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Application of quantum dots CdZnSeS / ZnS luminescence, enhanced by plasmons of silver rough surface for detection of albumin in blood facies of infected person 应用银粗糙表面等离子体增强量子点CdZnSeS / ZnS发光检测感染者血相白蛋白
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2299718
E. Konstantinova, A. Zyubin, E. Moiseeva, K. Matveeva, V. Slezhkin, I. Samusev, V. Bryukhanov
{"title":"Application of quantum dots CdZnSeS / ZnS luminescence, enhanced by plasmons of silver rough surface for detection of albumin in blood facies of infected person","authors":"E. Konstantinova, A. Zyubin, E. Moiseeva, K. Matveeva, V. Slezhkin, I. Samusev, V. Bryukhanov","doi":"10.1117/12.2299718","DOIUrl":"https://doi.org/10.1117/12.2299718","url":null,"abstract":"The study of the luminescence of CdZnSeS / ZnS quantum dots (QDs) absorbed on the rough surface of a silver film, including the energy transfer between human serum albumin molecules, isolated from the blood plasma of healthy and infected with sepsis patients, was performed by spectral-kinetic methods.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124808870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Cavity ring down spectrometry for disease diagnostics using exhaled air 用呼出的空气进行疾病诊断的腔环衰谱法
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297126
G. Revalde, K. Grundsteins, J. Alnis, A. Skudra
{"title":"Cavity ring down spectrometry for disease diagnostics using exhaled air","authors":"G. Revalde, K. Grundsteins, J. Alnis, A. Skudra","doi":"10.1117/12.2297126","DOIUrl":"https://doi.org/10.1117/12.2297126","url":null,"abstract":"In this paper we report the current stage of the development of a cavity ring-down spectrometer (CRDS) system using exhaled human breath analysis for the diagnostics of different diseases like diabetes and later lung cancer. The portable CRDS system is made in ultraviolet spectral region using Nd:Yag laser 266 nm pulsed light. Calibration of the CRDS system was performed using generated samples by KinTek automated permeation tube system and self-prepared mixtures with known concentration of benzene and acetone in air. First experiments showed that the limits of detection for benzene and acetone are several tens of ppb.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115320344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Development of optical WGM resonators for biosensors 生物传感器用光学WGM谐振器的研制
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297551
I. Brice, A. Pirktina, A. Ubele, K. Grundsteins, A. Atvars, R. Viter, J. Alnis
{"title":"Development of optical WGM resonators for biosensors","authors":"I. Brice, A. Pirktina, A. Ubele, K. Grundsteins, A. Atvars, R. Viter, J. Alnis","doi":"10.1117/12.2297551","DOIUrl":"https://doi.org/10.1117/12.2297551","url":null,"abstract":"Whispering Gallery Mode (WGM) resonators are very sensitive to nanoparticles attaching to the surface. We simulate this process using COMSOL Wave Optics module. Our spherical WGM resonators are produced by melting a tip of an optical fiber and we measure optical Q factors in the 105 range. Molecular oxygen lines of the air in the 760 nm region are used as reference markers when looking for the shifts of the WGM resonance lines. We demonstrate WGM microresonator surface coating with a layer of ZnO nanorods as well as with polystyrene microspheres. Coatings produce increased contact surface. Additional layer of antigens/antibodies will be coated to make high-specificity biosensors.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"50 403 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115516830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Image quality enhancement for skin cancer optical diagnostics 用于皮肤癌光学诊断的图像质量增强
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297579
D. Bļizņuks, I. Kuzmina, K. Boločko, A. Lihachev
{"title":"Image quality enhancement for skin cancer optical diagnostics","authors":"D. Bļizņuks, I. Kuzmina, K. Boločko, A. Lihachev","doi":"10.1117/12.2297579","DOIUrl":"https://doi.org/10.1117/12.2297579","url":null,"abstract":"The research presents image quality analysis and enhancement proposals in biophotonic area. The sources of image problems are reviewed and analyzed. The problems with most impact in biophotonic area are analyzed in terms of specific biophotonic task – skin cancer diagnostics. The results point out that main problem for skin cancer analysis is the skin illumination problems. Since it is often not possible to prevent illumination problems, the paper proposes image post processing algorithm – low frequency filtering. Practical results show diagnostic results improvement after using proposed filter. Along that, filter do not reduces diagnostic results’ quality for images without illumination defects. Current filtering algorithm requires empirical tuning of filter parameters. Further work needed to test the algorithm in other biophotonic applications and propose automatic filter parameter selection.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124529712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Implicit dosimetry of microorganism photodynamic inactivation 微生物光动力失活的隐式剂量学
