Veterinary parasitology最新文献

{"title":"Pharmacological evaluation on benzimidazole anthelmintics for eradication of the gill fluke Microcotyle sebastis infesting a black rockfish, Sebastes schlegelii","authors":"Chan Young Yang ,&nbsp;Jun Sung Bae ,&nbsp;Ga Won Kim ,&nbsp;Chae Won Lee ,&nbsp;Eun Ha Jeong ,&nbsp;Mun-Gyeong Kwon ,&nbsp;Jung Soo Seo ,&nbsp;Chan-Il Park ,&nbsp;Ji-Hoon Lee","doi":"10.1016/j.vetpar.2025.110573","DOIUrl":"10.1016/j.vetpar.2025.110573","url":null,"abstract":"<div><div>This study comprehensively evaluated the <em>in vitro</em> and <em>in vivo</em> anthelmintic efficacy and pharmacokinetics of four benzimidazole compounds: febantel (FB), fenbendazole (FBZ), albendazole (ABZ), and mebendazole (MBZ) against the gill fluke <em>Microcotyle sebastis</em> in cultured black rockfish (<em>Sebastes schlegelii</em>). <em>In vitro</em> tests with isolated adult parasites demonstrated that ABZ exhibited the highest efficacy, followed by FBZ, while MBZ showed minimal activity and FB remained largely inactive at lower concentrations. At the highest concentration tested (200 mg/L), FB showed only weak, nonspecific activity. In <em>in vivo</em> experiments, ABZ was the most effective drug when orally administered at 50 mg/kg for three consecutive days, while FB demonstrated greater efficacy than FBZ, and MBZ exhibited the lowest efficacy under the tested conditions. Pharmacokinetic analysis revealed marked variation among the drugs, with FB achieving the highest active metabolite levels at 100 mg/kg. ABZ, at 50 mg/kg, achieved comparable systemic exposure to FBZ at 100 mg/kg through its active metabolite albendazole sulfoxide (ABZSO). These findings highlight ABZ as the most efficacious compound, with FB showing promise through metabolic conversion to FBZ, and MBZ demonstrating limited efficacy due to poor absorption and rapid inactivation. Overall, this study provides an integrated evaluation of both efficacy and pharmacokinetics, indicating that ABZ and FB are effective candidates for managing <em>M. sebastis</em> infections in aquaculture.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110573"},"PeriodicalIF":2.2,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144810329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of β-tubulin isotypes in Uncinaria stenocephala and implications for benzimidazole resistance in hookworms","authors":"Thomas Stocker ,&nbsp;Jan Šlapeta","doi":"10.1016/j.vetpar.2025.110569","DOIUrl":"10.1016/j.vetpar.2025.110569","url":null,"abstract":"<div><div><em>Uncinaria stenocephala</em> is a widespread hookworm of dogs across Europe, Canada, southern Australia, and other temperate regions, where it often outnumbers infections caused by <em>Ancylostoma caninum</em>. Although a putative β-tubulin isotype-1 mutation associated with resistance has been detected in <em>U. stenocephala</em>, clinical resistance to benzimidazoles has not yet been confirmed. Benzimidazole resistance is primarily linked to single nucleotide polymorphisms (SNPs) in the β-tubulin isotype-1 gene; however, the β-tubulin genes of <em>U. stenocephala</em> have not been fully characterised. We aimed to identify β-tubulin genes and confirm the coding sequences for key residues (Q134, F167, E198, and F200) in the β-tubulin isotype-1 gene of the <em>U. stenocephala</em> genome. Two <em>U. stenocephala</em> specimens were subjected to Illumina sequencing, and species identity was confirmed through morphological and molecular analysis using ITS rDNA and <em>cox</em>1 markers. Genome assembly revealed the presence of β-tubulin isotype-1 (10 exons) and isotype-2 (9 exons), both homologous to β-tubulins from other hookworms (<em>A. caninum</em>, <em>A. ceylanicum</em>, <em>A. duodenale</em> and <em>Necator americanus</em>). The β-tubulin isotype-1 protein sequence of <em>U. stenocephala</em> contained two variable residues (S37Q and G441A) compared to other hookworm sequences. While the isotype-2 protein sequence was conserved among <em>Ancylostoma</em> species, <em>U. stenocephala</em> exhibited six distinct polymorphisms (E39D, T40S, N115S, L130I, A287S, T439G). The benzimidazole-susceptible residues (Q134, F167, E198, F200) were present in the β-tubulin isotype-1 protein sequence. Characterisation of the complete coding regions of β-tubulin isotypes 1 and 2 enables population-level screening for benzimidazole resistance-associated SNPs and provides a foundation for future epidemiological studies in <em>U. stenocephala</em>.