Chinese Journal of Analytical Chemistry最新文献

{"title":"Integrated network pharmacology, molecular docking, and experiments in vivo and in vitro to explore the efficacy and potential mechanism of Chrysanthemum indicum L. extract against glioma","authors":"Man ZHAO , Dandan HU , Rubing LI , Chen GONG , Xuegang ZHOU , Xiaoying LIU , Shukun TANG , Chenxi ZHOU , Yue HAO , Jialin FANG , Xu LIU , Haotian CHEN , Haisheng PENG , Na WANG , Wenyuan ZHANG","doi":"10.1016/j.cjac.2025.100666","DOIUrl":"10.1016/j.cjac.2025.100666","url":null,"abstract":"<div><h3>Background</h3><div><em>Chrysanthemum indicum</em> L., a herbaceous perennial plant in the Asteraceae family, has been found to possess anti-inflammatory and anticancer effects. Jiawei Juming Decoction (JWJM), composed of <em>Chrysanthemum indicum</em> L., <em>Senna tora</em> (L.) <em>Roxb., Forsythia suspensa</em> (Thunb.) Vahl, <em>Astragalus membranaceus</em> (Fisch.) Bunge, etc., has been utilized in the clinical for treating glioma in China. However, as an important medicinal material, the efficacy of <em>Chrysanthemum indicum</em> L. against glioma have not been reported. In this study, a multi-faceted research approach encompassing <em>in vitro</em> and <em>in vivo</em> experimentation, network pharmacology, and molecular docking was employed to elucidate the anti-glioma effects of <em>Chrysanthemum indicum</em> L. extract (CIE) and the changes in related target proteins within Pathways in cancer.</div></div><div><h3>Methods</h3><div>Active ingredients in CIE were identified using the TCMSP and ETCM databases. The GeneCards and DisGeNET databases were utilized to identify targets associated with glioma, followed by creating a Venn map to pinpoint common drug-disease targets. The STRING database was employed for analyzing protein-protein interaction (PPI) and screening key targets. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed for the enrichment analyses. Cytoscape software was utilized to create a compound-target-pathway network aimed at discovering important active components and potential mechanisms. The binding strength between the key target and CIE’s active compounds was verified via molecular docking simulation. The predicted results were confirmed by cell experiments, and the influence of CIE on glioma cell proliferation, migration, apoptosis, as well as antitumor activity were further evaluated through <em>in vivo</em> and <em>in vitro</em> experiments.</div></div><div><h3>Results</h3><div>The CIE contains 23 active compounds and 130 major targets, identifying nine key targets: ESR1, SIRT1, HSP90AA1, PTGS2, RELA, AR, NOS3, DNMT1, and GSK3B. The effectiveness of CIE treatment is primarily focused on the Pathways in cancer, as highlighted by GO and KEGG enrichment analysis. It was confirmed through protein expression that Androgen receptor (AR) is essential for glioma progression. Molecular docking showed that kaempferol, naringenin and luteolin strongly bound to AR. The influence of CIE on the target protein was confirmed through <em>in vitro</em> experiments. It has been demonstrated that CIE has the capacity to inhibit the proliferation and migration of C6 cells, whilst concomitantly inducing an increased rate of cell apoptosis. Experiments with animals revealed that CIE could initiate apoptosis in glioma cells and possessed anti-tumor properties.</div></div><div><h3>Conclusion</h3><div>By influencing the relevant target protein (AR), the proliferation of C6 cells could be reduce","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 2","pages":"Article 100666"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential molecular mechanism of qingying decoction against monkeypox via network pharmacology and molecular docking","authors":"Zhuo WANG ,&nbsp;Jiandong CHEN ,&nbsp;Chunhui DENG","doi":"10.1016/j.cjac.2025.100635","DOIUrl":"10.1016/j.cjac.2025.100635","url":null,"abstract":"<div><h3>Background</h3><div>The global spread of monkeypox virus (MPXV) has exposed the scarcity of targeted therapeutic agents, highlighting an urgent medical need. Qingying Decoction (QYD) is one of the traditional Chinese medicine (TCM) formulas recommended for the treatment of monkeypox in the Guidelines for the Treatment of Monkeypox (2022 Edition) issued by the National Health Commission (NHC) of the People’s Republic of China. However, its potential molecular mechanism against MPXV remains unclear, and in-depth exploration is urgently needed to tap its therapeutic potential.