Molecular Horticulture最新文献_第10页

Self S-RNase reduces the expression of two pollen-specific COBRA genes to inhibit pollen tube growth in pear. 自S-RNase降低了两个花粉特异性COBRA基因的表达，抑制了梨花粉管的生长。

Molecular Horticulture Pub Date : 2023-12-01 DOI: 10.1186/s43897-023-00074-z

Lei Wu, Ying Xu, Kaijie Qi, Xueting Jiang, Min He, Yanbo Cui, Jianping Bao, Chao Gu, Shaoling Zhang

{"title":"Self S-RNase reduces the expression of two pollen-specific COBRA genes to inhibit pollen tube growth in pear.","authors":"Lei Wu, Ying Xu, Kaijie Qi, Xueting Jiang, Min He, Yanbo Cui, Jianping Bao, Chao Gu, Shaoling Zhang","doi":"10.1186/s43897-023-00074-z","DOIUrl":"10.1186/s43897-023-00074-z","url":null,"abstract":"Due to self-incompatibility (SI) prevents self-fertilization, natural or artificial cross-pollination has been conducted in many orchards to stabilize fruit yield. However, it is still puzzled which routes of self S-RNase arresting pollen tube growth. Herein, 17 COBRA genes were isolated from pear genome. Of these genes, the pollen-specifically expressed PbCOB.A.1 and PbCOB.A.2 positively mediates pollen tube growth. The promoters of PbCOB.A.1 and/or PbCOB.A.2 were bound and activated by PbABF.E.2 (an ABRE-binding factor) and PbC2H2.K16.2 (a C2H2-type zinc finger protein). Notably, the expressions of PbCOB.A.1, PbCOB.A.2, and PbC2H2.K16.2 were repressed by self S-RNase, suggesting that self S-RNase reduces the expression of PbCOB.A.1 and PbCOB.A.2 by decreasing the expression of their upstream factors, such as PbC2H2.K16.2, to arrest pollen tube growth. PbCOB.A.1 or PbCOB.A.2 accelerates the growth of pollen tubes treated by self S-RNase, but can hardly affect level of reactive oxygen species and deploymerization of actin cytoskeleton in pollen tubes and cannot physically interact with any reported proteins involved in SI. These results indicate that PbCOB.A.1 and PbCOB.A.2 may not relieve S-RNase toxicity in incompatible pollen tube. The information provides a new route to elucidate the arresting pollen tube growth during SI reaction.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"26"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138463014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The CsMYB123 and CsbHLH111 are involved in drought stress-induced anthocyanin biosynthesis in Chaenomeles speciosa. CsMYB123和csmylh111基因参与了干旱胁迫诱导的木瓜花青素合成。

Molecular Horticulture Pub Date : 2023-11-22 DOI: 10.1186/s43897-023-00071-2

Yanshen Ren, Shuangyu Zhang, Qianyi Zhao, Yang Wu, Houhua Li

{"title":"The CsMYB123 and CsbHLH111 are involved in drought stress-induced anthocyanin biosynthesis in Chaenomeles speciosa.","authors":"Yanshen Ren, Shuangyu Zhang, Qianyi Zhao, Yang Wu, Houhua Li","doi":"10.1186/s43897-023-00071-2","DOIUrl":"10.1186/s43897-023-00071-2","url":null,"abstract":"Drought stress has been demonstrated to enhance the biosynthesis of anthocyanins in the leaves, resulting in an increased aesthetic appeal. However, the molecular mechanisms underlying drought-induced anthocyanin biosynthesis in Chaenomeles speciosa remain unclear. In this study, the metabolites of C. speciosa leaves were analyzed, and it was found that the content of cyanidin-3-O-rutinoside increased significantly under drought stress. The differentially expressed genes CsMYB123 and CsbHLH111 were isolated by transcriptomics data analysis and gene cloning, and gene overexpression and VIGS experiments verified that both play important roles in anthocyanin biosynthesis. Subsequently, Y1H and Dual-luciferase reporter assay showed that CsMYB123 binds to the promoters of anthocyanin biosynthesis-related structural genes (such as CsCHI, CsF3H, and CsANS), while CsbHLH111 was shown to bind to the promoter of CsCHI, positively regulating its activity. Furthermore, BIFC and Y2H assays unveiled potential protein-protein interactions between CsMYB123 and CsbHLH111 at the cell nucleus. Collectively, these results shed light on the critical roles played by CsMYB123 and CsbHLH111 in anthocyanin biosynthesis, thus providing a valuable insight into understanding the molecular mechanisms of how the MYB and bHLH genes regulate anthocyanin biosynthesis in the process of leaf coloration in C. speciosa.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10664276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and molecular changes in peach fruit exposed to cold stress conditions. 低温胁迫下桃果的生化和分子变化。

