Intraventricular vascular endothelial growth factor antibody increases infarct volume following transient cerebral ischemia.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

W L Bao, S D Lu, H Wang, F Y Sun

{"title":"Intraventricular vascular endothelial growth factor antibody increases infarct volume following transient cerebral ischemia.","authors":"W L Bao, S D Lu, H Wang, F Y Sun","doi":"","DOIUrl":null,"url":null,"abstract":"Aim: To clarify the role of vascular endothelial growth factor (VEGF) in neuronal damage induced by cerebral ischemia.Methods: Expression of VEGF in adult rat brain was measured by immunohistochemistry. Transient middle cerebral artery occlusion (MCAO) model was induced by placing a nylon thread in the lumen of the internal carotid artery. The infarct volume was shown with 2,3,5-triphenyltetrazolium chloride (TTC) staining and quantitated by computer image analyzer with and without VEGF antibody treatment.Results: VEGF expression was widely distributed in neuronal cells besides vascular endothelial cells, and the neuronal distribution of VEGF was specific. After intraventricular treatment with VEGF antibody (0.1 g.L-1 daily, for 7 d following the ischemia), infarct volume in the antibody treatment was increased versus vehicle-treated rats [(21.6 +/- 2.7 vs 16 +/- 6) mm3, P < 0.05] respectively.Conclusion: Intraventricular injection of VEGF antibody increased the infarct volume after focal cerebral ischemia in rats, suggesting that expression of neuronal VEGF may be one of neuronal protective mechanisms.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: To clarify the role of vascular endothelial growth factor (VEGF) in neuronal damage induced by cerebral ischemia.

Methods: Expression of VEGF in adult rat brain was measured by immunohistochemistry. Transient middle cerebral artery occlusion (MCAO) model was induced by placing a nylon thread in the lumen of the internal carotid artery. The infarct volume was shown with 2,3,5-triphenyltetrazolium chloride (TTC) staining and quantitated by computer image analyzer with and without VEGF antibody treatment.

Results: VEGF expression was widely distributed in neuronal cells besides vascular endothelial cells, and the neuronal distribution of VEGF was specific. After intraventricular treatment with VEGF antibody (0.1 g.L-1 daily, for 7 d following the ischemia), infarct volume in the antibody treatment was increased versus vehicle-treated rats [(21.6 +/- 2.7 vs 16 +/- 6) mm3, P < 0.05] respectively.

Conclusion: Intraventricular injection of VEGF antibody increased the infarct volume after focal cerebral ischemia in rats, suggesting that expression of neuronal VEGF may be one of neuronal protective mechanisms.

本刊更多论文

脑室内血管内皮生长因子抗体增加短暂性脑缺血后的梗死体积。

目的:探讨血管内皮生长因子(VEGF)在脑缺血神经元损伤中的作用。方法:采用免疫组化方法检测成年大鼠脑组织中VEGF的表达。在颈内动脉腔内置入尼龙线，建立短暂性大脑中动脉闭塞(MCAO)模型。用2,3,5-三苯基四氯化氮(TTC)染色显示梗死面积，用计算机图像分析仪定量测定VEGF抗体处理和未处理的梗死面积。结果:除血管内皮细胞外，VEGF在神经细胞中广泛表达，且在神经细胞中的分布具有特异性。经血管内皮生长因子抗体(0.1 g。在缺血后的7 d内，抗体组的梗死面积比对照组大[(21.6 +/- 2.7 vs 16 +/- 6) mm3, P < 0.05]。结论:脑室内注射VEGF抗体可增加局灶性脑缺血大鼠的梗死面积，提示神经元VEGF的表达可能是神经元的保护机制之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Zhongguo yao li xue bao = Acta pharmacologica Sinica

自引率

0.00%

发文量