Molecular Horticulture最新文献

FVRS: a cross-species multi-omics database for fruit and vegetable ripening and senescence. FVRS：水果和蔬菜成熟和衰老的跨物种多组学数据库。

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Molecular Horticulture Pub Date : 2026-10-20 DOI: 10.1186/s43897-026-00284-1

Lili Ma, Yunxiang Wang, Yiting Ren, Caie Wu, Yuanye Jiang, Jiejie Tao, Xuelian He, Xinyi Feng, Tianyu Li, Xu Liu, Jingjing Jin, Jinhua Zuo, Yanyan Zheng

引用次数: 0

Machine learning-guided leaf margin classification and discovery that BcPHB promoter selection rewires a BcPHB-BcLMI1 module to shape leaf margins in Brassica campestris. 机器学习引导的叶缘分类和发现BcPHB启动子选择重新连接BcPHB- bclmi1模块来塑造油菜叶缘。

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Molecular Horticulture Pub Date : 2026-10-13 DOI: 10.1186/s43897-026-00251-w

Jingwen Chen, Airong Li, Tianqi Wu, Zhenyu Huang, Xiyao Li, Shan Xu, Yujie Wu, Yuan Tian, Weiwei Chen, Xinhua Quan, Xiangtan Yao, Sue Lin, Xuping Feng, Li Huang

{"title":"Machine learning-guided leaf margin classification and discovery that BcPHB promoter selection rewires a BcPHB-BcLMI1 module to shape leaf margins in Brassica campestris.","authors":"Jingwen Chen, Airong Li, Tianqi Wu, Zhenyu Huang, Xiyao Li, Shan Xu, Yujie Wu, Yuan Tian, Weiwei Chen, Xinhua Quan, Xiangtan Yao, Sue Lin, Xuping Feng, Li Huang","doi":"10.1186/s43897-026-00251-w","DOIUrl":"10.1186/s43897-026-00251-w","url":null,"abstract":"The morphological diversification of leaf margins reflects the evolutionary reshaping of conserved developmental programs. However, fully deciphering the underlying molecular basis remains challenging due to difficulties in phenotype quantification and the incomplete characterization of complex regulatory networks. In this study, we established a computer-vision framework to decode leaf lobe trait and identified the HD-ZIP III factor PHABULOSA (BcPHB), combining genome-wide association studies (GWAS) and selective sweep analysis in Brassica campestris. We found that promoter variation defines two major haplotypes, BcPHBHapI and BcPHBHapII, driving divergent expression levels associated with entire and lobed leaves. Functionally, BcPHB knockdown lines (BcPHBami) exhibited lobed margins, whereas complementation of lobed accessions with the BcPHBHapI promoter partially restored the entire-leaf phenotype. Similarly, overexpression of LATE MERISTEM IDENTITY 1 (BcLMI1), a conserved regulator of leaf lobation, phenocopied the BcPHBami lines. Mechanistically, BcPHB and BcLMI1 form a reciprocal negative feedback loop that stabilizes margin patterning. Furthermore, domestication-driven selection of BcPHBHapI facilitated the transition from ancestral lobed leaves to cultivated entire forms. This study reveals a pivotal developmental module and illustrates how regulatory evolution drives morphological diversification in crops.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AcWRKY75 and AcMYB108 mediated ABA-GA antagonism in the regulation of pineapple internal browning. AcWRKY75和AcMYB108介导ABA-GA拮抗调控菠萝内部褐变。

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Molecular Horticulture Pub Date : 2026-10-11 DOI: 10.1186/s43897-026-00247-6

Qian Li, Guang Wang, Shijiang Zhu

{"title":"AcWRKY75 and AcMYB108 mediated ABA-GA antagonism in the regulation of pineapple internal browning.","authors":"Qian Li, Guang Wang, Shijiang Zhu","doi":"10.1186/s43897-026-00247-6","DOIUrl":"https://doi.org/10.1186/s43897-026-00247-6","url":null,"abstract":"Gibberellic acid (GA) and abscisic acid (ABA) antagonistically regulate various biological processes, but whether they antagonistically regulate tissue browning remains unclear. Here, we showed that ABA reduced pineapple internal browning (IB) by inhibiting phenolic biosynthesis via downregulating AcWRKY75 and AcMYB108, while GA3 had the opposite effects. Biochemical evidences showed AcWRKY75 and AcMYB108 directly bound to and activated the phenolic biosynthetic gene Ac4CL5. Interestingly, AcMYB108 directly bound to AcWRKY75 promoter and activated its transcription, indicating that AcMYB108 functions upstream of AcWRKY75. Arabidopsis overexpressing AcWRKY75, AcMYB108 or Ac4CL5 displayed promoted phenolic biosynthesis. AcWRKY75 transient overexpression in pineapple reduced, while its transient silencing enhanced IB and phenolic accumulation. Transcriptomic profiling of pineapples transiently overexpressing and silencing AcWRKY75 revealed that plant hormone signal transduction and phenylpropanoid biosynthesis were among the most significantly enriched KEGG pathways, suggesting the role of AcWRKY75 in regulating phenolic biosynthesis involves plant hormones. ABA suppressed and GA3 enhanced enzyme activity of GUS transiently expressed in tobacco driven by promoter of AcWRKY75, AcMYB108 or Ac4CL5. This work suggests that WRKY75 and MYB108 are involved in antagonism between ABA and GA in regulating IB, and may shed novel insight into the role of ABA and GA in regulating plant tissue browning.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13654230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative multi-omics analyses reveal transcriptional, metabolic, and hormonal regulation of vernalization-induced flowering in Wucai (Brassica rapa). 综合多组学分析揭示了春化诱导武菜开花的转录、代谢和激素调控。

