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Computed aided system for separation and classification of the abnormal erythrocytes in human blood 人血液中异常红细胞分离与分类的计算机辅助系统
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297218
M. Wąsowicz, M. Grochowski, M. Kulka, Agnieszka Mikołajczyk, M. Ficek, K. Karpienko, M. Cićkiewicz
{"title":"Computed aided system for separation and classification of the abnormal erythrocytes in human blood","authors":"M. Wąsowicz, M. Grochowski, M. Kulka, Agnieszka Mikołajczyk, M. Ficek, K. Karpienko, M. Cićkiewicz","doi":"10.1117/12.2297218","DOIUrl":"https://doi.org/10.1117/12.2297218","url":null,"abstract":"The human peripheral blood consists of cells (red cells, white cells, and platelets) suspended in plasma. In the following research the team assessed an influence of nanodiamond particles on blood elements over various periods of time. The material used in the study consisted of samples taken from ten healthy humans of various age, different blood types and both sexes. The markings were leaded by adding to the blood unmodified diamonds and oxidation modified. The blood was put under an impact of two diamond concentrations: 20μl and 100μl. The amount of abnormal cells increased with time. The percentage of echinocytes as a result of interaction with nanodiamonds in various time intervals for individual specimens was scarce. The impact of the two diamond types had no clinical importance on red blood cells. It is supposed that as a result of longlasting exposure a dehydratation of red cells takes place, because of the function of the cells. The analysis of an influence of nanodiamond particles on blood elements was supported by computer system designed for automatic counting and classification of the Red Blood Cells (RBC). The system utilizes advanced image processing methods for RBCs separation and counting and Eigenfaces method coupled with the neural networks for RBCs classification into normal and abnormal cells purposes.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"167 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127622048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Rosacea assessment by erythema index and principal component analysis segmentation maps 用红斑指数和主成分分析分割图评价酒渣鼻
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2285175
I. Kuzmina, U. Rubins, I. Saknite, J. Spigulis
{"title":"Rosacea assessment by erythema index and principal component analysis segmentation maps","authors":"I. Kuzmina, U. Rubins, I. Saknite, J. Spigulis","doi":"10.1117/12.2285175","DOIUrl":"https://doi.org/10.1117/12.2285175","url":null,"abstract":"RGB images of rosacea were analyzed using segmentation maps of principal component analysis (PCA) and erythema index (EI). Areas of segmented clusters were compared to Clinician’s Erythema Assessment (CEA) values given by two dermatologists. The results show that visible blood vessels are segmented more precisely on maps of the erythema index and the third principal component (PC3). In many cases, a distribution of clusters on EI and PC3 maps are very similar. Mean values of clusters’ areas on these maps show a decrease of the area of blood vessels and erythema and an increase of lighter skin area after the therapy for the patients with diagnosis CEA = 2 on the first visit and CEA=1 on the second visit. This study shows that EI and PC3 maps are more useful than the maps of the first (PC1) and second (PC2) principal components for indicating vascular structures and erythema on the skin of rosacea patients and therapy monitoring.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124000940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monitoring soft tissue coagulation by optical spectroscopy 用光谱学监测软组织凝固
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297091
A. Lihachev, I. Lihacova, H. Heinrichs, J. Spigulis, T. Trebst, M. Wehner
{"title":"Monitoring soft tissue coagulation by optical spectroscopy","authors":"A. Lihachev, I. Lihacova, H. Heinrichs, J. Spigulis, T. Trebst, M. Wehner","doi":"10.1117/12.2297091","DOIUrl":"https://doi.org/10.1117/12.2297091","url":null,"abstract":"Laser tissue welding (LTW) or laser tissue soldering (LTS) is investigated since many years for treatment of incisions, wound closure and anastomosis of vessels [1, 2]. Depending on the process, a certain temperature in the range between 65 °C to 85 °C must be reached and held for a few seconds. Care has to be taken not to overheat the tissue, otherwise necrosis or tissue carbonization may occur and will impair wound healing. Usually the temperature is monitored during the process to control the laser power [3]. This requires either bulky equipment or expensive and fragile infrared fibers to feed the temperature signal to an infrared detector. Alternatively, changes in tissue morphology can be directly observed by analysis of spectral reflectance. We investigate spectral changes in the range between 400 nm to 900 nm wavelength. Characteristic spectral changes occur when the temperature of tissue samples increase above 70 °C which is a typical setpoint value for temperature control of coagulation. We conclude that simple spectroscopy in the visible range can provide valuable information during LTS and LTW and probably replace the delicate measurement of temperature. A major advantage is that optical measurements can be performed using standard optical fibers and can be easily integrated into a surgical tool.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"10592 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131065872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
