Veterinary parasitology最新文献

{"title":"Evaluation of Fas2-ELISA in milk and serum for the detection of Fasciola hepatica infection in cattle","authors":"MP Garcia-Lazaro ,&nbsp;E. Chavarry ,&nbsp;D. Zarate-Rendón ,&nbsp;P. Herrera-Velit ,&nbsp;JR Espinoza","doi":"10.1016/j.vetpar.2025.110615","DOIUrl":"10.1016/j.vetpar.2025.110615","url":null,"abstract":"<div><div><em>Fasciola hepatica</em> causes a significant loss in milk and meat production in cattle farming worldwide. The treatment and control of <em>F. hepatica</em> infection in cattle raised in regions with a high prevalence of the infection requires sensitive, specific, and affordable diagnostic tests. This study aims to evaluate two tests, Fas2-ELISA and ES-ELISA, for the diagnosis of fascioliasis in beef and dairy cattle from two livestock basins in Peru. Serum and fecal samples were collected from beef cattle slaughtered at an abattoir in Lima; serum, milk, and fecal samples were collected from <em>F. hepatica</em> naturally infected dairy cows in the San Lorenzo district, located in the Mantaro Andean Valley, and dairy cows raised in university stables in La Molina district in Lima. ES-ELISA detects IgG antibodies against <em>F. hepatica</em> excretory-secretory products (ES) from adult flukes, and Fas2-ELISA detects the Fas2 antigen, a cysteine proteinase purified from <em>F. hepatica</em> adult flukes. The diagnostic parameters for beef cattle were determined using inspection for <em>F. hepatica</em> flukes in the liver as a reference test. ES-ELISA for the detection of <em>F. hepatica</em> infection in beef cattle showed a sensitivity (Se) of 93.2 % (95 % CI, 83.5–98.1 %) and specificity (Sp) of 88 % (95 % CI, 80.4–93.5 %). Fas2-ELISA showed a Se and Sp of 98.3 % (95 % CI, 91–99 %) and 93.6 % (95 % CI, 87–97 %), respectively. The diagnostic parameters for detecting <em>F. hepatica</em> infection in milk samples by ES-ELISA showed a Se of 100 % (95 % CI, 92–100 %) and Sp of 83 % (95 % CI, 65–94 %), Fas2-ELISA showed a Se of 98 % (95 % CI, 89–100 %) and Sp of 93 % (95 % CI, 78–99 %) using coproscopy as a reference test. These results suggest that Fas2-ELISA could be a useful test for detecting <em>F. hepatica</em> infection in milk samples from dairy cattle and may be further developed as a bulk-tank milk (BTM) test to estimate the prevalence of <em>F. hepatica</em> infection in dairy herds.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110615"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental hyper-progesteronemia increases the egg shedding and fertility of Haemonchus contortus in sheep","authors":"Marco Antonio Muñoz-Guzmán,&nbsp;Adolfo Sánchez-Paredes,&nbsp;César Cuenca-Verde,&nbsp;María Guadalupe Prado-Ochoa,&nbsp;Omar Escobar-Chavarría,&nbsp;Fernando Alba-Hurtado","doi":"10.1016/j.vetpar.2025.110593","DOIUrl":"10.1016/j.vetpar.2025.110593","url":null,"abstract":"<div><div>Hyperprogesteronemia effects on the establishment and fertility of <em>Haemonchus contortus</em> were measured in an experimental infection in gonadectomized male lambs. Sixteen lambs were divided into two groups. Lambs from the progesterone (PG) group (n = 8) were implanted subcutaneously at week −2 with two devices (CIDR 330, Zoetis lab®) containing 600 mg of ovine progesterone. The second group (n = 8) was not implanted and served as the control. Lambs from both groups were inoculated intra-ruminally with 5000 L₃ of <em>H. contortus</em> at week 0. All lambs were humanely euthanized at week seven p.i.</div><div>All lambs in the PG group exhibited hyperprogesteronemia at the time of <em>H. contortus</em> infection. They had higher fecal egg and blood eosinophil counts than lambs in the control group (p &lt; 0.04). The female worms in the PG group had higher fertility (p &lt; 0.04) than those in the control group. No differences (p &gt; 0.05) were observed in adult worm numbers in the abomasum, female/male ratio, or antibody levels in both groups. The increase in serum progesterone levels in orchiectomized lambs at the time of infection stimulated an increase in <em>H. contortus</em> fertility, and consequently, in fecal egg counts. The above results show that the <em>H. contortus</em> parasite can recognize and respond to the presence of progesterone in vivo, strongly suggesting that this hormone plays a relevant role in the transregulation observed during peripartum rise.