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Molecular Horticulture Pub Date : 2026-10-10 DOI: 10.1186/s43897-026-00246-7

Yunfei Tian, Danni Yang, Na Liao, Kang Wang, Yuxin Yang, Xinghao Zhou, Ting Li, Wenjie Wang, Xiaoyan Tang, Chenggang Wang, Lingyun Yuan, Guohu Chen

{"title":"Integrative multi-omics analyses reveal transcriptional, metabolic, and hormonal regulation of vernalization-induced flowering in Wucai (Brassica rapa).","authors":"Yunfei Tian, Danni Yang, Na Liao, Kang Wang, Yuxin Yang, Xinghao Zhou, Ting Li, Wenjie Wang, Xiaoyan Tang, Chenggang Wang, Lingyun Yuan, Guohu Chen","doi":"10.1186/s43897-026-00246-7","DOIUrl":"10.1186/s43897-026-00246-7","url":null,"abstract":"Vernalization, a low-temperature-induced transition from vegetative to reproductive growth, is essential for flowering in many Brassica crops, including Wucai (Brassica rapa ssp. chinensis var. rosularis). To elucidate the molecular basis of vernalization in Wucai, we performed an integrative multi-omics analysis of shoot apices from cold-treated plants. Twenty-eight vernalization pathway-related genes, including homologs of FLC, FLM, VIN3, VRN, SOC1, SVP, and VIL2, exhibited conserved cold-responsive expression patterns. Transcription factors from the MYB, bHLH, bZIP, and NAC families showed dynamic regulation, implicating key members such as MYB57, bHLH3, and ABF4 in flowering control and hormonal signaling. Metabolomic profiling revealed significant enrichment of carbohydrate and amino acid metabolism, with increased levels of sucrose, glucose, galactose, glycine, serine, proline, and arginine, suggesting enhanced mobilization of energy and nitrogen during vernalization. Proline accumulation, together with upregulation of P5CS, indicates a potential role in floral transition. Hormonal analysis showed elevated auxin, kinetin, jasmonic acids, and brassinosteroids, with cold-induced BcDET2 expression correlating with increased brassinosteroid levels and early flowering. Exogenous brassinolide further promoted flowering by activating FT and SOC1 while repressing FLC. These findings provide a comprehensive molecular framework for vernalization-regulated flowering in Wucai.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13653411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The glutathione S-transferase gene LaGSTU6 promotes somatic embryogenesis in a recalcitrant lily genotype by regulating scales redox homeostasis. 谷胱甘肽s -转移酶基因LaGSTU6通过调节鳞片氧化还原稳态促进顽固性百合基因型的体细胞胚胎发生。

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Molecular Horticulture Pub Date : 2026-10-09 DOI: 10.1186/s43897-026-00244-9

Yue Ma, Hongyu Li, Hongmei Sun

{"title":"The glutathione S-transferase gene LaGSTU6 promotes somatic embryogenesis in a recalcitrant lily genotype by regulating scales redox homeostasis.","authors":"Yue Ma, Hongyu Li, Hongmei Sun","doi":"10.1186/s43897-026-00244-9","DOIUrl":"10.1186/s43897-026-00244-9","url":null,"abstract":"Genotypic recalcitrance limits efficient somatic embryogenesis (SE). In this study, an SE induction system for the recalcitrant species Lilium amabile Palib. was established by modulating redox homeostasis. The first SE in this species was achieved by supplementing MS medium containing 1.0 mg·L-1 picloram (PIC) with 150.0 mg·L-1 glutathione (GSH), which increased SE induction rate from 0 to 33.3%. Physiological analysis revealed that GSH treatment upregulated endogenous GSH and key antioxidant enzymes, glutathione peroxidase (GPX) and glutathione S-transferase (GST). Specifically, their activities were elevated 1.9- and 9.8-fold higher, respectively, than those of control. This synergistic action scavenged hydrogen peroxide (H₂O₂), thereby alleviating oxidative stress. Furthermore, LaGSTU6 was identified as a core regulator of SE. In the SE-efficient lily Lilium martagon var. pilosiusculum Freyn, transient silencing of LaGSTU6 delayed embryogenesis initiation, caused severe browning, induced H₂O₂ accumulation, and reduced the antioxidant capacity, yielding a significant decrease in both the SE induction rate and efficiency. These findings preliminarily confirm that LaGSTU6 is a critical regulator of SE competence in lilies. Thus, we overcame genotypic recalcitrance and established the first SE induction system in Lilium amabile Palib. Our findings provide insights into the study of totipotency and inform strategies for regenerating recalcitrant germplasms.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HubZIP12-mediated transcriptional activation of betalain biosynthetic genes enhances pigmentation in pitaya (Hylocereus). hubzip12介导的β素生物合成基因转录激活增强了火龙果（Hylocereus）的色素沉着。

