Yajun Yan, Wanwan Zhang, You Wang, Yue Wang, Chuanwei Li, Nan Zhao, Lijie Zhou, Jiangshuo Su, Likai Wang, Jiafu Jiang, Sumei Chen, Fadi Chen
{"title":"CmHRE2L-CmACS6 transcriptional cascade negatively regulates waterlogging tolerance in Chrysanthemum.","authors":"Yajun Yan, Wanwan Zhang, You Wang, Yue Wang, Chuanwei Li, Nan Zhao, Lijie Zhou, Jiangshuo Su, Likai Wang, Jiafu Jiang, Sumei Chen, Fadi Chen","doi":"10.1186/s43897-024-00138-8","DOIUrl":"10.1186/s43897-024-00138-8","url":null,"abstract":"<p><p>The role of ethylene as an initial signaling molecule in waterlogging stress is well-established. However, the complex molecular mechanisms underlying ethylene biosynthesis and its functional significance in chrysanthemums under waterlogging conditions have remained unclear. In this study, we observed an increase in the expression of 1-aminocyclopropane-1-carboxylate synthase 6 (CmACS6), which encodes a key enzyme responsible for ethylene biosynthesis, in response to waterlogging. This elevation increases ethylene production, induces leaf chlorosis, and enhances the chrysanthemum's sensitivity to waterlogging stress. Moreover, our analysis of upstream regulators revealed that the expression of CmACS6, in response to waterlogging, is directly upregulated by CmHRE2-like (Hypoxia Responsive ERF-like, CmHRE2L), an ethylene response factor. Notably, CmHRE2-L binds directly to the GCC-like motif in the promoter region of CmACS6. Genetic validation assays demonstrated that CmHRE2L was induced by waterlogging and contributed to ethylene production, consequently reducing waterlogging tolerance in a partially CmACS6-dependent manner. This study identified the regulatory module involving CmHRE2L and CmACS6, which governs ethylene biosynthesis in response to waterlogging stress.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"15"},"PeriodicalIF":10.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sen Chen, Liang Xu, Yan Wang, Baozhen Mao, Xiaoli Zhang, Qiyu Song, Feng Cui, Yingbo Ma, Junhui Dong, Kai Wang, Hongyu Bi, Liwang Liu
{"title":"RsWRKY40 coordinates the cold stress response by integrating RsSPS1-mediated sucrose accumulation and the CBF-dependent pathway in radish (Raphanus sativus L.).","authors":"Sen Chen, Liang Xu, Yan Wang, Baozhen Mao, Xiaoli Zhang, Qiyu Song, Feng Cui, Yingbo Ma, Junhui Dong, Kai Wang, Hongyu Bi, Liwang Liu","doi":"10.1186/s43897-024-00135-x","DOIUrl":"10.1186/s43897-024-00135-x","url":null,"abstract":"<p><p>Cold stress adversely affects crop growth and development. Radish is an important root vegetable crop, and its taproot formation is susceptible to low temperatures. However, the molecular basis of the cold stress response has not yet been fully dissected in radish. Here, a sucrose phosphate synthase gene (RsSPS1) was identified through a genome-wide association study and transcriptome analysis. RsSPS1 was responsible for sucrose synthesis, and sucrose was shown to be involved in taproot growth, cambium activity, and cold tolerance in radish. RsSPS1 regulated cambium activity and cold stress response by modulating sucrose content. Moreover, RsWRKY40 was identified as the upstream transcription activator of RsSPS1 by binding to its promoter. RsWRKY40 functioned in cambium activity and cold tolerance by modulating RsSPS1-mediated sucrose accumulation. Furthermore, RsWRKY40 promoted the RsCBF1 and RsCBF2 expression levels, resulting in elevated cold resilience. RsWRKY40 also enhanced its own transcription, forming a positive auto-regulatory loop to regulate cold stress response in radish. Together, a transcription module of RsWRKY40 orchestrated cold stress response by integrating sucrose accumulation and the CBF-dependent pathway was uncovered. These findings would provide novel insight into the molecular mechanism underlying cold-responsive sucrose accumulation and cambium activity and facilitate the genetic improvement of cold tolerance in radish breeding programs.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"14"},"PeriodicalIF":10.6,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chao Gu, Ying Xu, Lei Wu, Xueping Wang, Kaijie Qi, Xin Qiao, Zewen Wang, Qionghou Li, Min He, Shaoling Zhang