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110569"},"PeriodicalIF":2.2,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative diagnostic strategies for Taenia ovis infection: Exploring molecular, gene expression, oxidative stress, and histopathological insights","authors":"Mai A. Salem ,&nbsp;Marwa M. Khalifa ,&nbsp;Noha M. Bakry ,&nbsp;Mohamed S. Kamel ,&nbsp;Mawada Mohamed Ali ,&nbsp;Ehab A. Fouad ,&nbsp;Reem M. Ramadan","doi":"10.1016/j.vetpar.2025.110567","DOIUrl":"10.1016/j.vetpar.2025.110567","url":null,"abstract":"<div><div><em>Taenia ovis</em> is an economically significant cestode affecting sheep. Its larval stage, <em>Cysticercus ovis,</em> causes ovine cysticercosis, leading to substantial economic losses due to carcass condemnation and reduced meat quality. This study utilized a comprehensive approach to investigate the prevalence, phylogenetic characterization, immune response, oxidative stress markers, and histopathological changes associated with <em>T. ovis</em> infection in naturally infected sheep. Among 220 slaughtered sheep examined post-mortem, an overall prevalence of 18.63 % was observed, with infection rates higher in males (20.83 %) than in females (16 %). Seasonal variation demonstrated the highest prevalence in spring (41.46 %), followed by winter (29.27 %), autumn (17.07 %), and summer (12.20 %). Molecular characterization of <em>T. ovis</em> isolates, which is based on the mitochondrial cytochrome c oxidase subunit I gene, revealed a high genetic similarity between Egyptian isolates and those from New Zealand, indicating genetic stability across geographically distinct regions. Immune response profiling showed significant upregulation (P &lt; 0.0001) of cytokines such as interleukin 1β, interleukin 10, transforming growth factor β, interleukin 5, toll-like receptor 7, and gata-binding protein 3, indicating a robust inflammatory and immune-modulatory response. Further analysis of oxidative stress markers revealed marked increases in malondialdehyde and total oxidant status, reflecting heightened oxidative damage, while elevated total antioxidant capacity suggested a compensatory antioxidant response. Histopathological examination confirmed the infection through the observation of granulomatous inflammation, fibrosis, myositis, and calcification in the affected tissues. These findings underscore the pathogenic impact of <em>T. ovis</em> and provide valuable insights into its biology, emphasizing the need for improved diagnostic and control strategies to mitigate its economic and agricultural burdens.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110567"},"PeriodicalIF":2.2,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shifting Paradigms: Imidocarb dipropionate as an alternative chemotherapeutic strategy for Trypanosoma evansi infection in animals","authors":"Snehil Gupta ,&nbsp;Khushboo Sethi ,&nbsp;Sukhdeep Vohra ,&nbsp;Surbhi Gupta ,&nbsp;Ruma Rani ,&nbsp;Sanjay Kumar ,&nbsp;Rajender Kumar","doi":"10.1016/j.vetpar.2025.110558","DOIUrl":"10.1016/j.vetpar.2025.110558","url":null,"abstract":"<div><div>We report on the <em>in vitro</em> and <em>in vivo</em> success of Imidocarb dipropionate (IDP), an aromatic diamidine compound, as an effective drug against Surra-causing organism, <em>Trypanosoma evansi</em>. IDP is a clinically acclaimed drug for the treatment of babesiosis and anaplasmosis. We have also investigated cytotoxic effect of IDP on equine Peripheral mononuclear cells (PBMCs) and Vero cell line. The data generated indicate that the IC₅₀ of IDP (2.73 µM) was significantly lower than its cytotoxic concentration (32.66 and 80.70 µM against PBMCs and Vero cell line, respectively). We have recorded significant downregulation in mRNA expression of genes responsible for cellular regulatory activities such as cell division, glycolysis, nucleic acid synthesis, immune evasion strategy and redox homeostasis. During mice experimentation, dose of 20 mg/kg body weight was found to prolong the survival period of <em>T. evansi</em> infected mice by 20 days following two subsequent intra-peritoneal injections administered 72 h apart. Hematological, biochemical and histopathological investigations showed the effect of IDP on mice kidney and liver at four times multiples of the therapeutic dosage. Further, clinical trials on higher-order organisms are required before recommendation of IDP against <em>T. evansi</em> in the clinical host.