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Molecular Horticulture Pub Date : 2026-10-08 DOI: 10.1186/s43897-026-00243-w

Irfan Ali Sabir, Xinglong Hu, Jiayi Chen, Yonghua Qin

引用次数: 0

From parasite to bridge: redefining Cuscuta in plant communication networks. 从寄生虫到桥梁：重新定义植物通讯网络中的Cuscuta。

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Molecular Horticulture Pub Date : 2026-09-11 DOI: 10.1186/s43897-026-00275-2

Seung Hee Eom, Tae Kyung Hyun

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Light-responsive PyNAC90 orchestrates anthocyanin biosynthesis through combinatorial transcriptional activation and COP1-mediated degradation in pear. 光响应PyNAC90通过组合转录激活和cop1介导的降解协调梨花青素的生物合成。

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Molecular Horticulture Pub Date : 2026-09-10 DOI: 10.1186/s43897-026-00254-7

Chenhui Han, Zhaolong Xue, Guosong Chen, Yanfei Shan, Chenjie Yao, Yongqi Zhao, Bo Li, Yule Miao, Jiahao Zeng, Tianyu Wang, Guangyan Yang, Jun Wu

{"title":"Light-responsive PyNAC90 orchestrates anthocyanin biosynthesis through combinatorial transcriptional activation and COP1-mediated degradation in pear.","authors":"Chenhui Han, Zhaolong Xue, Guosong Chen, Yanfei Shan, Chenjie Yao, Yongqi Zhao, Bo Li, Yule Miao, Jiahao Zeng, Tianyu Wang, Guangyan Yang, Jun Wu","doi":"10.1186/s43897-026-00254-7","DOIUrl":"10.1186/s43897-026-00254-7","url":null,"abstract":"Anthocyanins confer diverse biological activities and health benefits, and represent a key quality trait in fruit. In pear, red skin coloration arises from anthocyanin accumulation, a process strongly induced by light. Here, transcriptomic analyses identified PyNAC90 as a candidate regulator promoting anthocyanin biosynthesis. The expression level of PyNAC90 gradually increased with extended bag removal. Stable transformation in pear callus and transient overexpression in pear skin showed that PyNAC90 overexpression significantly enhanced anthocyanin accumulation, whereas PyNAC90 silencing significantly suppressed anthocyanin biosynthesis. Dual-luciferase reporter assays demonstrated that PyHY5 directly induced PyNAC90, while PyNAC90 directly activated PyMYB10 and indirectly activated the downstream structural genes PyDFR and PyANS. Furthermore, co-expression of PyNAC90, PyMYB10, and PybHLH3 synergistically activated the downstream structural genes PyDFR and PyANS. Moreover, Y2H, LCI, and BiFC assays confirmed that PyNAC90 physically interacted with PyMYB10, strengthening cooperative transcriptional regulation of anthocyanin biosynthesis. The E3 ubiquitin ligase PyCOP1 also interacted with PyNAC90 both in vivo and in vitro, mediating its degradation, thereby reducing anthocyanin accumulation. Collectively, our results elucidate a molecular mechanism by which light-responsive NAC transcription factors regulate anthocyanin biosynthesis and expand the regulatory network underlying light-induced pigmentation.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13560111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148921075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A Prunus-specific small peptide suppresses anthocyanin accumulation by targeting HYH-BBX signaling in peach. 一种桃树特异性小肽通过靶向HYH-BBX信号抑制桃树花青素积累。

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Molecular Horticulture Pub Date : 2026-09-09 DOI: 10.1186/s43897-026-00274-3

Lei Zhao, Liao Liao, Weihan Zhang, Juanli Sun, Di Ai, Jian-Ping An, Yuepeng Han

引用次数: 0

A negative feedback cascade controlling jasmonate-induced nicotine biosynthesis revealed by an in-plate nicotine assay. 板内尼古丁测定揭示了一个负反馈级联控制茉莉酸诱导的尼古丁生物合成。

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Molecular Horticulture Pub Date : 2026-09-08 DOI: 10.1186/s43897-026-00249-4

Qing Xu, Ke Xu, Haihui Cao, Yulong Gao, Peng Li, Wenjing Wang, Yong Chen, Michael P Timko, Zhongfeng Zhang, Bingwu Wang, Hongbo Zhang

引用次数: 0