Virulence最新文献_第2页

TolC facilitates the intracellular survival and immunomodulation of Salmonella Typhi in human host cells. TolC 有助于伤寒沙门氏菌在人类宿主细胞中的胞内存活和免疫调节。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-09-09 DOI: 10.1080/21505594.2024.2395831

Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien

{"title":"TolC facilitates the intracellular survival and immunomodulation of Salmonella Typhi in human host cells.","authors":"Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien","doi":"10.1080/21505594.2024.2395831","DOIUrl":"10.1080/21505594.2024.2395831","url":null,"abstract":"Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever, a systemic infection that affects millions of people worldwide. S. Typhi can invade and survive within host cells, such as intestinal epithelial cells and macrophages, by modulating their immune responses. However, the immunomodulatory capability of S. Typhi in relation to TolC-facilitated efflux pump function remains unclear. The role of TolC, an outer membrane protein that facilitates efflux pump function, in the invasion and immunomodulation of S. Typhi, was studied in human intestinal epithelial cells and macrophages. The tolC deletion mutant of S. Typhi was compared with the wild-type and its complemented strain in terms of their ability to invade epithelial cells, survive and induce cytotoxicity in macrophages, and elicit proinflammatory cytokine production in macrophages. The tolC mutant, which has a defective outer membrane, was impaired in invading epithelial cells compared to the wild-type strain, but the intracellular presence of the tolC mutant exhibited greater cytotoxicity and induced higher levels of proinflammatory cytokines (IL-1β and IL-8) in macrophages compared to the wild-type strain. These effects were reversed by complementing the tolC mutant with a functional tolC gene. Our results suggest that TolC plays a role in S. Typhi to efficiently invade epithelial cells and suppress host immune responses during infection. TolC may be a potential target for the development of novel therapeutics against typhoid fever.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila: A promising probiotic against inflammation and metabolic disorders. Akkermansia muciniphila：一种很有前景的抗炎症和代谢紊乱的益生菌。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI: 10.1080/21505594.2024.2375555

Yanqing Zhao, Huijun Yang, Peng Wu, Shuguo Yang, Wenkun Xue, Biao Xu, Sirui Zhang, Bin Tang, Daoxiu Xu

{"title":"Akkermansia muciniphila: A promising probiotic against inflammation and metabolic disorders.","authors":"Yanqing Zhao, Huijun Yang, Peng Wu, Shuguo Yang, Wenkun Xue, Biao Xu, Sirui Zhang, Bin Tang, Daoxiu Xu","doi":"10.1080/21505594.2024.2375555","DOIUrl":"10.1080/21505594.2024.2375555","url":null,"abstract":"Metabolic disease is a worldwide epidemic that has become a public health problem. Gut microbiota is considered to be one of the important factors that maintain human health by regulating host metabolism. As an abundant bacterium in the host gut, A. muciniphila regulates metabolic and immune functions, and protects gut health. Multiple studies have indicated that alterations in the abundance of A. muciniphila are associated with various diseases, including intestinal inflammatory diseases, obesity, type 2 diabetes mellitus, and even parasitic diseases. Beneficial effects were observed not only in live A. muciniphila, but also in pasteurized A. muciniphila, A. muciniphila-derived extracellular vesicles, outer membrane, and secreted proteins. Although numerous studies have only proven the simple correlation between multiple diseases and A. muciniphila, an increasing number of studies in animal models and preclinical models have demonstrated that the beneficial impacts shifted from correlations to in-depth mechanisms. In this review, we provide a comprehensive view of the beneficial effects of A. muciniphila on different diseases and summarize the potential mechanisms of action of A. muciniphila in the treatment of diseases. We provide a comprehensive understanding of A. muciniphila for improving host health and discuss the perspectives of A. muciniphila in the future studies.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of Staphylococcus argenteus in pediatrics: Molecular insights from a hospital in East China. 儿科中出现的金黄色葡萄球菌：来自华东一家医院的分子研究。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI: 10.1080/21505594.2024.2396477

