通过电晕法将 pEGFP 转染到小鼠骨骼肌中

M. Tamošiūnas, D. Jakovels, U. Rubins, R. Kadikis, R. Petrovska, S. Šatkauskas
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引用次数: 0

摘要

在这项研究中,我们旨在确定电穿孔(EP)和超声波(US)的结合(sonoporation)是否会影响小鼠胫腓骨肌的质粒 DNA 转染。多光谱成像技术与荧光光谱点测量相结合,用于经皮检测增强型绿色荧光蛋白(EGFP)荧光,从而提供有关 EGFP 表达位置和持续时间的信息。我们发现,在体外通常能增强 pDNA 转染的电穿孔对提高体内 EGFP 转染效率没有积极影响。我们推测这可能与转染纤维的活力下降有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
pEGFP transfection into murine skeletal muscle by electrosonoporation
In this study, we aimed to determine whether the combination of electroporation (EP) and ultrasound (US) waves (sonoporation) can affect the plasmid DNA transfection to mice tibialis cranialis muscle. Multispectral imaging technique combined with fluorescence spectroscopy point measurements has been used for the transcutaneous detection of enhanced green fluorescent protein (EGFP) fluorescence, providing information on location and duration of EGFP expression. We found that electrosonoporation, commonly enhancing pDNA transfection in vitro, had no positive effect on EGFP transfection efficiency increase in vivo with respect to electroporation alone. We presume that this may be associated with decreased viability of transfected fibers.
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