中华口腔医学杂志最新文献

{"title":"[Recent evaluation and analysis of the clinical effect of two closed treatment methods in children with intracapsular condyle fracture].","authors":"H Wang, Y Z Wang, J G An","doi":"10.3760/cma.j.cn112144-20240105-00013","DOIUrl":"10.3760/cma.j.cn112144-20240105-00013","url":null,"abstract":"<p><p><b>Objective:</b> To summarize and analyze clinical characteristics of pediatric mandibular condylar fractures and the long-term therapeutic effects of closed treatment. <b>Methods:</b> A retrospective study was conducted for pediatric condylar fracture in the Department of Oral and Maxillofacial Surgery of Peking University School and Hospital of Stomatology from October 2015 to October 2019, including 33 males (67.3%) and 16 females (32.7%), with an average age of (8.3±2.1) years old. According to the treatment methods, the children were divided into two groups: group A was a removable occlusal splint accompanied with functional exercise, group B was a pure functional exercise. Forty-nine cases (76 sides) children with intracapsular condylar fracture were included in this study. Twenty-three cases in group A and 26 cases in group B. The maximum month opening increased from (20.0±6.2) mm to (46.0±5.3) mm 6 months after closed treatment. Subjective evaluation, special examination, qualitative analysis and quantitative analysis of imaging were used to evaluate the condylar remodeling and functional recovery of temporomandibular joint in two groups of children after closed treatment of intracapsular condyle fracture. <b>Results:</b> There was no significant difference in subjective evaluation, maximum opening examination, mouth open-type, mandibular protrusion, lateral movement and qualitative analysis of imaging at the six-month follow-up after injury. Quantitative imaging measurements showed that the condylar anteroposterior diameter and condylar height in Group B were significantly higher than those in Group A after 1 year of injury. <b>Conclusions:</b> Closed treatment for pediatric condylar fractures can achieve satisfactory results. After 6 months of injury, the children in the two groups could recover the temporomandibular joint function and promote the condylar adaptative remodeling.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 10","pages":"1008-1013"},"PeriodicalIF":0.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Three-dimensional analysis of force distribution on maxillary dentition while distalization of first and second molars simultaneously with clear aligners].","authors":"X H He, Z X Yang, X Y Zhang, Y J Fan, Y R Hu, Z X Gu","doi":"10.3760/cma.j.cn112144-20240109-00016","DOIUrl":"10.3760/cma.j.cn112144-20240109-00016","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To explore the force distribution on the maxillary dentition when the first and second molars distalized simultaneously with different step sizes using clear aligners &lt;i&gt;in vitro&lt;/i&gt; in order to provide a theoretical basis for the rational design of molar distalization. &lt;b&gt;Methods:&lt;/b&gt; Clear aligners were designed to simultaneously distalize the maxillary first and second molars bilaterally, with rectangular attachments placed on the buccal surfaces of the first and second premolars, as well as the second molars. Based on different step sizes, the aligners were divided into three groups: Group A (0.15 mm per step), Group B (0.20 mm per step), and Group C (0.25 mm per step). Ten aligners were fabricated for each group using 0.76 mm thick polyethylene terephthalate glycol (PET-G) sheets. A three-dimensional force measurement system was used to measure the forces exerted on each tooth by the aligners, the first and second molars served as the target teeth and the remaining teeth as anchorage teeth. The three-dimensional force data were compared among the three groups. &lt;b&gt;Results:&lt;/b&gt; In the mesiodistal direction, the forces on the central and lateral incisors were relatively small among all three groups, with no statistically significant differences (&lt;i&gt;P&gt;&lt;/i&gt;0.05). However, significant differences were observed in the forces on the canines, first premolars, second premolars, first molars, and second molars (&lt;i&gt;P&lt;&lt;/i&gt;0.05). The distal forces on the second molars in Groups B and C were (6.13±1.45) N and (6.83±1.58) N, respectively, significantly higher than that in Group A [(3.51±1.01) N] (&lt;i&gt;P&lt;&lt;/i&gt;0.05). The distal force