Veterinary parasitology最新文献

{"title":"Male mites are the promising targets for control of Dermanyssus gallinae (Acari: Dermanyssidae) based on the reproductive biology research","authors":"Boxing Liu,&nbsp;Jiaqi He,&nbsp;Qi Liu,&nbsp;Bohan Wang,&nbsp;Mingjun Xiong,&nbsp;Weiwei Sun,&nbsp;Baoliang Pan","doi":"10.1016/j.vetpar.2025.110411","DOIUrl":"10.1016/j.vetpar.2025.110411","url":null,"abstract":"<div><div><em>Dermanyssus gallinae</em> is a significant hematophagous ectoparasite affecting laying hens. However, due to the limitations of research methods, especially feeding methods, the reproductive biology of <em>D. gallinae</em> has not been fully studied. In this study, two recently developed <em>in vivo</em> feeding methods were employed to investigate the reproductive biology of <em>D. gallinae</em>, including the impacts of mating on feeding status and egg laying, the reproductive capacity of female mites, the mating capacity of male mites and the sex ratio of offspring. The results demonstrated that only mated adult female mites were able to reach an engorged state and were capable of oviposition. The maximum egg-laying times for an adult female mite was 13, with an average of 44.39 eggs per mite. Moreover, for the first time, this study described the mating ability of adult male mites. On average, a male mite can mate with 16 female mites during its lifetime and the average mating period is 8.47 d. All offspring from the first batch of eggs produced by novel adult female mites were males. Afterward, the proportion of male offspring mites gradually decreases with the generation. The results show that the males play a crucial role in the population establishment of <em>D. gallinae</em>, indicating they can be considered promising targets for the mite control.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110411"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and risk factors of equine piroplasmosis in Portugal: A five-year retrospective study","authors":"Ana Cabete ,&nbsp;Ângela Xufre ,&nbsp;Ludovina Padre ,&nbsp;Elisa Bettencourt ,&nbsp;Telmo Nunes ,&nbsp;Jacinto Gomes","doi":"10.1016/j.vetpar.2024.110378","DOIUrl":"10.1016/j.vetpar.2024.110378","url":null,"abstract":"<div><div>Equine piroplasmosis (EP) is a tick-borne disease of equids caused by <em>Theileria equi</em>, <em>Theileria haneyi,</em> and <em>Babesia caballi.</em> EP is endemic in most tropical and subtropical regions worldwide, and there is a likelihood that it is also endemic in Portugal. This retrospective study aimed to determine the seroprevalence, prevalence, and potential risk factors of EP in our country over the past five years. A total of 3063 diagnostic test records were analysed. Results from the competitive enzyme-linked immunosorbent assay (cELISA) revealed a seroprevalence of 32.7 % and 15.7 % for <em>T. equi</em> and <em>B. caballi</em>, respectively, with a coinfection rate of 7.4 %. For the indirect fluorescent antibody test (IFAT), 38.8 % of the samples were positive for <em>T. equi</em>, 45.7 % for <em>B. caballi,</em> and 23.1 % for both parasites. Prevalence determined using quantitative polymerase chain reaction (qPCR) showed 40.5 % <em>T. equi-</em>positive cases, 8.3 % <em>B. caballi</em>-positive cases, and 3.2 % mixed infections in the studied population. Considering risk factors, age and season appear to be associated with higher seropositivity, and location was also found to play a significant role. This study represents the first retrospective analysis carried out in Portugal, confirming the endemicity of EP in the country. Further studies are needed to corroborate our findings, to determine actual prevalence and seroprevalence in the Portuguese general equine population, and to identify risk factors better, helping breeders and owners to minimise the health and economic impact of EP.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110378"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatozoon infections in dogs and cats in USA: History, life cycle, and suggestions for uniform terminology for hepatozoonosis in endotherms and ectotherms","authors":"J.P. Dubey ,&nbsp;Gad Baneth","doi":"10.1016/j.vetpar.2025.110408","DOIUrl":"10.1016/j.vetpar.2025.110408","url":null,"abstract":"<div><div>Hepatozoonosis can cause serious illness in dogs in the USA resulting in muscle weakness and bone deformities. It is often fatal, and treatment is expensive and not curative. There are two recognized species of <em>Hepatozoon</em> which infect dogs, <em>Hepatozoon canis</em> and <em>Hepatozoon americanum</em>, transmitted by ticks, <em>Rhipicephalus sanguineus</em> sensu lato and <em>Amyboloma maculatum</em>, respectively. <em>Hepatozoon canis</em> has worldwide distribution and <em>H. americanum</em> is currently confined to the USA. Wildlife reservoirs of <em>H. americanum</em> have not been established but coyote (<em>Canis latrans</em>) is suspected, and infection is common. Most cases of hepatozoonosis in dogs in USA are thought to be due to <em>H. americanum</em>. However, molecular evidence suggests that a minor portion of cases in dogs are due to <em>H. canis</em>, sometimes as coinfection. Hepatozoonosis in domestic and wild felids, caused by <em>Hepatozoon felis</em> and <em>Hepatozoon silvestris</em>, but also <em>H. canis</em>, has been reported from several countries but little is known of its occurrence in domestic cats or wild felids in the USA. Here, life cycle stages of <em>Hespatozoon</em> spp. in dogs are compared and illustrated. Recently, a new species of <em>Hepatozoon</em>, <em>Hepatozoon rufi,</em> was described from bobcats in Mississippi, USA. There is considerable confusion concerning the terminology for different developmental stages in the life cycle of mammalian <em>Hepatozoon</em> spp. Here, the authors review hepatozoonosis in dogs and cats in the USA and propose a uniform terminology for the life cycle stages.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110408"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143307751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nematophagous fungi to controlling gastrointestinal nematodes in small ruminants: A systematic review","authors":"Luis Eduardo Meira Faria ,&nbsp;Júlia dos Santos Fonseca ,&nbsp;Jackson Victor de Araújo ,&nbsp;Lorendane Millena de Carvalho ,&nbsp;George Rego Albuquerque ,&nbsp;Wendell Marcelo de Souza Perinotto","doi":"10.1016/j.vetpar.2025.110410","DOIUrl":"10.1016/j.vetpar.2025.110410","url":null,"abstract":"<div><div>Gastrointestinal nematode infections are responsible for production losses in small ruminant worldwide. Moreover, anthelmintic resistance has increased mortality. New sustainable control strategies have been developed to overcome this problem. Biological control has emerged as a new trend with the recent market introduction of bioproducts. This systematic review aimed to analyze publications on the production of formulations and bioproducts based on helminthophagous fungi used to control gastrointestinal nematodes in sheep and goats, and to survey patents filed with this recommendation. The guidelines PRISMA 2020 were used, searching from 2004 to 2024. The following scientific databases were used: Springer, Science Direct, SciELO, Web of Science, Google Scholar, CAPES Journals and PubMed. The search for patents was carried out using the PATENTSCOPE database. Duplicate articles; review/opinion articles; studies in other animal species; studies without approval by an ethics committee; and tests performed only under in vitro conditions were excluded. Nine studies were included, six of which tested formulations and three tested bioproducts. <em>Duddingtonia flagrans</em> was most frequently included (8), being found in the two available bioproducts. Brazil shows the largest number of studies in the area (5). Sheep were more frequent (7) than those with goats (2). Reduction percentages ranged from 6 % to 96.6 %. Regarding patent searches, only three were found, two of which were for <em>D. flagrans</em> formulations and one for a utility model with <em>Arthrobotrys drechslen</em>. <em>D. flagrans</em> is the nematophagous fungus with the greatest potential to produce bioproducts in the biological control of gastrointestinal nematodes in small ruminants.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110410"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of thymoquinone analogues with high anthelmintic activity against monogenean infections in goldfish (Carassius auratus)","authors":"Xiao Tu ,&nbsp;Jiaxin Hu ,&nbsp;Jinghao Peng ,&nbsp;Qihan Chen ,&nbsp;Yangle Zhao ,&nbsp;Zemao Gu","doi":"10.1016/j.vetpar.2025.110401","DOIUrl":"10.1016/j.vetpar.2025.110401","url":null,"abstract":"<div><div>Monogenean parasites are harmful pathogens in aquaculture systems. Current treatment strategies for monogenean infections are unsatisfactory, making the discovery of new drugs urgent. Thymoquinone (TQ), a natural monoterpene isolated from <em>Nigella sativa</em> L., has demonstrated its potential as lead structure against the monogenean parasites. In