Molecular Horticulture Pub Date : 2023-11-13 DOI: 10.1186/s43897-023-00073-0

Giulia Franzoni, Natasha Damiana Spadafora, Tiziana Maria Sirangelo, Antonio Ferrante, Hilary J Rogers

{"title":"Biochemical and molecular changes in peach fruit exposed to cold stress conditions.","authors":"Giulia Franzoni, Natasha Damiana Spadafora, Tiziana Maria Sirangelo, Antonio Ferrante, Hilary J Rogers","doi":"10.1186/s43897-023-00073-0","DOIUrl":"10.1186/s43897-023-00073-0","url":null,"abstract":"Storage or transportation temperature is very important for preserving the quality of fruit. However, low temperature in sensitive fruit such as peach can induce loss of quality. Fruit exposed to a specific range of temperatures and for a longer period can show chilling injury (CI) symptoms. The susceptibility to CI at low temperature varies among cultivars and genetic backgrounds. Along with agronomic management, appropriate postharvest management can limit quality losses. The importance of correct temperature management during postharvest handling has been widely demonstrated. Nowadays, due to long-distance markets and complex logistics that require multiple actors, the management of storage/transportation conditions is crucial for the quality of products reaching the consumer.Peach fruit exposed to low temperatures activate a suite of physiological, metabolomic, and molecular changes that attempt to counteract the negative effects of chilling stress. In this review an overview of the factors involved, and plant responses is presented and critically discussed. Physiological disorders associated with CI generally only appear after the storage/transportation, hence early detection methods are needed to monitor quality and detect internal changes which will lead to CI development. CI detection tools are assessed: they need to be easy to use, and preferably non-destructive to avoid loss of products.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"24"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiomics analysis provides new insights into the regulatory mechanism of carotenoid biosynthesis in yellow peach peel. 多组学分析为黄桃皮类胡萝卜素生物合成的调控机制提供了新的见解。

Molecular Horticulture Pub Date : 2023-11-03 DOI: 10.1186/s43897-023-00070-3

Jiarui Zheng, Xiaoyan Yang, Jiabao Ye, Dongxue Su, Lina Wang, Yongling Liao, Weiwei Zhang, Qijian Wang, Qiangwen Chen, Feng Xu

{"title":"Multiomics analysis provides new insights into the regulatory mechanism of carotenoid biosynthesis in yellow peach peel.","authors":"Jiarui Zheng, Xiaoyan Yang, Jiabao Ye, Dongxue Su, Lina Wang, Yongling Liao, Weiwei Zhang, Qijian Wang, Qiangwen Chen, Feng Xu","doi":"10.1186/s43897-023-00070-3","DOIUrl":"10.1186/s43897-023-00070-3","url":null,"abstract":"Carotenoids, as natural tetraterpenes, play a pivotal role in the yellow coloration of peaches and contribute to human dietary health. Despite a relatively clear understanding of the carotenoid biosynthesis pathway, the regulatory mechanism of miRNAs involved in carotenoid synthesis in yellow peaches remain poorly elucidated. This study investigated a total of 14 carotenoids and 40 xanthophyll lipids, including six differentially accumulated carotenoids: violaxanthin, neoxanthin, lutein, zeaxanthin, cryptoxanthin, and (E/Z)-phytoene. An integrated analysis of RNA-seq, miRNA-seq and degradome sequencing revealed that miRNAs could modulate structural genes such as PSY2, CRTISO, ZDS1, CHYB, VDE, ZEP, NCED1, NCED3 and the transcription factors NAC, ARF, WRKY, MYB, and bZIP, thereby participating in carotenoid biosynthesis and metabolism. The authenticity of miRNAs and target gene was corroborated through quantitative real-time PCR. Moreover, through weighted gene coexpression network analysis and a phylogenetic evolutionary study, coexpressed genes and MYB transcription factors potentially implicated in carotenoid synthesis were identified. The results of transient expression experiments indicated that mdm-miR858 inhibited the expression of PpMYB9 through targeted cleavage. Building upon these findings, a regulatory network governing miRNA-mediated carotenoid synthesis was proposed. In summary, this study comprehensively identified miRNAs engaged in carotenoid biosynthesis and their putative target genes, thus enhancing the understanding of carotenoid accumulation and regulatory mechanism in yellow peach peel and expanding the gene regulatory network of carotenoid synthesis.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71427420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ABA-AtNAP-SAG113 PP2C module regulates leaf senescence by dephoshorylating SAG114 SnRK3.25 in Arabidopsis. ABA-AtNAP-SAG113PP2C模块通过使拟南芥中的SAG114-SnRK3.25去磷酸化来调节叶片衰老。

