Molecular Horticulture最新文献

Correction: Efficient genome editing in grapevine using CRISPR/LbCas12a system.

IF 10.6

Molecular Horticulture Pub Date : 2024-12-13 DOI: 10.1186/s43897-024-00133-z

Chong Ren, Elias Kirabi Gathunga, Xue Li, Huayang Li, Junhua Kong, Zhanwu Dai, Zhenchang Liang

引用次数: 0

Potato steroidal glycoalkaloids: properties, biosynthesis, regulation and genetic manipulation.

IF 10.6

Molecular Horticulture Pub Date : 2024-12-13 DOI: 10.1186/s43897-024-00118-y

Yongming Liu, Xiaowei Liu, Yingge Li, Yanfei Pei, Abdul Jaleel, Maozhi Ren

引用次数: 0

Recent advances and future directions in banana molecular biology and breeding.

IF 10.6

Molecular Horticulture Pub Date : 2024-12-02 DOI: 10.1186/s43897-024-00122-2

Chunzhen Cheng, Shuofan Wu, Guiming Deng, Ou Sheng, Ganjun Yi, Qiaosong Yang

引用次数: 0

Horizontal transfer of plasmid-like extrachromosomal circular DNAs across graft junctions in Solanaceae. 茄科植物中类似质粒的染色体外环状 DNA 跨嫁接接头的水平转移。

IF 10.6

Molecular Horticulture Pub Date : 2024-11-20 DOI: 10.1186/s43897-024-00124-0

Aijun Zhang, Tingjin Wang, Lu Yuan, Yuxin Shen, Ke Liu, Bin Liu, Kexin Xu, Mohamed A Elsadek, Yiting Wang, Liang Wu, Zhenyu Qi, Jingquan Yu, Mingfang Zhang, Liping Chen

{"title":"Horizontal transfer of plasmid-like extrachromosomal circular DNAs across graft junctions in Solanaceae.","authors":"Aijun Zhang, Tingjin Wang, Lu Yuan, Yuxin Shen, Ke Liu, Bin Liu, Kexin Xu, Mohamed A Elsadek, Yiting Wang, Liang Wu, Zhenyu Qi, Jingquan Yu, Mingfang Zhang, Liping Chen","doi":"10.1186/s43897-024-00124-0","DOIUrl":"10.1186/s43897-024-00124-0","url":null,"abstract":"The transfer of genetic material between stocks and scions of grafted plants has been extensively studied; however, the nature and frequency of the transferred material remain elusive. Here, we report a grafting system involving woody goji as the stock and herbaceous tomato as the scion, which was developed using in vitro and in vivo approaches; the results confirmed horizontal transfer of multiple nuclear DNA fragments from donor goji cells to recipient tomato cells. Tomato tissues containing goji donor DNA fragments at or near the grafting junctions had a perennial-biased anatomical structure, from which roots or shoots were regenerated. Most of the fragments were plasmid-like extrachromosomal circular DNAs (eccDNAs) present in the regenerants derived from the cells and in their asexual offspring. Plants with transferred eccDNAs in regenerated roots or shoots (designated \"Go-tomato\") were grown perennially and showed excellent agronomic performance. The present study provides new insights into the replication, expression, and potential function of eccDNAs in the pleiotropic traits of Go-tomato. Mobile eccDNAs offer evidence of stock-to-scion horizontal DNA transfer beyond chromosomes and organelles, thereby contributing to the molecular understanding of graft-induced genetic variation, evolution, and breeding.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"4 1","pages":"41"},"PeriodicalIF":10.6,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11577957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor PbrERF114 is involved in the regulation of ethylene synthesis during pear fruit ripening. 转录因子 PbrERF114 参与了梨果成熟过程中乙烯合成的调控。

IF 10.6

Molecular Horticulture Pub Date : 2024-11-15 DOI: 10.1186/s43897-024-00114-2

Guoming Wang, Zhihua Guo, Tengjiao Wang, Xueping Wang, Kaijie Qi, Jiping Xuan, Chao Gu, Shaoling Zhang

{"title":"Transcription factor PbrERF114 is involved in the regulation of ethylene synthesis during pear fruit ripening.","authors":"Guoming Wang, Zhihua Guo, Tengjiao Wang, Xueping Wang, Kaijie Qi, Jiping Xuan, Chao Gu, Shaoling Zhang","doi":"10.1186/s43897-024-00114-2","DOIUrl":"10.1186/s43897-024-00114-2","url":null,"abstract":"The plant hormone ethylene is indispensable to the ripening of climacteric fruits. Although extensive studies have been conducted on ethylene signaling, the ethylene response factor (ERF)-mediated transcriptional regulation of ethylene biosynthesis in pear fruits remains to be fully elucidated. We here constructed, sequenced, and analyzed transcriptome libraries in ethephon-treated and 1-MCP-treated Cuiguan pear fruits. In total, 721 fruit ripening-associated differentially expressed genes were identified. Among them, two key genes exhibited positive correlations: the 1-aminocyclopropane-1-carboxylic acid synthase (ACS)-encoding gene PbrACS3 and the ERF-encoding gene named PbrERF114. PbrERF114 overexpression increased ethylene production as well as the PbrACS3 expression level. Conversely, virus-induced gene silencing downregulated PbrERF114, thereby decreasing ethylene production and reducing PbrACS3 expression levels. Notably, PbrERF114 could directly interact with PbrACS3 and PbrERF24 promoters, thus inducing their expression. However, it did not result in an enhancement in luciferase activity, which is regulated by the PbrACS1b or PbrACO1 promoter. PbrERF24 could directly bind to PbrACO1 as well as PbrACS3 to promote ethylene synthesis. In conclusion, PbrERF114 can directly and indirectly mediate ethylene biosynthesis by transcriptionally regulating PbrACS3 and PbrERF24, respectively, thereby triggering a signaling cascade that induces the expression of both PbrACS3 and PbrACO1.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"4 1","pages":"38"},"PeriodicalIF":10.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Begomoviruses associated with okra yellow vein mosaic disease (OYVMD): diversity, transmission mechanism, and management strategies. 与黄秋葵黄脉花叶病（OYVMD）相关的蚕豆病毒：多样性、传播机制和管理策略。

