Intraventricular vascular endothelial growth factor antibody increases infarct volume following transient cerebral ischemia.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

W L Bao, S D Lu, H Wang, F Y Sun

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Abstract

Aim: To clarify the role of vascular endothelial growth factor (VEGF) in neuronal damage induced by cerebral ischemia.

Methods: Expression of VEGF in adult rat brain was measured by immunohistochemistry. Transient middle cerebral artery occlusion (MCAO) model was induced by placing a nylon thread in the lumen of the internal carotid artery. The infarct volume was shown with 2,3,5-triphenyltetrazolium chloride (TTC) staining and quantitated by computer image analyzer with and without VEGF antibody treatment.

Results: VEGF expression was widely distributed in neuronal cells besides vascular endothelial cells, and the neuronal distribution of VEGF was specific. After intraventricular treatment with VEGF antibody (0.1 g.L-1 daily, for 7 d following the ischemia), infarct volume in the antibody treatment was increased versus vehicle-treated rats [(21.6 +/- 2.7 vs 16 +/- 6) mm3, P < 0.05] respectively.

Conclusion: Intraventricular injection of VEGF antibody increased the infarct volume after focal cerebral ischemia in rats, suggesting that expression of neuronal VEGF may be one of neuronal protective mechanisms.

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脑室内血管内皮生长因子抗体增加短暂性脑缺血后的梗死体积。

目的:探讨血管内皮生长因子(VEGF)在脑缺血神经元损伤中的作用。方法:采用免疫组化方法检测成年大鼠脑组织中VEGF的表达。在颈内动脉腔内置入尼龙线，建立短暂性大脑中动脉闭塞(MCAO)模型。用2,3,5-三苯基四氯化氮(TTC)染色显示梗死面积，用计算机图像分析仪定量测定VEGF抗体处理和未处理的梗死面积。结果:除血管内皮细胞外，VEGF在神经细胞中广泛表达，且在神经细胞中的分布具有特异性。经血管内皮生长因子抗体(0.1 g。在缺血后的7 d内，抗体组的梗死面积比对照组大[(21.6 +/- 2.7 vs 16 +/- 6) mm3, P < 0.05]。结论:脑室内注射VEGF抗体可增加局灶性脑缺血大鼠的梗死面积，提示神经元VEGF的表达可能是神经元的保护机制之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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