中华劳动卫生职业病杂志最新文献

{"title":"[A case of rare pathological type of lung cancer caused by occupational exposure to asbestos].","authors":"M Y Qiu, J J Zhang, P P Song, T Luan, L M Luo, L Qin, X W Sun","doi":"10.3760/cma.j.cn121094-20241030-00495","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20241030-00495","url":null,"abstract":"<p><p>Asbestos is a risk factor for occupational lung cancer. This article reported a case of asbestosis. During regular chest CT scans, an enlargement of nodules in the left lower lobe was observed. After undergoing relevant examinations, the patient underwent left lower lobe resection. Postoperative pathology confirmed the diagnosis as SMARCA4-deficient undifferentiated tumor. The patient was subsequently treated with immunotherapy, radiotherapy and chemotherapy and currently has a good prognosis.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"314-317"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Investigation and analysis of CT dose in adult examinees in Tianjin City].","authors":"Z Niu, S L Yang, Y Ni, C X Liu, Y Liu, J M Zhang","doi":"10.3760/cma.j.cn121094-20241213-00564","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20241213-00564","url":null,"abstract":"<p><p><b>Objective:</b> To understand the radiation dose levels and distribution characteristics received by adult examinees during computed tomography (CT) examinations in Tianjin City, so as to provide scientific references for further optimizing CT examination protocols and reducing unnecessary radiation exposure. <b>Methods:</b> From October 2022 to September 2023, a stratified quota sampling method was used to select 15 medical institutions. One CT device was chosen from each institution, and a total of 1803 adult examinees undergoing CT examinations were investigated. Information including scanning region, tube voltage, tube current-time product, scanning length, volume computed tomography dose index (<i>CTDI</i>(vol)), and dose-length product (<i>DLP</i>) were collected and analyzed. The Kruskal-Wallis <i>H</i> test was applied to compare dose parameters among different grades of medical institutions and different scanning regions, with the Bonferroni method used to adjust the significance level for pairwise post hoc comparisons. The Cochran-Armitage trend test was used to analyze the dose trend across different numbers of detector rows. <b>Results:</b> In this survey, the median ages (M) of examinees undergoing cranial, chest, abdominal, and lumbar spine CT examinations were 42, 40, 41, and 43 years old, respectively. The median tube voltages were all 120 kV. The median tube current-time products were 268, 140, 196, and 242 mAs. The 75(th) percentiles (<i>Q</i>(3)) of <i>CTDI</i>(vol) were 60, 14, 20, and 23 mGy, and the <i>Q</i>(3) values of <i>DLP</i> were 844, 480, 758, and 612 mGy·cm. With the exception of abdominal <i>CTDI</i>(vol), <i>CTDI</i>(v)ol and <i>DLP</i> showed a decreasing trend with an increase in the number of CT detector rows, and the differences were statistically significant (<i>P</i><0.05) . <b>Conclusion:</b> The <i>Q</i>(3) values of <i>CTDI</i>(vol) and <i>DLP</i> for adult CT examinations in Tianjin City were comparable to the national diagnostic reference levels. The <i>Q</i>(3) value of <i>CTDI</i>(vol) ranked at a moderate level among provinces and municipalities, while the <i>Q</i>(3) value of <i>DLP</i> remained at a relatively high level. Therefore, in clinical practice, it is advisable to minimize the scanning field of examinees to reduce radiation dose exposure and safeguard their health and safety.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"288-292"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparative study on occupational exposure limits of chemical hazardous factors in China and NIOSH limits in the United States].","authors":"X H Qian, J Dang, X Liu, L Han, B L Zhu","doi":"10.3760/cma.j.cn121094-20250828-00342","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20250828-00342","url":null,"abstract":"<p><p><b>Objective:</b> To compare and analyze the occupational exposure limits (OELs) specified in China's \"Occupational Exposure Limits for Hazardous Agents in the Workplace-Part 1: Chemical Hazardous Agents\" (GBZ 2.1-2019) with the recommended exposure limits (RELs) issued by the National Institute for Occupational Safety and Health (NIOSH) in the United States, aiming to identify chemical hazardous factors requiring optimization of OELs. <b>Methods:</b> In March 2025, using the Chemical Abstracts Service registration number (CAS RN) as the core matching identifier, OELs from GBZ 2.1-2019 and NIOSH RELs in the United States were entered into Excel 2019 to construct a database. The OELs were matched one-to-one according to their classification types. Descriptive statistical methods were employed to analyze the ratio of exposure limits, and content comparisons were conducted to evaluate differences in classification types, legal status, and labeling applications. <b>Results:</b> The types and