Molecular Horticulture最新文献_第2页

Development of a low-penetrance mitosis instead of meiosis system in tomato. 番茄低外显率有丝分裂替代减数分裂系统的建立。

IF 8.1

Molecular Horticulture Pub Date : 2026-04-01 DOI: 10.1186/s43897-025-00207-6

Tong Zhao, Yi Zhang, Jiayao Wu, Iqbal Hussain, Weiqiang Li, Fan Ping, Kaiwen Liu, Changtian Pan, Xiaolin Yu

引用次数: 0

The VaATG6-VaBI-1 module coordinates ER-associated autophagy and ROS homeostasis for cold tolerance in grapevine. vatg6 - vabi -1模块在葡萄抗寒性中协调er相关的自噬和ROS稳态。

IF 8.1

Molecular Horticulture Pub Date : 2026-03-10 DOI: 10.1186/s43897-025-00206-7

Qiaoling Zheng, Fang Ma, Chang Liu, Qinhan Yu, Ningbo Zhang, Weirong Xu

{"title":"The VaATG6-VaBI-1 module coordinates ER-associated autophagy and ROS homeostasis for cold tolerance in grapevine.","authors":"Qiaoling Zheng, Fang Ma, Chang Liu, Qinhan Yu, Ningbo Zhang, Weirong Xu","doi":"10.1186/s43897-025-00206-7","DOIUrl":"10.1186/s43897-025-00206-7","url":null,"abstract":"Cold stress is a major constraint on plant productivity, especially in perennial crops like grapevine (Vitis spp.), where it disrupts cellular homeostasis and induces oxidative damage. Here, we identify VaATG6, a key regulator of autophagy, and its interaction with VaBI-1, an ER-localized modulator, as central components of the cold stress response in Vitis amurensis. Cold exposure rapidly induced VaATG6 transcription and triggered its dynamic relocalization from the nucleus to the ER and autophagosomes, enhancing autophagic flux, reducing reactive oxygen species (ROS) accumulation, and improving cold tolerance. In contrast, VaBI-1 acted as a modulator, fine-tuned autophagy and ROS levels through its interaction with VaATG6. Notably, cold stress strengthened the VaATG6-VaBI-1 interaction, linking a coordinated mechanism that integrated autophagy with ER homeostasis. Overexpression of VaATG6 in cold-sensitive V. vinifera enhanced antioxidant defenses and promoted soluble sugar accumulation, significantly improving cold tolerance. Conversely, VaATG6 loss-of-function mutants exhibited impaired autophagy, elevated ROS accumulation, and increased cold sensitivity. These findings establish the VaATG6-VaBI-1 regulatory module as a core mechanism for cold-induced autophagy and oxidative stress adaptation. Our results highlight VaATG6 overexpression and VaBI-1 modulation as promising genetic strategies to enhance cold tolerance in perennial crops.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147391390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into selective signals and local adaptation of a litchi subspecies. 对荔枝亚种的选择信号和局部适应的基因组见解。

IF 8.1

Molecular Horticulture Pub Date : 2026-03-09 DOI: 10.1186/s43897-025-00205-8

Pengfei Wang, Xueren Cao, Hui Zhang, Huanling Li, Huiyun Zhang, Songgang Li, Jiwang Hong, Jian Zheng, Xinping Luo, Chengjie Chen, Lei Zhang, Jiabao Wang

引用次数: 0

Golden leaf formation of Populus nigra is associated with the up-regulation of Stay-Green expression. 黑杨金叶形成与Stay-Green基因表达上调有关。

IF 8.1

Molecular Horticulture Pub Date : 2026-03-06 DOI: 10.1186/s43897-025-00204-9

Wanting Fu, Zimeng Li, Xiaoou Zhai, Haizhen Zhang, Shuang Feng, Aimin Zhou

引用次数: 0

Seizing the key nodes in glabridin biosynthesis network: identification of enzymes for producing licorice hairy roots with high levels of glabridin. 光甘草定生物合成网络关键节点的把握：甘草毛状根高产光甘草定酶的鉴定。

IF 8.1

Molecular Horticulture Pub Date : 2026-03-05 DOI: 10.1186/s43897-025-00202-x

Ningxin Jiang, Xiaoling Ma, Yangxu Wu, Xiaoyi Wei, Yuping Li, Xinyan Guo, Hongxia Wang, Wei Sun, Ling Yuan, Ying Wang, Yongqing Li

