Zhongguo yao li xue bao = Acta pharmacologica Sinica最新文献

Cytokines enhance nitric oxide production from human BT325 astrocytoma cells. 细胞因子促进人BT325星形细胞瘤细胞产生一氧化氮。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

H W Zhao, X Y Li

引用次数: 0

Supraspinal D2 receptor involved in antinociception induced by l-tetrahydropalmatine. 脊髓上D2受体参与l-四氢巴马汀诱导的抗痛觉作用。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

J Y Hu, G Z Jin

{"title":"Supraspinal D2 receptor involved in antinociception induced by l-tetrahydropalmatine.","authors":"J Y Hu, G Z Jin","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the role of dopamine (DA) receptors in l-tetrahydropalmatine (l-THP)-induced antinociception.Methods: The intraperitoneal (i.p.) and intrathecal (ith) injections were used to give the drugs. The tail-flick test was used to assess the nociceptive threshold of rats.Results: By i.p. injection, l-THP (10, 20, 40 mg.kg-1) as well as D2 receptor antagonist spiperone (1, 2, 3 mg.kg-1) produced dose-dependent antinociceptive effects on the nociception of rats, while D2 receptor agonist quinpirole, D1 receptor agonist SKF38393, and D1 receptor antagonist Sch-23390 showed no antinociception. Moreover, l-THP- or spiperone-induced antinociception was markedly attenuated by quinpirole (2, 3 mg.kg-1) but not SKF38393 or naloxone. On the other hand, ith quinpirole (20, 30, 40 micrograms.kg-1) also induced a dose-dependent antinociception, while ith l-THP, spiperone, SKF38393, and Sch-23390 did not exhibit any antinociception. Furthermore, ith spiperone (20, 30, 40 micrograms.kg-1) but not Sch-23390 dose-dependently antagonised the antinociception induced by quinpirole. l-THP (ith, 100, 200, 300 micrograms.kg-1) also dramatically attenuated the quinpirole-induced antinociception with a dose-dependent relationship.Conclusion: Activating the spinal D2 receptor or blocking the supraspinal D2 receptor produces antinociception. D1 receptor might be not directly involved in the antinociception. l-THP (as a D2 antagonist) as well as spiperone produces antinociception via blocking the supraspinal D2 receptor.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21531416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of pentoxifylline and protein kinase C inhibitor on phorbol ester-induced intercellular adhesion molecule-1 expression in brain microvascular endothelial cells. 己酮茶碱和蛋白激酶C抑制剂对酚酯诱导的脑微血管内皮细胞间粘附分子-1表达的影响。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

Z Y Chu, Y C Rui

引用次数: 0

Schizontocidal effects of oral artesunate on Plasmodium berghei in mice and P knowlesi in monkeys. 口服青蒿琥酯对小鼠伯氏疟原虫和猴子诺氏疟原虫的杀裂作用。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

Y L Shi, G F Li, J H Zhao, J D Yang, D B Ding

引用次数: 0

dl-3-n-butylphthalide attenuates reperfusion-induced blood-brain barrier damage after focal cerebral ischemia in rats. dl-3-正丁苯酞减轻大鼠局灶性脑缺血后再灌注引起的血脑屏障损伤。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

Z Z Chong, Y P Feng

{"title":"dl-3-n-butylphthalide attenuates reperfusion-induced blood-brain barrier damage after focal cerebral ischemia in rats.","authors":"Z Z Chong, Y P Feng","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the protective effect of dl-3-n-butylphthalide (NBP) on blood-brain barrier (BBB) damage induced by reperfusion following focal cerebral ischemia.Methods: Focal cerebral ischemia in rats was performed by inserting a nylon suture into intracranial segment of internal carotid artery to block the origin of middle cerebral artery and reperfusion by withdrawing the nylon suture. Permeability of BBB was determined by extravasation of the protein-bound Evans blue dye to cerebral cortex and further evaluated by immunohistochemical or electronmicroscopic method.Results: Reperfusion for 3 h following focal cerebral ischemia for 3 h produced BBB damage which exhibited the increase in extravasation in cerebral cortex, elevation of the expression of immunoglobulin (IgG), and pore formation in endothelial cell membrane of capillary in cerebral cortex. NBP (5-20 mg.kg-1) decreased the extravasation in a dose-dependent manner. The expression of IgG in cerebral cortex was decreased and the ultrastructure damage of capillaries was alleviated after treatment with NBP. NBP 20 mg.kg-1 also alleviated brain edema caused by 3-h reperfusion following 3-h middle cerebral artery occlusion (MCAO).Conclusion: NBP has protective effect on BBB damage induced by reperfusion after MCAO.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21532055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of berbamine on ATP-induced [Ca2+]i mobilization in cultured vascular smooth muscle cells and cardiomyocytes. 小檗碱对atp诱导的血管平滑肌细胞和心肌细胞[Ca2+]i动员的影响。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