pEGFP transfection into murine skeletal muscle by electrosonoporation 通过电晕法将 pEGFP 转染到小鼠骨骼肌中
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297222
M. Tamošiūnas, D. Jakovels, U. Rubins, R. Kadikis, R. Petrovska, S. Šatkauskas
{"title":"pEGFP transfection into murine skeletal muscle by electrosonoporation","authors":"M. Tamošiūnas, D. Jakovels, U. Rubins, R. Kadikis, R. Petrovska, S. Šatkauskas","doi":"10.1117/12.2297222","DOIUrl":"https://doi.org/10.1117/12.2297222","url":null,"abstract":"In this study, we aimed to determine whether the combination of electroporation (EP) and ultrasound (US) waves (sonoporation) can affect the plasmid DNA transfection to mice tibialis cranialis muscle. Multispectral imaging technique combined with fluorescence spectroscopy point measurements has been used for the transcutaneous detection of enhanced green fluorescent protein (EGFP) fluorescence, providing information on location and duration of EGFP expression. We found that electrosonoporation, commonly enhancing pDNA transfection in vitro, had no positive effect on EGFP transfection efficiency increase in vivo with respect to electroporation alone. We presume that this may be associated with decreased viability of transfected fibers.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"202 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131420147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Remote photoplethysmography system for unsupervised monitoring regional anesthesia effectiveness 用于无监督监测区域麻醉效果的远程光电容积脉搏波描记系统
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297158
U. Rubins, A. Miscuks, Z. Marcinkevics, M. Lange
{"title":"Remote photoplethysmography system for unsupervised monitoring regional anesthesia effectiveness","authors":"U. Rubins, A. Miscuks, Z. Marcinkevics, M. Lange","doi":"10.1117/12.2297158","DOIUrl":"https://doi.org/10.1117/12.2297158","url":null,"abstract":"Determining the level of regional anesthesia (RA) is vitally important to both an anesthesiologist and surgeon, also knowing the RA level can protect the patient and reduce the time of surgery. Normally to detect the level of RA, usually a simple subjective (sensitivity test) and complicated quantitative methods (thermography, neuromyography, etc.) are used, but there is not yet a standardized method for objective RA detection and evaluation. In this study, the advanced remote photoplethysmography imaging (rPPG) system for unsupervised monitoring of human palm RA is demonstrated. The rPPG system comprises compact video camera with green optical filter, surgical lamp as a light source and a computer with custom-developed software. The algorithm implemented in Matlab software recognizes the palm and two dermatomes (Medial and Ulnar innervation), calculates the perfusion map and perfusion changes in real-time to detect effect of RA. Seven patients (aged 18-80 years) undergoing hand surgery received peripheral nerve brachial plexus blocks during the measurements. Clinical experiments showed that our rPPG system is able to perform unsupervised monitoring of RA.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134161175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The silver layers in fiber-optic sensors 光纤传感器中的银层
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2295793
P. Listewnik, Melike Aydoğan, D. Majchrowicz, M. Jędrzejewska-Szczerska
{"title":"The silver layers in fiber-optic sensors","authors":"P. Listewnik, Melike Aydoğan, D. Majchrowicz, M. Jędrzejewska-Szczerska","doi":"10.1117/12.2295793","DOIUrl":"https://doi.org/10.1117/12.2295793","url":null,"abstract":"In this paper a method of deposition of the silver layers on the surface of an optical fiber was proposed. The optical properties and surface quality of the silver layer was examined by optical microscopy. The reflection and transmission of the sample were investigated. To investigate the quality the silver mirror it was placed in a fiber-optic Fabry-Perot interferometer and the quality of the spectra was analyzed. The commercial mirror was used as a reference reflective layer. Our studies confirm that the silver layer obtained in laboratory can be used for the application in the fiber-optic sensor and it is just as good as commercial mirror.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123962259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conjugation of fiber-coupled wide-band light sources and acousto-optical spectral elements 光纤耦合宽频带光源与声光光谱元件的共轭
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297608
A. Machikhin, V. Batshev, O. Polschikova, D. Khokhlov, V. Pozhar, A. Gorevoy
