ACS Measurement Science Au最新文献

{"title":"Transient Single Cell Hypoxia Induced by Localized Galvanostatic Oxygen Challenge","authors":"Marlene H. Hill,&nbsp;Gabriel N. Meloni,&nbsp;Bruno G. Frenguelli and Patrick R. Unwin*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0010010.1021/acsmeasuresciau.4c00100","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00100https://doi.org/10.1021/acsmeasuresciau.4c00100","url":null,"abstract":"<p >Studying cells exposed to low and controllable oxygen levels is key to investigating various fundamental aspects of pathological states, such as stroke and cancer. At present, available methodologies applied in vitro focus on large groups of cells exposed to low oxygen conditions through slow-time approaches, such as environmental incubators or microfluidic devices. Here, we demonstrate a novel approach for titrating the local oxygen concentration around individual adhered PC12 cells, enabling single cells within a population to be exposed to hypoxic-like conditions. A 25 μm diameter platinum disk microelectrode performing the oxygen reduction reaction (ORR) at constant current (galvanostatic control) is used as a microscale oxygen scavenger that can be positioned precisely over individual cells. By coupling the galvanostatic oxygen challenge with confocal laser scanning microscopy (CLSM) and a commercially available hypoxia dye (Image-iT Green hypoxia reagent), we monitor the response of single cells when exposed to depleted oxygen concentrations over time. Numerical simulations are used to characterize the oxygen and pH gradient imposed by the microelectrode at different cathodic currents, revealing that within seconds, the oxygen depletion zone reaches a steady-state condition, extending a few microelectrode radii into solution, while the corresponding pH gradient is strongly compressed by the buffer solution. Cells under the microelectrode show a marked increase in average fluorescence rate relative to control, reporting their hypoxic conditions and demonstrating the effectiveness of the proposed method. Heterogenous cell response in a challenged group is also observed, highlighting the ability of this approach to investigate the natural heterogeneity in cell populations. This work provides a platform and roadmap for future studies of cellular systems where the ability to control and vary oxygen concentration on a rapid time scale would be beneficial.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"234–241 234–241"},"PeriodicalIF":4.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transient Single Cell Hypoxia Induced by Localized Galvanostatic Oxygen Challenge.","authors":"Marlene H Hill, Gabriel N Meloni, Bruno G Frenguelli, Patrick R Unwin","doi":"10.1021/acsmeasuresciau.4c00100","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00100","url":null,"abstract":"<p><p>Studying cells exposed to low and controllable oxygen levels is key to investigating various fundamental aspects of pathological states, such as stroke and cancer. At present, available methodologies applied in vitro focus on large groups of cells exposed to low oxygen conditions through slow-time approaches, such as environmental incubators or microfluidic devices. Here, we demonstrate a novel approach for titrating the local oxygen concentration around individual adhered PC12 cells, enabling single cells within a population to be exposed to hypoxic-like conditions. A 25 μm diameter platinum disk microelectrode performing the oxygen reduction reaction (ORR) at constant current (galvanostatic control) is used as a microscale oxygen scavenger that can be positioned precisely over individual cells. By coupling the galvanostatic oxygen challenge with confocal laser scanning microscopy (CLSM) and a commercially available hypoxia dye (Image-iT Green hypoxia reagent), we monitor the response of single cells when exposed to depleted oxygen concentrations over time. Numerical simulations are used to characterize the oxygen and pH gradient imposed by the microelectrode at different cathodic currents, revealing that within seconds, the oxygen depletion zone reaches a steady-state condition, extending a few microelectrode radii into solution, while the corresponding pH gradient is strongly compressed by the buffer solution. Cells under the microelectrode show a marked increase in average fluorescence rate relative to control, reporting their hypoxic conditions and demonstrating the effectiveness of the proposed method. Heterogenous cell response in a challenged group is also observed, highlighting the ability of this approach to investigate the natural heterogeneity in cell populations. This work provides a platform and roadmap for future studies of cellular systems where the ability to control and vary oxygen concentration on a rapid time scale would be beneficial.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"234-241"},"PeriodicalIF":4.