ACS Measurement Science Au最新文献_第2页

Closing the Knowledge Gap of Post-Acquisition Sample Normalization in Untargeted Metabolomics. 缩小非靶向代谢组学中采集后样本归一化的知识差距。

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-14 eCollection Date: 2024-12-18 DOI: 10.1021/acsmeasuresciau.4c00047

Brian Low, Yukai Wang, Tingting Zhao, Huaxu Yu, Tao Huan

{"title":"Closing the Knowledge Gap of Post-Acquisition Sample Normalization in Untargeted Metabolomics.","authors":"Brian Low, Yukai Wang, Tingting Zhao, Huaxu Yu, Tao Huan","doi":"10.1021/acsmeasuresciau.4c00047","DOIUrl":"10.1021/acsmeasuresciau.4c00047","url":null,"abstract":"Sample normalization is a crucial step in metabolomics for fair quantitative comparisons. It aims to minimize sample-to-sample variations due to differences in the total metabolite amount. When samples lack a specific metabolic quantity to accurately represent their total metabolite amounts, post-acquisition sample normalization becomes essential. Despite many proposed normalization algorithms, understanding remains limited of their differences, hindering the selection of the most suitable one for a given metabolomics study. This study bridges this knowledge gap by employing data simulation, experimental simulation, and real experiments to elucidate the differences in the mechanism and performance among common post-acquisition sample normalization methods. Using public datasets, we first demonstrated the dramatic discrepancies between the outcomes of different sample normalization methods. Then, we benchmarked six normalization methods: sum, median, probabilistic quotient normalization (PQN), maximal density fold change (MDFC), quantile, and class-specific quantile. Our results show that most normalization methods are biased when there is unbalanced data, a phenomenon where the percentages of up- and downregulated metabolites are unequal. Notably, unbalanced data can be sourced from the underlying biological differences, experimental perturbations, and metabolic interference. Beyond normalization algorithms and data structure, our study also emphasizes the importance of considering additional factors contributed by data quality, such as background noise, signal saturation, and missingness. Based on these findings, we propose an evidence-based normalization strategy to maximize sample normalization outcomes, providing a robust bioinformatic solution for advancing metabolomics research with a fair quantitative comparison.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"702-711"},"PeriodicalIF":4.6,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Closing the Knowledge Gap of Post-Acquisition Sample Normalization in Untargeted Metabolomics 缩小非靶向代谢组学中采集后样本归一化的知识差距

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-13 DOI: 10.1021/acsmeasuresciau.4c0004710.1021/acsmeasuresciau.4c00047

Brian Low, Yukai Wang, Tingting Zhao, Huaxu Yu and Tao Huan*,

{"title":"Closing the Knowledge Gap of Post-Acquisition Sample Normalization in Untargeted Metabolomics","authors":"Brian Low, Yukai Wang, Tingting Zhao, Huaxu Yu and Tao Huan*, ","doi":"10.1021/acsmeasuresciau.4c0004710.1021/acsmeasuresciau.4c00047","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00047https://doi.org/10.1021/acsmeasuresciau.4c00047","url":null,"abstract":"Sample normalization is a crucial step in metabolomics for fair quantitative comparisons. It aims to minimize sample-to-sample variations due to differences in the total metabolite amount. When samples lack a specific metabolic quantity to accurately represent their total metabolite amounts, post-acquisition sample normalization becomes essential. Despite many proposed normalization algorithms, understanding remains limited of their differences, hindering the selection of the most suitable one for a given metabolomics study. This study bridges this knowledge gap by employing data simulation, experimental simulation, and real experiments to elucidate the differences in the mechanism and performance among common post-acquisition sample normalization methods. Using public datasets, we first demonstrated the dramatic discrepancies between the outcomes of different sample normalization methods. Then, we benchmarked six normalization methods: sum, median, probabilistic quotient normalization (PQN), maximal density fold change (MDFC), quantile, and class-specific quantile. Our results show that most normalization methods are biased when there is unbalanced data, a phenomenon where the percentages of up- and downregulated metabolites are unequal. Notably, unbalanced data can be sourced from the underlying biological differences, experimental perturbations, and metabolic interference. Beyond normalization algorithms and data structure, our study also emphasizes the importance of considering additional factors contributed by data quality, such as background noise, signal saturation, and missingness. Based on these findings, we propose an evidence-based normalization strategy to maximize sample normalization outcomes, providing a robust bioinformatic solution for advancing metabolomics research with a fair quantitative comparison.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"702–711 702–711"},"PeriodicalIF":4.6,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Insulin Measurement to Partial Exocytosis Model: Advances in Single Pancreatic Beta Cell Amperometry over Four Decades. 从胰岛素测量到部分胞吐模型：四十年来单个胰腺β细胞电流测量的进展。

