中华劳动卫生职业病杂志最新文献

{"title":"[Predictive risk analysis for pneumoconiosis combined with tuberculosis].","authors":"M T Liu, Z Y B Fang, H L Zhao, Z Y Shi, R Hai, L Ning","doi":"10.3760/cma.j.cn121094-20240111-00013","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240111-00013","url":null,"abstract":"<p><p><b>Objective:</b> To explore the risk factors of pneumoconiosis complicated with pulmonary tuberculosis, to construct a clinical prediction model for patients with pneumoconiosis complicated with pulmonary tuberculosis, and to provide a scientific basis for the prevention of pneumoconiosis complicated with pulmonary tuberculosis. <b>Methods:</b> In January 2024, a total of 232 patients with pneumoconiosis (including coal workers' pneumoconiosis and silicosis) who were treated in the Department of Respiratory and Critical Care Medicine of the Third People's Hospital of Xinjiang Uygur Autonomous Region (Xinjiang Uygur Autonomous Region Occupational Disease Hospital) from January 2022 to January 2023 were randomly selected as the study subjects. Collectted basic patient information and diagnostic data. Multivariate logistic regression analysis was used to screen the risk factors related to pneumoconiosis complicated with pulmonary tuberculosis. According to the results of multivariate logistic regression analysis, a nomogram was established, and the area under the receiver operating characteristic (ROC) curve (AUC), calibration curve and decision curve analysis (DCA) were used to evaluate the predictive ability. <b>Results:</b> Among the 232 patients with pneumoconiosis, 73 were complicated with pulmonary tuberculosis, accounting for 31.47% (73/232). Multivariate logistic regression analysis determined that dust exposure time, type of work, smoking history, and lung function level were all risk factors for pneumoconiosis complicated with tuberculosis (<i>OR</i>=10.33, 95%<i>CI</i>=1.92~55.66, <i>OR</i>=5.43, 95% <i>CI</i>=1.91~15.44, <i>OR</i>=3.10, 95% <i>CI</i>=1.15~8.37, <i>OR</i>=4.00, 95% <i>CI</i>=1.62~9.87; <i>P</i><0.05). The constructed nomogram model has good clinical applicability when the area under the receiver operating characteristic (ROC) curve is 0.77 [95% <i>CI</i> (0.69, 0.73) ], the calibration curve is close to the ideal diagonal, the absolute error between the simulation curve and the actual curve is 0.03, and the DCA decision curve shows that the probability threshold of the nomogram model is 1%-90%. <b>Conclusion:</b> The risk of pneumoconiosis complicated with tuberculosis is high, and the risk factors of dust exposure time, smoking history, type of work and lung function level are high. This nomogram model can be used to predict the risk of pulmonary tuberculosis in patients with pneumoconiosis, which is helpful for early intervention.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"49-54"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The role of ferroptosis in renal injury induced by diquat].","authors":"M F Sun, L Zhu, X Chen","doi":"10.3760/cma.j.cn121094-20240117-00024","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240117-00024","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the role of ferroptosis in renal cell injury induced by diquat (DQ) . &lt;b&gt;Methods:&lt;/b&gt; From January to October 2022, human renal tubular epithelial (HK-2) cells were treated with DQ for 48 h, and different doses of ferroptosis inhibitors [deferoxamine (DFO), Fer-1] were added, and cells were harvested 24 h later. The experiment was divided into 6 groups (&lt;i&gt;n&lt;/i&gt;=6) : control group, DQ group (60 μmol/L), 20 μmol/L DFO (DFO-H) group, 10 μmol/L DFO (DFO-L) group, 5 μmol/L Fer-1 (Fer-1-H) group, 0.5 μmol/L Fer-1 (Fer-1-L) group. From December 2022 to June 2023, male C57bl/6 mice were selected to establish the animal model, and the experimental group was divided into 4 groups (&lt;i&gt;n&lt;/i&gt;=6) : control group, DQ group (25 mg/kg), DFO group (100 mg/kg) and Fer-1 group (2.5 μmol/kg). The changes of renal tissue were detected by HE staining. The fluorescence probe of ferrous ions was used to detect the change of iron ions in cells, and the colorimetric determination of total iron and ferrous ions in mouse kidney tissues was performed. Reverse transcription real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect mRNA and protein expression changes related to ferroptosis signaling pathway. TUNEL staining was used to detect apoptosis. Enzyme-linked immunosorbent assay (ELISA) was used to detect the changes of antioxidant-related proteins and oxidative stress-related products. Differences among groups were analyzed by one-way analysis of variance. &lt;b&gt;Results:&lt;/b&gt; In vitro test, compared with the control group, the iron ion level of HK-2 cells in DQ group was increased, the mRNA and protein