Molecular Horticulture最新文献_第2页

Comparative genomics and time-course transcriptomics uncover homoeologous exchange events reshaping glucosinolate metabolism in Brassica napus. 比较基因组学和时间过程转录组学揭示了重塑甘蓝型油菜硫代糖苷代谢的同源交换事件。

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Molecular Horticulture Pub Date : 2026-08-04 DOI: 10.1186/s43897-026-00241-y

Yizhou He, Zetao Bai, Zengfeng Wang, Yu-Xuan Bai, Qinlin Ke, Fan Liu, Chaobo Tong, Aixia Gu, Marta Francisco, Deyang Xu, Lijiang Liu, Qing-Yong Yang, Shengyi Liu, Wen-Hui Lin, Yuanyuan Zhang

{"title":"Comparative genomics and time-course transcriptomics uncover homoeologous exchange events reshaping glucosinolate metabolism in Brassica napus.","authors":"Yizhou He, Zetao Bai, Zengfeng Wang, Yu-Xuan Bai, Qinlin Ke, Fan Liu, Chaobo Tong, Aixia Gu, Marta Francisco, Deyang Xu, Lijiang Liu, Qing-Yong Yang, Shengyi Liu, Wen-Hui Lin, Yuanyuan Zhang","doi":"10.1186/s43897-026-00241-y","DOIUrl":"10.1186/s43897-026-00241-y","url":null,"abstract":"Improving glucosinolate (GSL) profiles in rapeseed (Brassica napus)-high in leaves for pathogen resistance but low in seeds for meal quality-is a key breeding goal, yet its genetic basis remains unclear. Here, we present a chromosome-level genome assembly for ZY821, an elite high-GSL variety, generated using long-read sequencing and Hi-C scaffolding. Comparative analysis with the low-GSL variety ZS11 identified three major homoeologous exchange (HE) events and extensive structural variation. Notably, an A09-C09 HE event replaced the low-expression BnaC09.MYB28 allele with the high-expression BnaA09.MYB28 allele, resulting in elevated MYB28s expression and thereby increased GSL accumulation in ZY821, whereas a deletion of BnaA09.MYB28 in ZS11 significantly reduced the expression of multiple putative downstream targets in the GSL biosynthesis pathway, leading to a reduction in GSL content. This mechanism was supported by population-level HE analysis and time-course transcriptomes across 116 RNA-Seq samples. Furthermore, joint differential expression and co-expression network analyses uncovered several novel candidate genes implicated in GSL metabolism. Collectively, our study provides new mechanistic insights into the genetic control of GSL accumulation, with significant implications for breeding optimized GSL profiles.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13435445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148669630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A MYB-WRKY feedback module activates MdAAT2‑like to regulate aromatic ester biosynthesis during apple ripening. MYB-WRKY反馈模块激活MdAAT2 - like调控苹果成熟过程中芳香酯的生物合成。

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Molecular Horticulture Pub Date : 2026-08-03 DOI: 10.1186/s43897-026-00240-z

Bin Wang, Fang Zhou, Yingrui Gao, Min Cai, Xiaoxiao He, Lulong Sun, Zhengyang Zhao

{"title":"A MYB-WRKY feedback module activates MdAAT2‑like to regulate aromatic ester biosynthesis during apple ripening.","authors":"Bin Wang, Fang Zhou, Yingrui Gao, Min Cai, Xiaoxiao He, Lulong Sun, Zhengyang Zhao","doi":"10.1186/s43897-026-00240-z","DOIUrl":"10.1186/s43897-026-00240-z","url":null,"abstract":"Aromatic esters are key determinants of apple fruit aroma and consumer preference. Here, we investigated the molecular mechanisms underlying ester biosynthesis by characterizing MdAAT2-like, a critical ester-synthesizing gene in apple. MdAAT2-like expressions were significantly upregulated during fruit ripening and positively correlated with ester accumulation. Functional validation in apple and tomato demonstrated that MdAAT2-like overexpression enhanced ester contents, while silencing or knockout reduced ester production. Enzymatic assays revealed that MdAAT2-like exhibits higher catalytic efficiency for medium- and short-chain acyl-CoAs compared to other AAT family members. Two transcription factors, MdMYB98-like and MdWRKY21, were identified as direct activators of MdAAT2-like. Both factors bind its promoter and synergistically enhance transcription through protein interaction. Notably, we uncovered a positive feedback model wherein MdMYB98-like and MdWRKY21 reciprocally activate each other's expression, reinforcing rapid ester synthesis during ripening. This study reveals a MYB-WRKY regulatory module controlling ester biosynthesis in apple.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13430851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148664703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving heterologous protein production by modulating ROS homeostasis in Nicotiana benthamiana. 通过调节本烟活性氧稳态改善异源蛋白的产生。