{"title":"Seizing the key nodes in glabridin biosynthesis network: identification of enzymes for producing licorice hairy roots with high levels of glabridin.","authors":"Ningxin Jiang, Xiaoling Ma, Yangxu Wu, Xiaoyi Wei, Yuping Li, Xinyan Guo, Hongxia Wang, Wei Sun, Ling Yuan, Ying Wang, Yongqing Li","doi":"10.1186/s43897-025-00202-x","DOIUrl":"10.1186/s43897-025-00202-x","url":null,"abstract":"Glabridin holds significant value in the pharmaceutical and cosmetics industry. Due to the challenges associated with chemical synthesis, Glycyrrhiza glabra (licorice) remains the only source of glabridin. However, its naturally low content is insufficient to meet the increasing market demand. In this study, several inducers of glabridin accumulation-alkali stress, salt-alkali stress, hydroxylamine, 5-azacytidine, and methyl jasmonate (MeJA)-were identified. Using integrated multi-omics analyses and in vitro enzyme assays, two interconnected biosynthetic routes were elucidated: an OMT/ODMT-dependent pathway involving methylation-demethylation cycles and an OMT/ODMT-independent route. Five enzymes, GgIFR (isoflavone reductase), GgTHIS1/2 (7,2',4'-trihydroxyisoflavanol synthases), GgPTS (pterocarpan synthase), GgPTR1/4 (pterocarpan reductases), and GgODMT (O-demethyltransferase), were functionally validated. Using an optimized Agrobacterium rhizogenes-mediated transformation system, we generated transgenic hairy root lines overexpressing these enzymes were generated. GgIFR-overexpressing lines achieved a 44-fold increase in glabridin content (0.507 mg/g DW), comparable to levels in 4-year-old wild roots. This study not only elucidates the complex biosynthetic network of glabridin biosynthesis but also establishes a scalable and sustainable hairy root platform for its industrial production via synthetic biology.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanism of dynamic equilibrium of ascorbate redox status mediated by PbrDHAR5 during scald development in pear fruit. PbrDHAR5介导梨果实烫伤发育过程中抗坏血酸氧化还原状态动态平衡的机制

IF 8.1

Molecular Horticulture Pub Date : 2026-03-04 DOI: 10.1186/s43897-025-00203-w

Xu Zhang, Junpeng Niu, Yanmin Du, Lin Guo, Lichao Chen, Min Ma, Xin Qiao, Weiqi Luo, Chunlu Qian, Guodong Wang, Wenhui Wang, Zhen Zhang, Xinli Geng, Qiuqin Zhang, Lanqing Li, Libin Wang, Shaoling Zhang

{"title":"The mechanism of dynamic equilibrium of ascorbate redox status mediated by PbrDHAR5 during scald development in pear fruit.","authors":"Xu Zhang, Junpeng Niu, Yanmin Du, Lin Guo, Lichao Chen, Min Ma, Xin Qiao, Weiqi Luo, Chunlu Qian, Guodong Wang, Wenhui Wang, Zhen Zhang, Xinli Geng, Qiuqin Zhang, Lanqing Li, Libin Wang, Shaoling Zhang","doi":"10.1186/s43897-025-00203-w","DOIUrl":"10.1186/s43897-025-00203-w","url":null,"abstract":"Ascorbate (AsA) redox status participated in the scald development of Pyrus bretschneideri Rehd. fruit as a cellular redox sensor. By a conjoint analysis of metabolites, enzyme activities and gene expression profiles in AsA-GSH cycle of the chilled pear, PbrDHAR5 was characterized as the candidate gene involved in this process. PbrDHAR5, located in cytosol and nucleus, catalyzed DHA reduction into AsA in vitro and in vivo, elevating AsA redox status and thus fruit chilling tolerance; moreover, the catalytic Cys20 residue in PbrDHAR5 played critical role in this reaction. After analyzing the expression profiles of the differentially expressed TFs, PbrWRKY83 demonstrated higher correlation with PbrDHAR5 than others. PbrWRKY83, located in nucleus, could interact with the only two W-box elements in PbrDHAR5 promoter as monomer and then activate its expression, leading to the improvement of AsA redox status and thus fruit chilling tolerance. In a further study, we explored that the H2O2-mediated S-sulfenylation of Cys20 residue in PbrDHAR5 accumulated upon scald development, suppressed its activity, and thus caused the decrement of AsA redox status. Taken together, our results implied that the H2O2-mediated S-sulfenylation of PbrDHAR5 attenuates the role of PbrWRKY83-PbrDHAR5 module, which positively regulates AsA redox status during scald development in pear.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12958699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147349117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sweet corn metabolic regulatory network spanning the entire life cycle. 甜玉米全生命周期代谢调控网络。