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110593"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chi-miR-146a promotes the propagation of Neospora caninum by targeting the TGF-β1/SMAD2 pathway","authors":"Jin-Ming Chen,&nbsp;Jing-Yu Li,&nbsp;Jia-Yi Jin,&nbsp;Xiao-Qi Bao,&nbsp;De-Liang Tao,&nbsp;Xiao-Yun Zhao,&nbsp;Jun-Ke Song,&nbsp;Xin Yang,&nbsp;Ying-Ying Fan,&nbsp;Guang-Hui Zhao","doi":"10.1016/j.vetpar.2025.110632","DOIUrl":"10.1016/j.vetpar.2025.110632","url":null,"abstract":"<div><div><em>Neospora caninum</em> is known to induce abortions in various domestic animals (especially cattle and sheep), leading to substantial economic losses in the livestock industry. However, effective strategies for controlling <em>N. caninum</em> infection remains elusive. The precise mechanisms underlying the interaction between <em>N. caninum</em> and its host are not fully elucidated. Our previous study showed an upregulated microRNA (miRNA), Chi-miR-146a, promoted the propagation of <em>N. caninum</em> tachyzoites in caprine endometrial epithelial cells (EECs). The present study investigated its regulatory function during <em>in vitro</em> infection of <em>N. caninum</em>. The expression of Chi-miR-146a was upregulated from 12 h post infection (hpi) to 48 hpi. One of target genes for Chi-miR-146a, <em>tgfb1</em>, was identified and the mRNA and protein levels were downregulated at 24 hpi and 48 hpi. Overexpression of <em>tgfb1</em> suppressed the propagation of <em>N. caninum</em> tachyzoites in caprine EECs. Further analysis indicated that Chi-miR-146a enhanced <em>in vitro</em> propagation of <em>N. caninum</em> propagation through suppressing the activation of the TGF-β1/SMAD2 signaling pathway. The findings in this study offer a novel theoretical framework for advancing our understanding on mechanisms of intracellular survival of <em>N. caninum</em> and devising preventive strategies against this pathogen.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110632"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological and molecular tools for environmental surveillance of canine echinococcosis: Steps toward a sustainable diagnostic algorithm","authors":"Héctor Gabriel Avila ,&nbsp;Lorena Evelina Lazzarini ,&nbsp;Luciano Ritossa ,&nbsp;Vilma Disalvo ,&nbsp;Verónica Roxana Flores ,&nbsp;Erio curto ,&nbsp;Fabián Zanini ,&nbsp;Gustavo Pedro Viozzi ,&nbsp;María Victoria Periago ,&nbsp;Nora Beatriz PIerangeli","doi":"10.1016/j.vetpar.2025.110623","DOIUrl":"10.1016/j.vetpar.2025.110623","url":null,"abstract":"<div><div>Canine echinococcosis (CaEc) surveillance has evolved from necropsy and arecoline purgation to the detection of coproantigens (cELISA) and genomic copro-DNA (cPCR and cLAMP). Each technique has advantages and disadvantages regarding biosafety, ethics, and costs. In Argentina, there is no consensus on CaEc surveillance tools or their suitability for low- and medium-complexity laboratories. The aim of this work was to compare the performance of techniques with different targets for CaEc surveillance, including cELISA, nested cPCR, and two cLAMPEGSL (2.0 and 3.0). Environmental canine fecal samples (n = 127) from endemic areas were analyzed using the four methods. Overall, Positive and Negative Percent Agreement (OPA, PPA, NPA) were evaluated. Sensitivity and specificity of each technique, and general prevalence were estimated using a Bayesian latent class model (BLCA). Both cLAMPEGSL3.0 and cPCR techniques showed higher OPA and NPA values than the cELISA, a validated method with very high NPV. Sensitivity estimates for each technique were: cELISA: 78.8 % (95 % CI: 56–94 %); cPCR 87.9 % (66–98 %); cLAMPEGSL2.0 65.6 % (29–96 %) and cLAMPEGSL3.0 86.3 % (50–99 %). While specificities estimates were: cELISA 55.7 % (46–66 %); cPCR 64.7 % (55–74 %); cLAMPEGSL2.0 57.9 % (47–68 %) and cLAMPEGSL3.0 62.4 % (52–73 %). The estimated general prevalence of CaEc was 13.1 % (9–18 %). This is the first study conducted in Argentina to compare the performance of four techniques with different targets for CaEc surveillance. Sensitivity and specificity of each technique, and general prevalence were estimated using a Bayesian Latent Class Analysis (BLCA) model. Using a BLCA model, both cPCR and cLAMPEGSL3.0 showed the best estimated sensitivity and specificity values. These results provide control programs with molecular tools suitable for use in medium- and low-complexity laboratories.