Chinese Journal of Analytical Chemistry最新文献

{"title":"Simulation study of a planar quadrupole mass filter for MEMS mass spectrometer","authors":"Yu-Peng CHENG ,&nbsp;Chen SHEN ,&nbsp;Zhang-Xu WU ,&nbsp;Shan LI ,&nbsp;You-Jiang LIU ,&nbsp;Han WANG ,&nbsp;Chi-Lai CHEN","doi":"10.1016/j.cjac.2024.100364","DOIUrl":"10.1016/j.cjac.2024.100364","url":null,"abstract":"<div><p>Micro-electro-mechanical system (MEMS) based mass spectrometry, MEMS mass spectrometry, emerges a new technique branch of miniature mass spectrometry. One of the main challenges hindering the development of MEMS mass spectrometry is the poor compatibility between the complex electrode structure of traditional ion optics and MEMS processes. This paper proposed a planar quadrupole mass filter utilizing a highly simplified stacked-layer structure and film electrodes, which is easy to fabricate with high precision by MEMS surface micromachining. The ion trajectory simulation model of the MEMS mass spectrometry chip involving all necessary ion optics was elaborated for investigating the preliminary performance of the planar quadrupole mass filter. By optimizing the crucial parameters such as the frequency of radio frequency voltage, the ratio of direct current voltage and radio frequency voltage, pre-pole length, main-pole length, and injection kinetic energy, the preliminary simulation result of planar quadrupole mass filter achieved a 1–2 Da peak width (at 50% of peak height) and 20–80% ion transmission efficiency. Importantly, the planar quadrupole mass filter demonstrated a maximum working pressure of 0.1 Pa (using air as the buffer gas), 100 times higher than that of conventional quadrupole mass filters, while maintaining a 0.7–1.8 Da peak width and ∼5% ion transmission efficiency. Additionally, to modify the theoretical formulas for the non-ideal quadrupolar field of planar quadrupole mass filter, a novel approach based on stability diagrams obtained by simulation was also introduced. The pretty good linearity (<em>R</em>²=1) between ion masses in the range of 10 to 100 Da and the corresponding radio frequency voltages was verified through the simulated mass spectra. Notably, the proportional coefficient of ion mass and radio frequency voltage exhibited a minimal 4% deviation between the simulated value (4.7132 V_0P/Da) and the calculated value (4.9036 V_0P/Da) through the modified formula. In conclusion, the preliminary simulation results demonstrated that the planar quadrupole mass filter possessed moderate performance, high-pressure tolerance, and excellent theoretical consistency, which proved it a promising technology of MEMS mass spectrometry. It can find applications in the fields of gas detection, including the rapid response to hazardous gas emissions and in situ detection of dissolved gases in the deep sea.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 2","pages":"Article 100364"},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000094/pdfft?md5=80a608cc2d09dd677411fd4a693206f6&pid=1-s2.0-S1872204024000094-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139588606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of volatile components of Panax ginseng flower by four extraction methods","authors":"Xin-Hong SONG ,&nbsp;Lu-Sheng HAN ,&nbsp;Lan YU ,&nbsp;Yang-Bin LAI ,&nbsp;En-Peng WANG ,&nbsp;Shu-Ying LIU","doi":"10.1016/j.cjac.2024.100366","DOIUrl":"10.1016/j.cjac.2024.100366","url":null,"abstract":"<div><p>Panax ginseng flowers (PGF), a constituent of ginseng, possess medicinal value and are widely recognized as a functional food. Therefore, ensuring meticulous control of PGF quality has become increasingly significant. One effective method for assessing product quality is to analyze the volatile organic compounds (VOCs) present in PGF, which act as indicative markers. In this study, VOCs in PGF were analyzed using gas chromatography-mass spectrometry (GC-MS), employing four extraction methods: steam distillation (SD), supercritical fluid extraction (SFE), headspace solid-phase microextraction (HS-SPME), and static headspace sampling (SHS). The results revealed 103 annotated components using the area normalization approach, highlighting the major differences among the four extraction methods. The main sesquiterpenoid components of PGF, 1,6,10-dodecatriene, 7,11-dimethyl-3-methylene-, and (<em>E</em>)- were the only common