中华口腔医学杂志最新文献

{"title":"[A 10-year follow-up of multidisciplinary combined treatment for severe periodontitis: a case report].","authors":"R Cao, L Yang, N Xu, Z Y Xiong, S M Peng, L Ma, Z G Cao","doi":"10.3760/cma.j.cn112144-20241219-00499","DOIUrl":"10.3760/cma.j.cn112144-20241219-00499","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"534-539"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Artificial intelligence-based multimodal fusion diagnosis: advances in precision diagnosis of periodontitis].","authors":"Z Chai, Y Li, M L You, H N Song, F Xu, A Li","doi":"10.3760/cma.j.cn112144-20250110-00009","DOIUrl":"10.3760/cma.j.cn112144-20250110-00009","url":null,"abstract":"<p><p>Periodontitis is a globally prevalent inflammatory oral disease, affecting approximately 50% of the population worldwide and imposing a substantial burden on patients' health and quality of life. Early and accurate diagnosis is critical for preventing disease progression; however, conventional diagnostic approaches often rely on subjective clinical assessments, which only primarily evaluate the cumulative state of the disease, thus limiting their ability to achieve precise early detection. In recent years, the rapid advancement of artificial intelligence (AI) in medical diagnostics has demonstrated significant promise, particularly through the integration of multimodal data to enable more comprehensive information capture and analysis. Multimodal data fusion, which combines diverse inputs such as imaging, clinical parameters, and biomarkers, offers transformative potential for AI-powered periodontitis diagnostics. This innovative approach aims to overcome the limitations of traditional methods, significantly enhancing diagnostic accuracy and predictive capabilities. This manuscript reviews the primary diagnostic techniques for periodontitis, explores recent advances in AI applications within this domain, and emphasizes the potential of multimodal data in facilitating precision diagnosis. Furthermore, it provides new insights and supports for personalized treatment strategies.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"558-566"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on injury of vascular intima with hemostatic clamp and optimization of protective strategy].","authors":"L Z Ren, Q Q Wang, D Wang, Z P Zhang, X F Wu, R Sun","doi":"10.3760/cma.j.cn112144-20241231-00510","DOIUrl":"10.3760/cma.j.cn112144-20241231-00510","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To explore the intimal injury of two kinds of hemostatic clips under different clamping forces, and to optimize and verify the calculation formula of minimum occlusion force (MOF), so as to provide theoretical basis for selecting appropriate hemostatic clips to reduce vascular injury. &lt;b&gt;Methods:&lt;/b&gt; A total of 96 male SD rats with body weight ranging from 280 to 300 g (Animal Experimental Center of Shanxi Medical University) were randomly assigned to metal hemostatic clip group (&lt;i&gt;n&lt;/i&gt;=48) and disposable hemostatic clip group (&lt;i&gt;n&lt;/i&gt;=48) by random number table method. Each group was further divided into four subgroups with clamping forces of 0.3, 0.6, 0.9 and 1.2 N for abdominal aortic injury experiments. The damage to the vascular intima was observed under a scanning electron microscope. A vascular closure model was established for mathematical analysis to derive the theoretical calculation formula of the arterial blood management factor (MOF). According to blood pressure (BP), blood vessel diameter (D) and hemostatic clamp width (W), the average values of theoretical and actual MOF data were analyzed by paired &lt;i&gt;t&lt;/i&gt; test to verify the accuracy of the formula. From January 2021 to December 2022, six patients (with 12 branch arteries) who presented with oral and maxillofacial malignancies at the