Molecular Horticulture最新文献_第9页

Strawberry cryptochrome FvCRY1 and FvCRY2 transcriptionally regulate anthocyanin biosynthesis and sugar metabolism. 草莓隐色素FvCRY1和FvCRY2转录调控花青素生物合成和糖代谢。

IF 8.1

Molecular Horticulture Pub Date : 2025-12-02 DOI: 10.1186/s43897-025-00197-5

Lianxi Zhang, Anqi Lin, Xiaoyi Bi, Yuxuan Zhu, Lin Ye, Jiwen Lian, Pengbo Xu, Hongli Lian

{"title":"Strawberry cryptochrome FvCRY1 and FvCRY2 transcriptionally regulate anthocyanin biosynthesis and sugar metabolism.","authors":"Lianxi Zhang, Anqi Lin, Xiaoyi Bi, Yuxuan Zhu, Lin Ye, Jiwen Lian, Pengbo Xu, Hongli Lian","doi":"10.1186/s43897-025-00197-5","DOIUrl":"10.1186/s43897-025-00197-5","url":null,"abstract":"Anthocyanin pigmentation and soluble sugar content are important factors affecting the quality of strawberry fruits, and their accumulation processes are profoundly influenced by light signals. However, the molecular mechanism through which light regulates this process remains to be fully elucidated. Here, we identified two blue light receptors, FvCRY1 and FvCRY2, from woodland strawberry. Functional complementation in Arabidopsis cryptochrome mutant confirmed their photoperceptor roles. Stable overexpression of FvCRYs in cultivated strawberry (Fragaria × ananassa 'Ningyu') significantly enhanced anthocyanin accumulation and soluble sugar content, concomitant with upregulation of key biosynthetic genes. Strikingly, yeast one-hybrid and dual-luciferase assays revealed FvCRYs possess intrinsic transcriptional activation domains that directly bind E-box cis-elements in promoters of anthocyanin synthesis gene FvCHS2, FvDFR2, FvCHI, and sugar metabolism-related genes FvSFP9 and FvINV. This DNA-binding capacity is potentiated by blue light, enabling transcriptional expression of anthocyanin synthesis and sugar metabolism genes. Our findings unveil a non-canonical cryptochrome signaling mechanism involving direct receptor-promoter interaction, redefining our understanding of photoreceptor-mediated frui quality regulation.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"63"},"PeriodicalIF":8.1,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12670867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145655956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of major loci governing 13 agronomic traits and the fine-mapping of CaSUN29 regulating fruit length in pepper. 辣椒13个农艺性状的主要位点鉴定及调节果实长度的CaSUN29基因的精细定位。

IF 8.1

Molecular Horticulture Pub Date : 2025-12-01 DOI: 10.1186/s43897-025-00179-7

Yihao Wang, Lingkui Zhang, Xiaolong Yang, Feng Cheng, Bin Chen, Xiaofen Zhang, Sansheng Geng, Heshan Du

{"title":"Identification of major loci governing 13 agronomic traits and the fine-mapping of CaSUN29 regulating fruit length in pepper.","authors":"Yihao Wang, Lingkui Zhang, Xiaolong Yang, Feng Cheng, Bin Chen, Xiaofen Zhang, Sansheng Geng, Heshan Du","doi":"10.1186/s43897-025-00179-7","DOIUrl":"10.1186/s43897-025-00179-7","url":null,"abstract":"Pepper (Capsicum spp.), one of the world's most important vegetable and spice crops, has attracted considerable attention due to its phenotypic diversity, abundant secondary metabolites (particularly capsaicinoids), and substantial economic value. However, current research on the genetic basis and key regulatory genes underlying most traits in pepper is limited. Therefore, in this study, we used two parental lines that presented multiple phenotypic differences-namely, BVRC1 and BVRC25-to generate a recombinant inbred line (RIL) population (F10 generation, 216 lines), after which we performed whole-genome re-sequencing on all lines. Based on a high-resolution bin map, 19 significant loci were identified in association with 13 traits, explaining an average of 26% of the phenotypic variance in the RILs. On the basis of uncovering the major effect locus FL-3.2 (FS-3.1) for fruit shape/fruit length, we constructed new genetic populations to finely map and clone a novel minor-effect regulatory locus FL-10.1 for fruit length. Candidate gene CaSUN29 encoded an IQD protein that was specifically expressed in the early stage of fruit development, affected cell expansion in the pepper pericarp, and subsequently impacted fruit elongation. Virus-induced gene silencing of CaSUN29 in pepper resulted in shorter fruit, while heterologous CaSUN29 overexpression in tomato led to longer fruit. In contrast to the pleiotropic locus FL-3.2, which regulated fruit length, width, and shape simultaneously, FL-10.1 only regulated fruit length. Based on the identified loci, a trait-locus network was constructed to understand the correlations among traits based on the degree of locus linkage. These findings are helpful for our exploration of new genes regulating important traits, providing better understanding of the linkage relationships among complex traits.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"64"},"PeriodicalIF":8.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding disease resistance: engineered NLRS for broad-spectrum protection. 扩大抗病性：用于广谱保护的工程NLRS。