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-10 eCollection Date: 2024-12-18 DOI: 10.1021/acsmeasuresciau.4c00058

Amir Hatamie, Xiulan He, Andrew Ewing, Patrik Rorsman

{"title":"From Insulin Measurement to Partial Exocytosis Model: Advances in Single Pancreatic Beta Cell Amperometry over Four Decades.","authors":"Amir Hatamie, Xiulan He, Andrew Ewing, Patrik Rorsman","doi":"10.1021/acsmeasuresciau.4c00058","DOIUrl":"10.1021/acsmeasuresciau.4c00058","url":null,"abstract":"Single cell Amperometry (SCA) is a powerful, sensitive, high temporal resolution electrochemical technique used to quantify secreted molecular messengers from individual cells and vesicles. This technique has been extensively applied to study the process of exocytosis, and it has also been applied, albeit less frequently, to investigate insulin exocytosis from single pancreatic beta cells. Insufficient insulin release can lead to diabetes, a chronic lifestyle disorder that affects millions of people worldwide. This review aims to summarize and highlight electrochemical measurements of insulin via monitoring its secretion from beta cells by SCA with micro- and nanoelectrodes since the 1990s and to explain how and why serotonin is used as a proxy for monitoring insulin during exocytosis from single beta cells. Finally, we describe how the combination of SCA measurements with the intracellular vesicle impact electrochemical cytometry (IVIEC) technique has led to important findings regarding fractional release types in beta cells. These findings, reported recently, have opened a new window in the study of pore formation, exocytosis from single vesicles, and the mechanisms of insulin secretion. This sensitive cellular electroanalysis approach should help in the development of novel therapeutic strategies targeting diabetes in the future.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"629-637"},"PeriodicalIF":4.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gas-Liquid-Solid Three-Phase Boundary in Scanning Electrochemical Cell Microscopy. 扫描电化学细胞显微镜中的气-液-固三相边界。

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-10 eCollection Date: 2024-12-18 DOI: 10.1021/acsmeasuresciau.4c00061

C Hyun Ryu, Debasree Mandal, Hang Ren

{"title":"Gas-Liquid-Solid Three-Phase Boundary in Scanning Electrochemical Cell Microscopy.","authors":"C Hyun Ryu, Debasree Mandal, Hang Ren","doi":"10.1021/acsmeasuresciau.4c00061","DOIUrl":"10.1021/acsmeasuresciau.4c00061","url":null,"abstract":"The gas-liquid-solid interface plays a crucial role in various electrochemical energy conversion devices, including fuel cells and electrolyzers. Understanding the effect of gas transfer on the electrochemistry at this three-phase interface is a grand challenge. Scanning electrochemical cell microscopy (SECCM) is an emerging technique for mapping the heterogeneity in electrochemical activity; it also inherently features a three-phase boundary at the nanodroplet cell. Herein, we quantitatively analyze the role of the three-phase boundary in SECCM involving gas via finite element simulation. Oxygen reduction reaction is used as an example for reaction with a gas reactant, which shows that interfacial gas transfer can enhance the overall mass transport of reactant, allowing measuring current density of several A/cm2. The hydrogen evolution reaction is used as an example for reaction with a gas product, and fast interfacial gas transfer kinetics can significantly reduce the concentration of dissolved gas near the electrode. This helps to measure electrode kinetics at a high current density without the complication of gas bubble formation. The contribution of interfacial gas transfer can be understood by directly comparing its kinetics to the mass transfer coefficient from the solution. Our findings aid the quantitative application of SECCM in studying electrochemical reactions involving gases, establishing a basis for investigating electrochemistry at the three-phase boundary.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"729-736"},"PeriodicalIF":4.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Insulin Measurement to Partial Exocytosis Model: Advances in Single Pancreatic Beta Cell Amperometry over Four Decades 从胰岛素测量到部分胞吐模型：四十年来单个胰腺β细胞电流测量的进展

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-10 DOI: 10.1021/acsmeasuresciau.4c0005810.1021/acsmeasuresciau.4c00058

