ACS Measurement Science Au最新文献

{"title":"Chiral Twin-Electrodes for Amplifying the Faradaic Current of Enantiomers via Redox Cycling","authors":"Sorasak Klinyod,&nbsp;Zikkawas Pasom,&nbsp;Gerardo Salinas,&nbsp;Patrick Garrigue,&nbsp;Véronique Lapeyre,&nbsp;Stéphane Reculusa,&nbsp;Chularat Wattanakit,&nbsp;Alexander Kuhn","doi":"10.1021/acsmeasuresciau.6c00107","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00107","url":null,"abstract":"<p>Redox cycling (RC)is often used to amplify the faradaic currentassociated with the electrochemical transformation of redox-activespecies. For a given concentration, this allows a considerable increasein sensitivity and an improvement in the detection limit of analyticaldevices. However, all previous reports have focused only on the detectionof achiral molecules, e.g., ferrocene derivatives. In this contribution,we report an original setup composed of two metal electrodes encodedwith chiral features, facing each other to form a microchannel. Thepresence of the same chiral feature on the two parallel-oriented electrodesenables the preferential cyclic transformation of a specific enantiomer.It will be more easily oxidized at the anode with the right chiralityand can subsequently diffuse across the microchannel to be reducedback into the starting enantiomer at a cathode with an identical chiralfeature. As a function of the width of the microchannel, this cycleis repeated multiple times, significantly enhancing the overall currentin terms of chiral recognition. Herein, we illustrate this fundamentalconcept with mesoporous Pt–Ir alloy electrodes, imprinted witheither L-DOPA or D-DOPA as transducers constituting the microchannel.The latter allows an amplification of the analytical signal of DOPAas a model compound by more than one order of magnitude and with anenantiodiscrimination efficiency reaching 90%. Thus, the proposedstrategy opens up very interesting perspectives for chiral-sensingapplications.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1263–1272"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-Free SERS Analysis of Glioma Cells to Detect Drug Resistance Using Metabolic Phenylalanine Level","authors":"Guohui Yang,&nbsp;Xin Wang,&nbsp;Jingbin Jin,&nbsp;Xiaozhang Qu,&nbsp;Kaizhi Zhang,&nbsp;Shuping Xu","doi":"10.1021/acsmeasuresciau.6c00014","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00014","url":null,"abstract":"<p>Gliomais one of the most common malignant brain tumors, and itsmainstream clinical treatment regimens mainly include postoperativecombined with Temozolomide chemotherapy. Unfortunately, glioma cellstend to mutate when subjected to prolonged Temozolomide treatment,leading to drug resistance and significantly weakening the therapeuticeffect. Delaying the resistance time of Temozolomide has become apressing issue for clinicians to address, and the primary regimenfor delaying drug resistance is the combined application of Temozolomidewith other therapies. To explore new molecular diagnostic markersof drug resistance in glioma and to assess treatment stage, surface-enhancedRaman spectroscopy (SERS) was used to investigate changes in gliomacells under combined therapies including physical (electrical stimulation,ES) and chemical (cancer drug: Temozolomide) treatments. By analyzingintensity changes in the SERS band at 997 cm^–1,we observed that glioma cells showed a higher phenylalanine (Phe)expression. Interestingly, dynamic variations in Phe content secretedfrom glioma cells were drug resistance-dependent. ES, a novel therapeutictechnique that can inhibit cell proliferation by promoting gliomacell apoptosis, was combined with Temozolomide. In both simple ESand ES plus Temozolomide conditions, metabolic Phe levels in gliomacells are significantly elevated, suggesting that Phe overexpressionin glioma cells can serve as a potential indicator for acceleratedcancer cell apoptosis. Our study proves that ES can effectively reduceTemozolomide doses, offering an easy-to-implement approach to delayingthe onset of drug resistance. This work not only reveals a possibleantidrug-resistance treatment strategy for glioma but also providesimportant guidance on a potential spectral indicator for early diagnosisof drug resistance, which is of significance for clinical applications.