Virulence最新文献

{"title":"Emergence of <i>Staphylococcus argenteus</i> in pediatrics: Molecular insights from a hospital in East China.","authors":"Chao Fang, Zheng Zhou, Jianping Li, Mingming Zhou","doi":"10.1080/21505594.2024.2396477","DOIUrl":"10.1080/21505594.2024.2396477","url":null,"abstract":"<p><p><i>Staphylococcus argenteus</i> is a novel species within the <i>Staphylococcus aureus</i> complex and can cause serious bloodstream infections (BSIs) in humans, which have been mainly reported in adults, especially the elderly. In this study, we analyzed the molecular characterization of a strain of <i>S. argenteus</i> (22WJ8192) isolated from the peripheral vein blood sample of a seven-month-old female infant in Eastern China. The 22WJ8192 belonged to sequence type (ST)2250 and harbored six antibiotic-resistance genes and 53 virulence genes and was resistant to penicillin. Additionally, we conducted a comparative analysis of the molecular characteristics of <i>S. argenteus</i> sourced from various origins within the dataset, predominantly from the National Center for Biotechnology Information Collection (NCBI) genome database. Antibiotic-resistance genes <i>blaR1</i>, <i>blaI_of_Z</i>, <i>blaZ</i>, <i>fosB-Saur</i>, <i>tet(L)</i>, <i>aph(3\")-IIIa</i>, <i>mecA</i>, and <i>dfrG</i> were more prevalent among the strains of human origin. Virulence genes <i>lukF-PV</i>, <i>sak</i>, <i>sdrE</i>, <i>scn</i>, <i>sdrC</i>, and <i>sdrD</i> were more prevalent among strains of human origin. The presence of antibiotic-resistance genes <i>blaR1</i>, <i>blaI_of_Z</i>, <i>blaZ</i>, <i>fosB-Saur</i>, and <i>aph(3\")-IIIa</i> in strain 22WJ8192 was also more common among strains of human origin in the dataset. Conversely, the antibiotic-resistance genes <i>tet(L)</i>, <i>mecA</i>, and <i>dfrG</i>, typically found in strains of human origin, were not detected in 22WJ8192. Additionally, virulence genes <i>lukF-PV</i>, <i>sak</i>, <i>sdrE</i>, <i>scn</i>, <i>sdrC</i>, and <i>sdrD</i> present in 22WJ8192 exhibited a higher prevalence among strains of human origin in the dataset. In conclusion, this study emphasizes the potential of <i>S. argenteus</i> ST2250 to induce severe bloodstream infections in infants, shedding light on the molecular characteristics of this strain.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2396477"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TolC facilitates the intracellular survival and immunomodulation of <i>Salmonella</i> Typhi in human host cells.","authors":"Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien","doi":"10.1080/21505594.2024.2395831","DOIUrl":"10.1080/21505594.2024.2395831","url":null,"abstract":"<p><p><i>Salmonella enterica</i> serovar Typhi (<i>S</i>. Typhi) causes typhoid fever, a systemic infection that affects millions of people worldwide. <i>S</i>. Typhi can invade and survive within host cells, such as intestinal epithelial cells and macrophages, by modulating their immune responses. However, the immunomodulatory capability of <i>S</i>. Typhi in relation to TolC-facilitated efflux pump function remains unclear. The role of TolC, an outer membrane protein that facilitates efflux pump function, in the invasion and immunomodulation of <i>S</i>. Typhi, was studied in human intestinal epithelial cells and macrophages. The <i>tolC</i> deletion mutant of <i>S</i>. Typhi was compared with the wild-type and its complemented strain in terms of their ability to invade epithelial cells, survive and induce cytotoxicity in macrophages, and elicit proinflammatory cytokine production in macrophages. The <i>tolC</i> mutant, which has a defective outer membrane, was impaired in invading epithelial cells compared to the wild-type strain, but the intracellular presence of the <i>tolC</i> mutant exhibited greater cytotoxicity and induced higher levels of proinflammatory cytokines (<i>IL-1β</i> and <i>IL-8</i>) in macrophages compared to the wild-type strain. These effects were reversed by complementing the <i>tolC</i> mutant with a functional <i>tolC</i> gene. Our results suggest that TolC plays a role in <i>S</i>. Typhi to efficiently invade epithelial cells and suppress host immune responses during infection. TolC may be a potential target for the development of novel therapeutics against typhoid fever.