Molecular Horticulture最新文献_第2页

Chloroplast acetyltransferase GNAT2 acts as a redox-regulated switch for state transitions in tomato. 番茄叶绿体乙酰转移酶GNAT2是氧化还原调控的状态转换开关。

IF 8.1

Molecular Horticulture Pub Date : 2025-08-06 DOI: 10.1186/s43897-025-00164-0

Xiaoyun Wang, Jianghao Wu, Hongxin Li, Ying Liu, Dexian Han, Danhui Dong, Jialong Zhang, Lixin Zhang, Na Zhang, Yang-Dong Guo

{"title":"Chloroplast acetyltransferase GNAT2 acts as a redox-regulated switch for state transitions in tomato.","authors":"Xiaoyun Wang, Jianghao Wu, Hongxin Li, Ying Liu, Dexian Han, Danhui Dong, Jialong Zhang, Lixin Zhang, Na Zhang, Yang-Dong Guo","doi":"10.1186/s43897-025-00164-0","DOIUrl":"10.1186/s43897-025-00164-0","url":null,"abstract":"State transition is a dynamic process to balance the amount of light energy received by photosystem I (PSI) and photosystem II (PSII) so as to maintain an optimal photosynthetic yield and to minimize photo-damage in a fluctuating light environment. Recent studies show that chloroplast acetyltransferase participates in the acetylation of photosynthetic proteins and state transitions. However, the exact molecular mechanisms are poorly understood. In this study, we characterized a chloroplast acetyltransferase in Solanum lycopersicum, SlGNAT2, and found that mutants lacking this enzyme are deficient in state transitions and retarded in growth under fluctuating light. Acetyltransferase activity assays and fluorescence measurements suggest that 6Lys of mature SlLhcb2 protein is a target of SlGNAT2 and might be involved in state transitions. In addition, 131Cys-related redox changes of SlGNAT2 affect its acetylation activity on SlLhcb2 and influence the assembly of the PSI-LHCI-LHCII supercomplex. Therefore, we propose that the chloroplast redox state may regulate the activity of SlGNAT2 which in turn acetylates SlLhcb2 and mediates state transitions in higher plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"39"},"PeriodicalIF":8.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144790146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative metabolome and genome-wide transcriptome analyses reveal the regulatory network for bioactive compound biosynthesis in lettuce upon UV-A radiation. 综合代谢组和全基因组转录组分析揭示了UV-A辐射下生菜生物活性化合物生物合成的调控网络。

IF 8.1

Molecular Horticulture Pub Date : 2025-08-05 DOI: 10.1186/s43897-025-00163-1

Lingyan Zha, Shiwei Wei, Xiao Yang, Qingliang Niu, Danfeng Huang, Jingjin Zhang

{"title":"Integrative metabolome and genome-wide transcriptome analyses reveal the regulatory network for bioactive compound biosynthesis in lettuce upon UV-A radiation.","authors":"Lingyan Zha, Shiwei Wei, Xiao Yang, Qingliang Niu, Danfeng Huang, Jingjin Zhang","doi":"10.1186/s43897-025-00163-1","DOIUrl":"10.1186/s43897-025-00163-1","url":null,"abstract":"Ultraviolet A (UV-A) radiation possesses great potential for enhancing the bioactive properties of vegetables and also has promising application prospects in controlled-environment agriculture. Lettuce is a widely cultivated model vegetable in controlled-environment agriculture with abundant health-beneficial bioactive compounds. However, the comprehensive regulatory effectiveness and mechanism of UV-A on bioactive compounds in lettuce remain largely unclear. To address this issue, we performed transcriptomic and metabolomic analyses of UV-A-treated lettuce to construct a global map of metabolic features and transcriptional regulatory networks for all major bioactive compounds. Our study revealed that UV-A promotes the accumulation of most phenylpropanoids and vitamins (provitamin A and vitamin E/K1/B6) but represses the biosynthesis of sesquiterpenoids. MYB transcription factors (TFs) are key activators of bioactive compound biosynthesis promoted by UV-A, whereas WRKY TFs primarily inhibit the production of sesquiterpenoids. Moreover, light signaling plays a crucial and direct regulatory function in stimulating the biosynthesis of phenylpropanoids and vitamins but not in that of sesquiterpenoids. In comparison, hormone signaling dominates a more decisive regulatory role in repressing sesquiterpenoid biosynthesis through working directly and interacting with WRKY TFs. This study paves the way toward an understanding of the bioactive compound regulation and genetic improvement of lettuce bioactivity value.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"45"},"PeriodicalIF":8.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two gap-free genomes of Argentina provide insights into their genetic relationships. 阿根廷的两个无间隙基因组提供了对它们的遗传关系的见解。

IF 8.1

Molecular Horticulture Pub Date : 2025-08-04 DOI: 10.1186/s43897-025-00160-4

Xien Wu, Qin Qiao, Qiang Cao, Zhongqiong Tian, La Qiong, Ticao Zhang

引用次数: 0

A single nucleotide substitution in the SlMCT gene contributes to great morphological alternations in tomato. SlMCT基因的单核苷酸替换导致了番茄的形态变化。