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"916–922"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amplification-Free Detection of MiRNA Signatures in Milk: A Noninvasive Sensing Approach for Early Diagnosis of Bovine Mastitis","authors":"Kandeel Shafique,&nbsp;Hedieh Haji-Hashemi,&nbsp;Łukasz Gontar,&nbsp;Ma̅ra Pilmane,&nbsp;Gergely Maróti,&nbsp;Beatriz Prieto-Simón","doi":"10.1021/acsmeasuresciau.6c00060","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00060","url":null,"abstract":"<p>Bovine mastitis is an infectious disease with a significantimpacton the dairy industry. It causes reduced milk yield and quality, andoccasional mortality. Early and accurate diagnosis is challenging,being especially critical for subclinical mastitis lacking visiblesymptoms. MicroRNAs (miRNAs) are emerging as promising biomarkersfor mastitis. Herein, using next-generation sequencing, a distinctmiRNA signature (miRNA-146b, miRNA-221, miRNA-223) is identified,which is differentially expressed in healthy and naturally infectedsubclinical cows. Next, electrochemical DNA sensors are developedby self-assembling methylene blue-tagged oligonucleotides, complementaryto each miRNA, on gold electrodes. MiRNA detection is achieved bymonitoring hybridization-induced conformational changes using square-wavevoltammetry. The sensors exhibit a sigmoidal response (dynamic range0.1–100 nM), with a limit of detection of 0.1 nM and excellentselectivity. Importantly, they demonstrate strong diagnostic performancein milk-derived RNA extracts, with receiver operating characteristic–areaunder the curve values of 0.88, 0.80, and 0.70 for miRNA-221, miRNA-146b,and miRNA-223, respectively. Moreover, the responses are biologicallymeaningful, as correlation analysis highlights distinct associationsof miRNA-146b and miRNA-221 with inflammation, and of miRNA-221 andmiRNA-223 with microbial burden. The amplification-free detectionof clinically relevant miRNAs in milk extracts represents a significantadvancement toward noninvasive testing for the early diagnosis ofmastitis. The development of simple, sensitive, and specific sensorstargeting unique signatures of host immune-derived biomarkers promisesa new framework to improve disease management and antimicrobial stewardship.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1091–1104"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measuring Electrode Kinetics Under Spatial Confinement. Application of Pulse Voltammetry to Different Mass Transport Modes Using Butler–Volmer and Marcus–Hush–Chidsey Frameworks","authors":"José V. Hernández-Tovar,&nbsp;Antonio J. Martínez-García,&nbsp;Francisco Martínez-Ortiz,&nbsp;Eduardo Laborda,&nbsp;Manuela López-Tenés,&nbsp;Joaquín González","doi":"10.1021/acsmeasuresciau.6c00096","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00096","url":null,"abstract":"<p>Spatial confinement arises in many electrochemical phenomena,suchas the electrochemical characterization of ultrathin membranes; insertionof ionic species into porous matrices; charge transfer processes inporous electrodes or through porous matrices; or redox cycling deviceswith bipotentiostatic control. In all these situations the mass transportoccurs in solution layers whose thickness is comparable to or smallerthan that of the diffusion layer. Two mass transport modalities canbe considered in this context of finite diffusion: one leading tothe partial or complete depletion of the electroactive species andthe other related to the faradaic regeneration of the redox species.Of special interest is the relationship between spatial confinementand kinetic influences on overall electrochemical responses. In thespecific case of redox kinetics, there is a lack of theoretical frameworksdeveloped specifically for these new experimental scenarios, in orderto provide simple and accurate tools that enable us to characterizethe kinetics at play. In fact, methodologies developed for semi-infinitediffusion (as, for example, the Nicholson method) are still beingapplied, even though they are clearly unsuitable in this case. NormalPulse Voltammetry (NPV) is proposed as a simple and accurate techniqueto characterize electrochemical processes with slow kinetics. Unusualfeatures arise in the current–potential responses due to theoverall kinetic dependence, which combines the effects of mass transportand redox kinetics. This allows all the key parameters of the responsesto be determined with great accuracy. The well-known Butler–Volmer(BV) and Marcus–Hush–Chidsey (MHC) formalisms have beenconsidered. This technique was applied to analyze the quasi-reversibleoxidation of 4-carboxy-2,2,6,6-tetramethylpiperidine-1-oxyl (4-carboxy-TEMPO)radicals in aqueous solutions using a boron-doped diamond electrode.MHC formalism was found to be more coherent when describing the electrochemicalresponses for both mass transport modes.