B Y Li, G F Qiao, Y L Zhao, H Zhou, W H Li

{"title":"Effects of berbamine on ATP-induced [Ca2+]i mobilization in cultured vascular smooth muscle cells and cardiomyocytes.","authors":"B Y Li, G F Qiao, Y L Zhao, H Zhou, W H Li","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the effects of berbamine (Ber) on [Ca2+]i homeostasis induced by adenosine triphosphate (ATP) in vascular smooth muscle cells (VSMC) of rabbits and cardiomyocytes of rats.Methods: Both cell types were cultured and loaded with Fura 3-AM. [Ca2+]i was measured by fluorescent intensity (FI) in each cell with confocal microscopy.Results: (1) ATP 30 mumol.L-1 elevated [Ca2+]i in VSMC and cardiomyocytes, FI values reached 660 +/- 258 and 1058 +/- 252 from 250 +/- 84 and 218 +/- 76 at 19 s +/- 5 s and 11.8 s +/- 2.4 s, but FI in nucleus was not changed in VSMC. (2) Ber 30 mumol.L-1 did not affect the resting FI in both cell types, but prolonged the time to peak (P < 0.01) and reduced the FI elevated by ATP (P < 0.01), but not completely inhibited even at 100 mumol.L-1. (3) In D-Hanks' solution or in the presence of egtazic acid (EGTA) 3 mmol.L-1, the inhibitory effect of Ber was not seen (P > 0.05). (4) All effects of Ber on ATP-induced [Ca2+]i mobilization were similar to those of Ver 10 mumol.L-1.Conclusion: In VSMC and cardiomyocytes, ATP-induced CA2+ influx was inhibited by Ber and Ver, while the Ca2+ release was not.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21532057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral effects of rhIFN-alpha 1 against seven influenza viruses. rhIFN-α1 对七种流感病毒的抗病毒作用。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

F Li, Q J Wang, B L Zhu, M Wang

{"title":"Antiviral effects of rhIFN-alpha 1 against seven influenza viruses.","authors":"F Li, Q J Wang, B L Zhu, M Wang","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the antiviral effects of rhIFN-alpha 1 (Chinese silkworm gene recombinant interferon alpha 1) on 7 influenza viruses in MDCK cells and in mouse pneumonia caused by PR8 virus.Methods: 100TCID50 virus (H1N1, H2N2, H3N3, type B, type C, clinical A1, and clinical B) were inoculated into MDCK cells, PR8 viruses were dropped nasally in mice, the antiviral effects of rhIFN-alpha 1 were observed.Results: The minimal effective concentrations of rhIFN-alpha 1 against these 7 influenza viruses were 12.5, 25, 50, 25, 12.5, 25, and 12.5 kU.L-1, respectively. The infectious therapeutic indices of rhIFN-alpha 1 to these viruses in MDCK cells were 8 x 10(3), 4 x 10(3), 2 x 10(3), 4 x 10(3), 8 x 10(3), 4 x 10(3), and 8 x 10(3), respectively. The inhibitory indices of rhIFN-alpha 1 to the 7 influenza viruses in MDCK cells were 3.6, 4.7, 3.5, 3.3, 3.9, 4.6, and 3.5, respectively. The rhIFN-alpha 1 inhibited the intracellular replication of influenza viruses effectively, but did not kill viruses directly. The rhIFN-alpha 1 prolonged the life span of mice infected with pneumonia by influenza virus A strain PR8 to 94.2%-132.7%. It inhibited the inflammation and hyperplasia of interstitial fibers, and decreased the virus titer. The inhibitory rates of rhIFN-alpha 1 to pulmonary-indice were 14.8%-37.4%.Conclusion: rhIFN-alpha 1 inhibited the proliferation of influenza virus and improved the symptom of mouse pneumonia caused by influenza virus.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21532058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subtypes of central nicotinic receptors involved in learning and memory. 参与学习和记忆的中枢烟碱受体亚型。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

L J Chen, R Z Chen

引用次数: 0

Effect of recombinant human basic fibroblast growth factor on stomach ulcers in rats and mice. 重组人碱性成纤维细胞生长因子对大鼠和小鼠胃溃疡的影响。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

J Z Wang, Y J Wu, C M Rao, M T Gao, W G Li

{"title":"Effect of recombinant human basic fibroblast growth factor on stomach ulcers in rats and mice.","authors":"J Z Wang, Y J Wu, C M Rao, M T Gao, W G Li","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the curative effects of recombinant human basic fibroblast growth factor (rh-bFGF) on gastric ulcer healing.Methods: Pylorus ligated, water immersion stress-induced, reserpine-induced, and acetic acid-induced gastric ulcers in rats or mice were prepared. Morphometric analyses on ulcer were performed using microscope and true color image analysis system. The DNA and RNA contents were measured by diphenylamine method and orcinol method, respectively.Results: In acetic acid-induced gastric ulcers in rats, rh-bFGF 2.5-40 kU.kg-1 i.g. accelerated the chronic ulcer healing with a bell-shaped dose-effect curve and the best dosage was 10 kU.kg-1. The regenerated gland epithelium width, density of capillaries in granulation tissue, and collagen content in scar tissues obviously increased in rh-bFGF-treated groups. Simultaneously, rh-bFGF promoted the differentiation and maturation of regenerated glands around ulcers. rh-bFGF 2-4 kU.kg-1 s.c. also increased the synthesis of RNA in ulcer tissues. In acute gastric ulcers, rh-bFGF i.g. was only effective on pylorus ligated ulcers, but showed no effect on total acid output and pepsin activity in gastric juice of rats.Conclusion: rh-bFGF promoted the gastric ulcer healing and improved the quality of gastric ulcer healing.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21531908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-pyroglutamic acid protects rat cortical neurons against sodium glutamate-induced injury. l -焦谷氨酸保护大鼠皮层神经元免受谷氨酸钠诱导的损伤。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

X Q Xiao, G Q Liu

引用次数: 0