expression levels of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and ferritin heavy chain (FTH) were decreased, the mRNA and protein expression levels of transferrin receptor 1 (TFR1) and divalent metal transporter 1 (DMT1) were increased, and the apoptosis level was significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The expression levels of glutathione (GSH) and super oxide dismutase (SOD) in HK-2 cells in DQ group were significantly lower than those in control group (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and the expression levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were significantly higher than those in control group (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with DQ group, iron ion levels in HK-2 cells in the intervention groups of DFO and Fer-1 at different doses were decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.001), and GPX4, SLC7A11 and FTH mRNA and protein expression levels were increased, and the mRNA and protein expression levels of TFR1 and DMT1 were decreased in DFO-H and DFO-L groups (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The apoptosis levels in the intervention groups of DFO and Fer-1 at different doses were decreased compared with DQ group (&lt;i&gt;P&lt;/i&gt;&lt;0.001), the expression levels of GSH and SOD were higher than those in DQ group (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and the expression levels of ROS were lower than those in DQ group (&lt;i&gt;P&lt;","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"14-24"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effects and mechanisms of PAD2 inhibitor AFM-30a attenuates pulmonary fibrosis in silicotic mice].","authors":"Y M Zhang, F Y Jin, X M Gao, H Xu, Y Zhu, N Mao","doi":"10.3760/cma.j.cn121094-20240102-00001","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240102-00001","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To observe the effects of peptidylarginine deiminase 2 (PAD2) inhibitor AFM-30a on silicotic mice and its possible mechanisms. &lt;b&gt;Methods:&lt;/b&gt; In May 2022, 40 SPF male C57BL/6J mice were randomly divided into control group, AFM-30a group, silicosis model group and AFM-30a treatment group, with 10 mice in each group. Silicosis model group and AFM-30a treatment group were perfused with silicon dioxide (SiO(2)) suspension (10 mg/piece, 50 μl), and the other groups were perfused with an equal amount of sodium chloride solution. After 2 weeks, AFM-30a group and AFM-30a treatment group were intraperitoneally injected AFM-30a (20 mg/kg, 100 μl) daily, and mice of other groups were injected with equal amounts of sodium chloride solution for 4 weeks. Mouse RAW264.7 monocytes/macrophages were cultured in vitro and divided into blank control group, AFM-30a group (5 μmol/L), SiO(2) group (200 μg/ml), and SiO(2)+AFM-30a group (200 μg/ml SiO(2) induction for 12 h, followed by 5 μmol/L AFM-30a treatment for 12 h). As well as blank control group, vimentin (Vim) group (2 μg/ml), citrullinated vimentin (Cit-Vim) group (2 μg/ml), and Cit-Vim+TLR4-C34 group (10 μmol/L TLR4-C34 treatment for 1 h, followed by 2 μg/ml Cit-Vim induction for 24 h). Hematoxylin Eosin (HE) and Masson staining were used to observe the pathological morphology of lung. The lung fieldclarity and lung texture of each group was observed by micro-CT. The number of positive cells was detected by tartrate resistant acid phosphatase (TRAP) staining. The localization and expression levels of PAD2, Cit-Vim, toll-like receptor 4 (TLR4) signaling and receptor activator of nuclear factor-κB ligand (RANKL) signaling proteins were measured by Immunofluorescence staining and Western blotting in vitro and in vivo. The experimental data were all presented as Mean±SD. A completely random design of one-way analysis of variance was used among the groups. The pduo comparison was performed using LSD test for homogeneity of variance and Tamhane's test for inconsistency. &lt;b&gt;Results:&lt;/b&gt; Compared with the control group, the silicosis model group showed the formation of silicon nodules accompanied by collagen deposition, the silicosis model group showed thickened, and several high-density shadows of varying sizes in the lung field, and the number of TRAP positive cells in silicosis model group were increased significantly, the expression levels of PAD2, Cit-Vim, TLR4 and RANKL signal-related proteins were also significantly increased in silicosis groupmodel (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with the silicosis model group, the AFM-30a treatment group reduced deposition of collagen in lung, and the number of TRAP positive cells was decreased in AFM-30a treatment group. The expression levels of PAD2, Cit-Vim, TLR4 and RANKL signaling related proteins were significantly decreased in AFM-30a