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2395831"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11385165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Akkermansia muciniphila</i>: A promising probiotic against inflammation and metabolic disorders.","authors":"Yanqing Zhao, Huijun Yang, Peng Wu, Shuguo Yang, Wenkun Xue, Biao Xu, Sirui Zhang, Bin Tang, Daoxiu Xu","doi":"10.1080/21505594.2024.2375555","DOIUrl":"10.1080/21505594.2024.2375555","url":null,"abstract":"<p><p>Metabolic disease is a worldwide epidemic that has become a public health problem. Gut microbiota is considered to be one of the important factors that maintain human health by regulating host metabolism. As an abundant bacterium in the host gut, <i>A. muciniphila</i> regulates metabolic and immune functions, and protects gut health. Multiple studies have indicated that alterations in the abundance of <i>A. muciniphila</i> are associated with various diseases, including intestinal inflammatory diseases, obesity, type 2 diabetes mellitus, and even parasitic diseases. Beneficial effects were observed not only in live <i>A. muciniphila</i>, but also in pasteurized <i>A. muciniphila, A. muciniphila</i>-derived extracellular vesicles, outer membrane, and secreted proteins. Although numerous studies have only proven the simple correlation between multiple diseases and <i>A. muciniphila</i>, an increasing number of studies in animal models and preclinical models have demonstrated that the beneficial impacts shifted from correlations to in-depth mechanisms. In this review, we provide a comprehensive view of the beneficial effects of <i>A. muciniphila</i> on different diseases and summarize the potential mechanisms of action of <i>A. muciniphila</i> in the treatment of diseases. We provide a comprehensive understanding of <i>A. muciniphila</i> for improving host health and discuss the perspectives of <i>A. muciniphila</i> in the future studies.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2375555"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity and virulence of <i>Yersinia</i>.","authors":"Jarett A Seabaugh, Deborah M Anderson","doi":"10.1080/21505594.2024.2316439","DOIUrl":"10.1080/21505594.2024.2316439","url":null,"abstract":"<p><p>The genus <i>Yersinia</i> includes human, animal, insect, and plant pathogens as well as many symbionts and harmless bacteria. Within this genus are <i>Yersinia enterocolitica</i> and the <i>Yersinia pseudotuberculosis</i> complex, with four human pathogenic species that are highly related at the genomic level including the causative agent of plague, <i>Yersinia pestis</i>. Extensive laboratory, field work, and clinical research have been conducted to understand the underlying pathogenesis and zoonotic transmission of these pathogens. There are presently more than 500 whole genome sequences from which an evolutionary footprint can be developed that details shared and unique virulence properties. Whereas the virulence of <i>Y. pestis</i> now seems in apparent homoeostasis within its flea transmission cycle, substantial evolutionary changes that affect transmission and disease severity continue to ndergo apparent selective pressure within the other <i>Yersiniae</i> that cause intestinal diseases. In this review, we will summarize the present understanding of the virulence and pathogenesis of <i>Yersinia</i>, highlighting shared mechanisms of virulence and the differences that determine the infection niche and disease severity.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2316439"},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10896167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adjuvants for <i>Helicobacter pylori</i> vaccines: Outer membrane vesicles provide an alternative strategy.","authors":"Hanchi Zhang, Zhili Liu, Yi Li, Ziwei Tao, Lu Shen, Yinpan Shang, Xiaotian Huang, Qiong Liu","doi":"10.1080/21505594.2024.2425773","DOIUrl":"10.1080/21505594.2024.2425773","url":null,"abstract":"<p><p><i>Helicobacter pylori</i> (<i>H. pylori</i>) is a gram-negative, spiral-shaped bacterium that colonizes the human stomach, leading to various gastric diseases. The efficacy of traditional treatments, such as bismuth-based triple and quadruple therapies, has been reduced due to increasing antibiotic resistance and drug toxicity. As a result, the development of effective vaccines was proposed to control <i>H. pylori</i>-induced infections; however, one of the primary challenges is the lack of potent adjuvants. Although various adjuvants, both toxic (e.g. cholera toxin and <i>Escherichia coli</i> heat-labile toxin) and non-toxic (e.g. aluminum and propolis), have been tested for vaccine development, no clinically favorable adjuvants have been identified due to high toxicity, weak immunostimulatory effects, inability to elicit specific immune responses, or latent side effects. Outer membrane vesicles (OMVs), mainly secreted by gram-negative bacteria, have emerged as promising candidates for <i>H. pylori</i> vaccine adjuvants due to their potential applications. OMVs enhance mucosal immunity and Th1 and Th17 cell responses, which have been recognized to have protective effects and guarantee safety and efficacy. The development of an effective vaccine against <i>H. pylori</i> infection is ongoing, with clinical trials expected in the future.