of REDUCED CHLOROPLAST COVERAGE 2 (ClREC2) and one SNP in Lycopene β-Cyclase (ClLCYB) coding region. These two variants together explained 99.7% of the flesh color variations in 314 watermelon accessions. The SNP in ClLCYB was the same as previously reported, disrupting ClLCYB function. The CNV could strongly enhance ClREC2 expression, consequently increasing the expression of carotenoid biosynthesis genes, the number of plastoglobules within chromoplasts, and carotenoid level in mature fruit flesh. Finally, we proposed a \"two-switch\" genetic model by integrating two major causative loci, which can explain the formation of the four main flesh colors in different watermelon accessions. These results provide new insights into the regulation of carotenoid biosynthesis and color formation in plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"46"},"PeriodicalIF":10.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new climate for genomic and epigenomic innovation in grapevine. 葡萄基因组学和表观基因组学创新的新气象。

IF 10.6

Molecular Horticulture Pub Date : 2025-05-12 DOI: 10.1186/s43897-025-00171-1

Maximilian Schmidt, Timo Strack, Haylie Andrews, Lee T Hickey, Peter A Crisp, Kai P Voss-Fels

引用次数: 0

ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more. 橙家族蛋白：多功能伴侣塑造植物类胡萝卜素水平，质体发育，抗逆性等。

IF 10.6

Molecular Horticulture Pub Date : 2025-05-09 DOI: 10.1186/s43897-025-00169-9

Emalee Wrightstone, Lilin Xu, Sombir Rao, Abhijit Hazra, Li Li

{"title":"ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more.","authors":"Emalee Wrightstone, Lilin Xu, Sombir Rao, Abhijit Hazra, Li Li","doi":"10.1186/s43897-025-00169-9","DOIUrl":"https://doi.org/10.1186/s43897-025-00169-9","url":null,"abstract":"ORANGE (OR) family proteins are DnaJE1 molecular chaperones ubiquitous and highly conserved in all plant species, indicating their important roles in plant growth and development. OR proteins have been found to exert multiple functions in regulating carotenoid and chlorophyll biosynthesis, plastid development, and stress tolerance, with additional functions expected to be discovered. As molecular chaperones, OR proteins directly influence the stability of their target proteins via their holdase activity and may perform other molecular roles through unknown mechanisms. Exploration of OR has uncovered novel mechanisms underlying core plant metabolism pathways and expanded our understanding of processes linked to plastid development. Continued investigation of OR family proteins will not only reveal new functions of molecular chaperones but also provide pioneering tools for crop improvement. Thus, OR family proteins offer a distinctive opportunity to comprehend molecular chaperones in modulating various metabolic and developmental processes and exemplify the importance of chaperones in crop development and adaptability. This review briefly details the history of OR family proteins, highlights recent advancements in understanding their myriad of functions, and discusses the prospects of this fascinating group of chaperones towards generating innovative, more nutritious, and resilient crops alongside other agronomically important traits.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"43"},"PeriodicalIF":10.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C2H2-type zinc finger protein transcription factor MdZAT1 plays a negative role in anthocyanin biosynthesis in apple. c2h2型锌指蛋白转录因子MdZAT1在苹果花青素生物合成中起负向作用。

IF 10.6

Molecular Horticulture Pub Date : 2025-05-08 DOI: 10.1186/s43897-025-00150-6

Yanxue Ren, Wenping Huo, Zhongkang Wang, Shasha Liu, Yizhou Chen, Xiaolong Xu, Hongmin Hou, Chaohua Dong, Jihua Xu, Min Chen, Yugang Zhang, Shenghui Jiang

引用次数: 0

Cryptochrome-mediated blue light regulates cell lignification via PbbHLH195 activation of the PbNSC in pear fruits. 隐色素介导的蓝光通过PbbHLH195激活梨果实PbNSC调控细胞木质化。

IF 10.6

Molecular Horticulture Pub Date : 2025-05-07 DOI: 10.1186/s43897-025-00149-z

Qi Wang, Xinyi Wu, Mei Ren, Fanghang Zhang, Yang Zhang, Yueyang Wang, Wen Li, Zhihua Xie, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Yingying Niu, Shutian Tao

