A highly selective and sensitive carbazole-based fluorescent probe for peroxynitrite detection and cellular imaging

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2024-11-01 DOI:10.1016/j.cjac.2024.100456

Said ALAM , Linchuan LU , Xuanzuo TAO , Yanxia MAO , Shaojun ZHENG , Chunhui JIANG , Shu-Yang CHEN , Hongfei LU

{"title":"A highly selective and sensitive carbazole-based fluorescent probe for peroxynitrite detection and cellular imaging","authors":"Said ALAM , Linchuan LU , Xuanzuo TAO , Yanxia MAO , Shaojun ZHENG , Chunhui JIANG , Shu-Yang CHEN , Hongfei LU","doi":"10.1016/j.cjac.2024.100456","DOIUrl":null,"url":null,"abstract":"<div><div>Peroxynitrite, a colorless and odorless reactive nitrogen species, plays a critical role in various physiological processes within biological systems. Therefore, developing a reliable method for quantitative detection of peroxynitrite in living systems is essential. In this study, we synthesized a simple, two-step, reaction-based fluorescent probe, designated as <strong>EHMC</strong>, specifically for peroxynitrite detection. The synthesis involved the use of 4-(9-phenyl-9H-carbazol-3-yl)benzaldehyde as the fluorophore and 2-hydrazineylpyridine as the recognition group, both of which exhibit inherently weak fluorescence. Upon oxidation of the C = N bond by peroxynitrite, <strong>EHMC</strong> undergoes conversion into an aldehyde, resulting in a significant fluorescence response. <strong>EHMC</strong> demonstrated high selectivity and sensitivity, achieving a detection limit of 5.43 µM under physiological conditions. Additionally, <strong>EHMC</strong> was successfully applied to HeLa cellular models, where it exhibited excellent cellular imaging capability for peroxynitrite detection in living systems.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 11","pages":"Article 100456"},"PeriodicalIF":1.2000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1872204024001014","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Peroxynitrite, a colorless and odorless reactive nitrogen species, plays a critical role in various physiological processes within biological systems. Therefore, developing a reliable method for quantitative detection of peroxynitrite in living systems is essential. In this study, we synthesized a simple, two-step, reaction-based fluorescent probe, designated as EHMC, specifically for peroxynitrite detection. The synthesis involved the use of 4-(9-phenyl-9H-carbazol-3-yl)benzaldehyde as the fluorophore and 2-hydrazineylpyridine as the recognition group, both of which exhibit inherently weak fluorescence. Upon oxidation of the C = N bond by peroxynitrite, EHMC undergoes conversion into an aldehyde, resulting in a significant fluorescence response. EHMC demonstrated high selectivity and sensitivity, achieving a detection limit of 5.43 µM under physiological conditions. Additionally, EHMC was successfully applied to HeLa cellular models, where it exhibited excellent cellular imaging capability for peroxynitrite detection in living systems.

Abstract Image

查看原文本刊更多论文

一种高选择性、高灵敏度的咔唑基荧光探针，用于过亚硝酸盐检测和细胞成像

过亚硝酸盐是一种无色无味的活性氮物种，在生物系统的各种生理过程中发挥着至关重要的作用。因此，开发一种可靠的方法来定量检测生命系统中的过氧化亚硝酸盐至关重要。在这项研究中，我们合成了一种简单的、基于两步反应的荧光探针，命名为 EHMC，专门用于过亚硝酸盐的检测。在合成过程中，我们使用 4-（9-苯基-9H-咔唑-3-基）苯甲醛作为荧光团，2-肼基吡啶作为识别基团。当 C = N 键被过氧化亚硝酸盐氧化时，EHMC 会转化为醛，从而产生明显的荧光反应。EHMC 具有很高的选择性和灵敏度，生理条件下的检测限为 5.43 µM。此外，EHMC 还成功地应用于 HeLa 细胞模型，在活体系统中进行过亚硝酸盐检测时，EHMC 表现出卓越的细胞成像能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.