Molecular Horticulture最新文献

Cold storage delays peach fruit softening via m⁶A reader PpYTHDFE1 liquid-liquid phase separation-mediated degradation of cell wall-loosening transcript PpEXP3. 低温贮藏通过m6A读取器PpYTHDFE1液-液相分离介导的细胞壁疏松转录物PpEXP3降解延缓桃果果软化。

IF 8.1

Molecular Horticulture Pub Date : 2026-05-09 DOI: 10.1186/s43897-025-00218-3

Hanqing Wang, Hanxiao Bian, Xubin Wu, Kunsong Chen, Donald Grierson, Bo Zhang

{"title":"Cold storage delays peach fruit softening via m6A reader PpYTHDFE1 liquid-liquid phase separation-mediated degradation of cell wall-loosening transcript PpEXP3.","authors":"Hanqing Wang, Hanxiao Bian, Xubin Wu, Kunsong Chen, Donald Grierson, Bo Zhang","doi":"10.1186/s43897-025-00218-3","DOIUrl":"https://doi.org/10.1186/s43897-025-00218-3","url":null,"abstract":"N6-methyladenosine (m6A), the most prevalent epitranscriptomic modification in plant mRNAs, functions as a critical regulatory layer governing environmental response. Refrigeration reduces transcripts of cell wall enzymes to delay softening, however whether m6A participated in this progress remains unclear. Here, we identified a cold-inducible m6A reader PpYTHDFE1 as a regulator that stabilizes peach (Prunus persica L.) fruit firmness. Epitranscriptomic analysis revealed that firmness maintenance correlates with reduced levels of m6A-modified transcripts such as expansin PpEXP3, whose overexpressing accelerates fruit softening. Both in vitro RNA-EMSA and in vivo RIP-qPCR assays demonstrate that PpYTHDFE1 recognizes m6A-modified PpEXP3 transcripts. Transcription inhibition assays further confirm that PpYTHDFE1 accelerates the degradation of PpEXP3 through RNA-protein interaction rather than transcriptional modulation. Mechanistically, PpYTHDFE1 participates in liquid-liquid phase separation as evidenced by fluorescence recovery after photobleaching (FRAP) assays, facilitating PpEXP3 degradation. Furthermore, overexpression of PpYTHDFE1 decreases PpEXP3 mRNA levels, while silencing PpYTHDFE1 leads to PpEXP3 transcript accumulation. A significantly negative correlation was observed between PpYTHDFE1 transcripts and firmness during fruit cold storage. Fruit firmness is increased by 44% in peach and 45% in tomato upon PpYTHDFE1 overexpressing. Our findings provide molecular insights into RNA modification-based strategies to reduce postharvest losses and develop cold-resilient horticultural crops.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergent roles of BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 in regulating the leaf greenness of non-heading Chinese cabbage. BcGUN4.1、BcSG1、BcCHLH和BcTPR4在无头大白菜叶片绿度调控中的趋同作用

IF 8.1

Molecular Horticulture Pub Date : 2026-05-08 DOI: 10.1186/s43897-025-00216-5

Aimei Bai, Li Gong, Xinya Wang, Huanhuan Xu, Zhenzhi Zhu, Changwei Zhang, Tongkun Liu, Xilin Hou, Ying Li

{"title":"Convergent roles of BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 in regulating the leaf greenness of non-heading Chinese cabbage.","authors":"Aimei Bai, Li Gong, Xinya Wang, Huanhuan Xu, Zhenzhi Zhu, Changwei Zhang, Tongkun Liu, Xilin Hou, Ying Li","doi":"10.1186/s43897-025-00216-5","DOIUrl":"10.1186/s43897-025-00216-5","url":null,"abstract":"Non-heading Chinese cabbage (NHCC) extensively cultivated as a leafy vegetable in China, displays diverse leaf coloration, with green being an essential trait. Leaf greenness, determined by chlorophyll and carotenoid contents, is a crucial visual characteristic, and understanding its genetic and molecular mechanisms is essential for breeding. This study identified two highly homologous BcGUN4 genes (BcGUN4.1 and BcGUN4.2) in NHCC. BcGUN4.1 exhibited localization to chloroplasts and cell membranes, positively regulating pigment accumulation. Similarly, BcSG1 and BcCHLH were detected in chloroplasts and cell membranes, co-localizing with BcGUN4.1. Notably, BcGUN4.1 interacted with BcSG1 and BcCHLH in chloroplasts, while BcSG1 functioned as a positive regulator of pigmentation. Moreover, BcGUN4.1, BcSG1, and BcCHLH interacted with BcTPR4, and BcSG1 directly bound to BcCHLH, forming a tetrameric complex in chloroplasts. BcTPR4 contributed to pigment accumulation. Additionally, BcSG1 and BcTPR4 enhanced BcGUN4.1-BcCHLH complex activity. These findings established that BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 cooperatively regulate leaf greenness in NHCC, offering novel insights into the molecular mechanisms underlying this trait.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13154631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA7503 decreases peach (Prunus persica) branch number and angle by inducing pre-miR395a degradation and reducing bioactive BR content. LncRNA7503通过诱导pre-miR395a降解，降低生物活性BR含量，降低桃树枝数和枝角。

