中华口腔医学杂志最新文献

{"title":"[Study on the invasion of <i>Prevotella intermedia</i> in tissues during carcinogenesis of oral mucosa].","authors":"T Liu, G Y Li, Z W Huang, X W Bu, J J Ma, N Duan, W M Wang, X Wang","doi":"10.3760/cma.j.cn112144-20241212-00476","DOIUrl":"10.3760/cma.j.cn112144-20241212-00476","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To explore the differences in bacterial communities within tissues during the process of oral mucosal carcinogenesis, and analyze the relationship between the high-abundance species &lt;i&gt;Prevotella intermedia&lt;/i&gt; (Pi) and the occurrence and development of oral mucosal carcinogenesis. &lt;b&gt;Methods:&lt;/b&gt; Fresh tissue samples were collected from patients diagnosed with oral leukoplakia (OLK), oral squamous cell carcinoma (OSCC), and healthy controls (HC) at Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, from January 2022 to November 2024, following strict inclusion criteria. Bacterial DNA was extracted from these specimens, and the 2bRAD sequencing for microbiome (2bRAD-M) was employed to analyze and compare the α and β diversity, as well as the community composition of bacteria within tissues, aiming to identify specifically expressed bacteria. Subsequently, paraffin-embedded clinical specimens were collected: 15 cases in the OLK group (including 4 cases of simple hyperplasia, 6 cases of mild dysplasia, and 5 cases of moderate to severe dysplasia), 12 cases in the OSCC group, and 5 cases in the HC group. A 4-nitroquinoline N-oxide (4NQO)-induced OLK progression mouse model was also constructed. Mice were randomly divided into three groups using a random number table, with six in each group. The negative control group was given distilled water to drink; Group 1 was given distilled water containing 4NQO to drink until week 12, while Group 2 was given distilled water containing 4NQO to drink until week 22. After the mice were sacrificed, their tongue tissue were collected and fixed. Fluorescence in situ hybridization (FISH) with specific probes was used to validate the presence of Pi in human and mouse tissue sections, analyzing the correlation between histopathological grading and the invasion depth of Pi. &lt;b&gt;Results:&lt;/b&gt; The 2bRAD-M microbial analysis revealed that the relative abundance of Pi in OSCC tissues (10.80%) was significantly higher than in the HC group (0.50%) (&lt;i&gt;P&lt;/i&gt;=0.001) and OLK group (0.70%) (&lt;i&gt;P&lt;/i&gt;=0.002). FISH probe detection showed that the fluorescence intensity of Pi in human OSCC tissues [123.50 (101.00, 142.30)] was higher than in the HC group [0.00 (0.00, 28.50)], simple hyperplasia OLK [0.00 (0.00, 35.25)], and mild dysplasia OLK [24.50 (0.00, 55.50)] groups, with statistically significant differences respectively (&lt;i&gt;P&lt;/i&gt;=0.002, &lt;i&gt;P&lt;/i&gt;=0.003, &lt;i&gt;P&lt;/i&gt;=0.005). However, there was no significant difference compared to moderate to severe dysplasia OLK [56.00 (28.00, 62.50)] (&lt;i&gt;P&lt;/i&gt;=0.210). The fluorescence area of Pi in human OSCC tissues [8 615.00 (7 439.00, 11 084.00)] was significantly larger than in the HC group [0.00 (0. 00, 45.00)], simple hyperplasia OLK group [0.00 (0.00, 81.00)], mild dysplasia [49.00 (0.00, 151.00)], and moderate to severe dysplasia groups [1 450.00 (454.00, 2 892.00)], with highly significant differences (&lt;i&gt;P&lt;/i&gt;&lt;0.001). There ","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 3","pages":"211-222"},"PeriodicalIF":0.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Key issues in the field of Temporomandibular Disorders and Occlusion from the topics of the first postgraduate debate competition].","authors":"H Li, N Jiang, S S Zhu","doi":"10.3760/cma.j.cn112144-20241206-00461","DOIUrl":"10.3760/cma.j.cn112144-20241206-00461","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 3","pages":"308-310"},"PeriodicalIF":0.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comprehensive analysis of the structural phenotypes and functional characteristics of B cells in oral lichen planus and oral lichenoid lesions through single-cell and spatial transcriptomics].","authors":"X J Yang, Y R Lai, X K Jiang, Y W Deng, L Pan, A N Dai, L Sun, Y F Wang, G Y Tang","doi":"10.3760/cma.j.cn112144-20241220-00500","DOIUrl":"10.3760/cma.j.cn112144-20241220-00500","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; Comprehensive characterization of B-cell phenotypes and spatial distribution in oral lichen planus (OLP) and related oral lichenoid lesions (OLL)(OLP/OLL), with an emphasis on transcriptomic profiling and functional