Amir Hatamie*, Xiulan He, Andrew Ewing and Patrik Rorsman,

{"title":"From Insulin Measurement to Partial Exocytosis Model: Advances in Single Pancreatic Beta Cell Amperometry over Four Decades","authors":"Amir Hatamie*, Xiulan He, Andrew Ewing and Patrik Rorsman, ","doi":"10.1021/acsmeasuresciau.4c0005810.1021/acsmeasuresciau.4c00058","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00058https://doi.org/10.1021/acsmeasuresciau.4c00058","url":null,"abstract":"Single cell Amperometry (SCA) is a powerful, sensitive, high temporal resolution electrochemical technique used to quantify secreted molecular messengers from individual cells and vesicles. This technique has been extensively applied to study the process of exocytosis, and it has also been applied, albeit less frequently, to investigate insulin exocytosis from single pancreatic beta cells. Insufficient insulin release can lead to diabetes, a chronic lifestyle disorder that affects millions of people worldwide. This review aims to summarize and highlight electrochemical measurements of insulin via monitoring its secretion from beta cells by SCA with micro- and nanoelectrodes since the 1990s and to explain how and why serotonin is used as a proxy for monitoring insulin during exocytosis from single beta cells. Finally, we describe how the combination of SCA measurements with the intracellular vesicle impact electrochemical cytometry (IVIEC) technique has led to important findings regarding fractional release types in beta cells. These findings, reported recently, have opened a new window in the study of pore formation, exocytosis from single vesicles, and the mechanisms of insulin secretion. This sensitive cellular electroanalysis approach should help in the development of novel therapeutic strategies targeting diabetes in the future.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"629–637 629–637"},"PeriodicalIF":4.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gas–Liquid–Solid Three-Phase Boundary in Scanning Electrochemical Cell Microscopy 扫描电化学细胞显微镜中的气-液-固三相边界

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-10 DOI: 10.1021/acsmeasuresciau.4c0006110.1021/acsmeasuresciau.4c00061

C. Hyun Ryu, Debasree Mandal and Hang Ren*,

{"title":"Gas–Liquid–Solid Three-Phase Boundary in Scanning Electrochemical Cell Microscopy","authors":"C. Hyun Ryu, Debasree Mandal and Hang Ren*, ","doi":"10.1021/acsmeasuresciau.4c0006110.1021/acsmeasuresciau.4c00061","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00061https://doi.org/10.1021/acsmeasuresciau.4c00061","url":null,"abstract":"The gas–liquid–solid interface plays a crucial role in various electrochemical energy conversion devices, including fuel cells and electrolyzers. Understanding the effect of gas transfer on the electrochemistry at this three-phase interface is a grand challenge. Scanning electrochemical cell microscopy (SECCM) is an emerging technique for mapping the heterogeneity in electrochemical activity; it also inherently features a three-phase boundary at the nanodroplet cell. Herein, we quantitatively analyze the role of the three-phase boundary in SECCM involving gas via finite element simulation. Oxygen reduction reaction is used as an example for reaction with a gas reactant, which shows that interfacial gas transfer can enhance the overall mass transport of reactant, allowing measuring current density of several A/cm2. The hydrogen evolution reaction is used as an example for reaction with a gas product, and fast interfacial gas transfer kinetics can significantly reduce the concentration of dissolved gas near the electrode. This helps to measure electrode kinetics at a high current density without the complication of gas bubble formation. The contribution of interfacial gas transfer can be understood by directly comparing its kinetics to the mass transfer coefficient from the solution. Our findings aid the quantitative application of SECCM in studying electrochemical reactions involving gases, establishing a basis for investigating electrochemistry at the three-phase boundary.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"729–736 729–736"},"PeriodicalIF":4.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ready-To-Use Microwave Sensor Modified by Antibody-AuNPs Nanoconjugate for Highly Sensitive and Selective Detection of the SARS-CoV-2 Virus 抗体- aunps纳米偶联修饰的即用型微波传感器，用于高灵敏度和选择性检测SARS-CoV-2病毒

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-09 DOI: 10.1021/acsmeasuresciau.4c0003210.1021/acsmeasuresciau.4c00032