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Tutorial for Scanning Electrochemical Cell Microscopy (SECCM) Measurements: Step-by-Step Instructions, Visual Resources, and Guidance for First Experiments","authors":"Kamsy Lerae Anderson,&nbsp; and ,&nbsp;Martin Andrew Edwards*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0009110.1021/acsmeasuresciau.4c00091","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00091https://doi.org/10.1021/acsmeasuresciau.4c00091","url":null,"abstract":"<p >Scanning electrochemical cell microscopy (SECCM) produces nanoscale-resolution electrochemical maps of electrode surfaces using the meniscus at the tip of an electrolyte-filled nanopipette as a mobile electrochemical cell. While the use and range of applications of SECCM have grown rapidly since its introduction, the pathway to performing SECCM measurements can be daunting to those without direct access to expert users. This work fills this expertise gap by providing a step-by-step guide to performing one’s first SECCM experiments, including troubleshooting strategies, videos/images, suggested parameters and experimental systems, and representative data (of both successful experiments and common problems). No background in SECCM is assumed and fundamentals are clearly explained at each stage with a rationale for the experimental steps provided. This work provides an entry point for the uninitiated to understand and use this powerful nanoscale electrochemical characterization technique.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"160–177 160–177"},"PeriodicalIF":4.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Tutorial for Scanning Electrochemical Cell Microscopy (SECCM) Measurements: Step-by-Step Instructions, Visual Resources, and Guidance for First Experiments.","authors":"Kamsy Lerae Anderson, Martin Andrew Edwards","doi":"10.1021/acsmeasuresciau.4c00091","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00091","url":null,"abstract":"<p><p>Scanning electrochemical cell microscopy (SECCM) produces nanoscale-resolution electrochemical maps of electrode surfaces using the meniscus at the tip of an electrolyte-filled nanopipette as a mobile electrochemical cell. While the use and range of applications of SECCM have grown rapidly since its introduction, the pathway to performing SECCM measurements can be daunting to those without direct access to expert users. This work fills this expertise gap by providing a step-by-step guide to performing one's first SECCM experiments, including troubleshooting strategies, videos/images, suggested parameters and experimental systems, and representative data (of both successful experiments and common problems). No background in SECCM is assumed and fundamentals are clearly explained at each stage with a rationale for the experimental steps provided. This work provides an entry point for the uninitiated to understand and use this powerful nanoscale electrochemical characterization technique.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"160-177"},"PeriodicalIF":4.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Fast Immunosensor Based on Biohybrid Self-Assembled Nanostructures for the Detection of KYNA as a Cerebrospinal Fluid Biomarker for Alzehimer’s Disease","authors":"A. Narváez*,&nbsp;J. Jiménez,&nbsp;M. Rodríguez-Núñez,&nbsp;M. Torre,&nbsp;E. Carro,&nbsp;M.-P. Marco and E. Domínguez,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0010210.1021/acsmeasuresciau.4c00102","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00102https://doi.org/10.1021/acsmeasuresciau.4c00102","url":null,"abstract":"<p >Although the role of kynurenic acid (KYNA) is not yet fully understood, recent research has implicated this tryptophan (Trp) metabolite as a significant biomarker in neurodegenerative diseases. In this study, we developed an immunosensor platform based on self-assembled polyelectrolyte multilayers (PEMs), employing an enzyme-labeled immunoreagent in a competitive displacement format that requires only a single wash step. This immunosensor enables the detection of KYNA and Trp with detection limits (LOD) of 9 pg/mL and 1.2 ng/mL, respectively. Results validated by traditional ELISA methods indicated elevated levels of KYNA and an increased KYNA/Trp ratio in the cerebrospinal fluid (CSF) of Alzheimer’s patients compared to controls, consistent with previous findings. Additionally, this immunosensor platform can be readily adapted to detect other neuroactive Trp metabolites by substituting specific immunoreagents, supporting a flexible profile-based approach. This platform could serve as a rapid, cost-effective clinical tool for monitoring neurological and psychiatric disorders, potentially advancing therapeutic strategy development.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"242–249 242–249"},"PeriodicalIF":4.