Romanian Review of Laboratory Medicine最新文献

{"title":"Serological and Clinical one year follow-up of Patients with Erythema migrans treated in a Romanian Infectious Disease Hospital","authors":"M. Lupse, V. Briciu, M. Flonta, V. Năstase, N. Todor, B. Kullberg","doi":"10.2478/rrlm-2014-0021","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0021","url":null,"abstract":"Abstract Erythema migrans (EM) is the most common and recognized clinical manifestation of early Lyme Borreliosis (LB) in Europe. Purpose: to evaluate clinically and serologically all the patients with EM and to correlate the serology with clinical approach. Material and method: A prospective analyses of all patients that came with EM in our University Hospital of Infectious Diseases from Cluj-Napoca Romania between 1st of April to 31st of August 2011. We registered data regarding: age, gender, interval between tick bite and EM occurrence, interval between EM appearance and hospital presentation, serology (IgM and IgG for Borrelia burgdorferi sl) at presentation and one year after, antibiotic treatment, duration of antibiotic treatment and clinical outcome. Results: 44 patients with EM, 2 had Multiple EM, 23 male (52 %), average age 41.3 years (min 3 years, max 84 years, median 43.5 years). Serology at presentation was positive in 15 patients for IgM and in 9 patients for IgG with ELISA and for 13 for IgM and 16 for IgG with WB. At the one year follow up positive results for IgM were found in 14 patients with ELISA and in 11 patients with WB. IgG was positive in 7 patients with ELISA and in 5 with WB. The average time interval between the tick bite and EM was not significant different for patients with negative or positive IgM or IgG. The interval between EM occurrence and treatment initiation had a strong significance for IgG. After one year no statistical significance was found for positive IgM for any of the analyzed factors while for IgG positivity we found a significant importance for age and duration of incubation. No influence of antibiotic or duration of treatment was found on serology of our patients. Conclusions: Our data brings additional evidence that the serological profile is unpredictable. Rezumat Eritemul migrator ( EM ) este cea mai comună și recunoscută manifestare clinică precoce a Borreliozei Lyme ( LB ) în Europa. Scop: de a evalua clinic și serologic toți pacienții cu EM și de a corela serologia cu abordarea clinică. Material si metodă: Un studiu prospectiv incluzand toți pacienții care au venit cu EM la Spitalul Clinic de Boli Infecțioase din Cluj - Napoca România între 1 aprilie și 31 august 2011. Am înregistrat date referitoare la: vârstă, sex, intervalul între înțepătura de căpușă și apariția EM, intervalul între apariția EM și prezentarea la spital, serologia (IgM și IgG pentru Borrelia burgdorferi sl) la prezentare și după un an, tratamentul cu antibiotice, durata tratamentului cu antibiotice și evolutia clinică. Rezultate: 44 pacienți cu EM, 2 au avut EM multiplu, 23 bărbați (52 %), cu vârsta medie de 41.3 ani (min 3 ani, maxim 84 ani, in medie 43.5 ani). Serologia la prezentare a fost pozitivă la 15 pacienți pentru IgM și la 9 pacienți pentru IgG cu ELISA și la 13 pentru IgM și la 16 pentru IgG cu WB. După un an de urmărire rezultate pozitive pentru IgM au fost găsite la 14 pacienți cu ELISA și la 11 de pacienți cu W","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115630538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of chromosomal imbalances using combined MLPA kits in patients with syndromic intellectual disability","authors":"A. Sireteanu, R. Popescu, E. Braha, C. Bujoran, L. Butnariu, L. Caba, Elena Graur, E. Gorduza, M. Grămescu, I. Ivanov, M. Panzaru, C. Rusu","doi":"10.2478/rrlm-2014-0019","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0019","url":null,"abstract":"Abstract Intellectual disability (ID) is a common disorder, with major consequences for individual, family and society. Due to clinical and genetic heterogeneity of ID, in about 50% of cases an etiologic diagnosis cannot be established. The aim of this study was to evaluate the ability of a combination of MLPA kits to establish the diagnosis in 369 patients with syndromic ID and normal or uncertain routine karyotype results. All patients were assessed for chromosome imbalance using SALSA MLPA