connotations of limits in China and the United States were basically the same. Among the 272 shared limits, 38.2% (104 items) were consistent, 23.9% (65 items) were basically consistent, and 37.9% (103 items) showed significant differences. There were 51 China standards that were significantly stricter than NIOSH standards in the United States, and 17 China standards that were significantly more lenient than NIOSH standards in the United States. Differences existed between China and the United States limits in terms of legal status and the application of certain labels. <b>Conclusion:</b> Domestic interim standards should be formulated as needed for internationally recognized but unestablished OELs, and OELs that are excessively strict, overly broad or inconsistent with type values should be adjusted. It is recommended to shorten or establish a dynamic OELs update mechanism.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"304-308"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Qualitative comparative analysis of high-risk configurations of occupational stress, burnout and insomnia among primary medical and health personnel in a certain area of Hebei Province].","authors":"J Q Zhao, L X Yang, M Du, G L Yin, E B Wang, J G Li","doi":"10.3760/cma.j.cn121094-20240902-00409","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240902-00409","url":null,"abstract":"<p><p><b>Objective:</b> To understand the current situation of occupational stress, burnout, and insomnia among primary medical and health personnel in a certain area of Hebei Province, and the qualitative comparative analysis (QCA) was used to frame the high-risk population configuration for occupational stress, burnout, and insomnia among primary medical and health personnel, providing a basis for precise intervention. <b>Methods:</b> In August 2021, all employees of 7 public hospitals and 8 public health institutions in Xiong'an New Area were selected as survey objects. Questionnaire survey was conducted using the general situation questionnaire, Effort Reward Imbalance Scale (ERI), Maslach Burnout Inventory-General Survey (MBI-GS), and the Ashens Insomnia Scale (AIS). The chi-square test was used for univariate analysis, and the meaningful variables in the univariate analysis were included in the QCA to analyze the configuration of high-risk factors of occupational stress, burnout and insomnia among primary medical and health personnel. <b>Results:</b> 2675 valid questionnaires were collected, with an effective response rate of 88.63% (2675/3018). The overall incidence of occupational stress among primary medical and health personnel was 51.48% (1377/2675), including 58.53% for doctors, 59.54% for nurses, and 31.14% for public health personnel. The incidence of burnout was 57.12% (1528/2675), including 59.00% for doctors, 63.95% for nurses, and 49.14% for public health personnel. The incidence of insomnia was 56.75% (1518/2675), including 59.72% for doctors, 62.07% for nurses, and 38.86% for public health personnel. Not drinking alcohol or occasionally drinking alcohol was a necessary condition for primary medical and health personnel to experience occupational stress, the consistency value (<i>C</i>) was 0.951. The configuration analysis results showed that the high-risk factors configurations of occupational stress were mainly manifested as high effort type and low return and no exercise type. The high-risk factor configurations for burnout were low personal achievement type and high occupational stress type, while the high-risk factor configurations for insomnia were high economic burden and high internal investment type and high occupational stress type. The overall consistencies of the configurations were 0.864, 0.828 and 0.914, respectively. <b>Conclusion:</b> The occupational stress, burnout, and insomnia of primary medical and health personnel in Hebei Province cannot be ignored, and their occurrence is the result of multiple influencing factors. The QCA method can frame the configuration of high-risk factors and provide a basis for precise intervention.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"267-273"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Quantitative analysis of policy texts on occupational health technical service institutions in China based on policy tools].","authors":"X Y Zhang, T Liu, F Han","doi":"10.3760/cma.j.cn121094-20250318-00102","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20250318-00102","url":null,"abstract":"<p><p><b>Objective:</b> To analyze the current status and structural characteristics of policy development concerning occupational health technical service institutions in China, as well as providing an empirical basis and decision-making assistance for subsequent policy optimization in this field. <b>Methods:</b> In December 2024, the relevant policy documents were searched on the government's official website with the keyword \"occupational health technical services\" from 1999 to 2024, the included policy texts were subjected