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Assessment of hemoglobin dynamics in traumatic bruises using temperature depth profiling 利用温度深度分析评估创伤性瘀伤中的血红蛋白动态
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044682
L. Vidovič, M. Milanič, B. Majaron
{"title":"Assessment of hemoglobin dynamics in traumatic bruises using temperature depth profiling","authors":"L. Vidovič, M. Milanič, B. Majaron","doi":"10.1117/12.2044682","DOIUrl":"https://doi.org/10.1117/12.2044682","url":null,"abstract":"Perceived color of traumatic bruise depends strongly on depth of the spilled blood, natural skin tone, ambient light conditions, etc., which prevents an accurate and reliable determination of the time of the injury. Pulsed photothermal radiometry (PPTR) allows noninvasive determination of the laser-induced temperature depth profile in human skin. We have applied this technique to characterize dynamics of extravasated hemoglobin in the bruise. Next, we use simple model of mass diffusion and biochemical transformation kinetics to simulate bruise dynamics. By applying Monte Carlo simulation of laser energy deposition, comparison with measured temperature profiles is possible. However, parameters of the model were previously not determined directly. Instead, biologically plausible values were assumed. We show how temperature depth profiling enables accurate monitoring of hemoglobin diffusion and degradation. Parameters of the model, hemoglobin mass diffusivity, hemoglobin degradation time, and skin geometry, can be estimated rather accurately. Derivation of bruise evolution parameters will be a valuable addition to existing bruise age determination techniques.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"284 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122966958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Express control of plants general state by using the new generation of the instrumental tools 利用新一代仪器工具实现对植物总体状态的表达控制
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044686
K. E. Shavanova, M. V. Taran, O. Marchenko, N. F. Starodub
{"title":"Express control of plants general state by using the new generation of the instrumental tools","authors":"K. E. Shavanova, M. V. Taran, O. Marchenko, N. F. Starodub","doi":"10.1117/12.2044686","DOIUrl":"https://doi.org/10.1117/12.2044686","url":null,"abstract":"The purpose of the present study was to determine the effectiveness of the chlorophyll fluorescence method induction for the estimation of the functional state of the photosynthetic activity of plants. The study was done using the \"Floratest\" prototype portable device. It was found that the representative plants in three ecological zones have a significant difference of indexes, what, through the changes of the chlorophyll fluorescence induction, reflects the processes of energy transformation at the early stages of photosynthesis. It was stated that value of Kpl plateau in the Kautsky curve can be used as a test for the early selection of the horse chestnut forms, resistant to the influence of the complex environmental factors. The results in bean leaves indicated that the fluorescent indices are sensitive to the effects of such stressors as acidification, salinity, dehydration.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125600916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Multimodal device for assessment of skin malformations 评估皮肤畸形的多模态装置
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044142
A. Bekina, V. Garancis, U. Rubins, J. Spigulis, L. Valeine, A. Berzina
{"title":"Multimodal device for assessment of skin malformations","authors":"A. Bekina, V. Garancis, U. Rubins, J. Spigulis, L. Valeine, A. Berzina","doi":"10.1117/12.2044142","DOIUrl":"https://doi.org/10.1117/12.2044142","url":null,"abstract":"A variety of multi-spectral imaging devices is commercially available and used for skin diagnostics and monitoring; however, an alternative cost-efficient device can provide an advanced spectral analysis of skin. A compact multimodal device for diagnosis of pigmented skin lesions was developed and tested. A polarized LED light source illuminates the skin surface at four different wavelengths – blue (450 nm), green (545 nm), red (660 nm) and infrared (940 nm). Spectra of reflected light from the 25 mm wide skin spot are imaged by a CMOS sensor. Four spectral images are obtained for mapping of the main skin chromophores. The specific chromophore distribution differences between different skin malformations were analyzed and information of subcutaneous structures was consecutively extracted.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121967475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TiO2 optical sensor for amino acid detection 用于氨基酸检测的TiO2光学传感器
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044464
A. Tereshchenko, R. Viter, I. Konup, V. Ivanitsa, S. Geveliuk, Y. Ishkov, V. Smyntyna
