ACS Measurement Science Au最新文献

{"title":"High-Throughput Measurement of Biodegradation Rates Using the Clear-Zone Assay","authors":"Sarah H. M. Av-Ron,&nbsp;Natalie D. Mamrol,&nbsp;Dylan J. Walsh,&nbsp;Katharina A. Fransen,&nbsp;Bradley D. Olsen","doi":"10.1021/acsmeasuresciau.6c00059","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00059","url":null,"abstract":"<p>Plastic pollution has become a major concern, drivingthe searchfor biodegradable-polymer alternatives. Existing standards for measuringbiodegradation are often costly, are time-consuming, and lack reproducibilitybetween different laboratories. Previous research demonstrated thedevelopment of a new cost- and time-efficient biodegradation testbased on the clear-zone assay, enabling polymers to be rapidly classifiedas biodegradable or nonbiodegradable. This work builds upon that assay,addressing challenges in improving automation, signal-to-noise, andanalytical methods to enable a more quantitative comparison betweenmaterials and meaningful extraction of biodegradation rates. The samplepreparation process was modified to ensure uniform particle size distribution,and changes in sample preparation, data processing, and analysis improvedoutput data accuracy. The design of a fully automated biodegradationmonitoring system that runs inside an incubator increased the reproducibilityand reliability of data acquisition. These modifications enhance thehigh-throughput biodegradation assay, enabling an improved accuracy.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1069–1080"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Snapshot Redox Cycling Voltammetry (Snapshot RCV): Fast Generation-Collection Detection across a Wide Potential Range at a Chip-Based Microelectrode Array","authors":"Blake A. Sterling,&nbsp;Miguel Á. Ábrego Tello,&nbsp;Ingrid Fritsch","doi":"10.1021/acsmeasuresciau.6c00091","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00091","url":null,"abstract":"<p>During generation-collection, redox-active species oxidize(orreduce) at generator electrodes and electrochemically convert backat neighboring collector electrodes, yielding dual-current signalsshaped by mass transport, reaction kinetics, and scaled by analyteconcentration. As electrode spacing decreases, diffusion layers overlap,recycling species in a feedback loop (redox cycling, RC) and amplifyingobserved currents. While advantageous, conventional RC techniquesexperience challenges: chronoamperometry (CA) offers high temporalresolution but lacks thermodynamic insight, while cyclic voltammetry(CV) captures the potential window but is constrained by scan durationand background current at high speeds. Here, snapshot redox cyclingvoltammetry (Snapshot RCV) is introduced, which constructs the profileof a conventional RC voltammogram without a potential sweep. By simultaneouslybiasing individual generator electrodes in a microband array to differentvoltages across the potential range while individually holding collectorelectrodes at a reversing potential, high-resolution “spatial”voltammograms are obtained in as little as 1 s, providing steady-stategeneration-collection profiles on a chronoamperometric time scale.This method was evaluated using 0.50 mM Ru(NH₃)₆Cl₃ in 0.10 M KCl on a seven-electrode gold microbandarray (<em>width</em> = 4.08 μm, <em>gap</em> = 4.14 μm, <em>length</em> = 97.17 μm). SnapshotRCV was then performed on other analytes (dopamine (DA), 3,4-dihydroxyphenylalanine(L-DOPA), 3,4-dihydroxyphenylacetic acid (DOPAC), and ascorbic acid(AA)) with different electrochemical properties: standard reductionpotentials (E°), electron transfer rate constants (k°),and following chemical reaction rates. Snapshot RCV captures the voltammetricshape with a 10%–60% increase in collection efficiency overConventional RCV and can isolate inner array electrochemistry fromedge contributions, allowing for postexperimental geometric filtering.The different collection efficiencies and voltammetric shapes fromredox-active molecules, driven by their thermodynamics, kinetics,and chemical mechanisms (EC, ECC′), could make Snapshot RCVa promising technique for qualitative and quantitative analysis whileenabling rapid sensing that is also adaptable to confined spaces.