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1239–1255"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 3D Printed Mini-Gel Electrophoresis System for Rapid and Inexpensive DNA Nanoswitch Biosensing","authors":"Vinod Morya,&nbsp;Andrew Hayden,&nbsp;Lifeng Zhou,&nbsp;Dadrian Cole,&nbsp;Ken Halvorsen","doi":"10.1021/acsmeasuresciau.6c00041","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00041","url":null,"abstract":"<p>Gel electrophoresishas been a cornerstone laboratorytechniquefor decades, yet it is often viewed as cumbersome and costly and confinedto laboratory settings. Recent advances in DNA nanotechnology haveused electrophoresis as a primary readout for some biosensing applicationssuch as DNA nanoswitches, where a conformational change in a DNA structureindicates the presence of a target molecule. Conventional gel electrophoresissetups are not ideal for such targeted applications, with moderateequipment cost, excessive reagent use, and time-consuming processes.Here, we adopt a reductionist, application-driven approach to redesigngel electrophoresis specifically for DNA-nanoswitch-based detection.We present a fully 3D printable mini-gel electrophoresis system thatincorporates conductive plastic electrodes, demonstrating performancecomparable to conventional systems using platinum electrodes. By optimizingthe interelectrode distance and running parameters, our system resolvesthe on/off states of DNA nanoswitches in as little as 1 min. We furthershow that the device operates reliably at low voltages, includingwhen powered by a USB power bank, and even enables instrument-freenanoswitch readout using an LED with a cell phone camera. Our designsubstantially reduces the cost, voltage requirements, material usage,operational complexity, and experimental time. These improvementsmake gel-based biosensing more practical outside traditional laboratoryenvironments, paving the way for the broader adoption of gel electrophoresisin point-of-care and resource-limited settings.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1049–1055"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Sensitivity Magnetic Particle Spectroscopy Platform for Precise, Specific, and Rapid Detection of Infectious Disease and Cancer-Related Biomarkers","authors":"Hafiz Ashfaq Ahmad,&nbsp;Ali Dinari,&nbsp;Minh Phu Bui,&nbsp;Seungjun Oh,&nbsp;Masoud Rezaie,&nbsp;Shahla Kianamiri,&nbsp;Jungwon Yoon","doi":"10.1021/acsmeasuresciau.6c00032","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00032","url":null,"abstract":"<p>Accuratebiosensing using magnetic particle spectroscopy (MPS)depends on the interplay between biomolecular binding and the fidelityof the measurement system. MPS enables wash-free, label-free biosensingby monitoring Brownian relaxation-induced changes in magnetic nanoparticle(MNP) dynamics. However, its practical performance is often limitedby a weak signal-to-noise ratio, excitation feedthrough, environmentalnoise, slow acquisition, and insufficient mass sensitivity. Here,a gradiometer-based MPS system incorporating noise suppression, signalintegration, and harmonic detection was developed to monitor relaxationchanges upon biomolecular binding. Single-core SHP-25 MNPs were conjugatedwith recombinant human angiotensin-converting enzyme 2 (ACE2) protein,antinucleoprotein (anti_NP) antibodies, and AS1411 aptamer to targetthe SARS-CoV-2 receptor-binding domain (RBD), influenza A hemagglutinintype-1, neuraminidase type-1 (H1N1) nucleoprotein, and breast cancerbiomarker nucleolin, respectively. Conjugation was validated by FT-IRspectroscopy and DLS to confirm chemical attachment and hydrodynamicsize modulation, while ICP-MS was used for iron-mass normalization.Relaxation changes from conjugation and target binding were recordedby MPS. The third-harmonic showed the highest sensitivity to binding-inducedrelaxation delays, with detection limits of 1 nM (100 fmol) for ACE2and anti_NP and 12 nM for AS1411. Target-binding yielded statisticallysignificant responses at concentrations as low as 5 nM for RBD, H1N1,and nucleolin. The harmonic ratio R₅₃ (fifth/third) increasedmonotonically with binding, providing a mass-normalized recognitionmetric with reduced dependence on nanoparticle concentration. Thesystem achieved an iron-mass sensitivity of 1 ng Fe. Comparative analysisshows that monoclonal antibody-conjugated MNPs provide greater specificityand quantitative robustness in MPS measurements than polyclonal antibodies.The high sensitivity of the developed MPS, combined with the superiorspecificity of monoclonal antibodies, enables precise biosensing withpotential clinical relevance.