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297221
M. Tamošiūnas, Neringa Kuliešienė, R. Daugelavičius
{"title":"Implicit dosimetry of microorganism photodynamic inactivation","authors":"M. Tamošiūnas, Neringa Kuliešienė, R. Daugelavičius","doi":"10.1117/12.2297221","DOIUrl":"https://doi.org/10.1117/12.2297221","url":null,"abstract":"Photosensitization based antibacterial treatment is efficient against a broad range of pathogens but it utilizes suboptimal dosimetry with an explicit (and very broad range) determination of sensitizer concentration, light dose and fluence rates. In this study we verified the implicit dosimetry approach for pathogen photodynamic treatment, employing protoporphyrin IX (ppIX) photobleaching to assess the killing efficacy against Staphylococcus aureus and Candida albicans cells. The results show that there was an increased kill of S. aureus and C. albicans at higher degree of ppIX fluorescence decay. Therefore ppIX photobleaching can be incorporated into the PDI dose metric offering to predict the pathogen killing efficacy during photodynamic treatment.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115561378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
AOTF-based optical system of a microscope module for multispectral imaging techniques 基于aotf光学系统的显微镜模块多光谱成像技术
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297614
O. Polschikova, A. Machikhin, V. Batshev, A. Ramazanova, A. Belov, V. Pozhar
{"title":"AOTF-based optical system of a microscope module for multispectral imaging techniques","authors":"O. Polschikova, A. Machikhin, V. Batshev, A. Ramazanova, A. Belov, V. Pozhar","doi":"10.1117/12.2297614","DOIUrl":"https://doi.org/10.1117/12.2297614","url":null,"abstract":"Multi-spectral imaging techniques are widely used in microscopy for many applications. One of the most widespread spectral elements for this purpose is an imaging acousto-optic tunable filter (AOTF). AOTF-based contrast visualization is especially effective when used together with other imaging techniques. Simultaneous utilization of two or more imagers requires optical coupling to provide point-to-point matching of the obtained images. Small linear and angular aperture of AOTF additionally hampers the development of multi-sensor imaging systems for microscopy. In this paper, we present a compact optical system which allows to integrate AOTF-based spectral imager into the schemes of conventional microscopes and provide high-quality spectral image of the same scale as in another, for example, wideband channel. The effectiveness of the proposed approach is demonstrated experimentally.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132248836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Color constancy in dermatoscopy with smartphone 用智能手机进行皮肤镜检查时的颜色稳定性
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297252
B. Cugmas, F. Pernus, B. Likar
{"title":"Color constancy in dermatoscopy with smartphone","authors":"B. Cugmas, F. Pernus, B. Likar","doi":"10.1117/12.2297252","DOIUrl":"https://doi.org/10.1117/12.2297252","url":null,"abstract":"The recent spread of cheap dermatoscopes for smartphones can empower patients to acquire images of skin lesions on their own and send them to dermatologists. Since images are acquired by different smartphone cameras under unique illumination conditions, the variability in colors is expected. Therefore, the mobile dermatoscopic systems should be calibrated in order to ensure the color constancy in skin images. In this study, we have tested a dermatoscope DermLite DL1 basic, attached to Samsung Galaxy S4 smartphone. Under the controlled conditions, jpeg images of standard color patches were acquired and a model between an unknown device-dependent RGB and a deviceindependent Lab color space has been built. Results showed that median and the best color error was 7.77 and 3.94, respectively. Results are in the range of a human eye detection capability (color error ≈ 4) and video and printing industry standards (color error is expected to be between 5 and 6). It can be concluded that a calibrated smartphone dermatoscope can provide sufficient color constancy and can serve as an interesting opportunity to bring dermatologists closer to the patients.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134510173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Application of fluorescent and vibration spectroscopy for septic serum human albumin structure deformation during pathology 荧光和振动光谱在脓毒症患者病理过程中血清白蛋白结构变形的应用