Department of Oral and Maxillofacial Surgery, Shanxi Medical University School and Hospital of Stomatology, were included to validate the accuracy of the MOF formula. &lt;b&gt;Results:&lt;/b&gt; Under electron microscopy, when the clamping force was 0.3 N, the one-time hemostatic clip caused very little damage to the vascular intima (grade 1 injury), with only a few folds being flattened. When the clamping force was 0.6 N, both types of hemostatic clips caused partial peeling of the intima surface (grade 2-3 injury). However, when the clamping force was adjusted to 0.9 and 1.2 N, the damage caused by both types of hemostatic clips was more severe (grade 3-4 injury), with large areas of intima peeling or even disappearance. In some specimens of the metal hemostatic clip group, the damage even reached the muscular layer. Vascular closure model analysis showed that MOF=2×(1/2×π×D×W×BP), the mean diameter of abdominal aorta of 24 SD rats was (1.41±0.07) mm, the blood pressure was (83.29±11.56) mmHg (1 mmHg=0.133 kPa). The theoretical MOF value was (0.096±0.015) N, the actual clamping force (ACF) was (0.095±0.012) N, there was no statistical significance between the two groups (&lt;i&gt;t&lt;/i&gt;=-0.35, &lt;i&gt;P&lt;/i&gt;=0.725). In clinical application, the MOF calculation formula was used to select the appropriate hemostatic clip for 12 arteries, successfully blocking the blood flow, verifying the accuracy of the formula. &lt;b&gt;Conclusions:&lt;/b&gt; Hemostatic clips can cause damage to the inner membrane of blood vessels, and the greater the clamping force, the more severe the damage. Disposable hemostatic clips have certain advantages in avoiding excessive damage t","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"501-508"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Intentional replantation to preserve natural teeth in patients with severe periodontitis: a case report].","authors":"X Y Zhang, J Y Zhou, C C Ye, P Huang, L Zhao","doi":"10.3760/cma.j.cn112144-20241218-00497","DOIUrl":"10.3760/cma.j.cn112144-20241218-00497","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"553-557"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of lipopolysaccharide-activated interferon gene stimulator signaling on periodontal ligament cells].","authors":"Y Zhou, D L Wang, H Y Cai, R Zhang, Y Xu","doi":"10.3760/cma.j.cn112144-20250211-00043","DOIUrl":"10.3760/cma.j.cn112144-20250211-00043","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the effects of lipopolysaccharide (LPS)-activated stimulator of interferon genes (STING) signaling on the biological behavior of periodontal ligament cells and its mechanism of action. &lt;b&gt;Methods:&lt;/b&gt; Human periodontal ligament cells (hPDLC) were divided into the PBS group and the LPS group by stimulated with phosphate-buffered saline (PBS) and LPS derived from &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt; (ATCC 33277) for 12 hours, respectively. The intracellular distribution of 8-hydroxydeoxyguanosine (8-OHdG), a marker of DNA damage, and the activation level of STING signaling were detected by immunofluorescence. The source of intracellular double-stranded DNA was detected by live-cell probes. The levels of osteogenic-related proteins, such as special protein 7 (SP7), bone morphogenetic protein 2 (BMP2), cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS), and STING were detected by Western blotting. The cell supernatants of the PBS group and the LPS group were collected, and the levels of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and interferon (IFN)-β, were detected by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). A total of 12 cgas knockout mice and 12 littermate wild-type mice were constructed. The maxillary second molars of the mice were ligated with silk or sham surgery, respectively. After 7 days of modeling, the mice were