P064 or P096 kits, if the phenotype was suggestive of a microdeletion syndrome (subgroup A - 186 patients), or subtelomeric P036 and P070 kits, if the phenotype was not suggestive of a microdeletion syndrome or if the result of the standard karyotype was uncertain (subgroup B - 183 patients). Abnormal results detected by these kits were further characterized using appropriate follow-up MLPA kits (Telomere Follow-up set, P029-A1, P250-B2, ME028-B1). In subgroup A we identified 25 patients with microdeletions (13.4%). Using subtelomere screening and follow-up kits in subgroup B we detected cryptic rearrangements in 7.5% cases and identified the origin of the unknown material noticed in the standard karyotype in 10 out of 11 patients. Summarizing data from the two groups, the combined use of MLPA kits led to the diagnosis in 10.6% (38/358) patients with normal karyotype. Using follow-up MLPA kits allowed us both to confirm abnormalities and to determine their size, which facilitated the interpretation of the clinical significance of these rearrangements. For laboratories that do not have yet access to microarray technology, using several MLPA kits represents an effective strategy for establishing the diagnosis in ID patients. Rezumat Dizabilitatea intelectuală (DI) este o afecțiune frecventă, cu consecințe majore pentru individ, familie și societate. Datorită heterogenității sale clinice și genetice, în aproximativ 50% din cazuri etiologia bolii nu poate fi stabilită. Scopul acestui studiu a fost evaluarea capacității de stabilire a diagnosticului etiologic la 369 pacienți cu DI sindromic și rezultat normal sau incert la cariotip folosind o combinație de kituri MLPA. Toţi pacienţii au fost investigaţi prin metoda MLPA, folosind fie kiturile SALSA MLPA P064 sau P096, dacă fenotipul a fost sugestiv pentru un sindrom cu microdeleţie (subgrupul A - 186 pacienți), fie kiturile subtelomerice P036 și P070, dacă fenotipul nu a fost sugestiv pentru un sindrom cu microdeleţie sau rezultatul la cariotipul standard a fost incert (subgrupul B - 183 pacienți). Rezultatele anormale detectate de aceste kituri au fost caracterizate folosind kiturile MLPA corespunzătoare de urmărire (Telomere Follow-up set, P029-A1, P250-B2, ME028-B1). În subgrupul A am identificat 25 de pacienți cu microdeleții (13,4%). Folosind kiturile de screening subtelomeric și de urmărire la subgrupul B am detectat rearanjări criptice în 7,5% din cazuri și am identificat originea materialului suplimentar ","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121275774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of the vitamin B12 status in an urban population sample from Romania: a pilot study","authors":"E. C. Crăciun, H. Colosi, V. Țărmure","doi":"10.2478/rrlm-2014-0018","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0018","url":null,"abstract":"Abstract The aim of this study was to evaluate the vitamin B12 status in a non-vegetarian sample of the adult urban population from Romania. The studied sample included 80 non vegetarian subjects aged between 19 and 74.6 years who did not use vitamin B12 supplements or vitamin B12 fortified food. The serum vitamin B12 assay was performed using electrochemiluminescence immunoassay on a Roche Elecsys 2010 analyzer. Among all subjects, 93.75% (95% CI 86.2-97.3%) had serum vitamin B12 concentrations in the reference range of the employed method (191-663 pg/ml). In these subjects, we found no statistically significant correlations between serum vitamin B12 concentration and age or between serum vitamin B12 concentration and hemoglobin level. The serum vitamin B12 levels did not differ significantly (p>0.05-Student’s t-test) between women and men. Subnormal serum levels of vitamin B12 were observed in 5% (95%CI 1.96-12.16%) of the investigated subjects, associated with irritable bowel syndrome and with long-term therapy with proton pomp inhibitors or birth control pills. In the present study, we have identified a large number of subjects with marginal depletion of vitamin B12 in the population aged below 50 years. These results suggest the need of a screening for vitamin B12 status in people from all age groups, the importance of the identification of responsible causes for the high prevalence of vitamin B12 marginal status, as well as the fact that monitoring the vitamin