Zhongguo yao li xue bao = Acta pharmacologica Sinica最新文献_第10页

Dihydroetorphine-induced place preference was mediated by dopamine D1 receptors in rats. 多巴胺D1受体介导双氢乙托啡诱导的大鼠位置偏好。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

Z H Liu, K G Zhang

引用次数: 0

Effects of 7-nitroindazole on long-term potentiation induced by l-clausenamide and high-frequency stimulation in rat hippocampus in vivo. 7-硝基吲唑对l-clausenamide和高频刺激诱导大鼠海马长时程增强的影响。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

M R Zhao, J T Zhang

引用次数: 0

Inhibition by baclofen of NMDA-activated current in rat dorsal root ganglion neurons. 巴氯芬对大鼠背根神经节神经元nmda激活电流的抑制作用。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

J Q Si, Z W Li

引用次数: 0

Characterization of outward potassium current in embryonic chick heart cells. 胚胎鸡心脏细胞外向钾电流的表征。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

Y A Mei, L X Huang, H Wei, J T Sun, H Q Zhou, Z H Zhang

{"title":"Characterization of outward potassium current in embryonic chick heart cells.","authors":"Y A Mei, L X Huang, H Wei, J T Sun, H Q Zhou, Z H Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To characterize a voltage-dependent outward K+ current in cultured heart cells of 14-16-day-old embryos of yellow chick.Methods: The patchclamp technique in the whole-cell configuration was used.Results: The kinetics and the pharmacology of the outward K+ current in our cell mold were different from those described in white chick. Like the calcium-activated K+ current, blocker of calcium channel, CdCl2, eliminated the current of more than 95%. Isoproterenol provoked an increase of peak amplitude (137% +/- 47%, n = 16 cells) and acceleration of activation kinetics in the outward K+ current (the time reaching a peak current reduced from 36 ms +/- 10 ms to 16 ms +/- 9 ms). This effect of isoproterenol was mimiced by cAMP. In addition, a frequency-dependent decrease in peak amplitude of the current occurred after cAMP-induced phosphorylation.Conclusion: There are species- and/or cell-type-specific difference in the K+ channels properties. In embryonic yellow chick heart cells, the phospholation of channel could not only modulate the activation kinetic properties of the calcium-activated potassium channel, but also change their recovery kinetics.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21316344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraventricular vascular endothelial growth factor antibody increases infarct volume following transient cerebral ischemia. 脑室内血管内皮生长因子抗体增加短暂性脑缺血后的梗死体积。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

W L Bao, S D Lu, H Wang, F Y Sun

{"title":"Intraventricular vascular endothelial growth factor antibody increases infarct volume following transient cerebral ischemia.","authors":"W L Bao, S D Lu, H Wang, F Y Sun","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To clarify the role of vascular endothelial growth factor (VEGF) in neuronal damage induced by cerebral ischemia.Methods: Expression of VEGF in adult rat brain was measured by immunohistochemistry. Transient middle cerebral artery occlusion (MCAO) model was induced by placing a nylon thread in the lumen of the internal carotid artery. The infarct volume was shown with 2,3,5-triphenyltetrazolium chloride (TTC) staining and quantitated by computer image analyzer with and without VEGF antibody treatment.Results: VEGF expression was widely distributed in neuronal cells besides vascular endothelial cells, and the neuronal distribution of VEGF was specific. After intraventricular treatment with VEGF antibody (0.1 g.L-1 daily, for 7 d following the ischemia), infarct volume in the antibody treatment was increased versus vehicle-treated rats [(21.6 +/- 2.7 vs 16 +/- 6) mm3, P < 0.05] respectively.Conclusion: Intraventricular injection of VEGF antibody increased the infarct volume after focal cerebral ischemia in rats, suggesting that expression of neuronal VEGF may be one of neuronal protective mechanisms.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21316345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Demethylation capacity of human fetal adrenal mitochondrial cytochrome P-450 in vitro. 人胎儿肾上腺线粒体细胞色素P-450体外去甲基化能力的研究。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

H Wang, R X Peng, Y H Zhang, J H Chen, Q X Li, R Kong, H Ding, J P Yu

{"title":"Demethylation capacity of human fetal adrenal mitochondrial cytochrome P-450 in vitro.","authors":"H Wang, R X Peng, Y H Zhang, J H Chen, Q X Li, R Kong, H Ding, J P Yu","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To explore the capacity and characteristics of adrenal mitochondria to metabolize xenobiotics in vitro in human fetus.Methods: Subcellular fractions of fetal adrenal were prepared by differential centrifugation. Mitochondrial P-450 system was proved by spectral analyses and SDS-PAGE. The formaldehyde formation contents were measured with Nash reagent.Results: The erythromycin N-demethylation linearly increased in the protein concentration (1-4 mg)- and incubation time (10-30 min)-dependent manners. A typical concentration-effect relationship appeared with erythromycin 0.067-1 mmol.L-1 and a positive correlation (r = 0.641, P < 0.05) existed between erythromycin N-demethylation and gestation months. The N-demethylation values (nmol.s-1/g protein) of erythromycin (2.7 +/- 0.8), benzfetamine (1.1 +/- 0.5), and aminophenazone (0.9 +/- 0.4) in mitochondria were 89% (P > 0.05), 162% (P < 0.01), and 62% (P < 0.01), respectively, of those in microsomes. There was correlation between mitochondria and microsomes in the N-demethylation of erythromycin (r = 0.708, P < 0.05) and benzfetamine (r = 0.707, P < 0.05). Troleandomycin stimulated erythromycin N-demethylation in adrenal mitochondria as well as in adrenal and liver microsomes in vitro.Conclusion: Fetal adrenal mitochondria, with multiple P-450 isoforms and greater capacity of demethylation, play a role in drug-metabolism during fetal development.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21316348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theoretical elucidation of activity differences of five phenolic antioxidants. 五种酚类抗氧化剂活性差异的理论解释。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