Jin Wang, Weijia Cui, Carolyn L. Ren and Emmanuel A. Ho*,

{"title":"Ready-To-Use Microwave Sensor Modified by Antibody-AuNPs Nanoconjugate for Highly Sensitive and Selective Detection of the SARS-CoV-2 Virus","authors":"Jin Wang, Weijia Cui, Carolyn L. Ren and Emmanuel A. Ho*, ","doi":"10.1021/acsmeasuresciau.4c0003210.1021/acsmeasuresciau.4c00032","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00032https://doi.org/10.1021/acsmeasuresciau.4c00032","url":null,"abstract":"The COVID-19 outbreak has led to notable developments in point-of-care (POC) diagnostic devices, as they can be valuable resources in identifying and managing the spread of the pandemic. Currently, the majority of techniques demand advanced laboratory equipment and professionals to execute precise, efficient, accurate, and sensitive testing. In this work, we report a new method to significantly enhance the sensitivity of microwave sensing of the SARS-CoV-2 virus by functionalizing the sensor surface using anti-SARS-CoV-2 spike antibody-gold nanoparticle (AuNPs) conjugates. AuNPs were surface-functionalized with the antispike antibody by EDC/NHS chemistry via PEG as a linker to form the conjugate (Ab-PEG-AuNPs). The Ab-PEG-AuNPs nanoconjugate was then coated onto the sensor through APTES and used for selectively capturing the spike protein on the SARS-CoV-2 virus. The sensing performance of the modified sensor was demonstrated via both experimental measurements and numerical simulations. Our sensor exhibited high sensitivity, achieving a limit of detection of 1,000 copies/mL of the SARS-CoV-2 virus within a 60 min time frame while requiring a minimal sample volume of 100 μL. The sensor exhibits outstanding specificity in distinguishing SARS-CoV-2 from other viruses, including influenza A and B, SARS-CoV-1, and MERS-CoV. Overall, this sensor provides a sensitive and label-free alternative for COVID-19 POC diagnosis.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"651–658 651–658"},"PeriodicalIF":4.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ready-To-Use Microwave Sensor Modified by Antibody-AuNPs Nanoconjugate for Highly Sensitive and Selective Detection of the SARS-CoV-2 Virus. 抗体- aunps纳米偶联修饰的即用型微波传感器，用于高灵敏度和选择性检测SARS-CoV-2病毒。

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-09 eCollection Date: 2024-12-18 DOI: 10.1021/acsmeasuresciau.4c00032

Jin Wang, Weijia Cui, Carolyn L Ren, Emmanuel A Ho

{"title":"Ready-To-Use Microwave Sensor Modified by Antibody-AuNPs Nanoconjugate for Highly Sensitive and Selective Detection of the SARS-CoV-2 Virus.","authors":"Jin Wang, Weijia Cui, Carolyn L Ren, Emmanuel A Ho","doi":"10.1021/acsmeasuresciau.4c00032","DOIUrl":"10.1021/acsmeasuresciau.4c00032","url":null,"abstract":"The COVID-19 outbreak has led to notable developments in point-of-care (POC) diagnostic devices, as they can be valuable resources in identifying and managing the spread of the pandemic. Currently, the majority of techniques demand advanced laboratory equipment and professionals to execute precise, efficient, accurate, and sensitive testing. In this work, we report a new method to significantly enhance the sensitivity of microwave sensing of the SARS-CoV-2 virus by functionalizing the sensor surface using anti-SARS-CoV-2 spike antibody-gold nanoparticle (AuNPs) conjugates. AuNPs were surface-functionalized with the antispike antibody by EDC/NHS chemistry via PEG as a linker to form the conjugate (Ab-PEG-AuNPs). The Ab-PEG-AuNPs nanoconjugate was then coated onto the sensor through APTES and used for selectively capturing the spike protein on the SARS-CoV-2 virus. The sensing performance of the modified sensor was demonstrated via both experimental measurements and numerical simulations. Our sensor exhibited high sensitivity, achieving a limit of detection of 1,000 copies/mL of the SARS-CoV-2 virus within a 60 min time frame while requiring a minimal sample volume of 100 μL. The sensor exhibits outstanding specificity in distinguishing SARS-CoV-2 from other viruses, including influenza A and B, SARS-CoV-1, and MERS-CoV. Overall, this sensor provides a sensitive and label-free alternative for COVID-19 POC diagnosis.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"651-658"},"PeriodicalIF":4.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Electrochemiluminescence Detection of Dopamine Using Antifouling PEDOT-Modified SPEs for Complex Biological Samples. 利用防污pedot修饰spe增强电化学发光检测复杂生物样品中的多巴胺。