IF 8.1

Molecular Horticulture Pub Date : 2026-05-07 DOI: 10.1186/s43897-025-00215-6

Xiaobei Wang, Hefang Xie, Wan Pei, Ruixian Shen, Yajia Zhang, Junjie Zhang, Xiaodong Lian, Haipeng Zhang, Xianbo Zheng, Nan Hou, Lei Wang, Jun Cheng, Wei Wang, Langlang Zhang, Xia Ye, Jidong Li, Jiancan Feng, Bin Tan

{"title":"LncRNA7503 decreases peach (Prunus persica) branch number and angle by inducing pre-miR395a degradation and reducing bioactive BR content.","authors":"Xiaobei Wang, Hefang Xie, Wan Pei, Ruixian Shen, Yajia Zhang, Junjie Zhang, Xiaodong Lian, Haipeng Zhang, Xianbo Zheng, Nan Hou, Lei Wang, Jun Cheng, Wei Wang, Langlang Zhang, Xia Ye, Jidong Li, Jiancan Feng, Bin Tan","doi":"10.1186/s43897-025-00215-6","DOIUrl":"10.1186/s43897-025-00215-6","url":null,"abstract":"Peach (Prunus persica L.) branch number and angle significantly influence orchard management efficiency, fruit yield, and quality. However, few reports exist on genes regulating both traits characteristics. This study utilized miRNA sequencing to identify PpCYP734A14, one of four BR pathway-related genes targeted by differentially expressed miRNAs, as a participant in BR metabolism with elevated expression in pillar-type peach cultivar Zhaoshouhong (ZSH). Transient expression analyses confirmed that miR395a-3p targets PpCYP734A14. The overexpression of pre-miR395a led to significant reduction in PpCYP734A14 mRNA levels and increased bioactive BR content, resulting in enhanced branching and branch angle. Conversely, miR395a-3p silencing produced opposite effects. LncRNA-miRNA-mRNA network analysis revealed that differentially expressed lncRNA7503 reduced mature miR395a-3p levels by promoting the posttranscriptional degradation of pre-miR395a, subsequently increasing PpCYP734A14 expression. These findings implicate PpCYP734A14 in the regulation of both branch number and angle through a lncRNA7503-miR395a-3p mechanism. This pathway presents valuable molecular tools for genetic improvement of tree architecture and facilitates labor-saving cultivation approaches.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13151148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epitranscriptomic profiling of cytosine N4 acetylation (ac⁴C) in Solanum lycopersicum and dynamic changes under heat stress condition. 番茄茄胞嘧啶N4乙酰化（ac4C）的表转录组学分析及热胁迫下的动态变化

IF 8.1

Molecular Horticulture Pub Date : 2026-05-06 DOI: 10.1186/s43897-025-00214-7

Wanhong Zhang, Yubing Jiao, Yanxiao Bu, Lili Shen, Yingwen Wang, Ying Li, Binna Lv, Qi Huang, Yansong Xiao, Tianbo Liu, Jinguang Yang

{"title":"Epitranscriptomic profiling of cytosine N4 acetylation (ac4C) in Solanum lycopersicum and dynamic changes under heat stress condition.","authors":"Wanhong Zhang, Yubing Jiao, Yanxiao Bu, Lili Shen, Yingwen Wang, Ying Li, Binna Lv, Qi Huang, Yansong Xiao, Tianbo Liu, Jinguang Yang","doi":"10.1186/s43897-025-00214-7","DOIUrl":"https://doi.org/10.1186/s43897-025-00214-7","url":null,"abstract":"N4-acetylcytidine (ac4C), which is an evolutionarily conserved RNA modification in eukaryotes, functions as a critical epitranscriptomic regulator that enhances mRNA stability and translation efficiency. In Arabidopsis thaliana and Oryza sativa, ac4C has been characterized as a modulator of transcriptome homeostasis that functions by regulating translational efficiency and maintaining RNA structure; however, the distribution patterns and biological functions of ac4C in other plant species remain largely unexplored. A transcriptome-wide map of the ac4C of Solanum lycopersicum revealed conserved features of plant ac4C modification, with preferential enrichment in mRNA translation initiation and termination regions as well as high conservation of modification motifs and functional categories of target genes across species. Notably, heat stress triggered global ac4C hyperacetylation, and hypermodified genes were enriched in transcripts encoding photosynthesis and thermotolerance-associated regulators. Multiomics integration further revealed that hyperacetylated and upregulated genes were significantly enriched in stress-response pathways, accompanied by elevated transcription levels. Silencing of SLNAT10 resulted in reduced stability of highly acetylated transcripts under heat stress conditions. These findings reveal that RNA acetylation is synchronized with posttranscriptional regulation that promotes transcriptional reprogramming to establish heat tolerance, providing a mechanism for the epitranscriptomic control of environmental adaptation in plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carotenoid aggregates negatively impact chlorophyll levels and disrupt chloroplast development in peaches. 类胡萝卜素聚集体对桃的叶绿素水平产生负面影响，破坏叶绿体发育。

