Molecular Horticulture最新文献

Regulation of transcriptional homeostasis by DNA methylation upon genome duplication in pak choi.

IF 10.6

Molecular Horticulture Pub Date : 2025-04-05 DOI: 10.1186/s43897-025-00145-3

Min Ma, Yuanda Wang, Zhenfei Sun, Ranze Zhao, Honghua Li, Xiaoxuan Li, Hongfang Zhu, Xuedong Yang, Changwei Zhang, Yuda Fang

{"title":"Regulation of transcriptional homeostasis by DNA methylation upon genome duplication in pak choi.","authors":"Min Ma, Yuanda Wang, Zhenfei Sun, Ranze Zhao, Honghua Li, Xiaoxuan Li, Hongfang Zhu, Xuedong Yang, Changwei Zhang, Yuda Fang","doi":"10.1186/s43897-025-00145-3","DOIUrl":"10.1186/s43897-025-00145-3","url":null,"abstract":"Polyploidy occurs frequently in plants and is an important force in plant evolution and crop breeding. New polyploids face various challenges due to genome duplication and subsequent changes in epigenetic modifications, nucleus/cell size and gene expression. How polyploids produce evolutionary novelty remains to be understood. In this study, a transcriptome comparison between 21-day-old diploid and autotetraploid pak choi seedlings revealed that there are few differentially expressed genes (DEGs), with a greater proportion of DEGs downregulated in response to genome duplication. Genome-wide DNA methylation analysis indicated that the level of DNA methylation is obviously increased, especially in transposable elements (TEs) and 1 kb flanking regions, upon genome doubling. The differentially methylated regions between diploid and autotetraploid pak choi were related to 12,857 differentially hypermethylated genes and 8,451 hypomethylated genes, and the DEGs were negatively correlated with the differential methylation in the regions across the DEGs. Notably, TE methylation increases significantly in regions flanking neighboring non-DEGs rather than those flanking DEGs. These results shed light on the role of DNA methylation in the transcriptional regulation of genes in polyploids and the mechanism of coping with \"genome shock\" due to genome doubling in cruciferous plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"22"},"PeriodicalIF":10.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AcNAC10, regulated by AcTGA07, enhances kiwifruit resistance to Pseudomonas syringae pv. actinidiae via inhibiting jasmonic acid pathway.

IF 10.6

Molecular Horticulture Pub Date : 2025-04-04 DOI: 10.1186/s43897-024-00143-x

Chao Zhao, Wei Liu, Chenxiao Yao, Yali Zhang, Xiaofei Du, Chao Ma, Rui Li, Hua Wang, Lili Huang

{"title":"AcNAC10, regulated by AcTGA07, enhances kiwifruit resistance to Pseudomonas syringae pv. actinidiae via inhibiting jasmonic acid pathway.","authors":"Chao Zhao, Wei Liu, Chenxiao Yao, Yali Zhang, Xiaofei Du, Chao Ma, Rui Li, Hua Wang, Lili Huang","doi":"10.1186/s43897-024-00143-x","DOIUrl":"10.1186/s43897-024-00143-x","url":null,"abstract":"Kiwifruit bacterial canker is a devastating disease caused by Pseudomonas syringae pv. actinidiae (Psa). NAC transcription factors play a significant role in host immunity. However, the potential molecular mechanism of resistance to semi-biotrophic Psa mediated by NAC transcription factors in kiwifruit remains unclear. In this study, we identified a typical NAC transcription factor, AcNAC10, which is involved in the jasmonic acid (JA) pathway and is highly expressed in resistant variety RH12 responsing to Psa. By overexpression and silencing of AcNAC10 in kiwifruit, it plays a positive role in enhancing kiwifruit resistance. Likewise, heterologous expression of AcNAC10 in transgenic Arabidopsis and tomato enhanced resistance to P. syringae. By directly binding to the promoter of AcLOX3, AcNAC10 inhibited its expression as a transcriptional suppressor. Using a yeast one-hybrid screening library, electrophoretic mobility shift assay (EMSA), and dual-luciferase reporter assays, it showed that AcTGA07 can activate the expression of AcNAC10. Moreover, we demonstrated that AcTGA07 decreased JA accumulation independently of the AcNAC10-AcLOX3 pathway. Our study elucidated the transcriptional cascade regulatory network of AcTGA07-AcNAC10-AcLOX3, which enhanced the disease resistance of kiwifruit to Psa by inhibiting JA synthesis.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"21"},"PeriodicalIF":10.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive characterization of volatile terpenoids and terpene synthases in Lanxangia tsaoko.