IF 8.1

Molecular Horticulture Pub Date : 2025-11-24 DOI: 10.1186/s43897-025-00217-4

Tingting Zhou, Jiajun Wang, Yunjing Wang

引用次数: 0

Correction: TILLCANN: a TILLING platform in Cannabis sativa for mutation discovery and crop improvement. 更正：TILLCANN：用于突变发现和作物改良的大麻耕作平台。

IF 8.1

Molecular Horticulture Pub Date : 2025-11-14 DOI: 10.1186/s43897-025-00221-8

Diana Duarte-Delgado, Konstantinos G Alexiou, Marta Pujol, Cristobal Uauy, Nikolai M Adamski, Victoria Vidal, Anthony Torres, Christopher Zalewski, Reginald Gaudino, Amparo Monfort, Jason Argyris

引用次数: 0

A chromosome-level genome assembly of Alpinia officinarum Hance sheds new light on its evolution and flavonoid biosynthesis. 一种染色体水平的Alpinia officinarum Hance基因组组装为其进化和类黄酮生物合成提供了新的线索。

IF 8.1

Molecular Horticulture Pub Date : 2025-11-04 DOI: 10.1186/s43897-025-00191-x

Hongyang Gao, Hongli Shang, Xi Huang, Ziqi Zheng, Haoran Yu, Quan Yang

{"title":"A chromosome-level genome assembly of Alpinia officinarum Hance sheds new light on its evolution and flavonoid biosynthesis.","authors":"Hongyang Gao, Hongli Shang, Xi Huang, Ziqi Zheng, Haoran Yu, Quan Yang","doi":"10.1186/s43897-025-00191-x","DOIUrl":"10.1186/s43897-025-00191-x","url":null,"abstract":"Alpinia officinarum Hance, a medicinal and edible plant in the Zingiberaceae family, has applications in pharmaceuticals, food, nutraceuticals, spices, cosmetics, and fruit and vegetable preservation. However, the molecular mechanisms governing the biosynthesis of flavonoids, the primary bioactive compounds in A. officinarum, remain unclear. This study provided the first chromosome-grade genome assembly of A. officinarum, revealing it to be a triploid species with a 2.10-Gb haploid genome. Using integrated transcriptomic and metabolomic analysis, 107 flavonoid compounds selectively accumulated in the rhizome and prospective rhizome-specific flavonoid genes were identified. Gene-metabolite correlation analysis and weighted gene co-expression network analysis suggested that AobHLH94 was imperative to flavonoid biosynthesis regulation in the rhizome. Functional assays confirmed that AobHLH94 was a key regulator in the flavonoid biosynthesis pathway in A. officinarum and enhanced flavonoid accumulation in rice (Oryza sativa). We also discovered that AobHLH94 bound to the e-box elements (-CANNTG-) in the promoters of AoC4H and AoCHI genes, thereby upregulating their expression levels and enhancing flavonoid synthesis. Simultaneously, AobHLH94 indirectly modulated AoCHS. Our research explains the regulatory processes behind rhizome-specific flavonoid accumulation and provides relevant information for future A. officinarum improvement and breeding efforts.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"59"},"PeriodicalIF":8.1,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12584514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145439082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient genome editing in Pseudomonas syringae pv. actinidiae using the CRISPR/FnCas12a system. 丁香假单胞菌的高效基因组编辑。利用CRISPR/FnCas12a系统。