treatment group (&lt;i&gt;P&lt;/i&gt;&lt;0.05). In vitro, compared with the blank control group, the number of TRAP positive cells a","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of occupational lead exposure on the indicators of immune function of workers].","authors":"Z X Li, Y Li, L Li, T Hou, X H Wu, Y H Chen, B Chen, Z L Shang","doi":"10.3760/cma.j.cn121094-20240428-00191","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240428-00191","url":null,"abstract":"<p><p><b>Objective:</b> To investigatethe effects of different blood lead levels on indicators of immune function in occupationally lead-exposed populations. <b>Methods:</b> From October to December 2023, a total of 126 occupationally exposed lead workers of a company in Guizhou Province were selected, and their basic information was collected through questionnaires. Inductively coupled plasma mass spectrometry was used to detect blood lead levels in the study population. Workers were categorized into Group 1, Group 2 and Group 3 based on blood lead levels (blood lead levels <200 μg/L, 200~400 μg/L and >400 μg/L). Lymphocyte subpopulation marker leukocyte differentiation antigen (CD) in peripheral blood and interleukin (IL) -1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17, tumor necrosis factor-alpha (TNF-α), and interferon (IFN) -γ in serum were examined by flow cytometry. Serum levels of immunoglobulin (Ig) A, IgG, IgM, and complement proteins (C3, C4) were measured by immunoscattering turbidimetry. Data were statistically analyzed using rank sum test, Chi-square test or Fisher exact probability method for multiple samples. <b>Results:</b> Compared with group 1, the percentage of CD3(+)CD8(+) and CD4(+)CD25(+) cells decreased (<i>P</i><0.05) and the percentage of CD4(+) CD95(+) cells increased in the lead-exposed populations in groups 2 and 3 (<i>P</i><0.05) ; however, the serum IL-1β, IL-2, IL- 5, IL-8, IL-12p70, and IFN-γ levels were decreased (<i>P</i><0.05) in group 3. Meanwhile, IgG (<i>P</i><0.05) and IgM levels in serum of lead-exposed population in group 2 and group 3 were reduced (<i>P</i><0.05) comparing with group 1. Spearman correlation analysis showed that blood lead levels of workers were negatively correlated with the percentage of peripheral blood CD8(+)CD95(+) cells, CD3(+)CD8(+) cells, CD3(-)CD16(+) CD56(+) cells, IgG, IgM, and IgA (<i>r</i>(s)=-0.20, -0.22, -0.23, -0.24, -0.26, -0.35, <i>P</i><0.05), but was positively correlated with the level of CD3(+)CD4(+) cells (<i>r</i>(s)= 0.18, <i>P</i><0.05). Regression analysis revealed that blood lead level was a risk factor for the percentage of CD8(+)CD95(+) cells, CD3(+)CD8(+) cells, CD3(-)CD16(+) CD56(+) cells, IgG, IgM, and IgA (<i>P</i><0.05) . <b>Conclusion:</b> Different lead loads can lead to abnormal changes in peripheral blood lymphocyte subsets, cytokines, and immunoglobulin levels in occupationally lead-exposed people. Lead exposure in occupationally lead-exposed populations may affect their immune function.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"37-43"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on the current situation and influencing factors of uncivilized behavior in workplace encountered by nurses in Xianyang City].","authors":"L Wang, H Fan, X J Wang, X X Xie, Z F Liu","doi":"10.3760/cma.j.cn121094-20231114-00113","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231114-00113","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the current situation of uncivilized behavior in workplace encountered by nurses in Xianyang City, and analyze its influencing factors, so as to provide a basis for formulating corresponding intervention measures. <b>Methods:</b> In May 2023, convenience sampling was used to investigate the general information, current situation of uncivilized behavior in workplace and coping style of 1310 nurses in 8 secondary and tertiary general hospitals in Xianyang City by using the general information questionnaire, the Chinese version of the Nurse Incivility Scale and the Simplified Coping Style Questionnaire. Multiple linear regression analysis was used to analyze the influencing factors of uncivilized behavior encountered by nurses. <b>Results:</b> A total of 1285 valid questionnaires were collected, with an effective recovery rate of 98.09%. The total score of uncivilized behavior encountered by nurses was (86.41±22.49) points. Among them, the scores of five dimensions were nurses' uncivilized behavior (19.95±4.70) points, patients' uncivilized behavior (23.66±8.96) points, supervisors' uncivilized behavior (13.25±4.87) points, doctors' uncivilized behavior (14.32±5.63) points and colleagues' uncivilized behavior (15.23±4.93) points. The multiple linear regression analysis results showed that the hospital level and coping style were the significant influencing factors of uncivilized behavior in workplace encountered by nurses (<i>β</i>=0.104, 0.056; <i>P</i><0.05) . <b>Conclusion:</b> The current situation of uncivilized behavior in workplace encountered by nurses needs to be improved. Hospital managers should focus on coping styles, such as setting up simulation scene exercises, team cooperation training, training and counseling, so as to reduce the uncivilized behavior in workplace encountered by nurses.