IF 8.1

Molecular Horticulture Pub Date : 2026-04-10 DOI: 10.1186/s43897-025-00213-8

Pengfei Wang, Xulei Zhao, Lipeng Liu, Caizhen Gao, Kaijie Zhu, Jiahui Wang, Yan Han, Xueying Zhang, Haijiang Chen, Li Li, Hongbo Cao

{"title":"Carotenoid aggregates negatively impact chlorophyll levels and disrupt chloroplast development in peaches.","authors":"Pengfei Wang, Xulei Zhao, Lipeng Liu, Caizhen Gao, Kaijie Zhu, Jiahui Wang, Yan Han, Xueying Zhang, Haijiang Chen, Li Li, Hongbo Cao","doi":"10.1186/s43897-025-00213-8","DOIUrl":"10.1186/s43897-025-00213-8","url":null,"abstract":"Peach (Prunus persica L. Batsch) is among the most economically important fruit tree crops. Carotenoids in peach fruit have been intensively studied because of their relationship with fruit color and nutritional value. High carotenoid accumulation is generally a desirable trait in fruits and is associated with their aggregation in plastids. However, the understanding of the functions of carotenoid aggregates is still limited, especially with respect to their effects on chromoplast differentiation. The present study revealed that carotenoid aggregates have significant biological effects on peach. Lycopene and β-carotene aggregates promoted chlorophyll degradation and thylakoid membrane remodeling, leading to the differentiation of chloroplasts into chromoplasts in peach fruit and callus. However, the phytoene and phytofluene did not produce this effect in peach fruit. Interestingly, during lycopene and β-carotene aggregation, the stay-green-like (SGRL) gene was significantly activated, and further confirmed that it was an important factor affecting peach chlorophyll degradation and carotenoid accumulation. Furthermore, molecular dynamics analysis confirmed that lycopene and β-carotene had a greater effect on membrane thickness and rigidity than phytoene. This study contributes to a deeper understanding of peach carotenoid aggregates and their biological effects, confirming the effectiveness of the transgenic peach callus system.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13067727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147646800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-quality genome assembly of Aglaia odorata reveals evolution, terpenes diversity and abundance in Meliaceae. 高质量的香缕草基因组组装揭示了香缕草科植物萜类的进化、多样性和丰度。

IF 8.1

Molecular Horticulture Pub Date : 2026-04-09 DOI: 10.1186/s43897-025-00212-9

Zhiyu Chen, Xingyu Yang, Tianyu Yang, Xin Yin, Danni Yang, Xuefei Yang, Yunqiang Yang, Yongping Yang

{"title":"High-quality genome assembly of Aglaia odorata reveals evolution, terpenes diversity and abundance in Meliaceae.","authors":"Zhiyu Chen, Xingyu Yang, Tianyu Yang, Xin Yin, Danni Yang, Xuefei Yang, Yunqiang Yang, Yongping Yang","doi":"10.1186/s43897-025-00212-9","DOIUrl":"10.1186/s43897-025-00212-9","url":null,"abstract":"Meliaceae species are known for producing diverse metabolites, yet the role of whole-genome duplication (WGD) in shaping this diversity remains unclear. In this study, we analyzed volatile metabolites in Azadirachta indica, Toona ciliata and Aglaia odorata, finding terpenes to be most abundant in A. odorata. A high-quality genome assembly of A. odorata revealed lineage-specific whole-genome triplication (WGT), the first reported in Meliaceae. Comparative genomics indicated extensive chromosomal rearrangements, biased gene retention, gene expression, and long terminal repeat (LTR) insertions during the evolution of subgenomes in Meliaceae, with A. odorata showing subgenome dominance. Despite a reduction in terpene synthase (TPS) gene copies, the specifically highly expression of AodTPS25, AodTPS21, AodTPS24 and AodTPS26 in A. odorata could be crucial for its terpene abundance and diversity through transcriptomics and enzyme assays. Moreover, the ethylene response factor AodERF27 was found to activate AodTPS26, linking transcriptional regulation to terpene diversity. These findings uncovered the genomic and molecular mechanisms in Meliaceae.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissecting G-protein signaling pathways in the fruit pathogen Penicillium expansum: implications for pathogenesis and patulin production. 解剖果实病原菌扩张青霉的g蛋白信号通路：对发病机制和展霉素产生的影响。