Chao Fang, Zheng Zhou, Jianping Li, Mingming Zhou

{"title":"Emergence of Staphylococcus argenteus in pediatrics: Molecular insights from a hospital in East China.","authors":"Chao Fang, Zheng Zhou, Jianping Li, Mingming Zhou","doi":"10.1080/21505594.2024.2396477","DOIUrl":"10.1080/21505594.2024.2396477","url":null,"abstract":"Staphylococcus argenteus is a novel species within the Staphylococcus aureus complex and can cause serious bloodstream infections (BSIs) in humans, which have been mainly reported in adults, especially the elderly. In this study, we analyzed the molecular characterization of a strain of S. argenteus (22WJ8192) isolated from the peripheral vein blood sample of a seven-month-old female infant in Eastern China. The 22WJ8192 belonged to sequence type (ST)2250 and harbored six antibiotic-resistance genes and 53 virulence genes and was resistant to penicillin. Additionally, we conducted a comparative analysis of the molecular characteristics of S. argenteus sourced from various origins within the dataset, predominantly from the National Center for Biotechnology Information Collection (NCBI) genome database. Antibiotic-resistance genes blaR1, blaI_of_Z, blaZ, fosB-Saur, tet(L), aph(3\")-IIIa, mecA, and dfrG were more prevalent among the strains of human origin. Virulence genes lukF-PV, sak, sdrE, scn, sdrC, and sdrD were more prevalent among strains of human origin. The presence of antibiotic-resistance genes blaR1, blaI_of_Z, blaZ, fosB-Saur, and aph(3\")-IIIa in strain 22WJ8192 was also more common among strains of human origin in the dataset. Conversely, the antibiotic-resistance genes tet(L), mecA, and dfrG, typically found in strains of human origin, were not detected in 22WJ8192. Additionally, virulence genes lukF-PV, sak, sdrE, scn, sdrC, and sdrD present in 22WJ8192 exhibited a higher prevalence among strains of human origin in the dataset. In conclusion, this study emphasizes the potential of S. argenteus ST2250 to induce severe bloodstream infections in infants, shedding light on the molecular characteristics of this strain.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli and HPV16 coinfection may contribute to the development of cervical cancer. 大肠杆菌和 HPV16 共同感染可能会导致宫颈癌的发生。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-02-21 DOI: 10.1080/21505594.2024.2319962

Qin Zou, Yingying Wu, ShuaiShuai Zhang, Shu Li, Siyue Li, Yan Su, Lei Zhang, Qian Li, Hua Zou, Xinyuan Zhang, Teng Wang, Shuang Liang, Jun Yang, Chunli Li

{"title":"Escherichia coli and HPV16 coinfection may contribute to the development of cervical cancer.","authors":"Qin Zou, Yingying Wu, ShuaiShuai Zhang, Shu Li, Siyue Li, Yan Su, Lei Zhang, Qian Li, Hua Zou, Xinyuan Zhang, Teng Wang, Shuang Liang, Jun Yang, Chunli Li","doi":"10.1080/21505594.2024.2319962","DOIUrl":"10.1080/21505594.2024.2319962","url":null,"abstract":"Persistent human papillomavirus HPV infection is a necessary but insufficient condition for cervical cancer. Microorganisms are crucial environmental factors in cancers susceptibility and progression, recently attracting considerable attention. This study aimed to determine the infection status and relationship between high-risk HPV (HR-HPV) and lower genital tract infectious pathogens in cervical cancer and its precursors. From a retrospective and a prospective cohort analysis, Escherichia coli (E. coli) dominated the pathogens isolated from cervical discharges, and an isolation rate uptrend has been shown recently. HPV16 and E. coli's coinfection rate gradually increased with the severity of cervical intraepithelial neoplasia. The adhesion and invasion abilities of the isolated E. coli to HPV16-positive SiHa cells were evaluated in vitro. The TCGA database and cervical tissues samples analysis showed that IL-10 was upregulated in cervical cancer. IL-10 expression levels increased in tissue samples with the severity of cervical cancer and its precursors with HPV16 and E. coli coinfection. Although no significant changes in IL-10 production were observed in the co-culture supernatant, we hypothesized that Treg immune cells in the tumour microenvironment might be responsible for the local IL-10 upregulation, according to our data showing Foxp3 upregulation and an upward trend with the cervical intraepithelial neoplasia grading to cancer and tumours with E. coli and HPV16 coinfection. Our data provide insights into the possible role of E. coli in cervical cancer progression and suggest that the application of HPV and E. coli screening programs may be an effective strategy to relieve the burden of cervical cancer and its precursor lesions.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10883084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139913584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenicity and virulence of Yersinia. 耶尔森氏菌的致病性和毒性。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-02-22 DOI: 10.1080/21505594.2024.2316439