components. The SD, SFE, HS-SPME, and SHS methods identified 67, 20, 38, and 18 components, respectively. These components accounted for 89.67%, 61.84%, 82.6%, and 50.3%, respectively, of the total volatile oil effluent peak areas. Additionally, each method extracted 11, 9, 8, and 8 components, with proportions exceeding 1% in the PGF extracts. Terpenoids were identified as the main VOCs in PGF, with the SD method showing the highest sesquiterpene content. Both HS-SPME and SHS methods contained sesquiterpenoids, which accounted for 61.471% and 22.708% of the total relative amount, respectively. Furthermore, the extraction capacity of the HS-SPME method is approximately three times higher than that of the SHS method. The volatile oil extract of PGF consists of main components such as alkanes, esters, sesquiterpenes, and steroids, with compound contents exceeding 1%. Our findings provide a theoretical foundation for the rational development of PGF resources.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 2","pages":"Article 100366"},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000112/pdfft?md5=29c10691e3ff2529370bec89d2624b9d&pid=1-s2.0-S1872204024000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139689671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New approach for the inhibition of PCDD/F formation in fly ash by carbohydrazide during refuse-derived fuel (RDF) combustion","authors":"Shifeng Zhou ,&nbsp;Zhenzhen Guan ,&nbsp;Dezhen Chen ,&nbsp;Liu Hong","doi":"10.1016/j.cjac.2024.100357","DOIUrl":"10.1016/j.cjac.2024.100357","url":null,"abstract":"<div><p>PCDD/Fs in fly ash from municipal solid waste incineration (MSWI), pose significant health and environmental risks. Recent research has focused on using chemical inhibitors in the post-combustion zone to effectively inhibit PCDD/F formation. This study examined a new inhibitor, carbohydrazide (CHZ), by exploring its application techniques and mechanism for PCDD/F inhibition. A comparative analysis was conducted to identify the efficient CHZ addition method, by comparing the PCDD/F suppression effect by CHZ premixing and cofiring with refuse-derived fuel (RDF) and injecting into the flue gas. It was revealed that the premixing and cofiring of CHZ with RDF resulted in a 10.9% reduction in the formation of PCDD/Fs, demonstrating the effectiveness of the suppression of PCDD/F emissions. XRD analysis showed that the addition of CHZ led to the reduction of CuO to Cu₂O in bottom and fly ash, and thus deactivate the Cu-based catalysts. In addition, CHZ could also passivate the transition metal catalysts through coordination. Consequently, CHZ could be considered as a potential PCDD/F inhibitor during the combustion process.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 2","pages":"Article 100357"},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000021/pdfft?md5=d90a278d0700a7503fa67c79a9cfe973&pid=1-s2.0-S1872204024000021-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139509010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Incorporation of metal-organic framework MOFs-5 into the polymer monolith via the surface covalent immobilization method for enhanced capillary liquid chromatographic separation of benzene homologues” [Chinese Journal of Analytical Chemistry 51 (2023) 100199]","authors":"","doi":"10.1016/j.cjac.2023.100355","DOIUrl":"10.1016/j.cjac.2023.100355","url":null,"abstract":"","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 1","pages":"Article 100355"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023001299/pdfft?md5=0d22541c5f0660a5d4cd99f6a221b0ee&pid=1-s2.0-S1872204023001299-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adsorption of ciprofloxacin on co-pyrolyzed biochar from fish scale and pine needle","authors":"Xiaoxiao LU,&nbsp;Jingchan ZHAO","doi":"10.1016/j.cjac.2023.100350","DOIUrl":"10.1016/j.cjac.2023.100350","url":null,"abstract":"<div><p>The biochar (FS-PNBC) is prepared by pyrolyzing mixed biomass of fish scale and pine needle in varied proportions in a tube furnace. The biochar was characterized by a specific Brunauer-Emmett-Teller (BET) surface area analyzer, scanning electron microscope (SEM), Fourier infrared spectrometer (FTIR), and X-ray diffractometer (XRD). In a solution with an initial concentration of 60 mg/L, the adsorption of ciprofloxacin (CIP) by a 1:1 ratio of fish scale and