{"title":"TiO2 optical sensor for amino acid detection","authors":"A. Tereshchenko, R. Viter, I. Konup, V. Ivanitsa, S. Geveliuk, Y. Ishkov, V. Smyntyna","doi":"10.1117/12.2044464","DOIUrl":"https://doi.org/10.1117/12.2044464","url":null,"abstract":"A novel optical sensor based on TiO2 nanoparticles for Valine detection has been developed. In the presented work, commercial TiO2 nanoparticles (Sigma Aldrich, particle size 32 nm) were used as sensor templates. The sensitive layer was formed by a porphyrin coating on a TiO2 nanostructured surface. As a result, an amorphous layer between the TiO2 nanostructure and porphyrin was formed. Photoluminescence (PL) spectra were measured in the range of 370-900 nm before and after porphyrin application. Porphyrin adsorption led to a decrease of the main TiO2 peak at 510 nm and the emergence of an additional peak of high intensity at 700 nm. Absorption spectra (optical density vs. wavelenght, measured from 300 to 1100 nm) showed IR shift Sorret band of prophiryn after deposition on metal oxide. Adsorption of amino acid quenched PL emission, related to porphyrin and increased the intensity of the TiO2 emission. The interaction between the sensor surface and the amino acid leads to the formation of new complexes on the surface and results in a reduction of the optical activity of porphyrin. Sensitivity of the sensor to different concentrations of Valine was calculated. The developed sensor can determine the concentration of Valine in the range of 0.04 to 0.16 mg/ml.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115402572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Evaluation of a multispectral diffuse optical spectroscopy device for assessment of cardiometabolic risk related alterations of body composition 评估一种多光谱漫射光谱学装置，用于评估与心脏代谢风险相关的身体成分改变
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044685
Z. Marcinkevics, K. Volceka, L. Ozoliņa-Moll, J. Zaharans
{"title":"Evaluation of a multispectral diffuse optical spectroscopy device for assessment of cardiometabolic risk related alterations of body composition","authors":"Z. Marcinkevics, K. Volceka, L. Ozoliņa-Moll, J. Zaharans","doi":"10.1117/12.2044685","DOIUrl":"https://doi.org/10.1117/12.2044685","url":null,"abstract":"Cardiometabolic diseases encompass a combination of conditions which lead to an increase in the risk of cardiovascular disease and diabetes. With the increasing percentage of the population becoming overweight, it is important to diagnose when the excess adipose tissue becomes malign. The development of a safe, mobile, non-invasive method that would be easy to perform, and low-cost, but also would offer an accurate assessment of subcutaneous adipose tissue (SAT) both in lean and in obese persons is required. A prototype device using an optical method for measurement of the SAT in vivo has been developed, it contains multiple LEDs with four wavelengths (660nm, 780nm, 870nm, 940nm) distributed at various distances from the photodetector which allow different light penetration depths into the subcutaneous tissue. Five young healthy female students participated in the study; the measurements were performed on three body sites: calf, upper and lower abdomen. The backscattered light acquired with the prototype was compared to SAT measured with high resolution ultrasound imaging. The coefficient of variation indicated high reliability of the measurements. Statistically significant (from r=0.81 to r=0.95; p<0.05) correlation between intensity of backscattered light and SAT thicknesses for all four wavelength was observed, especially at source-detector distance 25mm. The novel device prototype has a potential to be a good alternative for conventional SAT measurement and assessment of cardiometabolic risk. Amultispectral approach can potentially increase precision and spatial resolution of SAT determination.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116469404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reliability and validity of optoelectronic method for biophotonical measurements 光电测量生物光子学方法的可靠性和有效性
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044608
K. Karpienko, M. Wróbel, R. Urniaz
{"title":"Reliability and validity of optoelectronic method for biophotonical measurements","authors":"K. Karpienko, M. Wróbel, R. Urniaz","doi":"10.1117/12.2044608","DOIUrl":"https://doi.org/10.1117/12.2044608","url":null,"abstract":"Reliability and validity of measurements is of utmost importance when assessing measuring capability of instruments developed for research. In order to perform an experiment which is legitimate, used instruments must be both reliable and valid. Reliability estimates the degree of precision of measurement, the extent to which a measurement is internally consistent. Validity is the usefulness of an instrument to perform accurate measurements of quantities it was designed to measure. Statistical analysis for reliability and validity control of low-coherence interferometry method for refractive index measurements of biological fluids is presented. The low-coherence interferometer is sensitive to optical path difference between interfering beams. This difference depends on the refractive index of measured material. To assess the validity and reliability of proposed method for blood measurements, the statistical analysis of the method was performed on several substances with known refractive indices. Analysis of low-coherence interferograms considered the mean distances between fringes. Performed statistical analysis for validity and reliability consisted of Grubb’s test for outliers, Shapiro-Wilk test for normal distribution, T-Student test, standard deviation, coefficient of determination and r-Pearson correlation. Overall the tests proved high statistical significance of measurement method with confidence level < 0.0001 of measurement method.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129668783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Methodology for assessment of low level laser therapy (LLLT) irradiation parameters in muscle inflammation treatment 评估低水平激光治疗(LLLT)在肌肉炎症治疗中的辐照参数方法学