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1023–1037"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MetabolEx: Platform for Small-Molecule Metabolites Generation and Metabolic Pathway Prediction","authors":"Filippo Lunghini,&nbsp;Giada Agazzi,&nbsp;Valerio Tazzari,&nbsp;Anna Fava,&nbsp;Serena Vittorio,&nbsp;Alessio Macorano,&nbsp;Giulio Vistoli,&nbsp;Andrea Rosario Beccari","doi":"10.1021/acsmeasuresciau.6c00019","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00019","url":null,"abstract":"<p>Accurate metabolite prediction is essential for identifyingsafetyliabilities and optimizing pharmacokinetic properties during earlydrug discovery. However, predicting metabolic fate remains a majorbottleneck due to the risk of unexpected toxicity and drug–druginteractions. We present MetabolEx, a platform that integrates rule-basedgeneration with machine learning prioritization to predict Phase Iand II metabolism. MetabolEx utilizes 194 manually curated reactionsin SMIRKS notation and machine learning classifiers trained on 41,041substrate-metabolite pairs from multiple sources. This hybrid approachenumerates chemically plausible metabolites, while applying probabilisticfiltering to manage the combinatorial complexity of recursive prediction.External validation on 219 substrates demonstrated the platform’sperformance across three recursive prediction levels. At L1, MetabolExachieved 0.84 recall for the primary metabolites. At L2, the toolreached double the precision of state-of-the-art methods with a significantreduction in false positives (FP) while maintaining a substantialrecall of 0.57. At L3, MetabolEx achieved 5-fold higher precisionand a 25-fold reduction in FP compared to exhaustive enumeration,while maintaining a recall of 0.35. These results demonstrate thatprobabilistic filtering converts high-volume metabolic output intoa focused and manageable set of predictions. MetabolEx captures complextransformations across eight reaction classes, including heterocyclemetabolism and glutathione conjugation, and serves as a comprehensiveresource for metabolic and toxicological profiling. The platform featuresinteractive network visualization and automated toxicity annotationand is freely available at https://metabolex.exscalate.eu.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"998–1010"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Anomeric Mannopentaose-Based Nonenzymatic NIR Glucose Sensor Enabling Cost-Effective Continuous Monitoring","authors":"Sayantan Tripathy,&nbsp;Diana Al Husseini,&nbsp;Carolina I. Martinez,&nbsp;Anthony Dongchau,&nbsp;Samyucktha Ganesapandian,&nbsp;Melissa A Grunlan,&nbsp;Gerard L Cote","doi":"10.1021/acsmeasuresciau.6c00076","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00076","url":null,"abstract":"<p>In this study, we present a novel, fully injectable,nonenzymaticglucose biosensing platform that uses NIR fluorescence from a PEGylated-ConAand Cy5.5-β-mannopentaose complex to achieve highly sensitiveand stable detection. The proof-of-concept assay is designed for highsensitivity, long-term stability, a small size to permit injectabilityor minimal invasiveness, suitability across all skin tones, and cost-effectiveness.As a control, an analogous assay was prepared with the α-anomericform (i.e., Cy5.5-α-mannopentaose), which is 10X costlier. Usingfluorescence anisotropy, a computational model was developed to optimizethe concentration ratios of assay components for sensitive glucosedetection within the physiological range. Both conjugates demonstratedcomparable responsiveness across physiological glucose concentrations,with Cy5.5-β-mannopentaose exhibiting a broader dynamic range(25–400 mg/dL) in buffer and comparable binding affinity. Inaddition, performance was validated in artificial interstitial fluidwith Cy5.5-β-mannopentaose showing reproducible solvatochromismacross the full blood glucose range (25–400 mg/dL), with strongresponsiveness maintained within the physiologically relevant subcutaneouswindow. To further validate its ability to be housed in a membranecarrier, the Cy5.5-β-mannopentaose solution was placed in thecavity of a hollow hydrogel rod with known antifouling propertiesand mesh size, and &gt;94% was retained over a 7-day period. Theseresultsestablish β-mannopentaose as a promising low-cost ligand forConA-based glucose sensing systems and support its potential use inthe future development of minimally invasive and scalable glucosesensing approaches.