细胞因子促进人BT325星形细胞瘤细胞产生一氧化氮。

H W Zhao, X Y Li
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引用次数: 0

摘要

目的:研究脂多糖(LPS)和炎症前细胞因子对培养星形胶质细胞一氧化氮(NO)生成的影响。方法:采用Griess法测定上清液中亚硝酸盐含量。结果:培养的人BT325星形细胞瘤细胞在培养4 h时开始产生NO,在12 h时达到峰值(15.0 mummol . l -1),在72 h内保持较高水平(15.0 ~ 17.5 mummol . l -1), LPS 1 mg可增强NO的产生。L-1,干扰素γ (ifn - γ) 100 kU。L-1,肿瘤坏死因子- α (tnf - α) 100 kU。L-1,白细胞介素(IL)-1 100 kU。L-1,或il - 2100kul -1。tnf - α、IL-1、IL-2或以上四种细胞因子的混合物的增强程度更高。结论:兴奋剂和炎症前细胞因子可促进星形胶质细胞生成NO。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytokines enhance nitric oxide production from human BT325 astrocytoma cells.

Aim: To study the effects of lipopolysaccharides (LPS) and pre-inflammatory cytokines on nitric oxide (NO) production from cultured astrocytes.

Methods: Nitrites in supernatants were measured with Griess assay.

Results: The NO production from cultured human BT325 astrocytoma cells started when cultured for 4 h, reached the peak concentration (15.0 mumol.L-1) at 12 h, maintained a high level (15.0-17.5 mumol.L-1) up to 72 h, and was enhanced by LPS 1 mg.L-1, interferon-gamma (IFN-gamma) 100 kU.L-1, tumor necrosis factor-alpha (TNF-alpha) 100 kU.L-1, interleukin (IL)-1 100 kU.L-1, or IL-2 100 kU.L-1. The enhancements of TNF-alpha, IL-1, IL-2, or the mixture of the above four cytokines were higher.

Conclusion: Stimulants and pre-inflammatory cytokines enhance astrocytes producing NO.

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