pine needle co-pyrolyzed biochar (FS-PNBC1) reached 27.97 mg/g. The adsorption properties such as thermodynamics, kinetics, isothermal adsorption, and adsorption factors were investigated by batch experiments. The results indicate that FS-PNBC exhibits a significantly large specific surface area, abundant pore structure, and a high level of porosity. After approximately 180 min, adsorption equilibration was achieved in a pH=4 solution. The kinetic data was well-matched with a quasi-second-order model, suggesting that chemisorption plays a role in the adsorption process. Isothermal adsorption and thermodynamic results indicate that the adsorption of CIP by biochar is more consistent with the Freundlich model, which suggests multilayered non-homogeneous phase adsorption and an exothermic entropy-increasing reaction capable of proceeding spontaneously. FS-PNBC contains oxygen-containing functional groups, and through FTIR analysis, it was observed that hydrogen bonding, π-π bonding, and electrostatic interaction are closely linked to adsorption. This study demonstrates that biochar produced through the co-pyrolysis of fish scale and pine needle has the potential to serve as an efficacious adsorbent for extracting ciprofloxacin from wastewater.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 1","pages":"Article 100350"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S187220402300124X/pdfft?md5=19c7f205a9fd42bc1c673027f51243a2&pid=1-s2.0-S187220402300124X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138691553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and properties of metakaolin-fumed-silica geopolymer modified with sodium silicate and potassium silicate activators","authors":"Guoya JIN ,&nbsp;Xiaofei WANG ,&nbsp;Huimin MAO ,&nbsp;Shidong JI ,&nbsp;Qiao SHI","doi":"10.1016/j.cjac.2023.100352","DOIUrl":"10.1016/j.cjac.2023.100352","url":null,"abstract":"<div><p>Geopolymers, which are environmentally friendly materials with aluminosilicates as raw materials, are expected to replace cement in construction materials. Here, the effect of fumed silica (FS) on the properties of metakaolin (MK)-based geopolymer materials was investigated with MK, sodium silicate, and potassium silicate as the main raw materials; their microstructures were characterized, and their performance was tested. The geopolymer's compressive strength, particle size, and curing characteristics were measured with X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy for microstructural analysis. The total dissolved solids (TDS) method was employed to study the geopolymer and evaluate the effect of the resulting gel products on TDS. The results revealed that the strength of 4% FS could reach 56.3MPa after 7 days of incorporation, and the strength increased by 20% and 12% compared with the 0% and 2% groups, respectively. Meanwhile, studying the microstructure of kaolin revealed that the best mechanical and physical properties were obtained when kaolin was calcined at 700°C. In addition, the mixture of alkaline activators increased the reactivity of MK, and the mixed alkaline-excited geopolymer from sodium silicate and potassium silicate exhibited enhanced matrix strength properties compared to the single alkaline activators. Overall, the inclusion of the FS material resulted in the optimization of the mechanical properties, confirming the improvement in the mechanical properties.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 1","pages":"Article 100352"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023001263/pdfft?md5=e66b619a1430efcff08995e4e9a74e32&pid=1-s2.0-S1872204023001263-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138825145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the effect of gut-joint axis dysfunction on rheumatoid arthritis and the intervention of natural products","authors":"Wentao GUO,&nbsp;Guangfu LV,&nbsp;Di YANG,&nbsp;Wenjing ZHANG,&nbsp;Nian LI,&nbsp;Jiannan HU,&nbsp;Yongxi WU,&nbsp;Zifeng PI,&nbsp;Zhe LIN","doi":"10.1016/j.cjac.2023.100354","DOIUrl":"10.1016/j.cjac.2023.100354","url":null,"abstract":"<div><p>Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory malady that predominantly affects the joints. Emerging evidence highlights the perturbation of intestinal microflora and its resultant metabolites as potential instigators of disruptions within the \"gut-joint axis\". These