divided into littermate control sham surgery group, littermate control periodontitis group, cgas knockout sham surgery group, and cgas knockout periodontitis group, with 6 mice in each group. Micro-CT was used to collect image data, and three-dimensional reconstructions were performed on the maxillary samples of each group. The distance from the cemento-enamel junction to the alveolar bone crest (CEJ-ABC), bone volume fraction (BV/TV), and bone mineral density (BMD) in the model area were statistically analyzed using CTAn and CTVOX software. Frozen sectioning was used to obtain sections of the maxillary molars of each group of mice, and the signal intensities of cGAS and STING proteins were detected by immunofluorescence. &lt;b&gt;Results:&lt;/b&gt; Immunofluorescence results showed that the fluorescence signal intensity of 8-OHdG outside the nucleus in the LPS group (4.09±0.24) was significantly higher than that in the PBS group (1.00±0.10) (&lt;i&gt;t=&lt;/i&gt;20.33, &lt;i&gt;P&lt;&lt;/i&gt;0.001). The co-localization signal of mitochondrial marker TOM20 and 8-OHdG (8.56±0.53) were significantly higher than that of PBS group (1.00±0.09) (&lt;i&gt;t=&lt;/i&gt;24.37, &lt;i&gt;P&lt;&lt;/i&gt;0.001). Live cell DNA probe detection showed that the signal intensity of double-stranded DNA in LPS group (3.23±0.12) was significantly stronger than that in PBS group (1.00±0.17) (&lt;i&gt;t=&lt;/i&gt;18.30, &lt;i&gt;P&lt;&lt;/i&gt;0.001). Immunofluorescence demonstrated a significant increase in STING expression in hPDLC of the LPS group (&lt;i&gt;t=&lt;/i&gt;6.42, &lt;i&gt;P&lt;&lt;/i&gt;0.001), and it was colocalized with the Golgi marker GM130. ELISA results sh","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"492-500"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A 9-year longitudinal observation of multidisciplinary treatment of stage Ⅳ grade C molar-incisor pattern periodontitis: a case report].","authors":"H M Guo, X J Wang, Q F Sun, S H Ge","doi":"10.3760/cma.j.cn112144-20241217-00490","DOIUrl":"10.3760/cma.j.cn112144-20241217-00490","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"540-545"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment and verification of nomogram model for predicting implant-assisted bone grafting after posterior teeth alveolar ridge preservation].","authors":"J Q Deng, Z Yang, Y Liu, R Y Cao, Y P Pan","doi":"10.3760/cma.j.cn112144-20241212-00475","DOIUrl":"10.3760/cma.j.cn112144-20241212-00475","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; Constructing a risk prediction model to assess the impact of various factors on the need for auxiliary bone grafting with implant placement following alveolar ridge preservation (ARP) in posterior teeth. &lt;b&gt;Methods:&lt;/b&gt; According to the sample size calculation formula, the sample size was calculated using the pmsampsize package of R 4.1.3 software, based on inclusion and exclusion criteria, a total of 110 posterior teeth in 98 patients who underwent ARP at the Department of Periodontology, School and Hospital of Stomatology, China Medical University, from January 2018 to May 2024 were conducted. Teeth were randomly divided into modeling group and validation group with 7∶3 ratio according to the random number table. The modeling group was divided into direct implantation group and auxiliary bone grafting group on the basis of whether auxiliary bone grafting was performed 6 months after ARP. Univariate and multivariate analyses were conducted to identify factors influencing auxiliary bone grafting with implant placement following ARP. Nomogram was constructed using R software. Receiver operator characteristic (ROC) curve and calibration curve were drawn to evaluate model differentiation and consistency. The decision curve analysis (DCA) was used to assess the clinical application value of the model. &lt;b&gt;Results:&lt;/b&gt; Age (&lt;i&gt;OR&lt;/i&gt;=1.06, &lt;i&gt;P&lt;/i&gt;=0.001), maximum attachment loss (AL) (&lt;i&gt;OR&lt;/i&gt;=1.75, &lt;i&gt;P&lt;/i&gt;&lt;0.001), reason of tooth extraction (&lt;i&gt;OR&lt;/i&gt;=12.73, &lt;i&gt;P&lt;/i&gt;&lt;0.001), smoking [&lt;10 cigarettes/d (&lt;i&gt;OR&lt;/i&gt;=7.59, &lt;i&gt;P&lt;/i&gt;&lt;0.001);≥10 cigarettes/d (&lt;i&gt;OR&lt;/i&gt;=28.12, &lt;i&gt;P&lt;/i&gt;&lt;0.001)] and stage of periodontitis [stage Ⅱ (&lt;i&gt;OR&lt;/i&gt;=2.57, &lt;i&gt;P&lt;/i&gt;=0.430); stage Ⅲ (&lt;i&gt;OR&lt;/i&gt;=21.00, &lt;i&gt;P&lt;/i&gt;=0.007); stage Ⅳ (&lt;i&gt;OR&lt;/i&gt;=76.50, &lt;i&gt;P&lt;/i&gt;&lt;0.001)] influenced the necessity for auxiliary bone grafting with implant placement after ARP. After multivariate analysis of the above influencing factors, it was found that smoking [&lt;10 cigarettes/d (&lt;i&gt;OR&lt;/i&gt;=7.02, &lt;i&gt;P&lt;/i&gt;=0.009);≥10 cigarettes/d (&lt;i&gt;OR&lt;/i&gt;=10.27, &lt;i&gt;P&lt;/i&gt;=0.026)] was an independent risk factor for the need of auxiliary bone grafting with implant placement after ARP. The area under the ROC curve for internal verification was 0.90 (95&lt;i&gt;%CI&lt;/i&gt;: 0.84-0.97), and the H-L goodness of fit test results were χ&lt;sup&gt;2&lt;/sup&gt;=4.79, &lt;i&gt;P&lt;/i&gt;=0.780, indicating a good agreement. The area under the externally verified ROC curve was 0.97 (95&lt;i&gt;%CI&lt;/i&gt;: 0.92-1.00), suggesting that the fitting effect was slightly lower than that of the modeling group, and the predicted value of the model was slightly lower than the true value, which might underestimate the risk of additional surgery in patients. &lt;b&gt;Results:&lt;/b&gt; of H-L goodness of fit test were χ&lt;sup&gt;2&lt;/sup&gt;=5.03, &lt;i&gt;P&lt;/i&gt;=0.754. The DCA curve showed that when the probability of high-risk threshold was between 0.06 and 0.93, the clinical application value of the prediction model was higher. &lt;b&gt;Conclusions:&lt;/b&gt; Age, smoking, reason of tooth extracti","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"464-473"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research on surface modification of pure titanium with histatin coating and its effects on adhesion and spreading of mouse bone marrow mesenchymal stem cells].","authors":"S Wang, B Y Chen, L Zhao, C Liu","doi":"10.3760/cma.j.cn112144-20241024-00398","DOIUrl":"10.3760/cma.j.cn112144-20241024-00398","url":null,"abstract":"<p><p><b>Objective:</b> To prepare histatin 1 (Hst 1) and D-histatin 1 (D-Hst 1) coatings on pure titanium surfaces and to evaluate their impact on the initial adhesion and spreading of mouse bone marrow mesenchymal stem cells (mBMSC). <b>Methods:</b> The test pieces were divided into four groups: pure titanium, piranha solution-treated, 3-aminopropyltriethoxysilane-treated, Hst 1 modified groups or D-Hst 1 modified groups, with five pieces in each group. The surface hydrophilicity of the test pieces was detected by contact angle measurement. The surface microtopography was observed using atomic force microscopy (AFM), and the surface elemental composition was analyzed by X-ray photoelectron spectroscopy (XPS). The modification of Hst 1 on the titanium surface and the early adhesion and spreading of the third-generation normal mBMSC on Hst 1/D-Hst 1 modified group were observed using confocal laser scanning microscopy (CLSM). <b>Results:</b> The Hst 1 modified group had better surface hydrophilicity (contact angle 51.63°±3.80°) compared to the pure titanium group (contact angle 60.36°±4.38°). AFM observations revealed shallow mechanical scratches on the surfaces of all test pieces. CLSM and XPS elemental analysis confirmed the successful modification of Hst 1 on the titanium surface. Compared with the pure titanium group, both Hst 1 and D-Hst 1 could promoted the early adhesion of mBMSC. The Hst 1 modified group promoted the spreading of mBMSC after early adhesion, while the D-Hst 1 modified group showed no statistically significant difference in promoting mBMSC spreading compared to the pure titanium group (<i>P</i>>0.05). <b>Conclusions:</b> The modification of Hst 1/D-Hst 1 on pure titanium surfaces using Schiff base reaction is effective. It is conducive to the early adhesion and spreading of mBMSC.