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"338 ","pages":"Article 110558"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The nanoparticulate lipid secretome in the lifecycle of Oesophagostomum dentatum: Shedding light on the role in parasitic development","authors":"Anna Sophia Feix ,&nbsp;Bärbel Ruttkowski ,&nbsp;Silvio Kau-Strebinger ,&nbsp;Anja Joachim","doi":"10.1016/j.vetpar.2025.110556","DOIUrl":"10.1016/j.vetpar.2025.110556","url":null,"abstract":"<div><div>The parasitic nematode <em>Oesophagostomum dentatum</em>, a member of the order Strongylida, commonly infects pigs worldwide. Its secretome includes proteins, bioactive lipids, and extracellular particles shed in changing composition during development. However, the lipid components of the secretome, including their stage-specific variations and functional significance, remain poorly understood. This study aimed to analyze lipid-containing particles shed by larval and adult stages, and to characterize alterations in lipidomic profiles across the developmental stages. Extracellular nanoparticles (NPs) shed by the parasites, representing a less explored component of the secretome, were isolated using ultracentrifugation and subjected to NP tracking. For a detailed lipidomic and metabolomic analysis of the secretome of different <em>O. dentatum</em> stages, FTIR and LC-MS/MS were applied. The data were compared across life cycle stages to identify developmental and functional variations. The lipidomic analysis revealed distinct profiles for larval and adult stages, with stage-specific lipid classes and metabolites. Shed particles were found to carry a diverse array of lipids, including phospholipids, sphingolipids, and sterols, as well as fatty acids that may be key for interactions with the host, including immune evasion. Comparative analyses demonstrated developmental shifts in lipid composition, highlighting possible adaptations to larval migration, nodule formation, and adult reproduction. This study provides the first comprehensive lipidomic characterization of <em>O. dentatum</em> as a model for gastrointestinal nematodes, unveiling significant stage-specific lipidomic and metabolic adaptations. The detailed analysis of shed nanoparticles as part of the secretome offers new insights into the biology of parasitic nematodes and their interactions with the host environment.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"338 ","pages":"Article 110556"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal detection of Sarcocystis aucheniae DNA in blood samples of naturally infected alpacas in the Andean highlands of Peru","authors":"Luisa Neyra ,&nbsp;Jose L. Malaga ,&nbsp;Susana Giuliano ,&nbsp;Ximena Barriga Marcapura ,&nbsp;Milagros Aguilar ,&nbsp;Miguel Angel Chavez Fumagalli ,&nbsp;Monica Florin-Christensen ,&nbsp;Juan Reategui Ordoñez","doi":"10.1016/j.vetpar.2025.110555","DOIUrl":"10.1016/j.vetpar.2025.110555","url":null,"abstract":"<div><div><em>Sarcocystis aucheniae</em> is a protozoan parasite that forms macroscopic cysts in the skeletal muscles of South American camelids (SAC), impairing meat commercialization and posing a significant barrier to sustainable camelid livestock development. Although <em>S. aucheniae</em> DNA has been previously detected in SAC blood, the temporal dynamics of parasitemia remain unclear. This study presents the first longitudinal analysis of <em>S. aucheniae</em> DNA detection in alpaca (<em>Vicugna pacos</em>) peripheral blood. Monthly blood samples were collected from 38 male alpacas over 10 months in a SAC breeding establishment in Yanque, Peru. A duplex semi-nested PCR was used to detect DNA from parasites and the host, the latter as an extraction control. <em>Sarcocystis aucheniae</em> was detected in 9 animals (24 %) during the study. The maximum time length of positive detection was 2 months in 2 alpacas, while the rest of the animals tested positive in only one sampling. This suggests a brief window of blood-circulating stages, before encysting in the muscles. In one alpaca, positive and negative detection alternated in consecutive months, which might indicate fluctuating parasitemia levels, or re-infection. No correlation was found between PCR positivity and host age, temperature, or precipitation. Primer specificity was assessed by <em>in silico</em> alignments, and analytical sensitivity was estimated at 0.46 pg/μl of DNA. Although the method has limited diagnostic value in chronic infections, it is useful for epidemiological surveillance and transmission monitoring. This study provides novel insights into the infection kinetics of <em>S. aucheniae</em> and highlights the value of molecular tools in understanding parasitic transmission in SAC.