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2425773"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YjbH contributes to <i>Staphylococcus aureus</i> skin pathology and immune response through Agr-mediated α-toxin regulation.","authors":"Aubrey K G McReynolds, Emma A Pagella, Miranda J Ridder, Olivia Rippee, Zachary Clark, Michaella J Rekowski, Michele T Pritchard, Jeffrey L Bose","doi":"10.1080/21505594.2024.2399798","DOIUrl":"10.1080/21505594.2024.2399798","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is the most common cause of skin and soft tissue infections (SSTIs) with Methicillin-Resistant <i>S. aureus</i> (MRSA) strains being a major contributor in both community and hospital settings. <i>S. aureus</i> relies on metabolic diversity and a large repertoire of virulence factors to cause disease. This includes α-hemolysin (Hla), an integral player in tissue damage found in various models, including SSTIs. Previously, we identified a role for the Spx adapter protein, YjbH, in the regulation of several virulence factors and as an inhibitor of pathogenesis in a sepsis model. In this study, we found that YjbH is critical for tissue damage during SSTI, and its absence leads to decreased proinflammatory chemokines and cytokines in the skin. We identified no contribution of YjbI, encoded on the same transcript as YjbH. Using a combination of reporters and quantitative hemolysis assays, we demonstrated that YjbH impacts Hla expression and activity both <i>in vitro</i> and <i>in vivo</i>. Additionally, expression of Hla from a non-native promoter reversed the tissue damage phenotype of the Δ<i>yjbIH</i> mutant. Lastly, we identified reduced Agr activity as the likely cause for reduced Hla production in the Δ<i>yjbH</i> mutant. This work continues to define the importance of YjbH in the pathogenesis of <i>S. aureus</i> infection as well as identify a new pathway important for Hla production.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2399798"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11404607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatic and extrahepatic metabolic modulation in hbv-related decompensated cirrhosis and acute-on-chronic liver failure.","authors":"Zhi-Wei Li, Sheng Tu, Xia Yu, Yi-Jie Wang, Kai Gong, De-Xin Yang, Jun-Jie Yao, Hao-Tang Ren, Da-Xian Wu, Zhe-Hua Zhang, Xiao-Ling Su, Yu Wang, Zhao-Yi Pan, Rui-Hong Zhao, Ji-Fang Sheng, Yun-Qing Qiu, Yu Shi, Ze-Yu Sun","doi":"10.1080/21505594.2024.2404953","DOIUrl":"10.1080/21505594.2024.2404953","url":null,"abstract":"<p><p>Acute-on-chronic liver failure (ACLF) and decompensated cirrhosis (DC) are life-threatening syndromes that can develop at the end-stage of chronic hepatitis B virus (HBV) infection. Both ACLF and DC are complicated by hepatic and extrahepatic pathogeneses. To better understand the compartment-specific metabolic modulations related to their pathogenesis, HBV-DC, HBV-ACLF patients, and controls (30 each) were analyzed by metabolomics using portal (Port), hepatic vein (Hep), and peripheral (Peri) serum. Compartment ratios of metabolites (Ratio_Hep/Port, Ratio_Peri/Hep, and Ratio_Port/Peri) were calculated. The liver tissues (10 per group) were analyzed using transcriptomics and metabolomics. An additional 75 patients with ACLF, 20 with DC, and 20 with liver cirrhosis (LC) were used to confirm oxlipid dysregulation. Both multi-omics datasets suggest suppressed energy, amino acid, and pyrimidine metabolism in the ACLF/DC liver. The serum metabolomic variations were contributed primarily by disease rather than sampling compartments, as both HBV-ACLF and HBV-DC patients demonstrated abnormal profiles of amino acids and peptides, indoles, purines, steroids, and benzimidazoles. In ACLF/DC patients, impaired hepatic metabolism resulted in a highly correlated hepatic and portal vein serum metabolome and release of inflammatory lipids and heme metabolites from the liver. HBV-ACLF showed higher Ratio_Peri/Hep of extrahepatic inflammatory oxlipids, while HBV-DC patients showed higher Ratio_Port/Peri of gut microbial metabolites. An inflammatory oxlipid outburst was confirmed in the early stages of HBV-ACLF. The inflammatory effects of the selected oxlipids were confirmed in monocytes. These findings support a synergy between liver-specific mechanisms and systemic inflammation in ACLF/DC development, and that pro-inflammatory oxlipids are metabolic signatures of early HBV-ACLF.