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"518-524"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Feasibility study on early orthodontic intervention in stage Ⅳ periodontitis].","authors":"J Q Bao, L H Lei, Z X Wang, Y M Feng, W L Sun, L L Chen","doi":"10.3760/cma.j.cn112144-20241205-00458","DOIUrl":"10.3760/cma.j.cn112144-20241205-00458","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To compare the effects of early orthodontic intervention and conventional sequential periodontal-orthodontic treatment to periodontal health in patients with stage Ⅳ periodontitis. &lt;b&gt;Methods:&lt;/b&gt; A total of 30 patients with stage Ⅳ periodontitis, who underwent combined periodontal and orthodontic therapies at the Department of Periodontology, the Second Affiliated Hospital of Zhejiang University School of Medicine, from January 2018 to August 2024, were included. Patients who underwent early orthodontic intervention were initiated simultaneously or within one month after supragingival scaling and subgingival root planning (&lt;i&gt;n&lt;/i&gt;=15). While patients in control group accomplished supragingival scaling, subgingival root planning, and corresponding periodontal surgeries to achieve inflammation control before starting orthodontic treatment (&lt;i&gt;n&lt;/i&gt;=15). Periodontal parameters, including clinical attachment loss (CAL), probing depth (PD), and bleeding on probing (+) % [BOP (+) %], were measured at baseline, one year after orthodontic treatment, and at the end of combined periodontal-orthodontic therapy respectively. Improvements in periodontal parameters and differences in tooth loss between the two groups were compared. &lt;b&gt;Results:&lt;/b&gt; After receiving combined periodontal-orthodontic treatment, the CAL of the early orthodontic intervention group significantly decreased from (4.39±0.90) mm before treatment to (2.41±0.35) mm at the end of treatment (&lt;i&gt;t&lt;/i&gt;=7.92, &lt;i&gt;P&lt;/i&gt;&lt;0.001). Similarly, the PD significantly reduced from (4.20±1.04) mm before treatment to (2.20±0.38) mm at the end of treatment (&lt;i&gt;t&lt;/i&gt;=7.01, &lt;i&gt;P&lt;/i&gt;&lt;0.001). The BOP(+)% also showed a significant improvement, decreasing from 89.29% (68.00%, 100.00%) before treatment to 13.04% (7.14%, 17.86%) at the end of treatment (&lt;i&gt;Z&lt;/i&gt;=-3.41, &lt;i&gt;P&lt;/i&gt;&lt;0.001). There were no statistically significant differences between the early orthodontic intervention group and control group in terms of baseline mean CAL, mean PD, and BOP(+)% (&lt;i&gt;t&lt;/i&gt;=1.30, &lt;i&gt;P&lt;/i&gt;=0.205; &lt;i&gt;t&lt;/i&gt;=1.28, &lt;i&gt;P&lt;/i&gt;=0.212; &lt;i&gt;Z&lt;/i&gt;=0.58, &lt;i&gt;P&lt;/i&gt;=0.559). Furthermore, the improvements in CAL and PD between the two groups were not significantly different compared to baseline (&lt;i&gt;Z&lt;/i&gt;=-1.10, &lt;i&gt;P&lt;/i&gt;=0.272; &lt;i&gt;Z&lt;/i&gt;=-0.93, &lt;i&gt;P&lt;/i&gt;=0.351). However, the number of missing teeth was significantly lower in the early orthodontic intervention group than in the control group (χ²=3.96, &lt;i&gt;P&lt;/i&gt;=0.047). The duration of combined periodontal-orthodontic treatment in the early orthodontic intervention group was [33.13 (23.37, 36.20) months], which was significantly shorter than that in the control group [37.47 (32.33, 50.90) months] (&lt;i&gt;Z&lt;/i&gt;=2.07, &lt;i&gt;P&lt;/i&gt;=0.037). &lt;b&gt;Conclusions:&lt;/b&gt; Both early orthodontic intervention and conventional