</div></div><div><h3>Methods</h3><div>This study integrated network pharmacology and molecular docking techniques: bioactive components (BICs) of QYD were screened and their targets predicted; MPXV-related targets were obtained from databases and datasets; common targets of QYD and MPXV were identified, followed by GO/KEGG enrichment analysis; a protein-protein interaction (PPI) network was constructed to screen hub targets, and \"TCM-BIC-target-pathway\" related networks were built; finally, molecular docking was used to verify the binding ability between core components and targets.</div></div><div><h3>Results</h3><div>A total of 591 BICs of QYD were screened out, and 78 common targets between QYD and MPXV were identified. These common targets were significantly enriched in immune and inflammatory-related pathways such as TLR, TNF, and NF-<em>κ</em>B; meanwhile, 15 key BICs and 8 key targets were screened. Molecular docking results showed that core BICs including cis-ferulic acid, ferulic acid methyl ester, and kaempferol had strong binding ability to key targets such as NFKB1 and PTGS2.</div></div><div><h3>Conclusion</h3><div>QYD exerts synergistic anti-MPXV effects through a \"multi-component, multi-target, multi-pathway\" mode. Its core components act on key targets to regulate core pathways, with effects of reversing viral immune evasion, blocking viral invasion, and alleviating inflammatory storms, and it outperforms some other traditional Chinese medicine formulas. This study provides a theoretical basis for the development of TCM-based anti-MPXV therapies, but further <em>in vitro</em> and <em>in vivo</em> experiments are needed to verify the efficacy and mechanism of its core components.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 2","pages":"Article 100635"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of combined paeoniflorin and glycyrrhizin therapy for ulcerative colitis in vitro and in vivo","authors":"Yuheng TSENG ,&nbsp;Qiuping XIAO ,&nbsp;Feng WU ,&nbsp;Meihui HUANG ,&nbsp;Xiaoyun WU ,&nbsp;Jinrong LIN ,&nbsp;Yanqiu HONG ,&nbsp;Lizhu LIN","doi":"10.1016/j.cjac.2025.100633","DOIUrl":"10.1016/j.cjac.2025.100633","url":null,"abstract":"<div><div>Ulcerative colitis (UC), a chronic inflammatory bowel disease, severely affects the quality of life of patients. Current treatments, although effective for symptom relief, are limited by side effects and efficacy constraints. Paeoniflorin (PA) and glycyrrhizin (GL), derived from traditional Chinese medicine, exhibit anti-inflammatory and antioxidant activities. Previous studies have indicated that their combination may offer synergistic effects in UC treatment. This study aimed to evaluate the efficacy of PA and GL in combination for UC therapy and to explore the underlying mechanisms. Network pharmacology analysis revealed that PA and GL modulated multiple UC-related targets, influencing inflammatory responses and cytokine activities. <em>In vitro</em> experiments demonstrated that the PA and GL combination enhanced cell viability and suppressed inflammatory factor production. In a dextran sulfate sodium (DSS)-induced UC mouse model, combination therapy significantly improved colon length, reduced the disease activity index (DAI), and modulated inflammation-associated cytokine levels. Compared to conventional UC treatments, PA and GL combination therapy more precisely targets inflammatory pathways. It effectively reduces pro-inflammatory cytokines (<em>IL1B, IL6</em>, and <em>TNF</em>) and increases anti-inflammatory cytokine (<em>IL10</em>) levels. This study enhances our understanding of traditional Chinese medicine combinations in UC treatment and offers a promising new clinical strategy that has the potential to improve UC patient outcomes and reduce adverse effects.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 2","pages":"Article 100633"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and application of Mn, Si-CQDs in vitamin B6 and in vitro activity analysis","authors":"Lifen MENG ,&nbsp;Yinying ZHANG ,&nbsp;Mengting SU","doi":"10.1016/j.cjac.2025.100634","DOIUrl":"10.1016/j.cjac.2025.100634","url":null,"abstract":"<div><div>The determination of vitamin B6 (VB6) in food, health products, and pharmaceuticals is of great significance because it plays a crucial role in maintaining health and is a necessary condition for dietary intake. Therefore, the study uses <span><em>l</em></span>-histidine, citric acid, potassium permanganate, and tetraethyl silicate as carbon source and Mn, Si source to prepare <em>Mn, Si-CQDs</em> by a hydrothermal method. The characteristics of the <em>Mn, Si-CQDs</em> are studied by Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and Ultraviolet-visible (UV–vis) spectrum, <em>Mn, Si-CQDs</em> are spherical and well dispersed in aqueous solution, with an average particle size of 5 nm. The effect of different pH and storage time on the fluorescence intensity of <em>Mn, Si-CQDs</em> indicates that <em>Mn, Si-CQDs</em> have good fluorescence stability. The fluorescence of <em>Mn, Si-CQDs</em> can be selectively quenched by VB6 without being affected by interfering other substances, within the range of 0.05–20 μg/mL, The quenching degree ratio of <em>Mn, Si-CQDs</em>, F₀/F, shows a good linear relationship with the concentration of VB6 (<em>F</em>₀/<em>F</em>=0.19311+0.03723<em>C</em> (<em>R</em>²=0.99882)), with a detection limit of 0.037 μg/mL. The recovery rates were 94.21%–98.80% by the spiked recovery experiments, indicating the high sensitivity and selectivity of the <em>Mn, Si-CQDs</em>. During the antibacterial test, the <em>Mn, Si-CQDs</em> exhibite good antibacterial effects on <em>Staphylococcus aureus, Penicillium, Escherichia coli</em> and <em>Saccharomyces cerevisiae</em>.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 2","pages":"Article 100634"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in ZnO/MOFs: Synthesis, characterization, and applications in sustainable antibiotic wastewater treatment","authors":"Roushree RR,&nbsp;Rijah HAIMBODI","doi":"10.1016/j.cjac.2025.100619","DOIUrl":"10.1016/j.cjac.2025.100619","url":null,"abstract":"<div><div>The increasing prevalence of antibiotic pollutants, such as amoxicillin, tetracycline, ciprofloxacin, and sulfamethoxazole, in wastewater poses significant environmental and health risks, including the promotion of antibiotic resistance. This review consolidates recent progress in the synthesis, characterization, and application of zinc oxide (ZnO)/metal-organic framework (MOF) composites as highly efficient photocatalysts and adsorbents for sustainable antibiotic wastewater treatment. ZnO/MOF hybrids achieve degradation efficiencies often exceeding 90 % under optimized conditions, driven by synergistic adsorption (via <em>π</em>-<em>π</em> interactions, electrostatic forces, and pore confinement) and photocatalysis (via reactive oxygen species like •OH and •O₂⁻). Synthesis methods, including in-situ growth, solvothermal, and microwave-assisted techniques, enable tailored composite structures, while advanced characterization (X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), UV-Vis/NIR) elucidates their structural and chemical synergies. Compared to TiO₂, g-C₃N₄, and graphene-based materials, ZnO/MOFs offer superior tunability and efficiency but face challenges in visible light absorption, aqueous stability, and scalability. These composites demonstrate significant potential for cost-effective, eco-friendly wastewater treatment, reducing antibiotic residues and mitigating resistance proliferation. Future research should focus on enhancing visible light activity, green synthesis, and real-world testing to ensure scalability and environmental safety, positioning ZnO/MOFs as a sustainable solution for global water purification challenges.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 1","pages":"Article 100619"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing inorganic halide perovskite quantum dots: Breakthroughs in green synthesis, stabilization, and sustainability perspectives","authors":"Mohammad ABUSHUHEL ,&nbsp;Ms.G. PADMAPRIYA ,&nbsp;Al-Hasnaawei SHAKER ,&nbsp;Subhashree RAY ,&nbsp;Kattela CHENNAKESAVULU ,&nbsp;Renu SHARMA ,&nbsp;Ashish Singh CHAUHAN ,&nbsp;Shaima MESSA","doi":"10.1016/j.cjac.2025.100679","DOIUrl":"10.1016/j.cjac.2025.100679","url":null,"abstract":"<div><div>Inorganic halide perovskite quantum dots (IHPQDs), such as CsPbX₃ (X = Cl, Br, I), are pivotal for next-generation optoelectronic and photocatalytic technologies due to their tunable optical properties and defect-tolerant structures. This review highlights recent breakthroughs in sustainable synthesis, including ligand-assisted reprecipitation and aqueous methods, reducing environmental impact by up to 50 % in terms of hazardous solvent usage and waste generation, based on life-cycle assessments comparing toxic organic solvents to greener alternatives. Advanced stabilization strategies—compositional engineering, surface passivation, and matrix encapsulation—enhance resilience against moisture, light, and heat, achieving photoluminescence quantum yield retention above 95 % after 30 days under stress conditions of 60 % relative humidity, 100 W cm⁻² UV light, and ambient temperature. Mechanistic insights from advanced characterization (e.g., XRD, XPS) elucidate defect dynamics and lattice stability, guiding further optimization. Future directions focus on green synthesis scalability and novel stabilization, such as self-healing ligands, to meet industrial demands. In addition, this review discusses techno-economic feasibility, regulatory compliance, and life-cycle assessment (LCA) as critical dimensions for sustainable commercialization. By embedding these perspectives alongside synthesis and stabilization strategies, the article provides a holistic roadmap for translating IHPQDs from laboratory breakthroughs into scalable, eco-friendly technologies.