IF 10.6

Molecular Horticulture Pub Date : 2024-11-05 DOI: 10.1186/s43897-024-00112-4

Thomas Wilbur Davis, Andrew Nasa Thompson

{"title":"Begomoviruses associated with okra yellow vein mosaic disease (OYVMD): diversity, transmission mechanism, and management strategies.","authors":"Thomas Wilbur Davis, Andrew Nasa Thompson","doi":"10.1186/s43897-024-00112-4","DOIUrl":"10.1186/s43897-024-00112-4","url":null,"abstract":"Okra yellow vein mosaic disease (OYVMD) is a major constraint to okra production globally. It is caused by several distinct begomoviruses, including okra yellow vein mosaic virus (OYVMV), that are transmitted by the whitefly. This study synthesizes current knowledge on the complex interactions between whiteflies, begomoviruses, and okra plants that enable viral spread and cause OYVMD. The acquisition and transmission cycle involves specific processes including virion ingestion during phloem-feeding, endocytosis and passage across insect tissues, secretion in saliva, and inoculation into plants. Molecular compatibilities between vector coat proteins, midgut proteins, and plant factors modulate virus replication and movement through barrier tissues. Abiotic stresses and host traits also impact whitefly behavior and virus epidemiology. Begomoviruses such as OYVMV have spread globally wherever whitefly vectors and susceptible okra varieties occur. Integrated management of the tripartite pathosystem that incorporates host resistance, cultural tactics, and biological control is required to mitigate the transmission of begomoviruses and OYVMD impact. Finally, resolving vector-virus interactions and developing interference strategies will help contribute to strengthening okra germplasm resistance which can support sustainable food production.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"4 1","pages":"36"},"PeriodicalIF":10.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture. VvD14c-VvMAX2-VvLOB/VvLBD19模块参与了绞股蓝内酯介导的葡萄根系结构调控。

IF 10.6

Molecular Horticulture Pub Date : 2024-10-25 DOI: 10.1186/s43897-024-00117-z

Yan Xu, Zhengxin Lv, Muhammad Aamir Manzoor, Linhong Song, Maosen Wang, Lei Wang, Shiping Wang, Caixi Zhang, Songtao Jiu

{"title":"VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture.","authors":"Yan Xu, Zhengxin Lv, Muhammad Aamir Manzoor, Linhong Song, Maosen Wang, Lei Wang, Shiping Wang, Caixi Zhang, Songtao Jiu","doi":"10.1186/s43897-024-00117-z","DOIUrl":"10.1186/s43897-024-00117-z","url":null,"abstract":"The D14 protein, an alpha/beta hydrolase, is a key receptor in the strigolactone (SL) signaling pathway. However, the response of VvD14 to SL signals and its role in grapevine root architecture formation remain unclear. This study demonstrated that VvD14c was highly expressed in grapevine tissues and fruit stages than other VvD14 isoforms. Application of GR24, an SL analog, enhanced the elongation and diameter of adventitious roots but inhibited the elongation and density of lateral roots (LRs) and increased VvD14c expression. Additionally, GR24 is nested within the VvD14c pocket and strongly bound to the VvD14c protein, with an affinity of 5.65 × 10-9 M. Furthermore, VvD14c interacted with grapevine MORE AXILLARY GROWTH 2 (VvMAX2) in a GR24-dependent manner. Overexpression of VvD14c in the d14 mutant and VvMAX2 in the max2 Arabidopsis mutant reversed the increased LR number and density, as well as primary root elongation. Conversely, homologous overexpression of VvD14c and VvMAX2 resulted in reduced LR number and density in grapevines. VvMAX2 directly interacted with LATERAL ORGAN BOUNDARY (VvLOB) and VvLBD19, thereby positively regulating LR density. These findings highlight the role of SLs in regulating grapevine root architecture, potentially via the VvD14c-VvMAX2-VvLOB/VvLBD19 module, providing new insights into the regulation of root growth and development in grapevines.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"4 1","pages":"40"},"PeriodicalIF":10.6,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11515387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovule initiation in crops characterized by multi-ovulate ovaries. 以多卵巢为特征的作物的胚珠萌发。

IF 10.6

Molecular Horticulture Pub Date : 2024-10-18 DOI: 10.1186/s43897-024-00116-0

Yuan-Xin Wang, Xian-Chen Geng, Lu-Han Yang, Ze-Yu Xiong, Yu-Tong Jiang, Jian Pan, Wen-Hui Lin

引用次数: 0

CyDotian: a versatile toolkit for identification of intragenic repeat sequences. CyDotian：用于识别基因内重复序列的多功能工具包。

IF 10.6

Molecular Horticulture Pub Date : 2024-10-09 DOI: 10.1186/s43897-024-00113-3

Huilong Chen, Gang Xu, Weina Ge, Shuyan Feng, Yanli Lin, Changqing Guo, Qianyi Jing, Xuekai Wang, Luiz Gustavo Nussio, Xiyin Wang, Fuyu Yang

引用次数: 0

Functions of membrane proteins in regulating fruit ripening and stress responses of horticultural crops. 膜蛋白在调节园艺作物果实成熟和应激反应中的功能。