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1223–1238"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversible Microfluidic Platform for Spheroid Culturing, Downstream Characterization, and Dynamic Anticancer Susceptibility Testing","authors":"Iris Renata Sousa Ribeiro,&nbsp;Pedro Henrique Nunes da Silva,&nbsp;Bruna Gabrielle Olsen,&nbsp;Katarina da Silva Vilarins,&nbsp;Eloisa Maria Amador Souza,&nbsp;Isabela Aparecida de Araujo Andreotti,&nbsp;Leandro Ramos Souza Barbosa,&nbsp;Emanuel Carrilho,&nbsp;Angelo Luiz Gobbi,&nbsp;Diego Stefani Teodoro Martinez,&nbsp;Renato Sousa Lima","doi":"10.1021/acsmeasuresciau.6c00140","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00140","url":null,"abstract":"<p>Despite the potentialitiesof three-dimensional (3D)spheroidsto improve the predictive capability of preclinical <em>in vitro</em> assays, their translation into microfluidic drug screening pipelinesremains limited by insufficient operational standardization, restrictedaccess to downstream analyses, and static exposure regimes that inadequatelycapture <em>in vivo</em> mass transport. To address thesedownsides, we here introduce a reversibly bonded polydimethylsiloxanemicrofluidic platform that provides highly reproducible microwell-enabledspheroid generation, nondestructive spheroid retrieval for advancedoff-chip characterization, and dynamic perfusion-based drug-susceptibilitytesting. A standardized operational workflow addressing practicalaspects of microfluidic 3D culture (i.e., bubble suppression, surfacepassivation, and controlled media handling) is delivered, ultimatelyenabling reliable, user-friendly spheroid formation into daily practiceacross distinct biological systems. To date, the system has been successfullyinterrogated for the generation of spheroids derived from breast tumorand zebrafish liver cells. Importantly, as-produced spheroids couldbe retrieved from the reversible microfluidic device for downstreamanalyses, as demonstrated by transmission electron microscopy (TEM).TEM analysis of retrieved spheroid sections revealed preserved cellularultrastructure, including well-defined nuclei and organelles. Thisdata highlights the suitability of the microfluidic platform for downstreamhigh-resolution characterization of as-cultured spheroids. Finally,doxorubicin susceptibility experiments under physiologically relevantperfusion conditions revealed enhanced cytotoxicity compared withstatic conditions, highlighting the critical role of flow-driven masstransport in generating more predictive drug-response profiles. Collectively,this work yields a scalable, reproducible, and accessible microfluidicframework that bridges spheroid culturing, downstream characterization,and dynamic pharmacology, expanding the analytical potential of <em>in vitro</em> microphysiological models for susceptibility testing.These assays can be broadly leveraged beyond drug discovery, includingapplications in toxicity testing of (bio)chemicals and (nano)materialstoward new approach methodologies, safety research, and environmentalassessments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1339–1349"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luminescence Lifetime Imaging: Key Concepts and Advances in the Image Acquisition, Processing, and Analysis Pipeline","authors":"Rishi Harkhoe,&nbsp;Gabriele Ferrari,&nbsp;Ruslan I. Dmitriev,&nbsp;Michael Kühl","doi":"10.1021/acsmeasuresciau.5c00209","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00209","url":null,"abstract":"<p>There is rapidlygrowing interest in applying luminescence lifetimeimaging, i.e., FLIM and PLIM (fluorescence and phosphorescence lifetimeimaging microscopy, respectively), across natural and life sciences.This includes developing new chemistries for luminescence lifetimeread out, new instrumentation and data analysis approaches, as wellas diverse applications in chemical, environmental and life sciences.The methodology captures the luminescence lifetime, an intrinsic fingerprintthat is sensitive to the local physicochemical environment and specificmolecular interactions, making it a valuable quantitative imagingtechnique for tissue characterization, live-cell metabolic studies,materials science studies, and more. Luminescence lifetime imagingis both robust and reproducible when properly executed but demandscareful experimental design and substantial user expertise in termsof choice of imaging modality, instrumentation parameters, image acquisitionsettings and subsequent image processing and analysis. Therefore,this review addresses the principal determinants of imaging data andreviews practical methods and state-of-the-art advances to addressthem. Concurrently, this provides a foundation to discuss and highlightthe current frontiers and future directions of this important imagingmodality.