Antiviral effects of rhIFN-alpha 1 against seven influenza viruses.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-08-01

F Li, Q J Wang, B L Zhu, M Wang

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引用次数: 0

Abstract

Aim: To study the antiviral effects of rhIFN-alpha 1 (Chinese silkworm gene recombinant interferon alpha 1) on 7 influenza viruses in MDCK cells and in mouse pneumonia caused by PR8 virus.

Methods: 100TCID50 virus (H1N1, H2N2, H3N3, type B, type C, clinical A1, and clinical B) were inoculated into MDCK cells, PR8 viruses were dropped nasally in mice, the antiviral effects of rhIFN-alpha 1 were observed.

Results: The minimal effective concentrations of rhIFN-alpha 1 against these 7 influenza viruses were 12.5, 25, 50, 25, 12.5, 25, and 12.5 kU.L-1, respectively. The infectious therapeutic indices of rhIFN-alpha 1 to these viruses in MDCK cells were 8 x 10(3), 4 x 10(3), 2 x 10(3), 4 x 10(3), 8 x 10(3), 4 x 10(3), and 8 x 10(3), respectively. The inhibitory indices of rhIFN-alpha 1 to the 7 influenza viruses in MDCK cells were 3.6, 4.7, 3.5, 3.3, 3.9, 4.6, and 3.5, respectively. The rhIFN-alpha 1 inhibited the intracellular replication of influenza viruses effectively, but did not kill viruses directly. The rhIFN-alpha 1 prolonged the life span of mice infected with pneumonia by influenza virus A strain PR8 to 94.2%-132.7%. It inhibited the inflammation and hyperplasia of interstitial fibers, and decreased the virus titer. The inhibitory rates of rhIFN-alpha 1 to pulmonary-indice were 14.8%-37.4%.

Conclusion: rhIFN-alpha 1 inhibited the proliferation of influenza virus and improved the symptom of mouse pneumonia caused by influenza virus.

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rhIFN-α1 对七种流感病毒的抗病毒作用。

目的：研究 rhIFN-α1（家蚕基因重组干扰素α1）对 MDCK 细胞中 7 种流感病毒和 PR8 病毒引起的小鼠肺炎的抗病毒作用。方法：将100TCID50病毒（H1N1、H2N2、H3N3、B型、C型、临床A1和临床B）接种到MDCK细胞中，将PR8病毒滴鼻到小鼠体内，观察rhIFN-α1的抗病毒作用：结果：rhIFN-α1对这7种流感病毒的最小有效浓度分别为12.5、25、50、25、12.5、25和12.5 kU.L-1。rhIFN-α 1 在 MDCK 细胞中对这些病毒的感染治疗指数分别为 8 x 10(3)、4 x 10(3)、2 x 10(3)、4 x 10(3)、8 x 10(3)、4 x 10(3)和 8 x 10(3)。rhIFN-α 1 对 MDCK 细胞中 7 种流感病毒的抑制指数分别为 3.6、4.7、3.5、3.3、3.9、4.6 和 3.5。rhIFN-α 1 能有效抑制流感病毒在细胞内的复制，但不能直接杀死病毒。rhIFN-α 1 可使感染甲型流感病毒 PR8 株肺炎的小鼠寿命延长 94.2%-132.7%。它抑制了炎症和间质纤维的增生，降低了病毒滴度。结论：rhIFN-α1能抑制流感病毒的增殖，改善流感病毒引起的小鼠肺炎的症状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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