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2295315
A. Zyubin, E. Konstantinova, V. Slezhkin, K. Matveeva, I. Samusev, V. Bryukhanov
{"title":"Application of fluorescent and vibration spectroscopy for septic serum human albumin structure deformation during pathology","authors":"A. Zyubin, E. Konstantinova, V. Slezhkin, K. Matveeva, I. Samusev, V. Bryukhanov","doi":"10.1117/12.2295315","DOIUrl":"https://doi.org/10.1117/12.2295315","url":null,"abstract":"In this paper we perform results of conformational analysis of septic human serum albumin (HSA) carried out by Raman spectroscopy (RS), infrared (IR) spectroscopy and fluorescent spectroscopy. The main vibrational groups were identified and analyzed for septic HSA and its health control. Comparison between Raman and IR results were done. Fluorescent spectral changes of Trp-214 group were analyzed. Application of Raman, IR spectroscopy, fluorescent spectroscopy for conformational changes study of HSA during pathology were shown.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132780888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First results of cavity ring down signals from exhaled air 空腔的第一个结果是从呼出的空气中提取信号
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297277
G. Revalde, K. Grundsteins, J. Alnis, A. Skudra
{"title":"First results of cavity ring down signals from exhaled air","authors":"G. Revalde, K. Grundsteins, J. Alnis, A. Skudra","doi":"10.1117/12.2297277","DOIUrl":"https://doi.org/10.1117/12.2297277","url":null,"abstract":"In this paper we report first results from the developed cavity ring-down spectrometer for application in human breath analysis for the diagnostics of diabetes and later for early detection of lung cancer. Our cavity ring-down spectrometer works in UV region with pulsed Nd:YAG laser at 266 nm wavelength. First experiments allow us to determine acetone and benzene at the level bellow ppm. In our experiment, first results from breath samples from volunteers after doing different activities were collected and examined. Influence of the smoking on the breath signals also was examined.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"09 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127443754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Evaluation of nitroglycerin effect on remote photoplethysmogram waveform acquired at green and near infra-red illumination 评价硝酸甘油对绿色和近红外光照下远距光容积图波形的影响
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297385
Z. Marcinkevics, U. Rubins, A. Caica, A. Grabovskis
{"title":"Evaluation of nitroglycerin effect on remote photoplethysmogram waveform acquired at green and near infra-red illumination","authors":"Z. Marcinkevics, U. Rubins, A. Caica, A. Grabovskis","doi":"10.1117/12.2297385","DOIUrl":"https://doi.org/10.1117/12.2297385","url":null,"abstract":"Assessment of skin microcirculation provides diagnostically valuable information during the early stages of pathologies. The simple, cost-effective and intrusive alternative to existing circulation assessment methods is remote photoplethysmography (rPPG). The objective of the present pilot study was to reveal an effect on sublingual administration of 1 mg nitroglycerin on systemic hemodynamic parameters and rPPG waveforms, at 810 nm and 530nm illumination. The protocol comprised 3 minutes of baseline recording, 15 minutes recording of NTG effect, 2 minutes of arterial occlusion and the following 3 min reactive hyperemia. Two PPG signals were acquired from glabrous skin of the middle finger distal phalange, consecutively at 530 nm and 810nm, 125 fps per channel, and systemic cardiovascular parameters were continuously registered in a beat-to-beat manner with a Finameter-midi system. The NTG effect was observed 0.7- 1.2 minutes post administration, reaching its maximum after 3 minutes. Systemic cardiovascular parameters significantly changed: mean arterial pressure decreased by 7.7±3.6%, total peripheral resistance by 10.5±9.0%, whereas the heart rate increased by 27.2±11.8%. Substantial alterations were observed for rPPG waveforms during NTG effect, decreasing reflection and stiffness indices. It has been concluded that rPPG waveform may provide information related to arterial stiffness, and could be potentially utilized in the clinics.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126720917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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