Jarett A Seabaugh, Deborah M Anderson

{"title":"Pathogenicity and virulence of Yersinia.","authors":"Jarett A Seabaugh, Deborah M Anderson","doi":"10.1080/21505594.2024.2316439","DOIUrl":"10.1080/21505594.2024.2316439","url":null,"abstract":"The genus Yersinia includes human, animal, insect, and plant pathogens as well as many symbionts and harmless bacteria. Within this genus are Yersinia enterocolitica and the Yersinia pseudotuberculosis complex, with four human pathogenic species that are highly related at the genomic level including the causative agent of plague, Yersinia pestis. Extensive laboratory, field work, and clinical research have been conducted to understand the underlying pathogenesis and zoonotic transmission of these pathogens. There are presently more than 500 whole genome sequences from which an evolutionary footprint can be developed that details shared and unique virulence properties. Whereas the virulence of Y. pestis now seems in apparent homoeostasis within its flea transmission cycle, substantial evolutionary changes that affect transmission and disease severity continue to ndergo apparent selective pressure within the other Yersiniae that cause intestinal diseases. In this review, we will summarize the present understanding of the virulence and pathogenesis of Yersinia, highlighting shared mechanisms of virulence and the differences that determine the infection niche and disease severity.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10896167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The long noncoding RNA loc107053557 acts as a gga-miR-3530-5p sponge to suppress the replication of vvIBDV through regulating STAT1 expression. 长非编码 RNA loc107053557 可作为 gga-miR-3530-5p 海绵，通过调节 STAT1 的表达来抑制 vvIBDV 的复制。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-03-31 DOI: 10.1080/21505594.2024.2333237

Xuewei Huang, Yue Li, Jiaxuan Li, Yanping Jiang, Wen Cui, Han Zhou, Lijie Tang

{"title":"The long noncoding RNA loc107053557 acts as a gga-miR-3530-5p sponge to suppress the replication of vvIBDV through regulating STAT1 expression.","authors":"Xuewei Huang, Yue Li, Jiaxuan Li, Yanping Jiang, Wen Cui, Han Zhou, Lijie Tang","doi":"10.1080/21505594.2024.2333237","DOIUrl":"10.1080/21505594.2024.2333237","url":null,"abstract":"Infectious bursal disease virus (IBDV) causes immunosuppression and high mortality in young chickens. Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are important regulators during viral infection. However, detailed the regulatory mechanisms of lncRNA-miRNA-mRNA have not yet been described in IBDV infection. Here, we analysed the role of lncRNA53557/gga-miR-3530-5p/STAT1 axis in very virulent IBDV (vvIBDV) infection. Evidently upregulated expression of lncRNA53557 was observed in bursa of Fabricius and DT40 cells. Meanwhile, overexpression of lncRNA53557 promoted STAT1 expression and inhibited vvIBDV replication and vice versa, indicating that the upregulation of lncRNA53557 was part of the host antiviral defence. The subcellular fractionation assay confirmed that lncRNA53557 can be localized in the cytoplasm. Further, dual-luciferase reporter, RNA pulldown, FISH and RT-qPCR assays revealed that lncRNA53557 were directly bound to gga-miR-3530-5p and had a negative regulatory relationship between them. Subsequent mechanistic analysis showed that lncRNA53557 acted as a competing endogenous RNA (ceRNA) of gga-miR-3530-5p to relieve the repressive effect of gga-miR-3530-5p on its target STAT1, as well as Mx1, OASL, and ISG15, thereby suppressing vvIBDV replication. The study reveals that a network of enriched lncRNAs and lncRNA-associated ceRNA is involved in the regulation of IBDV infection, offering new insight into the mechanisms underlying IBDV-host interaction.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ser/Thr protein kinase Stk1 phosphorylates the key transcriptional regulator AlgR to modulate virulence and resistance in Pseudomonas aeruginosa. Ser/Thr 蛋白激酶 Stk1 磷酸化关键转录调节因子 AlgR，从而调节铜绿假单胞菌的毒力和抗性。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-20 DOI: 10.1080/21505594.2024.2367649