on the first molars in Group C [(6.62±0.89) N] was significantly higher than that in Groups A and B (&lt;i&gt;P&lt;&lt;/i&gt;0.05). The mesial reactive forces on the first and second premolars in Groups B and C were significantly higher than those in Group A (&lt;i&gt;P&lt;&lt;/i&gt;0.05). The mesial reactive force on the canines in Group C [(-2.98±1.33) N] was significantly higher than that in Group A [(-1.69±0.68) N] (&lt;i&gt;P&lt;&lt;/i&gt;0.05), while there were no significant differences between Groups B and C in the forces on the canines, first premolars, and second premolars (&lt;i&gt;P&gt;&lt;/i&gt;0.05). In the buccolingual direction, there were no statistically significant differences in the forces on the central and lateral incisors among three groups (&lt;i&gt;P&gt;&lt;/i&gt;0.05), but significant differences were observed in the forces on the canines, second premolars, and second molars (&lt;i&gt;P&lt;&lt;/i&gt;0.05). The buccolingual forces on the canines, second premolars, and second molars in Group B were (-0.56±0.54), (-2.07±0.95), (1.13±0.55) N, respectively, significantly higher than those in Group A (&lt;i&gt;P&lt;&lt;/i&gt;0.05), but there were no significant differences compared to Group C (&lt;i&gt;P&gt;&lt;/i&gt;0.05). Compared to the mesiodistal and buccolingual forces, the vertical forces on the target and anchorage teeth were relatively small in all three groups. &lt;b&gt;Conclusions:&lt;/b&gt; When using 0.76 ","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 10","pages":"1037-1043"},"PeriodicalIF":0.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Retrospective analysis of 62 cases who received clinical application of artificial temporomandibular joint].","authors":"P Wang, Y Liu, N Jiang, R Y Bi, P Y Cao, W L Zhao, M J Ren, S S Zhu","doi":"10.3760/cma.j.cn112144-20240611-00239","DOIUrl":"10.3760/cma.j.cn112144-20240611-00239","url":null,"abstract":"<p><p><b>Objective:</b> To conduct a retrospective study on the treatment outcomes of patients who underwent artificial temporomandibular joint (TMJ) replacement surgery and to evaluate the effectiveness of artificial TMJ treatment. <b>Methods:</b> This study selected 62 patients who received standard Biomet artificial TMJ treatment at Department of Orthognathic and TMJ Surgery, West China Hospital of Stomatology, Sichuan University from May 2010 to September 2023 as the study subjects. Among them, there were 15 male patients and 47 female patients. The average age was 33.5 years old(ranging from 18 to 67 years). This study statistically analyzed postoperative indicators, including maximum mouth opening, forward jaw movement, lateral movement, postoperative pain scores, and patient satisfaction. <b>Results:</b> This study included a total of 62 patients with 99 TMJ joints. No infections occurred postoperatively. The average follow-up period was 33.7 months (ranging from 7 to 170 months). At 6 months postoperatively, the mean mouth opening was (36.1±6.2) mm, lateral movement was (2.1±0.9) mm, and forward jaw movement was (1.0±0.9) mm. The pain visual analog scale score at 6 months postoperatively was (2.8±0.6), and patient satisfaction with the surgery was (8.8±1.1). Spiral CT scans conducted after surgery showed no joint dislocation or migration, and the artificial joint remained stable during the follow-up period. <b>Conclusions:</b> Artificial TMJ replacement is a valuable method for effectively restoring TMJ structure and essential functions related to mouth opening and chewing. It is worthy of promotion as a reconstructive approach for the temporomandibular joint.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 10","pages":"1014-1018"},"PeriodicalIF":0.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Introduction and interpretation of The European Society of Endodontology S3-level clinical practice guideline for treatment of pulpal and apical disease].","authors":"X Y Huang, C Lin, Z Chen, Y X Lin, L Zhang, Z Chen","doi":"10.3760/cma.j.cn112144-20240404-00134","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20240404-00134","url":null,"abstract":"<p><p>The European Society of Endodontology published the S3-level clinical practice guideline for the treatment of pulpal and apical disease in October 2023, which provides best current therapeutic strategies supported by scientific evidences. The guideline was divided into four parts: the diagnosis and treatment of pulpitis, diagnosis and nonsurgical treatment of apical periodontitis, surgical treatment of apical periodontitis, and regenerative treatment. This article aims to introduce and interpret the guideline, and to better manage patients with pulpitis and apical periodontitis for preserving teeth over a patient's lifetime in China.