{"title":"巴氯芬对大鼠背根神经节神经元nmda激活电流的抑制作用。","authors":"J Q Si, Z W Li","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>To explore the modulatory effect of baclofen on NMDA-activated current in rat dorsal root ganglion (DRG) neurons.</p><p><strong>Methods: </strong>Whole-cell patch-clamp technique was used to record NMDA-activated current in isolated DRG neurons. Drugs were applied by rapid solution exchange.</p><p><strong>Results: </strong>Preapplication of baclofen 1-100 mumol.L-1 induced a concentration-dependent inhibition of the inward NMDA-activated current markedly. NMDA (100 mumol.L-1)-activated current was inhibited by 52% +/- 14% (n = 11, P < 0.01) by preapplication of baclofen 100 mumol.L-1. The inhibitory effect of baclofen was reversible, and was removed by saclofen 100 mumol.L-1, which was a selective antagonist of GABAB receptor.</p><p><strong>Conclusion: </strong>Preapplication of baclofen exerts an inhibitory effect on NMDA-activated current in the primary sensory neurons.</p>","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inhibition by baclofen of NMDA-activated current in rat dorsal root ganglion neurons.\",\"authors\":\"J Q Si, Z W Li\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>To explore the modulatory effect of baclofen on NMDA-activated current in rat dorsal root ganglion (DRG) neurons.</p><p><strong>Methods: </strong>Whole-cell patch-clamp technique was used to record NMDA-activated current in isolated DRG neurons. Drugs were applied by rapid solution exchange.</p><p><strong>Results: </strong>Preapplication of baclofen 1-100 mumol.L-1 induced a concentration-dependent inhibition of the inward NMDA-activated current markedly. NMDA (100 mumol.L-1)-activated current was inhibited by 52% +/- 14% (n = 11, P < 0.01) by preapplication of baclofen 100 mumol.L-1. The inhibitory effect of baclofen was reversible, and was removed by saclofen 100 mumol.L-1, which was a selective antagonist of GABAB receptor.</p><p><strong>Conclusion: </strong>Preapplication of baclofen exerts an inhibitory effect on NMDA-activated current in the primary sensory neurons.</p>\",\"PeriodicalId\":24002,\"journal\":{\"name\":\"Zhongguo yao li xue bao = Acta pharmacologica Sinica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo yao li xue bao = Acta pharmacologica Sinica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:探讨巴氯芬对大鼠背根神经节神经元nmda激活电流的调节作用。方法:采用全细胞膜片钳技术记录离体DRG神经元的nmda激活电流。采用快速换液给药。结果:巴氯芬1- 100mumol预应用。L-1对nmda激活电流有明显的浓度依赖性抑制作用。预施用百氯芬100 mummol . l -1可抑制NMDA (100 mummol . l -1)激活电流52% +/- 14% (n = 11, P < 0.01)。巴氯芬的抑制作用是可逆的，可被100 μ mol的氯芬所消除。L-1，是GABAB受体的选择性拮抗剂。结论:巴氯芬预施对初级感觉神经元nmda激活电流有抑制作用。
Inhibition by baclofen of NMDA-activated current in rat dorsal root ganglion neurons.
Aim: To explore the modulatory effect of baclofen on NMDA-activated current in rat dorsal root ganglion (DRG) neurons.
Methods: Whole-cell patch-clamp technique was used to record NMDA-activated current in isolated DRG neurons. Drugs were applied by rapid solution exchange.
Results: Preapplication of baclofen 1-100 mumol.L-1 induced a concentration-dependent inhibition of the inward NMDA-activated current markedly. NMDA (100 mumol.L-1)-activated current was inhibited by 52% +/- 14% (n = 11, P < 0.01) by preapplication of baclofen 100 mumol.L-1. The inhibitory effect of baclofen was reversible, and was removed by saclofen 100 mumol.L-1, which was a selective antagonist of GABAB receptor.
Conclusion: Preapplication of baclofen exerts an inhibitory effect on NMDA-activated current in the primary sensory neurons.
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