{"title":"Conjugation of fiber-coupled wide-band light sources and acousto-optical spectral elements","authors":"A. Machikhin, V. Batshev, O. Polschikova, D. Khokhlov, V. Pozhar, A. Gorevoy","doi":"10.1117/12.2297608","DOIUrl":"https://doi.org/10.1117/12.2297608","url":null,"abstract":"Endoscopic instrumentation is widely used for diagnostics and surgery. The imaging systems, which provide the hyperspectral information of the tissues accessible by endoscopes, are particularly interesting and promising for in vivo photoluminescence diagnostics and therapy of tumour and inflammatory diseases. To add the spectral imaging feature to standard video endoscopes, we propose to implement acousto-optical (AO) filtration of wide-band illumination of incandescent-lamp-based light sources. To collect maximum light and direct it to the fiber-optic light guide inside the endoscopic probe, we have developed and tested the optical system for coupling the light source, the acousto-optical tunable filter (AOTF) and the light guide. The system is compact and compatible with the standard endoscopic components.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"186 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115725091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Semi-automated non-invasive diagnostics method for melanoma differentiation from nevi and pigmented basal cell carcinomas 黑色素瘤与痣型和色素型基底细胞癌鉴别的半自动化无创诊断方法
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2295773
I. Lihacova, K. Boločko, A. Lihachev
{"title":"Semi-automated non-invasive diagnostics method for melanoma differentiation from nevi and pigmented basal cell carcinomas","authors":"I. Lihacova, K. Boločko, A. Lihachev","doi":"10.1117/12.2295773","DOIUrl":"https://doi.org/10.1117/12.2295773","url":null,"abstract":"The incidence of skin cancer is still increasing mostly in in industrialized countries with light- skinned people. Late tumour detection is the main reason of the high mortality associated with skin cancer. The accessibility of early diagnostics of skin cancer in Latvia is limited by several factors, such as high cost of dermatology services, long queues on state funded oncologist examinations, as well as inaccessibility of oncologists in the countryside regions - this is an actual clinical problem. The new strategies and guidelines for skin cancer early detection and post-surgical follow-up intend to realize the full body examination (FBE) by primary care physicians (general practitioners, interns) in combination with classical dermoscopy. To implement this approach, a semi- automated method was established. Developed software analyses the combination of 3 optical density images at 540 nm, 650 nm, and 950 nm from pigmented skin malformations and classifies them into three groups- nevi, pigmented basal cell carcinoma or melanoma.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"4 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116762216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Microscopic and macroscopic spectral peculiarities of cutaneous tumours 皮肤肿瘤的显微和宏观光谱特征
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2296562
E. Borisova, Tsanislava Genova, P. Troyanova, I. Terziev, V. Zakharov, I. Bratchenko, M. Lomova, D. Gorin, L. Avramov
{"title":"Microscopic and macroscopic spectral peculiarities of cutaneous tumours","authors":"E. Borisova, Tsanislava Genova, P. Troyanova, I. Terziev, V. Zakharov, I. Bratchenko, M. Lomova, D. Gorin, L. Avramov","doi":"10.1117/12.2296562","DOIUrl":"https://doi.org/10.1117/12.2296562","url":null,"abstract":"Autofluorescence spectral and confocal microscopic measurements were made on different cutaneous neoplastic lesions, namely basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and their dysplastic forms – keratoacantoma, dysplastic nevi and benign lesions related – basal cell papiloma, seboreic keratosa and compound nevi using excitation at 405 nm. Spectroscopic investigations were made on in vivo and ex vivo tissue samples, and confocal microscopy investigations were made on ex vivo and eosin-haematoxilin stained thin slices of the tumours detected. Correlation between the spectral data received and the microscopic features observed was found, related to the morphological and biochemical alterations during neoplasia growth. Specific spectral features observed in each type of lesion investigated on micro and macro level would be presented and discussed.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128042983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of skin pathologies by RGB autofluorescence imaging RGB自体荧光成像对皮肤病变的评价
Biophotonics-Riga Pub Date : 2017-12-07 DOI: 10.1117/12.2297007
A. Lihachev, E. V. Plorina, A. Derjabo, M. Lange, I. Lihacova
{"title":"Evaluation of skin pathologies by RGB autofluorescence imaging","authors":"A. Lihachev, E. V. Plorina, A. Derjabo, M. Lange, I. Lihacova","doi":"10.1117/12.2297007","DOIUrl":"https://doi.org/10.1117/12.2297007","url":null,"abstract":"A clinical trial on autofluorescence imaging of malignant and non-malignant skin pathologies comprising 32 basal cell carcinomas (BCC), 4 malignant melanomas (MM), 1 squamous cell carcinoma (SCC), 89 nevi, 14 dysplastic nevi, 20 hemangiomas, 23 seborrheic keratoses, 4 hyperkeratoses, 3 actinic keratoses, 3 psoriasis, 1 dematitis, 2 dermatofibromas, 5 papillofibromas, 12 lupus erythematosus, 7 purpura, 6 bruises, 5 freckles, 3 fungal infections, 1 burn, 1 tattoo, 1 age spot, 1 vitiligo, 32 postoperative scars, 8 post cream therapy BCCs, 4 post radiation therapy scars, 2 post laser therapy scars, 1 post freezing scar as well as 114 reference images of healthy skin was performed. The sequence of autofluorescence images of skin pathologies were recorded by smartphone RGB camera under continuous 405 nm LED excitation during 20 seconds with 0.5 fps. Obtained image sequences further were processed with subsequent extraction of autofluorescence intensity and photobleaching parameters.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130508452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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