Renal protective effect of Isaria felina mycelium powder on diet and STZ-induced diabetes mice and the identification of major chemical constituents

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2024-11-01 DOI:10.1016/j.cjac.2024.100451

Ziyang HUANG , Jingjing CHEN , Lili ZHAO , Lixia CHEN , Yuxin YANG , Junjun LI , Jing WANG , Yongming YANG , Lei YAN , Guoyan YU , Xiaowei SHI , Yiyin ZHANG , Yanlin YU , Sujiao LIANG , Xihua YANG

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引用次数: 0

Abstract

Type 2 diabetes is a group of metabolic diseases mainly characterized by chronic hyperglycemia. Diabetic kidney disease is the most common microvascular complication of diabetes.

The aim of this study was to investigate the therapeutic effect of Isaria felina mycelium powder (IFMP) on diet and streptozotocin (STZ)-induced diabetic mouse model. And we analyze the main chemical constituents of Isaria felina mycelium powder.

The main chemical constituents of the alcohol-soluble IFMP were prepared and identified by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). The fasting blood glucose and renal index of the mouse model was determined. Serum levels of triglyceride (TG), total cholesterol (TC), serum creatinine (Scr) and blood urea nitrogen (BUN) were detected. The levels of CD2AP and nephrin proteins in the kidney were detected by ELISA kits.

A total of 32 compounds were identified, including nucleosides (16 types), amino acids (13 types), sterols (one type) and glycosides (two types). The experimental results showed that compared with the diabetes model group, IFMP reduced fasting blood glucose, food intake and water intake. Biochemical analysis indicated that IFMP reduced the serum levels of TG, TC, BUN and Scr, and increased the levels of CD2AP and nephrin proteins in the kidneys.

Therefore, IFMP has the potential to treat diabetes and prevent kidney damage induced by hyperglycemia.

Abstract Image

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鱼腥草菌丝粉对饮食和 STZ 诱导的糖尿病小鼠的肾脏保护作用及主要化学成分的鉴定

2型糖尿病是一组以慢性高血糖为主要特征的代谢性疾病。本研究旨在探讨刺五加菌丝体粉（IFMP）对饮食和链脲佐菌素（STZ）诱导的糖尿病小鼠模型的治疗效果，并分析刺五加菌丝体粉的主要化学成分。采用高效液相色谱-四极杆飞行时间质谱法（HPLC-Q-TOF-MS）制备并鉴定了醇溶性IFMP的主要化学成分。测定了小鼠模型的空腹血糖和肾脏指数。检测了血清甘油三酯（TG）、总胆固醇（TC）、血清肌酐（Scr）和血尿素氮（BUN）的水平。共鉴定出 32 种化合物，包括核苷类（16 种）、氨基酸类（13 种）、甾醇类（1 种）和苷类（2 种）。实验结果表明，与糖尿病模型组相比，IFMP 降低了空腹血糖、进食量和饮水量。生化分析表明，IFMP降低了血清中TG、TC、BUN和Scr的水平，提高了肾脏中CD2AP和肾素蛋白的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.