IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-25 DOI: 10.1080/21505594.2024.2359470

Xiaoyan Zhang, Yuying Zhang, Fanhua Wei

引用次数: 0

Throat microbiota drives alterations in pulmonary alveolar microbiota in patients with septic ARDS. 咽喉微生物群驱动脓毒症 ARDS 患者肺泡微生物群的改变。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-05-12 DOI: 10.1080/21505594.2024.2350775

Na-Na Li, Kai Kang, Yang Zhou, Yan-Qi Liu, Qian-Qian Zhang, Pei-Yao Luo, Lei Wang, Ming-Yin Man, Jia-Feng Lv, Xi-Bo Wang, Ya-Hui Peng, Fei-Yu Luan, Yue Li, Jian-Nan Zhang, Yang Chong, Yi-Qi Wang, Chang-Song Wang, Ming-Yan Zhao, Kai-Jiang Yu

{"title":"Throat microbiota drives alterations in pulmonary alveolar microbiota in patients with septic ARDS.","authors":"Na-Na Li, Kai Kang, Yang Zhou, Yan-Qi Liu, Qian-Qian Zhang, Pei-Yao Luo, Lei Wang, Ming-Yin Man, Jia-Feng Lv, Xi-Bo Wang, Ya-Hui Peng, Fei-Yu Luan, Yue Li, Jian-Nan Zhang, Yang Chong, Yi-Qi Wang, Chang-Song Wang, Ming-Yan Zhao, Kai-Jiang Yu","doi":"10.1080/21505594.2024.2350775","DOIUrl":"10.1080/21505594.2024.2350775","url":null,"abstract":"Objectives: The translocation of intestinal flora has been linked to the colonization of diverse and heavy lower respiratory flora in patients with septic ARDS, and is considered a critical prognostic factor for patients.Methods: On the first and third days of ICU admission, BALF, throat swab, and anal swab were collected, resulting in a total of 288 samples. These samples were analyzed using 16S rRNA analysis and the traceability analysis of new generation technology.Results: On the first day, among the top five microbiota species in abundance, four species were found to be identical in BALF and throat samples. Similarly, on the third day, three microbiota species were found to be identical in abundance in both BALF and throat samples. On the first day, 85.16% of microorganisms originated from the throat, 5.79% from the intestines, and 9.05% were unknown. On the third day, 83.52% of microorganisms came from the throat, 4.67% from the intestines, and 11.81% were unknown. Additionally, when regrouping the 46 patients, the results revealed a significant predominance of throat microorganisms in BALF on both the first and third day. Furthermore, as the disease progressed, the proportion of intestinal flora in BALF increased in patients with enterogenic ARDS.Conclusions: In patients with septic ARDS, the main source of lung microbiota is primarily from the throat. Furthermore, the dynamic trend of the microbiota on the first and third day is essentially consistent.It is important to note that the origin of the intestinal flora does not exclude the possibility of its origin from the throat.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11093027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and immunogenicity of CoronaVac in healthy adults: A prospective observational multicenter real-world study in Henan Province, China. CoronaVac 在健康成人中的安全性和免疫原性：中国河南省前瞻性多中心真实世界观察研究。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-02-07 DOI: 10.1080/21505594.2024.2310450

Benchen Rao, Ling Wang, Mengzhao Yang, Hong Luo, Junyi Sun, Shanshuo Liu, Haiyu Wang, Xuemei Wang, Lei Li, Chengyu Yuan, Zujiang Yu, Zhigang Ren