the present study, the anthelmintic activity of 26 selected TQ analogues was investigated against the monogenean parasite <em>Gyrodactylus kobayashii</em> in the goldfish (<em>Carassius auratus</em>). The results indicated that 18 TQ analogues displayed the EC₅₀ value below 1.0 mg/L, 14 of them showed more potent anthelmintic activity than TQ (EC₅₀=0.303 mg/L). Particularly, compounds <strong>9</strong> and <strong>23</strong> exhibited the best efficacies with the EC₅₀ values of 0.058 and 0.034 mg/L, outperforming the standard drug praziquantel as well. Structure-activity relationship analysis revealed that large electron-donating substituents at the 2-position of the quinone ring contribute to enhanced <em>in vivo</em> anthelmintic activity. Additionally, four analogues (<strong>9</strong>, <strong>12</strong>, <strong>23</strong> and <strong>25</strong>) displayed low fish toxicity, with the therapeutic index (TI, LC₅₀/EC₅₀) above 9.23, and were selected for further <em>in silico</em> drug‑likeness assessment. The results demonstrated an excellent drug-likeness profile and an adherence to major pharmaceutical companies’ filters for compounds <strong>9</strong> and <strong>23</strong>. Besides, TQ analogue <strong>9</strong> was chosen for an <em>in vivo</em> study in large-scale trial based on its potency, low fish toxicity, and excellent drug-likeness profiles. Treatment of <em>G. kobayashii</em> infected goldfish for 24 h at 0.1 and 0.2 mg/L was effective, reducing the infection prevalence from 100 % to 0 % post-treatment. The experimental fish exhibited normal behavior, despite minor modifications were displayed in the gills and liver according to histopathological results. Collectively, this study suggested that TQ analogues are excellent scaffolds in the discovery of novel anthelmintic agents against monogenean infections.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110401"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leishmania sp. can affect the cardiovascular system of dogs – A systematic review of over 80 years","authors":"Felipe Gaia de Sousa ,&nbsp;Fabiana Silva Fádel Queiroz ,&nbsp;Roberto Baracat de Araújo ,&nbsp;Suzane Lilian Beier","doi":"10.1016/j.vetpar.2024.110388","DOIUrl":"10.1016/j.vetpar.2024.110388","url":null,"abstract":"<div><div>Cardiovascular involvement in <em>Leishmania</em> sp. infections still requires further elucidation, with cutaneous and organic changes being more frequently observed. The aim of this study was to conduct a systematic review of cardiovascular involvement in dogs with canine visceral leishmaniasis, considering a publication span of over 80 years. A bibliographic search focused on canine visceral leishmaniasis and cardiovascular involvement via the descriptors “leishmaniasis,” “heart,” and “dogs” from 1940 to 2024. An analysis of over 80 years of published documents was performed across four databases: PubMed, Web of Science, Scopus, and Portal Capes. The inclusion and exclusion criteria were predetermined to ensure proper triage, considering documents that described cardiovascular involvement in canine visceral leishmaniasis. Initially, 250 documents were identified via the specified descriptors. After applying the inclusion and exclusion criteria, 29 articles were deemed eligible. Most studies were dated from 2005 to 2024. Histopathological analysis of heart samples was performed in nearly 20 studies. The animals evaluated were over 6 months old, with <em>Leishmania infantum</em> being the most frequently described species. Cardiovascular alterations were observed from electrophysiological, echocardiographic, and histopathological/immunohistochemical perspectives and in biomarker concentrations. Canine visceral leishmaniasis has extensive dissemination potential, emphasizing the need for veterinary and epidemiological surveillance services to monitor the spatial/temporal patterns of this disease. Cases of myocarditis with inflammatory infiltrates were documented in all reviewed studies. Routine cardiovascular evaluation should be integrated into the assessment and monitoring of canine visceral leishmaniasis, considering clinical, laboratory, and imaging evidence of cardiovascular alterations.