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Fast Immunosensor Based on Biohybrid Self-Assembled Nanostructures for the Detection of KYNA as a Cerebrospinal Fluid Biomarker for Alzehimer's Disease.","authors":"A Narváez, J Jiménez, M Rodríguez-Núñez, M Torre, E Carro, M-P Marco, E Domínguez","doi":"10.1021/acsmeasuresciau.4c00102","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00102","url":null,"abstract":"<p><p>Although the role of kynurenic acid (KYNA) is not yet fully understood, recent research has implicated this tryptophan (Trp) metabolite as a significant biomarker in neurodegenerative diseases. In this study, we developed an immunosensor platform based on self-assembled polyelectrolyte multilayers (PEMs), employing an enzyme-labeled immunoreagent in a competitive displacement format that requires only a single wash step. This immunosensor enables the detection of KYNA and Trp with detection limits (LOD) of 9 pg/mL and 1.2 ng/mL, respectively. Results validated by traditional ELISA methods indicated elevated levels of KYNA and an increased KYNA/Trp ratio in the cerebrospinal fluid (CSF) of Alzheimer's patients compared to controls, consistent with previous findings. Additionally, this immunosensor platform can be readily adapted to detect other neuroactive Trp metabolites by substituting specific immunoreagents, supporting a flexible profile-based approach. This platform could serve as a rapid, cost-effective clinical tool for monitoring neurological and psychiatric disorders, potentially advancing therapeutic strategy development.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"242-249"},"PeriodicalIF":4.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Frequency Effective Capacitance <i>C</i> _ec and Membrane Resistance <i>Z</i> Readout for Solid-Contact Ion-Selective Electrodes.","authors":"Tingting Han, Sini Chen, Tao Song, Dongxue Han, Li Niu","doi":"10.1021/acsmeasuresciau.4c00093","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00093","url":null,"abstract":"<p><p>Here, we propose new single-frequency effective capacitance <i>C</i> _ec and membrane resistance <i>Z</i> readout principle for solid-contact ion-selective electrodes (SCISEs). Conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) doped with polystyrenesulfonate (PSS^-), <i>i.e.</i>, PEDOT(PSS), as solid contact and valinomycin-based membrane were prepared for K⁺-SCISEs. At high frequencies, the membrane resistance of K⁺-SCISEs corresponding to impedance absolute value <i>Z</i> was recorded constantly as KCl aqueous solution diluted with water. The membrane resistance <i>Z</i> increases as the electrolyte concentration decreases. Under identical dilution steps, the linear slope of the logarithmic membrane resistance log<i>Z</i> <i>vs</i> log<i>a</i> _K+ for K⁺-SCISEs with the spin-coated membrane is larger than that of the electrode covered with the drop-cast membrane. As the K⁺-SCISE resistance with the spin-coated membrane was reduced to hundreds of Ω, the log<i>Z</i> of K⁺-SCISEs is linearly proportional to log<i>a</i> _K+ in the range of -1 to -3.4, providing a possibility of utilizing membrane resistance <i>Z</i> as a calibration-free analytical signal for SCISEs. The effective capacitance <i>C</i> _ec of K⁺-SCISEs with the spin-coated membrane was performed in 0.1 M KCl applied with single frequency ranging from 1 MHz and decreases by a factor of 10 to 10 mHz. The obtained <i>C</i> _ec of K⁺-SCISEs with the spin-coated membrane is linearly proportional to log<i>f</i>in the range of 1 MHz to 10 Hz with a slope of <i>ca.</i> -0.97, while at a low frequency ranging from 1 Hz to 10 mHz, the linear slope of log<i>C</i> _ec <i>vs</i> log<i>f</i> is suppressed, where Warburg diffusion takes effect. Furthermore, the membrane resistance <i>Z</i> is independent of applied high frequencies, and the effective capacitance <i>C</i> _ec is independent of the excitation amplitude.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"216-225"},"PeriodicalIF":4.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Frequency Effective Capacitance Cec and Membrane Resistance Z Readout for Solid-Contact Ion-Selective Electrodes","authors":"Tingting Han*,&nbsp;Sini Chen,&nbsp;Tao Song,&nbsp;Dongxue Han and Li Niu,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0009310.1021/acsmeasuresciau.4c00093","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00093https://doi.org/10.1021/acsmeasuresciau.4c00093","url":null,"abstract":"<p >Here, we propose new single-frequency effective capacitance <i>C</i>_ec and membrane resistance <i>Z</i> readout principle for solid-contact ion-selective electrodes (SCISEs). Conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) doped with polystyrenesulfonate (PSS^–), <i>i.e.