B12 status is especially important in subjects undergoing long-term treatment with certain drugs Rezumat Scopul acestui studiu a fost evaluarea statusului vitaminei B12 într-un eşantion al populaţiei urbane adulte din România. În studiu au fost incluşi 80 de subiecţi non-vegetarieni, cu vârste cuprinse între 19 şi 74,6 ani, care nu au utilizat suplimente de vitamina B12 sau alimente fortificate cu vitamina B12. Dozarea vitaminei B12 serice s-a realizat prin metoda imunochimică cu detecţie prin chemiluminiscenţă pe analizor Elecsys 2010 (Roche). Concentraţia serică a vitaminei B12 s-a încadrat în intervalul de referinţă (191-663 pg/ml) la 93,75% (95% CI 86,2-97,3%) din subiecţi. La aceşti subiecţi nu s-au evidenţiat corelaţii seminificative statistic între concentraţia serică a vitaminei B12 şi vârsta subiecţilor sau concentraţia hemoglobinei. Nu s-au găsit nici diferenţe semnificative (p>0.05) ale statusului vitaminic la femei faţă de bărbaţi. Concentraţia serică a vitaminei B12 s-a situat sub limita inferioară a intervalului de referinţă la 5% (95%CI 1,96-12,16%) din subiecţii investigaţi, fiind asociată tratamentului cu contraceptive orale, inhibitori ai pompei de protoni (IPP) respectiv sindromului de colon iritabil. La un număr mare de subiecţi cu vârsta sub 50 de ani, concentraţia serică a vitaminei B12 s-a încadrat în limitele corespunzătoare statusului marginal. Rezultatele studiului sugerează necesitatea efectuării screeningului statusului vitaminei B12 la întreaga populaţie, ","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121789257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlations between vascular invasion, neural structures invasion and microvessel density with clinicopathological parameters in gastric cancer","authors":"A. Bădescu, C. Georgescu, C. Mărgăritescu, D. Cernea, M. Bălășoiu, Ş. Crăiţoiu, C. C. Georgescu","doi":"10.2478/rrlm-2014-0025","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0025","url":null,"abstract":"Abstract Aim: Our objective in conducting this study was to estimate the presence of lymphatic, blood vessel and neural invasion on Hematoxylin & Eosin (H&E) staining and also, the microvessel density detected by immunohistochemistry (MVD), in gastric adenocarcinoma, as well as their relationship with the clinical, pathological and biological characteristics of the tumors. Materials and Methods: To assess the vascular and neural invasion in our study, we included 367 patients diagnosed with gastric cancer. For the immunohistochemical study of MVD, from all cases with gastric carcinoma, we selected 28 patients, 12 patients with gastric biopsy and 16 patients with total gastric resection, which established the TNM stage. All the gastric biopsies and surgical samples were prepared using the paraffin-embedding method and H&E staining and using anti-CD31 and anti-CD34 antibodies for the assessment of intratumoral MVD. Results: The positive blood vessel invasion was associated in a significant way with advanced stages (p <0.01) and high grade carcinomas (p<0.01), while lymphatic invasion was very significant associated only with advanced stage tumors (p < 0.001). Regarding peri- and intraneural invasion, there was a significant matching with the female gender (p < 0.05), advanced stages of disease (p < 0.001), the diffuse type of gastric carcinoma (p <0.05), and with poorly differentiated tumors (p<0.05). There was a close relationship between CD34 MVD and the diffuse type of gastric carcinomas, according to Lauren’s classification (p<0.05), and poorly differentiated tumors (p<0.05). The CD34 MVD values mean was significant correlated with TNM stage, especially III and IV stages (p<0.01). Conclusions: Blood vessel invasion, neural invasion and CD34 MVD play a significant role in tumors biological behavior and they can be used as important prognostic parameters which describes the aggressiveness of gastric carcinomas. Rezumat Scopul: Scopul studiului efectuat a fost acela de a estima prezenţa invaziei tumorale la nivelul vaselor limfatice, sanguine şi la nivel neural în carcinoamele gastrice pe preparatele colorate hematoxilină-eozină (H-E) şi, de asemenea, densitatea microvascularizatiei tumorale (MVD), detectată imunohistochimic, precum şi relaţia acestora cu parametrii clinico-patologici şi biologici ai tumorilor. Material şi