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 9","pages":"871-878"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of clear aligners combined with maxillary protractor on mixed dentition patient with skeletal class Ⅲ malocclusion: a finite element study].","authors":"Y N Ma, R Y Qiang, Z L Jin","doi":"10.3760/cma.j.cn112144-20240516-00207","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20240516-00207","url":null,"abstract":"<p><p><b>Objective:</b> To compare the effects of expansion screw with maxillary protractor and clear aligners combined with maxillary protractor on the maxilla and maxillary dentition of mixed dentition patients with class Ⅲ skeletal malocclusion using finite element analysis, further providing clinical guidance for clear aligner treatment. <b>Methods:</b> A finite element model was established based on maxillofacial cone-beam CT data of a 10-year-old boy with mixed dentition, class Ⅲ skeletal malocclusion, who visited Department of Orthodontics, School of Stomatology, The Fourth Military Medical University in January, 2024. The expander with protractor group (group A) and the clear aligners with protractor group (group B) were divided. The arch was extended by 0.25 mm in both groups, and the forward pull force was 2.94 N (working condition 1) and 4.90 N (working condition 2), respectively. The initial displacement trend of maxilla and maxillary dentition in two groups under two working conditions were evaluated. <b>Results:</b> Under two working conditions, the maxilla of both groups showed clockwise rotation and labial inclination. The labial inclination of maxilla was more significant in clear aligners with protractor group, about 2.2-3.0 times that of expander with protractor group under the same working condition. Maxillary dentition showed mesial and buccal displacement, with anterior teeth extrusion and posterior teeth intrusion in two groups under two working conditions. Under the working condition 1 and 2, the labial displacement of central incisor of clear aligners with protractor group (-0.065, -0.089 mm) were greater than that in expander with protractor group (-0.024, -0.024 mm). Under two working conditions, the posterior teeth of expander with protractor group moved close to the buccal bodily direction, while those of clear aligners with protractor group moved tilted towards the buccal direction in the horizontal direction. The forward displacement trend of maxilla and maxillary dentition in clear aligners with protractor group was more obvious than expander with protractor group with the increase of the forward pull force. Under two working conditions, the anterior teeth' s hydrostatic stress of periodontal membrane and the equivalent stress of alveolar bone in clear aligners with protractor group were higher than those in expander with protractor group, mainly concentrated on the labial cervical region of the incisor. <b>Conclusions:</b> Clear aligners combined with maxillary protractor can produce forward force on the maxilla, but labial inclination occurs in the anterior teeth. It can be an effective orthopedic treatment strategy for mixed dentition patients with class Ⅲ skeletal malocclusion.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 9","pages":"919-926"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prevalence of dentinal hypersensitivity and related factors in Chinese urban adults].","authors":"Z Y Wang, W S Rong, Y S Zhong, J G Tian, X Chen, M Zhao, L H Cui, M Q Du, J B Li, D Y Hu","doi":"10.3760/cma.j.cn112144-20231229-00312","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20231229-00312","url":null,"abstract":"<p><p><b>Objective:</b> To assess the prevalence of dentinal hypersensitivity (DH) and related factors in urban adults in China. <b>Methods:</b> The study was designed as an observational, cross-sectional epidemiological study carried out in adults aged 18-69 years old in seven cities (Beijing, Shanghai, Wuhan, Chengdu, Xi'an, Guangzhou, and Harbin) of China. The study was conducted from March 2021 to May 2023. Patients were required to complete a questionnaire regarding the subjects' socio-economic factors, dietary behavior, oral health