Effects of pentoxifylline and protein kinase C inhibitor on phorbol ester-induced intercellular adhesion molecule-1 expression in brain microvascular endothelial cells.
{"title":"Effects of pentoxifylline and protein kinase C inhibitor on phorbol ester-induced intercellular adhesion molecule-1 expression in brain microvascular endothelial cells.","authors":"Z Y Chu, Y C Rui","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>To study the potential roles of protein kinase C (PKC) on expression of intercellular adhesion molecule-1 (ICAM-1) in rat brain microvascular endothelial cells (RBMEC).</p><p><strong>Methods: </strong>ICAM-1 expression in RBMEC was measured by ELISA analyses.</p><p><strong>Results: </strong>Phorbol ester (PMA) enhanced the expression of ICAM-1 in a concentration (10-100 nmol.L-1) and time (4-16 h)-dependent manner in RBMEC. Pentoxifylline (PTX) 1-100 mumol.L-1 and the PKC inhibitor H7 5-50 mumol.L-1 prevented PMA-induced stimulation of ICAM-1 expression. At PTX 100 mumol.L-1 and H7 50 mumol.L-1, they reached maximal inhibitory effects [ICAM-1 expression (A) from (0.410 +/- 0.014) to (0.175 +/- 0.022) and (0.182 +/- 0.013), respectively; P < 0.01].</p><p><strong>Conclusion: </strong>Activation of PKC in RBMEC is associated with increased expression of ICAM-1 in RBMEC. PTX and H7 preincubation may inhibit PKC-induced up-regulation of ICAM-1.</p>","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Aim: To study the potential roles of protein kinase C (PKC) on expression of intercellular adhesion molecule-1 (ICAM-1) in rat brain microvascular endothelial cells (RBMEC).
Methods: ICAM-1 expression in RBMEC was measured by ELISA analyses.
Results: Phorbol ester (PMA) enhanced the expression of ICAM-1 in a concentration (10-100 nmol.L-1) and time (4-16 h)-dependent manner in RBMEC. Pentoxifylline (PTX) 1-100 mumol.L-1 and the PKC inhibitor H7 5-50 mumol.L-1 prevented PMA-induced stimulation of ICAM-1 expression. At PTX 100 mumol.L-1 and H7 50 mumol.L-1, they reached maximal inhibitory effects [ICAM-1 expression (A) from (0.410 +/- 0.014) to (0.175 +/- 0.022) and (0.182 +/- 0.013), respectively; P < 0.01].
Conclusion: Activation of PKC in RBMEC is associated with increased expression of ICAM-1 in RBMEC. PTX and H7 preincubation may inhibit PKC-induced up-regulation of ICAM-1.
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