analysis, to uncover the epigenetic mechanisms underlying B cell-mediated immune regulation within the oral mucosal microenvironment. &lt;b&gt;Methods:&lt;/b&gt; Single-cell RNA sequencing raw data were sourced from the GSE211630 database, encompassing samples from 2 cases of erosive OLP (EOLP), 3 cases of non-erosive OLP (NEOLP) and 1 healthy control (NORMAL). Following stringent quality control, the data underwent normalization, selection of highly variable genes and batch effect correction. Subsequent analyses included dimensionality reduction and unsupervised clustering to identify distinct cell populations. This study collected pathological specimens from 3 OLP/OLL patients and 3 healthy controls who were treated at the Department of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from January 2021 to December 2023. Using 10X Genomics Visium HD spatial transcriptomics technology, tissue sections were processed through dewaxing, staining and histological imaging, enabling the reconstruction of nucleic acid structures and the capture of gene expression profiles. Data analysis included quality assessment, gene quantification, normalization, dimensionality reduction and clustering. Furthermore, cell type deconvolution was performed using the robust cell type decomposition algorithm, integrating single-cell transcriptomic data to accurately predict and spatially resolve cell type distributions within the tissue microenvironment. &lt;b&gt;Results:&lt;/b&gt; After integrating single-cell data from EOLP, NEOLP and NORMAL, cells were classified into seven major categories: B/plasma cells, endothelial cells, epithelial cells, fibroblasts, myeloid cells, smooth muscle cells and T/natural killer cells. The proportion of B/plasma cells varied significantly among the three groups, accounting for 10.7% (1 693/15 815), 3.8% (833/21 653) and 0.4% (47/11 556) of the total cells respectively. Further clustering analysis of B/plasma cells identified four distinct subpopulations: naive B cells, activated B cells, memory B cells and plasma cells. In the EOLP group, these subpopulations constituted 25.9% (348/1 344), 45.9% (617/1 344), 3.3% (45/1 344) and 24.9% (334/1 344) of the B/plasma cells respectively. In the NEOLP group, they represented 31.6% (195/617), 59.6% (368/617), 0.2% (1/617) and 8.6% (53/617). Howerer, only plasma cells were detected in the NORMAL group. Spatial analysis revealed that B cells were actively involved in the formation of tertiary lymphoid structures (TLS) at various stages in OLP/OLL samples, with a prominent structural organization observed in secondary follicle-like TLS. Within these structures, the expressions of T cells marker gene CD3E and B cells marker gene MS4A1 wer","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 3","pages":"201-210"},"PeriodicalIF":0.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Oral localized scleroderma: case study].","authors":"S X Jiang, Q Han, X Zeng, X B Luo, Q M Chen","doi":"10.3760/cma.j.cn112144-20241214-00479","DOIUrl":"10.3760/cma.j.cn112144-20241214-00479","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 3","pages":"282-286"},"PeriodicalIF":0.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A humble opinion on the advance of oral medicine research in China].","authors":"X Zeng, Q M Chen","doi":"10.3760/cma.j.cn112144-20250126-00033","DOIUrl":"10.3760/cma.j.cn112144-20250126-00033","url":null,"abstract":"<p><p>To better reflect the current status and key research trends in oral medicine research of China and to enhance the overall research abilities in this field, this special issue on oral medicine research integrates the work of several young academic leaders from oral medicine research centers nationwide. Their work focuses on key areas such as artificial intelligence-assisted diagnosis, the pathogenic mechanisms of intracellular bacteria, the relations between oral mucosal diseases and systemic diseases, as well as the study and reporting of rare and unique cases, aligning with the forefront of biomedical research. Although it does not encompass all the high-level researches conducted in China, it presents a rich and diverse array of academic achievements. Those achievements of researches reflect that it is important to further advance interdisciplinary and cross-disciplinary research collaborations, particularly by strengthening cooperation with engineering and technological disciplines in the future. We also need to set sights on driving researches in novel pathogenesis mechanisms of oral mucosal diseases, exploring innovative diagnostic technologies, enhancing diagnostic accuracy and treatment efficacy, and developing new effective intervention strategies. Additionally, greater emphases will be placed on ensuring the accuracy, comprehensiveness, readability, and scientific rigor of case studies and reports to promote sustained progress in this field.