Molecular Horticulture最新文献

Histone deacetylation links jasmonic acid signaling to flower size control in rose. 组蛋白去乙酰化将茉莉酸信号传导与玫瑰花的大小控制联系起来。

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Molecular Horticulture Pub Date : 2026-09-04 DOI: 10.1186/s43897-026-00261-8

Yufei Chang, Jun Lu, Yali Li, Xin Jin, Chunguo Fan, Hmmam Zarif, Guozhen Yuan, Rui Zhou, Hongchi Liu, Jingjing Sun, Changquan Wang, Jinyi Liu

{"title":"Histone deacetylation links jasmonic acid signaling to flower size control in rose.","authors":"Yufei Chang, Jun Lu, Yali Li, Xin Jin, Chunguo Fan, Hmmam Zarif, Guozhen Yuan, Rui Zhou, Hongchi Liu, Jingjing Sun, Changquan Wang, Jinyi Liu","doi":"10.1186/s43897-026-00261-8","DOIUrl":"10.1186/s43897-026-00261-8","url":null,"abstract":"Flower size is a key ornamental trait in roses (Rosa spp.), determined by coordinated regulation of cell proliferation and expansion. Although jasmonic acid (JA) influences plant growth, its role in flower size control remains unclear. Here, we showed that methyl jasmonate (MeJA) treatment reduces flower size in Rosa chinensis, accompanied by elevated expression of the JA-responsive transcription factor RcMYC2. Silencing RcMYC2 resulted in larger flowers due to enhanced petal cell expansion and upregulation of cell expansion-related genes. Mechanistically, RcMYC2 directly bound to the promoter of the expansin gene RcEXPA8 and repressed its transcription via its interaction with the Mediator subunit RcMED25. Consistently, MeJA failed to reduce flower size in RcMED25-silenced plants. Upon JA signal activation, RcMYC2 recruited RcMED25 and the histone deacetylase RcHDA8 to the RcEXPA8 promoter, leading to transcriptional repression via histone deacetylation and restriction of petal cell expansion. These findings demonstrate that histone deacetylation links JA signaling to flower size control, providing new insights into hormone-mediated regulation of organ growth in ornamental plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing cold tolerance through HubZIP6-mediated CBF activation and salicylic acid signaling in pitaya. 通过hubzip6介导的CBF激活和水杨酸信号传导增强火龙果的耐寒性。

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Molecular Horticulture Pub Date : 2026-09-03 DOI: 10.1186/s43897-026-00258-3

Xinglong Hu, Irfan Ali Sabir, Zeliu Xu, Zhike Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin

{"title":"Enhancing cold tolerance through HubZIP6-mediated CBF activation and salicylic acid signaling in pitaya.","authors":"Xinglong Hu, Irfan Ali Sabir, Zeliu Xu, Zhike Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin","doi":"10.1186/s43897-026-00258-3","DOIUrl":"10.1186/s43897-026-00258-3","url":null,"abstract":"Cold stress severely limits the yield and quality of fruit crops, yet its regulatory mechanisms in pitaya remain poorly understood. Here, we identified a cold-inducible bZIP transcription factor, HubZIP6, that plays a central role in enhancing cold tolerance in pitaya. HubZIP6 is a nuclear-localized protein with transcriptional activation activity, and its overexpression in Arabidopsis and tomato significantly improved cold tolerance, as reflected by higher survival rates, reduced ion leakage, and lower reactive oxygen species accumulation. Mechanistically, HubZIP6 directly binds to ACGT motifs in the promoters of HuCBF1 and HuCBF3, thereby activating their expression under cold stress. In addition, HubZIP6 physically interacts with the salicylic acid-binding protein HuSABP2, which synergistically enhances the transcriptional activation of HuCBF genes. Notably, HubZIP6 also directly activates HuSABP2, forming a regulatory loop that connects CBF transcriptional control with salicylic acid signaling. Consistently, overexpression of HuSABP2 further enhances cold tolerance in transgenic plants. Collectively, these findings demonstrate that cold tolerance is enhanced through HubZIP6-mediated integration of CBF activation and salicylic acid signaling, providing a promising genetic target for improving stress resilience in fruit crops.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering podophyllotoxin-mediated defense against downy blight in Litchi chinensis Sonn. via integrated metabolomics and transcriptomics. 解读紫叶毒素介导的荔枝对霜枯病的防御作用。通过综合代谢组学和转录组学。

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Molecular Horticulture Pub Date : 2026-09-02 DOI: 10.1186/s43897-026-00257-4