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 1","pages":"Article 100679"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-generation biosensing with perovskite quantum dots: From material engineering to rapid and multiplexed detection of infectious pathogens","authors":"Rima Heider Al Omari ,&nbsp;Ms.G. PadmaPriya ,&nbsp;Al-Hasnaawei Shaker ,&nbsp;Subhashree Ray ,&nbsp;Kattela Chennakesavulu ,&nbsp;Renu Sharma ,&nbsp;Ashish Singh Chauhan ,&nbsp;Nadia Sarhan","doi":"10.1016/j.cjac.2025.100677","DOIUrl":"10.1016/j.cjac.2025.100677","url":null,"abstract":"<div><div>Halide perovskite quantum dots (PQDs) enable sensitive detection of bacterial and viral pathogens in clinical, food, and environmental samples. Key technical advances include dual-mode lateral-flow assays combining fluorescence and electrochemiluminescence for Salmonella detection in milk and juice, lead-free Cs₃Bi₂Br₉-based photoelectrochemical sensors offering sub-femtomolar miRNA sensitivity with extended serum stability, and machine-learning-assisted fluorescent arrays achieving complete discrimination of multiple bacteria in tap water. Major challenges remain aqueous-phase degradation, lead-related toxicity of CsPbBr₃ PQDs, and regulatory barriers to clinical use. Although surface passivation extends stability for weeks, Pb²⁺ release from lead-based compositions typically exceeds permitted levels for parenteral administration, whereas bismuth-based PQDs already meet current safety standards without additional coating. Further progress requires scalable lead-free formulations, robust long-term stability under physiological conditions, and standardized validation protocols. Integration with portable detection systems, nucleic-acid amplification techniques, and microfluidic platforms is essential for practical point-of-care implementation. This review article systematically covers the intersection of PQD material engineering and biosensing for viral and bacterial detection.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 1","pages":"Article 100677"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145705629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of network pharmacology and experimental verification to reveal the active constituents and molecular mechanism of Compound Fujin Shengji (FJSJ) powder in the treatment of diabetes ulcer","authors":"Jing GAO ,&nbsp;Xiaohui DONG ,&nbsp;Hang LI ,&nbsp;Qing YANG ,&nbsp;Wenting JI ,&nbsp;Xianying LU ,&nbsp;Xinyu CHEN ,&nbsp;Fei WANG ,&nbsp;Chaoming HOU","doi":"10.1016/j.cjac.2025.100624","DOIUrl":"10.1016/j.cjac.2025.100624","url":null,"abstract":"<div><h3>Objective</h3><div>Fujin Shengji (FJSJ) Powder has a positive effect on healing diabetic ulcers (DU), but the specific compounds at work have not been identified.</div></div><div><h3>Methods</h3><div>Network pharmacology was employed to investigate the primary constituents, potential therapeutic targets, and pharmacological mechanisms of FJSJ powder treating DU. In vitro experiments were further performed to validate these findings: an LPS-induced RAW264.7 cell model was established to assess the anti-inflammatory effects of FJSJ Powder active constituents treating DU, while a high glucose-induced HUVEC model was cultured to verify the pro angiogenic effect of FJSJ powder active constituents treating DU. Additionally, the mechanisms by which the optimal active monomers suppress inflammation and enhance angiogenesis in DU were investigated, focusing on the JAK2/STAT3 and PI3K/AKT signaling pathways.</div></div><div><h3>Results</h3><div>Network pharmacology identified quercetin (Que), kaempferol (Kae), abietic acid (Aba) and acetyl-11-keto-<em>β</em>-boswellic acid (AKBA) as the primary active constituents of FJSJ Powder treating DU; IL6, TNF, AKT1, and VEGFA were the core targets; and anti-inflammatory effect was mainly enriched in the JAK2/STAT3 signaling pathway, while angiogenesis promotion was mainly enriched in the PI3K/AKT signaling pathway. The vitro experiments have confirmed Que, Kae, Aba, and AKBA could play important roles in anti-inflammatory and angiogenic effects, with AKBA and Aba playing important roles in anti-inflammatory and pro-angiogenic effects, respectively. AKBA can upregulate P-JAK2, P-STAT3, IL-4, and downregulate INOS protein levels to exert anti-inflammatory. Aba can upregulate the P-AKT, P-PI3K, HIF-1α, and VEGFA proteins levels to promote angiogenesis.