to standardized cleaning, and a \"policy instruments-target groups-policy effectiveness\" three-dimensional analytical framework was constructed, content analysis was adopted for coding, and both single-dimensional descriptive analysis and multi-dimensional cross-analysis were conducted. <b>Results:</b> A total of 44 valid policy documents were ultimately included, yielding 247 standardized policy items. Among these, there were 137 demand-oriented policy tools (accounting for 55.47%), 73 environmental-oriented policy tools (accounting for 29.55%), and 37 supply-oriented policy tools (accounting for 14.98%). The policy items primarily targeted occupational health technical service institutions and health administrative departments at various levels, while fewer items addressed disease prevention and control centers, professional technicians, and previously accredited entities. <b>Conclusion:</b> Current policies for occupational health technical service institutions in China exhibit structural imbalances in policy tool utilization and insufficient coverage of core supporting entities and critical operational aspects. Recommendations include optimizing policy tool structures based on local conditions and enhancing the synergistic application of supply-oriented and environmental-oriented policy tools.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"299-303"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Five cases of acute poisoning caused by inhalation of acrolein gas].","authors":"Y T Zeng, Y Y He, D X Sun","doi":"10.3760/cma.j.cn121094-20241113-00522","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20241113-00522","url":null,"abstract":"<p><p>Acrolein is a highly toxic chemical substance commonly found in industrial production. This article analyzes the clinical data of 5 patients suspected of acute acrolein poisoning. The patients presented symptoms such as bilateral bronchitis, cyanosis of the lips and face, pharyngalgia, chest tightness, shortness of breath, etc. Severe poisoning patients also experienced acute respiratory failure and acute respiratory distress syndrome (ARDS). After comprehensive treatments including high-flow humidified oxygen inhalation, corticosteroid pulse therapy, and blood purification, 4 of the moderate-to-severe poisoning patients gradually improved and were discharged from the hospital, while 1 severe poisoning patient significantly improved after treatment with extracorporeal membrane oxygenation (ECMO), but died later due to secondary infection. This article provides valuable experience for clinicians in identifying, diagnosing, and treating acrolein poisoning, and highlights the risk of secondary infection in severe patients during treatment, which is significant for improving the success rate of treatment.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"318-320"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The current status and influencing factors of occupational health literacy of workers in a nuclear industry enterprise].","authors":"K Zhu, X F Bai, Y Zhao, T Zhang, Y J Hao, L Y Guo, S Jiang, J M Zhan","doi":"10.3760/cma.j.cn121094-20241106-00505","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20241106-00505","url":null,"abstract":"<p><p><b>Objective:</b> To understand the occupational health literacy (OHL) level of workers in a nuclear industry enterprise, analyze its influencing factors, and provide a reference basis for conducting targeted occupational health training and improving the OHL level. <b>Methods:</b> In May 2024, a cluster sampling method was adopted, and 9169 workers from a nuclear industry enterprise were selected as the research subjects. The \"Personal Questionnaire for Occupational Health Literacy Monitoring Survey of Nuclear Industry Enterprise\" was used to investigate their demographic information and OHL level. Pearson <i>χ</i>(2) test was used to analyze the OHL level, and a binary logistic regression model was used to analyze the influencing factors. <b>Results:</b> Among the workers in the nuclear industry enterprise, there were 7101 males and 2068 females, with an average age of (43.4±9.6) years old. The overall OHL level was 42.7% (3912/9169), and the OHL levels of the four dimensions of occupational health legal knowledge, basic knowledge of occupational health protection, basic skills of occupational health protection, and healthy work styles and behaviors were 72.3% (6628/9169), 31.4% (2880/9169), 39.6% (3629/9169) and 76.6% (7021/9169), respectively. Logistic regression analysis results showed that age, education level, and average weekly working hours were the influencing factors of overall OHL level (<i>P</i><0.05). The overall OHL levels of workers aged 28-37, 38-47 and ≥ 48 were lower than those of workers aged 18-27 (<i>P</i><0.05), and the overall OHL levels of workers with a bachelor's degree or above and postgraduate education were higher than those with education below a bachelor's degree (<i>P</i><0.05), and the overall OHL levels of workers who worked an average of 41-44 hours per week were higher than those who worked ≤40 hours per week (<i>P</i><0.05) . <b>Conclusion:</b> The OHL level of workers in the nuclear industry enterprise needs to be further improved. Older age, lower education, and average weekly working hours ≤ 40 hours workers are the key groups for improving OHL level. Targeted OHL improvement measures should be formulated in the future.