IF 8.1

Molecular Horticulture Pub Date : 2026-03-03 DOI: 10.1186/s43897-025-00201-y

Lili Ma, Jinhua Zuo, Alisdair R Fernie, Shiyu Liu, Xinyuan Zhou, Zixia Jing, Yunxiang Wang, Xinyi Feng, Yiting Ren, Caie Wu, Ronghuan Wang, Yaxing Shi, Yanyan Zheng

引用次数: 0

Fine-tuning RIPENING INHIBITOR (RIN) expression by introducing allelic mutations in its promoter using CRISPR/Cas9 multiplex editing. 利用CRISPR/Cas9多重编辑技术在启动子中引入等位基因突变，微调RIPENING INHIBITOR （RIN）的表达。

IF 8.1

Molecular Horticulture Pub Date : 2026-03-02 DOI: 10.1186/s43897-025-00200-z

Jiaqi Zhou, Chiu-Ling Yang, Diane M Beckles

引用次数: 0

Low temperature-induced cold shock protein modulates determinate growth in cucumber. 低温诱导冷休克蛋白调控黄瓜生长。

IF 8.1

Molecular Horticulture Pub Date : 2026-03-01 DOI: 10.1186/s43897-025-00199-3

Linghao Liu, Haifan Wen, Tiefeng Song, Xiangyu Wang, Junsong Pan, Jian Pan, Tianlai Li

引用次数: 0

RsWRKY15-RsPDR12 module regulates Cd uptake and accumulation by promoting Cd efflux in radish (Raphanus sativus L.). RsWRKY15-RsPDR12模块通过促进萝卜（Raphanus sativus L.） Cd外排调控Cd的吸收和积累。

IF 8.1

Molecular Horticulture Pub Date : 2026-02-12 DOI: 10.1186/s43897-025-00195-7

Shilin Ma, Zhihe Yang, Bingshuang Li, Liang Xu, Yan Wang, Yinbo Ma, Xiaoli Zhang, Jingxue Li, Kai Wang, Xinyu Zhang, Liwang Liu

{"title":"RsWRKY15-RsPDR12 module regulates Cd uptake and accumulation by promoting Cd efflux in radish (Raphanus sativus L.).","authors":"Shilin Ma, Zhihe Yang, Bingshuang Li, Liang Xu, Yan Wang, Yinbo Ma, Xiaoli Zhang, Jingxue Li, Kai Wang, Xinyu Zhang, Liwang Liu","doi":"10.1186/s43897-025-00195-7","DOIUrl":"10.1186/s43897-025-00195-7","url":null,"abstract":"Cadmium (Cd), a non-essential and toxic heavy metal, accumulates in radish taproot and adversely affects radish safety and quality. Pleiotropic drug resistance (PDR) proteins play a crucial role in heavy metal uptake and accumulation in plants. However, the PDR gene-mediated regulation of Cd accumulation remains unexplored in radish. RsPDR12 gene expression significantly increased under Cd stress. RsPDR12 demonstrated Cd transport activity and decreased Cd accumulation in yeast cells, and its overexpression reduced root Cd concentration by Cd2+ efflux in Arabidopsis. RsPDR12 alleviated Cd stress by enhancing membrane permeability and reactive oxygen species (ROS) scavenging in radish plants. RsWRKY15 was identified as the upstream regulatory factor of RsPDR12. Dual-luciferase assay demonstrated that RsWRKY15 bound to the RsPDR12 promoter to activate its transcription. RsWRKY15 expression and promoter activity were significantly induced under Cd stress. RsWRKY15 overexpression mitigated oxidative damage and reduced root Cd concentration in radish and Nicotiana benthamiana plants, respectively. These findings advance understanding of the molecular mechanism underlying the RsWRKY15-RsPDR12 mediated regulatory network of Cd accumulation and support the genetic improvement of low Cd-accumulation cultivars in radish breeding programs.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":"15"},"PeriodicalIF":8.1,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146167099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0