IF 8.1

Molecular Horticulture Pub Date : 2025-08-01 DOI: 10.1186/s43897-025-00159-x

Mengyi Yu, Yinge Xie, Zilin Qian, Yu Zhong, Huolin Shen, Wencai Yang

{"title":"A single nucleotide substitution in the SlMCT gene contributes to great morphological alternations in tomato.","authors":"Mengyi Yu, Yinge Xie, Zilin Qian, Yu Zhong, Huolin Shen, Wencai Yang","doi":"10.1186/s43897-025-00159-x","DOIUrl":"10.1186/s43897-025-00159-x","url":null,"abstract":"Terpenoids, a group of metabolites, are important to plant development and color formation, and serve as valuable nutrients for humans. The enzyme 4-diphosphocytidyl- 2 C-methyl-D-erythritol cytidyltransferase (MCT) plays a pivotal role in the methylerythritol phosphate (MEP) pathway for terpenoid biosynthesis. However, the potential lethality of MCT mutants has hindered further exploration into its functional role in terpenoid metabolite families in plants. Here, we characterized a rare MCT mutant yfm with dwarfism, chlorosis, small leaves, and yellow fruits in tomato. Map-based cloning and sequence analysis revealed that a single nucleotide substitution in the SlMCT gene, which resulted in a point mutation (Leu297Pro) in amino acid in the mutant. Over-expression and complementation of the wild-type SlMCTT in the yfm mutant restored the fruit color and the other defective phenotypes. This mutation altered the gene expressions and metabolic components in the MEP and other pathways. Consequently, the total contents of carotenoids, chlorophyll, IAA, GAs, and SA were decreased, while the contents of CK, JA, and ABA were increased. Eventually, these alterations led to changes in plant phenotypes and fruit color in yfm. These findings provide novel insights into understanding the roles of MCT on plant development and pigment biosynthesis.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"49"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12315435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoplastic proteomic reveals Colletotrichum fructicola effector CfXyn11A recognized by tobacco and suppressed by pear in the apoplast. 外质体蛋白质组学揭示了烟草识别、梨抑制的炭疽菌外质体效应因子CfXyn11A。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-07 DOI: 10.1186/s43897-025-00161-3

Chenyang Han, Shutian Tao, Zhihua Xie, Fengquan Liu, Shaoling Zhang

{"title":"Apoplastic proteomic reveals Colletotrichum fructicola effector CfXyn11A recognized by tobacco and suppressed by pear in the apoplast.","authors":"Chenyang Han, Shutian Tao, Zhihua Xie, Fengquan Liu, Shaoling Zhang","doi":"10.1186/s43897-025-00161-3","DOIUrl":"10.1186/s43897-025-00161-3","url":null,"abstract":"Colletotrichum fructicola is a hemibiotrophic fungal plant pathogen that transitions from biotrophic growth on living host tissue to necrotrophic tissue destruction. During the hemibiotrophic phase, numerous proteins are secreted into the apoplast, mediating host‒pathogen interactions. In this study, we employed apoplastic proteomics and RNA-seq to analyse the proteins secreted during the interaction between C. fructicola and pear. A secreted xylanase, CfXyn11A, was identified as a dual-function effector. In the nonhost Nicotiana benthamiana, it triggered immune responses, including reactive oxygen species production and programmed cell death. However, CfXyn11A evades detection in the host pear, enabling its role in cell wall degradation and nutrient acquisition. Genetic and biochemical assays confirmed that the immune-triggering function of CfXyn11A relies on its apoplastic localization and is independent of enzymatic activity. Additionally, we identified an aspartic protease-like protein, PbXIP1, in the pear apoplast, which binds CfXyn11A to suppress its enzymatic activity and virulence. This study highlights the role of apoplastic proteomics in elucidating the molecular mechanisms underlying plant immunity and pathogen virulence and emphasizes the contrasting outcomes of CfXyn11A in different host contexts. The findings provide new insights into the interplay between extracellular effectors and plant defense proteins during fungal infection.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"42"},"PeriodicalIF":10.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PeachMD: a multi-omics database for peach. PeachMD：桃子的多组学数据库。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-04 DOI: 10.1186/s43897-025-00157-z

Ang Li, Shihang Sun, Hongmei Wang, Akhi Badrunnesa, Junren Meng, Xiongwei Li, Yuan Gao, Liang Niu, Lei Pan, Wenyi Duan, Guochao Cui, Zhiqiang Wang, Wenfang Zeng

引用次数: 0

A subgroup I bZIP transcription factor PpbZIP18 plays an important role in sucrose accumulation in peach. bZIP转录因子PpbZIP18亚群在桃的蔗糖积累中起重要作用。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-03 DOI: 10.1186/s43897-025-00156-0