{"title":"Long-read genome sequencing reveals the sequence characteristics of pear self-incompatibility locus.","authors":"Chao Gu, Ying Xu, Lei Wu, Xueping Wang, Kaijie Qi, Xin Qiao, Zewen Wang, Qionghou Li, Min He, Shaoling Zhang","doi":"10.1186/s43897-024-00132-0","DOIUrl":"10.1186/s43897-024-00132-0","url":null,"abstract":"<p><p>The S-RNase-based self-incompatibility locus (S-locus) in Petunia species contains 16-20 F-box genes, which collaboratively function in the recognition and subsequent degradation of non-self S-RNases, while distinguishing them from self S-RNase. However, the number of S-locus F-box genes (SFBBs) physically interacted with non-self S-RNases remains uncertain in Pyrus species. Utilizing Pacbio long-read sequencing, we successfully assembled the genome of pear cultivar 'Yali' (Pyrus bretschneideri), and identified 19 SFBBs from the Pyrus S<sub>17</sub>-locus spanning approximately 1.78 Mb. Additionally, we identified 17-21 SFBBs from other Pyrus and Malus S-loci spanning a range of 1.35 to 2.64 Mb. Based on the phylogenetic analysis, it was determined that Pyrus and Malus SFBBs could be classified into 22 groups, denoted as I to XXII. At amino acid level, SFBBs within a given group exhibited average identities ranged from 88.9% to 97.9%. Notably, all 19 SFBBs from the S<sub>17</sub>-locus co-segregated with S<sub>17</sub>-RNase, with 18 of them being specifically expressed in pollen. Consequently, these 18 pollen-specifically expressed SFBBs are considered potential candidates for the pollen-S determinant. Intriguingly, out of the 18 pollen-specifically expressed SFBBs, eight demonstrated interactions with at least one non-self S-RNase, while the remaining SFBBs failed to recognize any S-RNase. These findings provide compelling evidence supporting the existence of a collaborative non-self-recognition system governing self-incompatibility in pear species.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"13"},"PeriodicalIF":10.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daiquan Jiang, Huaibin Lin, Zhenhua Liu, Keke Qi, Wenjin Zhang, Hongyang Wang, Chengcai Zhang, Lu Zhu, Jiaojiao Zhu, Yan Zhang, Luqi Huang, Sheng Wang, Yang Pan, Lanping Guo
{"title":"Polyacetylenes and sesquiterpenes in Chinese traditional herb Atractylodes lancea: biomarkers and synergistic effects in red secretory cavities.","authors":"Daiquan Jiang, Huaibin Lin, Zhenhua Liu, Keke Qi, Wenjin Zhang, Hongyang Wang, Chengcai Zhang, Lu Zhu, Jiaojiao Zhu, Yan Zhang, Luqi Huang, Sheng Wang, Yang Pan, Lanping Guo","doi":"10.1186/s43897-024-00130-2","DOIUrl":"10.1186/s43897-024-00130-2","url":null,"abstract":"","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"11"},"PeriodicalIF":10.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liuzi Zhang, Huihui Tao, Jianting Zhang, Yuyan An, Liangju Wang
{"title":"5-Aminolevulinic acid activates the MdWRKY71-MdMADS1 module to enhance anthocyanin biosynthesis in apple.","authors":"Liuzi Zhang, Huihui Tao, Jianting Zhang, Yuyan An, Liangju Wang","doi":"10.1186/s43897-024-00127-x","DOIUrl":"10.1186/s43897-024-00127-x","url":null,"abstract":"<p><p>5-Aminolevulinic acid (ALA), as a natural plant growth regulator, is well known for promoting red fruit coloring by enhancing anthocyanin accumulation. However, the underlying mechanisms remain elusive. In this study, we firstly demonstrated that ALA upregulates gene expression of the transcription factor MdMADS1, which in turn directly binds to and activates transcription of the key anthocyanin biosynthetic genes, MdCHS and MdUFGT. Then, we identified a novel WRKY transcription factor, MdWRKY71, that interacts with MdMADS1. Through gene manipulation, we revealed that MdWRKY71 plays a pivotal role in ALA-induced anthocyanin accumulation, highlighting its regulatory significance in this process. Further investigation unveiled that MdWRKY71 not only activates MdMADS1 transcription but also enhances its transcriptional activation on its target genes, MdCHS and MdUFGT. Additionally, we discovered that MdWRKY71 independently binds to and activates the transcription of two other anthocyanin biosynthetic genes, MdANS and MdDFR. The protein-protein interaction between MdWRKY71 and MdMADS1 amplifies the transcriptional activation of these genes by MdWRKY71. These findings delineate a fine and complex regulatory framework where MdWRKY71 and MdMADS1 coordinately regulate anthocyanin biosynthesis in apples, providing new insights into the molecular control of fruit coloration and offering potential target genes for breeding aimed at enhancing fruit quality.