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"58-61"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of serum tumor marker levels in 629 patients with pneumoconiosis].","authors":"Y Li, G Xu, S C Mao, H Y Lu, L Han","doi":"10.3760/cma.j.cn121094-20240422-00175","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240422-00175","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the relationship between serum tumor markers and the severity of silicosis. <b>Methods:</b> In April 2024, A cluster sampling was conducted on the pulmonary rehabilitation centers in Jiangsu Province. A retrospective analysis was carried out on 629 patients who received follow-up physical examinations for pneumoconiosis from April to November 2023 at the selected rehabilitation centers. 281 healthy volunteers who underwent occupational health examinations at our institution were selected as the blank control group, and 384 dust exposed workers from a coal mining enterprise were selected as the dust exposed control group. Detect the levels of peripheral bloodtumor markers carcinoembryonic antigen CEA, carbohydrate antigen CA199, and neuron-specific enolase NSE in specimens. Measure the patient's forced vital capacity (FVC), forced expiratory volume in one second (FEV(1)), and lactate acid dehydrogenase (LDH) levels in the blood. The Jonckheere-Terpstra test, a non-parametric test for ordered independent samples, was used to compare the expression levels of tumor markers among multiple groups. Spearman's rank correlation analysis was adopted to explore the correlations between serum tumor markers and the staging of pneumoconiosis as well as lung function parameters. <b>Results:</b> Among the 629 patients with pneumoconiosis, 454 were in stage Ⅰ, 150 were in stage Ⅱ, 25 were in stage Ⅲ. There were significant differences in serum CEA, CA199 and NSE levels among patients with different periods of pneumoconiosis, blank control group and dust-exposed control group (<i>P</i><0.001). Serum CEA, CA199 and NSE levels were positively correlated with pneumoconiosis stage (<i>P</i><0.05). There is a negative correlation between serum CA199 levels and FEV(1)% in patients with pneumoconiosis (<i>r</i>=-0.082, <i>P</i><0.05). The serum CA199 level was positively correlated with LDH in patients with pneumoconiosis (<i>r</i>=0.108, <i>P</i><0.05) .The serum NSE level of pneumoconiosis patients was negatively correlated with FVC% (<i>r</i>=-0.079, <i>P</i><0.05) .There is a positive correlation between serum NSE levels and LDH in patients with pneumoconiosis (<i>r</i>=0.435, <i>P</i><0.05) . <b>Conclusion:</b> The serum CA199 and NSE of pneumoconiosis patients are directly related to the severity and stage of the disease, which have practical significance in judging the treatment effect of pneumoconiosis patients and monitoring the development of the disease.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"54-57"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Influencing factors and risk analysis of anxiety and depression in occupational population].","authors":"Y L Chen, J X Ma, Y J Lan, N Li, H J Wang, L M Quan","doi":"10.3760/cma.j.cn121094-20240315-00100","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240315-00100","url":null,"abstract":"<p><p><b>Objective:</b> To identify and evaluate the important risk factor set of anxiety and depression in occupational population, establish a risk prediction model, and provide scientific basis for making targeted mental health protection plan and promoting the mental health of workers. <b>Methods:</b> In August 2016, a cluster random sampling method was used to investigate 807 employees who underwent physical examination in a hospital as research objects. The simplified Chinese version of the core job content questionnaire, Athens Insomnia Scale, AIS-5 and Symptom Check List-90 (SCL-90) were used for the Occupational stress, insomnai and negative emotional symptom investigation. Chi-square and Fisher exact probability method were used for data analysis, and Bayesian network was used for model construcion and analysis. <b>Results:</b> The score of occupational stress was 0.88±0.15, and the incidence of occupational stress was 18.09% (146/807). AIS-5 scores were (3.03±2.82), and the incidence of insomnia was 15.99% (129/807). Depression (16.89±5.73) scores, anxiety (12.36±4.11) scores. Depression (16.89±5.73) score, anxiety (12.36±4.11) score, the detection rate was 8.55% (69/755), 7.31% (59/762). Gender, illness, education, insomnia and occupational stress were correlated with depression (<i>P</i><0.01), while education, illness, insomnia and anxiety were correlated (<i>P</i><0.05). When both occupational stress and insomnia existed, the detection rate of depression was the highest (0.4006) . <b>Conclusion:</b> Insomnia was a valid predictor of anxiety and depression, suggesting that occupational groups should pay attention to sleep quality and managers should rationalize work tasks in order to reduce the risk of anxiety and depression.