periodontal-orthodontic treatment significantly improved CAL, PD, and BOP(+)% in stage Ⅳ periodontitis patients. Early orthodontic intervention contributed to the preservation of ","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"474-481"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Inhibition of osteogenic differentiation of mouse bone marrow mesenchymal stem cells and maxillary expansion osteogenesis by cytoskeleton-associated protein 4 knockout].","authors":"H Z Wang, D H Tao, S P Chang, X N He, B Li, Y M Zhao","doi":"10.3760/cma.j.cn112144-20241009-00376","DOIUrl":"10.3760/cma.j.cn112144-20241009-00376","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the effect of cytoskeleton-associated protein 4 (CKAP4) gene knockout on maxillary expansion osteogenesis and its regulatory mechanism on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSC). &lt;b&gt;Methods:&lt;/b&gt; Nineteen wild type (WT) and nineteen CKAP4 gene knockout (Ckap4&lt;sup&gt;-/-&lt;/sup&gt;) mice aged 6-8 weeks were selected to establish a mouse model of rapid maxillary expansion. Samples were taken on the 7th and 14th day after the operation. Micro-CT and HE staining were used to evaluate bone regeneration. Tissue proteins in the modeled area were collected, and Western blotting analysis (WB) was used to detect the protein expression levels of alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN). BMSC were isolated from WT and Ckap4&lt;sup&gt;-/-&lt;/sup&gt; mice. The expression of surface markers CD29, Sca-1, CD44, CD45, CD34, and CD11b was detected by flow cytometry, and cell proliferation ability was detected by 5-ethynyl-2'-deoxyuridine (EdU). After 7 days of osteogenic induction, real-time fluorescence quantitative PCR (RT-qPCR) and WB were used to detect the expression levels of RUXN2, ALP, OCN, protein kinase B (AKT), and phosphorylated protein kinase B (p-AKT). After 21 days, alizarin red staining and cetyl pyridine chloride quantification were used to detect the differences in mineralized nodule formation in each group. In CKAP4 gene knockout BMSC, the small-molecule AKT agonist sc79 (4 μg/ml) was added as the intervention group (Ckap4&lt;sup&gt;-/-&lt;/sup&gt; +sc79), and dimethyl sulfoxide (DMSO) treatment was used as the control group (Ckap4&lt;sup&gt;-/-&lt;/sup&gt; +DMSO). After osteogenic induction, RT-qPCR, WB, and alizarin red staining were used to compare the osteogenic differentiation differences between the two groups of cells. &lt;b&gt;Results:&lt;/b&gt; The micro-CT results showed that at 7 days and 14 days after surgery, the new bone volume in the Ckap4&lt;sup&gt;-/-&lt;/sup&gt; group [(0.070±0.010) and (0.146±0.019) mm&lt;sup&gt;3&lt;/sup&gt;] was significantly lower than that in the WT group [(0.094±0.006) and (0.196±0.013) mm&lt;sup&gt;3&lt;/sup&gt;] (both &lt;i&gt;P&lt;/i&gt;&lt;0.01). HE-stained histological sections showed that the area of new bone tissue in the Ckap4&lt;sup&gt;-/-&lt;/sup&gt; group at 7 days and 14 days after surgery [(0.101±0.008) and (0.158±0.010) mm&lt;sup&gt;2&lt;/sup&gt;] was also significantly lower than that in the WT group [(0.116±0.005) and (0.183±0.008) mm&lt;sup&gt;2&lt;/sup&gt;] (both &lt;i&gt;P&lt;/i&gt;&lt;0.05). WB was used to detect the tissue proteins in the maxillary modeling area of mice in the two groups 7 days after surgery. The results showed that the expression levels of ALP, RUNX2 and OCN in the Ckap4&lt;sup&gt;-/-&lt;/sup&gt; group were significantly lower than those in the WT group. BMSC from wild-type mice and CKAP4 knockout mice were both positively expressed for CD29, CD44, and Sca-1, and basically not expressed for CD45, CD34, and CD11b. EdU assay showed that there was no significant difference in the proliferation ability of cells ","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 5","pages":"525-533"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}