metodă. Pentru evaluarea invaziei limfo-vasculare şi neurale am inclus în studiu 367 de pacienţi diagnosticaţi cu carcinoame gastrice. Pentru studiul imunohistochimic al MVD, au fost selectaţi 28 de pacienţi, din care 16 pacienţi cu gastrectomomie totală, în urma căreia s-a stabilit stadiul TNM al tumorii primare şi 12 pacienţi cu biopsie gastrică. Biopsiile gastrice şi probele chirurgicale au fost procesate folosind tehnica de includere la parafină şi coloraţia hematoxilină-eozină, iar pentru evaluarea imunohistochimică a MVD s-au utilizat anticorpii anti-CD31 şi anti-CD34. Rezultate: Prezenţa invaziei tumorale la nivelul vasel","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125200289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helicobacter pylori infection in HIV-positive patients with digestive complaints","authors":"Nina Șincu, L. Chiriac, M. Fodor, A. Incze, S. Bățagă","doi":"10.2478/rrlm-2014-0022","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0022","url":null,"abstract":"Abstract Background. Patients infected with human immunodeficiency virus (HIV), especially at advanced stages of HIV infection and low CD4+ T-lymphocytes levels, were reported to be less frequently co-infected with Helicobacter pylori than general population, according to literature data. Purpose: to study Helicobacter pylori infection in HIV-positive hosts with digestive complaints. Methods: retrospective, analytical, case-control study (November 2011 - December 2013), upon two groups of patients with gastro-intestinal symptoms tested for Helicobacter pylori stool antigen at the Infectious Diseases Laboratory, Clinical County Hospital Mureş. Group A included 44 HIV-positive patients, group B: 58 HIV-negative subjects. We first compared groups A and B regarding the frequency of Helicobacter pylori infection. Group A was afterwards divided into two sub-groups, according to the status of Helicobacter pylori infection: group A1: 5 Helicobacter pylori-positive subjects, group A2: 39 Helicobacter pylori-negative ones. We collected information regarding CD4+ T-lymphocytes level, HIV-RNA plasma viral load, previous antibiotic and antiretroviral therapy, co-morbidities, comparing A1 and A2 subgroups. Data were processed using GraphPad Prism 5 programme. Results. The frequency of Helicobacter pylori infection was 11.36% among HIV-positive patients and 13.79% in HIV-negative ones, without statistically significant difference. We found no statistically significant differences between subgroups A1 and A2 regarding CD4+ T-lymphocytes level, HIV-RNA plasma viral load, antibiotic / antiretroviral therapy. Conclusions. Though Helicobacter pylori infection may represent one of the causes of gastro-intestinal symptoms in HIV-positive patients, its frequency did not differ to that registered in the general population, in our study. Rezumat Introducere. Datele din literatura de specialitate raportează o frecvenţă mai scăzută a infecţiei cu Helicobacter pylori în rândul pacienţilor infectaţi cu virusul imunodeficienţei umane (HIV), în special la nivele scăzute ale limfocitelor T CD4+ sau în stadii avansate ale infecţiei HIV. Scopul lucrării: studiul infecţiei cu Helicobacter pylori la pacienţii seropozitivi HIV cu acuze digestive. Material si metode: studiu retrospectiv, analitic, cazcontrol (noiembrie 2011 - decembrie 2013), pe două loturi de pacienţi cu simptome gastro-intestinale, la care s-a determinat coproantigenul Helicobacter pylori la Laboratorul Boli Infecţioase, Spitalul Clinic Judeţean Mureş. Grupul A includea 44 pacienţi HIV-pozitivi, grupul B 58 subiecţi HIV-negativi. După ce am comparat frecvenţa infecţiei cu Helicobacter pylori în grupurile A şi B, am împărţit grupul A în două subgrupuri: A1, alcătuit din 5 pacienţi Helicobacter pylori-pozitivi şi A2, format din 39 pacienţi Helicobacter pylori-negativi, pe care le-am comparat în privinţa nivelului limfocitelor T CD4+, încărcăturii virale plasmatice HIV-ARN, terapiei antibiotice / antiretrovirale. Anal","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125975487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascular Endothelial Growth Factor Insertion/Deletion gene polymorphism in patients with type 2 diabetes and diabetic peripheral polyneuropathy","authors":"A. Stoian, A. Bacârea, Anca