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 3","pages":"197-200"},"PeriodicalIF":0.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preliminary study on the significance of serum thyroid antibody in the selection of treatment for oral lichen planus].","authors":"C X Liu, F M Chen, S S Zhang, F Tang, S J Zhang, Y Qian, Q M Chen","doi":"10.3760/cma.j.cn112144-20241216-00487","DOIUrl":"10.3760/cma.j.cn112144-20241216-00487","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the efficacy of different treatment of oral lichen planus (OLP) patients with thyroid antibody positive and its correlation with thyroid antibody, providing more targeted treatment for OLP patients with thyroid antibody positive. <b>Methods:</b> Patients who were admitted to Department of Oral Medicine, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine for OLP with serum thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody (TGAb) from February 2020 to June 2024 were included. Demographic and clinical data were recorded, and qualitative status and quantitative titers of TPOAb/TGAb were collected. TPOAb and/or TGAb positive patients were included into the thyroid antibody positive group, while patients with both TPOAb and TGAb negative were included into the thyroid antibody negative group. According to the treatment methods, they were divided into topical treatment group, topical treatment combined with oral immunosuppressant group, and topical treatment combined with oral immunoenhancer group. After 2 weeks and 1 month of treatment, the clinical efficacy of thyroid antibody positive group and negative group in OLP patients under different treatment methods were compared, and the correlations of different clinical efficacy with age, sex and thyroid antibody titer were analyzed. <b>Results:</b> A total of 116 OLP patients were included in this study, of which 38 (32.8%) were thyroid antibody positive and 78 (67.2%) were thyroid antibody negative. Compared with untreated, the modified OLP score and pain degree in thyroid antibody negative group were significantly improved after 2 weeks and 1 month of topical treatment and topical treatment combined with oral immunosuppression (<i>P<</i>0.01). Although the thyroid antibody positive group also showed a remission trend, there was no statistical significance before and after treatment (<i>P></i>0.05). For topical treatment combined with oral immunoenhancer, the modified OLP score and pain degree in both groups of antibody positive and negative were statistically significant different after 2 weeks and 1 month of treatment compared with before (<i>P<</i>0.01), while there was no statistically significant difference between the two groups (<i>P></i>0.05). Multivariate Logistic regression analysis showed that the treatment efficacy of OLP in topical treatment group, topical treatment combined with oral immunosuppressant group, and topical treatment combined with oral immunoenhancer group had no significant correlation with age, sex, as well as the TPOAb and TGAb titer (<i>P></i>0.05). <b>Conclusions:</b> Thyroid antibody TPOAb and TGAb could be reference factors for the selection of OLP treatment plan. For patients of OLP with positive thyroid antibodies, topical therapy combined with oral immunoenhancers may be preferred.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 3","pages":"223-231"},"PeriodicalIF":0.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of multimodal medical image fusion in temporomandibular joint disease].","authors":"W Zhao, X R Sun, Y Feng, W C Liu","doi":"10.3760/cma.j.cn112144-20241217-00495","DOIUrl":"10.3760/cma.j.cn112144-20241217-00495","url":null,"abstract":"<p><p>With the rapid development of modern technology, the deep integration of computer science and medicine has greatly propelled the field of the dental diagnostics field. Multimodal medical image registration refers to the fusion of information from various modalities of medical imaging. In other words, with the assistance of computer technology, the image data acquired from various radiological examinations undergo spatial registration and are merged to create a comprehensive new image, serving the purpose of computer-aided diagnosis. The temporomandibular joint (TMJ) has complex anatomical structures, so there is a wide range of diseases associated with it. Traditional single-modal imaging methods can only provide partial anatomical information, which limits the comprehensive assessment and subsequently hinders the diagnosis and treatment of TMJ disease. Multimodal medical image registration techniques, owing to their ability to provide comprehensive information, have been widely applied in the field of dentistry. This has greatly enhanced the efficiency of diagnosing and treating TMJ disease. The article discusses the advancements in the application of multimodal medical image registration in the context of temporomandibular joint disease.