Fhysmélia Firmino de Albuquerque, Rodrigo Michelin Iost and Frank Nelson Crespilho*,
{"title":"Comparative Roles of Hydrogels, Deep Eutectic Solvents, and Ionic Liquids in Enzyme-Based Biosensors, Bioelectronics and Biomimetics Devices","authors":"Fhysmélia Firmino de Albuquerque, Rodrigo Michelin Iost and Frank Nelson Crespilho*, ","doi":"10.1021/acsmeasuresciau.5c00036","DOIUrl":null,"url":null,"abstract":"<p >The development of enzyme-based bioelectronic devices, including biosensors and biomimetic systems, has significantly advanced with the introduction of innovative materials such as hydrogels, deep eutectic solvents (DES), and ionic liquids (ILs). These materials offer unique advantages in enhancing biodevice performance, particularly in enzyme stabilization, biocompatibility, and electrochemical sensitivity. Hydrogels, known for their high water content and flexibility, provide an ideal matrix for enzyme immobilization in biological applications but are limited by low ionic conductivity. DES, with their green chemistry credentials and ability to stabilize enzymes under harsh conditions, show great promise, although scalability and performance in complex biological systems remain challenges. ILs, with their superior electron transfer capabilities, enable high sensitivity in electrochemical biosensors, though issues of viscosity and potential toxicity need to be addressed for broader biomedical use. This review provides a comparative analysis of the roles of these materials in enzyme-based biosensors and bioelectronics, including microbatteries and bioelectrosynthesis, highlighting their respective strengths, limitations, and future opportunities. The integration of these materials holds great potential for advancing bioelectronics technologies, with applications spanning medical diagnostics, environmental monitoring, and industrial processes. By addressing current challenges and optimizing these materials for large-scale use, the future of enzyme-based devices could see significant improvements in efficiency, sensitivity, and sustainability.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 4","pages":"424–442"},"PeriodicalIF":4.6000,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsmeasuresciau.5c00036","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Measurement Science Au","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsmeasuresciau.5c00036","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The development of enzyme-based bioelectronic devices, including biosensors and biomimetic systems, has significantly advanced with the introduction of innovative materials such as hydrogels, deep eutectic solvents (DES), and ionic liquids (ILs). These materials offer unique advantages in enhancing biodevice performance, particularly in enzyme stabilization, biocompatibility, and electrochemical sensitivity. Hydrogels, known for their high water content and flexibility, provide an ideal matrix for enzyme immobilization in biological applications but are limited by low ionic conductivity. DES, with their green chemistry credentials and ability to stabilize enzymes under harsh conditions, show great promise, although scalability and performance in complex biological systems remain challenges. ILs, with their superior electron transfer capabilities, enable high sensitivity in electrochemical biosensors, though issues of viscosity and potential toxicity need to be addressed for broader biomedical use. This review provides a comparative analysis of the roles of these materials in enzyme-based biosensors and bioelectronics, including microbatteries and bioelectrosynthesis, highlighting their respective strengths, limitations, and future opportunities. The integration of these materials holds great potential for advancing bioelectronics technologies, with applications spanning medical diagnostics, environmental monitoring, and industrial processes. By addressing current challenges and optimizing these materials for large-scale use, the future of enzyme-based devices could see significant improvements in efficiency, sensitivity, and sustainability.
期刊介绍:
ACS Measurement Science Au is an open access journal that publishes experimental computational or theoretical research in all areas of chemical measurement science. Short letters comprehensive articles reviews and perspectives are welcome on topics that report on any phase of analytical operations including sampling measurement and data analysis. This includes:Chemical Reactions and SelectivityChemometrics and Data ProcessingElectrochemistryElemental and Molecular CharacterizationImagingInstrumentationMass SpectrometryMicroscale and Nanoscale systemsOmics (Genomics Proteomics Metabonomics Metabolomics and Bioinformatics)Sensors and Sensing (Biosensors Chemical Sensors Gas Sensors Intracellular Sensors Single-Molecule Sensors Cell Chips Arrays Microfluidic Devices)SeparationsSpectroscopySurface analysisPapers dealing with established methods need to offer a significantly improved original application of the method.