Moţăţăianu, M. Stoian, F. Gliga, V. Bacârea, C. Duicu, C. Bănescu","doi":"10.2478/rrlm-2014-0023","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0023","url":null,"abstract":"Abstract The aim of this work was to study for the first time in Romania Insertion/Deletion (I/D) polymorphism of the Vascular Endothelial Growth Factor (VEGF) gene in a group of patients with established type 2 diabetes mellitus (DM) and diabetic peripheral neuropathy (DPN) compared with a control group. This was a case-control study consisting of a group of 84 patients with type 2 DM and DPN, diagnosed by clinical neurological examination and electrophysiological nerve conduction studies and a control group of 90 healthy volunteers. For deoxyribonucleic acid (DNA) isolation, a DNA purification kit from Zymo Research was used. In vitro amplification of DNA sequences was achieved by polymerase chain reaction (PCR). Selective in vitro amplification of a DNA fragment of known sequence is based on the principle of extension of a primer (“primer and PCR amplicon”). DNA fragments were separated by gel electrophoresis. For proper viewing and interpreting of agarose gels Vilber Lourmat system was used. D allele frequency of VEGF was significantly higher in patients with diabetic peripheral neuropathy (53.57%) compared with controls (25%), p=0.0001. There is a positive association between I/D polymorphism of VEGF gene and the presence of diabetic peripheral polyneuropathy. Our study suggests that D allele of VEGF gene is a risk factor for the occurrence of DPN. Rezumat Scopul acestei lucrări constă în studierea pentru prima dată în România a polimorfismului I/D al genei VEGF la un grup de pacienți cu diabet zaharat tip 2 și polineuropatie diabetică periferică comparativ cu un grup de control. Acesta este un studiu de tip caz-control constând din 84 de pacienți cu diabet zaharat tip 2 și polineuropatie diabetică periferică, diagnosticată prin examen clinic neurologic și studii electrofiziologice de conducere nervoasă și un grup de control alcătuit din 94 de voluntari sănătoși. Pentru izolarea acidului dezoxiribonucleic s-a utilizat kitul ADN de la Zymo Research. Amplificarea in vitro a secvențelor de ADN a avut loc prin reacția de polimerizare în lanț. Amplificarea selectivă in vitro a unui fragment de ADN cu secvenţă cunoscută se realizează pe baza principiului extensiei unei amorse (“primer sau amplimer”). Fragmentele de ADN au fost separate prin electroforeză în gel. Vizualizarea și interpretarea gelurilor de agaroza s-a efectuat cu ajutorul sistemului de fotodocumentare de la Vilber Lourmat. Frecvența alelei D a VEGF a fost semnificativ statistic mai mare la pacienţii cu neuropatie periferică diabetică (53.57%) comparativ cu grupul de control (25%). Există o asociere pozitivă între polimorfismul I/D al genei VEGF și prezența polineuropatiei diabetice periferice. Studiul nostru sugerează că alela D a genei VEGF este un factor de risc pentru apariția polineuropatiei diabetice periferice.","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130596571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of platelet aggregation and platelet derived microparticles in acute leukemia patients","authors":"Andrada Costache, S. Angelescu, D. Barbu, E. Popescu, D. M. Mut Popescu, A. Lupu","doi":"10.2478/rrlm-2014-0015","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0015","url":null,"abstract":"Abstract Patients with acute leukemia develop abnormalities of haemostasis, leading not only to bleeding, but also to thrombotic complications. The pathogenesis of these complications is complex and multifactorial. Because platelets and platelet derived microparticles are key players in haemostasis and thrombosis, we presumed their roles in the prediction of bleeding and thrombotic complications. Our study groups included 24 patients with acute leukemia and 16 healthy volunteers. Platelet aggregation evaluation was performed by impedance whole blood aggregometry and the ennumeration of platelet derived microparticles was done by means of flow cytometry. Eight patints developed hemorrhagic complications associated with reduced platelet aggregation response at hospital admission. Major thrombotic events occurred in 5 patients, being preceded by increased platelet aggregation in 3 cases and high level of platelet derived microparticles in 2 cases. Our findings