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044689
M. Mantineo, J. Pinheiro, A. M. Morgado
{"title":"Methodology for assessment of low level laser therapy (LLLT) irradiation parameters in muscle inflammation treatment","authors":"M. Mantineo, J. Pinheiro, A. M. Morgado","doi":"10.1117/12.2044689","DOIUrl":"https://doi.org/10.1117/12.2044689","url":null,"abstract":"Several studies in human and animals show the clinical effectiveness of low level laser therapy (LLLT) in reducing some types of pain, treating inflammation and wound healing. However, more scientific evidence is required to prove the effectiveness of LLLT since many aspects of the cellular and molecular mechanisms triggered by irradiation of injured tissue with laser remain unknown. Here, we present a methodology that can be used to evaluate the effect of different LLLT irradiation parameters on the treatment of muscle inflammation on animals, through the quantification of four cytokines (TNF-α, IL-1β, IL-2 and IL-6) in systemic blood and histological analysis of muscle tissue. We have used this methodology to assess the effect of LLLT parameters (wavelength, dose, power and type of illumination) in the treatment of inflammation induced in the gastrocnemius muscle of Wistar rats. Results obtained for laser dose evaluation with continuous illumination are presented.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131703787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of efficiencies of electroporation and sonoporation methods by using fluorescence RGB imaging method 用荧光RGB成像方法评价电穿孔和超声穿孔方法的效率
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044385
D. Jakovels, A. Lihachev, J. Spigulis, S. Šatkauskas, M. Tamošiūnas, C. Lo, W. S. Chen
{"title":"Assessment of efficiencies of electroporation and sonoporation methods by using fluorescence RGB imaging method","authors":"D. Jakovels, A. Lihachev, J. Spigulis, S. Šatkauskas, M. Tamošiūnas, C. Lo, W. S. Chen","doi":"10.1117/12.2044385","DOIUrl":"https://doi.org/10.1117/12.2044385","url":null,"abstract":"Simple RGB method for fluorescence in vivo imaging is presented to assess efficiency of electroporation and sonoporation methods by measuring distribution and accumulation of green fluorescence protein (GFP) concentration. 20 laboratory measurements were performed on mice to test the method.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"135 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116118934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Spatial photometry of scattered radiation by biological objects 生物物体散射辐射的空间光度测定
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044609
N. Bezuglaya, M. Bezuglyi
{"title":"Spatial photometry of scattered radiation by biological objects","authors":"N. Bezuglaya, M. Bezuglyi","doi":"10.1117/12.2044609","DOIUrl":"https://doi.org/10.1117/12.2044609","url":null,"abstract":"The work is devoted to the analysis of tools for the research of the indicatrix of scattering optical radiation by biological objects and their systematization. Simulation of system of spatial photometry for biomedical diagnostics which is designed to research the scattering within the solid angle 4π and the system of determining the optimal photometric distance of various biological structures were made.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121801476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Dedicated power supply subsystem for ultra-low noise preamplifiers and biophotonic sensors 超低噪声前置放大器和生物光子传感器专用电源子系统
Biophotonics-Riga Pub Date : 2013-11-18 DOI: 10.1117/12.2044592
Łukasz Surażyński, P. Wierzba, A. Zienkiewicz
{"title":"Dedicated power supply subsystem for ultra-low noise preamplifiers and biophotonic sensors","authors":"Łukasz Surażyński, P. Wierzba, A. Zienkiewicz","doi":"10.1117/12.2044592","DOIUrl":"https://doi.org/10.1117/12.2044592","url":null,"abstract":"It is very common for noise to have an influence on analog circuits. In order to preserve the quality of measurements taken by specific sensors and any noise dependent amplifiers which are correlated to them, all of these devices must be powered by low-noise power supplies. Therefore a necessity exists to develop new ultra-low noise power supplies which can cooperate with specified amplifiers and preamplifiers. Many well-known power supplies are particularly expensive and yet still have their disadvantages. This paper proposes a simple and inexpensive solution, which fulfills a specific criteria and can be treated as a base for improvement.","PeriodicalId":283327,"journal":{"name":"Biophotonics-Riga","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122443443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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