Microscopic and macroscopic spectral peculiarities of cutaneous tumours

Abstract

Autofluorescence spectral and confocal microscopic measurements were made on different cutaneous neoplastic lesions, namely basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and their dysplastic forms – keratoacantoma, dysplastic nevi and benign lesions related – basal cell papiloma, seboreic keratosa and compound nevi using excitation at 405 nm. Spectroscopic investigations were made on in vivo and ex vivo tissue samples, and confocal microscopy investigations were made on ex vivo and eosin-haematoxilin stained thin slices of the tumours detected. Correlation between the spectral data received and the microscopic features observed was found, related to the morphological and biochemical alterations during neoplasia growth. Specific spectral features observed in each type of lesion investigated on micro and macro level would be presented and discussed.