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110623"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of β-tubulin isotypes in Uncinaria stenocephala and implications for benzimidazole resistance in hookworms","authors":"Thomas Stocker ,&nbsp;Jan Šlapeta","doi":"10.1016/j.vetpar.2025.110569","DOIUrl":"10.1016/j.vetpar.2025.110569","url":null,"abstract":"<div><div><em>Uncinaria stenocephala</em> is a widespread hookworm of dogs across Europe, Canada, southern Australia, and other temperate regions, where it often outnumbers infections caused by <em>Ancylostoma caninum</em>. Although a putative β-tubulin isotype-1 mutation associated with resistance has been detected in <em>U. stenocephala</em>, clinical resistance to benzimidazoles has not yet been confirmed. Benzimidazole resistance is primarily linked to single nucleotide polymorphisms (SNPs) in the β-tubulin isotype-1 gene; however, the β-tubulin genes of <em>U. stenocephala</em> have not been fully characterised. We aimed to identify β-tubulin genes and confirm the coding sequences for key residues (Q134, F167, E198, and F200) in the β-tubulin isotype-1 gene of the <em>U. stenocephala</em> genome. Two <em>U. stenocephala</em> specimens were subjected to Illumina sequencing, and species identity was confirmed through morphological and molecular analysis using ITS rDNA and <em>cox</em>1 markers. Genome assembly revealed the presence of β-tubulin isotype-1 (10 exons) and isotype-2 (9 exons), both homologous to β-tubulins from other hookworms (<em>A. caninum</em>, <em>A. ceylanicum</em>, <em>A. duodenale</em> and <em>Necator americanus</em>). The β-tubulin isotype-1 protein sequence of <em>U. stenocephala</em> contained two variable residues (S37Q and G441A) compared to other hookworm sequences. While the isotype-2 protein sequence was conserved among <em>Ancylostoma</em> species, <em>U. stenocephala</em> exhibited six distinct polymorphisms (E39D, T40S, N115S, L130I, A287S, T439G). The benzimidazole-susceptible residues (Q134, F167, E198, F200) were present in the β-tubulin isotype-1 protein sequence. Characterisation of the complete coding regions of β-tubulin isotypes 1 and 2 enables population-level screening for benzimidazole resistance-associated SNPs and provides a foundation for future epidemiological studies in <em>U. stenocephala</em>.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110569"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural characterization of bioaccumulation and migration of Ag nanoparticles in host-parasite organisms","authors":"Fuad Rzayev ,&nbsp;Eldar Gasimov ,&nbsp;Ali Nasirov ,&nbsp;Sarvinaz Hajiyeva ,&nbsp;Mehri Seyidbeyli ,&nbsp;Aladdin Eyvazov ,&nbsp;Gunay Rzayeva","doi":"10.1016/j.vetpar.2025.110554","DOIUrl":"10.1016/j.vetpar.2025.110554","url":null,"abstract":"<div><div>In an era of developing nanotechnologies, studying the bioaccumulation and migration of nanoparticles in various components of the ecosystem, and the varying degrees of pathology they cause in living organisms - is important. In the present study, the bioaccumulation and migration of nanoparticles in both the host and parasite were examined by light and electron microscopy, focusing on the nematode <em>Heterakis dispar</em>, which causes serious damage to the organism of the domestic goose. Silver nanoparticles (Ag NPs) were administered to birds infected with <em>H. dispar</em> at a concentration of 100 μg/ml (total volume 10 ml). The parasites, as well as intestine, liver, and skeletal striated muscle of the host, were examined by histological methods and electron microscopy. It was found that the sizes of AgNPs at the free state were ranging from 9.03 to 23.82 nm (13.88 ± 0.48 nm), while in the parasite organism they were up to 14 nm, and in birds they did not exceed 13 nm. Nanoparticles bioaccumulated in the parasite, causing pathological changes. AgNPs were observed to migrate through the integumentary tissue of the parasite into the pseudocoelomic cavity organs. Various pathological changes occurred in the structural elements of the intestine, liver, and skeletal striated muscle of birds due to the action of AgNPs. Nanoparticles entered the cytoplasm of erythrocytes located in the lumen of the vessels in the submucosal layer of the goose intestine and subsequently migrated to the liver and striated muscle.