Biophotonics-Riga最新文献_第8页

Novel combined fluorescence/reflectance spectroscopy system for guiding brain tumor resections: hardware considerations 用于指导脑肿瘤切除的新型联合荧光/反射光谱系统:硬件考虑

Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044717

Zhiyuan Xie, Haiyan Xie, Monirehalsadat Mousavi, M. Brydegaard, J. Axelsson, S. Andersson-Engels

引用次数: 0

Water detection in skin by dual-band photodiodes 双波段光电二极管检测皮肤水分

Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2045361

I. Saknite, E. Kviesis, J. Spigulis

引用次数: 0

Excitation-emission matrices measurements of human cutaneous lesions: tool for fluorescence origin 人体皮肤病变的激发-发射矩阵测量:荧光起源工具

Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044450

A. Zhelyazkova, E. Borisova, L. Angelova, E. Pavlova, M. Keremedchiev

{"title":"Excitation-emission matrices measurements of human cutaneous lesions: tool for fluorescence origin","authors":"A. Zhelyazkova, E. Borisova, L. Angelova, E. Pavlova, M. Keremedchiev","doi":"10.1117/12.2044450","DOIUrl":"https://doi.org/10.1117/12.2044450","url":null,"abstract":"The light induced fluorescence (LIF) technique has the potential of providing real-time diagnosis of malignant and premalignant skin tissue; however, human skin is a multilayered and inhomogeneous organ with different optical properties that complicate the analysis of cutaneous fluorescence spectra. In spite of the difficulties related to the detection and analysis of fluorescent data from skin lesions, this technique is among the most widely applied techniques in laboratorial and pre-clinical investigations for early skin neoplasia diagnosis. The important point is to evaluate all sources of intrinsic fluorescence and find any significant alterations distinguishing the normal skin from a cancerous state of the tissue; this would make the autofluorescence signal obtained useful for the development of a non-invasive diagnostic tool for the dermatological practice. Our investigations presented here were based on ex vivo point-by-point measurements of excitation-emission matrices (EEM) from excised tumor lesions and the surrounding skin taken during the daily clinical practice of Queen Jiovanna- ISUL University Hospital, Sofia, the local Ethical Committee’s approval having already been obtained. The fluorescence emission was measured between 300 nm and 800 nm using excitation in the 280–440 nm spectral range. In the process of excitation-emission matrices (EEM) measurements we could establish the origin of the autofluorescence and the compounds related by assigning the excitation and emission maxima obtained during the experiments. The EEM were compared for normal human skin, basal cell carcinoma, squamous cell carcinoma, benign nevi and malignant melanoma lesions to obtain information for the most common skin malignancies and their precursors. The main spectral features and the applicability of the technique of autofluorescent spectroscopy of human skin in general as an initial diagnostic tool are discussed as well.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121739627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Modelling the appearance of chromatic environment using hyperspectral imaging 利用高光谱成像技术对彩色环境的外观进行建模

Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044679

S. Fomins, M. Ozolinsh

{"title":"Modelling the appearance of chromatic environment using hyperspectral imaging","authors":"S. Fomins, M. Ozolinsh","doi":"10.1117/12.2044679","DOIUrl":"https://doi.org/10.1117/12.2044679","url":null,"abstract":"Color of objects is a spectral composition of incident light source, reflection properties of the object itself, and spectral tuning of the eye. Light sources with different spectral characteristics can produce metameric representation of color; however most variable in this regard is vision. Pigments of color vision are continuously bleached by different stimuli and optical density of the pigment is changed, while continuous conditions provide an adaptation and perception of white. Special cases are color vision deficiencies which cover almost 8 % of male population in Europe. Hyperspectral imaging allows obtaining the spectra of the environment and modelling the performance of the dichromatic, anomalous trichromatic, as also normal trichromatic adapted behavior. First, CRI Nuance hyperspectral imaging system was spectrally calibrated for natural continuous spectral illumination of high color rendering index and narrow band fluorescent light sources. Full-scale images of color deficiency tests were acquired in the range of 420 to 720 nm to evaluate the modelling capacity for dichromatic and anomalous trichromatic vision. Hyperspectral images were turned to cone excitation images according to Stockman and Sharpe (2000) 1. Further, model was extended for anomalous trichromacy conditions. Cone sensitivity spectra were shifted by 4 nm according to each anomaly type. LWS and SWS cone signals were balanced in each condition to provide the appropriate appearance of colors in CIE system.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130033549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Elastic light single-scattering spectroscopy for detection of dysplastic tissues 弹性光单散射光谱法检测发育不良组织

Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044681

M. Canpolat, T. Denkçeken, A. Akman, E. Alpsoy, R. Tuncer, M. Akyüz, M. Baykara, Selçuk Yücel, I. Başsorgun, M. Çiftçioğlu, G. Gökhan, E. Gürer, E. Pestereli, S. Karaveli

{"title":"Elastic light single-scattering spectroscopy for detection of dysplastic tissues","authors":"M. Canpolat, T. Denkçeken, A. Akman, E. Alpsoy, R. Tuncer, M. Akyüz, M. Baykara, Selçuk Yücel, I. Başsorgun, M. Çiftçioğlu, G. Gökhan, E. Gürer, E. Pestereli, S. Karaveli","doi":"10.1117/12.2044681","DOIUrl":"https://doi.org/10.1117/12.2044681","url":null,"abstract":"Elastic light single-scattering spectroscopy (ELSSS) system has been developed and tested in diagnosis of cancerous tissues of different organs. ELSSS system consists of a miniature visible light spectrometer, a single fiber optical probe, a halogen tungsten light source and a laptop. Measurements were performed on excised brain, skin, cervix and prostate tumor specimens and surrounding normal tissues. Single fiber optical probe with a core diameter of 100 μm was used to deliver white light to and from tissue. Single optical fiber probe mostly detects singly scattered light from tissue rather than diffused light. Therefore, measured spectra are sensitive to size of scatters in tissue such as cells, nuclei, mitochondria and other organelles of cells. Usually, nuclei of tumor cells are larger than nuclei of normal cells. Therefore, spectrum of singly scattered light of tumor tissue is different than normal tissue. The spectral slopes were shown to be positive for normal brain, skin and prostate and cervix tissues and negative for the tumors of the same tissues. Signs of the spectral slopes were used as a discrimination parameter to differentiate tumor from normal tissues for the three organ tissues. Sensitivity and specificity of the system in differentiation between tumors from normal tissues were 93% and %100 for brain, 87% and 85% for skin, 93.7% and 46.1% for cervix and 98% and 100% for prostate.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124221415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1