IF 8.1

Molecular Horticulture Pub Date : 2026-04-08 DOI: 10.1186/s43897-025-00211-w

Yong Chen, Dongying Xu, Mengyang Xing, Tong Chen, Boqiang Li, Shiping Tian

{"title":"Dissecting G-protein signaling pathways in the fruit pathogen Penicillium expansum: implications for pathogenesis and patulin production.","authors":"Yong Chen, Dongying Xu, Mengyang Xing, Tong Chen, Boqiang Li, Shiping Tian","doi":"10.1186/s43897-025-00211-w","DOIUrl":"10.1186/s43897-025-00211-w","url":null,"abstract":"Penicillium expansum causes blue mold in various fruits and produces patulin, a mycotoxin of significant economic and food safety concern. Fungal infection relies on sensing and adapting to environmental cues via G-protein signaling pathways. Despite its importance, the understanding of how these pathways contribute to the infection process and patulin biosynthesis of P. expansum remains limited. Here, we characterized five G protein subunits (PeGαⅠ, PeGαⅡ, PeGαⅢ, PeGβ, and PeGγ), all highly expressed during hyphal growth and fruit infection. Deletion of these G protein genes leads to various defects in hyphal growth, virulence, and patulin production. Notably, ΔPeGαⅠ, ΔPeGβ, and ΔPeGγ mutants showed severe defects, with minimal disease symptoms in early infection stages and at least a 50% decrease in patulin production. These mutants displayed straight hyphal growth, reduced branching, and significantly decreased vitality. RNA sequencing analysis revealed that this abnormal growth is governed by pathways associated with fungal hyphal polarity. Furthermore, PeGαⅠ and PeGαⅢ are crucial for cAMP-PKA signaling, while PeGβ and PeGγ modulate MAPK signaling, both pathways influencing virulence and patulin production. Together, these findings provide evidence that G proteins regulate virulence and patulin production through the regulation of downstream pathways and their broader effects on fungal hyphal polarity.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147634490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haplotype-resolved genome assembly provides insights into the unique floral scent of Rosa rugosa originated in China. 单倍型解析基因组组装提供了对原产于中国的玫瑰独特花香的见解。

IF 8.1

Molecular Horticulture Pub Date : 2026-04-07 DOI: 10.1186/s43897-025-00210-x

Xi Cheng, Xin Geng, Dan Gao, Hongli Wang, Guoliang Wang, Dongliang Chen, Kang Gao, Tianyi Wang, Chengzhi Jiao, Beibei Jiang, Conglin Huang, Fei Shen

{"title":"Haplotype-resolved genome assembly provides insights into the unique floral scent of Rosa rugosa originated in China.","authors":"Xi Cheng, Xin Geng, Dan Gao, Hongli Wang, Guoliang Wang, Dongliang Chen, Kang Gao, Tianyi Wang, Chengzhi Jiao, Beibei Jiang, Conglin Huang, Fei Shen","doi":"10.1186/s43897-025-00210-x","DOIUrl":"10.1186/s43897-025-00210-x","url":null,"abstract":"Rosa rugosa is an important aromatic plant and produces flowers that are used in medicine, food, plant essential oils, hydrosols, and other aromatic products. 2-phenylethanol (2-PE) is the main effective substance in the volatile organic compounds of rose fragrance, and its synthesis mechanism in R. rugosa needs to be investigated. We herein present a haplotype-resolved genome of R. rugosa cv. Hanxiang (HX). Pathways mediating the synthesis of scent-related metabolites were deciphered in depth. Allelic imbalances reveal the distinct roles of different haplotyping in shaping the key trait including fragrance during the flower development stages. We deciphered the population structure and genetic composition of rose. Selected and mutated genes in groups of separated aromas including but not limited to one rate-limiting enzymes, primary amine oxidases, is responsible for the high level of 2-PE biosynthesis in R. rugosa, which could provide new genetic resources for enhancing aroma in other species.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147628790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MaHSFA2c modulates high temperature-inhibited chlorophyll breakdown in banana fruit by directly inducing the transcription of the E3 ligase gene MaBAH1. MaHSFA2c通过直接诱导E3连接酶基因MaBAH1的转录调节香蕉果实中高温抑制的叶绿素分解。