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Molecular Horticulture Pub Date : 2026-08-02 DOI: 10.1186/s43897-026-00260-9

Yuanxin Wu, Qi Ni, Sayed Abdul Akher, Zenglin Zhang, Jie Wang, Yongfeng Guo, Jianfeng Zhang

引用次数: 0

Dynamic chromatin accessibility reveals BrKAN2 as a key regulator of Chinese cabbage leaf heading. 动态染色质可及性揭示了BrKAN2是白菜叶片抽穗的关键调控因子。

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Molecular Horticulture Pub Date : 2026-07-08 DOI: 10.1186/s43897-026-00239-6

Huiling Guo, Fengming Li, Lupeng Zhang, Yuanyuan Zhang, Xu Cai, Haixu Chen, Xiaoxiao Zheng, Jian Wu, Xiaowu Wang, Jianli Liang

{"title":"Dynamic chromatin accessibility reveals BrKAN2 as a key regulator of Chinese cabbage leaf heading.","authors":"Huiling Guo, Fengming Li, Lupeng Zhang, Yuanyuan Zhang, Xu Cai, Haixu Chen, Xiaoxiao Zheng, Jian Wu, Xiaowu Wang, Jianli Liang","doi":"10.1186/s43897-026-00239-6","DOIUrl":"10.1186/s43897-026-00239-6","url":null,"abstract":"Chinese cabbage forms a leafy head as its main edible organ, a process involving extensive morphological and transcriptional changes. Here, we employed ATAC-seq to profile a time-series chromatin accessibility landscape across key developmental stages of leaf heading. This analysis revealed highly dynamic, stage-specific chromatin accessible regions accompanied by distinct transcription factors activities. Genes associated with adaxial-abaxial polarity and hormone signaling displayed dynamic accessibility patterns, suggesting their critical roles in head formation. BrKAN2 emerged as a key candidate regulator. Mutant analysis in Chinese cabbage and overexpression in Arabidopsis significantly altered leaf morphology and dorsoventral polarity. Population genetic analysis further indicated strong selection on BrKAN2 in heading Brassica rapa. Integration of DAP-seq and ATAC-seq identified BrKAN2.1 target genes enriched in organ development and hormone-related pathways, which were validated by VIGS and EMSA. Auxin responsiveness assays underscored the importance of auxin signaling in heading. Collectively, these findings uncover a dynamic chromatin landscape underlying leaf heading and establish BrKAN2 as a central regulatory factor, offering new insights for genetic improvement of Chinese cabbage.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13343676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148406148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A miR858 variant negatively regulates resistance to tea leaf spot through targeting the CsMYB1-CsPME41 module. miR858变体通过靶向CsMYB1-CsPME41模块负向调节对茶叶病的抗性。

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Molecular Horticulture Pub Date : 2026-07-07 DOI: 10.1186/s43897-026-00238-7

Dongqin Zhang, Bin Wang, Zeqi Qi, Jun Zhang, Yu Huang, Delu Wang, Baoan Song, Zhuo Chen