</div></div><div><h3>Conclusion</h3><div>FJSJ powder promotes DU healing through dual-pathway regulation in anti-inflammatory and pro-angiogenic effects, with AKBA mediating the best anti-inflammatory activity through the JAK2/STAT3 pathway and Aba driving the strongest pro-angiogenic effects via the PI3K/AKT/HIF-1<em>α</em>/VEGFA axis, providing a basis for formula optimization.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 1","pages":"Article 100624"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of divinely benzene polymer on silica anchored graphene oxide for the efficient removal of acid yellow 25 and acid black 210","authors":"Musa KHAN,&nbsp;Faiz ALI,&nbsp;Mian MUHAMMAD","doi":"10.1016/j.cjac.2025.100622","DOIUrl":"10.1016/j.cjac.2025.100622","url":null,"abstract":"<div><div>The renovated Fischer esterification protocol was adopted for the chemical binding of silica particles on the surface of graphene oxide sheets. Divinely benzene polymer was attached to the silica@GO composite by the reversible addition fragmentation chain transfer polymerization. The polymer bound composite was characterized by Brunauer Emmett and Teller (BET/BJH) analysis, X-ray diffraction (XRD), zeta pH and Fourier transform infrared (FTIR) spectroscopy, then evaluated for the removal of acid yellow-25 and acid black-210 with promising results. During the batch adsorption studies maximum adsorption was recorded at pH 3 and 2 for acid yellow 25 and acid black-210, respectively. The qe value for the polymer bound composite towards acid black-210 was found 227.791 mg/g, while that of the acid yellow-25 it was found 113.896 mg/g. The adsorption data of both dyes was following the pseudo 2nd order kinetic model with regression values of 0.9989 and 0.9949 for acid yellow 25 and acid black 210 respectively. In adsorption isothermal study, the adsorption data of acid yellow 25 and acid black 210 is best fitting with the Freundlich model having <em>R</em>² value of 0.987 and 0.958 for acid black 210 and acid yellow 25, respectively.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 1","pages":"Article 100622"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic analysis for evaluating gut microbiome alterations in cardiac surgery-associated acute kidney injury","authors":"Yunpeng Bai ,&nbsp;Zhenmi Liu ,&nbsp;Yali Hu ,&nbsp;Yang Zhao ,&nbsp;Liwei Ren ,&nbsp;Xin Liu ,&nbsp;Silin Liang ,&nbsp;Linhui Hu ,&nbsp;Chunbo Chen ,&nbsp;Bing Xiong","doi":"10.1016/j.cjac.2025.100646","DOIUrl":"10.1016/j.cjac.2025.100646","url":null,"abstract":"<div><h3>Background</h3><div>Cardiac surgery-associated acute kidney injury (CSA_AKI) is linked to increased morbidity and mortality in intensive care unit (ICU) patients. Emerging evidence indicates a bidirectional interaction between CSA_AKI and gut microbiota dysbiosis. This exploratory study aimed to further investigate the potential relationships between CSA_AKI and alterations in the gut microbiota during disease progression.</div></div><div><h3>Methods</h3><div>A cohort of patients undergoing cardiac surgery was enrolled. Based on AKI diagnosis and prognosis, faecal samples were categorised into Non_AKI, CSA_AKI and Recovered_AKI groups. The taxonomic composition and functional pathways of the gut microbiota were profiled through shotgun metagenomic sequencing. The Shannon index and Bray–Curtis dissimilarity were calculated for <em>α</em>- and <em>β</em>-diversity assessment, respectively. Differentially abundant taxa and pathways were identified using linear discriminant analysis effect size and two-tailed Welch’s <em>t</em>-tests.</div></div><div><h3>Results</h3><div>Of the 39 patients, 19 (48.7 %) developed AKI. Abundance profiling revealed <em>Bacteroides, Prevotella</em> and <em>Escherichia</em> as the predominant genera across the groups, and a shift from strict anaerobes to facultative anaerobes was observed during CSA_AKI treatment. Pathway diversity was higher in the Recovered_AKI group than in the other groups. At the genus level, <em>Sellimonas</em> was enriched in the Non_AKI group, <em>Campylobacter</em> and <em>Streptococcus</em> in the CSA_AKI group and <em>Flavonifractor</em> in the Recovered_AKI group. Correlation analyses suggested that changes in functional pathways were associated with these differentially abundant genera.</div></div><div><h3>Conclusions</h3><div>CSA_AKI is accompanied by significant alterations in gut microbiota composition, and treatment does not restore the microbial community to a pre-AKI state. This exploratory investigation highlights potential gut microbiota biomarkers linked to CSA_AKI onset and progression, offering novel insights into its pathogenesis.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"54 1","pages":"Article 100646"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}