{"title":"Safety and immunogenicity of CoronaVac in healthy adults: A prospective observational multicenter real-world study in Henan Province, China.","authors":"Benchen Rao, Ling Wang, Mengzhao Yang, Hong Luo, Junyi Sun, Shanshuo Liu, Haiyu Wang, Xuemei Wang, Lei Li, Chengyu Yuan, Zujiang Yu, Zhigang Ren","doi":"10.1080/21505594.2024.2310450","DOIUrl":"10.1080/21505594.2024.2310450","url":null,"abstract":"Vaccination has emerged as the primar approach for managing the COVID-19 pandemic. Despite certain clinical trials reporting the safety and immunogenicity of CoronaVac, additional multicenter real-world studies are still necessary. In this study, we recruited 506 healthy volunteers who were not infected with COVID-19 or vaccinated. Each participant provided peripheral blood samples three times: prior to the first dose of vaccine, prior to the second dose, and 8 weeks following the second dose. Ultimately, 388 participants completed the entire follow-up process. No serious adverse events were observed among any of the participants. Within 1 week of vaccination, 13.4% of participants experienced systemic adverse reactions, with fatigue (5.93%) and dizziness (3.35%) being the most frequent. Although some clinical indicators, including creatinine, significantly changed after vaccination (p < 0.05), the mean of all altered indicators remained within the normal range. The positive rates of neutralizing antibodies (NAb), IgG, and IgM were 12.3%, 18.85%, and 5.24% prior to the second dose, respectively; and 57.99%, 86.34%, and 2.32% at 8 weeks following the second dose, respectively. Additionally, seven indicators, such as sex, age, and BMI, were significantly correlated with NAb (p < 0.05). Finally, a prediction model was developed based on age, monocytes, and alanine aminotransferase (ALT) with an AUC value of 87.56% in the train set and 80.71% in the test set. This study demonstrated that safety and immunogenicity of CoronaVac were good. The prediction model based on the baseline clinical characteristics prior to vaccination can help to develop more suitable vaccination strategies.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhinovirus infection and co-infection in children with severe acute respiratory infection during the COVID-19 pandemic period. COVID-19 大流行期间严重急性呼吸道感染儿童的鼻病毒感染和合并感染。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-02-21 DOI: 10.1080/21505594.2024.2310873

Célia Regina Malveste Ito, Mônica Oliveira Santos, Marcos de Oliveira Cunha, Kelliane Martins de Araújo, Guilherme Rocha Lino de Souza, Geovana Sôffa Rézio, Pollyanna Neta de Brito, Alana Parreira Costa Rezende, Jakeline Godinho Fonseca, Isabela Jubé Wastowski, José Daniel Gonçalves Vieira, Melissa Ameloti Gomes Avelino, Lilian Carla Carneiro

{"title":"Rhinovirus infection and co-infection in children with severe acute respiratory infection during the COVID-19 pandemic period.","authors":"Célia Regina Malveste Ito, Mônica Oliveira Santos, Marcos de Oliveira Cunha, Kelliane Martins de Araújo, Guilherme Rocha Lino de Souza, Geovana Sôffa Rézio, Pollyanna Neta de Brito, Alana Parreira Costa Rezende, Jakeline Godinho Fonseca, Isabela Jubé Wastowski, José Daniel Gonçalves Vieira, Melissa Ameloti Gomes Avelino, Lilian Carla Carneiro","doi":"10.1080/21505594.2024.2310873","DOIUrl":"10.1080/21505594.2024.2310873","url":null,"abstract":"Rhinovirus causes respiratory tract infections in children and is found in co-infections. The objective of this research was to study the clinical profile of rhinovirus infection and co-infection in children with severe acute respiratory infection (SARI) during the COVID-19 pandemic period. We included 606 children ranging in age from 0.1 to 144 months of age from March 2020 to December 2021, hospitalized in the Pediatric Intensive Care Unit (PICU). The samples were collected by secretion from the nasopharynx region. A total of 259 children were tested positive for viral infection, 153 (59.07%) of them had a single rhinovirus infection and, 56 (36.6%) were aged between 60.1 and 144 months. Nine types of co-infections were identified and were found coinfection with three or more viruses (22/104, 21.15%). Observing the seasonality, the number of cases was similar between 2020 (49.53%) and 2021 (51.47%). Patients with a single infection (86.88%) and coinfection (67.30%) were more likely to have coughed. Patients with co-infection required the use of O2 for longer than those with a single rhinovirus infection. Hemogram results obtained from individuals with a single infection had higher levels of urea when compared to patients with co-infection with and other respiratory viruses. Multiple correspondence analyses indicated different clinical symptoms and comorbidities in patients with co-infection compared to those with single infection. The results found that the rhinovirus was much prevalent virus during the pandemic period and was found in co-infection with other virus types, what is important to diagnostic for the correct treatment of patients.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10885176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dry eye disease caused by viral infection: Past, present and future. 由病毒感染引起的干眼病:过去、现在和未来。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2023-12-04 DOI: 10.1080/21505594.2023.2289779

Min Wu, Cuilian Sun, Qin Shi, Yalu Luo, Ziyu Wang, Jianxiang Wang, Yun Qin, Weihang Cui, Chufeng Yan, Huangyi Dai, Zhiyang Wang, Jia Zeng, Yamei Zhou, Manhui Zhu, Xiaojuan Liu