IF 1

Molecular Horticulture Pub Date : 2025-11-03 DOI: 10.1186/s43897-025-00180-0

Zhenzhen Gou, Yue Wang, Chunyi Qin, Fang Yan, Xiangning Du, Zhengyin Xu, Bo Zhu, Pu Liu, Huanbin Zhou, Gongyou Chen

{"title":"Efficient genome editing in Pseudomonas syringae pv. actinidiae using the CRISPR/FnCas12a system.","authors":"Zhenzhen Gou, Yue Wang, Chunyi Qin, Fang Yan, Xiangning Du, Zhengyin Xu, Bo Zhu, Pu Liu, Huanbin Zhou, Gongyou Chen","doi":"10.1186/s43897-025-00180-0","DOIUrl":"10.1186/s43897-025-00180-0","url":null,"abstract":"CRISPR-based gene editing has rarely been studied in plant pathogens. In this report, the CRISPR/FnCas12a system was successfully established for gene editing in Pseudomonas syringae pv. actinidiae (Psa), which causes bacterial canker (BC) of kiwifruit. The system was constructed in a Psa-suitable vector pBBR1-MCS2 to edit hopH1 or/and hopZ5, which encode effectors only present in Psa biovar 3 (Psa3) responsible for BC pandemic in kiwifruit worldwide. Two different CRISPR RNAs (crRNA) were designed to edit either hopH1 or/and hopZ5, and two different sets of PCR primers were used to screen deletions of the target genes and the presence of the vector in Psa. The deletion in Psa was impacted by the position of the DNAs targeted by the crRNAs. The vector-eliminated mutant could receive the editing system iteratively. Interestingly, the double mutant ΔhopZ5ΔhopH1 showed significantly stronger virulence than the wild-type Psa on Actinidia eriantha cv. White (resistant to BC), but weak virulence on A. chinensis cv. Hongyang (highly susceptible to BC), respectively, suggesting that hopH1 or/and hopZ5 potentially matches an unknown resistance gene in White. In summary, we have established the CRISPR/FnCas12a genome-editing system to probe gene function in the pathogen and to explore effector-target interactions in kiwifruit-Psa-pathosyetem.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"60"},"PeriodicalIF":10.0,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12581509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145432446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malate metabolism in horticultural crops: mechanistic insights and agricultural practices for quality improvement. 园艺作物中的苹果酸代谢：质量改进的机理和农业实践。

IF 8.1

Molecular Horticulture Pub Date : 2025-10-23 DOI: 10.1186/s43897-025-00196-6

Ting-Ting Zhao, Lian-Da Du, Chu-Kun Wang, Meng-Meng Wei, Da-Gang Hu

{"title":"Malate metabolism in horticultural crops: mechanistic insights and agricultural practices for quality improvement.","authors":"Ting-Ting Zhao, Lian-Da Du, Chu-Kun Wang, Meng-Meng Wei, Da-Gang Hu","doi":"10.1186/s43897-025-00196-6","DOIUrl":"10.1186/s43897-025-00196-6","url":null,"abstract":"Malate metabolism bridges plant evolutionary adaptation and fruit quality regulation, serving dual roles in energy metabolism (tricarboxylic acid cycle/glycolysis) and environmental stress responses (stomatal control, pH balance). In horticulture, apple malate content dictates flavor profiles, driving divergent consumer preferences (high-sugar in Asia vs. tartness in the West), necessitating precision breeding targeting vacuolar accumulation mechanisms. Recent bioinformatic studies and transporter biology (e.g., Ma1, ALMT) have revealed genetic regulators of malate homeostasis, yet transcriptional regulation and post-translational modifications (PTMs) of transporters remain poorly understood. Notably, cultivated varieties exhibit distinct malate-related traits compared to their wild relatives, a divergence attributable to artificial selection during domestication. Additionally, agroecological factors including light, temperature, and soil conditions, dynamically regulate malate biosynthesis and storage. This metabolic plasticity reflects evolutionary adaptations influenced by domestication. This review integrates molecular physiology and domestication genetics to dissect cross-scale regulation of malate networks. We propose a transporter-engineering framework for developing market-tailored varieties and highlight unresolved questions, including PTM-mediated transporter regulation and metabolic plasticity modeling for climate-resilient crops. Bridging evolutionary adaptation with quality-driven breeding targeting malate, this synthesis advances strategies for sustainable horticulture in shifting agroclimatic landscapes.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"58"},"PeriodicalIF":8.1,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12548192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145348914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: The ILR3-NRTs/NIA1/SWEET12 module regulates nitrogen uptake and utilization in apple. 更正：ilr3 - nrt /NIA1/SWEET12模块调节苹果的氮素吸收和利用。