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"873–915"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen Isotope and Water Content Analysis of Ultralow Volume Volcanic Glass","authors":"Zhiqiang Yu,&nbsp;Jialong Hao","doi":"10.1021/acsmeasuresciau.6c00092","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00092","url":null,"abstract":"<p>Hydrogen isotopeand H₂O content of hydratedvolcanicglass are useful proxies for paleoelevation and paleoclimate reconstruction.However, their analysis remains challenging because conventional bulkmethods typically require relatively large amounts (∼10^–3–10^–2 g) of purified andfresh volcanic glass. Volcanic glass from the Mesozoic sediments andscientific drilling core samples is commonly affected by alterationand grain-scale heterogeneity, and the available sample mass is ofteninsufficient for bulk analysis. Here, we present an ultralow sampleconsumption (∼10^–9 g) in situ method fordetermining H₂O contents and hydrogen isotope of hydratedvolcanic glass from Early Cretaceous tuffs. This approach extendssuch analyses to older and less-well-preserved materials and providesa practical basis for high-resolution paleoenvironmental reconstruction.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"923–928"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-resolution Molecular Fingerprinting of Algal Polysaccharides Extracted at Alkaline Hydrothermal Conditions","authors":"Anna Trubetskaya,&nbsp;Luis Leimbrock,&nbsp;Volker Herdegen,&nbsp;Erica Brendler,&nbsp;Carla Vogt,&nbsp;Jan Zuber","doi":"10.1021/acsmeasuresciau.6c00120","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00120","url":null,"abstract":"<p>Alkaline hydrothermalwater extraction was applied to macroalgae <em>Ascophyllum nodosum</em> (<em>A. nodosum</em>) and microalgae <em>Arthrospira platensis</em> (<em>A. platensis</em>) to separate alginate-likepolysaccharides and evaluate their structural integrity. A multidimensionalanalytical workflow combining solution-state ¹H NMR, solid-state ¹³C CP/MAS NMR, and ultrahigh-resolution FT-ICR-MS (ESI andGALDI) was established to bridge polymer backbone analysis with molecular-levelfingerprinting. NMR analyses indicated the retention of characteristicglycosidic and carboxyl-associated functionalities in <em>A. nodosum</em>, whereas corresponding signals were absentin <em>A. platensis</em>, indicating fundamentalcompositional differences. FT-ICR-MS resolved up to 1591 molecularformulas, revealing high extract complexity dominated by lipid-likeand hydrocarbon species, while carbohydrate-assigned formulas werecomparatively underrepresented due to limited ionization efficiency.Nonetheless, the presence of oxygen-rich compounds in the macroalgalresidue sample supported the presence of alginate-related molecules.The combined approach demonstrates the complementary strengths ofNMR and FT-ICR-MS for process monitoring, structural verification,and molecular fingerprinting of complex algal extracts, advancinganalytical strategies for sustainable polysaccharide valorization.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1301–1314"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When Spectroscopies Speak the Same Language: Unifying Rheology, Electrochemical Impedance, and Dielectrics","authors":"Shivangi Mittal,&nbsp;Sachin Shanbhag,&nbsp;Yogesh M. Joshi","doi":"10.1021/acsmeasuresciau.6c00098","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00098","url":null,"abstract":"<p>Spectroscopic techniques measure the dynamical responseof physicalsystems subjected to oscillatory perturbations. For small perturbationsaround the equilibrium state, these spectroscopic methods are unifiedby the common mathematical framework of linear response theory. Thiswork presents a unified perspective on rheological or mechanical spectroscopy,electrochemical impedance spectroscopy, and broadband dielectric spectroscopythrough the lens of linear response theory. Subtle conceptual similaritiesand differences among these techniques are highlighted by analyzingtheir mapping to the linear response theory, basic building blocks,elementary models, and time and frequency domain response functions.Data validation and analysis approaches, including Kramers–Kronigrelations, equivalent circuits, and multimode models are discussed.The shared fundamentals of different spectroscopies enable seamlessexchange of ideas across domains.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"857–872"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Single-Enzyme Electrochemical Biosensor for Lactose Detection in Human Breastmilk and Dairy Products","authors":"Raquel Sánchez-Salcedo,&nbsp;Donna T. Geddes,&nbsp;Nicolas H. Voelcker","doi":"10.1021/acsmeasuresciau.5c00207","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00207","url":null,"abstract":"<p>Lactose, a disaccharidefound predominantly in humanbreast milkand dairy products, plays a vital role in human health and nutrition.While lactose is an essential energy source particularly for infantgrowth, it is also a biomarker of low milk production in breastfeedingwomen. Furthermore, lactose intolerance affects