disruptions subsequently trigger localized or systemic immune responses, culminating in joint inflammation and consequent impairment. While the efficacy of natural compounds in managing RA has been increasingly recognized, the precise mechanisms underlying their actions within the 'gut-joint axis' remain incompletely elucidated. The primary objective of this comprehensive investigation is to assess the effects of the intestinal barrier, gut microbiota, and metabolites on the pathogenesis of RA. Moreover, it aims to delineate the mechanisms through which natural products exert their therapeutic effects on the treatment of RA, all within the framework of the 'gut-joint axis'.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 1","pages":"Article 100354"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023001287/pdfft?md5=6eb59585024e139a904f74b4759cf5e4&pid=1-s2.0-S1872204023001287-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining network pharmacology and molecular docking to decipher molecular mechanism of Wugen decoction in influenza A prevention","authors":"Hao-Ran Fan ,&nbsp;Chen-Xi Ren ,&nbsp;Fan Shi ,&nbsp;Li-Jun Meng ,&nbsp;Gang-Hui Chu ,&nbsp;Abdulla Yusuf ,&nbsp;Tian-Zhu Guan ,&nbsp;Hai-Feng Zhang","doi":"10.1016/j.cjac.2023.100353","DOIUrl":"10.1016/j.cjac.2023.100353","url":null,"abstract":"<div><p>Wugen decoction, a classic traditional Chinese medical diet, is widely used to prevent influenza A virus infection in China. However, the principal effective ingredients and potential mechanism of Wugen decoction remain unclear. Therefore, a systemic analysis-based approach combined with molecular docking and network pharmacology is used to elucidate the molecular mechanism of Wugen decoction in prevention of influenza A. The efficient bioactive and potential targets of Wugen decoction are initially retrieved from public databases and relevant references. To further analyze the core targets, a protein-protein interaction network is investigated through cluster screening, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, and Gene Ontology analysis. Then the binding behaviors between crucial bioactive ingredients and core targets are obtained via molecular docking. Quercetin, stigmasterol, beta-sitosterol, genistein, oxalic acid, and daidzein with higher degrees are collected and screened from the “Herb-Ingredients” network. Meanwhile, AKT1, IL1B, IL6, INS, TNF, and VEGFA are identified from cluster screening and topological investigation. The coincident targets are primarily concentrated to biological processes including hydrogen peroxide, cellular response to synapse, and molecular function to cysteine-type endopeptidase activity involved in tumor necrosis factor receptor binding. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis manifests that the signaling pathways related to influenza A prevention are closely associated with tumor necrosis factor receptor binding, RNA polymerase II sequence-specific DNA binding transcription factor binding, and protein phosphatase binding. Molecular docking results highlight that the effective ingredients can spontaneously bind with the core targets. In all, the present research comprehensively illustrates the potential mechanism of Wugen decoction against influenza A virus infection with the ‘multicomponent-multitarget-multipathway’ mode. This research also provides an effective approach to uncover the scientific basis of Wugen decoction within food and health care industries.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 2","pages":"Article 100353"},"PeriodicalIF":1.2,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023001275/pdfft?md5=87fccd2a37c5051d7f1cf0d130cb449d&pid=1-s2.0-S1872204023001275-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138825500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanism of tenghuang jiangu wan in osteoporosis treatment based on network pharmacology, molecular docking and experimental pharmacology","authors":"Wenjing ZHANG ,&nbsp;Mingyang SUN ,&nbsp;Guangfu LV ,&nbsp;Wentao GUO ,&nbsp;Jiannan HU ,&nbsp;Jingye GU ,&nbsp;Yuchen WANG ,&nbsp;Qing GONG ,&nbsp;Zifeng PI ,&nbsp;Zhe LIN","doi":"10.1016/j.cjac.2023.100351","DOIUrl":"10.1016/j.cjac.2023.100351","url":null,"abstract":"<div><p>In this study, we employed network pharmacology and molecular docking techniques, complemented by experimental validation, to