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 3","pages":"296-301"},"PeriodicalIF":0.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Artificial light at night effects glucose metabolism in the developing jawbone by inhibiting melatonin secretion].","authors":"Q M Tang, Y Yin, L L Chen","doi":"10.3760/cma.j.cn112144-20240927-00361","DOIUrl":"10.3760/cma.j.cn112144-20240927-00361","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the effects of artificial light at night on the growth of mandibles in mice and its regulatory mechanisms. &lt;b&gt;Methods:&lt;/b&gt; A mouse model of artificial light at night (night light pollution group) and normal lighting (normal light group) was established by controlling light exposure time, with 4 mice in each group. Micro-CT was employed to analyze the differences in bone quantities of the mandibles between the two groups. Real-time fluorescence quantitative PCR (RT-qPCR) was used to examine the expression levels of osteogenic differentiation and metabolism-related genes in the cortical bone and condylar ossification center of the mandibles. Enzyme-linked immunosorbent assay was utilized to assess the diurnal variation of serum melatonin concentrations between the two groups. The artificial light at night experimental group received daily timed injections of a defined dose of melatonin to restore the diurnal variation of serum melatonin concentration in the mice, while the normal light group and the artificial light at night control group received the same volume of saline. Bone quantities, mandibular tissue morphologies, ossification differentiation in the condylar region and cortical bone, as well as glucose metabolism expression differences were assessed across the three groups. &lt;b&gt;Results:&lt;/b&gt; The cortical bone thickness of the mandibles in the artificial light at night group [(0.196±0.017) mm] was significantly less than that in the control group [ (0.228±0.007) mm] (&lt;i&gt;P&lt;/i&gt;=0.029). The bone volume fraction of the condylar ossification center in the artificial light at night group [(36.90±1.09) %] was significantly lower than that in the normal light group [(54.24±1.49) %] (&lt;i&gt;P&lt;/i&gt;&lt;0.001). The length of the mandible in the artificial light at night group [(10.86±0.17) mm] was significantly shorter than that in the normal light group [(11.41±0.32) mm] (&lt;i&gt;P&lt;/i&gt;=0.032). RT-qPCR results indicated that the expressions of osteogenic-related genes alkaline phosphatase, liver/bone/kidney (Alpl), osteocalcin (Ocn), Runt-related transcription factor 2 (Runx2), and osterix (Osx) in the cortical bone and condylar ossification center of mice in the artificial light at night group were significantly lower than those in the normal light group (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). The expression of metabolism-related genes protein kinase, AMP activated alpha 1 (Prkaa1), V-type proton ATPase subunit d1 (Atp6v0d1), and cytochrome C oxidase subunit Ⅳ isoform 1 (Cox4i1) in the cortical bone and condylar ossification center of mice in the artificial light at night group were also significantly reduced compared to normal mice (all &lt;i&gt;P&lt;/i&gt;&lt;0.01). The serum melatonin concentration peaked 8 hours after lights off in the normal light group, whereas the artificial light at night group exhibited a significantly reduced nocturnal serum melatonin concentration with no apparent peak compared to the normal light group. Micro-CT results demonstrated ","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 2","pages":"140-150"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of influencing factors on the height of gingival papilla around implants].","authors":"D D Feng, M Z Wang, F Liu, Y L Zhan, Y Li","doi":"10.3760/cma.j.cn112144-20240725-00292","DOIUrl":"10.3760/cma.j.cn112144-20240725-00292","url":null,"abstract":"<p><p>The anatomical structure of the gingival papilla around the implant is special, and