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110554"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal patterns of faecal egg counts and gastrointestinal nematode species composition in Scottish dairy calves","authors":"Paul Campbell,&nbsp;Jennifer McIntyre,&nbsp;Kerry O’Neill,&nbsp;Andrew Forbes,&nbsp;Roz Laing,&nbsp;Kathryn Ellis","doi":"10.1016/j.vetpar.2025.110574","DOIUrl":"10.1016/j.vetpar.2025.110574","url":null,"abstract":"<div><div>Gastrointestinal nematode (GIN) infections impact livestock production globally. In pasture-based systems, GIN infections are ubiquitous, typically comprising co-infections with several different species within a single host. Nematode species vary in their epidemiology, pathogenicity, and anthelmintic sensitivity, which in turn can be influenced by weather, host factors, and management practices. The epidemiology of parasitic gastroenteritis in young cattle in temperate regions has been thoroughly researched. However, many studies were conducted more than fifty years ago, before the advent of modern molecular techniques and the widespread use of macrocyclic lactone (ML) anthelmintics. This study's objective was to survey dairy farms with different management profiles, using faecal egg counts (FEC) and GIN L₃ identification, to determine if any changes had occurred since these original studies. The longitudinal study of 23 Scottish dairy farms included 131 monthly sampling points, from which 1967 individual FECs were conducted, and a minimum of 94 L₃ from pooled coprocultures identified by PCR (n = 13,297) per visit. Species composition and FEC followed expected patterns, yet varied considerably in relation to management and anthelmintic use; <em>Cooperia oncophora</em> was more abundant earlier in the grazing season, while <em>Ostertagia ostertagi</em> became more abundant as the season progressed. Other GIN observed included Trichostrongylus spp., Oesophagostomum spp., and <em>Haemonchus contortus</em>. The majority of farms relied entirely on ML products and had done so for many years. Farmer concerns regarding anthelmintic resistance were minimal, and few farms routinely employed FECs to aid management decisions. Regardless of treatment strategy, the groups exhibited no evidence of clinical disease, and FECs remained relatively low throughout, even on farms not using any anthelmintic treatment (0–480 eggs per gram).</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110574"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144810330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial genetic stability of Echinococcus granulosus s.s. across life stages and hosts in an experimental infection model.","authors":"Figen Celik, Muhammet Uslug, Sami Simsek","doi":"10.1016/j.vetpar.2025.110576","DOIUrl":"10.1016/j.vetpar.2025.110576","url":null,"abstract":"<p><p>Cystic echinococcosis, caused by Echinococcus granulosus sensu stricto (G1/G3), is a major zoonosis with a complex transmission cycle. This study aimed to evaluate the mitochondrial genetic stability of E. granulosus s.s. across different life stages and host species using a controlled experimental infection model. To achieve this, mitochondrial genetic variation was analyzed separately in protoscoleces (naturally infected sheep), adult worms (experimentally infected dogs), and hydatid cysts (experimentally infected lambs), to assess within- and between-group genetic stability. Mitochondrial gene regions CO1 (875 bp) and NADH1 (1009 bp) were amplified and sequenced. Phylogenetic, haplotype, and neutrality analyses revealed that all isolates clustered within a single monophyletic group. While CO1 showed moderate haplotype (Hd = 0.730) and low nucleotide diversity (π = 0.00267), NADH1 displayed higher haplotypic and nucleotide diversity (Hd = 0.983; π = 0.00876). Significantly negative Fu's Fs values for both markers suggested a recent demographic expansion, potentially driven by clonal amplification under low evolutionary pressure. Despite the presence of several haplotypes, no host- or tissue-specific genetic differentiation was observed. These findings demonstrate the genetic continuity of E. granulosus s.s. throughout its life cycle and confirm the suitability of mitochondrial markers for molecular tracking and epidemiological studies in endemic regions.