{"title":"A miR858 variant negatively regulates resistance to tea leaf spot through targeting the CsMYB1-CsPME41 module.","authors":"Dongqin Zhang, Bin Wang, Zeqi Qi, Jun Zhang, Yu Huang, Delu Wang, Baoan Song, Zhuo Chen","doi":"10.1186/s43897-026-00238-7","DOIUrl":"10.1186/s43897-026-00238-7","url":null,"abstract":"In recent years, tea leaf spot, caused by the fungus Epicoccum sorghinum, adversely affects both the yield and quality of tea in China. Transient overexpression and antisense oligonucleotide (AsODN) assays revealed that upregulating CsMYB1, a transcription factor located in the nucleus, enhances resistance to E. sorghinum infection in tea leaves. Overexpression of CsMYB1 in transgenic Nicotiana benthamiana conferred resistance to Botrytis cinerea. Multi-omics assays of transiently overexpressed CsMYB1 in tea leaves indicated that CsMYB1 induces the expression of numerous disease resistance genes. DNA affinity purification sequencing assay indicated that CsMYB1 can activate the expression of the disease resistance gene pectinesterase/pectinesterase inhibitor 41 (CsPME41). β-glucuronidase and dual-luciferase assays showed that csi-miR858-3p_L-1 targets and cleaves CsMYB1. Moreover, transient overexpression and AsODN assays in tea leaves and transgenic csi-miR858-3p_L-1 N. benthamiana plants indicated that increasing csi-miR858-3p_L-1 levels heightens susceptibility to E. sorghinum in tea leaves. The relative expression levels of CsMYB1, CsPME41, and csi-miR858-3p_L-1 in tea leaves exhibited distinct spatial and temporal patterns in response to E. sorghinum invasion. This study reveals that the csi-miR858-3p_L-1-CsMYB1-CsPME41 module plays a role in the disease resistance response of tea plants to E. sorghinum infection, providing crucial data for resistance breeding.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13339706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148399520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbivore-induced stomatal closure in tomato is mediated by a Ca²⁺-dependent GLR-CPK27-SLAC1 pathway. 草食动物诱导的番茄气孔关闭是由Ca2 +依赖的GLR-CPK27-SLAC1途径介导的。

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Molecular Horticulture Pub Date : 2026-07-06 DOI: 10.1186/s43897-026-00237-8

Huijia Kang, Xinyi Wu, Chufan Xue, Xinyan Li, Yuhui Jia, Yanhong Zhou, Jingquan Yu, Chaoyi Hu

引用次数: 0

Non-coding RNA: a new perspective on the regulation of secondary metabolites in medicinal plants. 非编码RNA：药用植物次生代谢调控的新视角。

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Molecular Horticulture Pub Date : 2026-07-04 DOI: 10.1186/s43897-026-00259-2

Shuaibiao Zhang, Susu Chen, Lei Zhang

{"title":"Non-coding RNA: a new perspective on the regulation of secondary metabolites in medicinal plants.","authors":"Shuaibiao Zhang, Susu Chen, Lei Zhang","doi":"10.1186/s43897-026-00259-2","DOIUrl":"10.1186/s43897-026-00259-2","url":null,"abstract":"Medicinal plants are a valuable reservoir of diverse secondary metabolites (SMs), which serve as essential sources for natural drug development. However, the natural production of these bioactive compounds is generally low. Moreover, the biosynthetic processes of these SMs are regulated by a sophisticated network involving transcription factors, post-translational modifications, as well as plant hormones and environmental factors. Recent studies have identified an increasing number of non-coding RNAs (ncRNAs) and confirmed their roles in regulating SM biosynthesis. In this review, we systematically summarize the research progress on the regulation of SM biosynthesis by ncRNAs in medicinal plants, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and phased secondary siRNAs (phasiRNAs). Additionally, we summarize ncRNA‑mediated studies in staple crops, horticultural plants, and woody species to provide references for medicinal plant research. Finally, we discuss future research directions for ncRNAs in medicinal plants, including the construction of spatiotemporal regulatory networks, the application of artificial intelligence (AI)-assisted approaches, the elucidation of molecular regulatory mechanisms, and potential applications of ncRNAs in the standardized production of medicinal plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-species transfer of sensor and helper NLR confers resistance to black rot in Brassica oleracea. 传感器和辅助NLR的跨种转移赋予甘蓝对黑腐病的抗性。

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Molecular Horticulture Pub Date : 2026-07-03 DOI: 10.1186/s43897-026-00235-w

Siping Deng, Xiaofei Du, Hongxue Ma, Limei Yang, Mu Zhuang, Yong Wang, Jialei Ji, Yangyong Zhang, Hailong Guo, Honghao Lv

引用次数: 0

Regulation of the growth-to-ripening transition in tomato fruits by energy charge involving SlATP-PRT and SlAPRT1. SlATP-PRT和SlAPRT1参与的能量电荷对番茄果实生长到成熟转变的调控

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Molecular Horticulture Pub Date : 2026-07-02 DOI: 10.1186/s43897-026-00234-x

Ye Liu, Peiwen Wu, Bowen Li, Zheng Bian, Guiqin Qu, Daqi Fu, Hongliang Zhu, Yunbo Luo, Weihao Wang, Benzhong Zhu

