Effects of berbamine on ATP-induced [Ca2+]i mobilization in cultured vascular smooth muscle cells and cardiomyocytes.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

B Y Li, G F Qiao, Y L Zhao, H Zhou, W H Li

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Abstract

Aim: To study the effects of berbamine (Ber) on [Ca2+]i homeostasis induced by adenosine triphosphate (ATP) in vascular smooth muscle cells (VSMC) of rabbits and cardiomyocytes of rats.

Methods: Both cell types were cultured and loaded with Fura 3-AM. [Ca2+]i was measured by fluorescent intensity (FI) in each cell with confocal microscopy.

Results: (1) ATP 30 mumol.L-1 elevated [Ca2+]i in VSMC and cardiomyocytes, FI values reached 660 +/- 258 and 1058 +/- 252 from 250 +/- 84 and 218 +/- 76 at 19 s +/- 5 s and 11.8 s +/- 2.4 s, but FI in nucleus was not changed in VSMC. (2) Ber 30 mumol.L-1 did not affect the resting FI in both cell types, but prolonged the time to peak (P < 0.01) and reduced the FI elevated by ATP (P < 0.01), but not completely inhibited even at 100 mumol.L-1. (3) In D-Hanks' solution or in the presence of egtazic acid (EGTA) 3 mmol.L-1, the inhibitory effect of Ber was not seen (P > 0.05). (4) All effects of Ber on ATP-induced [Ca2+]i mobilization were similar to those of Ver 10 mumol.L-1.

Conclusion: In VSMC and cardiomyocytes, ATP-induced CA2+ influx was inhibited by Ber and Ver, while the Ca2+ release was not.

本刊更多论文

小檗碱对atp诱导的血管平滑肌细胞和心肌细胞[Ca2+]i动员的影响。

目的:研究小檗碱(Ber)对三磷酸腺苷(ATP)诱导的兔血管平滑肌细胞(VSMC)和大鼠心肌细胞[Ca2+]i稳态的影响。方法:两种细胞均培养并加载Fura 3-AM。用共聚焦显微镜测定每个细胞中的[Ca2+]i荧光强度(FI)。结果:(1)ATP 30mumol。L-1升高VSMC和心肌细胞的[Ca2+]i，在19 s +/- 5 s和11.8 s +/- 2.4 s时，FI值从250 +/- 84和218 +/- 76分别达到660 +/- 258和1058 +/- 252，但VSMC细胞核内的FI没有变化。(2)她30岁。L-1对两种细胞的静息FI均无影响，但延长了达到峰值的时间(P < 0.01)，降低了ATP升高的FI (P < 0.01)，即使在100 mmol .L-1时也未完全抑制。(3)在D-Hanks溶液中或在EGTA (EGTA)存在下3 mmol。L-1时，未见Ber的抑制作用(P > 0.05)。(4) Ber对atp诱导的[Ca2+]i动员的所有影响与Ver 10 mumol.L-1相似。结论:在VSMC和心肌细胞中，Ber和Ver抑制atp诱导的CA2+内流，而CA2+释放不受抑制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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