Basic fibroblast growth factor enhanced LAK cell cytotoxicities against human bladder neoplasm cells.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-03-01

Z P Wang, G L Duan, Y R Chen, D S Qin, G D Liu, H G Niu

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Abstract

Aim: To study the effects of basic fibroblast growth factor (bFGF) on the proliferation of lymphokine-activated killer (LAK) cells from patients with bladder cancer and LAK cells cytolysis against bladder tumor cells.

Methods: LAK cell proliferation was assayed in the presence of various concentrations of bFGF combined with interleukin-2 (IL-2) by cell count. Cytotoxicity of LAK cells against bladder cancer cell line EJ cells and bladder tumor cells (BTC) from patients was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays.

Results: The proliferation of peripheral blood monocytes (PBMC) was inhibited by bFGF 5 micrograms.L-1. bFGF did not affect the stimulation of LAK cells induced by IL-2. The LAK cell numbers in the combination of IL-2 with bFGF were not significantly different compared with that treated with IL-2 alone. bFGF enhanced cytotoxicity of LAK cells against bladder cancer cell line EJ cells or BTC, respectively.

Conclusion: Although the proliferation of PBMC was inhibited by bFGF, bFGF increased LAK cell cytotoxicity against bladder neoplasm cells.

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碱性成纤维细胞生长因子增强LAK细胞对人膀胱肿瘤细胞的细胞毒性。

目的:研究碱性成纤维细胞生长因子(bFGF)对膀胱癌患者淋巴因子活化杀伤细胞(LAK)增殖的影响及LAK细胞对膀胱癌细胞的细胞溶解作用。方法:采用细胞计数法测定不同浓度bFGF联合白细胞介素-2 (IL-2)作用下LAK细胞的增殖情况。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)法测定LAK细胞对膀胱癌细胞系EJ细胞和膀胱癌细胞(BTC)的细胞毒性。结果:bFGF 5 μ g . l -1对外周血单核细胞(PBMC)增殖有抑制作用。bFGF不影响IL-2对LAK细胞的刺激作用。白细胞介素-2与bFGF联合治疗的LAK细胞数量与单独治疗的LAK细胞数量无显著差异。bFGF分别增强LAK细胞对膀胱癌细胞系EJ细胞和BTC的细胞毒性。结论:bFGF虽能抑制PBMC的增殖，但能增强LAK细胞对膀胱肿瘤细胞的细胞毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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