{"title":"Regulation of the growth-to-ripening transition in tomato fruits by energy charge involving SlATP-PRT and SlAPRT1.","authors":"Ye Liu, Peiwen Wu, Bowen Li, Zheng Bian, Guiqin Qu, Daqi Fu, Hongliang Zhu, Yunbo Luo, Weihao Wang, Benzhong Zhu","doi":"10.1186/s43897-026-00234-x","DOIUrl":"10.1186/s43897-026-00234-x","url":null,"abstract":"The lifecycle of tomato fruit mainly comprises two distinct phases: the initial growth and development phase, followed by the ripening and senescence phase. However, the mechanism initially triggering the transition from an energy-intensive growth phase to the ripening phase remains unclear. First, we found that the fruit energy charge was significantly reduced before this transition, which might be caused by elevated levels of adenosine monophosphate (AMP) and histidine (His). Second, we constructed transgenic tomato plants for the key enzymes in the AMP salvage pathway (SlAPRT1) and His biosynthesis pathway (SlATP-PRT) via genome editing and overexpression. All transgenic plants caused a severe inhibition of fruit ripening, which was consistent with exogenous AMP treatment on wild-type plants, primarily due to the increased energy charge. Particularly, Slatp-prt mutant fruits could hardly produce ethylene or initiate ripening, but exogenous His treatment could restore their energy charge and ripening initiation. Finally, reducing the energy charge via exogenous His treatment also effectively accelerated the growth-to-ripening transition of wild-type fruits both on the plant and post-harvest. In conclusion, this study reveals that low energy charge and high His levels co-trigger the growth-to-ripening transition of tomato fruits, providing novel and valuable insights into the mechanisms underlying ripening initiation.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13326308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148370034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DcLcyB1 and DcLcyB2, two lycopene β-cyclases with partial functional overlap modulate carotene profiles in carrot roots via distinct catalytic properties. DcLcyB1和DcLcyB2是两个功能部分重叠的番茄红素β环化酶，它们通过不同的催化性质调节胡萝卜素在胡萝卜根中的分布。

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Molecular Horticulture Pub Date : 2026-07-01 DOI: 10.1186/s43897-026-00232-z

Ya-Hui Wang, Pei-Zhuo Liu, Rong-Rong Zhang, Yu-Qing Zhang, Hui-Ru Wang, Yu-Jie Sun, Jing Ma, Zhi-Sheng Xu, Ai-Sheng Xiong

{"title":"DcLcyB1 and DcLcyB2, two lycopene β-cyclases with partial functional overlap modulate carotene profiles in carrot roots via distinct catalytic properties.","authors":"Ya-Hui Wang, Pei-Zhuo Liu, Rong-Rong Zhang, Yu-Qing Zhang, Hui-Ru Wang, Yu-Jie Sun, Jing Ma, Zhi-Sheng Xu, Ai-Sheng Xiong","doi":"10.1186/s43897-026-00232-z","DOIUrl":"10.1186/s43897-026-00232-z","url":null,"abstract":"In plant, lycopene β-cyclase is a crucial enzyme in carotenoid metabolic pathway, which can induce color alteration. Carrots (Daucus carota) possess two genes encoding lycopene β-cyclase, namely DcLcyB1 and DcLcyB2. Little is known regarding the functional disparities between these two proteins in regulating carrot carotenoid accumulation. We found that the expression level of DcLcyB2 was higher than that of DcLcyB1 in carrot roots. Enzyme reaction in E. coli demonstrated that both two DcLcyB proteins were capable of cycloconverting lycopene to β-carotene, but DcLcyB2 tended to have a higher preference for monocyclic carotene substrates, resulting in more α-carotene production. After the DcLcyBs were separately overexpressed in red carrots, the roots turned yellow, accompanied by the reduction of lycopene and β-carotene content and the entire carotenoid metabolism flowed downstream towards xanthophylls. The expression levels of DcCHXE, DcCYP97A3, DcCHXB1 and DcCHXB2 in the DcLcyB-OE lines raised sharply. After gene editing of DcLcyB1/2, the α-/β-carotene ratio changed conspicuously, particularly in the dclcyb2 mutants, where α-carotene content dropped sharply, while β-carotene remained high. The expression levels of most structural genes in carotenoid pathway responded dynamically. Our results enriched the understanding of functionally redundant but differentiated roles of two DcLcyB isoenzymes in carrot root coloring.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13321510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148362714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0