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"338 ","pages":"Article 110555"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation of carvacrol's anticoccidial activity: Integrating in vitro, in vivo, and in silico approaches using carvacrol-treated Eimeria tenella oocysts","authors":"Esraa A. Ahmed ,&nbsp;Shawky M. Aboelhadid ,&nbsp;Almahy M. El-Mallah ,&nbsp;Saleh Al-Quraishy ,&nbsp;Piotr Falkowski ,&nbsp;Abdel-Razik H. Abdel-Razik ,&nbsp;Abdel-Azeem S. Abdel-Baki","doi":"10.1016/j.vetpar.2025.110560","DOIUrl":"10.1016/j.vetpar.2025.110560","url":null,"abstract":"<div><div>The present study evaluated the <em>in vitro</em> oocysticidal activity of carvacrol against <em>Eimeria tenella</em>, assessed its pathogenicity in experimentally infected chicks, and explored its potential anticoccidial mechanisms through <em>in silico</em> analysis. <em>In vitro</em> assays demonstrated that carvacrol exhibited substantial efficacy, with 89.03 % inhibition of unsporulated oocyst sporulation and 85.5 % destruction of sporulated oocysts at a 10 % concentration. Oxidative stress markers in untreated oocysts showed elevated levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx), alongside reduced malondialdehyde (MDA) levels, indicating an oxidative stress resistance likely essential for oocyst survival. The calculated LC₅₀ and LC₉₀ values of carvacrol against sporulated oocysts were 3.97 % and 10.82 %, respectively. For the <em>in vivo</em> evaluation, 60 chicks were divided into three groups (n = 20 per group). Birds were orally inoculated with 1 × 10⁴ oocysts previously treated with either the LC₅₀ or LC₉₀ concentration of carvacrol, while the control group received untreated oocysts. Chicks infected with carvacrol-treated oocysts exhibited significant reductions in bloody diarrhea, intestinal lesion scores, and oocyst shedding compared to the untreated control group. Histopathological examination revealed fewer developmental stages of <em>E. tenella</em> and minimal damage to the intestinal glands in treated groups, corroborating the anticoccidial efficacy of carvacrol. Biochemical analysis of cecal tissue showed that the untreated control group maintained higher SOD and GPx activity and lower MDA levels than the carvacrol-treated groups, further indicating a disruption of the parasite’s oxidative stress defenses. Additionally, molecular docking studies demonstrated strong binding affinities between carvacrol and key <em>E. tenella</em> proteins, including fructose-1,6-bisphosphate aldolase (ALD), cyclin-dependent kinase (CDK), and apical membrane antigen 1 (AMA1), suggesting a possible mechanism through which carvacrol interferes with parasite survival and replication. Overall, these findings position carvacrol as a promising natural anticoccidial compound, exerting both direct oocysticidal effects and molecular interference with essential parasitic proteins, thereby offering a viable natural alternative to conventional synthetic drugs for the control of avian coccidiosis.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110560"},"PeriodicalIF":2.2,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiparasitics against ectoparasites in small animals– important pharmaceutical substances or underestimated environmental hazards?","authors":"Anja Joachim ,&nbsp;Lucy J. Robertson ,&nbsp;Ezio Ferroglio ,&nbsp;Wolfgang Bäumer ,&nbsp;Michael Leschnik","doi":"10.1016/j.vetpar.2025.110557","DOIUrl":"10.1016/j.vetpar.2025.110557","url":null,"abstract":"<div><div>Application of chemical compounds for the control and prevention of arthropod infestations is standard in small animal veterinary medicine. However, concerns about potential negative effects of such substances in the environment, including their circulation in water, soil or plants, and consequent impacts, including infertility or death of sensitive non-target organisms, such as bird, fish, and insects, in particular pollinators, are increasing. Factors that determine the risk of environmental harm from different chemicals are not only their release, but also their retention, bioavailability, degradation, accumulation in different environmental compartments, bioaccumulation in different organisms or their organs, and the effects of excipients commonly used in drug formulations vs. not medical or veterinary use of chemicals. Here we briefly review the substance classes of insecticides, acaricides, or repellents used in veterinary medicine, their effects and possible side effects, and their fate in the environment, including reports of undesirable environmental impacts. In addition to existing literature, the possibilities of preventing, reducing and containing the unwanted release of such chemicals from animal treatments and the pivotal role of experts in veterinary parasitology are discussed. Knowledge gaps concerning the properties of chemicals used for the control of ectoparasites in veterinary medicine that must be addressed in future research are emphasized.