Hailun Liu, Fachao Shi, Yingjie Wen, Yonghua Jiang, Qian Yan, Hua Huang

{"title":"Deciphering podophyllotoxin-mediated defense against downy blight in Litchi chinensis Sonn. via integrated metabolomics and transcriptomics.","authors":"Hailun Liu, Fachao Shi, Yingjie Wen, Yonghua Jiang, Qian Yan, Hua Huang","doi":"10.1186/s43897-026-00257-4","DOIUrl":"10.1186/s43897-026-00257-4","url":null,"abstract":"Lychee (Litchi chinensis Sonn.) is a commercially important subtropical fruit. Lychee downy blight (LDB), caused by Peronophythora litchii, poses a major threat to lychee production. Here, we identified the LDB-resistant cultivar Yurong (YR) and the susceptible cultivar Guiwei (GW) from 288 global lychee germplasms. Widely targeted metabolomics analysis revealed a striking accumulation of podophyllotoxin (PTOX) in YR. Both in vitro and in vivo assays demonstrated that PTOX effectively inhibited the growth of P. litchii. Further investigation identified LcOMT1 as a key gene encoding an O-methyltransferase (OMT) in the phenylpropanoid pathway. In resistant cultivars, a 773-bp insertion mutation (Hap3 haplotype) in the LcOMT1 promoter enhanced its transcriptional activity. Transgenic approaches using hairy roots in lychee showed that the overexpression of either LcOMT1 or the Hap3 haplotype led to increased PTOX biosynthesis and enhanced resistance to P. litchii. These results demonstrate that the Hap3-mediated upregulation of LcOMT1 drives PTOX accumulation, establishing a crucial defense mechanism against LDB. This study provides new insights into the genetic and metabolic regulatory network underlying lychee resistance to LDB. This study may also lay a theoretical foundation for the development of PTOX-based biopesticides and offer valuable genetic resources for breeding LDB-resistant lychee cultivars.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148875782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual regulation of anthocyanins and proanthocyanidins by PpBL and its linkage to acidity in peach. PpBL对桃花青素和原花青素的双重调控及其与酸度的关联。

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Molecular Horticulture Pub Date : 2026-09-01 DOI: 10.1186/s43897-026-00255-6

Hongyang Xing, Jiaqi Fan, Guizhi Li, Ke Cao, Yong Li, Gengrui Zhu, Weichao Fang, Changwen Chen, Xinwei Wang, Jinlong Wu, Lirong Wang

{"title":"Dual regulation of anthocyanins and proanthocyanidins by PpBL and its linkage to acidity in peach.","authors":"Hongyang Xing, Jiaqi Fan, Guizhi Li, Ke Cao, Yong Li, Gengrui Zhu, Weichao Fang, Changwen Chen, Xinwei Wang, Jinlong Wu, Lirong Wang","doi":"10.1186/s43897-026-00255-6","DOIUrl":"10.1186/s43897-026-00255-6","url":null,"abstract":"Red-fleshed peach exhibits attractive anthocyanin (AN) pigmentation but is often accompanied by undesirable proanthocyanidin (PA)-derived astringency and high fruit acidity (FA), limiting its commercial value. The genetic mechanisms underlying this co-occurrence remain unclear. Using an F1 population derived from 'wen30' and 'wen48', we identified co-localized QTLs for AN, PA, and FA within a 0.32-4.74 Mb region on chromosome 5. Integrating transcriptomics with Y1H, EMSA, and dual-luciferase assays, we demonstrated that PpBL functions as a key regulator of both AN and PA accumulation. PpBL directly activates PpUGT73C3, independently of PpMYB10.1, to promote AN biosynthesis, and enhances PA accumulation by directly upregulating PpLAR and PpANR; PpUGT73C3 also contributes to PA accumulation via an unclear mechanism. In contrast, FA is not directly regulated by PpBL but is associated with a high-acidity PpTST1 allele tightly linked to a blood-TE insertion in the PpBL promoter. The haplotype carrying both the insertion and PpUGT73C3 coding variants confers elevated AN and PA but also increased FA due to linkage drag. These findings reveal pleiotropic control of color and astringency and a linkage-based association with acidity, providing insights for peach breeding.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13531910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148867052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Chromosome‑level genome assembly of Tamarindus indica provides new insights into the evolution of triterpenes and tartaric acid biosynthetic pathway. 校正：柽柳染色体水平的基因组组装为三萜和酒石酸生物合成途径的进化提供了新的见解。

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Molecular Horticulture Pub Date : 2026-08-24 DOI: 10.1186/s43897-026-00256-5

Mitali Singh, Manohar S Bisht, Abhijith M G, Shruti Mahajan, Vineet K Sharma

引用次数: 0

A spatiotemporal atlas of giant-cell formation during Meloidogyne incognita infection in tomato roots. 番茄根中巨细胞形成的时空图谱。

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Molecular Horticulture Pub Date : 2026-08-11 DOI: 10.1186/s43897-026-00272-5

Lijun Jiang, Dan Chen, Qianqian Shi, Tao Huang, Runmao Lin, Jiangzhou Li, Kuai Dai, Chenyang Wang, Wenzheng Ling, Chao Feng, Haichao Cao, Robert Y L Wang, Bingyan Xie, Jinguang Yang