a substantial portionof the global population, impacting the quality of life. Therefore,the ability to detect and quantify lactose is important as a biomarkerfor both milk production and nutritional content. Here, we reporton the development of a single-enzyme electrochemical biosensor forlactose employing lactose oxidase (LOx), which converts lactose tolactobionic acid and hydrogen peroxide. The sensor showed high specificity,achieving a limit of detection of 1.4 μM. The analytical performanceof the sensor was verified in human breast milk as well as bovinemilk. This approach allows for a rapid, reliable, and potentiallyportable platform for lactose detection, making it ideal for bothclinical applications and routine controls in the food industry.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"949–956"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Color-Changing Hydrogels for Visual pH Monitoring of Confined Living Systems","authors":"Wei-Ting Chang,&nbsp;Matthew M. Hausladen,&nbsp;Olivia J. Armendarez,&nbsp;Jenevieve Kuang,&nbsp;Neel S. Joshi,&nbsp;Daniel J. Wilson,&nbsp;Leila F. Deravi","doi":"10.1021/acsmeasuresciau.6c00085","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00085","url":null,"abstract":"<p>Maintaining pH homeostasis is essential for cell viability,yetconfined culture systems such as bioreactors are susceptible to pHdrift, even under controlled nutrient supply. We developed a noninvasivemethod for continuous pH monitoring of microbial cultures using dual-dyecolorimetric sensors covalently bound to pH-responsive hydrogels.A 3D-printed dual-chamber device spatially separates the sensors fromactive cultures, allowing only proton and metabolite diffusion acrossthe semipermeable membrane. This eliminates the risk of sensor obscurationfrom cell turbidity. Quantitative pH measurements were obtained throughimage-based color analysis and numerical processing of the hydrogelresponse, resolving pH within ±0.1 units of those performed usingconventional sensors. Our material strategy offers an alternativeapproach for in situ monitoring of confined culture environments,enabling live tracking of pH dynamics in biological systems.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1206–1215"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative MALDI-MSI Workflow for Lipids with a LC-MS-Based Quality Control Pipeline","authors":"Felix-Levin Hormann,&nbsp;Sven Heiles","doi":"10.1021/acsmeasuresciau.6c00083","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00083","url":null,"abstract":"<p>Mass spectrometry imaging (MSI) has established itselfas a majoranalytical method in the spatial analysis of biological tissue. Besidesqualitative mapping of analyte distributions, recent developmentsaim to achieve quantification of target analytes. After successfulspatial quantification of pharmaceuticals, the field is now shiftingtoward endogenous biomolecules such as lipids and metabolites. Here,we present a quantitative MSI (qMSI) workflow for endogenous lipidsemploying matrix-assisted laser desorption/ionization (MALDI) andstable isotope labeled standards (SILS) integrated into a qualitycontrol pipeline utilizing quantitative reversed-phase liquid chromatographytandem mass spectrometry (RP-LC-MS/MS). We thoroughly characterizedthe standard deposition of 13 lipid SILS with a focus on homogeneityand detection errors on mouse heart septa with intraday coefficientof variations (CVs) of ≤15%. Unlike RP-LC-MS/MS for which CVvalues increase mainly for lowly abundant lipids, variations of lipidsin MALDI-MSI are affected by concentration as well as lipid class.Additionally, we compared lipid annotations based on MALDI-MSI andRP-LC-MS/MS and correlated these annotations between the methods basedon quantification results. This method comparison revealed that mostpolar lipids such as PCs or SMs are captured in both MALDI-MSI andRP-LC-MS/MS, whereas less polar compounds such as TGs exhibit limitedoverlap. These results highlight that a determination of the SILSamounts and lipid annotations via RP-LC-MS/MS is necessary to counterthe increase variability during quantitative MALDI-MSI. To demonstratethe analytical capabilities of the quantitative MALDI-MSI pipelinecombined with RP-LC-MS/MS quality control steps, we analyzed mousekidney from a two-hit heart failure with preserved ejection fractionmodel and achieved good comparability between RP-LC-MS/MS and MALDI-MSIquantitation results. Our data suggest increased ether lipid concentrationsin obese mice kidney, likely to cope with increased oxidative stressinduced by the progressing disease state of the mice.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1194–1205"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}