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-04 eCollection Date: 2024-12-18 DOI: 10.1021/acsmeasuresciau.4c00053

Tzu-Yu Kao, Chia-Hung Kuo, Yu-Wei Wu, Shyh-Chyang Luo

{"title":"Enhanced Electrochemiluminescence Detection of Dopamine Using Antifouling PEDOT-Modified SPEs for Complex Biological Samples.","authors":"Tzu-Yu Kao, Chia-Hung Kuo, Yu-Wei Wu, Shyh-Chyang Luo","doi":"10.1021/acsmeasuresciau.4c00053","DOIUrl":"10.1021/acsmeasuresciau.4c00053","url":null,"abstract":"Detecting medically important biomarkers in complex biological samples without prior treatment or extraction poses a major challenge in biomedical analysis. Electrochemical methods, specifically electrochemiluminescence (ECL), show potential due to their high sensitivity, minimal background noise, and straightforward operation. This study investigates the ECL performance of screen-printed electrodes (SPEs) modified with the conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and its derivatives for dopamine (DA) detection. PEDOT modification significantly enhances ECL intensity, improves sensitivity, and expands the linear range for DA detection. Functionalizing PEDOT with ethylene glycol (EG) further enhances stability, specificity, and resistance to interferences for DA detection. These modified SPEs demonstrate the linear range of 1-200 μM and a detection limit as low as 0.887 nM (S/N = 3), surpassing many previous studies using SPEs. Moreover, the PEDOT-EG4-OMe-modified SPEs can reliably detect DA in solutions with high protein concentrations or artificial cerebrospinal fluid. These results suggest that the PEDOT derivative-modified SPE can serve as reusable and sensitive DA sensors in complex biological environments, highlighting the potential of the ECL system for a range of challenging applications.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"712-720"},"PeriodicalIF":4.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simplifying Wheat Quality Assessment: Using Near-Infrared Spectroscopy and Analysis of Variance Simultaneous Component Analysis to Study Regional and Annual Effects 简化小麦品质评价：利用近红外光谱和方差分析同时成分分析研究区域和年度效应

IF 4.6

ACS Measurement Science Au Pub Date : 2024-10-04 DOI: 10.1021/acsmeasuresciau.4c0004410.1021/acsmeasuresciau.4c00044

Stephan Freitag*, Maximilian Anlanger, Maximilian Lippl, Klemens Mechtler, Elisabeth Reiter, Heinrich Grausgruber and Rudolf Krska,

{"title":"Simplifying Wheat Quality Assessment: Using Near-Infrared Spectroscopy and Analysis of Variance Simultaneous Component Analysis to Study Regional and Annual Effects","authors":"Stephan Freitag*, Maximilian Anlanger, Maximilian Lippl, Klemens Mechtler, Elisabeth Reiter, Heinrich Grausgruber and Rudolf Krska, ","doi":"10.1021/acsmeasuresciau.4c0004410.1021/acsmeasuresciau.4c00044","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00044https://doi.org/10.1021/acsmeasuresciau.4c00044","url":null,"abstract":"Assessing the quality of wheat, one of humanity’s most important crops, in a straightforward manner, is essential. In this study, analysis of variance (ANOVA) simultaneous component analysis (ASCA) paired with near-infrared spectroscopy (NIRS) was used as an easy-to-implement and environmentally friendly tool for this purpose. The capabilities of combining NIRS with ASCA were demonstrated by studying the effects of sampling site and year on the quality of 180 Austrian wheat samples across four sites over 3 years. It was found that the year, sample site, and their combination significantly (p < 0.001) affect the NIR spectra of wheat. NIR spectral preprocessing tools, usually employed in chemometric workflows, notably influence the results obtained by ASCA, particularly in terms of the variance attributed to annual and regional effects. The influence of the year was identified as the dominant factor, followed by region and the combined effect of year and sampling site. Interpretation of the loading plots obtained by ASCA demonstrates that wheat components such as proteins, carbohydrates, moisture, or fat contribute to annual and regional differences. Additionally, the protein, starch, moisture, fat, fiber, and ash contents of wheat samples obtained using a NIR-based calibration were found to be significantly influenced by year, sampling site, or their combination using ANOVA. This study shows that the combination of ASCA with NIRS simplifies NIR-based quality assessment of wheat without the need for time- and chemical-consuming calibration development.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"695–701 695–701"},"PeriodicalIF":4.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142850705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0