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"44-48"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Experimental study on early sensitive indexes of acute kidney injury in rats poisoned by diquat].","authors":"L J Yu, Z C Zhang, Y Z Wu, W J Wang, X D Jian, B T Kan","doi":"10.3760/cma.j.cn121094-20231221-00164","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231221-00164","url":null,"abstract":"<p><p><b>Objective:</b> To establish the model of acute kidney injury (AKI), search for more sensitive and reliable biomarkers. <b>Methods:</b> In April 2018, 100 male Wister rats aged 6 to 8 weeks were selected and randomly divided into experimental group (n=90) and control group (n=10). The experimental group was given Diachalefin (140 mg/kg body weight) by intragastric administration, while the control group was given saline intragastric administration. Ten rats in the experimental group were killed 0.5 h, 2 h, 6 h, 24 h, 3 d, 7 d, 14 d, 21 d and 28 d after intragastric administration, respectively. Serum creatinine (Cr), urea nitrogen (BUN) and uric acid (UA) were detected by automatic biochemical analyzer with 5 ml of blood from inferior vena cava puncture. Serum neutrophil gelatinase-associated lipid carrier protein (NGAL), kidney damage molecule-1 (KIM-1) and transforming growth factor-β1 (TGF-β1) levels were determined by enzyme-linked immunosorbent assay (ELISA). The data between groups were compared using two independent sample t tests. <b>Results:</b> The renal tissue structure of rats in the control group was not significantly abnormal, while the renal tissue cell damage of rats in the experimental group was obvious, which gradually increased with the extension of time in the early stage, and gradually recovered in the later stage. UA in experimental group reached its peak at 24 h after exposure and was still higher than that in control group at 14 d (<i>P</i><0.05), Cr reached its peak at 7 d, and then gradually decreased, and there was no statistical significance between experimental group and control group at 28 d (<i>P</i>>0.05). BUN increased at 6 h after exposure and reached the highest value at 7~14 d (<i>P</i><0.05). Blood NGAL increased at 0.5 h after exposure, reached its peak at 24 h, continued to increase at 3, 7 and 14 days (<i>P</i><0.05), and began to decrease at 21 days. KIM-1 began to increase at 0.5 h, continued to peak at 24 h, 3 and 7 d after exposure, and began to decrease at 14 d, but it was still higher than that in control group (<i>P</i><0.05). There was no significant difference in TGF-β1 at each time point (<i>P</i>>0.05). Western blot assay results: Compared with control group, there was no significant difference in the expression level of TGF-β1 in kidney tissue of experimental group (<i>P</i>>0.05). NGAL increased gradually from 2 h and was higher at 7 and 14 d, with statistical significance (<i>P</i><0.05). KIM-1 increased at 2 h, decreased at 6 and 24 h, and increased again at 3 and 7 d. <b>Conclusion:</b> NGAL and KIM-1 can be used as early diagnostic biomarkers for diquat-induced acute kidney injury.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"62-67"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A cross-sectional study of the characteristics of patients with pneumoconiosis complicated with chronic obstructive pulmonary disease].","authors":"Y Chen, P P Song, Y N Wei, L Y Tian, H Zhang, Y J Yan","doi":"10.3760/cma.j.cn121094-20231011-00083","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231011-00083","url":null,"abstract":"<p><p><b>Objective:</b> To analyze the characteristics of patients with pneumoconiosis complicated with chronic obstructive pulmonary disease (COPD), and to explore the comorbidity of pneumoconiosis and COPD and its influencing factors. <b>Methods:</b> From October to December 2022, 255 pneumoconiosis patients admitted to an occupational disease prevention and control hospital from January 2018 to December 2021 were selected as the study subjects. According to whether the pneumoconiosis patients were complicated with COPD or not, they were divided into pneumoconiosis and COPD comorbidity group and