IF 8.1

Molecular Horticulture Pub Date : 2026-04-03 DOI: 10.1186/s43897-025-00209-4

Wei Wei, Meng-Xue Liu, Jia Si, Qi Luo, Yumei Zhang, Jian-Fei Kuang, Jian-Ye Chen, Wang-Jin Lu, Wei Shan

{"title":"MaHSFA2c modulates high temperature-inhibited chlorophyll breakdown in banana fruit by directly inducing the transcription of the E3 ligase gene MaBAH1.","authors":"Wei Wei, Meng-Xue Liu, Jia Si, Qi Luo, Yumei Zhang, Jian-Fei Kuang, Jian-Ye Chen, Wang-Jin Lu, Wei Shan","doi":"10.1186/s43897-025-00209-4","DOIUrl":"10.1186/s43897-025-00209-4","url":null,"abstract":"Bananas (Musa acuminata, AAA group) ripening at temperatures higher than 24 °C prevent yellow skin development, resulting in green ripening, and significantly affecting the commercial potential of the fruit. Our recent study discovered that high temperature (HT) triggers the E3 ubiquitin ligase MaBAH1 to degrade MaMYB60, thereby suppressing MaMYB60's transactivation of chlorophyll catabolic genes. However, the mechanism by which HT stimulates MaBAH1 expression remains unknown. In this study, a heat shock transcription factor, termed MaHSFA2c, was identified as an upstream regulator of MaBAH1. MaHSFA2c gene expression and protein levels were up-regulated in green-ripened fruit at 30 °C compared to yellow-ripened fruit at 20 °C. MaHSFA2c directly activated the transcription of MaBAH1. Importantly, silencing MaHSFA2c in banana fruit suppressed MaBAH1 expression, reducing MaBAH1-mediated MaMYB60 degradation. This promoted MaMYB60's activation of chlorophyll degradation, thereby weakening green-ripening at HT. In the context of banana fruit, we have demonstrated the existence of a dynamic regulatory module consisting of MaHSFA2c and MaBAH1 that manages chlorophyll catabolism inhibition under HT. These findings enhance our understanding of the transcriptional regulation involved in the loss of fruit quality due to temperature stress and are expected to aid in developing strategies to manage banana green-ripening.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine-mapping reveals BcNAC153 act as a negative regulator controlling high temperature tolerance in Brassica rapa. 精细图谱显示，BcNAC153在油菜耐高温性中起负调控作用。

IF 8.1

Molecular Horticulture Pub Date : 2026-04-02 DOI: 10.1186/s43897-025-00208-5

Gaofeng Liu, Entong Li, Qian Hu, Yaliang Xu, Changwei Zhang, Ying Li, Zixin Zhang, Xilin Hou

{"title":"Fine-mapping reveals BcNAC153 act as a negative regulator controlling high temperature tolerance in Brassica rapa.","authors":"Gaofeng Liu, Entong Li, Qian Hu, Yaliang Xu, Changwei Zhang, Ying Li, Zixin Zhang, Xilin Hou","doi":"10.1186/s43897-025-00208-5","DOIUrl":"10.1186/s43897-025-00208-5","url":null,"abstract":"High temperature stress severely reduces yield and quality in cool-climate adapted NHCC vegetable crops. It is crucial to identify high temperature response regulating genes and analyze the molecular mechanisms of NHCC in order to develop heat-resistant varieties. Here, a whole genome resequencing was conducted on NHCC001, NHCC002, and 312 F2 populations for QTLs mapping. A high-density bin-map containing 3608 bin markers was constructed covering 1156.88 cM, with an average adjacent interval of 0.32 cM. A major QTL for high temperature stress tolerance was identified in A01 chromosome, explaining 6.101% of phenotype variation. And BcNAC153, a NAC transcription factor, was captured and validated as a critical negative regulator for high temperature tolerance. The promoter activity of BcNAC153 was found to be regulated by high temperature, and BcMYB44 can inhibit the transcription of BcNAC153 by binding to the promoter of BcNAC153. In addition, this study reveals that BcCRK1 facilitates the ubiquitin-mediated degradation of BcNAC153, which affects the expression of downstream genes related to chlorophyll degradation, PCD, and ROS accumulation. Overall, this study sheds light on the molecular mechanisms underlying the response of NHCC to high temperature stress, and provides valuable insights for developing heat-resistant vegetable varieties.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147595313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0