IF 10.6

Molecular Horticulture Pub Date : 2025-04-03 DOI: 10.1186/s43897-024-00140-0

Shanshan Chen, Mofan Zhang, Shuo Ding, Zhichao Xu, Sifan Wang, Xiangxiao Meng, Shilin Chen, Ranran Gao, Wei Sun

{"title":"Comprehensive characterization of volatile terpenoids and terpene synthases in Lanxangia tsaoko.","authors":"Shanshan Chen, Mofan Zhang, Shuo Ding, Zhichao Xu, Sifan Wang, Xiangxiao Meng, Shilin Chen, Ranran Gao, Wei Sun","doi":"10.1186/s43897-024-00140-0","DOIUrl":"10.1186/s43897-024-00140-0","url":null,"abstract":"Lanxangia tsaoko is widely utilized in human cuisine as a popular flavoring agent due to its distinctive aroma. It also has a long history of use in traditional Chinese medicine. The edible and medicinal properties of L. tsaoko are primarily attributed to its diverse array of volatile metabolites. Previous research has mainly focused on classifying the constituents and their pharmacological activities in L. tsaoko, leaving gaps in comprehensive identification and elucidation of the biosynthetic mechanisms of these metabolites. In this study, we employed a multi-omics approach and functional characterization to investigate the biosynthesis of volatile terpenoids in L. tsaoko. The results demonstrated that terpenoids constituted the highest proportion of volatile compounds in L. tsaoko. Additionally, 42 terpene synthase (TPS) coding genes were identified through genome-wide analysis. Functional characterization revealed that eight LtTPSs effectively catalyzed geranyl pyrophosphate to produce monoterpenoids, while four LtTPSs converted farnesyl pyrophosphate to generate sesquiterpenoids. Genome-wide and single-gene duplication events contributed to functional diversification among LtTPSs with high identity, promoting the diversity of terpenoids. These findings provide a foundation for understanding the biosynthesis of volatile terpenoids in L. tsaoko, enhance the current knowledge of TPS, and contribute to the broader understanding of the biochemical diversity of terpenoids in plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"20"},"PeriodicalIF":10.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143774445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatic symphony of fleshy fruits: functions, biosynthesis and metabolic engineering of bioactive compounds.

IF 10.6

Molecular Horticulture Pub Date : 2025-04-02 DOI: 10.1186/s43897-024-00142-y

Huimin Hu, Nirakar Pradhan, Jianbo Xiao, Rui Xia, Pan Liao

{"title":"Chromatic symphony of fleshy fruits: functions, biosynthesis and metabolic engineering of bioactive compounds.","authors":"Huimin Hu, Nirakar Pradhan, Jianbo Xiao, Rui Xia, Pan Liao","doi":"10.1186/s43897-024-00142-y","DOIUrl":"10.1186/s43897-024-00142-y","url":null,"abstract":"Fleshy fruits are popular among consumers due to their significant nutritional value, which includes essential bioactive compounds such as pigments, vitamins, and minerals. Notably, plant-derived pigments are generally considered safe and reliable, helping to protect humans against various inflammatory diseases. Although the phytochemical diversity and their biological activities have been extensively reviewed and summarized, the status of bioactive nutrients in fleshy fruits, particularly with a focusing on different colors, has received less attention. Therefore, this review introduces five common types of fleshy fruits based on coloration and summarizes their major bioactive compounds. It also provides the latest advancements on the function, biosynthesis, and metabolic engineering of plant-derived pigments. In this review, we emphasize that promoting the consumption of a diverse array of colorful fruits can contribute to a balanced diet; however, optimal intake levels still require further clinical validation. This review may serve as a useful guide for decisions that enhance the understanding of natural pigments and accelerate their application in agriculture and medicine.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"19"},"PeriodicalIF":10.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta).