behavior and personal antecedent factors. DH was clinically diagnosed by judging whether the tooth cold air stimulation provoked DH or not, and recorded by investigator pain rating Schiff score. Compare the findings of six cities (Harbin excluded) with a similar study conducted in 2008. <b>Results:</b> In total, 11 622 subjects from seven cities in China participated the study. Fifty two point two percent (6 072/11 622) of subjects reported DH in questionnaire, 36.7% (4 266/11 622) of subjects reported experiencing DH in response to cold air stimulation for at least one study tooth. Risk factors including age, sex, city, toothbrush method and acid reflux showed marked associations with DH (<i>P</i><0.05). The prevalence of DH of urban residents in six cities (Harbin excluded) was 33.7% (3 335/9 882), higher than that in 2008 [29.7%(2 354/7 939)]. <b>Conclusions:</b> Overall, DH was common among urban adults in China and the prevalence increased in recent years. Better understanding of DH and its associated factors should be considered in its prevention and management by dental professionals.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 9","pages":"927-934"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Accuracy of multi-task network based on vision Transformer in the three-dimensional upper airway analysis].","authors":"S H Jin, H J Han, F Chen, X Y Guan, F Hua, H He","doi":"10.3760/cma.j.cn112144-20240514-00205","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20240514-00205","url":null,"abstract":"<p><p><b>Objective:</b> To explore the accuracy of a multi-task model based on vision Transformer for analyzing the three-dimensional (3D) upper airway and its subregions, and to evaluate its clinical applicability. <b>Methods:</b> According to the inclusion and exclusion criteria, cone-beam CT (CBCT) data of 10 patients [4 males and 6 females, (20.8±2.7) years] who had their first visit to the Department of Orthodontics in the Hospital of Stomatology, Wuhan University from January 2012 to January 2020 were retrospectively selected. The 3D slicer software was used to segment the upper airway and pharyngeal airway and measure their volumes as the gold standard. The Dolphin 3D software was used to segment the pharyngeal airway and its subregions and measure their volumes as the gold standard. A multi-task model based on vision Transformer developed by the research team for automatic segmentation and volume measurement of the upper airway and its subregions. All the measurements were conducted by the same attending physician. The Bland-Altman analysis and intraclass correlation coefficient (<i>ICC</i>) were used to evaluate the consistency between the multi-task network and the gold standard in the upper airway segmentation and volume measurements, and the paired <i>t</i> test was used to compare the differences between the multi-tasking model and the gold standard. <b>Results:</b> The mean volume deviation of the upper airway segmented by multi-task model and 3D Slicer was -979.6 mm³, and the <i>ICC</i> was 0.97. The mean volume deviation of the pharyngeal airway, nasopharynx, velopharynx, glossopharynx and hypopharynx segmented by multi-task network and Dolphin 3D were 2 069.5, -950.1, -823.6, -813.9 and 4 003.4 mm³, respectively. In addition, <i>ICC</i> in pharyngeal airway, nasopharynx, velopharynx, glossopharynx and hypopharynx were 0.97, 0.94, 0.96, 0.96 and 0.69, respectively. <b>Conclusions:</b> The multi-task model based on vision Transformer produced different errors in the segmentation of 3D upper airway and its subregions. The segmentation of the nasopharynx, velopharynx and glossopharynx was in good agreement with the gold standard, while the segmentation of hypopharynx was poor, suggesting that the robustness and generalization of this model should be further enhanced.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 9","pages":"911-918"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Early orthodontic treatment of mouth breathing related to malocclusion in children].","authors":"T T Zhao, H He","doi":"10.3760/cma.j.cn112144-20240506-00183","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20240506-00183","url":null,"abstract":"<p><p>Mouth breathing is one of the most common oral and maxillofacial abnormalities in children. Children with mouth breathing are often accompanied by obstructive sleep apnea, which not only affects children's dentofacial development, leading to the occurrence of malocclusion, but also may lead to a series of serious systemic complications. The diagnosis and treatment of mouth breathing and its related malocclusions require multidisciplinary collaboration. This paper discusses the etiology and harm of mouth breathing, diagnosis of mouth breathing related problems, correlation between mouth breathing and malocclusion, treatment of mouth breathing related malocclusions.