pneumoconiosis group. The general condition and dust exposure of the two groups of patients were analyzed, and the relationship between different types and different periods of pneumoconiosis and COPD comorbidity was analyzed by multivariate logistic regression. <b>Results:</b> A total of 255 subjects were collected, including 64 patients with comorbidity of pneumoconiosis and COPD, and the comorbidity rate was 25.1%. There were 186 males (72.9%) and 69 females (27.1%), ranging in age from 35 to 90 (63.79±11.79) years, and working age from 1 to 45 (20.31±10.57) years. The comorbidity of pneumoconiosis and COPD increased with the increase of working age (χ(2) (trend)=8.19, <i>P</i>=0.004), and the comorbidity rate for COPD with working age of more than 30 years was 37.7% (23/61). The comorbidity rate of pneumoconiosis and COPD also increased with the increase of the stage of pneumoconiosis (χ(2)(trend)=13.14, <i>P</i><0.001), and the comorbidity rate of pneumoconiosis and COPD in the stage Ⅲ was as high as 44.0% (11/25). The cumulative dust exposure was negatively correlated with forced expiratory volume in one second/forced vital capacity (FEV(1)/FVC), and the linear regression equation <i>y</i>=-0.04<i>x</i>+78.4. Multivariate logistic regression analysis showed that the length of services ≥30 years (<i>OR</i>=3.30, 95%<i>CI</i>: 1.15-9.52) and stageⅡ (<i>OR</i>=3.05, 95%<i>CI</i>: 1.03-9.04) were the risk factors for comorbidity between pneumoconiosis and COPD (<i>P</i><0.05) . <b>Conclusion:</b> The comorbidity rate of pneumoconiosis and COPD is high. Working age, pneumoconiosis stage and cumulative dust exposure are the main influencing factors of pneumoconiosis and COPD comorbidity, so more attention should be paid to the comorbidity of pneumoconiosis and COPD.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"32-37"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The interaction between plasma aluminum concentration and schooling year on cognitive function].","authors":"L M Ma, Y J Xue, X Y Li, S S Wang, J S Zhang, Q Niu","doi":"10.3760/cma.j.cn121094-20240104-00003","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240104-00003","url":null,"abstract":"<p><p><b>Objective:</b> To explore the effect of the interaction between plasma aluminum concentration and schooling year on cognitive function, it provides the basis for early identification of its damaging effect. <b>Methods:</b> From October to December, 2014, 1114 on-the-job aluminum exposed workers in a large aluminum factory in Shanxi Province were investigated. The plasma aluminum concentrations were measured by inductively coupled plasma-mass spectrometry (ICP-MS) as an internal exposure indicator. The subjects were divided into low, medium, and high aluminum exposure group based on the blood lead concentration levels. The general information was collected with a self-designed questionnaire, and their cognitive function was evaluated with the mini-mental state examination and the clock drawing test. The interaction between plasma aluminum concentration level and education level was analyzed by using unconditional logistic regression fitting multiplication model and cross analysis fitting addition model. <b>Results:</b> The average level of plasma aluminum of the subjects was 21.68 (10.51, 45.78) μg/L. The detection rate of cognitive impairment was 19.9%. The influencing factors of cognitive function were plasma aluminum concentration, age, education level and marital status, among which high schooling year, and being married were the protective factors (all <i>P</i><0.05), plasma aluminum concentration, and age were the risk factors (all <i>P</i><0.05) ; After adjusting the confounding factors, such as age, marital status, schooling year, income level, smoking, and drinking, logistic regression model and the multiple linear regression model showed that there was a dose response relationship between plasma aluminum concentration and cognitive impairment. With the increase of plasma aluminum concentration, the score gradually decreased, and the risk of cognitive impairment increased (<i>P</i>(trend)<0.05) ; There were multiplicative interaction (<i>AOR</i>=2.15, 95%<i>CI</i>: 1.10-4.19) and additive interaction (<i>RERI</i>=5.38, 95%<i>CI</i>: 2.60-8.16; <i>AP</i>=0.65, 95% <i>CI</i>: 0.51-0.80; <i>S</i>=3.88, 95% <i>CI</i>: 2.00-7.56) between plasma aluminum concentration and low schooling year on cognitive function. <b>Conclusion:</b> There was a dose-response relationship between aluminum exposure and cognitive impairment. There was an interaction between aluminum exposure and low schooling year. When both of them worked together, the risk of cognitive impairment increases.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"25-31"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}