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"281-287"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The role and mechanism of S1PR2 inhibitor JTE-013 in attenuating pulmonary fibrosis in silicosis mice].","authors":"J Zhang, S Wu, Y H Chen, M Zou, Y Li","doi":"10.3760/cma.j.cn121094-20250529-00214","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20250529-00214","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the effects of the sphingosine-1-phosphate receptor-2 (S1PR2) inhibitor JTE-013 on pulmonary fibrosis in silicosis mice and its underlying molecular mechanisms. &lt;b&gt;Methods:&lt;/b&gt; In October 2024, 40 SPF male C57BL/6J mice were randomly divided into control group, JTE-013 control group, silicosis model group, and JTE-013 treatment group. A silicosis model was established by non-exposure intratracheal instillation of SiO(2) suspension. One week after model was establishment, mice in the JTE-013 treatment group were intraperitoneally injected with JTE-013 (10 mg/kg, twice a week for a total of 6 times), while mice in other groups were intraperitoneally injected with the same volume of normal saline. After 28 d of modeling, the lung coefficients of mice in each group were detected. The lung tissues were stained with HE, Masson, and Sirius Red to assess pathological damage and collagen deposition. The content of hydroxyproline (HYP) was determined. The expressions of S1PR2, epithelial-mesenchymal transition (EMT) marker proteins [E-cadherin (E-cad), Vimentin, α-smooth muscle actin (α-SMA) ], and Ras homolog family member A/Rho-related coiled helix kinase 1 (RhoA/ROCK1) pathway proteins were detected by protein immunoblotting method. Human alveolar epithelial A549 cells were cultured in vitro. The cells were divided into normal control group (cultured in complete medium for 26 h), JTE-013 control group (cultured in medium prepared to 1 μmol/L JTE-013 solution for 26 h), SiO(2) treatment group (cultured for 2 h with normal medium, then treated with 50 μg/ml SiO(2) suspension for 24 h), and SiO(2)+JTE-013 treatment group (pre-treated with 1 μmol/L JTE-013 for 2 h, then added 50 μg/ml SiO(2) suspension for 24 h). After group intervention, the expression levels of S1PR2, EMT-related proteins, RhoA and ROCK1 in the cells were detected. For the normally distributed measurement data, one-way ANOVA analysis of variance was used for inter-group comparison, and LSD-&lt;i&gt;t&lt;/i&gt; test was used for pairwise comparison. &lt;b&gt;Results:&lt;/b&gt; Compared with the control group, the mice in the silicosis model group had a significant decrease in body weight, and their lung coefficient and HYP content were significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05), silicotic nodules formed in the lungs, accompanied by collagen deposition. And the expression levels of SIPR2 protein, EMT-related proteins Vimentin, α-SMA, and the RhoA and ROCK1 proteins in signaling pathway were significantly increased, while the expression level of the epithelial marker E-cad was significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with the silicosis model group, the mice in the JTE-013 treatment group had a significantly increase in body weight, the lung coefficient and HYP content were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05), the pulmonary fibrosis was significantly reduced. And the expression levels of SIPR2 protein, EMT-related proteins Vimentin, α-SMA, and the RhoA and ROCK1 prote","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"247-257"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on the relationship between occupational stress and social support with job burnout of locomotive drivers].","authors":"G Q Liu, H L Huang, X R Huang, H F Guo, Q C Jia, S W Ma","doi":"10.3760/cma.j.cn121094-20240722-00332","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240722-00332","url":null,"abstract":"<p><p><b>Objective:</b> To explore the mediating role of anxiety and social support between occupational stress and job burnout among locomotive drivers, and the regulatory role of self-requirement. <b>Methods:</b> A total of 1993 front-line drivers from 5 rail transit enterprises were selected as subjects by cluster random sampling method from March to June 2022. The job burnout, occupational stress, anxiety, social support and self-requirement were investigated by using the Marquis Work Burnout Inventory (General Version), Job Stress Survey, Generalized Anxiety Scale, Self-Reported Health Rating Scale and Self-Examination Questionnaire. Bootstrap method was used to examine the mediating effect of anxiety and social