IF 10.6

Molecular Horticulture Pub Date : 2025-04-01 DOI: 10.1186/s43897-024-00139-7

Yukuo Li, Zhe Song, Xu Zhan, Xiaohan Li, Lingshuai Ye, Miaomiao Lin, Ran Wang, Hailei Huang, Jian Guo, Leiming Sun, Hong Gu, Jinyong Chen, Jinbao Fang, Xiujuan Qi

{"title":"Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta).","authors":"Yukuo Li, Zhe Song, Xu Zhan, Xiaohan Li, Lingshuai Ye, Miaomiao Lin, Ran Wang, Hailei Huang, Jian Guo, Leiming Sun, Hong Gu, Jinyong Chen, Jinbao Fang, Xiujuan Qi","doi":"10.1186/s43897-024-00139-7","DOIUrl":"10.1186/s43897-024-00139-7","url":null,"abstract":"Actinidia arguta is a newly emerged, commercially cultivated Actinidia species. A. arguta has a beautiful appearance and is rich in anthocyanin, and is thus highly welcomed by consumers. However, the mechanism of anthocyanin regulation in A. arguta remains unclear. In this study, we assembled the nearly complete genome of the first red A. arguta cultivar, 'Tianyuanhong', with an N50 of 21 Mb. Comparative genome analysis revealed a role of the expansion/contraction of gene families in the species-specific trait formation of A. arguta. Through verification of transient overexpression and stable transformation, RNA-seq analysis revealed a key bHLH transcription factor, AaBEE1, which negatively regulates anthocyanin biosynthesis. DAP-seq analysis combined with Y1H, EMSA, Chip-qPCR and LUC suggested that AaBEE1 binds to the G-box of the AaLDOX promoter and suppresses its expression. Overall, we assembled the genome of A. arguta and clarified its AaBEE1-AaLDOX module-mediated molecular mechanism of anthocyanin regulation.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"18"},"PeriodicalIF":10.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Super pan-genome reveals extensive genomic variations associated with phenotypic divergence in Actinidia.

IF 10.6

Molecular Horticulture Pub Date : 2025-03-19 DOI: 10.1186/s43897-025-00154-2

Xiaofen Yu, Minghao Qu, Pan Wu, Miao Zhou, Enhui Lai, Huan Liu, Sumin Guo, Shan Li, Xiaohong Yao, Lei Gao

引用次数: 0

Two TAL effectors of Xanthomonas citri promote pustule formation by directly repressing the expression of GRAS transcription factor in citrus.

IF 10.6

Molecular Horticulture Pub Date : 2025-03-14 DOI: 10.1186/s43897-024-00131-1

Yichao Yan, Xiaomei Tang, Zhongfeng Zhu, Ke Yin, Yikun Zhang, Zhengyin Xu, Qiang Xu, Lifang Zou, Gongyou Chen