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1179–1193"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atomically Dispersed Nickel in Cerium Oxide Aerogel Catalysts Characterized Using Scanning Transmission Electron Microscopy and Spectroscopy","authors":"Kyle Sendgikoski,&nbsp;Austin E. Herzog,&nbsp;James L. Hart,&nbsp;Andrew C. Lang,&nbsp;Travis G. Novak,&nbsp;Michelle D. Johannes,&nbsp;Debra R. Rolison,&nbsp;Todd H. Brintlinger","doi":"10.1021/acsmeasuresciau.5c00189","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00189","url":null,"abstract":"<p>Catalytically active nickel-substituted cerium oxide(CeO₂) aerogels, engineered to maximize yield in the water–gasshift reaction (WGS: H₂O + CO ↔ H₂ +CO₂), are characterized using scanning transmission electronmicroscopy (STEM), electron energy-loss spectroscopy (EELS), and energy-dispersivespectroscopy (EDS). STEM shows that the architected CeO₂ catalysts with Ni substituted at 2.5, 5, and 10 atomic percentage(at.%) contain atomically distributed Ni within the ∼7 nm CeO₂ nanocrystalline domains that comprise the covalently bondedoxide network. The aerogels prepared at the two higher Ni-to-Ce compositionsalso contain &gt;100 nm nickel-oxide particles, which correlates withour prior report that these catalysts generate undesired CH₄ as a byproduct during WGS reactions run at mild temperatures. Themicrographic absence of large nickel-oxide particles in 2.5 at. %Ni-substituted CeO₂ aerogel substantiates why this WGScatalyst is methane-free to the limit of detection. We find that highelectron fluence modifies the cerium oxidation state and nickel distribution,resulting in a mix of Ce³⁺ and Ce⁴⁺ and ∼1nm nickel aggregates. Density functional theory indicates that Nications are mobile and tend to aggregate in a CeO₂ fluoritelattice, even in the absence of electron flux, particularly when locatednear oxygen vacancies. The nature of Ni within the CeO₂ aerogel, as observed by STEM/EELS/EDS, validates the need to preventNi aggregation in WGS catalysts.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"929–936"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Changes Underpinning Permethrin Resistance in the Anopheles gambiae Vector from Cameroon","authors":"Steve V. Djova,&nbsp;Michael W. Christopher,&nbsp;Sulaiman S. Ibrahim,&nbsp;Mersimine Kouamo,&nbsp;Rhoel R. Dinglasan,&nbsp;Timothy J. Garrett,&nbsp;Charles S. Wondji","doi":"10.1021/acsmeasuresciau.6c00054","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00054","url":null,"abstract":"<p>Insecticide resistance reduces the effectiveness of currentmalaria-vector-controlinterventions. To understand the mechanisms underlying permethrinresistance in field-derived <em>Anopheles gambiae</em> vectors collected from Cameroon, we used ultra-high-performanceliquid chromatography coupled with high-resolution tandem mass spectrometry(UHPLC-HRMS/MS) to comprehensively analyze metabolic changes in resistantand susceptible samples to gain insights into mechanisms driving permethrinresistance. Resistant mosquitoes exhibited upregulated levels of inosine,nicotinic acid, dipeptides, amino acids, fumarate, uracil, and aldopentoses.These data suggest that altered metabolic-based detoxification, aswell as target site and metabolic shifts, and enhanced energy productioncontribute to permethrin resistance. Conversely, susceptible mosquitoesshowed increased levels of <em>N</em>-acetyl-aspartic acid,xanthurenic acid, 2-hydroxyglutarate, 3-hydroxykynurenine, propanoylcarnitine,and <span>l</span>-pipecolic acid. These metabolites are associated withneurotoxicity, energy disruption, as well as tryptophan and lysinecatabolism, leading to susceptible phenotypes. These findings providedeeper metabolic insight into <em>An. gambiae</em> permethrin resistance and compel further investigations into howthese metabolic pathways contribute to the emergence of pyrethroidcross-resistance.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1081–1090"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}