Phytochemical and acaricidal analysis of lavender (Lavandula angustifolia) and sage (Salvia officinalis) essentials oils against Rhipicephalus microplus ticks: An in vitro and in vivo study

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-06-04 DOI:10.1016/j.vetpar.2025.110523

Shivam Singh Mehrotra , Mukesh Shakya , Mamta Singh , R.K. Bagherwal , Anil Kumar Sharma , Hemant Kumar Mehta

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Abstract

Based on earlier reports on acaricidal and repellent properties of Lavandula angustifolia and Salvia officinalis plants against various ectoparasites, including tick species, the present study was carried out using these essential oils (EOs) to explore the possibility of developing a potent herbal anti-tick formulation to manage cattle tick Rhipicephalus microplus. The EOs were evaluated against different life stages of ticks to provide a complete solution for tick control. The oils were also subjected to GC-MS analysis for their phytochemical constituents, which revealed a total of 125 compounds in lavender oil, predominantly consisting of alpha-pinene (10.24 %), D-limonene (7.50 %), 1,3,6-octatriene, 3,7-dimethyl-(Z) (6.48 %) and linalool (6.43 %), while sage oil contained 92 compounds, with linalool and linalyl acetate with an area percentage of 11.03 % and 9.83 % being the most abundant. All the treated engorged ticks were killed at 5 and 10 % EOs of L. angustifolia and S. officinalis with a significant reduction in oviposition in different concentrations. Both EOs were also effective against 83–100 % larvae at a 5 % dose in larval immersion test. Additionally, the study also examined the hatching inhibition of R. microplus eggs treated with both essential oils and achieved 100 % hatching inhibition at 5 % concentration. The repellency index (RI) indicated effective repellent activity with L. angustifolia having 0.0 ± 0.0 and S. officinalis 0.6 ± 0.15 at RI 10 %. Hematological and biochemical parameters showed no significant changes and no skin irritation in topically treated animals, suggesting the oils' safety for livestock use. The in vivo efficacy of both EOs was over 80 % after 72 h under patch treatment of naturally infested cattle. This research highlights the potential of lavender and sage essential oils as viable alternatives to develop an eco-friendly solution for tick management.

查看原文本刊更多论文

薰衣草（Lavandula angustifolia）和鼠尾草（Salvia officinalis）精油对微头蜱的植物化学和杀螨性分析：体外和体内研究

基于先前关于薰衣草和鼠尾草植物对各种体外寄生虫（包括蜱虫）的杀螨和驱虫特性的报道，本研究使用这些精油（EOs）进行，以探索开发一种有效的草药抗蜱制剂来管理牛蜱微头虫的可能性。对不同生命阶段的蜱虫进行评估，为蜱虫控制提供完整的解决方案。经GC-MS分析，薰衣草精油中共含有125种化合物，主要为α -蒎烯（10.24 %）、d -柠檬烯（7.50 %）、1,3,6-辛三烯、3,7-二甲基-(Z)（6.48 %）和芳樟醇（6.43 %），鼠尾草精油中含有92种化合物，其中芳樟醇和乙酸芳樟醇含量最高，分别为11.03 %和9.83 %。灌血蜱在灌血浓度为5%和10%（ %）时全部被杀，不同浓度灌血蜱的产卵量均显著降低。在幼虫浸泡试验中，以5 %的剂量对83 ~ 100 %的幼虫均有效。此外，本研究还检测了两种精油处理后的微卵卵的孵化抑制作用，在5 %的浓度下，孵化抑制率达到100 %。驱避指数（RI）为10 %时，钉螺的驱避活性分别为0.0 ± 0.0和0.6 ± 0.15。局部处理动物的血液学和生化参数无明显变化，皮肤无刺激，表明该油对牲畜使用是安全的。对自然感染的牛进行贴片处理72 h后，两种EOs的体内效率均在80% %以上。这项研究强调了薰衣草和鼠尾草精油作为可行替代品的潜力，可以开发出一种环保的蜱虫管理解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.