Xian Zhang, Wen Xiao, Yudi Liu, Yunpeng Cao, Ruo-Xi Zhang, Yuepeng Han

引用次数: 0

Breeding a novel cauliflower with exceptional fragrance. 培育出香味独特的新型花椰菜。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-02 DOI: 10.1186/s43897-025-00178-8

Xiaoli Zhang, Xiaoxu Li, Long Chen, Zhenghua Wen, Fengqing Han, Daping Gong, Minmin Xie, Zhe Zhao, Yu Zhao, Wei Zhang, Mingli Chen, Zhiyuan Li

引用次数: 0

Metabolomic and evolutionary integration unveils medicinal potential in six Corydalis species. 代谢组学和进化整合揭示了六种延胡索属植物的药用潜力。

IF 10.6

Molecular Horticulture Pub Date : 2025-07-01 DOI: 10.1186/s43897-025-00162-2

Yun Gao, Xiangyu Zhou, Mengxiao Yan, Zhengwei Wang, Xin Zhong, Xiaochen Li, Junjie Zhu, Yu Kong, Wanrong Zhu, Ruolin Geng, Yaping Zhou, Qing Zhao, Yonghong Hu, Ping Xu

{"title":"Metabolomic and evolutionary integration unveils medicinal potential in six Corydalis species.","authors":"Yun Gao, Xiangyu Zhou, Mengxiao Yan, Zhengwei Wang, Xin Zhong, Xiaochen Li, Junjie Zhu, Yu Kong, Wanrong Zhu, Ruolin Geng, Yaping Zhou, Qing Zhao, Yonghong Hu, Ping Xu","doi":"10.1186/s43897-025-00162-2","DOIUrl":"10.1186/s43897-025-00162-2","url":null,"abstract":"The Corydalis genus, one of the largest within the Papaveraceae family, holds a rich diversity of medicinal resources, particularly among its tuberous species. Benzylisoquinoline alkaloids (BIAs) are primarily responsible for the medicinal properties observed in Corydalis plants. In this study, we conducted an integrated evolutionary analysis by combining whole-genome resequencing with comprehensive metabolite profiling across various Corydalis species. Guided by these initial findings, supported by local cultivation practices and an extensive literature review, we further investigated six tuberous Corydalis species: C. yanhusuo, C. decumbens, C. schanginii, C. ledebouriana, C. solida, and the newly identified C. nanchuanensis. Our results revealed conserved alkaloid profiles across these species but highlighted significant variations in key bioactive compounds. Notably, C. nanchuanensis exhibited considerably higher levels of tetrahydropalmatine compared to the commonly used medicinal species C. yanhusuo, while C. solida, originally sourced from the Netherlands, displayed elevated concentrations of corydaline, palmatine, and dehydrocorydaline. Additionally, transcriptome-metabolome correlation analyses pinpointed several critical genes involved in protopine biosynthesis, particularly emphasizing the TNMT gene family. These discoveries significantly enhance our understanding of metabolic diversity in tuberous Corydalis, providing essential insights for the exploration of novel medicinal resources and facilitating targeted genetic improvements for therapeutic use.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"38"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12211431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening. 肉质果实成熟过程中显性激素信号产生的触发机制。

IF 10.6

Molecular Horticulture Pub Date : 2025-06-06 DOI: 10.1186/s43897-025-00155-1

Jinyao Ouyang, Bing He, Ya Zeng, Changsheng Zhai, Yating Li, Jie Li, Pingyin Guan, Wensuo Jia

{"title":"The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening.","authors":"Jinyao Ouyang, Bing He, Ya Zeng, Changsheng Zhai, Yating Li, Jie Li, Pingyin Guan, Wensuo Jia","doi":"10.1186/s43897-025-00155-1","DOIUrl":"10.1186/s43897-025-00155-1","url":null,"abstract":"Fleshy Fruit (FF) ripening is regulated by multiple hormones, which can be categorized into two groups, i.e., the positive signals acting to promote FF ripening and the negative signals acting to suppress FF ripening. Ethylene (ET) and abscisic acid (ABA) are two predominant positive signals respectively controlling climacteric (CL) and non-climacteric (NC) FF ripening, whereas auxin (IAA) is the predominant negative signal controlling both FF growth and ripening. Functioning of these hormones is initiated by an alteration of the hormonal levels, which is referred to as the process of Hormonal Signal Production (HSP) in FF development and ripening. While the hormonal regulation of FF ripening has been extensively studied and reviewed, knowledge of HSP has never been summarized and discussed. The purpose of this review is to summarize and discuss the triggering mechanism of HSP. We first summarize the physiological, biochemical and molecular bases of HSP for three crucial hormones, ET, ABA, and IAA, including hormonal metabolism, transport and reciprocal regulation of HSP among different hormones, we then summarize and discuss the recent discoveries on the mechanism of cellular signal transduction of HSP. Finally, we propose several viewpoints to facilitate comprehension of the future research endeavors.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"35"},"PeriodicalIF":10.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0