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2404953"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A <i>Colletotrichum fructicola</i> dual specificity phosphatase CfMsg5 is regulated by the CfAp1 transcription factor during oxidative stress and promotes virulence on <i>Camellia oleifera</i>.","authors":"Yalan Gao, Shengpei Zhang, Song Sheng, He Li","doi":"10.1080/21505594.2024.2413851","DOIUrl":"10.1080/21505594.2024.2413851","url":null,"abstract":"<p><p>Anthracnose, caused by <i>Colletotrichum</i> species, induces significant economic damages to crop plants annually, especially for <i>Camellia oleifera</i>. During infection, the counter-defence mechanisms of plant pathogens against ROS-mediated resistance, however, remain poorly understood. By employing Weighted Gene Co-expression Network Analysis (WGCNA), we identified ACTIVATOR PROTEIN-1 (AP-1), a bZIP transcription factor, as significant to infection. And deletion of <i>CfAP1</i> inhibited aerial hyphae formation and growth under oxidative stress. Furthermore, RNA-seq analysis post H₂O₂ treatment revealed 33 significantly down-regulated genes in the AP-1 deficient strain, including A12032, a dual specificity phosphatase (DSP) homologous to MSG5 from <i>Saccharomyces cerevisiae</i>. This Δ<i>Cfmsg5</i> strain showed enhanced oxidative tolerance, reduced ROS scavenging, and negative regulation of the CWI MAPK cascade under oxygen stress, suggesting its involvement in oxidative signal transduction. Importantly, we provide evidence that CfMsg5 regulates growth, endoplasmic reticulum stress, and several unfolded protein response genes upregulated in Δ<i>Cfmsg5</i>. Collectively, this study identified core components during <i>C. fructicola</i> infection and highlights a potential regulatory module involving CfAp1 and CfMsg5 in response to host ROS bursts. It provides new insights into fungal infection mechanisms and potential targets like <i>CfAP1</i> and <i>CfMSG5</i> for managing anthracnose diseases.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2413851"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host combats porcine reproductive and respiratory syndrome virus infection at non-coding RNAs level.","authors":"Zhi Qin, Weiye Liu, Zhihua Qin, Hongliang Zhang, Xuewei Huang","doi":"10.1080/21505594.2024.2416551","DOIUrl":"10.1080/21505594.2024.2416551","url":null,"abstract":"<p><p>Porcine reproductive and respiratory syndrome virus (PRRSV) poses a significant threat to the global swine industry. The emergence of new, highly virulent strains has precipitated recurrent outbreaks worldwide, underscoring the ongoing battle between host and virus. Thus, there is an imperative to formulate a more comprehensive and effective disease control strategy. Studies have shown that host non-coding RNA (ncRNA) is an important regulator of host - virus interactions in PRRSV infection. Hence, a thorough comprehension of the roles played by ncRNAs in PRRSV infection can augment our understanding of the pathogenic mechanisms underlying PRRSV infection. This review focuses on elucidating contemporary insights into the roles of host microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in PRRSV infection, providing both theoretical foundations and fresh perspectives for ongoing research into the mechanisms driving PRRSV infection and its pathogenesis.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2416551"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden liver-joint axis: HBV infection causes rheumatoid arthritis via TRAFD1 with imbalance of HBV X protein and trans-ferulic acid.","authors":"Na Zhao, Xiaoyu Zhang, Xilong Wang, Zhipu Liu, Guixi Zheng, Xin Zhang, Helgi B Schiöth, Chengxi Sun, Hongxing Wang, Yi Zhang","doi":"10.1080/21505594.2024.2422540","DOIUrl":"10.1080/21505594.2024.2422540","url":null,"abstract":"<p><p>Liver metabolites are involved in the progression of rheumatoid arthritis (RA), indicating a connection between the liver and joints. However, the impact and mechanism of Hepatitis B virus (HBV), a hepatotropic virus, on RA are still unclear. We investigated the correlation between HBV and RA using Mendelian randomization analysis. Single-cell transcriptome analysis was conducted to investigate changes in cell subtypes in synovial tissue of HBV-RA patients. Fibroblast-like synoviocytes (FLS) were used to create a cell model, and the transcriptome was examined to identify the key downstream molecules of FMT regulated by HBx. CIA model was constructed using HBV transgenic, HBx transgenic, and TRADF1 knockout mice to investigate the impact and mechanism of HBV on CIA. The results of our study revealed a significant positive correlation between HBV and RA. The functional studies identified a crucial role of fibroblast-myofibroblast transition (FMT) in the progression of RA. The results suggest that HBV-encoded HBx may promote FMT in RA by upregulating TRAFD1. Furthermore, trans-ferulic acid (TFA) was identified by screening for common metabolites in the liver, joints, and peripheral blood using the metabolome and WGCNA. Interestingly, we found that TFA ameliorated HBx-induced RA by suppressing TRAFD1 expression. Our study demonstrates that hidden liver-joint axis, an imbalance between TFA and HBx, plays a critical role in HBV-induced RA, which could be a potential strategy for preventing RA development.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2422540"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}