{"title":"A spatiotemporal atlas of giant-cell formation during Meloidogyne incognita infection in tomato roots.","authors":"Lijun Jiang, Dan Chen, Qianqian Shi, Tao Huang, Runmao Lin, Jiangzhou Li, Kuai Dai, Chenyang Wang, Wenzheng Ling, Chao Feng, Haichao Cao, Robert Y L Wang, Bingyan Xie, Jinguang Yang","doi":"10.1186/s43897-026-00272-5","DOIUrl":"10.1186/s43897-026-00272-5","url":null,"abstract":"Root-knot nematodes (RKNs; Meloidogyne spp.) are destructive agricultural parasites, but although giant cell formation is required for establishing parasitism, the mechanism of action has not been fully elucidated. Spatial transcriptomics enables precise spatiotemporal analyses of gene expression, facilitating studies of cell heterogeneity. We performed spatial transcriptomic sequencing on Moneymaker tomato root galls caused by M. incognita infection at 3, 5, and 7 days post-inoculation to investigate RKN-induced giant cell formation. Five major cell types were identified; of these, giant cell clusters were localized predominantly in the xylem, stele, and meristem. Four novel giant cell-specific marker genes were confirmed through RNA in situ hybridization. Pseudotime analysis revealed genes potentially associated with giant cell formation. Virus-induced gene silencing (VIGS) of four genes encoding a cyclin-dependent kinase, two cell division cycle-associated proteins, and a MYB3R-1-like transcription factor-hypothesized to maintain the cell cycle or gene expression during mitosis-resulted in significantly fewer galls and significantly smaller giant cells. This study established the first spatiotemporal atlas of RKN-infected tomato roots and identified genes associated with giant cell formation, laying a foundation for further research on the establishment of RKN feeding sites, providing novel insights into RKN pathogenic mechanisms, and potentially guiding novel control strategies.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148713821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lily NIN-like transcription factor LlNLP7 confers thermotolerance and promotes growth. 百合蛋白样转录因子LlNLP7具有耐热性并促进生长。

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Molecular Horticulture Pub Date : 2026-08-10 DOI: 10.1186/s43897-026-00253-8

Ziwei Liao, Jun Xiang, Yinyi Zhang, Xue Gong, Ze Wu, Nianjun Teng

引用次数: 0

Designing rapid-cycling, compact architecture in tomato for vertical farming. 为垂直种植设计快速循环、紧凑的番茄结构。

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Molecular Horticulture Pub Date : 2026-08-07 DOI: 10.1186/s43897-026-00248-5

Yoonseo Lim, Jeong-Tak An, So Jin Park, Yeeun Han, Ho-Young Jeong, Chanhui Lee, Soon Ju Park, Dae-Hyun Jung, Choon-Tak Kwon

引用次数: 0

Artificial autotetraploidy modulates flowering regulation in industrial hemp through DNA methylation and transcriptomic reprogramming. 人工自四倍体通过DNA甲基化和转录组重编程调节工业大麻的开花调节。

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Molecular Horticulture Pub Date : 2026-08-06 DOI: 10.1186/s43897-026-00245-8

Chen Kuang, Ying Xu, Boyang Liu, Chaohua Cheng, Canhui Deng, Jianguang Su, Xiaoyu Zhang, Zhigang Dai, Zemao Yang, Jiquan Chen, Ying Xu, Qing Tang

引用次数: 0

A VvWRKY8-salicylic acid pathway amplification loop confers resistance to Plasmopara viticola in grapevine. 一个vvwrky8 -水杨酸途径扩增环赋予葡萄对葡萄浆原菌的抗性。

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Molecular Horticulture Pub Date : 2026-08-05 DOI: 10.1186/s43897-026-00233-y

Huimin Huang, Junjie Qu, Jiaqi Liu, Wei Wu, Jiang Lu

{"title":"A VvWRKY8-salicylic acid pathway amplification loop confers resistance to Plasmopara viticola in grapevine.","authors":"Huimin Huang, Junjie Qu, Jiaqi Liu, Wei Wu, Jiang Lu","doi":"10.1186/s43897-026-00233-y","DOIUrl":"10.1186/s43897-026-00233-y","url":null,"abstract":"To defend against pathogen invasion, plants deploy a variety of strategies, among which salicylic acid (SA), a key plant defense hormone, plays crucial roles in enhancing host resistance to biotrophic and semibiotrophic microbes. Although numerous studies elucidated mechanisms of the SA signaling pathway, many questions remain. In this study, we show that the group IIc WRKY transcription factor VvWRKY8 is involved in grape (Vitis vinifera) defense responses to the oomycete pathogen Plasmopara viticola, as indicated by transcriptome analyses. VvWRKY8 increases the expression of defense-related genes and SA accumulation, thereby promoting grape resistance to P. viticola. Further analyses reveal that VvWRKY8 is recruited to the promoters of VvCBP60g and VvSARD1, which encode two key regulators of SA biosynthesis, and upregulates their transcription. Moreover, VvWRKY8 transcription is induced by SA and by two bZIP transcription factors, VvTGA2a and VvTGA2b, which cooperate with the SA receptors VvNPR1 and VvNPR3 to modulate the expression of SA-responsive genes. Collectively, our results indicate that a positive feedback loop involving VvWRKY8 and the SA pathway components VvCBP60g, VvSARD1, and VvTGA2a/2b functions in response to P. viticola attack in grapevine.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13440096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0