{"title":"Two TAL effectors of Xanthomonas citri promote pustule formation by directly repressing the expression of GRAS transcription factor in citrus.","authors":"Yichao Yan, Xiaomei Tang, Zhongfeng Zhu, Ke Yin, Yikun Zhang, Zhengyin Xu, Qiang Xu, Lifang Zou, Gongyou Chen","doi":"10.1186/s43897-024-00131-1","DOIUrl":"10.1186/s43897-024-00131-1","url":null,"abstract":"Citrus bacterial canker (CBC), caused by Xanthomonas citri subsp. citri (Xcc), poses a significant threat to the citrus industry. Xcc employs the transcription activator-like effector (TALE) PthA4 to target the major susceptibility (S) gene CsLOB1 in citrus, promoting host susceptibility to bacterial canker. However, the contribution of other Xcc TALEs, aside from PthA4, to virulence remains underexplored. In this study, we characterized two PthA1 variants, designated PthA5 and PthA6, which facilitate Xcc infection in susceptible citrus species by promoting the formation of hypertrophy and hyperplasia symptoms. Both PthA5 and PthA6 bind directly to effector-binding elements (EBEs) in the promoter of CsGRAS9, suppressing its expression. CsGRAS9 negatively regulates Xcc growth in citrus and contributes to CBC resistance. Notably, natural variations in the EBEs of the FhGRAS9 promoter, a homolog of CsGRAS9 in Hong Kong kumquat, prevent Xcc from affecting FhGRAS9 expression. Using the PTG/Cas9 system, we generated proCsGRAS9-edited sweet orange lines #18-2 and #23, which contain 86-bp and 62-bp deletions in the EBE regions of the CsGRAS9 promoter. These mutant lines showed enhanced CsGRAS9 expression and increased resistance to CBC during Xcc infection. Several GA-related genes and CsTAC1, regulated by CsGRAS9, were also identified. This is the first report that TALEs act as repressors of a resistance gene to confer host susceptibility.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"30"},"PeriodicalIF":10.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate change affects the suitability of Chinese cherry (Prunus pseudocerasus Lindl.) in China.

IF 10.6

Molecular Horticulture Pub Date : 2025-03-06 DOI: 10.1186/s43897-024-00136-w

Zhengxin Lv, Songtao Jiu, Li Wang, Yan Xu, Jiyuan Wang, Xunju Liu, Jieming Xu, Yuxuan Wang, Muhammad Salman Haider, Ruie Liu, Caixi Zhang

{"title":"Climate change affects the suitability of Chinese cherry (Prunus pseudocerasus Lindl.) in China.","authors":"Zhengxin Lv, Songtao Jiu, Li Wang, Yan Xu, Jiyuan Wang, Xunju Liu, Jieming Xu, Yuxuan Wang, Muhammad Salman Haider, Ruie Liu, Caixi Zhang","doi":"10.1186/s43897-024-00136-w","DOIUrl":"10.1186/s43897-024-00136-w","url":null,"abstract":"The rapid development of Prunus pseudocerasus related industry has increasingly contributed to rural vitalization in China. This study employed a biomod2 ensemble model, utilizing environmental and species occurrence data from 151 P. pseudocerasus germplasm wild/local samples, to predict potential geographical distribution, suitability changes, climate dependence, and ecological niche dynamics. The optimized maximum entropy (MaxEnt) model yielded the most accurate predictions. The climate variables with the greatest impact on suitability were precipitation of warmest quarter and mean diurnal temperature range. The total potential suitable area for P. pseudocerasus was approximately 2.78 × 106 km2, increasing with CO2 concentration. The highly suitable area was primarily concentrated in basin terrains, plateaus, and plains of Sichuan Province. The current centroid in Lichuan exhibited gradual latitudinal and longitudinal movement. The predicted (2090s) ecological niche trends of P. pseudocerasus varied under different pathways and periods, with higher CO2 concentration associated with lower niche overlap. The CO2 emission concentration in the SSP246 scenario emerged as the most suitable climate model. Climate change is driving both the expansion of geographical distribution and the contraction of overlapping geographical distribution areas of P. pseudocerasus. These findings provide a theoretical basis for wild resource conservation, site selection for production, and introduction of allopatry for P. pseudocerasus.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"26"},"PeriodicalIF":10.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel mode of WRKY1 regulating PR1-mediated immune balance to defend against powdery mildew in apple.