引用次数: 0

Pathogenicity and virulence of Acinetobacter baumannii: Factors contributing to the fitness in healthcare settings and the infected host. 鲍曼不动杆菌的致病性和毒性：医疗机构和受感染宿主的致病因素。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2023-12-06 DOI: 10.1080/21505594.2023.2289769

Massimiliano Lucidi, Daniela Visaggio, Antonella Migliaccio, Giulia Capecchi, Paolo Visca, Francesco Imperi, Raffaele Zarrilli

{"title":"Pathogenicity and virulence of Acinetobacter baumannii: Factors contributing to the fitness in healthcare settings and the infected host.","authors":"Massimiliano Lucidi, Daniela Visaggio, Antonella Migliaccio, Giulia Capecchi, Paolo Visca, Francesco Imperi, Raffaele Zarrilli","doi":"10.1080/21505594.2023.2289769","DOIUrl":"10.1080/21505594.2023.2289769","url":null,"abstract":"Acinetobacter baumannii is a common cause of healthcare-associated infections and hospital outbreaks, particularly in intensive care units. Much of the success of A. baumannii relies on its genomic plasticity, which allows rapid adaptation to adversity and stress. The capacity to acquire novel antibiotic resistance determinants and the tolerance to stresses encountered in the hospital environment promote A. baumannii spread among patients and long-term contamination of the healthcare setting. This review explores virulence factors and physiological traits contributing to A. baumannii infection and adaptation to the hospital environment. Several cell-associated and secreted virulence factors involved in A. baumannii biofilm formation, cell adhesion, invasion, and persistence in the host, as well as resistance to xeric stress imposed by the healthcare settings, are illustrated to give reasons for the success of A. baumannii as a hospital pathogen.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2023-12-13 DOI: 10.1080/21505594.2023.2289756

引用次数: 0

Evaluation of the immune responses of biological adjuvant bivalent vaccine with three different insertion modes for ND and IBD. 评估具有三种不同插入模式的 ND 和 IBD 生物佐剂二价疫苗的免疫反应。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-08-05 DOI: 10.1080/21505594.2024.2387181

Wenying Sun, Shuang Li, Dun Niu, Ruihan Qin, Huimin Li, Zhiqiang Xue, Yunpeng Guo, Jinmiao Liu, Yijia Liu, Xinghao Jiang, Jiechao Yin, Xiaochen Guo, Guiping Ren

{"title":"Evaluation of the immune responses of biological adjuvant bivalent vaccine with three different insertion modes for ND and IBD.","authors":"Wenying Sun, Shuang Li, Dun Niu, Ruihan Qin, Huimin Li, Zhiqiang Xue, Yunpeng Guo, Jinmiao Liu, Yijia Liu, Xinghao Jiang, Jiechao Yin, Xiaochen Guo, Guiping Ren","doi":"10.1080/21505594.2024.2387181","DOIUrl":"10.1080/21505594.2024.2387181","url":null,"abstract":"Infectious bursal disease (IBD) is a widespread problem in the poultry industry, and vaccination is the primary preventive method. However, moderately virulent vaccines may damage the bursa, necessitating the development of a safe and effective vaccine. The Newcastle disease virus (NDV) has been explored as a vector for vaccine development. In this study, reverse genetic technology was used to obtain three recombinant viruses, namely, rClone30-VP2L (P/M)-chGM-CSF (NP), rClone30-chGM-CSF (P/M)-VP2L (NP), and rClone30-VP2L-chGM-CSF (P/M). Animal experiments showed that the three biological adjuvant bivalent vaccines effectively increased anti-NDV and anti-infectious bursal disease virus (IBDV) titres, enhancing both humoral and cellular immune responses in chickens without leading to any harm. Amongst the three biological adjuvant bivalent vaccines, the rClone30-chGM-CSF (P/M)-VP2L (NP) group had higher levels of anti-NDV antibodies at 14 days after the first immunization and stimulated a greater humoral immune response in 7-10 days. While, the rClone30-VP2L (P/M)-chGM-CSF (NP) group was the most effective in producing a higher level of IBDV antibody response. In conclusion, these three vaccines can induce immune responses more rapidly and effectively, streamline production processes, be cost-effective, and provide a new avenue for the development of Newcastle disease (ND) and IBD bivalent vaccines.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11302549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential roles of Rad6 in conidial property, stress tolerance, and pathogenicity of Beauveria bassiana. Rad6 在 Beauveria bassiana 的分生孢子特性、应激耐受性和致病性中的重要作用。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-11 DOI: 10.1080/21505594.2024.2362748