support between occupational stress and job burnout, and the regulatory effect of self-requirement. <b>Results:</b> The comprehensive score of job burnout for locomotive drivers was 2.60 (1.95, 3.24) points. There were statistically significant differences in the job burnout scores among drivers with different total working years, current job years, monthly working hours, and smoking status (<i>H</i>=12.12, 22.76, 41.36, 18.20, <i>P</i><0.05). The mediating effect analysis showed that the partial mediating effect composed of occupational stress-anxiety-job burnout accounted for 30.8% of the total effect (<i>β</i>=0.008, 95%<i>CI</i>: 0.007-0.009), and the partial mediating effect composed of occupational stress-social support-job burnout accounted for 11.5% of the total effect (<i>β</i>=0.003, 95%<i>CI</i>: 0.003-0.004). The analysis of moderated mediating effect showed that the increase of self-requirement could amplify the proportion of the partial mediating effect of anxiety in the total effect (from 21.4% to 44.0%), and reduce the direct effect of work stress on job burnout and the partial mediating effect of social support. <b>Conclusion:</b> The occupational stress of locomotive drivers can indirectly affect job burnout through anxiety and social support. It is suggested to identify the self-requirement level of locomotive drivers and carry out targeted intervention on anxiety and social support to slow down the formation of job burnout.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"293-298"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effect of the PI3K/Akt/HIF-1α signaling pathway in the changes of urinary metabolite in silicosis mice model].","authors":"X Tan, B Dong, Y Z Lu, Y Q Fu, Y D Li, F H Shen, W H Zhang","doi":"10.3760/cma.j.cn121094-20250328-00119","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20250328-00119","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To explore the regulatory effect of the phosphatidylinositol 3-kinase (PI3K) /protein kinase B (AKT) /hypoxia-inducible factor 1α (HIF-1α) glycolysis signaling pathway on urinary metabolites during the progression of silicosis fibrosis and its related metabolic pathway mechanisms. &lt;b&gt;Methods:&lt;/b&gt; From March to June 2023, 36 SPF-grade C57BL/6 mice were randomly divided into the control group, the silica (SiO(2)) model group, the PI3K inhibitor LY294002 early/late intervention group, and the HIF-1α inhibitor 2-methoxyestradiol (2-ME2) early/late intervention group, totaling 6 groups, with 6 mice in each group. The silicosis mice model was established by tracheal instillation 50 μl of 200 mg/ml SiO(2) suspension. Corresponding intervention agents were intraperitoneally injected on the 2nd day (early stage) and on the 29th day (late stage) after dust exposure, and the intervention was carried out continuously for 55 d and 28 d respectively. On the 56th day of the experiment, the mice were sacrificed and the lung tissues were subjected to pathological examination. Non-targeted metabolomics analysis of urine was conducted using ultra-performance liquid chromatography-tandem mass spectrometry. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to screen out the differential metabolites, and metabolic pathway enrichment analysis was performed. &lt;b&gt;Results:&lt;/b&gt; Mice in the SiO(2) model group exhibited dull fur coloration, reduced activity, diffuse pulmonary fibrosis, alveolar structural damage, and extensive inflammatory cell infiltration after dust exposure. All intervention groups showed significantly improved general condition compared to the SiO(2) model group, with markedly reduced pulmonary fibrosis severity. Early intervention groups demonstrated superior pathological improvement outcomes compared to the late intervention groups. Metabolomics analysis revealed that PCA and OPLS-DA of urine samples demonstrated significant differences in metabolic profiles between the control group and SiO(2) model group. A total of 13 differential metabolites were identified, involving two core metabolic pathways: sphingolipid metabolism and riboflavin metabolism. All intervention groups exhibited a trend of metabolic profiling regression toward the control group, with 5-10 differential metabolites screened out, capable of reversing 2-4 core abnormal metabolite levels. Post-intervention differential metabolites were primarily enriched in four metabolic pathways: sphingolipid metabolism, riboflavin metabolism, unsaturated fatty acid biosynthesis, and linoleic acid metabolism (&lt;i&gt;P&lt;/i&gt;&lt;0.05) . &lt;b&gt;Conclusion:&lt;/b&gt; This study demonstrates that silicosis development is closely associated with dysregulation of sphingolipid and riboflavin metabolic pathways. Targeted interventions can mitigate pulmonary fibrosis damage by restoring core metabolite levels. The PI3K/Akt/HIF-1α signaling pat","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"44 4","pages":"258-266"},"PeriodicalIF":0.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}