IF 10.6

Molecular Horticulture Pub Date : 2025-03-05 DOI: 10.1186/s43897-024-00141-z

Liming Lan, Lifang Cao, Lulu Zhang, Weihong Fu, Changguo Luo, Chao Wu, Xianqi Zeng, Shenchun Qu, Xinyi Yu, Wenyi Deng, Xu Xu, Binhua Cai, Sanhong Wang

{"title":"A novel mode of WRKY1 regulating PR1-mediated immune balance to defend against powdery mildew in apple.","authors":"Liming Lan, Lifang Cao, Lulu Zhang, Weihong Fu, Changguo Luo, Chao Wu, Xianqi Zeng, Shenchun Qu, Xinyi Yu, Wenyi Deng, Xu Xu, Binhua Cai, Sanhong Wang","doi":"10.1186/s43897-024-00141-z","DOIUrl":"10.1186/s43897-024-00141-z","url":null,"abstract":"Powdery mildew (PM), caused by the biotrophic fungus Podospharea leucotricha, poses a significant threat to apple production. Salicylic acid (SA) signaling plays a crucial role in enhancing resistance to biotrophic pathogens. While PR1, a defense protein induced by SA, is essential for plant immunity, its excessive accumulation can be detrimental. However, the mechanism of PR1-mediated immune balance remains unclear. This study identified a key transcription factor, WRKY1, which enhances the SA accumulation by modulating the SA biosynthesis gene EPS1, while simultaneously regulating the WRKY40-NPR3g module to prevent sustained PR1 expression caused by continuous SA accumulation. Specifically, the transcription factor WRKY40 upregulates NPR3g expression, and NPR3g interacts with NPR1 in an SA-dependent manner. Then, two TGA2c variants that interact with NPR1 to activate PR1 expression were identified: canonical TGA2c-1 and alternative splicing of TGA2c-2 with an exon deletion. SA does not influence the NPR1-TGA2c-1 interaction but is essential for the NPR1-TGA2c-2 interaction. Notably, NPR3g reduces PR1 levels by selectively disrupting the NPR1-TGA2c-2 complex through competition for the BTB-POZ domain of NPR1. In conclusion, this study identifies a novel mechanism by which WRKY1 modulates PR1-mediated immune balance to defend against PM.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"17"},"PeriodicalIF":10.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA methylation dynamics in male germline development in Brassica Rapa.

IF 10.6

Molecular Horticulture Pub Date : 2025-03-04 DOI: 10.1186/s43897-024-00137-9

Jun Zhang, Di Wu, Yating Zhang, Xiaoqi Feng, Hongbo Gao

{"title":"DNA methylation dynamics in male germline development in Brassica Rapa.","authors":"Jun Zhang, Di Wu, Yating Zhang, Xiaoqi Feng, Hongbo Gao","doi":"10.1186/s43897-024-00137-9","DOIUrl":"10.1186/s43897-024-00137-9","url":null,"abstract":"Dynamic DNA methylation represses transposable elements (TEs) and regulates gene activity, playing a pivotal role in plant development. Although substantial progress has been made in understanding DNA methylation reprogramming during germline development in Arabidopsis thaliana, whether similar mechanisms exist in other dicot plants remains unclear. Here, we analyzed DNA methylation levels in meiocytes, microspores, and pollens of Brassica Rapa using whole-genome bisulfite sequencing (WGBS). Global DNA methylation analysis revealed similar CHH methylation reprogramming compared to Arabidopsis, while distinct patterns were observed in the dynamics of global CG and CHG methylation in B. rapa. Differentially methylated region (DMR) analysis identified specifically methylated loci in the male sex cells of B. Rapa with a stronger tendency to target genes, similar to observations in Arabidopsis. Additionally, we found that the activity and genomic targeting preference of the small RNA-directed DNA methylation (RdDM) were altered during B. Rapa male germline development. A subset of long terminal repeat (LTR) TEs were activated, possibly due to the dynamic regulation of DNA methylation during male sexual development in B. Rapa. These findings provided new insights into the evolution of epigenetic reprogramming mechanisms in plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"16"},"PeriodicalIF":10.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11877836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0