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"10"},"PeriodicalIF":10.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11789342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus.","authors":"Xiahui Lin, Shaojia Li, Yanna Shi, Yuchen Ma, Yinchun Li, Haohan Tan, Bo Zhang, Changjie Xu, Kunsong Chen","doi":"10.1186/s43897-024-00126-y","DOIUrl":"10.1186/s43897-024-00126-y","url":null,"abstract":"<p><p>Organic acid is a crucial indicator of fruit quality traits. Citric acid, the predominant organic acid in citrus fruit, directly influences its edible quality and economic value. While the transcriptional regulatory mechanisms of citric acid metabolism have been extensively studied, the understanding about the transcriptional and epigenetic co-regulation mechanisms is limited. This study characterized a transcription factor, CitGATA7, which directly binds to and activates the expression of genes associated with the glutamine synthetase pathway regulating citric acid degradation. These genes include the aconitase encoding gene CitACO3, the isocitrate dehydrogenase encoding gene CitIDH1, and the glutamine synthetase encoding gene CitGS1. Furthermore, CitGATA7 physically interacts with the histone acetyltransferase CitHAG28 to enhance histone 3 acetylation levels near the transcription start site of CitACO3, CitIDH1, and CitGS1, thereby increasing their transcription and promoting citric acid degradation. The findings demonstrate that the CitGATA7-CitHAG28 protein complex transcriptionally regulate the expression of the GS pathway genes, i.e., CitACO3, CitIDH1, and CitGS1, via histone acetylation, thus promoting citric acid catabolism. This study establishes a direct link between transcriptional regulation and histone acetylation regarding citric acid metabolism, providing insights for strategies to manipulate organic acid accumulation in fruit.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"8"},"PeriodicalIF":10.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tingting Du, Dong Meng, Hongyan Cao, Yi Lian, Rui Wu, Tengyue Liu, Tianyi Wang, Cai Qin, Zhihua Song, Biying Dong, Yujie Fu, Qing Yang
{"title":"Sorbitol induces flavonoid accumulation as a secondary signal via the nanoencapsulated SPc/lncRNA809-MmNAC17 module against Alternaria alternata in Malus micromalus.","authors":"Tingting Du, Dong Meng, Hongyan Cao, Yi Lian, Rui Wu, Tengyue Liu, Tianyi Wang, Cai Qin, Zhihua Song, Biying Dong, Yujie Fu, Qing Yang","doi":"10.1186/s43897-024-00125-z","DOIUrl":"10.1186/s43897-024-00125-z","url":null,"abstract":"<p><p>Sorbitol is an important primary metabolite that serves as both a carbon source and signal to pathogens. The leaf diseases caused by Alternata alternata are particularly serious in crabapple (Malus micromalus). Here, we found that sorbitol can enhance the resistance of crabapple to A. alternata R1 by increasing the content of flavonoid catechin. Nanomaterials as an emerging technology tool can efficiently deliver lncRNA to target cells. Here, we found nanoencapsulated lncRNA809 (SPc/lncRNA809) exhibits significant resistance to R1strain. To elucidate the effect of SPc/lncRNA809 on flavonoids catechin synthesis, we observed the expression of lncRNA809 was consistent with that of MmNAC17 which regulates the synthesis of catechin and both could jointly respond to sorbitol. MmNAC17 induced the accumulation of catechin in vivo by directly activating the expression of catechin synthase genes MmF3H and MmLAR. Correspondingly, overexpression of lncRNA809 significantly upregulated the expression of MmNAC17 and enhanced the disease resistance. This study reveals for the first time that sorbitol positively regulates the expression of MmNAC17 through lncRNA809, promoting the accumulation of catechin via the expression of MmF3H and MmLAR, ultimately improving the defense response of M. micromalus. This research provides a crucial foundation for the establishment and application of sorbitol-based signaling regulatory networks.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"5"},"PeriodicalIF":10.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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