assess the pharmacodynamic effects of tenghuang jiangu wan (THJGW) in the context of osteoporosis (OP) treatment. Our investigation delineated the underlying mechanism of action. To simulate clinical postmenopausal osteoporosis, we used a rat model of bilateral ovary removal. Through assessment of bone mineral density, bone microstructure, histological examination of bone tissues and evaluation of biochemical markers, it was confirmed that THJGW can effectively increase the number of bone trabeculae in ovx rats and improve structural damage. In addition, it has a significant protective effect on bone turnover, thereby demonstrating its therapeutic potential for OP. Furthermore, using bioinformatics analysis, we predicted that THJGW could modulate various processes <em>in vivo</em>. These include the regulation of inflammatory responses, growth factors, steroid hormone levels, biosynthetic pathways, and effects on lipids and atherosclerosis. Additionally, we investigated its role in the lipid and atherosclerosis signaling pathway. By activating the Wnt/β-catenin signaling pathway and concurrently inhibiting the Axin2/PPAR-γ signaling pathway, THJGW promotes the proliferation and differentiation of bone marrow mesenchymal stem cells into osteoblasts, reduces adipocyte production, and enhances bone formation. This dual mechanism underscores their preventive and therapeutic potential against OP. This study provides valuable scientific insights and a robust theoretical foundation for the clinical use of THJGW.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 1","pages":"Article 100351"},"PeriodicalIF":1.2,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023001251/pdfft?md5=1c0bb34d9edaced76808fa8b91f6bcd8&pid=1-s2.0-S1872204023001251-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138691932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying deer-hide gelatin of Tarim red deer (Cervus elaphus yarkandensis) through active peptide analysis","authors":"Xinxin BAO ,&nbsp;Xuenan CHEN ,&nbsp;Ying CHEN ,&nbsp;Chunjie HU ,&nbsp;Xiaowei HUANG ,&nbsp;Yunting ZHAO ,&nbsp;Chao XU ,&nbsp;Liwei SUN ,&nbsp;Qingjie LI","doi":"10.1016/j.cjac.2023.100349","DOIUrl":"10.1016/j.cjac.2023.100349","url":null,"abstract":"<div><p>Deer hide gelatin (DHG) is a food and pharmaceutical product derived from deer hide. However, the identifying the subspecies origin of red deer in the DHG still poses challenges. This study describes a novel approach for identifying DHG derived from Tarim red deer. It employs labeled peptides specific to species-specific proteins found in Tarim red deer. Species-specific proteins of Tarim red deer were identified and validated using proteomic techniques. A peptide derived from a specific protein of Tarim red deer was synthesized and employed for the development of the liquid chromatography multiplex reaction monitoring mass spectroscopy method. The capability of this method was evaluated using homemade gelatin for testing purposes. Proteomic analysis revealed the presence of a diverse range of proteins in the skin of the Tarim red deer, including Ig-like and serpin domain-containing proteins. The peptide derived from the species-specific Ig-like domain-containing protein of Tarim red deer demonstrated exceptional specificity towards DHG. Standard Pep-1 solutions were examined at a concentration of 1 × 10⁻⁴ mg/mL using liquid chromatography multiplex reaction monitoring mass spectrometry. DHG from Tarim red deer could be accurately detected using this technology. The effectiveness of the synthetic Tarim red deer-specific peptide in enhancing motor function and neuromuscular coordination was confirmed through assessment of glutathione content and superoxide dismutase activity, as well as Drosophila climbing assay and \"Smurf test\". The method developed in this study could improve the quality control of DHG from Tarim red deer and the development of health products and could also be applied to the identification of other foods and drugs containing protein components.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 1","pages":"Article 100349"},"PeriodicalIF":1.2,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023001238/pdfft?md5=791cdeba9600de27d349af18e3e76ee2&pid=1-s2.0-S1872204023001238-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138530662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}