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"110576"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of Glutathione-S-transferase levels as marker of hepatic damage in chronic fasciolosis in cattle.","authors":"Al-Hassan Mohammed Mostafa, Gehan Mohammed Sayed, Ali Ali Hassan Al-Ezzi, Alaa Eldin Kamal","doi":"10.1016/j.vetpar.2025.110577","DOIUrl":"10.1016/j.vetpar.2025.110577","url":null,"abstract":"<p><p>There are various parasite pathogens that infect cattle, buffaloes, sheep, and goats, with fasciolosis being one of the most common. This article established a glutathione s-transferase (GST) evaluation for Fasciola spp infection and explored its application value as diagnostic tool for assess the hepatic damage, linking it with histopathological findings and the lesion score for the degree of infection with Fasciola spp. Thirty-two animals of cattle species were assigned. The investigation gathered fecal samples for sedimentation counts, blood samples for serum (GST quantification), and two tissue samples from a fasciolosis-infected liver (one in formalin for histopathological examination and the other for homogenate preparation for GST estimation). The animals were divided into four groups (8 each): Severely infected group (SG), Moderate infected group (MoG), Mild infected group (MiG), and non-infected group (C-ve). In sedimentation, SG showed a significantly higher fecal egg count but lower serum and homogenate GST values compared to other groups, while serum and homogenate GST values were lower in SG and MoG than in other groups. MiG group had higher values than C-ve, MoG and SG groups, respectively. Furthermore, pathological lesion scores were gradually increased from low to high in groups viz. (MiG, MoG, and SG, respectively). Hepatic fasciolosis is still a big economic problem in the veterinary field. GST could assess hepatic damage in the case of chronic fasciolosis.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"110577"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural acaricides against Hyalomma tick species: A review of efficacy, mechanisms and applications in integrated pest management","authors":"Mourad Ben Said ,&nbsp;Myriam Kratou ,&nbsp;Hanène Belkahia","doi":"10.1016/j.vetpar.2025.110580","DOIUrl":"10.1016/j.vetpar.2025.110580","url":null,"abstract":"<div><div>The increasing occurrence of <em>Hyalomma</em> ticks, especially <em>H. scupense</em>, <em>H. anatolicum</em>, <em>H. marginatum</em> and <em>H. dromedarii</em>, poses a serious threat to livestock health and production, prompting research on innovative tick control strategies. Hence, this review explores the efficacy of natural acaricides as a green alternative to synthetic acaricides. Several recent studies have demonstrated promising acaricidal activity, particularly of plant-derived essential oils and extracts, against these <em>Hyalomma</em> species. In this reviex, we examine the active ingredients, testing methods, and overall efficacy of such natural products in controlling tick infestations. The review underscores the need to close existing research gaps, including the importance of <em>in vivo</em> studies, long-term efficacy evaluations, and exploration of the mechanisms of action of these natural acaricides. In addition, the use of natural compounds that are not harmful to non-target organisms and the environment is discussed, as they have ecologically beneficial effects. Despite these advances, standardized protocols for evaluating natural acaricides and assessing their integration into existing tick management programs remain underdeveloped. This review highlights the potential of natural acaricides in livestock health and its correlation with ecological issues, emphasizing the need for sustainable tick management through these innovative strategies that warrant further investigation and development to control ticks of this genus.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"Article 110580"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}