{"title":"Inhibition by baclofen of NMDA-activated current in rat dorsal root ganglion neurons.","authors":"J Q Si, Z W Li","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>To explore the modulatory effect of baclofen on NMDA-activated current in rat dorsal root ganglion (DRG) neurons.</p><p><strong>Methods: </strong>Whole-cell patch-clamp technique was used to record NMDA-activated current in isolated DRG neurons. Drugs were applied by rapid solution exchange.</p><p><strong>Results: </strong>Preapplication of baclofen 1-100 mumol.L-1 induced a concentration-dependent inhibition of the inward NMDA-activated current markedly. NMDA (100 mumol.L-1)-activated current was inhibited by 52% +/- 14% (n = 11, P < 0.01) by preapplication of baclofen 100 mumol.L-1. The inhibitory effect of baclofen was reversible, and was removed by saclofen 100 mumol.L-1, which was a selective antagonist of GABAB receptor.</p><p><strong>Conclusion: </strong>Preapplication of baclofen exerts an inhibitory effect on NMDA-activated current in the primary sensory neurons.</p>","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Aim: To explore the modulatory effect of baclofen on NMDA-activated current in rat dorsal root ganglion (DRG) neurons.
Methods: Whole-cell patch-clamp technique was used to record NMDA-activated current in isolated DRG neurons. Drugs were applied by rapid solution exchange.
Results: Preapplication of baclofen 1-100 mumol.L-1 induced a concentration-dependent inhibition of the inward NMDA-activated current markedly. NMDA (100 mumol.L-1)-activated current was inhibited by 52% +/- 14% (n = 11, P < 0.01) by preapplication of baclofen 100 mumol.L-1. The inhibitory effect of baclofen was reversible, and was removed by saclofen 100 mumol.L-1, which was a selective antagonist of GABAB receptor.
Conclusion: Preapplication of baclofen exerts an inhibitory effect on NMDA-activated current in the primary sensory neurons.
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