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110388"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143182788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Duddingtonia flagrans spores fed in trace mineral mix to lambs in reducing the development of gastrointestinal nematode larvae in feces","authors":"Joan M. Burke ,&nbsp;Suman Rohila ,&nbsp;Elisa Preston ,&nbsp;Cathleen C. Williams ,&nbsp;Clare M. Scully ,&nbsp;Brooke A. Delcambre ,&nbsp;Katherine H. Petersson ,&nbsp;Elizabeth Kass ,&nbsp;Mohan Acharya ,&nbsp;James E. Miller ,&nbsp;Adriano F. Vatta","doi":"10.1016/j.vetpar.2025.110414","DOIUrl":"10.1016/j.vetpar.2025.110414","url":null,"abstract":"<div><div>Providing spores of the nematode-trapping fungus, <em>Duddingtonia flagrans</em>, in the diet of livestock leads to fewer gastrointestinal nematode (<strong>GIN</strong>) larvae on pasture. The objective was to determine the efficacy of <em>D. flagrans</em> (<strong>Df</strong>; BioWorma®) fed in a mineral mix compared with a feed supplement to naturally infected lambs (predominantly <em>Haemonchus contortus</em> and <em>Trichostrongylus/Teladorsagia</em> spp.) to reduce the number of GIN larvae cultured. Initially, the efficacy of Df spores was examined in a preliminary experiment in which lambs were fed a control supplement without Df (<strong>CON</strong>) or with Df (n = 8/treatment) for 7 days. Feces were collected every other day for three coprocultures. Feeding Df led to a 73 % reduction in stongylid third-stage larvae (<em>P</em> = 0.005). In the second experiment, weaned lambs were fed a 12 % crude protein grain supplement with trace mineral added without Df (CON), Df mixed in feed (<strong>DfC</strong>) or in trace mineral (<strong>DfM</strong>; n = 8/treatment) for 16 days. Strongylid larval recovery was reduced in both Df groups (<em>P</em> &lt; 0.001), and the third-stage larval recovery was lower in DfM than DfC (<em>P</em> = 0.001). In a field study, similar treatments were used (n = 16/treatment including 2 reps/treatment) using weaned lambs for a 30-day period. The sheep were fed a 16 % crude protein commercial grain mix and a commercial mineral mix; for the DfM group, Df spores were mixed with the mineral mix 7 days prior to feeding. Similarly, strongylid larval recovery was reduced in both Df groups (<em>P</em> &lt; 0.001) but was similar between Df treatments (<em>P</em> = 0.62). No treatment effect on <em>Strongyloides</em> larvae recovered was noted (<em>P</em> = 0.181). Including Df in a trace mineral mix offers promise in control of GIN on pasture or range for flocks that do not offer grain supplements.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110414"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143307752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of deep learning-based mobile application for the identification of Coccidia species in pigs using microscopic images","authors":"Naseeb Singh ,&nbsp;Vijay Mahore ,&nbsp;Meena Das ,&nbsp;Simardeep Kaur ,&nbsp;Surabhi Basumatary ,&nbsp;Naphi Roi Shadap","doi":"10.1016/j.vetpar.2024.110376","DOIUrl":"10.1016/j.vetpar.2024.110376","url":null,"abstract":"<div><div>Coccidiosis is a gastrointestinal parasitic disease caused by different species of <em>Eimeria</em> and <em>Isospora</em>, poses a significant threat to pig farming, leading to substantial economic losses attributed to reduced growth rates, poor feed conversion, increased mortality rates, and the expense of treatment. Traditional methods for identifying Coccidia species in pigs rely on fecal examination and microscopic analysis, necessitating expert personnel for accurate species identification. To address this need, a deep learning-based mobile application capable of automatically identifying different species of <em>Eimeria</em> and <em>Isospora</em> was developed. The present study focused on six species, namely, <em>E</em>. <em>debliecki</em>, <em>E</em>. <em>perminuta</em>, <em>E</em>. <em>porci</em>, <em>E</em>. <em>spinosa</em>, <em>E</em>. <em>suis</em>, and <em>Isospora suis</em>, commonly found in pigs of the North Eastern Hill (NEH) region of India. Utilizing a two-stage approach, segmentation of coccidia oocysts in microscopic images using convolutional neural networks (CNNs), followed by species identification by same network was carried out in this work. Resource-efficient models, including EfficientNetB0, EfficientNetB1, MobileNet, and MobileNetV2, within an encoder-decoder architecture were utilized to extract features. Transfer learning was applied to enhance model accuracy during training. Additionally, a marker-controlled watershed algorithm was implemented to separate touching cells, thus reducing misclassification. The results demonstrate that all the developed models effectively segmented/classified Coccidia species, achieving mean Intersection-over-Union (m-IoU) values exceeding 0.92, with individual class IoU scores above 0.90. MobileNetV2 exhibited the highest m-IoU of 0.95, followed by EfficientNetB1 with an m-IoU of 0.94. For classification, MobileNetV2 demonstrated the highest performance, with accuracy, precision, and recall values of 0.93, 0.96, and 0.96, respectively. EfficientNetB1 yielded an accuracy of 0.91. The developed mobile application, tested on new data, achieved an identification accuracy of 91.0 %. These findings highlight the potential of deep learning-based mobile applications in effectively identifying Coccidia species in pigs, thus, providing a promising solution to mitigate reliance on expert personnel and laborious time-consuming experiments in this domain.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110376"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the transmission of Anaplasma marginale by Amblyomma tonelliae","authors":"Matilde Nahime Mazzucco Panizza ,&nbsp;Evelina Luisa Tarragona ,&nbsp;Patrick Stephan Sebastian ,&nbsp;Fernando Sebastian Flores ,&nbsp;Atilio José Mangold ,&nbsp;Santiago Nava","doi":"10.1016/j.vetpar.2024.110380","DOIUrl":"10.1016/j.vetpar.2024.110380","url":null,"abstract":"<div><div>This study aimed to evaluate <em>A. marginale</em> transstadial (TST) and transovarial transmission (TOT) by <em>Amblyomma tonelliae</em> through vector competence assays and analysis of natural infection in free-living ticks. This three-host tick species was chosen as model because it is a usual parasite of cattle in all their parasitic stages, making them potential vectors through TST or TOT. Experiment 1 was performed to evaluate TST of <em>A. marginale</em> field strains and M1 strain, and the experiment 2 to evaluate TST and TOT of <em>A. marginale</em> S1P strain from an experimentally acutely infected calf. Free-living larvae, nymphs and adults of <em>A. tonelliae</em> were analyzed to evaluate natural infection with <em>A. marginale</em>. One nymph pool of the experiment 2 resulted positive (minimum infection rate of 1.25 %). However, the negative results of both vector competence assays and the molecular analysis of free-living ticks indicate that the transmission of <em>A. marginale</em> by <em>A. tonelliae</em> would not occur. This finding aligns with three of the four existing studies on the TST and TOT of <em>A. marginale</em> by ticks of the <em>Amblyomma</em> genus.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110380"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-13 partly induced by the NLRP3 inflammasome promotes Trichinella spiralis encapsulation in infected mice","authors":"Xuanrui Liu ,&nbsp;Bo Zhang ,&nbsp;Zhiyuan Zhang ,&nbsp;Xueting Wang ,&nbsp;Tongxuan Zhang ,&nbsp;Haibin Huang ,&nbsp;Chunwei Shi ,&nbsp;Wentao Yang ,&nbsp;Yanlong Jiang ,&nbsp;Xin Cao ,&nbsp;Jianzhong Wang ,&nbsp;Yan Zeng ,&nbsp;Chunfeng Wang ,&nbsp;Nan Wang ,&nbsp;Guilian Yang","doi":"10.1016/j.vetpar.2024.110386","DOIUrl":"10.1016/j.vetpar.2024.110386","url":null,"abstract":"<div><div><em>Trichinella spiralis</em> infection is a serious parasitic zoonosis in which a collagenous capsule surrounding the larva is developed in the striated muscle cells. However, the mechanism of <em>T. spiralis</em> encapsulation is currently poorly understood. It has been reported that <em>T. spiralis</em> infection can induce the production of IL-13 via the NLRP3 inflammasome, and it has also been suggested IL-13 thus produced may be involved in <em>T. spiralis</em> encapsulation. This research aimed to clarify the involvement of NLRP3 and IL-13 in the <em>T. spiralis</em> capsule formation process. IL-13 and NLRP3 inhibitors were used in a <em>T. spiralis</em> infected mouse model and in C₂C₁₂ cells to analyze the role of IL-13 and NLRP3 in encapsulation. The results showed that <em>T. spiralis</em> infection significantly increased the expression levels of IL-13 and collagen IV and VI. The production of collagen around the <em>T. spiralis</em> encapsulation zone was significantly inhibited when an IL-13 inhibitor was applied. Moreover, the expression levels of IL-13 and collagen IV and VI were significantly decreased by the NLRP3 inhibitor in vitro and in vivo. The above results indicated that NLRP3 can participate in the development of <em>T. spiralis</em> encapsulation by regulating IL-13 expression and stimulating collagen IV and VI synthesis during <em>T. spiralis</em> infection.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110386"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}