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 9","pages":"892-897"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Diagnosis and treatment of class Ⅱ malocclusion in adolescents].","authors":"B Fang","doi":"10.3760/cma.j.cn112144-20240527-00220","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20240527-00220","url":null,"abstract":"<p><p>Class Ⅱ malocclusion is one of the factors inducing poor appearance and occlusal dysfunction in adolescents, which affects their physical and mental development. The success of treatment in correction class Ⅱ malocclusion in adolescents is dependent on the appropriate choice of therapy timing and methods, because of multifaceted causes. And puberty represents an essential period for development improvement. In the meantime, precise risk prevention and management are also conducive to the enhance treatment outcomes. A comprehension of the diagnosis, therapy, and risk control of adolescents class Ⅱ malocclusion will be discussed.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 9","pages":"879-885"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment and biological characteristics of a human buccal mucosa squamous cell carcinoma cell line SCC117].","authors":"C Meng, S L Li, Y X Wang, J Fu, X Y Ge","doi":"10.3760/cma.j.cn112144-20240428-00171","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20240428-00171","url":null,"abstract":"<p><p><b>Objective:</b> To establish a human buccal mucosa squamous cell carcinoma (BMSCC) cell line SCC117 in China, analyze and identify its basic biological characteristics. <b>Methods:</b> A 59-year-old Chinese male patient with BMSCC in the Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology in January 2011 was included in this study, his surgical specimens were primary cultured <i>in vitro</i> by improved tissue block culture method. The BMSCC cell line SCC117 was established after continuous passage and stable growth. The morphological characteristics of the cells were observed by light and electron microscope, and their basic biological characteristics were analyzed by growth curve, chromosome karyotype and xenotransplantation tumorigenicity in nude mice experiment. The expressions of cytokeratin (CK14), tumor-related proteins retinoblastoma tumor suppressor protein (RB), P53, E-cadherin, P21, phosphatase and tensin homolog deleted on chromosome ten (PTEN) were detected by immunohistochemical and human papilloma virus (HPV) were tested by PCR. SCC117 was identified by short tandem repeat (STR) analysis of genomic DNA. <b>Results:</b> SCC117, a human BMSCC cell line, had been continuously subcultured <i>in vitro</i> for more than 150 generations. The cells grew in polygonal mosaic and lost contact inhibition, the typical desmosomes and tensional fibrils were observed by electron microscope, and CK14 was positive by immunohistochemistry. The doubling time was 40.16 h, the chromosome mode of the cell line was concentrated between 67 and 69, hypo-triploid. All 4 nude mice inoculated with SCC117 cells developed tumors, indicating that the SCC117 cell line had the ability of xenogeneic tumorigenesis. The histopathological type of the transplanted tumor in nude mice was consistent with that of the primary tumor tissue, both of which were squamous cell carcinoma. The immunohistochemical results showed that in both human primary tumor and the transplanted tumor tissue in nude mice, RB, P53, and E-cadherin were all positive, P21 was weakly positive, while PTEN was negative. SCC117 was tested negative for the presence of HPV. STR sequence analysis showed that SCC117 cell line originated from primary tumor tissue and was not cross-contaminated by other cell lines. <b>Conclusions:</b> The human BMSCC cell line SCC117 was successfully established in China, which could provide a new experimental model for the study of oral SCC without HPV infection, especially BMSCC.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"59 9","pages":"942-949"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}