reveal that whole blood platelet aggregometry could be a valuable tool especially in the detection of platelet hyperreactivity and in the prediction of thrombotic events. A high level of platelet derived microparticles could also predict thrombosis. These hypotheses need further evaluation and confirmation on larger number of patients. Rezumat Pacienţii cu leucemie acută dezvoltă anomalii ale hemostazei care determină apariţia de complicaţii hemoragipare şi chiar trombotice. Patogeneza acestor complicaţii este complexă si multifactorială. Deoarece trombocitele şi microparticulele trombocitare reprezintă elemente cheie în cadrul proceselor de hemostază si tromboză, am încercat să evidenţiem rolul lor în predicţia complicaţiilor hemoragice şi trombotice. Grupurile studiate au inclus 24 de pacienţi cu leucemie acută şi 16 voluntari sănătoşi. Evaluarea agregării plachetare s-a realizat prin agregometrie prin impedanţa în sânge integral, iar pentru determinarea microparticulelor trombocitare s-a utilizat citometria în flux. Opt pacienţi au dezvoltat complicaţii hemoragipare, care au fost asociate cu agregabilitate trombocitară diminuată la internare. Evenimentele trombotice majore au complicat evoluţia a 5 pacienţi şi au fost precedate de agregare trombocitară crescută la 3 cazuri şi nivel crescut de microparticule trombocitare la 2 cazuri. Rezultatele noastre evidenţiaza faptul că agregometria plachetară în sânge integral poate fi un instrument valoros în special în identificarea hiperreactivitaţii plachetare şi în predicţia evenimentelor trombotice. Un nivel crescut de microparticule trombocitare poate, de asemenea, să anticipeze apariţia trombozei. Aceste ipoteze necesită evaluare si confirmare pe un număr mai mare de pacienţi.","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133484514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological, clinical and epidemiological aspects of Lyme borreliosis in Mures County, Romania","authors":"Brîndușa Țilea, Grațiela Tripon, S. Voidăzan, I. Tilea","doi":"10.2478/rrlm-2014-0020","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0020","url":null,"abstract":"Abstract Lyme borreliosis (LB) is the most frequent infection transmitted by ticks from the Ixodes genus, both in the United States of America (USA), and in Europe. The objectives of this study were the monitoring of the incidence and clinical manifestations of the disease in Mures County, Romania. Material and method. The study was conducted over a period of two years, January 1st 2010 -December 31st 2011, on a number of 120 consecutive patients. The definite or probable diagnosis of LB was established on the Centers for Disease Control and Prevention (CDC, Atlanta, USA) criteria, and on the European Union Concerted Action on Lyme borreliosis (EUCALB). ELISA and Western-Blot techniques were used for identification of IgM and IgG antiBb from the serum and CSF. Results. There were 44 cases in 2010 and 76 cases in 2011. According to the case definition, 106 cases were confirmed as LB and 14 were probable. LB was more frequently diagnosed in children, young adults and adults, compared to the over 60 years old age group. The incidence of disease was higher in women patients, 68 (56.66%) compared to men - 52 patients (43.33%), with a higher proportion in people coming from an urban setting, 78 patients (65%), compared to people coming from rural settings 42 patients (35%). Clinical manifestations were acute, like erythema migrans (EM) in 64.16% of the patients, neurological in 22.5% of the patients, joint related in 1.66% of the patients and cardiac in 0.83% of the cases. Conclusions. In Mures County we have observed an increased incidence of this disease in 2011 vs. 2010. The predominant clinical manifestations were the acute dermatological and neurological ones. Rezumat Borrelioza Lyme (BL) este cea mai frecventă infecţie transmisă de căpuşe din genul Ixodes, atât în Statele Unite ale Americii (SUA), cât şi în Europa. Obiectivele studiului au constat în monitorizarea incidenţei şi a manifestărilor clinice ale bolii în judeţul Mureș. Material şi metodă. Studiul s-a efectuat pe o perioadă de 2 ani, 1 ianuarie 2010 - 31 decembrie 2011, pe un număr de 120 pacienţi. Diagnosticul cert sau probabil al BL s-a stabilit pe baza criteriilorCenters for Diseases Control and Prevention (CDC, Atlanta, SUA) şi European Union Concerted Action on Lyme Borreliosis (EUCALB) şi anume epidemiologice, clinice şi serologice. Pentru identificarea anticorpilor antiBb IgM, IgG din ser şi LCR s-au utilizat tehnicile ELISA şi Western-Blot. Rezultate. În anul 2010 s-au înregistrat 44 cazuri, iar în anul 2011, 76 cazuri. Conform definiţiei de caz, 106 cazuri au fost confirmate, 14 probabile. BL a fost evidenţiată cu o frecvenţă mai ridicată la copii, adulţi tineri şi adulţi, comparativ cu grupa de vârstă peste 60 ani. Incidenţa afecţiunii a fost mai ridicată la sexul feminin, 68 pacienţi (56,66%) faţă de sexul masculin 52 pacienţi (43,33%), cu o pondere, mai crescută la persoanele din mediul urban, 78 pacienţi (65,0%), comparativ cu cele din mediul rural 42 pacienţi (35,0%). Mani","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128574966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Prostate-Specific Antigen in Prostate Cancer Screening","authors":"T. Constantin, V. Mădan, M. Constantin, S. Morariu, B. Braticevici","doi":"10.2478/rrlm-2014-0024","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0024","url":null,"abstract":"Abstract Prostate cancer is, after lung cancer, the most common malignant disease diagnosed in the male population. The introduction into the practice used during the 80’s and 90’s of the determination of serum Prostate-Specific Antigen (PSA) levels, as a component of screening for prostate cancer, was a turning point in the medical practice. Due to this enzyme produced exclusively by the prostate gland, the prostate cancer detection rate (in curative, intracapsular stages) improved significantly. Serum PSA is a better predictive factor for prostate cancer (PC) than digital rectal examination or transrectal prostatic ultrasound. Rezumat Cancerul de prostată reprezintă, după cancerul pulmonar, cea mai frecventă afecţiune malignă diagnosticată în cadrul populaţiei masculine. Introducerea în practica curentă în anii 80-90 a determinării Antigenului Specific al Prostatei (PSA) seric, ca şi componentă a screeningului pentru cancerul de prostată, a reprezentat un moment de răscruce în practica medicală. Datorită acestei enzime produsă exclusiv de către glanda prostatică, rata detecţiei cancerului de prostată (în stadii curative, intracapsulare) s-a îmbunătăţit semnificativ. PSA - ul seric reprezintă un factor predictiv pentru cancerul de prostată (CP) mai bun decât tuşeul rectal sau ecografia prostatică transrectală","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125961435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-anticoagulant and EDTA dependent pseudothrombocytopenia. Case reports on two pediatric patients","authors":"S. Arghirescu, M. Bataneant, C. Jinca, A. Pașcalău, M. Lelik, Mihaela Preja, L. Ritli, E.C. Ursu, M. Serban, H. Ioniță","doi":"10.2478/rrlm-2014-0017","DOIUrl":"https://doi.org/10.2478/rrlm-2014-0017","url":null,"abstract":"Abstract Pseudothrombocytopenia is an in vitro sampling problem which may mislead the diagnosis towards the more critical condition of thrombocytopenia. The phenomenon occurs when the anticoagulant used while testing the blood sample causes clumping of platelets which mimics low platelet count without any clinical signs. This may determine unnecessary, expensive and invasive investigations and even treatment. In this article we report two cases of pseudothrombocytopenia diagnosed in pediatric patients. Rezumat Pseudotrombocitopenia este o anomalie in vitro legată de recoltarea probei de sânge, care poate conduce în mod eronat la diagnosticarea unei afecțiuni mai grave și anume trombocitopenia. Acest fenomen apare atunci când anticoagulantul folosit pentru recoltarea probelor de sânge în vederea testării acestora cauzează agregarea trombocitară, mimând astfel un număr scăzut de trombocite fără ca pacientul să prezinte semnele clinice ale unei trombocitopenii. Această anomalie poate determina efectuarea unor investigatii inutile, costisitoare și invazive, și chiar instituirea unui tratament nejustificat. În acest articol prezentăm două cazuri de pseudotrombocitopenie diagnosticată la pacienți pediatrici.","PeriodicalId":288498,"journal":{"name":"Romanian Review of Laboratory Medicine","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131876792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}