Rui Li, Xuan Zhu, Pengfei Zhang, Xuan Wu, Qian Jin, Jianyi Pan

{"title":"Ser/Thr protein kinase Stk1 phosphorylates the key transcriptional regulator AlgR to modulate virulence and resistance in Pseudomonas aeruginosa.","authors":"Rui Li, Xuan Zhu, Pengfei Zhang, Xuan Wu, Qian Jin, Jianyi Pan","doi":"10.1080/21505594.2024.2367649","DOIUrl":"10.1080/21505594.2024.2367649","url":null,"abstract":"Pseudomonas aeruginosa is one of the leading causes of nosocomial infections worldwide and has emerged as a serious public health threat, due in large part to its multiple virulence factors and remarkable resistance capabilities. Stk1, a eukaryotic-type Ser/Thr protein kinase, has been shown in our previous work to be involved in the regulation of several signalling pathways and biological processes. Here, we demonstrate that deletion of stk1 leads to alterations in several virulence- and resistance-related physiological functions, including reduced pyocyanin and pyoverdine production, attenuated twitching motility, and enhanced biofilm production, extracellular polysaccharide secretion, and antibiotic resistance. Moreover, we identified AlgR, an important transcriptional regulator, as a substrate for Stk1, with its phosphorylation at the Ser143 site catalysed by Stk1. Intriguingly, both the deletion of stk1 and the mutation of Ser143 of AlgR to Ala result in similar changes in the above-mentioned physiological functions. Furthermore, assays of algR expression in these strains suggest that changes in the phosphorylation state of AlgR, rather than its expression level, underlie changes in these physiological functions. These findings uncover Stk1-mediated phosphorylation of AlgR as an important mechanism for regulating virulence and resistance in P. aeruginosa.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11197903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The presence of differentiated C2C12 muscle cells enhances toxin production and growth by Clostridium perfringens type A strain ATCC3624. 分化的 C2C12 肌肉细胞可促进 A 型产气荚膜梭菌 ATCC3624 的毒素产生和生长。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI: 10.1080/21505594.2024.2388219

Jihong Li, Sameera Sayeed, Bruce A McClane

{"title":"The presence of differentiated C2C12 muscle cells enhances toxin production and growth by Clostridium perfringens type A strain ATCC3624.","authors":"Jihong Li, Sameera Sayeed, Bruce A McClane","doi":"10.1080/21505594.2024.2388219","DOIUrl":"10.1080/21505594.2024.2388219","url":null,"abstract":"Clostridium perfringens type A causes gas gangrene, which involves muscle infection. Both alpha toxin (PLC), encoded by the plc gene, and perfringolysin O (PFO), encoded by the pfoA gene, are important when type A strains cause gas gangrene in a mouse model. This study used the differentiated C2C12 muscle cell line to test the hypothesis that one or both of those toxins contributes to gas gangrene pathogenesis by releasing growth nutrients from muscle cells. RT-qPCR analyses showed that the presence of differentiated C2C12 cells induces C. perfringens type A strain ATCC3624 to upregulate plc and pfoA expression, as well as increase expression of several regulatory genes, including virS/R, agrB/D, and eutV/W. The VirS/R two component regulatory system (TCRS) and its coupled Agr-like quorum sensing system, along with the EutV/W TCRS (which regulates expression of genes involved in ethanolamine [EA] utilization), were shown to mediate the C2C12 cell-induced increase in plc and pfoA expression. EA was demonstrated to increase toxin gene expression. ATCC3624 growth increased in the presence of differentiated C2C12 muscle cells and this effect was shown to involve both PFO and PLC. Those membrane-active toxins were each cytotoxic for differentiated C2C12 cells, suggesting they support ATCC3624 growth by releasing nutrients from differentiated C2C12 cells. These findings support a model where, during gas gangrene, increased production of PFO and PLC in the presence of muscle cells causes more damage to those host cells, which release nutrients like EA that are then used to support C. perfringens growth in muscle.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent global outbreaks of highly pathogenic and low-pathogenicity avian influenza A virus infections. 近期全球爆发的高致病性和低致病性甲型禽流感病毒感染。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-07-25 DOI: 10.1080/21505594.2024.2383478

Hinh Ly

引用次数: 0

The complex interplay between encephalomyocarditis virus and the host defence system. 脑心肌炎病毒与宿主防御系统之间复杂的相互作用。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-07-27 DOI: 10.1080/21505594.2024.2383559

Jingying Xie, Adi Idris, Ruofei Feng

引用次数: 0