it is difficult to recover after injury. The reduction of its height will have a negative impact on function and esthetics, and it is one of the many problems in implant treatment at present. This paper analyzes the influencing factors of implant gingival papilla height from three aspects: anatomical factors, implant surgical design and implant restorative design, including the classical influencing factors such as the shape of natural teeth, interproximal distance between the implant and the adjacent tooth, soft tissue grafting, and contour of implant restorations, as well as the hot and controversial influencing factors in recent years such as the thickness of soft tissues around the implant, the implant surgical timing, the flap design, and the surgical incision, in order to provide reference for clinicians in the process of implant treatment for gingival papilla preservation and reconstruction.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 2","pages":"172-178"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of osteon-like concentric microgroove structures of different sizes on the osteoclastic differentiation of macrophages].","authors":"X Z Weng, C Huang, Y Z Lai, G Yin, Y X Lei, Z Q Xu","doi":"10.3760/cma.j.cn112144-20240804-00305","DOIUrl":"10.3760/cma.j.cn112144-20240804-00305","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the effect of the sizes of osteon-like concentric microgroove structures on the osteoclastic differentiation of macrophages on titanium surfaces, and to provide reference for the surface modification of implants. &lt;b&gt;Methods:&lt;/b&gt; The silicon wafers sputtered with titanium were selected as the control group (smooth surface specimens) and four concentric groups (concentric circles with the maximum diameter of 200 μm, the minimum diameter of 20 μ m, the spacing of concentric circles of 10 or 30 μm, the width of microgrooves of 10 or 30 μm, and the depth of microgrooves of 5 or 10 μm) specimens (the total sample size in each group was 27). The width of microgrooves of C10-5 and C10-10 groups was 10 μm, the depth was 5 and 10 μm, and the width of microgrooves of C30-5 and C30-10 groups was 30 μ m, the depth was 5 and 10 μ m, respectively. The physicochemical properties of the material surfaces were characterized using scanning electron microscopy and contact-angle measurement. The proliferation, adhesion of macrophage-like cell line RAW264.7 and the formation of osteoclast actin-rings on the specimen surfaces were observed by cell counting kit-8 (CCK-8), immunofluorescence staining and laser confocal microscopy. Tartrate resistant acid phosphatase (TRAP) quantitative detection, real-time fluorescence quantitative PCR (RT-qPCR) and Western blotting were used to investigate the regulation of osteon-like concentric microgroove structures on the specimen surfaces on the osteoclastic differentiation of macrophages. &lt;b&gt;Results:&lt;/b&gt; Macrophages aggregated and grew disorderly on the surface of the smooth group, and arranged in concentric circles along the microgroove structures on the surfaces of the concentric groups. After 5 days of culture, the cell proliferation of C30 groups (the &lt;i&gt;A&lt;/i&gt; values of C30-5 group and C30-10 group were 1.335±0.018 and 1.340±0.033, respectively) was significantly higher than that of C10 groups (the &lt;i&gt;A&lt;/i&gt; values of C10-5 group and C10-10 group were 0.967±0.015 and 1.182±0.020, respectively)(all &lt;i&gt;P&lt;/i&gt;&lt;0.05). The cell proliferation of the four concentric groups was significantly higher than that of the control group (the &lt;i&gt;A&lt;/i&gt; value was 0.796±0.012), with statistical significance (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). After osteoclastic induction for 5 days the osteoclasts induced in the C10-5 and C10-10 groups exhibited smaller actin rings and fewer numbers. The TRAP activity in each concentric group was significantly lower than that in the control group (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The expression levels of osteoclast differentiation-related genes TRAP (0.610±0.022) in the C10-10 group was lowest, and CtsK (0.489±0.136, 0.445±0.037) in the C10-5 and C10-10 groups were lower compared to the smooth group and other concentric groups, with statistical significance (all &lt;i&gt;P&lt;/i&gt;&lt;0.05), the expression levels of osteoclast differentiation-related proteins TRAP (0.648±0.041), MMP-9 (0.688±0.026) in the C10-10 group wer","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 2","pages":"160-168"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}