H Y Zhang, N Ge, Z Y Zhang

{"title":"Theoretical elucidation of activity differences of five phenolic antioxidants.","authors":"H Y Zhang, N Ge, Z Y Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To verify the effectiveness of structure-activity relationship (SAR) and theoretical calculation methods for antioxidants.Methods: Preliminary elucidation on the differences of activities of 5 antioxidants was performed by SAR. Then semiempirical quantum chemistry method AM1 was employed to calculate the delta HOF value, the difference between the heat of formation of antioxidant and its free radical, which was used as a theoretical parameter to elucidate the differences of activities of the antioxidants thoroughly.Results: delta HOF values of antioxidants were obtained as follows: ferulic acid, 150.58 kJ.mol-1; anion of ferulic acid, 122.64 kJ.mol-1; modified ferulic acid, 137.70 kJ.mol-1; anion of modified ferulic acid, 118.99 kJ.mol-1; salvianic acid, 134.17 kJ.mol-1; rutin, 137.83 kJ.mol-1, L-EGCG, 124.39 kJ.mol-1; paeonol, 176.79 kJ.mol-1. The differences of the antioxidant activities were elucidated, and how to further enhance the antioxidant activity was investigated as well.Conclusion: The SAR and calculation methods are rather effective to elucidate the differences of antioxidant activities, and present some new clues for structural modification of antioxidants to increase their activities.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21316349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between inhibitions of morphine withdrawal and nitric-oxide synthase by agmatine. 胍丁胺抑制吗啡戒断与一氧化氮合酶的关系。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

J Li, X Li, G Pei, B Y Qin

引用次数: 0

Relationship between adenosine-induced vascular effects and ATP-sensitive K+ channels. 腺苷诱导的血管效应与atp敏感K+通道的关系。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-03-01

H M He, H Wang, W B Xiao

{"title":"Relationship between adenosine-induced vascular effects and ATP-sensitive K+ channels.","authors":"H M He, H Wang, W B Xiao","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the relationship between adenosine (Ade) receptors and adenosine 5'-triphosphate (ATP)-sensitive potassium (KATP) channels in rat aorta.Methods: Isolated rat aorta rings were suspended for isometric force recording. The vascular effects of Ade were assessed in the presence or absence of functional endothelium. The interactions of Ade and pinacidil (Pin) or glibenclamide (Gli) were investigated.Results: In isolated aorta preconstricted with KCl 20 mmol.L-1, Ade 3-300 mumol.L-1 induced relaxation in a concentration-dependent manner; and in 48/99 preparations from 32 rats, Ade induced initial transient constriction followed by sustained relaxation. When the functions of KATP channels were blocked with Gli 1 or 100 mumol.L-1, effects of Ade were characterized by vasoconstriction rather than vasorelaxation. The combination of Pin 1 mumol.L-1 with Ade 100 mumol.L-1 showed no synergic vasodilatory effects and did not affect Ade-induced vasoconstriction. After the removal of endothelium, Ade still induced vasoconstriction and vasorelaxation, and the constrictive effects showed no difference from those in the presence of endothelium, but the potency of vasodilatory effects became weaker with slower decrease in tension.Conclusion: The activation of KATP channels is involved in Ade receptor-induced vasodilation.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21316414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basic fibroblast growth factor enhanced LAK cell cytotoxicities against human bladder neoplasm cells. 碱性成纤维细胞生长因子增强LAK细胞对人膀胱肿瘤细胞的细胞毒性。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-03-01

Z P Wang, G L Duan, Y R Chen, D S Qin, G D Liu, H G Niu

{"title":"Basic fibroblast growth factor enhanced LAK cell cytotoxicities against human bladder neoplasm cells.","authors":"Z P Wang, G L Duan, Y R Chen, D S Qin, G D Liu, H G Niu","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the effects of basic fibroblast growth factor (bFGF) on the proliferation of lymphokine-activated killer (LAK) cells from patients with bladder cancer and LAK cells cytolysis against bladder tumor cells.Methods: LAK cell proliferation was assayed in the presence of various concentrations of bFGF combined with interleukin-2 (IL-2) by cell count. Cytotoxicity of LAK cells against bladder cancer cell line EJ cells and bladder tumor cells (BTC) from patients was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays.Results: The proliferation of peripheral blood monocytes (PBMC) was inhibited by bFGF 5 micrograms.L-1. bFGF did not affect the stimulation of LAK cells induced by IL-2. The LAK cell numbers in the combination of IL-2 with bFGF were not significantly different compared with that treated with IL-2 alone. bFGF enhanced cytotoxicity of LAK cells against bladder cancer cell line EJ cells or BTC, respectively.Conclusion: Although the proliferation of PBMC was inhibited by bFGF, bFGF increased LAK cell cytotoxicity against bladder neoplasm cells.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21316418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0