Molecular Horticulture Pub Date : 2023-10-30 DOI: 10.1186/s43897-023-00072-1

Gaopeng Wang, Xingwang Liu, Su-Sheng Gan

{"title":"The ABA-AtNAP-SAG113 PP2C module regulates leaf senescence by dephoshorylating SAG114 SnRK3.25 in Arabidopsis.","authors":"Gaopeng Wang, Xingwang Liu, Su-Sheng Gan","doi":"10.1186/s43897-023-00072-1","DOIUrl":"10.1186/s43897-023-00072-1","url":null,"abstract":"We previously reported that ABA inhibits stomatal closure through AtNAP-SAG113 PP2C regulatory module during leaf senescence. The mechanism by which this module exerts its function is unknown. Here we report the identification and functional analysis of SAG114, a direct target of the regulatory module. SAG114 encodes SnRK3.25. Both bimolecular fluorescence complementation (BiFC) and yeast two-hybrid assays show that SAG113 PP2C physically interacts with SAG114 SnRK3.25. Biochemically the SAG113 PP2C dephosphorylates SAG114 in vitro and in planta. RT-PCR and GUS reporter analyses show that SAG114 is specifically expressed in senescing leaves in Arabidopsis. Functionally, the SAG114 knockout mutant plants have a significantly bigger stomatal aperture and a much faster water loss rate in senescing leaves than those of wild type, and display a precocious senescence phenotype. The premature senescence phenotype of sag114 is epistatic to sag113 (that exhibits a remarkable delay in leaf senescence) because the sag113 sag114 double mutant plants show an early leaf senescence phenotype, similar to that of sag114. These results not only demonstrate that the ABA-AtNAP-SAG113 PP2C regulatory module controls leaf longevity by dephosphorylating SAG114 kinase, but also reveal the involvement of the SnRK3 family gene in stomatal movement and water loss during leaf senescence.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71414355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient genome editing in grapevine using CRISPR/LbCas12a system. 利用CRISPR/LbCas12a系统对葡萄基因组进行高效编辑。

IF 10.6

Molecular Horticulture Pub Date : 2023-10-18 DOI: 10.1186/s43897-023-00069-w

Chong Ren, Elias Kirabi Gathunga, Xue Li, Huayang Li, Junhua Kong, Zhanwu Dai, Zhenchang Liang

{"title":"Efficient genome editing in grapevine using CRISPR/LbCas12a system.","authors":"Chong Ren, Elias Kirabi Gathunga, Xue Li, Huayang Li, Junhua Kong, Zhanwu Dai, Zhenchang Liang","doi":"10.1186/s43897-023-00069-w","DOIUrl":"10.1186/s43897-023-00069-w","url":null,"abstract":"Clustered regularly interspaced short palindromic repeats (CRISPR) /Cas12a system, also known as CRISPR/Cpf1, has been successfully harnessed for genome engineering in many plants, but not in grapevine yet. Here we developed and demonstrated the efficacy of CRISPR/Cas12a from Lachnospiraceae bacterium ND2006 (LbCas12a) in inducing targeted mutagenesis by targeting the tonoplastic monosaccharide transporter1 (TMT1) and dihydroflavonol-4-reductase 1 (DFR1) genes in 41B cells. Knockout of DFR1 gene altered flavonoid accumulation in dfr1 mutant cells. Heat treatment (34℃) improved the editing efficiencies of CRISPR/LbCas12a system, and the editing efficiencies of TMT1-crRNA1 and TMT1-crRNA2 increased from 35.3% to 44.6% and 29.9% to 37.3% after heat treatment, respectively. Moreover, the sequences of crRNAs were found to be predominant factor affecting editing efficiencies irrespective of the positions within the crRNA array designed for multiplex genome editing. In addition, genome editing with truncated crRNAs (trucrRNAs) showed that trucrRNAs with 20 nt guide sequences were as effective as original crRNAs with 24 nt guides in generating targeted mutagenesis, whereas trucrRNAs with shorter regions of target complementarity ≤ 18 nt in length may not induce detectable mutations in 41B cells. All these results provide evidence for further applications of CRISPR/LbCas12a system in grapevine as a powerful tool for genome engineering.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"21"},"PeriodicalIF":10.6,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49683033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a molecular marker for cherry crinkle leaf disease. 樱桃皱叶病分子标记的研究进展。