{"title":"Cryptochrome-mediated blue light regulates cell lignification via PbbHLH195 activation of the PbNSC in pear fruits.","authors":"Qi Wang, Xinyi Wu, Mei Ren, Fanghang Zhang, Yang Zhang, Yueyang Wang, Wen Li, Zhihua Xie, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Yingying Niu, Shutian Tao","doi":"10.1186/s43897-025-00149-z","DOIUrl":"https://doi.org/10.1186/s43897-025-00149-z","url":null,"abstract":"The presence of stone cells in pear fruit, caused by lignified secondary cell walls (SCWs), leads to a grainy texture in the fruit flesh, thereby compromising its overall quality. Lignification is influenced by various environmental signals, including light, however the underlying mechanism are poorly understood. This study reveals that SCW thickening and lignin accumulation in stone cells were regulated by a blue light signal, mediated through the activation of PbNSC by PbbHLH195. The results revealed that the stone cell formation was prompted by supplementary with blue light, with lignin accumulation linked to the upregulation of the NAC STONE CELL PROMOTING FACTOR (PbNSC). PbbHLH195 was identified as a novel molecular hub connecting lignification to blue light signal through its physical interaction with PbCRY1a. The biochemical and functional analysis indicates that PbbHLH195 contributes to stone cell lignification by activating the promoter of PbNSC. Our findings offer novel insights into the mechanisms of lignin biosynthesis in response to blue light, identifying valuable genetic targets for enhancing the fruit quality of pear.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"27"},"PeriodicalIF":10.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone deacetylases repress the accumulation of licochalcone A by inhibiting the expression of flavonoid biosynthetic pathway-related genes in licorice (Glycyrrhiza inflata). 组蛋白去乙酰化酶通过抑制甘草类黄酮生物合成途径相关基因的表达来抑制甘草查尔酮A的积累。

IF 10.6

Molecular Horticulture Pub Date : 2025-05-06 DOI: 10.1186/s43897-025-00144-4

Jiangyi Zeng, Xiaoling Ma, Yuping Li, Lijun Zhou, Jingxian Fu, Hongxia Wang, Yongliang Liu, Ling Yuan, Ying Wang, Yongqing Li

{"title":"Histone deacetylases repress the accumulation of licochalcone A by inhibiting the expression of flavonoid biosynthetic pathway-related genes in licorice (Glycyrrhiza inflata).","authors":"Jiangyi Zeng, Xiaoling Ma, Yuping Li, Lijun Zhou, Jingxian Fu, Hongxia Wang, Yongliang Liu, Ling Yuan, Ying Wang, Yongqing Li","doi":"10.1186/s43897-025-00144-4","DOIUrl":"https://doi.org/10.1186/s43897-025-00144-4","url":null,"abstract":"Histone deacetylases (HDACs) play a crucial role in regulating plant growth, stress responses, and specialized metabolism. Licorice, utilized as both food and herbal medicine for millennia, includes Glycyrrhiza inflata as one of its primary medicinal species used globally. This study investigated the regulatory function of HDAC-mediated histone deacetylation in flavonoid biosynthesis in licorice. The research identified nineteen HDACs in the G. inflata genome. Abiotic stresses and plant hormones were found to influence flavonoid compound accumulation, correlating with altered expression patterns of HDAC genes and global histone H3 acetylation (H3ac) levels. Notably, several HDAC inhibitors enhanced flavonoid accumulation in G. inflata. Subsequent RNA-seq analysis revealed that the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA) activated the expression of multiple genes related to flavonoid biosynthesis. ChIP-qPCR demonstrated that SAHA treatment increased the H3ac levels of flavonoid synthesis-related genes. Furthermore, overexpression of GiHDA2b, an HDAC member, decreased, while RNAi of GiHDA2b increased, the levels of expression and H3K18 acetylation of licochalcone A (LCA) biosynthetic genes indicating its negative role in flavonoid biosynthesis. This research provides valuable insights into the regulatory roles of GiHDACs and histone deacetylation in flavonoid biosynthesis in licorice, potentially contributing to improved bioactive compound production in medicinal plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"32"},"PeriodicalIF":10.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0