</i>, PEDOT(PSS), as solid contact and valinomycin-based membrane were prepared for K⁺-SCISEs. At high frequencies, the membrane resistance of K⁺-SCISEs corresponding to impedance absolute value <i>Z</i> was recorded constantly as KCl aqueous solution diluted with water. The membrane resistance <i>Z</i> increases as the electrolyte concentration decreases. Under identical dilution steps, the linear slope of the logarithmic membrane resistance log<i>Z</i> <i>vs</i> log<i>a</i>_K+ for K⁺-SCISEs with the spin-coated membrane is larger than that of the electrode covered with the drop-cast membrane. As the K⁺-SCISE resistance with the spin-coated membrane was reduced to hundreds of Ω, the log<i>Z</i> of K⁺-SCISEs is linearly proportional to log<i>a</i>_K+ in the range of −1 to −3.4, providing a possibility of utilizing membrane resistance <i>Z</i> as a calibration-free analytical signal for SCISEs. The effective capacitance <i>C</i>_ec of K⁺-SCISEs with the spin-coated membrane was performed in 0.1 M KCl applied with single frequency ranging from 1 MHz and decreases by a factor of 10 to 10 mHz. The obtained <i>C</i>_ec of K⁺-SCISEs with the spin-coated membrane is linearly proportional to log<i>f</i>in the range of 1 MHz to 10 Hz with a slope of <i>ca.</i> −0.97, while at a low frequency ranging from 1 Hz to 10 mHz, the linear slope of log<i>C</i>_ec <i>vs</i> log<i>f</i> is suppressed, where Warburg diffusion takes effect. Furthermore, the membrane resistance <i>Z</i> is independent of applied high frequencies, and the effective capacitance <i>C</i>_ec is independent of the excitation amplitude.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"216–225 216–225"},"PeriodicalIF":4.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Dual-Function Microelectronic Fibers for pH and Temperature Sensing: Toward In Vivo and Wearable Applications","authors":"Mahiro Kubo,&nbsp;Mayuko Abe,&nbsp;Etienne Le Bourdonnec,&nbsp;Sheau-Chyi Wu,&nbsp;To-En Hsu,&nbsp;Takao Inoue and Yuanyuan Guo*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0009210.1021/acsmeasuresciau.4c00092","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00092https://doi.org/10.1021/acsmeasuresciau.4c00092","url":null,"abstract":"<p >Temperature plays a crucial role in biological functions in normal physiological and pathological states and is intricately linked with chemical dynamics <i>in vivo</i> at the cellular, circuit, and system levels. Despite advances in temperature measurement technologies for internal monitoring, systems capable of simultaneously tracking localized temperature and chemical changes are still underdeveloped. In this study, we introduce dual-sensing hybrid fibers with a miniature footprint of &lt;400 μm in diameter, fabricated using the thermal drawing process. These fibers exhibit precise temperature sensitivity, detecting changes as small as 0.5 °C, and demonstrate highly sensitive and reversible pH detection, a critical physiological parameter. Furthermore, through laser micromachining and surface functionalization, we highlight the potential of these fibers for wearable applications in dual pH and temperature sensing. This innovative dual-sensing technology offers a versatile platform for probing temperature and chemical signaling <i>in vivo</i> and wearable applications, with significant implications for therapeutic development and a deeper understanding of biological processes in various environments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"208–215 208–215"},"PeriodicalIF":4.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Dual-Function Microelectronic Fibers for pH and Temperature Sensing: Toward <i>In Vivo</i> and Wearable Applications.","authors":"Mahiro Kubo, Mayuko Abe, Etienne Le Bourdonnec, Sheau-Chyi Wu, To-En Hsu, Takao Inoue, Yuanyuan Guo","doi":"10.1021/acsmeasuresciau.4c00092","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00092","url":null,"abstract":"<p><p>Temperature plays a crucial role in biological functions in normal physiological and pathological states and is intricately linked with chemical dynamics <i>in vivo</i> at the cellular, circuit, and system levels. Despite advances in temperature measurement technologies for internal monitoring, systems capable of simultaneously tracking localized temperature and chemical changes are still underdeveloped. In this study, we introduce dual-sensing hybrid fibers with a miniature footprint of <400 μm in diameter, fabricated using the thermal drawing process. These fibers exhibit precise temperature sensitivity, detecting changes as small as 0.5 °C, and demonstrate highly sensitive and reversible pH detection, a critical physiological parameter. Furthermore, through laser micromachining and surface functionalization, we highlight the potential of these fibers for wearable applications in dual pH and temperature sensing. This innovative dual-sensing technology offers a versatile platform for probing temperature and chemical signaling <i>in vivo</i> and wearable applications, with significant implications for therapeutic development and a deeper understanding of biological processes in various environments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"208-215"},"PeriodicalIF":4.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}