Molecular Horticulture Pub Date : 2023-10-08 DOI: 10.1186/s43897-023-00068-x

Jing Wang, Xiaoming Zhang, Guohua Yan, Yu Zhou, Xuwei Duan, Chuanbao Wu, Xin Zhang, Kaichun Zhang

引用次数: 0

Stress responses of plants through transcriptome plasticity by mRNA alternative polyadenylation. 植物通过信使核糖核酸替代聚腺苷酸化的转录组可塑性的应激反应。

Molecular Horticulture Pub Date : 2023-09-28 DOI: 10.1186/s43897-023-00066-z

Jiawen Zhou, Qingshun Quinn Li

{"title":"Stress responses of plants through transcriptome plasticity by mRNA alternative polyadenylation.","authors":"Jiawen Zhou, Qingshun Quinn Li","doi":"10.1186/s43897-023-00066-z","DOIUrl":"10.1186/s43897-023-00066-z","url":null,"abstract":"The sessile nature of plants confines their responsiveness to changing environmental conditions. Gene expression regulation becomes a paramount mechanism for plants to adjust their physiological and morphological behaviors. Alternative polyadenylation (APA) is known for its capacity to augment transcriptome diversity and plasticity, thereby furnishing an additional set of tools for modulating gene expression. APA has also been demonstrated to exhibit intimate associations with plant stress responses. In this study, we review APA dynamic features and consequences in plants subjected to both biotic and abiotic stresses. These stresses include adverse environmental stresses, and pathogenic attacks, such as cadmium toxicity, high salt, hypoxia, oxidative stress, cold, heat shock, along with bacterial, fungal, and viral infections. We analyzed the overarching research framework employed to elucidate plant APA response and the alignment of polyadenylation site transitions with the modulation of gene expression levels within the ambit of each stress condition. We also proposed a general APA model where transacting factors, including poly(A) factors, epigenetic regulators, RNA m6A modification factors, and phase separation proteins, assume pivotal roles in APA related transcriptome plasticity during stress response in plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"19"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of single-cell multi-omics approaches in horticulture research. 单细胞多组学方法在园艺研究中的应用。

Molecular Horticulture Pub Date : 2023-09-26 DOI: 10.1186/s43897-023-00067-y

Jun Zhang, Mayra Ahmad, Hongbo Gao

引用次数: 0

Small secreted peptides (SSPs) in tomato and their potential roles in drought stress response. 番茄中的小分泌肽及其在干旱胁迫反应中的潜在作用。

Molecular Horticulture Pub Date : 2023-08-25 DOI: 10.1186/s43897-023-00063-2

Kexin Xu, Dongdong Tian, TingJin Wang, Aijun Zhang, Mohamed Abdou Youssef Elsadek, Weihong Liu, Liping Chen, Yongfeng Guo

{"title":"Small secreted peptides (SSPs) in tomato and their potential roles in drought stress response.","authors":"Kexin Xu, Dongdong Tian, TingJin Wang, Aijun Zhang, Mohamed Abdou Youssef Elsadek, Weihong Liu, Liping Chen, Yongfeng Guo","doi":"10.1186/s43897-023-00063-2","DOIUrl":"10.1186/s43897-023-00063-2","url":null,"abstract":"Tomato (Solanum lycopersicum) is one of the most important vegetable crops in the world and abiotic stresses often cause serious problems in tomato production. It is thus important to identify new regulators in stress response and to devise new approaches to promote stress tolerance in tomato. Previous studies have shown that small secreted peptides (SSPs) are important signal molecules regulating plant growth and stress response by mediating intercellular communication. However, little is known about tomato SSPs, especially their roles in responding to abiotic stresses. Here we report the identification of 1,050 putative SSPs in the tomato genome, 557 of which were classified into 38 known SSP families based on their conserved domains. GO and transcriptome analyses revealed that a large proportion of SlSSPs might be involved in abiotic stress response. Further analysis indicated that stress response related cis-elements were present on the SlCEP promotors and a number of SlCEPs were significantly upregulated by drought treatments. Among the drought-inducible SlCEPs, SlCEP10 and SlCEP11b were selected for further analysis via exogenous application of synthetic peptides. The results showed that treatments with both SlCEP10 and SlCEP11b peptides enhanced tomato drought stress tolerance, indicating the potential roles of SlSSPs in abiotic stress response.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"3 1","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10515272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41142657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0