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110557"},"PeriodicalIF":2.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interferon alfa and beta receptor confers resistance against Neospora caninum infection in mice","authors":"Vanessa dos Santos Miranda,&nbsp;Flávia Batista Ferreira,&nbsp;Mariana Ferreira Silva,&nbsp;Vanessa Resende Souza Silva,&nbsp;Fernando William Moreira Santana,&nbsp;José Roberto Mineo,&nbsp;Tiago Wilson Patriarca Mineo","doi":"10.1016/j.vetpar.2025.110559","DOIUrl":"10.1016/j.vetpar.2025.110559","url":null,"abstract":"<div><div><em>Neospora caninum</em> is an intracellular protozoan that is the causative agent of neosporosis, a disease relevant to the veterinary field for causing neuromuscular disease in dogs and repeated abortions in ruminants. In the present moment, there are no therapies that are able to induce sterile cure against the infection and prevention strategies rely only in environmental management. In this context, we aimed to determine the protective role of the Interferon alpha and beta receptor (IFNAR) during an experimental infection with <em>N. caninum</em>. We performed <em>in vivo</em> experiments with genetically deficient mice (<em>Ifnar</em>^-/-). Those mice, in direct comparison with wildtype (WT) controls, presented reduced survival against the infection, associated with increased parasite load and inflammation in different tissues. In agreement, we observed that the absence of the IFN-I receptor led to a significant reduction in crucial inflammatory mediators that control the infection, namely IL-12, IFN-γ and nitric oxide. Together, our results showed that IFNAR is a central molecule that mediates host resistance against <em>N. caninum</em> by controlling acute and chronic parasite replication, through the induction of an effective Th1 immune response against this protozoan parasite<em>.</em></div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110559"},"PeriodicalIF":2.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural characterization of bioaccumulation and migration of Ag nanoparticles in host-parasite organisms","authors":"Fuad Rzayev ,&nbsp;Eldar Gasimov ,&nbsp;Ali Nasirov ,&nbsp;Sarvinaz Hajiyeva ,&nbsp;Mehri Seyidbeyli ,&nbsp;Aladdin Eyvazov ,&nbsp;Gunay Rzayeva","doi":"10.1016/j.vetpar.2025.110554","DOIUrl":"10.1016/j.vetpar.2025.110554","url":null,"abstract":"<div><div>In an era of developing nanotechnologies, studying the bioaccumulation and migration of nanoparticles in various components of the ecosystem, and the varying degrees of pathology they cause in living organisms - is important. In the present study, the bioaccumulation and migration of nanoparticles in both the host and parasite were examined by light and electron microscopy, focusing on the nematode <em>Heterakis dispar</em>, which causes serious damage to the organism of the domestic goose. Silver nanoparticles (Ag NPs) were administered to birds infected with <em>H. dispar</em> at a concentration of 100 μg/ml (total volume 10 ml). The parasites, as well as intestine, liver, and skeletal striated muscle of the host, were examined by histological methods and electron microscopy. It was found that the sizes of AgNPs at the free state were ranging from 9.03 to 23.82 nm (13.88 ± 0.48 nm), while in the parasite organism they were up to 14 nm, and in birds they did not exceed 13 nm. Nanoparticles bioaccumulated in the parasite, causing pathological changes. AgNPs were observed to migrate through the integumentary tissue of the parasite into the pseudocoelomic cavity organs. Various pathological changes occurred in the structural elements of the intestine, liver, and skeletal striated muscle of birds due to the action of AgNPs. Nanoparticles entered the cytoplasm of erythrocytes located in the lumen of the vessels in the submucosal layer of the goose intestine and subsequently migrated to the liver and striated muscle.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110554"},"PeriodicalIF":2.2,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}