IF 1

Molecular Horticulture Pub Date : 2025-10-22 DOI: 10.1186/s43897-025-00198-4

Hong-Liang Li, Ran-Xin Liu, Xiang Wu, Xin-Long Guo, Shan-Shan Li, Tian-Tian Wang, Yan-Yan Guo, Xiao-Fei Wang, Chun-Xiang You

引用次数: 0

TILLCANN: a TILLING platform in Cannabis sativa for mutation discovery and crop improvement. tilcann：用于突变发现和作物改良的大麻耕作平台。

IF 8.1

Molecular Horticulture Pub Date : 2025-10-13 DOI: 10.1186/s43897-025-00176-w

Diana Duarte-Delgado, Konstantinos G Alexiou, Marta Pujol, Cristobal Uauy, Nikolai M Adamski, Victoria Vidal, Anthony Torres, Christopher Zalewski, Reginald Gaudino, Amparo Monfort, Jason Argyris

引用次数: 0

PbrNAC34a- PbrMYB3/65- PbrACO2 cascade plays a role in citrate difference between the pericarp and cortex tissues of pear (P. bretschneideri Rehd.) fruit. PbrNAC34a- PbrMYB3/65- pbrnaco2级联在梨果皮和果皮组织柠檬酸盐差异中起作用。

IF 8.1

Molecular Horticulture Pub Date : 2025-10-10 DOI: 10.1186/s43897-025-00177-9

Xu Zhang, Luting Jia, Suling Zhang, Lijuan Zhu, Weilin Wei, Bing Yang, Weiqi Luo, Savithri U Nambeesan, Xin Qiao, Li Jiang, Christopher Ference, Min Ma, Libin Wang, Shaoling Zhang

{"title":"PbrNAC34a- PbrMYB3/65- PbrACO2 cascade plays a role in citrate difference between the pericarp and cortex tissues of pear (P. bretschneideri Rehd.) fruit.","authors":"Xu Zhang, Luting Jia, Suling Zhang, Lijuan Zhu, Weilin Wei, Bing Yang, Weiqi Luo, Savithri U Nambeesan, Xin Qiao, Li Jiang, Christopher Ference, Min Ma, Libin Wang, Shaoling Zhang","doi":"10.1186/s43897-025-00177-9","DOIUrl":"10.1186/s43897-025-00177-9","url":null,"abstract":"Citrate is critical to the flavor of horticultural fruit and governed by ACO. However, the specific ACO and its upstream regulators involved in citrate metabolism during pear (Pyrus spp.) fruit development remained uncharacterized. This study identified and characterized six PbrACOs from the Pyrus bretschneideri Rehd. genome. Comprehensive analyses of citrate levels, cyt/mitACO activities, and PbrACOs expression profiles in the pericarp and cortex tissues of developing 'Yali' and 'Dangshansuli' fruits revealed PbrACO2 as a candidate gene. Subsequently, PbrACO2 was confirmed as a mitochondrial aconitase catalyzing citrate-to-isocitrate conversion in vitro and in vivo. Analysis of differentially expressed transcription factors (TFs) and cis-acting elements in the PbrACO2 promoter identified nuclear PbrMYB3 and PbrMYB65, derived from whole genome duplication/segmental duplication, as candidate upstream regulators. These MYB TFs, without direct relationship, bound, as monomers, to the same two MYB-binding sites in the PbrACO2 promoter to activate its transcription, thereby promoting citrate isomerization in pear and tomato. Further investigation revealed that PbrMYB3 and PbrMYB65 are transcriptionally regulated by PbrNAC34a. Given their tissue-dependent expression profiles, the PbrNAC34a-PbrMYB3/65-PbrACO2 cascade partially accounts for citrate differences between pear fruit pericarp and cortex tissues. These findings enhance understanding of citrate accumulation in Rosaceae fruit and provide genetic resources for pear breeding.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"55"},"PeriodicalIF":8.1,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0