Yi Guan, Haomin He, Yuhan Guo, Longbin Zhang

{"title":"Essential roles of Rad6 in conidial property, stress tolerance, and pathogenicity of Beauveria bassiana.","authors":"Yi Guan, Haomin He, Yuhan Guo, Longbin Zhang","doi":"10.1080/21505594.2024.2362748","DOIUrl":"10.1080/21505594.2024.2362748","url":null,"abstract":"Rad6 functions as a ubiquitin-conjugating protein that regulates cellular processes in many fungal species. However, its role in filamentous entomopathogenic fungi remains poorly understood. This study characterizes Rad6 in Beauveria bassiana, a filamentous fungus widely employed as a critical fungicide globally. The results demonstrate a significant association between Rad6 and conidial properties, heat shock response, and UV-B tolerance. Concurrently, the mutant strain exhibited heightened sensitivity to oxidative stress, cell wall interfering agents, DNA damage stress, and prolonged heat shock. Furthermore, the absence of Rad6 significantly extended the median lethal time (LT50) of Galleria mellonella infected by B. bassiana. This delay could be attributed to reduced Pr1 proteases and extracellular cuticle-degrading enzymes, diminished dimorphic transition rates, and dysregulated antioxidant enzymes. Additionally, the absence of Rad6 had a more pronounced effect on genetic information processing, metabolism, and cellular processes under normal conditions. However, its impact was limited to metabolism in oxidative stress. This study offers a comprehensive understanding of the pivotal roles of Rad6 in conidial and hyphal stress tolerance, environmental adaptation, and the pathogenesis of Beauveria bassiana.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and transmission of pretreatment drug resistance in people living with HIV-1 in Shanghai China, 2017-2021. 2017-2021年中国上海HIV-1感染者预处理耐药性的流行和传播情况。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-27 DOI: 10.1080/21505594.2024.2373105

Min Zhang, Yingying Ma, Zhenyan Wang, Gang Wang, Qianying Wang, Xin Li, Feng Lin, Chiyu Zhang

{"title":"Prevalence and transmission of pretreatment drug resistance in people living with HIV-1 in Shanghai China, 2017-2021.","authors":"Min Zhang, Yingying Ma, Zhenyan Wang, Gang Wang, Qianying Wang, Xin Li, Feng Lin, Chiyu Zhang","doi":"10.1080/21505594.2024.2373105","DOIUrl":"10.1080/21505594.2024.2373105","url":null,"abstract":"The implementation of pretreatment drug-resistance (PDR) surveillance among people living with HIV-1 (PLWH) is a top priority in countries using efavirenz (EFV)/nevirapine (NVP) for first-line ART. In this study, we assessed the prevalence of PDR among PLWH in Shanghai, China during 2017-2021, and to reveal PDR transmission between Shanghai and other regions of China. A total of 5050 PLWH not on ART during 2017-2021 were included. Partial HIV-1 pol sequences were amplified, sequenced, and analysed for drug-resistance mutations (DRMs). Besides, transmission network of PDR variants was inferred using HIV-TRACE. The overall prevalence of PDR was 4.8% (242/5050; 95% CI, 4.2-5.4). Prevalence of NNRTI-associated PDR was 3.9% (95% CI, 3.4-4.5), higher than those of NRTI-associated (0.8%; 95% CI, 0.5-1.1) and PI-associated PDR (0.9%; 95% CI, 0.6-1.2). High prevalence of PDR (especially high-level resistance) to EFV (132/5050, 2.6%) and NVP (137/5050, 2.7%) were found. CRF01_AE (46.0%) was the predominant HIV-1 genotype with any DRMs, followed by CRF55_01B (21.0%), and CRF07_BC (15.1%). Two NRTI-associated (S68G/N/R and T215A/N/S/Y), five NNRTI-associated (V179D/E/T/L, K103N/R/S/T, E138A/G/K, V106M/I/A and Y181C/I) and two PI-associated mutations (M46I/L/V and Q58E) were the most common observed DRMs in PDR patients in Shanghai. The vast majority of S68G occurred in CRF01_AE (45%). M46I/L/V and Q58E showed a relatively high prevalence in CRF01_AE (4.1%) and CRF07_BC (12.6%). Transmission network analyses demonstrated cross-regional transmission links of PDR variants between Shanghai and other regions of China, which was mainly driven by the potential low-level DRM V179D/E. These results provide crucial information for clinical decision making of first-line ART in PLWH with PDR.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11212556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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